DMA1 cDNA ORF clone, Saccharomyces cerevisiae S288C

Gene
Clones
gRNAs

Summary

Gene Symbol	DMA1
Entrez Gene ID	856515
Full Name	ubiquitin-conjugating protein DMA1
Synonyms	CHF1
Gene Type	protein-coding
Organism	Saccharomyces cerevisiae S288C

mRNA and Protein(s)

mRNA	Protein	Name
NM_001179245.1	NP_011983.1	ubiquitin-conjugating protein DMA1

Pathways from BioSystems

Homologies

Saccharomyces cerevisiae (baker's yeast)	DMA2	NP_014283.3
Saccharomyces cerevisiae (baker's yeast)	DMA1	NP_011983.1
Kluyveromyces lactis	KLLA0F23892g	XP_456144.1
Eremothecium gossypii	AGOS_ACR267C	NP_983669.1

Gene Ontology
Bibliography

Related articles in PubMed

Life with 6000 genes.
Goffeau A, Barrell BG, Bussey H, Davis RW, Dujon B, Feldmann H, Galibert F, Hoheisel JD, Jacq C, Johnston M, Louis EJ, Mewes HW, Murakami Y, Philippsen P, Tettelin H, Oliver SG
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)

Complete nucleotide sequence of Saccharomyces cerevisiae chromosome VIII.
Johnston M, Andrews S, Brinkman R, Cooper J, Ding H, Dover J, Du Z, Favello A, Fulton L, Gattung S
Science (New York, N.Y.)265(5181)2077-82(1994 Sep)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Dma1/2 mediate rapid Sld2 degradation by recognizing a complex phospho-degron
Title: Robust Replication Control Is Generated by Temporal Gaps between Licensing and Firing Phases and Depends on Degradation of Firing Factor Sld2.

Expression of a hyperactive variant of Bni1 (Bni1-V360D) rescues these defects and partially restores proper spindle positioning in the mutant, suggesting that the failure of dma1 dma2 mutant cells to position the spindle is partly due to faulty formin activity.
Title: Control of Formin Distribution and Actin Cable Assembly by the E3 Ubiquitin Ligases Dma1 and Dma2.

Defective in mitotic arrest 1 (Dma1) ubiquitin ligase controls G1 cyclin degradation
Title: Defective in mitotic arrest 1 (Dma1) ubiquitin ligase controls G1 cyclin degradation.

Dma1 and Dma2 control septin dynamics and the SPOC by promoting the efficient recruitment of Elm1 to the bud neck
Title: Budding yeast dma proteins control septin dynamics and the spindle position checkpoint by promoting the recruitment of the Elm1 kinase to the bud neck.

Chfr homologs have dual ubiquitin conjugating enzyme specificity; accounting for different functions in the cell cycle.
Title: Yeast Chfr homologs retard cell cycle at G1 and G2/M via Ubc4 and Ubc13/Mms2-dependent ubiquitination.

The following DMA1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the DMA1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OSi05313	NM_001179245.1 Latest version!	Saccharomyces cerevisiae S288C ubiquitin-conjugating protein DMA1 (DMA1), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

DMA1 ( NM_001179245.1 ) cDNA ORF clone, Saccharomyces cerevisiae S288C -> NP_011983.1 Saccharomyces cerevisiae S288C ubiquitin-conjugating protein DMA1 (DMA1), partial mRNA.

Price & Availability↑

CloneID	OSi05313
Clone ID Related Accession (Same CDS sequence)	NM_001179245.1
Accession Version	NM_001179245.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1251bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-31
Organism	Saccharomyces cerevisiae S288C
Product	ubiquitin-conjugating protein DMA1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by SGD. This record is derived from an annotated genomic sequence (NC_001140). ##Genome-Annotation-Data-START## Annotation Provider :: SGD Annotation Status :: Full Annotation Annotation Veresion :: R64-2-1 URL :: http://www.yeastgenome.org/ ##Genome-Annotation-Data-END## COMPLETENESS: incomplete on both ends.

ATGTCTACTA ATACTGTTCC CTCATCTCCC CCAAACCAGA CGCCACCTGC TGCTTCTGGC 
ATCGCTACCT CTCACGATCA CACCAAGTTC AACAATCCCA TCAGACTACC GATATCCATC 
TCTCTCACCA TTAACGATAC GCCTAACAAT AACAGCAATA ATAATAGCGT TAGCAATGGT 
TTAGGTATTT TGCCCTCGCG TACGGCTACT TCTTTAGTCG TTGCAAATAA CGGTAGCGCA 
AACGGCAATG TTGGTGCTAC TGCTGCTGCT GCTGCCACTG TGGAGACGAA TACAGCCCCC 
GCGGTGAACA CTACAAAGAG TATCCGCCAT TTCATATATC CTCCAAACCA AGTCAATCAG 
ACGGAGTTTT CACTTGATAT ACATCTCCCA CCAAATACCT CGCTACCGGA ACGGATAGAC 
CAATCTACTT TGAAACGCAG AATGGACAAG CACGGGCTTT TCAGCATTAG GCTAACCCCG 
TTTATAGATA CTTCATCCAC GTCTGTGGCT AACCAGGGTC TTTTCTTTGA CCCCATTATC 
AGGACTGCGG GCGCAGGCTC GCAAATTATC ATCGGAAGAT ACACCGAAAG AGTAAGAGAG 
GCAATTTCCA AAATTCCAGA TCAGTATCAT CCCGTAGTGT TCAAATCAAA AGTGATATCC 
AGGACCCATG GGTGTTTCAA AGTTGATGAC CAAGGAAACT GGTTTTTGAA AGATGTCAAG 
TCTTCCAGTG GGACCTTTTT GAACCACCAG CGTCTATCTT CTGCGTCTAC CACTTCTAAG 
GACTACCTTT TACATGACGG AGATATCATA CAGCTGGGTA TGGACTTTCG CGGCGGGACG 
GAAGAGATAT ATCGTTGTGT TAAGATGAAA ATAGAATTGA ACAAGTCTTG GAAATTAAAG 
GCCAATGCAT TTAATAAAGA AGCCTTGAGC CGAATAAAAA ATCTACAGAA ACTAACTACT 
GGTTTAGAAC AAGAAGACTG TTCCATTTGT CTAAACAAAA TAAAACCTTG CCAAGCCATT 
TTTATATCAC CATGTGCCCA TAGTTGGCAT TTTCATTGTG TAAGAAGATT GGTTATTATG 
AATTATCCTC AATTCATGTG TCCGAACTGT AGGACAAACT GTGATTTGGA GACTACCTTG 
GAATCCGAAT CTGAATCCGA ATTCGAAAAC GAGGACGAAG ATGAGCCAGA CATTGAAATG 
GATATAGACA TGGAAATAAA TAACAATTTA GGTGTTCGCC TAGTAGATTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_011983.1
CDS	1..1251
Translation	MSTNTVPSSPPNQTPPAASGIATSHDHTKFNNPIRLPISISLTINDTPNNNSNNNSVSNGLGILPSRTATSLVVANNGSANGNVGATAAAAATVETNTAPAVNTTKSIRHFIYPPNQVNQTEFSLDIHLPPNTSLPERIDQSTLKRRMDKHGLFSIRLTPFIDTSSTSVANQGLFFDPIIRTAGAGSQIIIGRYTERVREAISKIPDQYHPVVFKSKVISRTHGCFKVDDQGNWFLKDVKSSSGTFLNHQRLSSASTTSKDYLLHDGDIIQLGMDFRGGTEEIYRCVKMKIELNKSWKLKANAFNKEALSRIKNLQKLTTGLEQEDCSICLNKIKPCQAIFISPCAHSWHFHCVRRLVIMNYPQFMCPNCRTNCDLETTLESESESEFENEDEDEPDIEMDIDMEINNNLGVRLVD

Target ORF information:

RefSeq Version	NM_001179245.1
Organism	Saccharomyces cerevisiae S288C
Definition	Saccharomyces cerevisiae S288C ubiquitin-conjugating protein DMA1 (DMA1), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001179245.1

ORF Insert Sequence:

ATGTCTACTA ATACTGTTCC CTCATCTCCC CCAAACCAGA CGCCACCTGC TGCTTCTGGC 
ATCGCTACCT CTCACGATCA CACCAAGTTC AACAATCCCA TCAGACTACC GATATCCATC 
TCTCTCACCA TTAACGATAC GCCTAACAAT AACAGCAATA ATAATAGCGT TAGCAATGGT 
TTAGGTATTT TGCCCTCGCG TACGGCTACT TCTTTAGTCG TTGCAAATAA CGGTAGCGCA 
AACGGCAATG TTGGTGCTAC TGCTGCTGCT GCTGCCACTG TGGAGACGAA TACAGCCCCC 
GCGGTGAACA CTACAAAGAG TATCCGCCAT TTCATATATC CTCCAAACCA AGTCAATCAG 
ACGGAGTTTT CACTTGATAT ACATCTCCCA CCAAATACCT CGCTACCGGA ACGGATAGAC 
CAATCTACTT TGAAACGCAG AATGGACAAG CACGGGCTTT TCAGCATTAG GCTAACCCCG 
TTTATAGATA CTTCATCCAC GTCTGTGGCT AACCAGGGTC TTTTCTTTGA CCCCATTATC 
AGGACTGCGG GCGCAGGCTC GCAAATTATC ATCGGAAGAT ACACCGAAAG AGTAAGAGAG 
GCAATTTCCA AAATTCCAGA TCAGTATCAT CCCGTAGTGT TCAAATCAAA AGTGATATCC 
AGGACCCATG GGTGTTTCAA AGTTGATGAC CAAGGAAACT GGTTTTTGAA AGATGTCAAG 
TCTTCCAGTG GGACCTTTTT GAACCACCAG CGTCTATCTT CTGCGTCTAC CACTTCTAAG 
GACTACCTTT TACATGACGG AGATATCATA CAGCTGGGTA TGGACTTTCG CGGCGGGACG 
GAAGAGATAT ATCGTTGTGT TAAGATGAAA ATAGAATTGA ACAAGTCTTG GAAATTAAAG 
GCCAATGCAT TTAATAAAGA AGCCTTGAGC CGAATAAAAA ATCTACAGAA ACTAACTACT 
GGTTTAGAAC AAGAAGACTG TTCCATTTGT CTAAACAAAA TAAAACCTTG CCAAGCCATT 
TTTATATCAC CATGTGCCCA TAGTTGGCAT TTTCATTGTG TAAGAAGATT GGTTATTATG 
AATTATCCTC AATTCATGTG TCCGAACTGT AGGACAAACT GTGATTTGGA GACTACCTTG 
GAATCCGAAT CTGAATCCGA ATTCGAAAAC GAGGACGAAG ATGAGCCAGA CATTGAAATG 
GATATAGACA TGGAAATAAA TAACAATTTA GGTGTTCGCC TAGTAGATTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Life with 6000 genes.
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)
Goffeau A,Barrell BG,Bussey H,Davis RW,Dujon B,Feldmann H,Galibert F,Hoheisel JD,Jacq C,Johnston M,Louis EJ,Mewes HW,Murakami Y,Philippsen P,Tettelin H,Oliver SG

Complete nucleotide sequence of Saccharomyces cerevisiae chromosome VIII.
Science (New York, N.Y.)265(5181)2077-82(1994 Sep)
Johnston M,Andrews S,Brinkman R,Cooper J,Ding H,Dover J,Du Z,Favello A,Fulton L,Gattung S

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DMA1 cDNA ORF clone, Saccharomyces cerevisiae S288C

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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