MOT2 cDNA ORF clone, Saccharomyces cerevisiae S288C

Gene
Clones
gRNAs

Summary

Gene Symbol	MOT2
Entrez Gene ID	856799
Full Name	CCR4-NOT core ubiquitin-protein ligase subunit MOT2
Synonyms	NOT4,SIG1
Gene Type	protein-coding
Organism	Saccharomyces cerevisiae S288C

mRNA and Protein(s)

mRNA	Protein	Name
NM_001178959.3	NP_010991.3	CCR4-NOT core ubiquitin-protein ligase subunit MOT2

Pathways from BioSystems

Homologies

Eremothecium gossypii	AGOS_ADL064W	NP_984032.1
Kluyveromyces lactis	KLLA0C08041g	XP_452558.1
Saccharomyces cerevisiae (baker's yeast)	MOT2	NP_010991.3

Gene Ontology
Bibliography

Related articles in PubMed

The nucleotide sequence of Saccharomyces cerevisiae chromosome V.
Dietrich FS, Mulligan J, Hennessy K, Yelton MA, Allen E, Araujo R, Aviles E, Berno A, Brennan T, Carpenter J, Chen E, Cherry JM, Chung E, Duncan M, Guzman E, Hartzell G, Hunicke-Smith S, Hyman RW, Kayser A, Komp C, Lashkari D, Lew H, Lin D, Mosedale D, Davis RW
Nature387(6632 Suppl)78-81(1997 May)

Life with 6000 genes.
Goffeau A, Barrell BG, Bussey H, Davis RW, Dujon B, Feldmann H, Galibert F, Hoheisel JD, Jacq C, Johnston M, Louis EJ, Mewes HW, Murakami Y, Philippsen P, Tettelin H, Oliver SG
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Not4 is required for global translational repression under nutritional limitations and especially for repression of mRNAs that cause transient ribosome stalling.
Title: Not4-dependent translational repression is important for cellular protein homeostasis in yeast.

Screening a variety of mutants involved in ubiquitin-mediated proteolysis demonstrated an important role for the nuclear ubiquitin ligase Not4 in Yap1 degradation. Not4 was found to bind to Yap1 in an oxidant-stimulated fashion
Title: Proteolytic degradation of the Yap1 transcription factor is regulated by subcellular localization and the E3 ubiquitin ligase Not4.

results identify a stress-induced proteolytic pathway regulating cyclin C that requires nuclear to cytoplasmic relocalization and Not4p-mediated ubiquitylation
Title: Oxidative-stress-induced nuclear to cytoplasmic relocalization is required for Not4-dependent cyclin C destruction.

Not4 is required for appropriate association of the Not5 subunit of the Ccr4-Not complex with polysomes and for ubiquitination of Rps7A.
Title: Presence of Not5 and ubiquitinated Rps7A in polysome fractions depends upon the Not4 E3 ligase.

results characterize Ecm29 as a proteasome chaperone whose appropriate interaction with the proteasome requires Not4
Title: Not4 E3 ligase contributes to proteasome assembly and functional integrity in part through Ecm29.

The following MOT2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the MOT2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OSi05563	NM_001178959.3 Latest version!	Saccharomyces cerevisiae S288C CCR4-NOT core ubiquitin-protein ligase subunit MOT2 (MOT2), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

MOT2 ( NM_001178959.3 ) cDNA ORF clone, Saccharomyces cerevisiae S288C -> NP_010991.3 Saccharomyces cerevisiae S288C CCR4-NOT core ubiquitin-protein ligase subunit MOT2 (MOT2), partial mRNA.

Price & Availability↑

CloneID	OSi05563
Clone ID Related Accession (Same CDS sequence)	NM_001178959.3
Accession Version	NM_001178959.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1764bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-31
Organism	Saccharomyces cerevisiae S288C
Product	CCR4-NOT core ubiquitin-protein ligase subunit MOT2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by SGD. This record is derived from an annotated genomic sequence (NC_001137). On Jul 30, 2012 this sequence version replaced NM_001178959.2. ##Genome-Annotation-Data-START## Annotation Provider :: SGD Annotation Status :: Full Annotation Annotation Version :: R64-2-1 URL :: http://www.yeastgenome.org/ ##Genome-Annotation-Data-END## COMPLETENESS: incomplete on both ends.

ATGATGAATC CACACGTTCA AGAAAATTTG CAAGCAATCC ACAACGCCTT AAGCAATTTT 
GATACGTCAT TTTTATCGGA GGATGAAGAA GATTATTGCC CTCTTTGTAT TGAACCAATG 
GATATTACTG ATAAAAATTT TTTTCCTTGT CCCTGTGGTT ATCAAATTTG TCAATTTTGC 
TACAATAATA TCAGACAAAA TCCAGAATTA AATGGCCGTT GCCCAGCATG TCGTCGTAAA 
TATGATGACG AGAACGTCAG ATACGTCACA TTATCTCCGG AGGAGTTAAA AATGGAGAGA 
GCCAAGCTCG CTAGGAAGGA GAAAGAAAGA AAGCATAGAG AAAAAGAACG TAAAGAGAAT 
GAATATACGA ATAGGAAACA TTTATCTGGT ACCAGAGTTA TCCAAAAGAA TTTAGTGTAC 
GTTGTTGGCA TCAATCCTCC TGTTCCATAC GAGGAAGTTG CGCCCACTCT GAAATCTGAA 
AAATATTTTG GCCAATATGG TAAGATAAAT AAGATTGTGG TTAATAGAAA AACACCCCAT 
TCTAACAACA CAACCAGCGA GCATTATCAC CATCATTCAC CAGGATATGG CGTTTACATA 
ACCTTCGGAT CCAAGGACGA TGCTGCAAGA TGTATAGCTC AGGTAGACGG GACGTATATG 
GATGGCCGCC TGATCAAAGC TGCCTACGGT ACTACTAAAT ACTGTTCTTC TTATTTAAGA 
GGATTGCCAT GCCCAAATCC CAACTGTATG TTTTTGCATG AACCTGGTGA AGAAGCTGAT 
TCTTTTAATA AAAGAGAACT CCACAATAAA CAACAAGCGC AACAGCAAAG TGGCGGAACT 
GCATTCACTA GATCTGGAAT ACACAACAAT ATATCTACCA GTACCGCTGG TTCAAATACC 
AATTTACTAA GTGAAAATTT CACAGGCACA CCTTCACCGG CGGCGATGAG GGCTCAGTTA 
CATCATGACA GCCATACAAA CGCTGGAACA CCGGTATTAA CACCTGCTCC GGTCCCTGCA 
GGGTCAAATC CTTGGGGAGT TACTCAATCA GCAACACCTG TAACCTCTAT CAATCTCTCT 
AAAAACAGCA GCTCCATAAA CTTGCCAACA TTAAATGATT CTCTGGGCCA TCATACTACC 
CCCACAACAG AGAATACCAT CACAAGTACG ACAACTACTA CCAATACCAA TGCTACAAGT 
CACTCCCATG GTAGCAAGAA GAAGCAATCT CTTGCTGCAG AGGAATACAA AGATCCTTAT 
GACGCACTAG GGAATGCTGT TGACTTTTTG GATGCAAGAC TACATTCTCT ATCAAATTAT 
CAGAAGCGCC CTATATCTAT CAAATCCAAT ATTATTGACG AAGAAACTTA TAAAAAGTAT 
CCGTCTTTGT TTTCTTGGGA CAAGATTGAG GCCTCAAAGA AAAGTGACAA TACATTAGCC 
AACAAACTTG TGGAGATCCT GGCTATAAAG CCAATAGACT ACACTGCTTC TGTCGTTCAA 
TTCTTGCAGA GTGTCAATGT TGGTGTAAAT GACAATATTA CAATCACAGA TAATACGAAA 
ACTCCCACCC AACCAATAAG ACTGCAAACC GTCTCACAGC AAATCCAACC ACCATTAAAC 
GTCAGTACCC CTCCACCGGG TATCTTTGGT CCACAACATA AGGTTCCTAT TCAGCAGCAA 
CAAATGGGTG ATACAAGCTC AAGAAATTCC TCTGATTTAC TAAATCAACT AATCAACGGA 
AGGAAAATTA TCGCCGGTAA TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_010991.3
CDS	1..1764
Translation	MMNPHVQENLQAIHNALSNFDTSFLSEDEEDYCPLCIEPMDITDKNFFPCPCGYQICQFCYNNIRQNPELNGRCPACRRKYDDENVRYVTLSPEELKMERAKLARKEKERKHREKERKENEYTNRKHLSGTRVIQKNLVYVVGINPPVPYEEVAPTLKSEKYFGQYGKINKIVVNRKTPHSNNTTSEHYHHHSPGYGVYITFGSKDDAARCIAQVDGTYMDGRLIKAAYGTTKYCSSYLRGLPCPNPNCMFLHEPGEEADSFNKRELHNKQQAQQQSGGTAFTRSGIHNNISTSTAGSNTNLLSENFTGTPSPAAMRAQLHHDSHTNAGTPVLTPAPVPAGSNPWGVTQSATPVTSINLSKNSSSINLPTLNDSLGHHTTPTTENTITSTTTTTNTNATSHSHGSKKKQSLAAEEYKDPYDALGNAVDFLDARLHSLSNYQKRPISIKSNIIDEETYKKYPSLFSWDKIEASKKSDNTLANKLVEILAIKPIDYTASVVQFLQSVNVGVNDNITITDNTKTPTQPIRLQTVSQQIQPPLNVSTPPPGIFGPQHKVPIQQQQMGDTSSRNSSDLLNQLINGRKIIAGN

Target ORF information:

RefSeq Version	NM_001178959.3
Organism	Saccharomyces cerevisiae S288C
Definition	Saccharomyces cerevisiae S288C CCR4-NOT core ubiquitin-protein ligase subunit MOT2 (MOT2), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001178959.3

ORF Insert Sequence:

ATGATGAATC CACACGTTCA AGAAAATTTG CAAGCAATCC ACAACGCCTT AAGCAATTTT 
GATACGTCAT TTTTATCGGA GGATGAAGAA GATTATTGCC CTCTTTGTAT TGAACCAATG 
GATATTACTG ATAAAAATTT TTTTCCTTGT CCCTGTGGTT ATCAAATTTG TCAATTTTGC 
TACAATAATA TCAGACAAAA TCCAGAATTA AATGGCCGTT GCCCAGCATG TCGTCGTAAA 
TATGATGACG AGAACGTCAG ATACGTCACA TTATCTCCGG AGGAGTTAAA AATGGAGAGA 
GCCAAGCTCG CTAGGAAGGA GAAAGAAAGA AAGCATAGAG AAAAAGAACG TAAAGAGAAT 
GAATATACGA ATAGGAAACA TTTATCTGGT ACCAGAGTTA TCCAAAAGAA TTTAGTGTAC 
GTTGTTGGCA TCAATCCTCC TGTTCCATAC GAGGAAGTTG CGCCCACTCT GAAATCTGAA 
AAATATTTTG GCCAATATGG TAAGATAAAT AAGATTGTGG TTAATAGAAA AACACCCCAT 
TCTAACAACA CAACCAGCGA GCATTATCAC CATCATTCAC CAGGATATGG CGTTTACATA 
ACCTTCGGAT CCAAGGACGA TGCTGCAAGA TGTATAGCTC AGGTAGACGG GACGTATATG 
GATGGCCGCC TGATCAAAGC TGCCTACGGT ACTACTAAAT ACTGTTCTTC TTATTTAAGA 
GGATTGCCAT GCCCAAATCC CAACTGTATG TTTTTGCATG AACCTGGTGA AGAAGCTGAT 
TCTTTTAATA AAAGAGAACT CCACAATAAA CAACAAGCGC AACAGCAAAG TGGCGGAACT 
GCATTCACTA GATCTGGAAT ACACAACAAT ATATCTACCA GTACCGCTGG TTCAAATACC 
AATTTACTAA GTGAAAATTT CACAGGCACA CCTTCACCGG CGGCGATGAG GGCTCAGTTA 
CATCATGACA GCCATACAAA CGCTGGAACA CCGGTATTAA CACCTGCTCC GGTCCCTGCA 
GGGTCAAATC CTTGGGGAGT TACTCAATCA GCAACACCTG TAACCTCTAT CAATCTCTCT 
AAAAACAGCA GCTCCATAAA CTTGCCAACA TTAAATGATT CTCTGGGCCA TCATACTACC 
CCCACAACAG AGAATACCAT CACAAGTACG ACAACTACTA CCAATACCAA TGCTACAAGT 
CACTCCCATG GTAGCAAGAA GAAGCAATCT CTTGCTGCAG AGGAATACAA AGATCCTTAT 
GACGCACTAG GGAATGCTGT TGACTTTTTG GATGCAAGAC TACATTCTCT ATCAAATTAT 
CAGAAGCGCC CTATATCTAT CAAATCCAAT ATTATTGACG AAGAAACTTA TAAAAAGTAT 
CCGTCTTTGT TTTCTTGGGA CAAGATTGAG GCCTCAAAGA AAAGTGACAA TACATTAGCC 
AACAAACTTG TGGAGATCCT GGCTATAAAG CCAATAGACT ACACTGCTTC TGTCGTTCAA 
TTCTTGCAGA GTGTCAATGT TGGTGTAAAT GACAATATTA CAATCACAGA TAATACGAAA 
ACTCCCACCC AACCAATAAG ACTGCAAACC GTCTCACAGC AAATCCAACC ACCATTAAAC 
GTCAGTACCC CTCCACCGGG TATCTTTGGT CCACAACATA AGGTTCCTAT TCAGCAGCAA 
CAAATGGGTG ATACAAGCTC AAGAAATTCC TCTGATTTAC TAAATCAACT AATCAACGGA 
AGGAAAATTA TCGCCGGTAA TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The nucleotide sequence of Saccharomyces cerevisiae chromosome V.
Nature387(6632 Suppl)78-81(1997 May)
Dietrich FS,Mulligan J,Hennessy K,Yelton MA,Allen E,Araujo R,Aviles E,Berno A,Brennan T,Carpenter J,Chen E,Cherry JM,Chung E,Duncan M,Guzman E,Hartzell G,Hunicke-Smith S,Hyman RW,Kayser A,Komp C,Lashkari D,Lew H,Lin D,Mosedale D,Davis RW

Life with 6000 genes.
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)
Goffeau A,Barrell BG,Bussey H,Davis RW,Dujon B,Feldmann H,Galibert F,Hoheisel JD,Jacq C,Johnston M,Louis EJ,Mewes HW,Murakami Y,Philippsen P,Tettelin H,Oliver SG

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MOT2 cDNA ORF clone, Saccharomyces cerevisiae S288C

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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