SPO11 cDNA ORF clone, Saccharomyces cerevisiae S288C

The following SPO11 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the SPO11 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OSi05173 NM_001179102.1
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Saccharomyces cerevisiae S288C DNA topoisomerase (ATP-hydrolyzing) (SPO11), partial mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $258.30
$369.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OSi05173
    Clone ID Related Accession (Same CDS sequence) NM_001179102.1
    Accession Version NM_001179102.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1197bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-10-31
    Organism Saccharomyces cerevisiae S288C
    Product DNA topoisomerase (ATP-hydrolyzing)
    Comment Comment: REVIEWED REFSEQ: This record has been curated by SGD. This record is derived from an annotated genomic sequence (NC_001140). ##Genome-Annotation-Data-START## Annotation Provider :: SGD Annotation Status :: Full Annotation Annotation Veresion :: R64-2-1 URL :: http://www.yeastgenome.org/ ##Genome-Annotation-Data-END## COMPLETENESS: incomplete on both ends.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    ATGGCTTTGG AGGGATTGCG GAAAAAATAT AAAACAAGGC AGGAATTGGT CAAAGCACTC 
    ACTCCTAAAA GACGGTCCAT TCACTTGAAC TCCAATGGTC ACTCCAACGG AACTCCCTGT
    TCAAACGCAG ATGTTTTGGC TCATATTAAG CATTTCCTGT CATTGGCGGC TAATTCATTA
    GAGCAACATC AACAGCCTAT TTCAATCGTC TTTCAAAACA AAAAAAAAAA AGGCGATACA
    AGCAGTCCTG ACATTCACAC AACATTGGAC TTCCCTTTGA ATGGCCCGCA TCTATGCACT
    CATCAGTTCA AGTTGAAAAG ATGCGCAATC CTTTTAAACT TATTGAAAGT CGTTATGGAA
    AAATTACCGC TAGGTAAAAA CACTACAGTG AGAGATATCT TCTACTCCAA CGTGGAATTG
    TTTCAAAGAC AAGCAAACGT AGTCCAGTGG CTGGACGTTA TACGCTTTAA TTTCAAGCTC
    TCTCCAAGAA AATCCTTAAA CATTATACCA GCTCAAAAGG GTTTAGTTTA TTCGCCTTTC
    CCCATTGATA TTTATGACAA TATTCTGACA TGTGAAAATG AACCAAAGAT GCAAAAGCAA
    ACAATTTTCC CTGGTAAGCC CTGTCTAATT CCATTTTTCC AAGATGATGC GGTCATCAAG
    TTAGGGACAA CAAGTATGTG TAATATTGTA ATAGTGGAAA AAGAAGCTGT CTTCACCAAA
    TTAGTAAATA ATTATCACAA GTTGAGTACA AATACCATGC TCATTACAGG TAAGGGATTT
    CCAGATTTCT TGACAAGGTT ATTCCTAAAA AAACTAGAAC AATATTGCTC CAAATTGATA
    TCGGACTGTT CTATATTTAC CGATGCGGAC CCCTATGGGA TTAGCATAGC CCTAAATTAT
    ACTCACTCGA ATGAACGCAA CGCTTATATT TGCACGATGG CAAACTATAA AGGAATTCGT
    ATTACGCAAG TTTTGGCACA AAATAATGAA GTGCATAACA AATCCATTCA ATTATTGAGT
    TTGAATCAGC GCGACTACTC CTTAGCCAAG AATTTGATAG CATCTCTGAC TGCCAACAGC
    TGGGATATTG CAACTTCACC ATTAAAGAAC GTCATCATAG AATGTCAGCG GGAAATTTTT
    TTCCAAAAGA AAGCTGAAAT GAACGAGATT GATGCCAGAA TTTTTGAATA CAAATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_011841.1
    CDS1..1197
    Translation

    Target ORF information:

    RefSeq Version NM_001179102.1
    Organism Saccharomyces cerevisiae S288C
    Definition Saccharomyces cerevisiae S288C DNA topoisomerase (ATP-hydrolyzing) (SPO11), partial mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001179102.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    ATGGCTTTGG AGGGATTGCG GAAAAAATAT AAAACAAGGC AGGAATTGGT CAAAGCACTC 
    ACTCCTAAAA GACGGTCCAT TCACTTGAAC TCCAATGGTC ACTCCAACGG AACTCCCTGT
    TCAAACGCAG ATGTTTTGGC TCATATTAAG CATTTCCTGT CATTGGCGGC TAATTCATTA
    GAGCAACATC AACAGCCTAT TTCAATCGTC TTTCAAAACA AAAAAAAAAA AGGCGATACA
    AGCAGTCCTG ACATTCACAC AACATTGGAC TTCCCTTTGA ATGGCCCGCA TCTATGCACT
    CATCAGTTCA AGTTGAAAAG ATGCGCAATC CTTTTAAACT TATTGAAAGT CGTTATGGAA
    AAATTACCGC TAGGTAAAAA CACTACAGTG AGAGATATCT TCTACTCCAA CGTGGAATTG
    TTTCAAAGAC AAGCAAACGT AGTCCAGTGG CTGGACGTTA TACGCTTTAA TTTCAAGCTC
    TCTCCAAGAA AATCCTTAAA CATTATACCA GCTCAAAAGG GTTTAGTTTA TTCGCCTTTC
    CCCATTGATA TTTATGACAA TATTCTGACA TGTGAAAATG AACCAAAGAT GCAAAAGCAA
    ACAATTTTCC CTGGTAAGCC CTGTCTAATT CCATTTTTCC AAGATGATGC GGTCATCAAG
    TTAGGGACAA CAAGTATGTG TAATATTGTA ATAGTGGAAA AAGAAGCTGT CTTCACCAAA
    TTAGTAAATA ATTATCACAA GTTGAGTACA AATACCATGC TCATTACAGG TAAGGGATTT
    CCAGATTTCT TGACAAGGTT ATTCCTAAAA AAACTAGAAC AATATTGCTC CAAATTGATA
    TCGGACTGTT CTATATTTAC CGATGCGGAC CCCTATGGGA TTAGCATAGC CCTAAATTAT
    ACTCACTCGA ATGAACGCAA CGCTTATATT TGCACGATGG CAAACTATAA AGGAATTCGT
    ATTACGCAAG TTTTGGCACA AAATAATGAA GTGCATAACA AATCCATTCA ATTATTGAGT
    TTGAATCAGC GCGACTACTC CTTAGCCAAG AATTTGATAG CATCTCTGAC TGCCAACAGC
    TGGGATATTG CAACTTCACC ATTAAAGAAC GTCATCATAG AATGTCAGCG GGAAATTTTT
    TTCCAAAAGA AAGCTGAAAT GAACGAGATT GATGCCAGAA TTTTTGAATA CAAATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.