ORF » Species Summary » Saccharomyces cerevisiae S288C » LEU2 cDNA ORF clone

LEU2 cDNA ORF clone, Saccharomyces cerevisiae S288C

The following LEU2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LEU2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OSi00065 NM_001178665.1
Latest version!		 Saccharomyces cerevisiae S288C 3-isopropylmalate dehydrogenase (LEU2), partial mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $258.30
$369.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    LEU2 ( NM_001178665.1 ) cDNA ORF clone, Saccharomyces cerevisiae S288C -> NP_009911.2 Saccharomyces cerevisiae S288C 3-isopropylmalate dehydrogenase (LEU2), partial mRNA.
    Price & Availability↑
    CloneID OSi00065
    Clone ID Related Accession (Same CDS sequence) NM_001178665.1
    Accession Version NM_001178665.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1095bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-10-31
    Organism Saccharomyces cerevisiae S288C
    Product 3-isopropylmalate dehydrogenase
    Comment Comment: REVIEWED REFSEQ: This record has been curated by SGD. This record is derived from an annotated genomic sequence (NC_001135). ##Genome-Annotation-Data-START## Annotation Provider :: SGD Annotation Status :: Full Annotation Annotation Version :: R64-2-1 URL :: http://www.yeastgenome.org/ ##Genome-Annotation-Data-END## COMPLETENESS: incomplete on both ends. 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    ATGTCTGCCC CTAAGAAGAT CGTCGTTTTG CCAGGTGACC ACGTTGGTCA AGAAATCACA 
    GCCGAAGCCA TTAAGGTTCT TAAAGCTATT TCTGATGTTC GTTCCAATGT CAAGTTCGAT 
    TTCGAAAATC ATTTAATTGG TGGTGCTGCT ATCGATGCTA CAGGTGTTCC ACTTCCAGAT 
    GAGGCGCTGG AAGCCTCCAA GAAGGCTGAT GCCGTTTTGT TAGGTGCTGT GGGTGGTCCT 
    AAATGGGGTA CCGGTAGTGT TAGACCTGAA CAAGGTTTAC TAAAAATCCG TAAAGAACTT 
    CAATTGTACG CCAACTTAAG ACCATGTAAC TTTGCATCCG ACTCTCTTTT AGACTTATCT 
    CCAATCAAGC CACAATTTGC TAAAGGTACT GACTTCGTTG TTGTCAGAGA ATTAGTGGGA 
    GGTATTTACT TTGGTAAGAG AAAGGAAGAC GATGGTGATG GTGTCGCTTG GGATAGTGAA 
    CAATACACCG TTCCAGAAGT GCAAAGAATC ACAAGAATGG CCGCTTTCAT GGCCCTACAA 
    CATGAGCCAC CATTGCCTAT TTGGTCCTTG GATAAAGCTA ATGTTTTGGC CTCTTCAAGA 
    TTATGGAGAA AAACTGTGGA GGAAACCATC AAGAACGAAT TCCCTACATT GAAGGTTCAA 
    CATCAATTGA TTGATTCTGC CGCCATGATC CTAGTTAAGA ACCCAACCCA CCTAAATGGT 
    ATTATAATCA CCAGCAACAT GTTTGGTGAT ATCATCTCCG ATGAAGCCTC CGTTATCCCA 
    GGTTCCTTGG GTTTGTTGCC ATCTGCGTCC TTGGCCTCTT TGCCAGACAA GAACACCGCA 
    TTTGGTTTGT ACGAACCATG CCACGGTTCT GCTCCAGATT TGCCAAAGAA TAAGGTCAAC 
    CCTATCGCCA CTATCTTGTC TGCTGCAATG ATGTTGAAAT TGTCATTGAA CTTGCCTGAA 
    GAAGGTAAGG CCATTGAAGA TGCAGTTAAA AAGGTTTTGG ATGCAGGTAT CAGAACTGGT 
    GATTTAGGTG GTTCCAACAG TACCACCGAA GTCGGTGATG CTGTCGCCGA AGAAGTTAAG 
    AAAATCCTTG CTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_009911.2
    CDS1..1095
    Translation

    Target ORF information:

    RefSeq Version NM_001178665.1
    Organism Saccharomyces cerevisiae S288C
    Definition Saccharomyces cerevisiae S288C 3-isopropylmalate dehydrogenase (LEU2), partial mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 NM_001178665.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    ATGTCTGCCC CTAAGAAGAT CGTCGTTTTG CCAGGTGACC ACGTTGGTCA AGAAATCACA 
    GCCGAAGCCA TTAAGGTTCT TAAAGCTATT TCTGATGTTC GTTCCAATGT CAAGTTCGAT 
    TTCGAAAATC ATTTAATTGG TGGTGCTGCT ATCGATGCTA CAGGTGTTCC ACTTCCAGAT 
    GAGGCGCTGG AAGCCTCCAA GAAGGCTGAT GCCGTTTTGT TAGGTGCTGT GGGTGGTCCT 
    AAATGGGGTA CCGGTAGTGT TAGACCTGAA CAAGGTTTAC TAAAAATCCG TAAAGAACTT 
    CAATTGTACG CCAACTTAAG ACCATGTAAC TTTGCATCCG ACTCTCTTTT AGACTTATCT 
    CCAATCAAGC CACAATTTGC TAAAGGTACT GACTTCGTTG TTGTCAGAGA ATTAGTGGGA 
    GGTATTTACT TTGGTAAGAG AAAGGAAGAC GATGGTGATG GTGTCGCTTG GGATAGTGAA 
    CAATACACCG TTCCAGAAGT GCAAAGAATC ACAAGAATGG CCGCTTTCAT GGCCCTACAA 
    CATGAGCCAC CATTGCCTAT TTGGTCCTTG GATAAAGCTA ATGTTTTGGC CTCTTCAAGA 
    TTATGGAGAA AAACTGTGGA GGAAACCATC AAGAACGAAT TCCCTACATT GAAGGTTCAA 
    CATCAATTGA TTGATTCTGC CGCCATGATC CTAGTTAAGA ACCCAACCCA CCTAAATGGT 
    ATTATAATCA CCAGCAACAT GTTTGGTGAT ATCATCTCCG ATGAAGCCTC CGTTATCCCA 
    GGTTCCTTGG GTTTGTTGCC ATCTGCGTCC TTGGCCTCTT TGCCAGACAA GAACACCGCA 
    TTTGGTTTGT ACGAACCATG CCACGGTTCT GCTCCAGATT TGCCAAAGAA TAAGGTCAAC 
    CCTATCGCCA CTATCTTGTC TGCTGCAATG ATGTTGAAAT TGTCATTGAA CTTGCCTGAA 
    GAAGGTAAGG CCATTGAAGA TGCAGTTAAA AAGGTTTTGG ATGCAGGTAT CAGAACTGGT 
    GATTTAGGTG GTTCCAACAG TACCACCGAA GTCGGTGATG CTGTCGCCGA AGAAGTTAAG 
    AAAATCCTTG CTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

  • PubMed

    Life with 6000 genes.
    Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)
    Goffeau A,Barrell BG,Bussey H,Davis RW,Dujon B,Feldmann H,Galibert F,Hoheisel JD,Jacq C,Johnston M,Louis EJ,Mewes HW,Murakami Y,Philippsen P,Tettelin H,Oliver SG


    The complete DNA sequence of yeast chromosome III.
    Nature357(6373)38-46(1992 May)
    Oliver SG,van der Aart QJ,Agostoni-Carbone ML,Aigle M,Alberghina L,Alexandraki D,Antoine G,Anwar R,Ballesta JP,Benit P


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