POP2 cDNA ORF clone, Saccharomyces cerevisiae S288C

Gene
Clones
gRNAs

Summary

Gene Symbol	POP2
Entrez Gene ID	855788
Full Name	CCR4-NOT core DEDD family RNase subunit POP2
Synonyms	CAF1
Gene Type	protein-coding
Organism	Saccharomyces cerevisiae S288C

mRNA and Protein(s)

mRNA	Protein	Name
NM_001183229.3	NP_014450.3	CCR4-NOT core DEDD family RNase subunit POP2

Pathways from BioSystems

Homologies

Saccharomyces cerevisiae (baker's yeast)	POP2	NP_014450.3
Kluyveromyces lactis	KLLA0C18821g	XP_453039.1
Eremothecium gossypii	AGOS_ABR119C	NP_983066.2

Gene Ontology
Bibliography

Related articles in PubMed

The nucleotide sequence of Saccharomyces cerevisiae chromosome XIV and its evolutionary implications.
Philippsen P, Kleine K, P?hlmann R, D?sterh?ft A, Hamberg K, Hegemann JH, Obermaier B, Urrestarazu LA, Aert R, Albermann K, Altmann R, Andr? B, Baladron V, Ballesta JP, B?cam AM, Beinhauer J, Boskovic J, Buitrago MJ, Bussereau F, Coster F, Crouzet M, D'Angelo M, Dal Pero F, De Antoni A, Del Rey F, Doignon F, Domdey H, Dubois E, Fiedler T, Fleig U, Floeth M, Fritz C, Gaillardin C, Garcia-Cantalejo JM, Glansdorff NN, Goffeau A, Gueldener U, Herbert C, Heumann K, Heuss-Neitzel D, Hilbert H, Hinni K, Iraqui Houssaini I, Jacquet M, Jimenez A, Jonniaux JL, Karpfinger L, Lanfranchi G, Lepingle A, Levesque H, Lyck R, Maftahi M, Mallet L, Maurer KC, Messenguy F, Mewes HW, M?sti D, Nasr F, Nicaud JM, Niedenthal RK, Pandolfo D, Pi?rard A, Piravandi E, Planta RJ, Pohl TM, Purnelle B, Rebischung C, Remacha M, Revuelta JL, Rinke M, Saiz JE, Sartorello F, Scherens B, Sen-Gupta M, Soler-Mira A, Urbanus JH, Valle G, Van Dyck L, Verhasselt P, Vierendeels F, Vissers S, Voet M, Volckaert G, Wach A, Wambutt R, Wedler H, Zollner A, Hani J
Nature387(6632 Suppl)93-8(1997 May)

Life with 6000 genes.
Goffeau A, Barrell BG, Bussey H, Davis RW, Dujon B, Feldmann H, Galibert F, Hoheisel JD, Jacq C, Johnston M, Louis EJ, Mewes HW, Murakami Y, Philippsen P, Tettelin H, Oliver SG
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Here, the authors report that yeast chromatin assembly factor 1 (CAF1), a conserved histone chaperone complex that deposits H3-H4 during DNA replication, binds a single H3-H4 heterodimer in solution.
Title: Insights into the molecular architecture and histone H3-H4 deposition mechanism of yeast Chromatin assembly factor 1.

The authors show that yeast chromatin assembly factor 1 binding to a histone H3-H4 dimer activates the Cac1 winged helix domain interaction with DNA. This drives the formation of a transient intermediate containing two chromatin assembly factor 1 complexes, each associated with one H3-H4 dimer. Here, the (H3-H4)2 tetramer is formed and deposited onto DNA.
Title: DNA-mediated association of two histone-bound complexes of yeast Chromatin Assembly Factor-1 (CAF-1) drives tetrasome assembly in the wake of DNA replication.

MIF4G domain positioned centrally within the Not1 protein recognizes Caf1, which in turn binds the LRR domain of Ccr4 and tethers the Ccr4 nuclease domain
Title: Architecture of the nuclease module of the yeast Ccr4-not complex: the Not1-Caf1-Ccr4 interaction.

tight connection between DNA synthesis and histone deposition during DNA replication mediated by H3K56ac/CAF1/Rtt106 provides a mechanism for the stabilization of advancing replication forks and the maintenance of genome integrity.
Title: Histone H3K56 acetylation, CAF1, and Rtt106 coordinate nucleosome assembly and stability of advancing replication forks.

deletion of POP2 is accompanied by an increase of both SLT2 and KDX1 levels.
Title: Comparative transcriptome analysis of yeast strains carrying slt2, rlm1, and pop2 deletions.

The following POP2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the POP2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OSi04676	NM_001183229.3 Latest version!	Saccharomyces cerevisiae S288C CCR4-NOT core DEDD family RNase subunit POP2 (POP2), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$258.30 $369.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

POP2 ( NM_001183229.3 ) cDNA ORF clone, Saccharomyces cerevisiae S288C -> NP_014450.3 Saccharomyces cerevisiae S288C CCR4-NOT core DEDD family RNase subunit POP2 (POP2), partial mRNA.

Price & Availability↑

CloneID	OSi04676
Clone ID Related Accession (Same CDS sequence)	NM_001183229.3
Accession Version	NM_001183229.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1302bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-31
Organism	Saccharomyces cerevisiae S288C
Product	CCR4-NOT core DEDD family RNase subunit POP2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by SGD. This record is derived from an annotated genomic sequence (NC_001146). On Jul 30, 2012 this sequence version replaced NM_001183229.2. ##Genome-Annotation-Data-START## Annotation Provider :: SGD Annotation Status :: Full Annotation Annotation Version :: R64-2-1 URL :: http://www.yeastgenome.org/ ##Genome-Annotation-Data-END## COMPLETENESS: incomplete on both ends.

ATGCAATCTA TGAATGTACA ACCGAGGGTA TTAGCTGTAG GAGGTGAGCA GTTTTTCTCT 
CAGAGGCAGG CTTCTGAACA ACACCAGCAG CAAAACATGG GACCTCAGGT GTATTCGCCA 
AAGGTTAATA GAGCAAGAAT GTTTCCTCAA GGGATGCCTG TAAATACAAT TAATGGCTCT 
GTTAATCAGG AGATGAACAA TGCCTATCTT TTGAAGCAGA AGAACGAGCC ACTGTTAACT 
CAGCAACAAC AGCAACAGCA ACAACAACAG CAGCCATTTA ATATAGGAAC GCCTGTTTCG 
GTGGCTAGTT TGCCGCCCGG ACTGAACGTT CTGCAGCAAC AACAACAGCA GCAACAACAA 
CAGCAGCAAC AGCAGCAGGG AGTGGGACTC AATCGTCCCT TGGCTTCCCA GCTACCCAAG 
CATTTAACCA ACCAAAGTAT GCCACCGATC TTTTTACCAC CCCCAAATTA TCTTTTCGTT 
CGCGATGTCT GGAAGTCCAA CCTTTACAGT GAATTCGCCG TTATAAGACA ACTGGTCTCG 
CAGTATAATC ATGTGTCAAT CAGTACTGAG TTTGTGGGGA CTTTGGCTAG GCCGATCGGC 
ACTTTCAGGT CGAAGGTCGA TTACCACTAT CAGACAATGA GGGCCAACGT AGACTTCTTA 
AACCCAATAC AACTTGGTCT TTCCTTGAGT GACGCCAACG GCAATAAGCC TGACAACGGT 
CCCTCAACGT GGCAGTTCAA TTTTGAATTT GACCCAAAGA AGGAAATCAT GTCCACAGAA 
TCGTTAGAAT TACTCAGAAA ATCAGGTATC AACTTCGAAA AACACGAAAA TTTAGGCATC 
GATGTATTTG AATTTTCGCA GCTTCTAATG GACTCTGGTT TAATGATGGA TGATTCTGTT 
ACTTGGATCA CATACCATGC GGCATATGAT CTAGGTTTCC TGATCAACAT TCTAATGAAT 
GACTCCATGC CCAACAACAA GGAGGATTTC GAGTGGTGGG TCCATCAATA CATGCCCAAT 
TTCTACGACT TAAACCTGGT GTATAAAATA ATCCAGGAGT TCAAGAACCC TCAATTGCAA 
CAATCTTCAC AACAGCAACA GCAGCAGCAA TATTCACTAA CCACACTTGC CGATGAGTTA 
GGGCTACCAA GATTCTCTAT ATTTACTACT ACAGGCGGCC AGAGTCTATT GATGCTTCTA 
TCCTTCTGCC AATTGAGCAA GCTGTCTATG CATAAGTTCC CAAACGGTAC GGATTTCGCC 
AAGTACCAAG GTGTCATATA CGGTATTGAT GGGGACCAAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_014450.3
CDS	1..1302
Translation	MQSMNVQPRVLAVGGEQFFSQRQASEQHQQQNMGPQVYSPKVNRARMFPQGMPVNTINGSVNQEMNNAYLLKQKNEPLLTQQQQQQQQQQQPFNIGTPVSVASLPPGLNVLQQQQQQQQQQQQQQQGVGLNRPLASQLPKHLTNQSMPPIFLPPPNYLFVRDVWKSNLYSEFAVIRQLVSQYNHVSISTEFVGTLARPIGTFRSKVDYHYQTMRANVDFLNPIQLGLSLSDANGNKPDNGPSTWQFNFEFDPKKEIMSTESLELLRKSGINFEKHENLGIDVFEFSQLLMDSGLMMDDSVTWITYHAAYDLGFLINILMNDSMPNNKEDFEWWVHQYMPNFYDLNLVYKIIQEFKNPQLQQSSQQQQQQQYSLTTLADELGLPRFSIFTTTGGQSLLMLLSFCQLSKLSMHKFPNGTDFAKYQGVIYGIDGDQ

Target ORF information:

RefSeq Version	NM_001183229.3
Organism	Saccharomyces cerevisiae S288C
Definition	Saccharomyces cerevisiae S288C CCR4-NOT core DEDD family RNase subunit POP2 (POP2), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001183229.3

ORF Insert Sequence:

ATGCAATCTA TGAATGTACA ACCGAGGGTA TTAGCTGTAG GAGGTGAGCA GTTTTTCTCT 
CAGAGGCAGG CTTCTGAACA ACACCAGCAG CAAAACATGG GACCTCAGGT GTATTCGCCA 
AAGGTTAATA GAGCAAGAAT GTTTCCTCAA GGGATGCCTG TAAATACAAT TAATGGCTCT 
GTTAATCAGG AGATGAACAA TGCCTATCTT TTGAAGCAGA AGAACGAGCC ACTGTTAACT 
CAGCAACAAC AGCAACAGCA ACAACAACAG CAGCCATTTA ATATAGGAAC GCCTGTTTCG 
GTGGCTAGTT TGCCGCCCGG ACTGAACGTT CTGCAGCAAC AACAACAGCA GCAACAACAA 
CAGCAGCAAC AGCAGCAGGG AGTGGGACTC AATCGTCCCT TGGCTTCCCA GCTACCCAAG 
CATTTAACCA ACCAAAGTAT GCCACCGATC TTTTTACCAC CCCCAAATTA TCTTTTCGTT 
CGCGATGTCT GGAAGTCCAA CCTTTACAGT GAATTCGCCG TTATAAGACA ACTGGTCTCG 
CAGTATAATC ATGTGTCAAT CAGTACTGAG TTTGTGGGGA CTTTGGCTAG GCCGATCGGC 
ACTTTCAGGT CGAAGGTCGA TTACCACTAT CAGACAATGA GGGCCAACGT AGACTTCTTA 
AACCCAATAC AACTTGGTCT TTCCTTGAGT GACGCCAACG GCAATAAGCC TGACAACGGT 
CCCTCAACGT GGCAGTTCAA TTTTGAATTT GACCCAAAGA AGGAAATCAT GTCCACAGAA 
TCGTTAGAAT TACTCAGAAA ATCAGGTATC AACTTCGAAA AACACGAAAA TTTAGGCATC 
GATGTATTTG AATTTTCGCA GCTTCTAATG GACTCTGGTT TAATGATGGA TGATTCTGTT 
ACTTGGATCA CATACCATGC GGCATATGAT CTAGGTTTCC TGATCAACAT TCTAATGAAT 
GACTCCATGC CCAACAACAA GGAGGATTTC GAGTGGTGGG TCCATCAATA CATGCCCAAT 
TTCTACGACT TAAACCTGGT GTATAAAATA ATCCAGGAGT TCAAGAACCC TCAATTGCAA 
CAATCTTCAC AACAGCAACA GCAGCAGCAA TATTCACTAA CCACACTTGC CGATGAGTTA 
GGGCTACCAA GATTCTCTAT ATTTACTACT ACAGGCGGCC AGAGTCTATT GATGCTTCTA 
TCCTTCTGCC AATTGAGCAA GCTGTCTATG CATAAGTTCC CAAACGGTAC GGATTTCGCC 
AAGTACCAAG GTGTCATATA CGGTATTGAT GGGGACCAAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The nucleotide sequence of Saccharomyces cerevisiae chromosome XIV and its evolutionary implications.
Nature387(6632 Suppl)93-8(1997 May)
Philippsen P,Kleine K,P?hlmann R,D?sterh?ft A,Hamberg K,Hegemann JH,Obermaier B,Urrestarazu LA,Aert R,Albermann K,Altmann R,Andr? B,Baladron V,Ballesta JP,B?cam AM,Beinhauer J,Boskovic J,Buitrago MJ,Bussereau F,Coster F,Crouzet M,D'Angelo M,Dal Pero F,De Antoni A,Del Rey F,Doignon F,Domdey H,Dubois E,Fiedler T,Fleig U,Floeth M,Fritz C,Gaillardin C,Garcia-Cantalejo JM,Glansdorff NN,Goffeau A,Gueldener U,Herbert C,Heumann K,Heuss-Neitzel D,Hilbert H,Hinni K,Iraqui Houssaini I,Jacquet M,Jimenez A,Jonniaux JL,Karpfinger L,Lanfranchi G,Lepingle A,Levesque H,Lyck R,Maftahi M,Mallet L,Maurer KC,Messenguy F,Mewes HW,M?sti D,Nasr F,Nicaud JM,Niedenthal RK,Pandolfo D,Pi?rard A,Piravandi E,Planta RJ,Pohl TM,Purnelle B,Rebischung C,Remacha M,Revuelta JL,Rinke M,Saiz JE,Sartorello F,Scherens B,Sen-Gupta M,Soler-Mira A,Urbanus JH,Valle G,Van Dyck L,Verhasselt P,Vierendeels F,Vissers S,Voet M,Volckaert G,Wach A,Wambutt R,Wedler H,Zollner A,Hani J

Life with 6000 genes.
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)
Goffeau A,Barrell BG,Bussey H,Davis RW,Dujon B,Feldmann H,Galibert F,Hoheisel JD,Jacq C,Johnston M,Louis EJ,Mewes HW,Murakami Y,Philippsen P,Tettelin H,Oliver SG

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POP2 cDNA ORF clone, Saccharomyces cerevisiae S288C

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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