SSK2 cDNA ORF clone, Saccharomyces cerevisiae S288C

Gene
Clones
gRNAs

Summary

Gene Symbol	SSK2
Entrez Gene ID	855765
Full Name	mitogen-activated protein kinase kinase kinase SSK2
Gene Type	protein-coding
Organism	Saccharomyces cerevisiae S288C

mRNA and Protein(s)

mRNA	Protein	Name
NM_001183208.1	NP_014428.1	mitogen-activated protein kinase kinase kinase SSK2

Pathways from BioSystems

Homologies

Saccharomyces cerevisiae (baker's yeast)	SSK22	NP_009998.2
Saccharomyces cerevisiae (baker's yeast)	SSK2	NP_014428.1
Eremothecium gossypii	AGOS_AER264C	NP_985121.2
Schizosaccharomyces pombe (fission yeast)	wis4	NP_593557.1
Kluyveromyces lactis	KLLA0C08525g	XP_452580.1
Neurospora crassa	NCU03071	XP_964164.1
Magnaporthe oryzae (rice blast fungus)	MGG_00183	XP_003718937.1

Gene Ontology
Bibliography

Related articles in PubMed

The nucleotide sequence of Saccharomyces cerevisiae chromosome XIV and its evolutionary implications.
Philippsen P, Kleine K, P?hlmann R, D?sterh?ft A, Hamberg K, Hegemann JH, Obermaier B, Urrestarazu LA, Aert R, Albermann K, Altmann R, Andr? B, Baladron V, Ballesta JP, B?cam AM, Beinhauer J, Boskovic J, Buitrago MJ, Bussereau F, Coster F, Crouzet M, D'Angelo M, Dal Pero F, De Antoni A, Del Rey F, Doignon F, Domdey H, Dubois E, Fiedler T, Fleig U, Floeth M, Fritz C, Gaillardin C, Garcia-Cantalejo JM, Glansdorff NN, Goffeau A, Gueldener U, Herbert C, Heumann K, Heuss-Neitzel D, Hilbert H, Hinni K, Iraqui Houssaini I, Jacquet M, Jimenez A, Jonniaux JL, Karpfinger L, Lanfranchi G, Lepingle A, Levesque H, Lyck R, Maftahi M, Mallet L, Maurer KC, Messenguy F, Mewes HW, M?sti D, Nasr F, Nicaud JM, Niedenthal RK, Pandolfo D, Pi?rard A, Piravandi E, Planta RJ, Pohl TM, Purnelle B, Rebischung C, Remacha M, Revuelta JL, Rinke M, Saiz JE, Sartorello F, Scherens B, Sen-Gupta M, Soler-Mira A, Urbanus JH, Valle G, Van Dyck L, Verhasselt P, Vierendeels F, Vissers S, Voet M, Volckaert G, Wach A, Wambutt R, Wedler H, Zollner A, Hani J
Nature387(6632 Suppl)93-8(1997 May)

Life with 6000 genes.
Goffeau A, Barrell BG, Bussey H, Davis RW, Dujon B, Feldmann H, Galibert F, Hoheisel JD, Jacq C, Johnston M, Louis EJ, Mewes HW, Murakami Y, Philippsen P, Tettelin H, Oliver SG
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Cadmium-induced HOG activation is dependent on the MAP kinase kinase kinase Ssk2, but not its paralog Ssk22, in the Sln1 branch.
Title: Cadmium-induced activation of high osmolarity glycerol pathway through its Sln1 branch is dependent on the MAP kinase kinase kinase Ssk2, but not its paralog Ssk22, in budding yeast.

The osmotic stress response through the Ssk1p-independent Ssk2p activation is strong, although its duration is short compared with the Ssk1p-dependent activation.
Title: Ssk1p-independent activation of Ssk2p plays an important role in the osmotic stress response in Saccharomyces cerevisiae: alternative activation of Ssk2p in osmotic stress.

Data discovered that six non-essential protein kinase (PKase) disruptions (Deltabck1, Deltamkk1, Deltaslt2/Deltampk1, Deltamck1, Deltassk2 and Deltayak1) suppressed the Cas-phenotype of the Deltaptp2 Deltamsg5 double disruptant.
Title: Identification of protein kinase disruptions as suppressors of the calcium sensitivity of S. cerevisiae Deltaptp2 Deltamsg5 protein phosphatase double disruptant.

Constitutive activation of Ssk2 occurs when Ssk1 phosphorylation is blocked by either an Ssk1 mutation at the phosphorylation site or an Ssk1 mutation that inhibits its interaction with Ypd1, the donor of phosphate to Ssk1.
Title: Phosphorylated Ssk1 prevents unphosphorylated Ssk1 from activating the Ssk2 mitogen-activated protein kinase kinase kinase in the yeast high-osmolarity glycerol osmoregulatory pathway.

Ssk2p acts downstream of Bud6p and Pea2p and upstream of tropomyosin to drive actin recovery, possibly by upregulating the actin nucleation activity of the formins.
Title: Requirement for the polarisome and formin function in Ssk2p-mediated actin recovery from osmotic stress in Saccharomyces cerevisiae.

The following SSK2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the SSK2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OSi04654	NM_001183208.1 Latest version!	Saccharomyces cerevisiae S288C mitogen-activated protein kinase kinase kinase SSK2 (SSK2), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

SSK2 ( NM_001183208.1 ) cDNA ORF clone, Saccharomyces cerevisiae S288C -> NP_014428.1 Saccharomyces cerevisiae S288C mitogen-activated protein kinase kinase kinase SSK2 (SSK2), partial mRNA.

Price & Availability↑

CloneID	OSi04654
Clone ID Related Accession (Same CDS sequence)	NM_001183208.1
Accession Version	NM_001183208.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4740bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-31
Organism	Saccharomyces cerevisiae S288C
Product	mitogen-activated protein kinase kinase kinase SSK2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by SGD. This record is derived from an annotated genomic sequence (NC_001146). ##Genome-Annotation-Data-START## Annotation Provider :: SGD Annotation Status :: Full Annotation Annotation Version :: R64-2-1 URL :: http://www.yeastgenome.org/ ##Genome-Annotation-Data-END## COMPLETENESS: incomplete on both ends.

ATGTCGCATT CAGACTACTT CAATTATAAG CCTTACGGCG ATTCCACGGA AAAGCCCAGC 
AGCTCCAAGA TGAGGCAGTC ATCTTCATCA TCTTCATCTA GACTAAGGTC GGAAAGTTTA 
GGGCGAAATT CCAATACTAC GCAGGCTCGA GTAGCGTCAT CGCCCATCAG CCCAGGGCTG 
CATTCTACCC AGTACTTTAG ATCACCAAAC GCTGTTTACA GCCCTGGAGA GTCTCCATTA 
AATACAGTAC AGCTATTCAA TCGTCTTCCG GGTATACCTC AAGGTCAGTT TTTTCATCAA 
AATGCCATTT CTGGGTCCTC CAGCTCCTCG GCAAGATCTA GTAGACGGCC CTCGAATATT 
GGTCTTCCTC TACCAAAAAA TCCTCAGCAG TCTCTACCGA AGTTGTCTAC TCAACCTGTT 
CCTGTACATA AGAAAGTTGA GGCTAGTAAA ACAGAGAGCG AGATTATTAA GAAGCCCGCT 
CCCGTGAATA GTAATCAAGA TCCATTATTG ACGACCCCAA CGTTAGTGAT ATCACCAGAA 
CTGGCTTCAC TAAATACAAC GAATACATCG ATTATGTCAA CGCCACAAAA TATTACAAAC 
CAAACTTCGA ACAAACACAT TCCCACCAGA TCGCAACCAA ATGGGTCAAC AAGTTCCTCC 
ACTTTGCAGG ATATTGTCAC GACAAATAGC TCGCAACGGT CTGTAGGCCA CCATGGTGGA 
AGCACAACGA GCCTCCGAAC ATACAAAAAA CAATATGTAT TAAATGAACA GTTATATTTA 
AGAAAAATGA GAAACCGTGC TAATGATGAT TATTACACTA GAGGTATAGT CGCATCATCC 
AACTTTGAAG ATGACGAAGA AAATTTTAGT AACAAAGGTG AAGATGACTT AGAACTAGAA 
ATGGATGATC TTTTAAAGGT AGAAGGTGAG GATAAAGATA ACGACTTCAA TTTTGGTTAT 
AATTTTATTA CGTCGAGCAC AAAAAATAAT GAAAATGTTG TTTCGATGAG CCTAAATTAT 
CTAAAGGGCA AATTGGATTG GTTGAGGGAT GTGAACAATG ATCAACCGTG TGAAATAGAA 
GATGAGGAGT GGCATTCCAT ACTGGGGAGC GAGGATTTGC TGTCAAAATT GTTACAAAAT 
CCTATGGTGA ACAACCGATT TGAATGGCAA ACAATGTTAT CTAAGGTACT GAAGGGAGAT 
ATTGTGAGGA ATGAAAAAAC GAAGATTGCA AATCAAGGGA AAGGCCCTGG CTTCAATACT 
CAGTTTTCAG ATGACATTTG GATTGAGTTG AAGGCATGGA TGAATGGGAG GACCGTGGAA 
GATCAGAACA AATCTCTGAG GATTTTTAGG GATTCTACTG ACTCCGTATT TCAAGAAATC 
ATGGCCTTTA AACTAGAAGA TAATATGAGC GCTGACGAGG CTGCAGAGAC TATCAAATCA 
CTAGTAGACA AATATTATAG AGTCTTAAAT CTATGGCCTA ACATTAAAAG AATGCATGCT 
GAAAAACCCA TTACTAAAAC AGAAGCATTC AGGAATCGAA TAGATACTTT GAATAGTTGG 
CTTAATTTCA AATTTAACTT TGATACTAAT ATTGCGTACC TGAAAAAATG GATAGTTGGC 
AATAAAGAGC TAGAAAGCAC TACCGAAGTG GATAACACCA CCGTGAATTT GGATGATCCA 
GCCGTTTTCG CCACTAATTG TAAACGCTTT GCGGAGCAAA TTATGAAGGA AAAGGATATT 
GAACTGATAT TTCAAAAAAA AATATTCTTT CCATTAGCAC CATGGATTTT GAAGGCCAAA 
TTTTTCTTCT TGAAATACCA AAAAACTTGG AATGAATTGA ATCTATCTTA TTTGGATCAA 
GATCTGGAAT TTTTATTGAT GTTTCCAATG CGTTTGGTAA AAGATATAAT ACTAATTCGC 
CTATCTTACG CGAAGAAAAT ACAGAATCCA ACCTTGATGA TGATCGATCA AATGATGGAC 
GATTTTAGTA CATATATTAA GTTGGCAGTC CAAATGAAAT TCACTGTTGC TTCTTACTGT 
AATGACTGGT TTTTTAAAGT GAAAATCGAT CCCGAATTTG ATCATACCGT TGTTGAAGGA 
TTGGAATATT TTTTCTCCAT TTTGGAACTG AGAATATTAT ATAGTGGCAA AAACTCATTC 
AAGACTTCTA AAGAACCTGA TCTGTTATTA AAATATTGGG AAATGTTTAG AAACGTCGGC 
TATTATATTG ATGATGCAGG CGAACTGATC GCAGCAGAAT TTACGAAGTT GACACTTAGA 
TTGGTTCACA GATTGCACGC TTACCTTTTA AGGCAGCAAA ACACTCCGCC AAAATTAGAG 
AATGAAGCTG CTGCTGAAAA ATGGCTGGTG CAAATATTCG AAATACTTGG ATCCATGAAA 
AGAAAACTCA ATAGATTCAC CAATATTTTG ACAAAAGCAT TTCAAAATTT TGTCCGCTAC 
AAGATAGAAG ATCACAACTA TCTGCTAAAG CAACTAAAAG AAACAGGCCA CTTTCTTATA 
TACACAGGAG GTTACCTAGA GCAAAATGGT ACTTATTTAA TTGGTAGCCC AGAGCTATTA 
GGCTGTAAAG ATGATGATAT TTTAAGAATC ATTAAGAATT CAGACATCGG TTGTGATTTA 
GTGCCTAAGC TAGAAATTAA TAACAGTCTG ACAATTTATA ATGCTTTGGA TGATAATTGG 
AACTCCAACT CATCACTGGG TTCAGATATC TCGAATGATG GTACCCCATT TTATTATATC 
AAAAACGATT TGACCACCCA GCCTAGATCT TATAACGGTA ATAGAGTCAA TCGTGAACCG 
GATTTTGAAA ACAGCAGGAG CACGGAGGAA GAGTTTTATG AACTGGAGAC AAGATTGAAC 
TCTCTTGGTT ATGTATTGGT ATTGACTCCG CAAGAGCCAT TACTTTGGGA GGGTGAGATG 
TATAATCTAT CCGATAACAA AACAATTAAA CCAGAGGGAT TGAACTTGAA AGTAATTCCT 
AATTCAATAG ATTTGATGTG CCAAGGATCC AGTTATGCCT TAGAATACCA ATGTGACAGG 
TTCCAACAGA TATCTGGTAG CTCAGTTTCT TTCTTGGAAA AAAAATCTTC CTCAGAAACG 
GTTAAAAACA ACTTACAAAG GATAAATAAA GCATATTTCA GATGCACGTA CAGTGTTCTG 
AAGAACTATA CAAAGATTGT GACCACGTTC AAGAAGGTAA GTCCTGTCAA TGATTTATTG 
AATAATATTT TCCTTTTTGG GAGGGATTTT GGTCTGAACT TTTTGAGAAT TAATGTTGCC 
AACAATGAAA AAAGATCCAT TATAATACTT TTAATGATGC GGTTAAGTAT CGGATGGCTG 
AAGTTCTTAG CTGAAGACTG TGATCCGACT GATCAAAGGG TATTTAGGTG GTGTGTCACG 
TCAATGGAGT TCGCGATGCA TATGGTAAGC GGTTGGAACA TACTAGCTCT TGATGAATGC 
CAATTTTCTT CTTTAAAACA AAAAATTTCA GAATGCATGT CATTACTTAT TTCTCATTTT 
GATATAATAG GCGCACGCTC CATAGAAGTT GAGAAAATCA ATCAACAAGC TAGATCAAAT 
CTTGATTTGG AAGATGTGTT TGACGATGAT ATGATGCTAC AAGTGAATTC CGAGTTCCGA 
GTACAAAGTA TAATGGAATT GGAAGAAAGG ATAAAGCGGA ATCCTCATCA AACTGGTAAA 
GTAATTGATG ATAGTGACAA AGGTAACAAG TACCTTGTTT CTTTGGCATC TTCCATATCG 
AACGTTTCTA TGAGATGGCA AAAGAGGAAC TTTATTGGTG GCGGTACTTT TGGAAGGGTA 
TATTCTGCTG TTGATTTGGA TAATGGTGAG ATTTTAGCAG TCAAGGAAAT CAATATTCAA 
GATAGCAAAT CAATGCAAAA AATATTCCCC TTAATCAAGG AGGAAATGAG TGTCTTAGAG 
ATATTGAACC ATCCAAATAT AGTTTCATAT TACGGTGTTG AAGTTCATCG TGATAAAGTT 
AACATCTTTA TGGAATATTG TGAAGGCGGT TCCCTAGCAG CTCTTTTGGA GCATGGTCGT 
ATTGAAGATG AAATGGTCAC TCAAGTCTAC ACTTTACAAT TGCTAGAAGG ACTTGCATAT 
TTGCATGAAT CCGGCATTGT TCACCGTGAT GTTAAACCCG AAAACATCCT ACTAGATTTT 
AATGGTGTTA TTAAGTATGT TGATTTTGGT GCTGCTAAAA AAATTGCTAA TAATGGAACT 
AGATTGGCAA GTATGAACAA AATCGAAAAC GCAGATGGTG AACACGAAGA TGTTACCCAT 
GTTTCTGATT CAAAGGCAGT GAAAAATAAC GAAAATGCTC TATTAGACAT GATGGGAACT 
CCTATGTACA TGGCTCCAGA ATCCATCACT GGATCTACCA CCAAAGGCAA ACTTGGGGCA 
GACGATGTTT GGTCGTTAGG CTGCGTGGTA TTAGAAATGA TCACTGGTAG ACGGCCATGG 
GCTAACTTAG ATAATGAATG GGCTATCATG TACCATGTTG CTGCAGGACA TACCCCACAA 
TTCCCTACTA AGGATGAAGT GTCGTCTGCT GGTATGAAAT TTCTGGAAAG ATGTCTTATT 
CAAAACCCCT CCAAGAGAGC CAGTGCGGTT GAGCTGTTGA TGGATCCTTG GATTGTACAA 
ATTAGAGAAA TAGCCTTTGG CGACGATTCT TCCTCTACAG ATACTGAGGA AAGAGAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_014428.1
CDS	1..4740
Translation	MSHSDYFNYKPYGDSTEKPSSSKMRQSSSSSSSRLRSESLGRNSNTTQARVASSPISPGLHSTQYFRSPNAVYSPGESPLNTVQLFNRLPGIPQGQFFHQNAISGSSSSSARSSRRPSNIGLPLPKNPQQSLPKLSTQPVPVHKKVEASKTESEIIKKPAPVNSNQDPLLTTPTLVISPELASLNTTNTSIMSTPQNITNQTSNKHIPTRSQPNGSTSSSTLQDIVTTNSSQRSVGHHGGSTTSLRTYKKQYVLNEQLYLRKMRNRANDDYYTRGIVASSNFEDDEENFSNKGEDDLELEMDDLLKVEGEDKDNDFNFGYNFITSSTKNNENVVSMSLNYLKGKLDWLRDVNNDQPCEIEDEEWHSILGSEDLLSKLLQNPMVNNRFEWQTMLSKVLKGDIVRNEKTKIANQGKGPGFNTQFSDDIWIELKAWMNGRTVEDQNKSLRIFRDSTDSVFQEIMAFKLEDNMSADEAAETIKSLVDKYYRVLNLWPNIKRMHAEKPITKTEAFRNRIDTLNSWLNFKFNFDTNIAYLKKWIVGNKELESTTEVDNTTVNLDDPAVFATNCKRFAEQIMKEKDIELIFQKKIFFPLAPWILKAKFFFLKYQKTWNELNLSYLDQDLEFLLMFPMRLVKDIILIRLSYAKKIQNPTLMMIDQMMDDFSTYIKLAVQMKFTVASYCNDWFFKVKIDPEFDHTVVEGLEYFFSILELRILYSGKNSFKTSKEPDLLLKYWEMFRNVGYYIDDAGELIAAEFTKLTLRLVHRLHAYLLRQQNTPPKLENEAAAEKWLVQIFEILGSMKRKLNRFTNILTKAFQNFVRYKIEDHNYLLKQLKETGHFLIYTGGYLEQNGTYLIGSPELLGCKDDDILRIIKNSDIGCDLVPKLEINNSLTIYNALDDNWNSNSSLGSDISNDGTPFYYIKNDLTTQPRSYNGNRVNREPDFENSRSTEEEFYELETRLNSLGYVLVLTPQEPLLWEGEMYNLSDNKTIKPEGLNLKVIPNSIDLMCQGSSYALEYQCDRFQQISGSSVSFLEKKSSSETVKNNLQRINKAYFRCTYSVLKNYTKIVTTFKKVSPVNDLLNNIFLFGRDFGLNFLRINVANNEKRSIIILLMMRLSIGWLKFLAEDCDPTDQRVFRWCVTSMEFAMHMVSGWNILALDECQFSSLKQKISECMSLLISHFDIIGARSIEVEKINQQARSNLDLEDVFDDDMMLQVNSEFRVQSIMELEERIKRNPHQTGKVIDDSDKGNKYLVSLASSISNVSMRWQKRNFIGGGTFGRVYSAVDLDNGEILAVKEINIQDSKSMQKIFPLIKEEMSVLEILNHPNIVSYYGVEVHRDKVNIFMEYCEGGSLAALLEHGRIEDEMVTQVYTLQLLEGLAYLHESGIVHRDVKPENILLDFNGVIKYVDFGAAKKIANNGTRLASMNKIENADGEHEDVTHVSDSKAVKNNENALLDMMGTPMYMAPESITGSTTKGKLGADDVWSLGCVVLEMITGRRPWANLDNEWAIMYHVAAGHTPQFPTKDEVSSAGMKFLERCLIQNPSKRASAVELLMDPWIVQIREIAFGDDSSSTDTEERE

Target ORF information:

RefSeq Version	NM_001183208.1
Organism	Saccharomyces cerevisiae S288C
Definition	Saccharomyces cerevisiae S288C mitogen-activated protein kinase kinase kinase SSK2 (SSK2), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001183208.1

ORF Insert Sequence:

ATGTCGCATT CAGACTACTT CAATTATAAG CCTTACGGCG ATTCCACGGA AAAGCCCAGC 
AGCTCCAAGA TGAGGCAGTC ATCTTCATCA TCTTCATCTA GACTAAGGTC GGAAAGTTTA 
GGGCGAAATT CCAATACTAC GCAGGCTCGA GTAGCGTCAT CGCCCATCAG CCCAGGGCTG 
CATTCTACCC AGTACTTTAG ATCACCAAAC GCTGTTTACA GCCCTGGAGA GTCTCCATTA 
AATACAGTAC AGCTATTCAA TCGTCTTCCG GGTATACCTC AAGGTCAGTT TTTTCATCAA 
AATGCCATTT CTGGGTCCTC CAGCTCCTCG GCAAGATCTA GTAGACGGCC CTCGAATATT 
GGTCTTCCTC TACCAAAAAA TCCTCAGCAG TCTCTACCGA AGTTGTCTAC TCAACCTGTT 
CCTGTACATA AGAAAGTTGA GGCTAGTAAA ACAGAGAGCG AGATTATTAA GAAGCCCGCT 
CCCGTGAATA GTAATCAAGA TCCATTATTG ACGACCCCAA CGTTAGTGAT ATCACCAGAA 
CTGGCTTCAC TAAATACAAC GAATACATCG ATTATGTCAA CGCCACAAAA TATTACAAAC 
CAAACTTCGA ACAAACACAT TCCCACCAGA TCGCAACCAA ATGGGTCAAC AAGTTCCTCC 
ACTTTGCAGG ATATTGTCAC GACAAATAGC TCGCAACGGT CTGTAGGCCA CCATGGTGGA 
AGCACAACGA GCCTCCGAAC ATACAAAAAA CAATATGTAT TAAATGAACA GTTATATTTA 
AGAAAAATGA GAAACCGTGC TAATGATGAT TATTACACTA GAGGTATAGT CGCATCATCC 
AACTTTGAAG ATGACGAAGA AAATTTTAGT AACAAAGGTG AAGATGACTT AGAACTAGAA 
ATGGATGATC TTTTAAAGGT AGAAGGTGAG GATAAAGATA ACGACTTCAA TTTTGGTTAT 
AATTTTATTA CGTCGAGCAC AAAAAATAAT GAAAATGTTG TTTCGATGAG CCTAAATTAT 
CTAAAGGGCA AATTGGATTG GTTGAGGGAT GTGAACAATG ATCAACCGTG TGAAATAGAA 
GATGAGGAGT GGCATTCCAT ACTGGGGAGC GAGGATTTGC TGTCAAAATT GTTACAAAAT 
CCTATGGTGA ACAACCGATT TGAATGGCAA ACAATGTTAT CTAAGGTACT GAAGGGAGAT 
ATTGTGAGGA ATGAAAAAAC GAAGATTGCA AATCAAGGGA AAGGCCCTGG CTTCAATACT 
CAGTTTTCAG ATGACATTTG GATTGAGTTG AAGGCATGGA TGAATGGGAG GACCGTGGAA 
GATCAGAACA AATCTCTGAG GATTTTTAGG GATTCTACTG ACTCCGTATT TCAAGAAATC 
ATGGCCTTTA AACTAGAAGA TAATATGAGC GCTGACGAGG CTGCAGAGAC TATCAAATCA 
CTAGTAGACA AATATTATAG AGTCTTAAAT CTATGGCCTA ACATTAAAAG AATGCATGCT 
GAAAAACCCA TTACTAAAAC AGAAGCATTC AGGAATCGAA TAGATACTTT GAATAGTTGG 
CTTAATTTCA AATTTAACTT TGATACTAAT ATTGCGTACC TGAAAAAATG GATAGTTGGC 
AATAAAGAGC TAGAAAGCAC TACCGAAGTG GATAACACCA CCGTGAATTT GGATGATCCA 
GCCGTTTTCG CCACTAATTG TAAACGCTTT GCGGAGCAAA TTATGAAGGA AAAGGATATT 
GAACTGATAT TTCAAAAAAA AATATTCTTT CCATTAGCAC CATGGATTTT GAAGGCCAAA 
TTTTTCTTCT TGAAATACCA AAAAACTTGG AATGAATTGA ATCTATCTTA TTTGGATCAA 
GATCTGGAAT TTTTATTGAT GTTTCCAATG CGTTTGGTAA AAGATATAAT ACTAATTCGC 
CTATCTTACG CGAAGAAAAT ACAGAATCCA ACCTTGATGA TGATCGATCA AATGATGGAC 
GATTTTAGTA CATATATTAA GTTGGCAGTC CAAATGAAAT TCACTGTTGC TTCTTACTGT 
AATGACTGGT TTTTTAAAGT GAAAATCGAT CCCGAATTTG ATCATACCGT TGTTGAAGGA 
TTGGAATATT TTTTCTCCAT TTTGGAACTG AGAATATTAT ATAGTGGCAA AAACTCATTC 
AAGACTTCTA AAGAACCTGA TCTGTTATTA AAATATTGGG AAATGTTTAG AAACGTCGGC 
TATTATATTG ATGATGCAGG CGAACTGATC GCAGCAGAAT TTACGAAGTT GACACTTAGA 
TTGGTTCACA GATTGCACGC TTACCTTTTA AGGCAGCAAA ACACTCCGCC AAAATTAGAG 
AATGAAGCTG CTGCTGAAAA ATGGCTGGTG CAAATATTCG AAATACTTGG ATCCATGAAA 
AGAAAACTCA ATAGATTCAC CAATATTTTG ACAAAAGCAT TTCAAAATTT TGTCCGCTAC 
AAGATAGAAG ATCACAACTA TCTGCTAAAG CAACTAAAAG AAACAGGCCA CTTTCTTATA 
TACACAGGAG GTTACCTAGA GCAAAATGGT ACTTATTTAA TTGGTAGCCC AGAGCTATTA 
GGCTGTAAAG ATGATGATAT TTTAAGAATC ATTAAGAATT CAGACATCGG TTGTGATTTA 
GTGCCTAAGC TAGAAATTAA TAACAGTCTG ACAATTTATA ATGCTTTGGA TGATAATTGG 
AACTCCAACT CATCACTGGG TTCAGATATC TCGAATGATG GTACCCCATT TTATTATATC 
AAAAACGATT TGACCACCCA GCCTAGATCT TATAACGGTA ATAGAGTCAA TCGTGAACCG 
GATTTTGAAA ACAGCAGGAG CACGGAGGAA GAGTTTTATG AACTGGAGAC AAGATTGAAC 
TCTCTTGGTT ATGTATTGGT ATTGACTCCG CAAGAGCCAT TACTTTGGGA GGGTGAGATG 
TATAATCTAT CCGATAACAA AACAATTAAA CCAGAGGGAT TGAACTTGAA AGTAATTCCT 
AATTCAATAG ATTTGATGTG CCAAGGATCC AGTTATGCCT TAGAATACCA ATGTGACAGG 
TTCCAACAGA TATCTGGTAG CTCAGTTTCT TTCTTGGAAA AAAAATCTTC CTCAGAAACG 
GTTAAAAACA ACTTACAAAG GATAAATAAA GCATATTTCA GATGCACGTA CAGTGTTCTG 
AAGAACTATA CAAAGATTGT GACCACGTTC AAGAAGGTAA GTCCTGTCAA TGATTTATTG 
AATAATATTT TCCTTTTTGG GAGGGATTTT GGTCTGAACT TTTTGAGAAT TAATGTTGCC 
AACAATGAAA AAAGATCCAT TATAATACTT TTAATGATGC GGTTAAGTAT CGGATGGCTG 
AAGTTCTTAG CTGAAGACTG TGATCCGACT GATCAAAGGG TATTTAGGTG GTGTGTCACG 
TCAATGGAGT TCGCGATGCA TATGGTAAGC GGTTGGAACA TACTAGCTCT TGATGAATGC 
CAATTTTCTT CTTTAAAACA AAAAATTTCA GAATGCATGT CATTACTTAT TTCTCATTTT 
GATATAATAG GCGCACGCTC CATAGAAGTT GAGAAAATCA ATCAACAAGC TAGATCAAAT 
CTTGATTTGG AAGATGTGTT TGACGATGAT ATGATGCTAC AAGTGAATTC CGAGTTCCGA 
GTACAAAGTA TAATGGAATT GGAAGAAAGG ATAAAGCGGA ATCCTCATCA AACTGGTAAA 
GTAATTGATG ATAGTGACAA AGGTAACAAG TACCTTGTTT CTTTGGCATC TTCCATATCG 
AACGTTTCTA TGAGATGGCA AAAGAGGAAC TTTATTGGTG GCGGTACTTT TGGAAGGGTA 
TATTCTGCTG TTGATTTGGA TAATGGTGAG ATTTTAGCAG TCAAGGAAAT CAATATTCAA 
GATAGCAAAT CAATGCAAAA AATATTCCCC TTAATCAAGG AGGAAATGAG TGTCTTAGAG 
ATATTGAACC ATCCAAATAT AGTTTCATAT TACGGTGTTG AAGTTCATCG TGATAAAGTT 
AACATCTTTA TGGAATATTG TGAAGGCGGT TCCCTAGCAG CTCTTTTGGA GCATGGTCGT 
ATTGAAGATG AAATGGTCAC TCAAGTCTAC ACTTTACAAT TGCTAGAAGG ACTTGCATAT 
TTGCATGAAT CCGGCATTGT TCACCGTGAT GTTAAACCCG AAAACATCCT ACTAGATTTT 
AATGGTGTTA TTAAGTATGT TGATTTTGGT GCTGCTAAAA AAATTGCTAA TAATGGAACT 
AGATTGGCAA GTATGAACAA AATCGAAAAC GCAGATGGTG AACACGAAGA TGTTACCCAT 
GTTTCTGATT CAAAGGCAGT GAAAAATAAC GAAAATGCTC TATTAGACAT GATGGGAACT 
CCTATGTACA TGGCTCCAGA ATCCATCACT GGATCTACCA CCAAAGGCAA ACTTGGGGCA 
GACGATGTTT GGTCGTTAGG CTGCGTGGTA TTAGAAATGA TCACTGGTAG ACGGCCATGG 
GCTAACTTAG ATAATGAATG GGCTATCATG TACCATGTTG CTGCAGGACA TACCCCACAA 
TTCCCTACTA AGGATGAAGT GTCGTCTGCT GGTATGAAAT TTCTGGAAAG ATGTCTTATT 
CAAAACCCCT CCAAGAGAGC CAGTGCGGTT GAGCTGTTGA TGGATCCTTG GATTGTACAA 
ATTAGAGAAA TAGCCTTTGG CGACGATTCT TCCTCTACAG ATACTGAGGA AAGAGAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The nucleotide sequence of Saccharomyces cerevisiae chromosome XIV and its evolutionary implications.
Nature387(6632 Suppl)93-8(1997 May)
Philippsen P,Kleine K,P?hlmann R,D?sterh?ft A,Hamberg K,Hegemann JH,Obermaier B,Urrestarazu LA,Aert R,Albermann K,Altmann R,Andr? B,Baladron V,Ballesta JP,B?cam AM,Beinhauer J,Boskovic J,Buitrago MJ,Bussereau F,Coster F,Crouzet M,D'Angelo M,Dal Pero F,De Antoni A,Del Rey F,Doignon F,Domdey H,Dubois E,Fiedler T,Fleig U,Floeth M,Fritz C,Gaillardin C,Garcia-Cantalejo JM,Glansdorff NN,Goffeau A,Gueldener U,Herbert C,Heumann K,Heuss-Neitzel D,Hilbert H,Hinni K,Iraqui Houssaini I,Jacquet M,Jimenez A,Jonniaux JL,Karpfinger L,Lanfranchi G,Lepingle A,Levesque H,Lyck R,Maftahi M,Mallet L,Maurer KC,Messenguy F,Mewes HW,M?sti D,Nasr F,Nicaud JM,Niedenthal RK,Pandolfo D,Pi?rard A,Piravandi E,Planta RJ,Pohl TM,Purnelle B,Rebischung C,Remacha M,Revuelta JL,Rinke M,Saiz JE,Sartorello F,Scherens B,Sen-Gupta M,Soler-Mira A,Urbanus JH,Valle G,Van Dyck L,Verhasselt P,Vierendeels F,Vissers S,Voet M,Volckaert G,Wach A,Wambutt R,Wedler H,Zollner A,Hani J

Life with 6000 genes.
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)
Goffeau A,Barrell BG,Bussey H,Davis RW,Dujon B,Feldmann H,Galibert F,Hoheisel JD,Jacq C,Johnston M,Louis EJ,Mewes HW,Murakami Y,Philippsen P,Tettelin H,Oliver SG

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SSK2 cDNA ORF clone, Saccharomyces cerevisiae S288C

Gene

Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

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