DNA2 cDNA ORF clone, Saccharomyces cerevisiae S288C

Gene
Clones
gRNAs

Summary

Gene Symbol	DNA2
Entrez Gene ID	856569
Full Name	bifunctional ATP-dependent DNA helicase/ssDNA endodeoxyribonuclease DNA2
Synonyms	WEB2
Gene Type	protein-coding
Organism	Saccharomyces cerevisiae S288C

mRNA and Protein(s)

mRNA	Protein	Name
NM_001179295.1	NP_012034.1	bifunctional ATP-dependent DNA helicase/ssDNA endodeoxyribonuclease DNA2

Pathways from BioSystems

Homologies

Saccharomyces cerevisiae (baker's yeast)	DNA2	NP_012034.1
Kluyveromyces lactis	KLLA0A05324g	XP_451235.1
Eremothecium gossypii	AGOS_AEL218W	NP_984643.1

Gene Ontology
Bibliography

Related articles in PubMed

Life with 6000 genes.
Goffeau A, Barrell BG, Bussey H, Davis RW, Dujon B, Feldmann H, Galibert F, Hoheisel JD, Jacq C, Johnston M, Louis EJ, Mewes HW, Murakami Y, Philippsen P, Tettelin H, Oliver SG
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)

Complete nucleotide sequence of Saccharomyces cerevisiae chromosome VIII.
Johnston M, Andrews S, Brinkman R, Cooper J, Ding H, Dover J, Du Z, Favello A, Fulton L, Gattung S
Science (New York, N.Y.)265(5181)2077-82(1994 Sep)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

We also present evidence that indicates Rim11/Ume6 works independently but in parallel with that of checkpoint inhibition, dNTP regulation, and sister-chromatid recombination. In conclusion, our results establish Rim11, Ume6, the histone deacetylase complex Sin3-Rpd3 and Sml1 as new factors important in the events of faulty lagging strand synthesis.
Title: GSK-3? Homolog Rim11 and the Histone Deacetylase Complex Ume6-Sin3-Rpd3 Are Involved in Replication Stress Response Caused by Defects in Dna2.

role of the Dna2 translocase activity in DNA break end resection and in the imposition of the 5' strand specificity of end resection.
Title: A novel role of the Dna2 translocase function in DNA break resection.

Ctf4-dependent recruitment of Dna2/Tof2 couples other processes to DNA synthesis, including rDNA copy-number regulation.
Title: Ctf4 Is a Hub in the Eukaryotic Replisome that Links Multiple CIP-Box Proteins to the CMG Helicase.

A model is proposed where Dna2 alone is responsible for cleaving of RPA-bound long DNA flaps.
Title: The Saccharomyces cerevisiae Dna2 can function as a sole nuclease in the processing of Okazaki fragments in DNA replication.

Data show that Dna2 is required for normal telomeric CA-strand fill-in.
Title: Dna2 is involved in CA strand resection and nascent lagging strand completion at native yeast telomeres.

The following DNA2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the DNA2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OSi05361	NM_001179295.1 Latest version!	Saccharomyces cerevisiae S288C bifunctional ATP-dependent DNA helicase/ssDNA endodeoxyribonuclease DNA2 (DNA2), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

DNA2 ( NM_001179295.1 ) cDNA ORF clone, Saccharomyces cerevisiae S288C -> NP_012034.1 Saccharomyces cerevisiae S288C bifunctional ATP-dependent DNA helicase/ssDNA endodeoxyribonuclease DNA2 (DNA2), partial mRNA.

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CloneID	OSi05361
Clone ID Related Accession (Same CDS sequence)	NM_001179295.1
Accession Version	NM_001179295.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4569bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-31
Organism	Saccharomyces cerevisiae S288C
Product	bifunctional ATP-dependent DNA helicase/ssDNA endodeoxyribonuclease DNA2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by SGD. This record is derived from an annotated genomic sequence (NC_001140). ##Genome-Annotation-Data-START## Annotation Provider :: SGD Annotation Status :: Full Annotation Annotation Veresion :: R64-2-1 URL :: http://www.yeastgenome.org/ ##Genome-Annotation-Data-END## COMPLETENESS: incomplete on both ends.

ATGCCCGGAA CGCCACAGAA GAACAAGAGG TCTGCGAGTA TATCTGTTTC ACCTGCGAAG 
AAGACAGAGG AAAAAGAAAT AATACAAAAT GATTCAAAGG CGATATTATC TAAGCAAACT 
AAGAGGAAAA AAAAGTATGC TTTTGCTCCT ATAAATAACT TAAATGGGAA GAACACCAAG 
GTATCCAATG CAAGTGTCCT AAAGTCAATA GCCGTTTCAC AAGTACGAAA TACATCAAGA 
ACAAAAGATA TAAACAAAGC AGTTAGCAAG AGCGTAAAGC AATTACCAAA TTCACAAGTT 
AAACCGAAAC GGGAAATGTC TAATTTGAGT AGGCATCACG ATTTCACTCA AGACGAGGAC 
GGCCCCATGG AAGAAGTAAT ATGGAAATAT TCACCTTTAC AACGAGATAT GTCCGACAAA 
ACAACAAGCG CTGCCGAATA CTCTGATGAC TACGAAGATG TCCAAAATCC CTCTTCTACA 
CCTATAGTCC CTAATCGACT GAAAACTGTT CTTAGTTTCA CAAATATCCA AGTACCCAAC 
GCTGACGTAA ATCAACTCAT TCAAGAAAAT GGAAATGAGC AAGTGCGTCC TAAACCAGCA 
GAAATATCTA CCAGGGAGTC TCTCCGTAAT ATAGATGATA TTCTCGATGA TATAGAGGGC 
GATTTAACTA TAAAACCGAC GATAACGAAA TTCAGCGATT TGCCATCTTC ACCCATCAAG 
GCACCCAACG TTGAAAAAAA AGCAGAGGTG AATGCAGAAG AAGTAGATAA AATGGATTCA 
ACAGGAGATA GCAACGATGG CGATGACTCT TTGATAGATA TTTTAACTCA AAAGTATGTT 
GAGAAACGCA AGAGTGAGAG TCAGATAACA ATTCAAGGCA ACACCAATCA AAAAAGTGGA 
GCCCAGGAAA GTTGTGGGAA GAATGATAAC ACAAAATCGA GAGGAGAAAT TGAAGATCAT 
GAAAATGTAG ACAATCAAGC CAAAACAGGC AATGCATTTT ATGAGAATGA AGAAGACAGT 
AATTGCCAAA GAATTAAAAA AAATGAAAAA ATAGAATACA ATTCTTCAGA CGAATTTTCA 
GACGATTCAC TAATTGAACT ATTGAACGAA ACTCAAACAC AAGTGGAACC TAACACTATT 
GAACAAGATT TAGACAAAGT GGAGAAAATG GTCTCTGACG ATTTGAGAAT TGCTACTGAT 
TCCACTTTAT CCGCATATGC TCTCAGAGCT AAGTCTGGTG CTCCAAGAGA CGGCGTAGTA 
CGATTAGTAA TAGTAAGCTT ACGTAGTGTT GAATTGCCAA AAATTGGAAC GCAAAAAATA 
CTAGAGTGTA TAGACGGCAA AGGCGAACAA AGTTCTGTTG TCGTTCGACA TCCTTGGGTA 
TATTTGGAAT TTGAGGTTGG GGATGTAATA CATATTATAG AAGGGAAAAA TATTGAAAAC 
AAACGGCTTC TTTCTGACGA CAAAAACCCT AAAACTCAAC TGGCTAACGA CAACCTGTTG 
GTGCTAAACC CTGATGTATT ATTTTCGGCA ACATCAGTCG GAAGTTCAGT AGGTTGTTTA 
AGACGTTCAA TTCTGCAAAT GCAATTTCAA GATCCCCGCG GAGAACCAAG CCTTGTCATG 
ACTTTAGGCA ATATCGTACA CGAGTTATTG CAAGACTCAA TCAAATACAA GCTCTCGCAT 
AATAAAATTT CTATGGAAAT CATAATACAA AAGCTGGATT CTTTGTTAGA AACTTATTCT 
TTTTCAATTA TCATTTGTAA TGAAGAAATC CAGTACGTTA AAGAATTAGT TATGAAAGAA 
CATGCTGAAA ACATTTTGTA CTTCGTCAAT AAATTTGTTT CGAAAAGTAA CTATGGTTGC 
TACACATCAA TTTCCGGAAC AAGAAGAACA CAGCCAATTT CTATATCCAA TGTGATTGAT 
ATTGAAGAAA ACATTTGGTC TCCAATATAT GGTCTTAAAG GGTTCCTAGA TGCAACTGTT 
GAAGCTAATG TTGAAAACAA TAAAAAACAC ATCGTTCCTT TAGAAGTGAA AACTGGAAAA 
TCCAGAAGCG TTTCATACGA GGTTCAAGGC CTTATTTATA CACTTCTGTT AAATGATCGC 
TATGAGATAC CGATAGAGTT TTTTCTTCTT TATTTTACGA GGGATAAAAA TATGACAAAG 
TTCCCTTCTG TTCTGCACTC CATTAAACAT ATTCTCATGT CCCGAAATAG AATGAGTATG 
AATTTTAAGC ATCAACTCCA AGAGGTTTTC GGACAGGCTC AATCAAGGTT TGAATTACCA 
CCCTTACTGC GCGATTCATC TTGTGATTCA TGTTTCATCA AAGAATCATG CATGGTGTTG 
AATAAGCTAC TAGAGGACGG GACACCAGAA GAAAGTGGCC TGGTAGAAGG TGAGTTTGAA 
ATTTTGACAA ACCATTTGTC GCAAAATCTC GCCAACTACA AAGAGTTTTT TACAAAATAT 
AATGATCTAA TAACTAAGGA GGAGTCAAGT ATAACATGTG TAAATAAAGA ACTTTTCTTA 
TTGGATGGTT CCACAAGAGA ATCCAGAAGT GGAAGGTGCC TTTCTGGCTT GGTTGTGTCA 
GAGGTGGTTG AACATGAAAA AACAGAGGGA GCGTATATAT ATTGCTTCTC AAGACGTAGA 
AATGATAATA ACTCGCAGTC GATGTTATCA TCTCAAATTG CTGCTAATGA CTTTGTTATT 
ATTAGTGATG AGGAGGGTCA TTTTTGCTTG TGCCAAGGTC GTGTACAATT TATAAATCCC 
GCTAAAATTG GTATCTCCGT AAAACGAAAG TTATTAAACA ATAGGCTTCT AGATAAGGAA 
AAAGGAGTCA CTACCATCCA AAGTGTGGTC GAATCAGAGC TAGAACAATC CAGTCTCATA 
GCTACCCAAA ATTTGGTCAC ATATAGAATC GACAAAAATG ATATCCAACA GAGTTTGTCT 
TTAGCGCGAT TTAATCTTTT AAGCTTGTTT TTACCAGCTG TTTCACCTGG CGTGGATATT 
GTTGACGAAA GGAGTAAATT ATGTCGGAAG ACAAAGAGAT CTGATGGAGG TAATGAAATA 
CTGCGATCAC TTTTAGTTGA TAATAGAGCA CCTAAGTTTA GAGATGCAAA TGATGATCCC 
GTCATTCCGT ATAAGCTTTC AAAGGATACA ACATTGAACT TGAATCAAAA AGAAGCAATA 
GACAAAGTAA TGAGGGCCGA AGATTATGCA TTGATATTGG GAATGCCAGG GACTGGTAAG 
ACTACTGTTA TCGCAGAAAT TATTAAAATT CTCGTTTCCG AGGGGAAACG CGTGCTTTTG 
ACATCATACA CACATTCAGC AGTGGATAAT ATTTTAATAA AATTGAGGAA CACCAACATT 
AGCATAATGA GATTGGGAAT GAAACATAAG GTCCATCCTG ACACGCAAAA ATATGTCCCG 
AATTACGCGT CAGTGAAAAG TTATAACGAT TATTTGTCCA AAATTAACAG CACCTCTGTT 
GTTGCTACCA CCTGCTTAGG AATTAACGAT ATACTATTTA CACTAAATGA AAAGGATTTT 
GATTATGTGA TTTTGGACGA GGCAAGTCAA ATTTCAATGC CTGTCGCTTT GGGACCTCTG 
CGGTATGGAA ATAGGTTTAT TATGGTAGGA GATCATTACC AGCTACCGCC ATTAGTTAAA 
AATGACGCAG CTCGATTAGG TGGGTTAGAA GAGTCACTGT TCAAGACTTT CTGTGAAAAG 
CATCCGGAAA GCGTTGCAGA ACTGACTTTA CAGTACCGTA TGTGCGGCGA TATTGTAACT 
TTGTCTAATT TCCTAATTTA TGATAATAAA TTAAAATGCG GGAATAACGA AGTTTTTGCT 
CAATCTTTAG AGTTACCAAT GCCTGAGGCC TTGTCGCGCT ATAGGAATGA ATCGGCTAAC 
TCTAAGCAGT GGCTTGAGGA TATACTAGAG CCTACAAGAA AAGTGGTATT TTTAAATTAC 
GATAACTGCC CGGATATTAT CGAACAAAGC GAAAAGGATA ACATTACGAA TCACGGGGAG 
GCAGAATTGA CTCTACAATG TGTGGAAGGA ATGTTATTGA GCGGCGTACC ATGCGAAGAT 
ATTGGTGTAA TGACGTTATA CAGAGCACAG TTAAGATTAC TGAAGAAGAT ATTCAACAAA 
AATGTGTATG ACGGGCTAGA GATCTTGACT GCTGATCAGT TTCAAGGTCG TGACAAAAAG 
TGCATTATTA TTTCCATGGT TAGAAGAAAT TCTCAATTAA ATGGAGGAGC GCTGCTGAAA 
GAGCTAAGAA GAGTCAATGT TGCCATGACA AGGGCCAAAT CCAAGCTGAT CATTATAGGT 
TCAAAAAGTA CAATCGGCTC TGTTCCTGAA ATAAAATCGT TCGTGAACCT GTTAGAAGAG 
CGTAATTGGG TCTATACAAT GTGCAAGGAT GCACTCTACA AATACAAATT TCCCGACAGA 
TCAAACGCTA TTGATGAGGC ACGCAAAGGT TGTGGAAAAA GAACAGGTGC CAAACCCATC 
ACATCAAAGT CTAAATTCGT AAGCGATAAA CCTATCATAA AGGAAATTCT ACAAGAGTAT 
GAAAGTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_012034.1
CDS	1..4569
Translation	MPGTPQKNKRSASISVSPAKKTEEKEIIQNDSKAILSKQTKRKKKYAFAPINNLNGKNTKVSNASVLKSIAVSQVRNTSRTKDINKAVSKSVKQLPNSQVKPKREMSNLSRHHDFTQDEDGPMEEVIWKYSPLQRDMSDKTTSAAEYSDDYEDVQNPSSTPIVPNRLKTVLSFTNIQVPNADVNQLIQENGNEQVRPKPAEISTRESLRNIDDILDDIEGDLTIKPTITKFSDLPSSPIKAPNVEKKAEVNAEEVDKMDSTGDSNDGDDSLIDILTQKYVEKRKSESQITIQGNTNQKSGAQESCGKNDNTKSRGEIEDHENVDNQAKTGNAFYENEEDSNCQRIKKNEKIEYNSSDEFSDDSLIELLNETQTQVEPNTIEQDLDKVEKMVSDDLRIATDSTLSAYALRAKSGAPRDGVVRLVIVSLRSVELPKIGTQKILECIDGKGEQSSVVVRHPWVYLEFEVGDVIHIIEGKNIENKRLLSDDKNPKTQLANDNLLVLNPDVLFSATSVGSSVGCLRRSILQMQFQDPRGEPSLVMTLGNIVHELLQDSIKYKLSHNKISMEIIIQKLDSLLETYSFSIIICNEEIQYVKELVMKEHAENILYFVNKFVSKSNYGCYTSISGTRRTQPISISNVIDIEENIWSPIYGLKGFLDATVEANVENNKKHIVPLEVKTGKSRSVSYEVQGLIYTLLLNDRYEIPIEFFLLYFTRDKNMTKFPSVLHSIKHILMSRNRMSMNFKHQLQEVFGQAQSRFELPPLLRDSSCDSCFIKESCMVLNKLLEDGTPEESGLVEGEFEILTNHLSQNLANYKEFFTKYNDLITKEESSITCVNKELFLLDGSTRESRSGRCLSGLVVSEVVEHEKTEGAYIYCFSRRRNDNNSQSMLSSQIAANDFVIISDEEGHFCLCQGRVQFINPAKIGISVKRKLLNNRLLDKEKGVTTIQSVVESELEQSSLIATQNLVTYRIDKNDIQQSLSLARFNLLSLFLPAVSPGVDIVDERSKLCRKTKRSDGGNEILRSLLVDNRAPKFRDANDDPVIPYKLSKDTTLNLNQKEAIDKVMRAEDYALILGMPGTGKTTVIAEIIKILVSEGKRVLLTSYTHSAVDNILIKLRNTNISIMRLGMKHKVHPDTQKYVPNYASVKSYNDYLSKINSTSVVATTCLGINDILFTLNEKDFDYVILDEASQISMPVALGPLRYGNRFIMVGDHYQLPPLVKNDAARLGGLEESLFKTFCEKHPESVAELTLQYRMCGDIVTLSNFLIYDNKLKCGNNEVFAQSLELPMPEALSRYRNESANSKQWLEDILEPTRKVVFLNYDNCPDIIEQSEKDNITNHGEAELTLQCVEGMLLSGVPCEDIGVMTLYRAQLRLLKKIFNKNVYDGLEILTADQFQGRDKKCIIISMVRRNSQLNGGALLKELRRVNVAMTRAKSKLIIIGSKSTIGSVPEIKSFVNLLEERNWVYTMCKDALYKYKFPDRSNAIDEARKGCGKRTGAKPITSKSKFVSDKPIIKEILQEYES

Target ORF information:

RefSeq Version	NM_001179295.1
Organism	Saccharomyces cerevisiae S288C
Definition	Saccharomyces cerevisiae S288C bifunctional ATP-dependent DNA helicase/ssDNA endodeoxyribonuclease DNA2 (DNA2), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001179295.1

ORF Insert Sequence:

ATGCCCGGAA CGCCACAGAA GAACAAGAGG TCTGCGAGTA TATCTGTTTC ACCTGCGAAG 
AAGACAGAGG AAAAAGAAAT AATACAAAAT GATTCAAAGG CGATATTATC TAAGCAAACT 
AAGAGGAAAA AAAAGTATGC TTTTGCTCCT ATAAATAACT TAAATGGGAA GAACACCAAG 
GTATCCAATG CAAGTGTCCT AAAGTCAATA GCCGTTTCAC AAGTACGAAA TACATCAAGA 
ACAAAAGATA TAAACAAAGC AGTTAGCAAG AGCGTAAAGC AATTACCAAA TTCACAAGTT 
AAACCGAAAC GGGAAATGTC TAATTTGAGT AGGCATCACG ATTTCACTCA AGACGAGGAC 
GGCCCCATGG AAGAAGTAAT ATGGAAATAT TCACCTTTAC AACGAGATAT GTCCGACAAA 
ACAACAAGCG CTGCCGAATA CTCTGATGAC TACGAAGATG TCCAAAATCC CTCTTCTACA 
CCTATAGTCC CTAATCGACT GAAAACTGTT CTTAGTTTCA CAAATATCCA AGTACCCAAC 
GCTGACGTAA ATCAACTCAT TCAAGAAAAT GGAAATGAGC AAGTGCGTCC TAAACCAGCA 
GAAATATCTA CCAGGGAGTC TCTCCGTAAT ATAGATGATA TTCTCGATGA TATAGAGGGC 
GATTTAACTA TAAAACCGAC GATAACGAAA TTCAGCGATT TGCCATCTTC ACCCATCAAG 
GCACCCAACG TTGAAAAAAA AGCAGAGGTG AATGCAGAAG AAGTAGATAA AATGGATTCA 
ACAGGAGATA GCAACGATGG CGATGACTCT TTGATAGATA TTTTAACTCA AAAGTATGTT 
GAGAAACGCA AGAGTGAGAG TCAGATAACA ATTCAAGGCA ACACCAATCA AAAAAGTGGA 
GCCCAGGAAA GTTGTGGGAA GAATGATAAC ACAAAATCGA GAGGAGAAAT TGAAGATCAT 
GAAAATGTAG ACAATCAAGC CAAAACAGGC AATGCATTTT ATGAGAATGA AGAAGACAGT 
AATTGCCAAA GAATTAAAAA AAATGAAAAA ATAGAATACA ATTCTTCAGA CGAATTTTCA 
GACGATTCAC TAATTGAACT ATTGAACGAA ACTCAAACAC AAGTGGAACC TAACACTATT 
GAACAAGATT TAGACAAAGT GGAGAAAATG GTCTCTGACG ATTTGAGAAT TGCTACTGAT 
TCCACTTTAT CCGCATATGC TCTCAGAGCT AAGTCTGGTG CTCCAAGAGA CGGCGTAGTA 
CGATTAGTAA TAGTAAGCTT ACGTAGTGTT GAATTGCCAA AAATTGGAAC GCAAAAAATA 
CTAGAGTGTA TAGACGGCAA AGGCGAACAA AGTTCTGTTG TCGTTCGACA TCCTTGGGTA 
TATTTGGAAT TTGAGGTTGG GGATGTAATA CATATTATAG AAGGGAAAAA TATTGAAAAC 
AAACGGCTTC TTTCTGACGA CAAAAACCCT AAAACTCAAC TGGCTAACGA CAACCTGTTG 
GTGCTAAACC CTGATGTATT ATTTTCGGCA ACATCAGTCG GAAGTTCAGT AGGTTGTTTA 
AGACGTTCAA TTCTGCAAAT GCAATTTCAA GATCCCCGCG GAGAACCAAG CCTTGTCATG 
ACTTTAGGCA ATATCGTACA CGAGTTATTG CAAGACTCAA TCAAATACAA GCTCTCGCAT 
AATAAAATTT CTATGGAAAT CATAATACAA AAGCTGGATT CTTTGTTAGA AACTTATTCT 
TTTTCAATTA TCATTTGTAA TGAAGAAATC CAGTACGTTA AAGAATTAGT TATGAAAGAA 
CATGCTGAAA ACATTTTGTA CTTCGTCAAT AAATTTGTTT CGAAAAGTAA CTATGGTTGC 
TACACATCAA TTTCCGGAAC AAGAAGAACA CAGCCAATTT CTATATCCAA TGTGATTGAT 
ATTGAAGAAA ACATTTGGTC TCCAATATAT GGTCTTAAAG GGTTCCTAGA TGCAACTGTT 
GAAGCTAATG TTGAAAACAA TAAAAAACAC ATCGTTCCTT TAGAAGTGAA AACTGGAAAA 
TCCAGAAGCG TTTCATACGA GGTTCAAGGC CTTATTTATA CACTTCTGTT AAATGATCGC 
TATGAGATAC CGATAGAGTT TTTTCTTCTT TATTTTACGA GGGATAAAAA TATGACAAAG 
TTCCCTTCTG TTCTGCACTC CATTAAACAT ATTCTCATGT CCCGAAATAG AATGAGTATG 
AATTTTAAGC ATCAACTCCA AGAGGTTTTC GGACAGGCTC AATCAAGGTT TGAATTACCA 
CCCTTACTGC GCGATTCATC TTGTGATTCA TGTTTCATCA AAGAATCATG CATGGTGTTG 
AATAAGCTAC TAGAGGACGG GACACCAGAA GAAAGTGGCC TGGTAGAAGG TGAGTTTGAA 
ATTTTGACAA ACCATTTGTC GCAAAATCTC GCCAACTACA AAGAGTTTTT TACAAAATAT 
AATGATCTAA TAACTAAGGA GGAGTCAAGT ATAACATGTG TAAATAAAGA ACTTTTCTTA 
TTGGATGGTT CCACAAGAGA ATCCAGAAGT GGAAGGTGCC TTTCTGGCTT GGTTGTGTCA 
GAGGTGGTTG AACATGAAAA AACAGAGGGA GCGTATATAT ATTGCTTCTC AAGACGTAGA 
AATGATAATA ACTCGCAGTC GATGTTATCA TCTCAAATTG CTGCTAATGA CTTTGTTATT 
ATTAGTGATG AGGAGGGTCA TTTTTGCTTG TGCCAAGGTC GTGTACAATT TATAAATCCC 
GCTAAAATTG GTATCTCCGT AAAACGAAAG TTATTAAACA ATAGGCTTCT AGATAAGGAA 
AAAGGAGTCA CTACCATCCA AAGTGTGGTC GAATCAGAGC TAGAACAATC CAGTCTCATA 
GCTACCCAAA ATTTGGTCAC ATATAGAATC GACAAAAATG ATATCCAACA GAGTTTGTCT 
TTAGCGCGAT TTAATCTTTT AAGCTTGTTT TTACCAGCTG TTTCACCTGG CGTGGATATT 
GTTGACGAAA GGAGTAAATT ATGTCGGAAG ACAAAGAGAT CTGATGGAGG TAATGAAATA 
CTGCGATCAC TTTTAGTTGA TAATAGAGCA CCTAAGTTTA GAGATGCAAA TGATGATCCC 
GTCATTCCGT ATAAGCTTTC AAAGGATACA ACATTGAACT TGAATCAAAA AGAAGCAATA 
GACAAAGTAA TGAGGGCCGA AGATTATGCA TTGATATTGG GAATGCCAGG GACTGGTAAG 
ACTACTGTTA TCGCAGAAAT TATTAAAATT CTCGTTTCCG AGGGGAAACG CGTGCTTTTG 
ACATCATACA CACATTCAGC AGTGGATAAT ATTTTAATAA AATTGAGGAA CACCAACATT 
AGCATAATGA GATTGGGAAT GAAACATAAG GTCCATCCTG ACACGCAAAA ATATGTCCCG 
AATTACGCGT CAGTGAAAAG TTATAACGAT TATTTGTCCA AAATTAACAG CACCTCTGTT 
GTTGCTACCA CCTGCTTAGG AATTAACGAT ATACTATTTA CACTAAATGA AAAGGATTTT 
GATTATGTGA TTTTGGACGA GGCAAGTCAA ATTTCAATGC CTGTCGCTTT GGGACCTCTG 
CGGTATGGAA ATAGGTTTAT TATGGTAGGA GATCATTACC AGCTACCGCC ATTAGTTAAA 
AATGACGCAG CTCGATTAGG TGGGTTAGAA GAGTCACTGT TCAAGACTTT CTGTGAAAAG 
CATCCGGAAA GCGTTGCAGA ACTGACTTTA CAGTACCGTA TGTGCGGCGA TATTGTAACT 
TTGTCTAATT TCCTAATTTA TGATAATAAA TTAAAATGCG GGAATAACGA AGTTTTTGCT 
CAATCTTTAG AGTTACCAAT GCCTGAGGCC TTGTCGCGCT ATAGGAATGA ATCGGCTAAC 
TCTAAGCAGT GGCTTGAGGA TATACTAGAG CCTACAAGAA AAGTGGTATT TTTAAATTAC 
GATAACTGCC CGGATATTAT CGAACAAAGC GAAAAGGATA ACATTACGAA TCACGGGGAG 
GCAGAATTGA CTCTACAATG TGTGGAAGGA ATGTTATTGA GCGGCGTACC ATGCGAAGAT 
ATTGGTGTAA TGACGTTATA CAGAGCACAG TTAAGATTAC TGAAGAAGAT ATTCAACAAA 
AATGTGTATG ACGGGCTAGA GATCTTGACT GCTGATCAGT TTCAAGGTCG TGACAAAAAG 
TGCATTATTA TTTCCATGGT TAGAAGAAAT TCTCAATTAA ATGGAGGAGC GCTGCTGAAA 
GAGCTAAGAA GAGTCAATGT TGCCATGACA AGGGCCAAAT CCAAGCTGAT CATTATAGGT 
TCAAAAAGTA CAATCGGCTC TGTTCCTGAA ATAAAATCGT TCGTGAACCT GTTAGAAGAG 
CGTAATTGGG TCTATACAAT GTGCAAGGAT GCACTCTACA AATACAAATT TCCCGACAGA 
TCAAACGCTA TTGATGAGGC ACGCAAAGGT TGTGGAAAAA GAACAGGTGC CAAACCCATC 
ACATCAAAGT CTAAATTCGT AAGCGATAAA CCTATCATAA AGGAAATTCT ACAAGAGTAT 
GAAAGTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Life with 6000 genes.
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)
Goffeau A,Barrell BG,Bussey H,Davis RW,Dujon B,Feldmann H,Galibert F,Hoheisel JD,Jacq C,Johnston M,Louis EJ,Mewes HW,Murakami Y,Philippsen P,Tettelin H,Oliver SG

Complete nucleotide sequence of Saccharomyces cerevisiae chromosome VIII.
Science (New York, N.Y.)265(5181)2077-82(1994 Sep)
Johnston M,Andrews S,Brinkman R,Cooper J,Ding H,Dover J,Du Z,Favello A,Fulton L,Gattung S

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DNA2 cDNA ORF clone, Saccharomyces cerevisiae S288C

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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