MNL1 cDNA ORF clone, Saccharomyces cerevisiae S288C

Gene
Clones
gRNAs

Summary

Gene Symbol	MNL1
Entrez Gene ID	856611
Full Name	alpha-1,2-mannosidase MNL1
Synonyms	HTM1
Gene Type	protein-coding
Organism	Saccharomyces cerevisiae S288C

mRNA and Protein(s)

mRNA	Protein	Name
NM_001179335.3	NP_012074.3	alpha-1,2-mannosidase MNL1

Pathways from BioSystems

Homologies

Kluyveromyces lactis	KLLA0B03608g	XP_451695.1
Eremothecium gossypii	AGOS_ADL390W	NP_983706.2
Saccharomyces cerevisiae (baker's yeast)	MNL1	NP_012074.3

Gene Ontology
Bibliography

Related articles in PubMed

Life with 6000 genes.
Goffeau A, Barrell BG, Bussey H, Davis RW, Dujon B, Feldmann H, Galibert F, Hoheisel JD, Jacq C, Johnston M, Louis EJ, Mewes HW, Murakami Y, Philippsen P, Tettelin H, Oliver SG
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)

Complete nucleotide sequence of Saccharomyces cerevisiae chromosome VIII.
Johnston M, Andrews S, Brinkman R, Cooper J, Ding H, Dover J, Du Z, Favello A, Fulton L, Gattung S
Science (New York, N.Y.)265(5181)2077-82(1994 Sep)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Data show that binding of mannosidase Htm1 to protein disulfide isomerase Pdi1 is required for endoplasmic reticulum (ER)-associated protein degradation (ERAD).
Title: A Complex of Htm1 and the Oxidoreductase Pdi1 Accelerates Degradation of Misfolded Glycoproteins.

analysis of an Htm1 (EDEM)-dependent, Mns1-independent Endoplasmic Reticulum-associated Degradation (ERAD) pathway in Saccharomyces cerevisiae
Title: Identification of an Htm1 (EDEM)-dependent, Mns1-independent Endoplasmic Reticulum-associated Degradation (ERAD) pathway in Saccharomyces cerevisiae: application of a novel assay for glycoprotein ERAD.

Systematic analysis of model substrates revealed that glycans mark structural determinants that are sensitive to the overall folding state of the molecule.
Title: Intrinsic conformational determinants signal protein misfolding to the Hrd1/Htm1 endoplasmic reticulum-associated degradation system.

This disulfide bond is essential for the ERAD activity of Mnl1p and in turn stabilizes the prolonged association of PDI with Mnl1p
Title: Roles of protein-disulfide isomerase-mediated disulfide bond formation of yeast Mnl1p in endoplasmic reticulum-associated degradation.

Htm1 protein is an alpha1,2-specific exomannosidase that generates the Man(7)GlcNAc(2) oligosaccharide with a terminal alpha1,6-linked mannosyl residue on degradation substrates.
Title: Htm1 protein generates the N-glycan signal for glycoprotein degradation in the endoplasmic reticulum.

The following MNL1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the MNL1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OSi05399	NM_001179335.3 Latest version!	Saccharomyces cerevisiae S288C alpha-1,2-mannosidase MNL1 (MNL1), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

MNL1 ( NM_001179335.3 ) cDNA ORF clone, Saccharomyces cerevisiae S288C -> NP_012074.3 Saccharomyces cerevisiae S288C alpha-1,2-mannosidase MNL1 (MNL1), partial mRNA.

Price & Availability↑

CloneID	OSi05399
Clone ID Related Accession (Same CDS sequence)	NM_001179335.3
Accession Version	NM_001179335.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2391bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-31
Organism	Saccharomyces cerevisiae S288C
Product	alpha-1,2-mannosidase MNL1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by SGD. This record is derived from an annotated genomic sequence (NC_001140). On Jul 30, 2012 this sequence version replaced NM_001179335.2. ##Genome-Annotation-Data-START## Annotation Provider :: SGD Annotation Status :: Full Annotation Annotation Veresion :: R64-2-1 URL :: http://www.yeastgenome.org/ ##Genome-Annotation-Data-END## COMPLETENESS: incomplete on both ends.

ATGGTTTGCT GCTTATGGGT GCTTCTAGCA TTGTTGCTTC ATCTCGATCA TGTAGCATGC 
GAAGACGATG CGTACTCATT CACTTCTAAA GAACTTAAGG CTTACAAGCA GGAAGTGAAG 
GAGCTGTTTT ATTTCGGCTT CGACAATTAT TTAGAGCATG GGTATCCATA TGATGAGGTA 
AAACCCATTT CATGCGTTCC TAAGAAGAGA AACTTTGAAG ATCCTACTGA TCAAGGCACC 
AACGATATAT TGGGCAATTT TACAATCACT TTAATAGATT CTTTAACCAC TATTGCTATA 
CTGGAAGACC GGCCGCAGTT TTTGAAAGCT GTTCGACTTG TTGAAAGGAC ATTTCCTGAT 
GGCAATTTCG ATATTGATTC CACAATACAA GTTTTTGAAA TTACTATACG TGTTATCGGA 
TCACTACTAT CATCACATCT TTATGCTACG GATCCCACAA AGGCAGTGTA TTTAGGGGAC 
GATTATGATG GCTCACTGTT ACGTCTCGCC CAGAATATGG CAGATAGGCT CTTACCTGCA 
TATTTAACGT CTACCGGATT ACCTATGCCA CGGAGAAACA TCAAACGAAA ATGGGATGTC 
TCTGAATTTC CAGAATTTTT GGAGACAGAG AATAATGTGG CCGCTATGGC GTCTCCCATG 
TTCGAATTCA CTATTTTATC TTACTTGACA GGCGATCCCA AGTATGAGAA AGTTACCAGA 
TACGCATTTG ATAAAACATG GTCTCTTAGA ACTGGTTTGG ATTTGCTACC CATGTCCTTT 
CACCCCGAAA AACTAACACC ATATACGCCC ATGACTGGAA TCGGCGCCTC CATCGACTCC 
CTTTTCGAAT ACGCTTTGAA AGGTGCTATT TTATTTGATG ATTCAGAATT AATGGAAGTT 
TGGAACGTAG CATACGAGGC ATTAAAGACT AACTGTAAAA ACGATTGGTT TTTTGCTAAT 
GTAATGGCTG ACACAGGGCA TTTGTTTGTT CCCTGGATTG ATTCTTTGAG TGCTTTTTTC 
TCCGGTTTAC AGGTGCTTGC TGGTGATTTG GATGATGCCA TAGCGAACCA TTTAATGTTT 
TTAAAAATGT GGAACACCTT TGGCGGTATT CCTGAAAGGT GGAACTTTAG TCCTCCGGAG 
TTTCCCCCAC TATCTCCATT AGAGCGATCG GGAGCTGTTG CTTTGGATAA TATCCTCCCC 
TTAGAATGGT ACCCATTAAG ACCGGAATTT TTTGAGTCAA CCTATTTCTT ATATAGAGCT 
ACAAAAGACC CGTTTTACTT AAATATTGGG GTACATCTGC TAAAAGACCT CAAGCAACGA 
TTTAAGTCTA ACTGCGGATT TGCAGGTTTC CAAAATGTAA TCACGGGCGA ACTGCAAGAT 
AGAATGGAAA CATTTGTGTT GAGTGAAACT TTGAAATACC TGTATTTATT ATTTGATGAA 
GAGAACGAAT TACATAACAG CGCTAGTGAC GTAATTTTCA GTACAGAAGC CCATCCAATG 
TGGTTGCCCC AAGAAGTGAG GTCCAACTAT AAACGTAACG CTAAATTTAA CAACTCTGTC 
TACTCGTCGC ACTTGGAAAT TTGCCAGAAA AAGGACAGGG AACAAGCTGG AGAGAACACA 
TTGAGCCAAA GAATTGTGGG GTTCGCCAAA TCGATCTTTC ATAAAGGTCC ACCAGACGAA 
GAGGCTACTG ATCCAATTAT AGACTATACC ATTGATACTG AACTTCCCGG TACATGCTCT 
ATAAAGCCTC ATCATGTAAT TGGTGACGAA TTTTGGTATT CTCCAATGTT GAGCAATTTT 
GACAGATTAT TCGAGATTGA CTCCCGGTTC GCTGCCACGC TAATTAAACC TTCACACATG 
CATAACTATA ATGCAATTGA ACTAGAACCT GGATTTTATA ACAGGTGGTC CAATCCGCAA 
TTTAGCACGT GCCTGATCCC ACCCACCACC GAAATTTTCG AACTACTATT CGACCTTCCT 
GGATATCATC AGTTGAATCC ATTAATGTTA GAAAACAAAA CTATTACATT TGAAACCTTT 
GGAGGTAGGT CAAGGTTGAA GATCGAAAAA TTGCAAATCT ACCAAATTGA TTACTATGGA 
GACTTAATCA CTGCATCGAC ATTCCAAGAC GTCTCCCGCA AGGATATTTT CTCGAATGCA 
TGCGATGCAG TGGCAAGCTT GTATTCTCCC ACTTATCTTT ACAGAGTAGT AGCCATCAAC 
GGACGCATAT TGCCTCGCCA TGGAAGTGTA CAAATCAAGA AACATAGTCC TGTGCTCACT 
TCCAATGGCA CAAGAGAGGA AGACGAATTC AAAATGGATG GCATCGGGAT TAATGACCAT 
TCCCAACTCA TGTTAGAATG CACGCCCATC ATCAACTTAT TTATTGTATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_012074.3
CDS	1..2391
Translation	MVCCLWVLLALLLHLDHVACEDDAYSFTSKELKAYKQEVKELFYFGFDNYLEHGYPYDEVKPISCVPKKRNFEDPTDQGTNDILGNFTITLIDSLTTIAILEDRPQFLKAVRLVERTFPDGNFDIDSTIQVFEITIRVIGSLLSSHLYATDPTKAVYLGDDYDGSLLRLAQNMADRLLPAYLTSTGLPMPRRNIKRKWDVSEFPEFLETENNVAAMASPMFEFTILSYLTGDPKYEKVTRYAFDKTWSLRTGLDLLPMSFHPEKLTPYTPMTGIGASIDSLFEYALKGAILFDDSELMEVWNVAYEALKTNCKNDWFFANVMADTGHLFVPWIDSLSAFFSGLQVLAGDLDDAIANHLMFLKMWNTFGGIPERWNFSPPEFPPLSPLERSGAVALDNILPLEWYPLRPEFFESTYFLYRATKDPFYLNIGVHLLKDLKQRFKSNCGFAGFQNVITGELQDRMETFVLSETLKYLYLLFDEENELHNSASDVIFSTEAHPMWLPQEVRSNYKRNAKFNNSVYSSHLEICQKKDREQAGENTLSQRIVGFAKSIFHKGPPDEEATDPIIDYTIDTELPGTCSIKPHHVIGDEFWYSPMLSNFDRLFEIDSRFAATLIKPSHMHNYNAIELEPGFYNRWSNPQFSTCLIPPTTEIFELLFDLPGYHQLNPLMLENKTITFETFGGRSRLKIEKLQIYQIDYYGDLITASTFQDVSRKDIFSNACDAVASLYSPTYLYRVVAINGRILPRHGSVQIKKHSPVLTSNGTREEDEFKMDGIGINDHSQLMLECTPIINLFIV

Target ORF information:

RefSeq Version	NM_001179335.3
Organism	Saccharomyces cerevisiae S288C
Definition	Saccharomyces cerevisiae S288C alpha-1,2-mannosidase MNL1 (MNL1), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001179335.3

ORF Insert Sequence:

ATGGTTTGCT GCTTATGGGT GCTTCTAGCA TTGTTGCTTC ATCTCGATCA TGTAGCATGC 
GAAGACGATG CGTACTCATT CACTTCTAAA GAACTTAAGG CTTACAAGCA GGAAGTGAAG 
GAGCTGTTTT ATTTCGGCTT CGACAATTAT TTAGAGCATG GGTATCCATA TGATGAGGTA 
AAACCCATTT CATGCGTTCC TAAGAAGAGA AACTTTGAAG ATCCTACTGA TCAAGGCACC 
AACGATATAT TGGGCAATTT TACAATCACT TTAATAGATT CTTTAACCAC TATTGCTATA 
CTGGAAGACC GGCCGCAGTT TTTGAAAGCT GTTCGACTTG TTGAAAGGAC ATTTCCTGAT 
GGCAATTTCG ATATTGATTC CACAATACAA GTTTTTGAAA TTACTATACG TGTTATCGGA 
TCACTACTAT CATCACATCT TTATGCTACG GATCCCACAA AGGCAGTGTA TTTAGGGGAC 
GATTATGATG GCTCACTGTT ACGTCTCGCC CAGAATATGG CAGATAGGCT CTTACCTGCA 
TATTTAACGT CTACCGGATT ACCTATGCCA CGGAGAAACA TCAAACGAAA ATGGGATGTC 
TCTGAATTTC CAGAATTTTT GGAGACAGAG AATAATGTGG CCGCTATGGC GTCTCCCATG 
TTCGAATTCA CTATTTTATC TTACTTGACA GGCGATCCCA AGTATGAGAA AGTTACCAGA 
TACGCATTTG ATAAAACATG GTCTCTTAGA ACTGGTTTGG ATTTGCTACC CATGTCCTTT 
CACCCCGAAA AACTAACACC ATATACGCCC ATGACTGGAA TCGGCGCCTC CATCGACTCC 
CTTTTCGAAT ACGCTTTGAA AGGTGCTATT TTATTTGATG ATTCAGAATT AATGGAAGTT 
TGGAACGTAG CATACGAGGC ATTAAAGACT AACTGTAAAA ACGATTGGTT TTTTGCTAAT 
GTAATGGCTG ACACAGGGCA TTTGTTTGTT CCCTGGATTG ATTCTTTGAG TGCTTTTTTC 
TCCGGTTTAC AGGTGCTTGC TGGTGATTTG GATGATGCCA TAGCGAACCA TTTAATGTTT 
TTAAAAATGT GGAACACCTT TGGCGGTATT CCTGAAAGGT GGAACTTTAG TCCTCCGGAG 
TTTCCCCCAC TATCTCCATT AGAGCGATCG GGAGCTGTTG CTTTGGATAA TATCCTCCCC 
TTAGAATGGT ACCCATTAAG ACCGGAATTT TTTGAGTCAA CCTATTTCTT ATATAGAGCT 
ACAAAAGACC CGTTTTACTT AAATATTGGG GTACATCTGC TAAAAGACCT CAAGCAACGA 
TTTAAGTCTA ACTGCGGATT TGCAGGTTTC CAAAATGTAA TCACGGGCGA ACTGCAAGAT 
AGAATGGAAA CATTTGTGTT GAGTGAAACT TTGAAATACC TGTATTTATT ATTTGATGAA 
GAGAACGAAT TACATAACAG CGCTAGTGAC GTAATTTTCA GTACAGAAGC CCATCCAATG 
TGGTTGCCCC AAGAAGTGAG GTCCAACTAT AAACGTAACG CTAAATTTAA CAACTCTGTC 
TACTCGTCGC ACTTGGAAAT TTGCCAGAAA AAGGACAGGG AACAAGCTGG AGAGAACACA 
TTGAGCCAAA GAATTGTGGG GTTCGCCAAA TCGATCTTTC ATAAAGGTCC ACCAGACGAA 
GAGGCTACTG ATCCAATTAT AGACTATACC ATTGATACTG AACTTCCCGG TACATGCTCT 
ATAAAGCCTC ATCATGTAAT TGGTGACGAA TTTTGGTATT CTCCAATGTT GAGCAATTTT 
GACAGATTAT TCGAGATTGA CTCCCGGTTC GCTGCCACGC TAATTAAACC TTCACACATG 
CATAACTATA ATGCAATTGA ACTAGAACCT GGATTTTATA ACAGGTGGTC CAATCCGCAA 
TTTAGCACGT GCCTGATCCC ACCCACCACC GAAATTTTCG AACTACTATT CGACCTTCCT 
GGATATCATC AGTTGAATCC ATTAATGTTA GAAAACAAAA CTATTACATT TGAAACCTTT 
GGAGGTAGGT CAAGGTTGAA GATCGAAAAA TTGCAAATCT ACCAAATTGA TTACTATGGA 
GACTTAATCA CTGCATCGAC ATTCCAAGAC GTCTCCCGCA AGGATATTTT CTCGAATGCA 
TGCGATGCAG TGGCAAGCTT GTATTCTCCC ACTTATCTTT ACAGAGTAGT AGCCATCAAC 
GGACGCATAT TGCCTCGCCA TGGAAGTGTA CAAATCAAGA AACATAGTCC TGTGCTCACT 
TCCAATGGCA CAAGAGAGGA AGACGAATTC AAAATGGATG GCATCGGGAT TAATGACCAT 
TCCCAACTCA TGTTAGAATG CACGCCCATC ATCAACTTAT TTATTGTATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Life with 6000 genes.
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)
Goffeau A,Barrell BG,Bussey H,Davis RW,Dujon B,Feldmann H,Galibert F,Hoheisel JD,Jacq C,Johnston M,Louis EJ,Mewes HW,Murakami Y,Philippsen P,Tettelin H,Oliver SG

Complete nucleotide sequence of Saccharomyces cerevisiae chromosome VIII.
Science (New York, N.Y.)265(5181)2077-82(1994 Sep)
Johnston M,Andrews S,Brinkman R,Cooper J,Ding H,Dover J,Du Z,Favello A,Fulton L,Gattung S

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MNL1 cDNA ORF clone, Saccharomyces cerevisiae S288C

Gene

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gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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