SDO1 cDNA ORF clone, Saccharomyces cerevisiae S288C

Gene
Clones
gRNAs

Summary

Gene Symbol	SDO1
Entrez Gene ID	850709
Full Name	guanine nucleotide exchange factor SDO1
Gene Type	protein-coding
Organism	Saccharomyces cerevisiae S288C

mRNA and Protein(s)

mRNA	Protein	Name
NM_001181909.1	NP_013122.1	guanine nucleotide exchange factor SDO1

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	SBDS	XP_848632.1
Drosophila melanogaster (fruit fly)	CG8549	NP_001261482.1
Kluyveromyces lactis	KLLA0F15532g	XP_455778.1
Neurospora crassa	NCU00476	XP_956795.1
Pan troglodytes (chimpanzee)	SBDS	XP_519568.2
Gallus gallus (chicken)	SBDS	NP_001006211.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP007882	XP_317609.3
Caenorhabditis elegans (roundworm)	sbds-1	NP_497226.2
Gallus gallus (chicken)	LOC100858362	XP_003642436.1
Eremothecium gossypii	AGOS_AAL154C	NP_982388.1
Schizosaccharomyces pombe (fission yeast)	sdo1	NP_593869.1
Magnaporthe oryzae (rice blast fungus)	MGG_05662	XP_003710560.1
Homo sapiens (human)	SBDS	NP_057122.2
Rattus norvegicus (Norway rat)	Sbds	NP_001008290.1
Danio rerio (zebrafish)	sbds	NP_957415.1
Arabidopsis thaliana (thale cress)	AT1G43860	NP_564488.1
Oryza sativa (rice)	Os08g0107500	NP_001060798.1
Bos taurus (cattle)	SBDS	NP_001029611.1
Mus musculus (house mouse)	Sbds	NP_075737.1
Saccharomyces cerevisiae (baker's yeast)	SDO1	NP_013122.1
Xenopus tropicalis (tropical clawed frog)	sbds	NP_001004953.1

Gene Ontology
Bibliography

Related articles in PubMed

The nucleotide sequence of Saccharomyces cerevisiae chromosome XII.
Johnston M, Hillier L, Riles L, Albermann K, Andr? B, Ansorge W, Benes V, Br?ckner M, Delius H, Dubois E, D?sterh?ft A, Entian KD, Floeth M, Goffeau A, Hebling U, Heumann K, Heuss-Neitzel D, Hilbert H, Hilger F, Kleine K, K?tter P, Louis EJ, Messenguy F, Mewes HW, Hoheisel JD
Nature387(6632 Suppl)87-90(1997 May)

Life with 6000 genes.
Goffeau A, Barrell BG, Bussey H, Davis RW, Dujon B, Feldmann H, Galibert F, Hoheisel JD, Jacq C, Johnston M, Louis EJ, Mewes HW, Murakami Y, Philippsen P, Tettelin H, Oliver SG
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

data support a conformational signal-relay cascade during late-stage 60S maturation, involving uL16, Sdo1p, and Efl1p, which interrogates the functional P-site to control the departure of the anti-association factor eIF6.
Title: Structural dynamics of the yeast Shwachman-Diamond syndrome protein (Sdo1) on the ribosome and its implication in the 60S subunit maturation.

Cells lacking Sdo1 exhibit reduced tolerance to various stress conditions.
Title: Deletion of Mitochondrial Porin Alleviates Stress Sensitivity in the Yeast Model of Shwachman-Diamond Syndrome.

Sdo1 protein acts as a nucleotide exchange factor that stabilizes binding to GTP for yeast GTPase.
Title: Guanine nucleotide exchange in the ribosomal GTPase EFL1 is modulated by the protein mutated in the Shwachman-Diamond syndrome.

Sdo1p binds RNA directly, suggesting that it may associate with the ribosomal subunits also through RNA interaction.
Title: Sdo1p, the yeast orthologue of Shwachman-Bodian-Diamond syndrome protein, binds RNA and interacts with nuclear rRNA-processing factors.

Sdo1 appears to function within a pathway containing elongation factor-like 1, and together they control translational activation of ribosomes.
Title: The Shwachman-Bodian-Diamond syndrome protein mediates translational activation of ribosomes in yeast.

The following SDO1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the SDO1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OSi00374	NM_001181909.1 Latest version!	Saccharomyces cerevisiae S288C guanine nucleotide exchange factor SDO1 (SDO1), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$174.30 $249.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

SDO1 ( NM_001181909.1 ) cDNA ORF clone, Saccharomyces cerevisiae S288C -> NP_013122.1 Saccharomyces cerevisiae S288C guanine nucleotide exchange factor SDO1 (SDO1), partial mRNA.

Price & Availability↑

CloneID	OSi00374
Clone ID Related Accession (Same CDS sequence)	NM_001181909.1
Accession Version	NM_001181909.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 753bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-31
Organism	Saccharomyces cerevisiae S288C
Product	guanine nucleotide exchange factor SDO1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by SGD. This record is derived from an annotated genomic sequence (NC_001144). ##Genome-Annotation-Data-START## Annotation Provider :: SGD Annotation Status :: Full Annotation Annotation Version :: R64-2-1 URL :: http://www.yeastgenome.org/ ##Genome-Annotation-Data-END## COMPLETENESS: incomplete on both ends.

ATGCCTATCA ATCAACCGTC GGGACAGATT AAGTTAACTA ATGTTTCATT AGTGAGATTA 
AAAAAGGCTC GCAAGAGATT TGAAGTCGCG TGCTACCAAA ATAAGGTCCA AGACTATCGA 
AAAGGAATTG AAAAAGATCT GGATGAAGTG CTGCAGATAC ATCAAGTTTT CATGAATGTT 
TCGAAAGGCT TGGTTGCTAA TAAGGAAGAT CTACAAAAGT GTTTCGGGAC TACAAATGTT 
GATGATGTCA TCGAGGAGAT CATGCATAAG GGAGAGATTC AATTATCGGA AAAAGAAAGA 
CAATTAATGC TTAATAAAGT TAATAACGAA ATGCTTACAA TAGTTAGCGC TAAATGTATA 
AATCCTGTGT CAAAGAAAAG ATATCCGCCC ACAATGATCC ACAAAGCTTT ACAGGAACTG 
AAGTTCAGCC CTGTTATTAA CAAACCTGCC AAATTGCAAG CTTTAGAGGC TATCAAATTA 
TTGGTTTCTA AGCAAATTAT TCCAATAGTA AGGGCGAAGA TGAAAGTCAA AGTGGCCATA 
AGTGAGCCAT CTAGACAACC CGAACTTATT GAGAAGATTA GTAAATTGAT AGCTTCATCG 
CCTGGAGAGT CAACGAAGCC GGAATTGGAT CCTTGGACAT GTACAGGTCT TATCGATCCT 
GTCAATTATA GAGATTTGAT GACCCTATGT GATAAAAAGG GAACTGTGCA AGTCTTGGAT 
ATGGCAGTCA TAGATAATAC AACGCATAAC TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_013122.1
CDS	1..753
Translation	MPINQPSGQIKLTNVSLVRLKKARKRFEVACYQNKVQDYRKGIEKDLDEVLQIHQVFMNVSKGLVANKEDLQKCFGTTNVDDVIEEIMHKGEIQLSEKERQLMLNKVNNEMLTIVSAKCINPVSKKRYPPTMIHKALQELKFSPVINKPAKLQALEAIKLLVSKQIIPIVRAKMKVKVAISEPSRQPELIEKISKLIASSPGESTKPELDPWTCTGLIDPVNYRDLMTLCDKKGTVQVLDMAVIDNTTHN

Target ORF information:

RefSeq Version	NM_001181909.1
Organism	Saccharomyces cerevisiae S288C
Definition	Saccharomyces cerevisiae S288C guanine nucleotide exchange factor SDO1 (SDO1), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001181909.1

ORF Insert Sequence:

ATGCCTATCA ATCAACCGTC GGGACAGATT AAGTTAACTA ATGTTTCATT AGTGAGATTA 
AAAAAGGCTC GCAAGAGATT TGAAGTCGCG TGCTACCAAA ATAAGGTCCA AGACTATCGA 
AAAGGAATTG AAAAAGATCT GGATGAAGTG CTGCAGATAC ATCAAGTTTT CATGAATGTT 
TCGAAAGGCT TGGTTGCTAA TAAGGAAGAT CTACAAAAGT GTTTCGGGAC TACAAATGTT 
GATGATGTCA TCGAGGAGAT CATGCATAAG GGAGAGATTC AATTATCGGA AAAAGAAAGA 
CAATTAATGC TTAATAAAGT TAATAACGAA ATGCTTACAA TAGTTAGCGC TAAATGTATA 
AATCCTGTGT CAAAGAAAAG ATATCCGCCC ACAATGATCC ACAAAGCTTT ACAGGAACTG 
AAGTTCAGCC CTGTTATTAA CAAACCTGCC AAATTGCAAG CTTTAGAGGC TATCAAATTA 
TTGGTTTCTA AGCAAATTAT TCCAATAGTA AGGGCGAAGA TGAAAGTCAA AGTGGCCATA 
AGTGAGCCAT CTAGACAACC CGAACTTATT GAGAAGATTA GTAAATTGAT AGCTTCATCG 
CCTGGAGAGT CAACGAAGCC GGAATTGGAT CCTTGGACAT GTACAGGTCT TATCGATCCT 
GTCAATTATA GAGATTTGAT GACCCTATGT GATAAAAAGG GAACTGTGCA AGTCTTGGAT 
ATGGCAGTCA TAGATAATAC AACGCATAAC TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The nucleotide sequence of Saccharomyces cerevisiae chromosome XII.
Nature387(6632 Suppl)87-90(1997 May)
Johnston M,Hillier L,Riles L,Albermann K,Andr? B,Ansorge W,Benes V,Br?ckner M,Delius H,Dubois E,D?sterh?ft A,Entian KD,Floeth M,Goffeau A,Hebling U,Heumann K,Heuss-Neitzel D,Hilbert H,Hilger F,Kleine K,K?tter P,Louis EJ,Messenguy F,Mewes HW,Hoheisel JD

Life with 6000 genes.
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)
Goffeau A,Barrell BG,Bussey H,Davis RW,Dujon B,Feldmann H,Galibert F,Hoheisel JD,Jacq C,Johnston M,Louis EJ,Mewes HW,Murakami Y,Philippsen P,Tettelin H,Oliver SG

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SDO1 cDNA ORF clone, Saccharomyces cerevisiae S288C

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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