NAB3 cDNA ORF clone, Saccharomyces cerevisiae S288C

Gene
Clones
gRNAs

Summary

Gene Symbol	NAB3
Entrez Gene ID	855911
Full Name	Nab3p
Synonyms	HMD1
Gene Type	protein-coding
Organism	Saccharomyces cerevisiae S288C

mRNA and Protein(s)

mRNA	Protein	Name
NM_001184004.1	NP_015134.1	Nab3p

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

The nucleotide sequence of Saccharomyces cerevisiae chromosome XVI.
Bussey H, Storms RK, Ahmed A, Albermann K, Allen E, Ansorge W, Araujo R, Aparicio A, Barrell B, Badcock K, Benes V, Botstein D, Bowman S, Br?ckner M, Carpenter J, Cherry JM, Chung E, Churcher C, Coster F, Davis K, Davis RW, Dietrich FS, Delius H, DiPaolo T, Hani J
Nature387(6632 Suppl)103-5(1997 May)

Life with 6000 genes.
Goffeau A, Barrell BG, Bussey H, Davis RW, Dujon B, Feldmann H, Galibert F, Hoheisel JD, Jacq C, Johnston M, Louis EJ, Mewes HW, Murakami Y, Philippsen P, Tettelin H, Oliver SG
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Kinetic cross-linking and analysis of cDNAs uncovers a role for Nab3 in determining gene expression profiles during stress.
Title: Kinetic CRAC uncovers a role for Nab3 in determining gene expression profiles during stress.

Study analyzed the functional role of the prion-like low complexity domains (LCDs) of Nab3, which can assemble into a robust amyloid form. Results show that Nab3 LCD can be exchanged for the low complexity regions of other proteins as the prion protein Sup35, or the RNA-binding protein, Hrp1. Also, its C-terminus seems more complex than previously appreciated.
Title: The hnRNP-like Nab3 termination factor can employ heterologous prion-like domains in place of its own essential low complexity domain.

The data raise the intriguing possibility that Nab3 and Nrd1 can function independently to recruit Rrp6 to ncRNA targets, providing combinatorial flexibility in RNA processing.
Title: Nab3 facilitates the function of the TRAMP complex in RNA processing via recruitment of Rrp6 independent of Nrd1.

Rrp6 acts with the NNS complex(Nrd1, Nab3, and Sen1 ) globally to promote transcription termination in addition to 3' end RNA processing and/or degradation at specific targets.
Title: The exosome component Rrp6 is required for RNA polymerase II termination at specific targets of the Nrd1-Nab3 pathway.

Nrd1 and Nab3 function is tightly integrated with the nutrient response, and indicates a role for these proteins in the regulation of many mRNA coding genes.
Title: PAR-CLIP data indicate that Nrd1-Nab3-dependent transcription termination regulates expression of hundreds of protein coding genes in yeast.

The following NAB3 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the NAB3 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OSi04785	NM_001184004.1 Latest version!	Saccharomyces cerevisiae S288C Nab3p (NAB3), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$426.30 $609.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

NAB3 ( NM_001184004.1 ) cDNA ORF clone, Saccharomyces cerevisiae S288C -> NP_015134.1 Saccharomyces cerevisiae S288C Nab3p (NAB3), partial mRNA.

Price & Availability↑

CloneID	OSi04785
Clone ID Related Accession (Same CDS sequence)	NM_001184004.1
Accession Version	NM_001184004.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2409bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-31
Organism	Saccharomyces cerevisiae S288C
Product	Nab3p
Comment	Comment: REVIEWED REFSEQ: This record has been curated by SGD. This record is derived from an annotated genomic sequence (NC_001148). ##Genome-Annotation-Data-START## Annotation Provider :: SGD Annotation Status :: Full Annotation Annotation Version :: R64-2-1 URL :: http://www.yeastgenome.org/ ##Genome-Annotation-Data-END## COMPLETENESS: incomplete on both ends.

ATGTCAGATG AAAACCATAA CAGTGATGTT CAAGATATTC CTTCACCTGA ACTATCCGTC 
GATAGTAACT CTAACGAGAA TGAATTGATG AATAACTCAA GCGCAGACGA TGGAATCGAA 
TTTGACGCCC CAGAGGAAGA AAGAGAAGCC GAAAGGGAGG AGGAAAATGA AGAACAACAC 
GAACTGGAAG ATGTGAACGA TGAAGAGGAG GAAGATAAGG AGGAAAAAGG AGAGGAAAAC 
GGGGAAGTAA TAAACACAGA AGAAGAAGAA GAAGAAGAAC ATCAACAAAA AGGCGGAAAT 
GATGATGACG ATGATGATAA TGAAGAGGAA GAAGAGGAAG AAGAGGATGA TGACGATGAT 
GATGATGACG ACGATGATGA TGAAGAAGAA GAAGAAGAAG AAGAAGAAGA AGGCAACGAC 
AACAGTTCGG TAGGCTCAGA TAGTGCCGCT GAAGACGGTG AGGATGAGGA AGACAAAAAG 
GATAAAACCA AAGATAAAGA GGTCGAACTT CGCCGTGAAA CATTGGAAAA AGAACAAAAG 
GACGTAGATG AAGCTATAAA AAAAATAACT CGTGAAGAAA ATGATAATAC TCACTTTCCA 
ACTAATATGG AAAATGTTAA TTACGATCTT TTACAAAAGC AAGTCAAGTA CATTATGGAC 
AGTAACATGC TAAATTTGCC TCAGTTTCAA CATTTACCTC AAGAAGAAAA GATGTCTGCG 
ATTTTAGCAA TGTTAAATTC AAATTCTGAC ACAGCTCTTT CCGTACCTCC TCATGATAGT 
ACTATCTCAA CAACAGCTAG CGCCTCAGCC ACAAGCGGCG CAAGAAGCAA TGATCAAAGA 
AAACCTCCAT TGTCAGATGC CCAAAGACGT ATGAGATTTC CTAGGGCGGA TTTATCTAAG 
CCGATTACCG AAGAAGAACA CGACCGTTAT GCAGCCTATT TGCACGGTGA AAATAAAATC 
ACCGAGATGC ACAATATTCC TCCGAAGTCA AGATTATTCA TTGGTAATTT GCCGCTAAAG 
AACGTTTCTA AGGAGGATTT ATTTAGGATT TTCTCTCCAT ACGGTCATAT CATGCAAATC 
AATATCAAAA ATGCCTTTGG ATTCATTCAG TTTGACAACC CTCAAAGCGT TAGAGATGCA 
ATTGAATGCG AGTCTCAAGA AATGAACTTT GGCAAAAAGT TGATCCTGGA AGTTTCTAGC 
TCGAATGCTC GTCCTCAATT TGATCATGGT GATCACGGTA CAAACAGTAG TTCTACTTTT 
ATTTCTTCCG CAAAACGACC ATTTCAAACT GAATCTGGTG ACATGTACAA TGACGACAAT 
GGTGCTGGCT ACAAGAAATC CAGAAGACAC ACCGTTTCTT GCAACATTTT CGTTAAAAGA 
ACCGCAGATC GTACGTATGC CATTGAGGTT TTCAACAGGT TTAGGGACGG GACTGGTTTG 
GAAACTGATA TGATTTTCTT GAAACCAAGA ATGGAACTGG GAAAGCTTAT CAATGATGCC 
GCATATAATG GGGTGTGGGG CGTTGTTTTA GTTAATAAAA CACACAATGT AGATGTTCAA 
ACTTTCTACA AAGGCTCACA AGGTGAAACG AAATTTGATG AATATATTAG CATATCCGCT 
GATGACGCAG TTGCCATTTT TAATAACATC AAAAACAACA GAAATAATTC TCGTCCTACT 
GATTACCGTG CTATGAGCCA TCAGCAAAAC ATATATGGCG CTCCTCCTCT TCCTGTTCCA 
AACGGCCCAG CTGTCGGACC TCCTCCTCAA ACAAACTATT ACCAGGGTTA CAGTATGCCT 
CCTCCACAAC AACAACAGCA ACAGCCATAT GGTAATTATG GGATGCCACC ACCATCCCAT 
GACCAAGGAT ATGGTTCTCA ACCTCCAATT CCAATGAATC AGAGCTACGG TCGCTACCAG 
ACTTCTATTC CACCACCACC TCCACAACAA CAAATTCCTC AAGGGTATGG TCGTTATCAG 
GCTGGTCCGC CTCCTCAACC ACCTTCTCAA ACTCCAATGG ACCAGCAACA ACTATTATCT 
GCCATTCAAA ACCTTCCACC TAACGTTGTA TCGAATTTGC TTTCAATGGC CCAACAACAG 
CAACAACAAC CTCATGCTCA GCAGCAATTG GTTGGTTTAA TACAATCAAT GCAAGGCCAG 
GCTCCTCAAC AACAGCAACA ACAGTTGGGT GGATATTCCT CTATGAACTC ATCCTCTCCC 
CCTCCTATGA GTACCAATTA CAATGGTCAA AATATATCTG CAAAACCCTC TGCCCCACCA 
ATGTCACACC AACCTCCGCC ACCTCAACAA CAACAACAAC AACAACAACA GCAACAGCAA 
CAGCAACAGC AACCTGCTGG CAATAATGTT CAAAGTCTAT TAGATAGTTT AGCAAAACTA 
CAAAAATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_015134.1
CDS	1..2409
Translation	MSDENHNSDVQDIPSPELSVDSNSNENELMNNSSADDGIEFDAPEEEREAEREEENEEQHELEDVNDEEEEDKEEKGEENGEVINTEEEEEEEHQQKGGNDDDDDDNEEEEEEEEDDDDDDDDDDDDEEEEEEEEEEGNDNSSVGSDSAAEDGEDEEDKKDKTKDKEVELRRETLEKEQKDVDEAIKKITREENDNTHFPTNMENVNYDLLQKQVKYIMDSNMLNLPQFQHLPQEEKMSAILAMLNSNSDTALSVPPHDSTISTTASASATSGARSNDQRKPPLSDAQRRMRFPRADLSKPITEEEHDRYAAYLHGENKITEMHNIPPKSRLFIGNLPLKNVSKEDLFRIFSPYGHIMQINIKNAFGFIQFDNPQSVRDAIECESQEMNFGKKLILEVSSSNARPQFDHGDHGTNSSSTFISSAKRPFQTESGDMYNDDNGAGYKKSRRHTVSCNIFVKRTADRTYAIEVFNRFRDGTGLETDMIFLKPRMELGKLINDAAYNGVWGVVLVNKTHNVDVQTFYKGSQGETKFDEYISISADDAVAIFNNIKNNRNNSRPTDYRAMSHQQNIYGAPPLPVPNGPAVGPPPQTNYYQGYSMPPPQQQQQQPYGNYGMPPPSHDQGYGSQPPIPMNQSYGRYQTSIPPPPPQQQIPQGYGRYQAGPPPQPPSQTPMDQQQLLSAIQNLPPNVVSNLLSMAQQQQQQPHAQQQLVGLIQSMQGQAPQQQQQQLGGYSSMNSSSPPPMSTNYNGQNISAKPSAPPMSHQPPPPQQQQQQQQQQQQQQQQPAGNNVQSLLDSLAKLQK

Target ORF information:

RefSeq Version	NM_001184004.1
Organism	Saccharomyces cerevisiae S288C
Definition	Saccharomyces cerevisiae S288C Nab3p (NAB3), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001184004.1

ORF Insert Sequence:

ATGTCAGATG AAAACCATAA CAGTGATGTT CAAGATATTC CTTCACCTGA ACTATCCGTC 
GATAGTAACT CTAACGAGAA TGAATTGATG AATAACTCAA GCGCAGACGA TGGAATCGAA 
TTTGACGCCC CAGAGGAAGA AAGAGAAGCC GAAAGGGAGG AGGAAAATGA AGAACAACAC 
GAACTGGAAG ATGTGAACGA TGAAGAGGAG GAAGATAAGG AGGAAAAAGG AGAGGAAAAC 
GGGGAAGTAA TAAACACAGA AGAAGAAGAA GAAGAAGAAC ATCAACAAAA AGGCGGAAAT 
GATGATGACG ATGATGATAA TGAAGAGGAA GAAGAGGAAG AAGAGGATGA TGACGATGAT 
GATGATGACG ACGATGATGA TGAAGAAGAA GAAGAAGAAG AAGAAGAAGA AGGCAACGAC 
AACAGTTCGG TAGGCTCAGA TAGTGCCGCT GAAGACGGTG AGGATGAGGA AGACAAAAAG 
GATAAAACCA AAGATAAAGA GGTCGAACTT CGCCGTGAAA CATTGGAAAA AGAACAAAAG 
GACGTAGATG AAGCTATAAA AAAAATAACT CGTGAAGAAA ATGATAATAC TCACTTTCCA 
ACTAATATGG AAAATGTTAA TTACGATCTT TTACAAAAGC AAGTCAAGTA CATTATGGAC 
AGTAACATGC TAAATTTGCC TCAGTTTCAA CATTTACCTC AAGAAGAAAA GATGTCTGCG 
ATTTTAGCAA TGTTAAATTC AAATTCTGAC ACAGCTCTTT CCGTACCTCC TCATGATAGT 
ACTATCTCAA CAACAGCTAG CGCCTCAGCC ACAAGCGGCG CAAGAAGCAA TGATCAAAGA 
AAACCTCCAT TGTCAGATGC CCAAAGACGT ATGAGATTTC CTAGGGCGGA TTTATCTAAG 
CCGATTACCG AAGAAGAACA CGACCGTTAT GCAGCCTATT TGCACGGTGA AAATAAAATC 
ACCGAGATGC ACAATATTCC TCCGAAGTCA AGATTATTCA TTGGTAATTT GCCGCTAAAG 
AACGTTTCTA AGGAGGATTT ATTTAGGATT TTCTCTCCAT ACGGTCATAT CATGCAAATC 
AATATCAAAA ATGCCTTTGG ATTCATTCAG TTTGACAACC CTCAAAGCGT TAGAGATGCA 
ATTGAATGCG AGTCTCAAGA AATGAACTTT GGCAAAAAGT TGATCCTGGA AGTTTCTAGC 
TCGAATGCTC GTCCTCAATT TGATCATGGT GATCACGGTA CAAACAGTAG TTCTACTTTT 
ATTTCTTCCG CAAAACGACC ATTTCAAACT GAATCTGGTG ACATGTACAA TGACGACAAT 
GGTGCTGGCT ACAAGAAATC CAGAAGACAC ACCGTTTCTT GCAACATTTT CGTTAAAAGA 
ACCGCAGATC GTACGTATGC CATTGAGGTT TTCAACAGGT TTAGGGACGG GACTGGTTTG 
GAAACTGATA TGATTTTCTT GAAACCAAGA ATGGAACTGG GAAAGCTTAT CAATGATGCC 
GCATATAATG GGGTGTGGGG CGTTGTTTTA GTTAATAAAA CACACAATGT AGATGTTCAA 
ACTTTCTACA AAGGCTCACA AGGTGAAACG AAATTTGATG AATATATTAG CATATCCGCT 
GATGACGCAG TTGCCATTTT TAATAACATC AAAAACAACA GAAATAATTC TCGTCCTACT 
GATTACCGTG CTATGAGCCA TCAGCAAAAC ATATATGGCG CTCCTCCTCT TCCTGTTCCA 
AACGGCCCAG CTGTCGGACC TCCTCCTCAA ACAAACTATT ACCAGGGTTA CAGTATGCCT 
CCTCCACAAC AACAACAGCA ACAGCCATAT GGTAATTATG GGATGCCACC ACCATCCCAT 
GACCAAGGAT ATGGTTCTCA ACCTCCAATT CCAATGAATC AGAGCTACGG TCGCTACCAG 
ACTTCTATTC CACCACCACC TCCACAACAA CAAATTCCTC AAGGGTATGG TCGTTATCAG 
GCTGGTCCGC CTCCTCAACC ACCTTCTCAA ACTCCAATGG ACCAGCAACA ACTATTATCT 
GCCATTCAAA ACCTTCCACC TAACGTTGTA TCGAATTTGC TTTCAATGGC CCAACAACAG 
CAACAACAAC CTCATGCTCA GCAGCAATTG GTTGGTTTAA TACAATCAAT GCAAGGCCAG 
GCTCCTCAAC AACAGCAACA ACAGTTGGGT GGATATTCCT CTATGAACTC ATCCTCTCCC 
CCTCCTATGA GTACCAATTA CAATGGTCAA AATATATCTG CAAAACCCTC TGCCCCACCA 
ATGTCACACC AACCTCCGCC ACCTCAACAA CAACAACAAC AACAACAACA GCAACAGCAA 
CAGCAACAGC AACCTGCTGG CAATAATGTT CAAAGTCTAT TAGATAGTTT AGCAAAACTA 
CAAAAATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The nucleotide sequence of Saccharomyces cerevisiae chromosome XVI.
Nature387(6632 Suppl)103-5(1997 May)
Bussey H,Storms RK,Ahmed A,Albermann K,Allen E,Ansorge W,Araujo R,Aparicio A,Barrell B,Badcock K,Benes V,Botstein D,Bowman S,Br?ckner M,Carpenter J,Cherry JM,Chung E,Churcher C,Coster F,Davis K,Davis RW,Dietrich FS,Delius H,DiPaolo T,Hani J

Life with 6000 genes.
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)
Goffeau A,Barrell BG,Bussey H,Davis RW,Dujon B,Feldmann H,Galibert F,Hoheisel JD,Jacq C,Johnston M,Louis EJ,Mewes HW,Murakami Y,Philippsen P,Tettelin H,Oliver SG

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NAB3 cDNA ORF clone, Saccharomyces cerevisiae S288C

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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