NDT80 cDNA ORF clone, Saccharomyces cerevisiae S288C

Gene
Clones
gRNAs

Summary

Gene Symbol	NDT80
Entrez Gene ID	856524
Full Name	transcription factor NDT80
Gene Type	protein-coding
Organism	Saccharomyces cerevisiae S288C

mRNA and Protein(s)

mRNA	Protein	Name
NM_001179254.1	NP_011992.1	transcription factor NDT80

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Life with 6000 genes.
Goffeau A, Barrell BG, Bussey H, Davis RW, Dujon B, Feldmann H, Galibert F, Hoheisel JD, Jacq C, Johnston M, Louis EJ, Mewes HW, Murakami Y, Philippsen P, Tettelin H, Oliver SG
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)

Complete nucleotide sequence of Saccharomyces cerevisiae chromosome VIII.
Johnston M, Andrews S, Brinkman R, Cooper J, Ding H, Dover J, Du Z, Favello A, Fulton L, Gattung S
Science (New York, N.Y.)265(5181)2077-82(1994 Sep)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

ndt80 transcription of CDC5 results in degradation of Red1, a meiosis-specific protein required for Mek1 activation, thereby abolishing Mek1 activity completely. Elimination of Mek1 kinase activity allows Rad51-mediated repair of any remaining DSBs. In this way, cells do not enter Meiosis I until recombination is complete and all DSBs are repair
Title: Coordination of Double Strand Break Repair and Meiotic Progression in Yeast by a Mek1-Ndt80 Negative Feedback Loop.

The findings suggest that irreversibility of meiotic commitment is a consequence of the NDT80 transcriptional positive feedback loop, which provides the high-level of Ndt80 required for the developmental switch of meiotic commitment.
Title: Positive feedback of NDT80 expression ensures irreversible meiotic commitment in budding yeast.

Our results reveal a role for Cdc5 in meiotic checkpoint control by regulating Ndt80 function
Title: The budding yeast polo-like kinase Cdc5 regulates the Ndt80 branch of the meiotic recombination checkpoint pathway.

nuclear localization of Ndt80 is regulated by the pachytene checkpoint through a cytoplasmic anchor mechanism
Title: Nuclear localization of the meiosis-specific transcription factor Ndt80 is regulated by the pachytene checkpoint.

transient induction of a transcription factor (NDT80) essential for later stages of gametogenesis extends the replicative life span of aged cells
Title: Gametogenesis eliminates age-induced cellular damage and resets life span in yeast.

The following NDT80 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the NDT80 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OSi05322	NM_001179254.1 Latest version!	Saccharomyces cerevisiae S288C transcription factor NDT80 (NDT80), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

NDT80 ( NM_001179254.1 ) cDNA ORF clone, Saccharomyces cerevisiae S288C -> NP_011992.1 Saccharomyces cerevisiae S288C transcription factor NDT80 (NDT80), partial mRNA.

Price & Availability↑

CloneID	OSi05322
Clone ID Related Accession (Same CDS sequence)	NM_001179254.1
Accession Version	NM_001179254.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1884bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-31
Organism	Saccharomyces cerevisiae S288C
Product	transcription factor NDT80
Comment	Comment: REVIEWED REFSEQ: This record has been curated by SGD. This record is derived from an annotated genomic sequence (NC_001140). ##Genome-Annotation-Data-START## Annotation Provider :: SGD Annotation Status :: Full Annotation Annotation Veresion :: R64-2-1 URL :: http://www.yeastgenome.org/ ##Genome-Annotation-Data-END## COMPLETENESS: incomplete on both ends.

ATGAATGAAA TGGAAAACAC AGATCCAGTA TTACAGGATG ATCTCGTATC CAAATATGAG 
AGGGAATTAA GCACAGAACA GGAAGAGGAT ACGCCTGTTA TCCTTACGCA GTTGAATGAA 
GATGGCACCA CTTCGAATTA TTTCGACAAA AGGAAACTAA AAATTGCCCC TAGATCAACA 
CTACAGTTCA AAGTGGGACC TCCATTTGAA TTAGTGAGAG ATTATTGTCC AGTCGTTGAG 
TCTCATACCG GAAGAACACT AGATTTACGA ATTATTCCAA GAATTGACAG AGGGTTCGAC 
CATATTGATG AAGAGTGGGT AGGTTATAAA AGAAATTATT TTACCTTAGT ATCAACGTTT 
GAAACGGCAA ATTGTGATTT GGATACTTTT TTAAAGAGCA GTTTTGATCT TCTCGTTGAA 
GACTCTTCAG TAGAAAGCAG ATTAAGAGTG CAATATTTCG CTATCAAAAT AAAAGCTAAG 
AATGACGACG ACGACACGGA AATCAATCTC GTCCAGCATA CAGCGAAACG CGACAAAGGT 
CCTCAATTTT GTCCTTCAGT ATGTCCGTTG GTGCCTTCCC CTTTGCCAAA ACATCAAATC 
ATAAGAGAAG CTTCAAATGT TCGAAATATC ACTAAAATGA AAAAATACGA TTCCACTTTT 
TATTTGCACA GAGACCACGT TAATTATGAA GAATATGGAG TGGACTCTTT ATTGTTTTCC 
TATCCAGAAG ATTCTATTCA GAAAGTTGCC CGTTATGAAA GAGTTCAATT TGCTTCATCA 
ATTAGCGTGA AGAAACCATC CCAACAAAAT AAACACTTTA GCTTGCATGT AATTTTAGGT 
GCAGTGGTAG ATCCAGATAC CTTTCATGGG GAGAATCCCG GAATTCCTTA TGATGAACTG 
GCTTTAAAAA ATGGATCAAA AGGGATGTTT GTGTATTTGC AAGAAATGAA AACGCCTCCT 
CTTATTATTA GAGGAAGATC ACCTTCTAAC TATGCGTCAT CTCAGCGAAT AACTGTGAGA 
ACACCGTCGA GTGTCAATTC CTCACAAAAC AGCACAAAAA GAAAAATGCC ATCAATGGCG 
CAGCCGTTAA ATGAAAGTTG CTTAAATGCA AGACCTTCGA AAAGGCGATC CAAAGTGGCG 
CTAGGTGCAC CGAACTCTGG GGCCTCCATC TCGCCTATCA AATCTCGTCA ATCCACACCA 
ATGGAAGCTT CGAAGGAAAA TGAGGATCCG TTCTTCAGGC CAAATAAAAG GGTGGAGACT 
CTTGAACATA TCCAGAACAA ACTGGGTGCT TTGAAAAATC AATGTCCAGA TTCCTCTCTG 
AAATATCCGA GTTCATCTTC AAGAGGTATG GAAGGGTGTT TAGAAAAGGA GGATTTAGTT 
TACTCAAGTA GTTTTTCTGT TAATATGAAG CAAATCGAAC TGAAACCGGC ACGCTCTTTT 
GAACATGAGA ATATTTTCAA AGTAGGCTCA TTAGCATTCA AAAAAATCAA TGAATTACCT 
CATGAAAATT ATGATATAAC AATAGAGAAG AAATCAATGG AACAGAATTA TCTAAGACCA 
GAGATAGGCT CACGTTCTGA ATGCAAAACA AGCTATGGTA ACGAACTCTC CCTTTCAAAT 
ATTTCTTTCT CTATACTACC TAACTCAGCA GAAAATTTTC ACTTGGAAAC TGCACTTTTC 
CCAGCTACGG AAGAGGATGT GCCAAGGACA TTTTCAAGAA TATTAGAAAC AGGTTCGTTT 
CAAAATTATT ATCAAAAAAT GGATGCAGAA AATGCAGATA GGGTATATTC GAAAGGGGTC 
AAGTTGATTG CAAGCGGTAC TCTACCATCT GGTATATTTA ATAGGGAAGA ATTGTTTGAG 
GAAGATAGTT TCTATAAGTA TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_011992.1
CDS	1..1884
Translation	MNEMENTDPVLQDDLVSKYERELSTEQEEDTPVILTQLNEDGTTSNYFDKRKLKIAPRSTLQFKVGPPFELVRDYCPVVESHTGRTLDLRIIPRIDRGFDHIDEEWVGYKRNYFTLVSTFETANCDLDTFLKSSFDLLVEDSSVESRLRVQYFAIKIKAKNDDDDTEINLVQHTAKRDKGPQFCPSVCPLVPSPLPKHQIIREASNVRNITKMKKYDSTFYLHRDHVNYEEYGVDSLLFSYPEDSIQKVARYERVQFASSISVKKPSQQNKHFSLHVILGAVVDPDTFHGENPGIPYDELALKNGSKGMFVYLQEMKTPPLIIRGRSPSNYASSQRITVRTPSSVNSSQNSTKRKMPSMAQPLNESCLNARPSKRRSKVALGAPNSGASISPIKSRQSTPMEASKENEDPFFRPNKRVETLEHIQNKLGALKNQCPDSSLKYPSSSSRGMEGCLEKEDLVYSSSFSVNMKQIELKPARSFEHENIFKVGSLAFKKINELPHENYDITIEKKSMEQNYLRPEIGSRSECKTSYGNELSLSNISFSILPNSAENFHLETALFPATEEDVPRTFSRILETGSFQNYYQKMDAENADRVYSKGVKLIASGTLPSGIFNREELFEEDSFYKY

Target ORF information:

RefSeq Version	NM_001179254.1
Organism	Saccharomyces cerevisiae S288C
Definition	Saccharomyces cerevisiae S288C transcription factor NDT80 (NDT80), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001179254.1

ORF Insert Sequence:

ATGAATGAAA TGGAAAACAC AGATCCAGTA TTACAGGATG ATCTCGTATC CAAATATGAG 
AGGGAATTAA GCACAGAACA GGAAGAGGAT ACGCCTGTTA TCCTTACGCA GTTGAATGAA 
GATGGCACCA CTTCGAATTA TTTCGACAAA AGGAAACTAA AAATTGCCCC TAGATCAACA 
CTACAGTTCA AAGTGGGACC TCCATTTGAA TTAGTGAGAG ATTATTGTCC AGTCGTTGAG 
TCTCATACCG GAAGAACACT AGATTTACGA ATTATTCCAA GAATTGACAG AGGGTTCGAC 
CATATTGATG AAGAGTGGGT AGGTTATAAA AGAAATTATT TTACCTTAGT ATCAACGTTT 
GAAACGGCAA ATTGTGATTT GGATACTTTT TTAAAGAGCA GTTTTGATCT TCTCGTTGAA 
GACTCTTCAG TAGAAAGCAG ATTAAGAGTG CAATATTTCG CTATCAAAAT AAAAGCTAAG 
AATGACGACG ACGACACGGA AATCAATCTC GTCCAGCATA CAGCGAAACG CGACAAAGGT 
CCTCAATTTT GTCCTTCAGT ATGTCCGTTG GTGCCTTCCC CTTTGCCAAA ACATCAAATC 
ATAAGAGAAG CTTCAAATGT TCGAAATATC ACTAAAATGA AAAAATACGA TTCCACTTTT 
TATTTGCACA GAGACCACGT TAATTATGAA GAATATGGAG TGGACTCTTT ATTGTTTTCC 
TATCCAGAAG ATTCTATTCA GAAAGTTGCC CGTTATGAAA GAGTTCAATT TGCTTCATCA 
ATTAGCGTGA AGAAACCATC CCAACAAAAT AAACACTTTA GCTTGCATGT AATTTTAGGT 
GCAGTGGTAG ATCCAGATAC CTTTCATGGG GAGAATCCCG GAATTCCTTA TGATGAACTG 
GCTTTAAAAA ATGGATCAAA AGGGATGTTT GTGTATTTGC AAGAAATGAA AACGCCTCCT 
CTTATTATTA GAGGAAGATC ACCTTCTAAC TATGCGTCAT CTCAGCGAAT AACTGTGAGA 
ACACCGTCGA GTGTCAATTC CTCACAAAAC AGCACAAAAA GAAAAATGCC ATCAATGGCG 
CAGCCGTTAA ATGAAAGTTG CTTAAATGCA AGACCTTCGA AAAGGCGATC CAAAGTGGCG 
CTAGGTGCAC CGAACTCTGG GGCCTCCATC TCGCCTATCA AATCTCGTCA ATCCACACCA 
ATGGAAGCTT CGAAGGAAAA TGAGGATCCG TTCTTCAGGC CAAATAAAAG GGTGGAGACT 
CTTGAACATA TCCAGAACAA ACTGGGTGCT TTGAAAAATC AATGTCCAGA TTCCTCTCTG 
AAATATCCGA GTTCATCTTC AAGAGGTATG GAAGGGTGTT TAGAAAAGGA GGATTTAGTT 
TACTCAAGTA GTTTTTCTGT TAATATGAAG CAAATCGAAC TGAAACCGGC ACGCTCTTTT 
GAACATGAGA ATATTTTCAA AGTAGGCTCA TTAGCATTCA AAAAAATCAA TGAATTACCT 
CATGAAAATT ATGATATAAC AATAGAGAAG AAATCAATGG AACAGAATTA TCTAAGACCA 
GAGATAGGCT CACGTTCTGA ATGCAAAACA AGCTATGGTA ACGAACTCTC CCTTTCAAAT 
ATTTCTTTCT CTATACTACC TAACTCAGCA GAAAATTTTC ACTTGGAAAC TGCACTTTTC 
CCAGCTACGG AAGAGGATGT GCCAAGGACA TTTTCAAGAA TATTAGAAAC AGGTTCGTTT 
CAAAATTATT ATCAAAAAAT GGATGCAGAA AATGCAGATA GGGTATATTC GAAAGGGGTC 
AAGTTGATTG CAAGCGGTAC TCTACCATCT GGTATATTTA ATAGGGAAGA ATTGTTTGAG 
GAAGATAGTT TCTATAAGTA TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Life with 6000 genes.
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)
Goffeau A,Barrell BG,Bussey H,Davis RW,Dujon B,Feldmann H,Galibert F,Hoheisel JD,Jacq C,Johnston M,Louis EJ,Mewes HW,Murakami Y,Philippsen P,Tettelin H,Oliver SG

Complete nucleotide sequence of Saccharomyces cerevisiae chromosome VIII.
Science (New York, N.Y.)265(5181)2077-82(1994 Sep)
Johnston M,Andrews S,Brinkman R,Cooper J,Ding H,Dover J,Du Z,Favello A,Fulton L,Gattung S

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NDT80 cDNA ORF clone, Saccharomyces cerevisiae S288C

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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