GCD10 cDNA ORF clone, Saccharomyces cerevisiae S288C

Gene
Clones
gRNAs

Summary

Gene Symbol	GCD10
Entrez Gene ID	855663
Full Name	tRNA 1-methyladenosine methyltransferase subunit GCD10
Synonyms	TRM6
Gene Type	protein-coding
Organism	Saccharomyces cerevisiae S288C

mRNA and Protein(s)

mRNA	Protein	Name
NM_001182900.3	NP_014337.3	tRNA 1-methyladenosine methyltransferase subunit GCD10

Pathways from BioSystems

Homologies

Schizosaccharomyces pombe (fission yeast)	gcd10	NP_595109.1
Magnaporthe oryzae (rice blast fungus)	MGG_00308	XP_003718782.1
Saccharomyces cerevisiae (baker's yeast)	GCD10	NP_014337.3
Eremothecium gossypii	AGOS_ADL255C	NP_983841.1
Kluyveromyces lactis	KLLA0F03377g	XP_455233.1
Neurospora crassa	NCU09571	XP_959682.1

Gene Ontology
Bibliography

Related articles in PubMed

The nucleotide sequence of Saccharomyces cerevisiae chromosome XIV and its evolutionary implications.
Philippsen P, Kleine K, P?hlmann R, D?sterh?ft A, Hamberg K, Hegemann JH, Obermaier B, Urrestarazu LA, Aert R, Albermann K, Altmann R, Andr? B, Baladron V, Ballesta JP, B?cam AM, Beinhauer J, Boskovic J, Buitrago MJ, Bussereau F, Coster F, Crouzet M, D'Angelo M, Dal Pero F, De Antoni A, Del Rey F, Doignon F, Domdey H, Dubois E, Fiedler T, Fleig U, Floeth M, Fritz C, Gaillardin C, Garcia-Cantalejo JM, Glansdorff NN, Goffeau A, Gueldener U, Herbert C, Heumann K, Heuss-Neitzel D, Hilbert H, Hinni K, Iraqui Houssaini I, Jacquet M, Jimenez A, Jonniaux JL, Karpfinger L, Lanfranchi G, Lepingle A, Levesque H, Lyck R, Maftahi M, Mallet L, Maurer KC, Messenguy F, Mewes HW, M?sti D, Nasr F, Nicaud JM, Niedenthal RK, Pandolfo D, Pi?rard A, Piravandi E, Planta RJ, Pohl TM, Purnelle B, Rebischung C, Remacha M, Revuelta JL, Rinke M, Saiz JE, Sartorello F, Scherens B, Sen-Gupta M, Soler-Mira A, Urbanus JH, Valle G, Van Dyck L, Verhasselt P, Vierendeels F, Vissers S, Voet M, Volckaert G, Wach A, Wambutt R, Wedler H, Zollner A, Hani J
Nature387(6632 Suppl)93-8(1997 May)

Life with 6000 genes.
Goffeau A, Barrell BG, Bussey H, Davis RW, Dujon B, Feldmann H, Galibert F, Hoheisel JD, Jacq C, Johnston M, Louis EJ, Mewes HW, Murakami Y, Philippsen P, Tettelin H, Oliver SG
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

X-ray diffraction analysis of the TRM6-TRM61 complex were performed in this study. The crystals diffracted to 2.80 A resolution and belonged to the trigonal space group P3(1)21 or P3(2)21, with unit-cell parameters a = b = 139.14, c = 101.62 A
Title: Crystallization and preliminary X-ray diffraction crystallographic study of tRNA m(1)A58 methyltransferase from Saccharomyces cerevisiae.

The following GCD10 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the GCD10 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OSi04569	NM_001182900.3 Latest version!	Saccharomyces cerevisiae S288C tRNA 1-methyladenosine methyltransferase subunit GCD10 (GCD10), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

GCD10 ( NM_001182900.3 ) cDNA ORF clone, Saccharomyces cerevisiae S288C -> NP_014337.3 Saccharomyces cerevisiae S288C tRNA 1-methyladenosine methyltransferase subunit GCD10 (GCD10), partial mRNA.

Price & Availability↑

CloneID	OSi04569
Clone ID Related Accession (Same CDS sequence)	NM_001182900.3
Accession Version	NM_001182900.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1437bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-31
Organism	Saccharomyces cerevisiae S288C
Product	tRNA 1-methyladenosine methyltransferase subunit GCD10
Comment	Comment: REVIEWED REFSEQ: This record has been curated by SGD. This record is derived from an annotated genomic sequence (NC_001146). On Jul 30, 2012 this sequence version replaced NM_001182900.2. ##Genome-Annotation-Data-START## Annotation Provider :: SGD Annotation Status :: Full Annotation Annotation Version :: R64-2-1 URL :: http://www.yeastgenome.org/ ##Genome-Annotation-Data-END## COMPLETENESS: incomplete on both ends.

ATGAATGCTT TGACAACCAT AGATTTTAAT CAGCACGTTA TCGTCCGGCT GCCATCAAAA 
AATTACAAGA TAGTGGAACT AAAGCCCAAC ACCTCTGTAT CATTAGGAAA ATTCGGTGCT 
TTCGAGGTTA ATGACATAAT TGGTTATCCT TTCGGGTTGA CTTTCGAGAT ATATTATGAT 
GGCGAAGAGG TTTCATCCGA TGAAAATAGA GATTCGAAAC CAAAGAACAA AATTCCCATC 
GGGAAAGTAA GGCTATTATC GCAGGAGATC AAAGATGTCA ATAATGATAA AGACGATGGT 
CAAAGCGAGC CTCCTTTATC TATTAAGGAA AAATCTGTGT CTCTTGAATT GTCTAGCATT 
GACAGCAGTG CTACGAATCA GAATCTGGTT AACATGGGCA GCAAGGCCCA AGAGTTAACC 
GTTGAAGAAA TTGAAAAAAT GAAACAAGAA TCTTTATCTA GTAAAGAAAT AATCGACAAA 
ATTATCAAAT CTCACAAAAG TTTTCACAAC AAGACCGTAT ATTCTCAAGA GAAATATGTG 
AATAGGAAGA AACAAAAATT CGCCAAGTAC TTCACCGTTG AGTATTTAAG TAGCTCTAAC 
CTTTTACAAT TCTTAATCGA CAAAGGCGAT ATCCAAAGGG TATTGGATAT GTCTCAGGAA 
TCGATGGGGA TGCTGTTAAA TCTTGCGAAC ATCCAATCAG AAGGCAACTA TCTCTGTATG 
GATGAAACGG GTGGGTTATT GGTTTACTTT CTGTTGGAAA GAATGTTTGG CGGTGACAAT 
GAAAGCAAAT CCAAGGGCAA AGTCATAGTT ATTCACGAAA ATGAACATGC CAACTTGGAT 
TTGCTGAAGT TTGCTAATTA TTCTGAGAAA TTTATTAAAG AGCATGTACA CACCATTTCT 
CTATTAGATT TTTTTGAACC GCCTACACTA CAAGAGATTC AGAGCAGATT TACACCATTA 
CCTAAAGAAG AAGCCCGTGC ATTAAAAGGT GGTAAGAAAA ACTCGTACTA CCGAAAACTC 
AGATGGTACA ATACACAATG GCAAATTTTA GAACTAACAG GAGAATTCCT ATATGACGGT 
CTTGTAATGG CTACTACACT ACATTTACCA ACACTAGTGC CAAAGTTGGC CGAAAAAATC 
CATGGTTCAA GGCCGATTGT ATGCTATGGT CAATTCAAAG AAACTTTGTT GGAATTAGCT 
CATACTTTAT ATTCAGACCT CAGGTTTTTA GCACCATCAA TTTTGGAAAC TAGATGTAGG 
CCGTACCAAA GTATAAGGGG TAAGCTACAC CCTCTGATGA CCATGAAAGG TGGTGGTGGA 
TATCTAATGT GGTGTCACAG AGTCATCCCT GCTCCTGAAC CTGTATCGGA AAACGCGACT 
GCAGCTGACT CCAGTGAAAA GCTTGCTGAA CATGGAGCTA AGAAACAAAA AATATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_014337.3
CDS	1..1437
Translation	MNALTTIDFNQHVIVRLPSKNYKIVELKPNTSVSLGKFGAFEVNDIIGYPFGLTFEIYYDGEEVSSDENRDSKPKNKIPIGKVRLLSQEIKDVNNDKDDGQSEPPLSIKEKSVSLELSSIDSSATNQNLVNMGSKAQELTVEEIEKMKQESLSSKEIIDKIIKSHKSFHNKTVYSQEKYVNRKKQKFAKYFTVEYLSSSNLLQFLIDKGDIQRVLDMSQESMGMLLNLANIQSEGNYLCMDETGGLLVYFLLERMFGGDNESKSKGKVIVIHENEHANLDLLKFANYSEKFIKEHVHTISLLDFFEPPTLQEIQSRFTPLPKEEARALKGGKKNSYYRKLRWYNTQWQILELTGEFLYDGLVMATTLHLPTLVPKLAEKIHGSRPIVCYGQFKETLLELAHTLYSDLRFLAPSILETRCRPYQSIRGKLHPLMTMKGGGGYLMWCHRVIPAPEPVSENATAADSSEKLAEHGAKKQKI

Target ORF information:

RefSeq Version	NM_001182900.3
Organism	Saccharomyces cerevisiae S288C
Definition	Saccharomyces cerevisiae S288C tRNA 1-methyladenosine methyltransferase subunit GCD10 (GCD10), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001182900.3

ORF Insert Sequence:

ATGAATGCTT TGACAACCAT AGATTTTAAT CAGCACGTTA TCGTCCGGCT GCCATCAAAA 
AATTACAAGA TAGTGGAACT AAAGCCCAAC ACCTCTGTAT CATTAGGAAA ATTCGGTGCT 
TTCGAGGTTA ATGACATAAT TGGTTATCCT TTCGGGTTGA CTTTCGAGAT ATATTATGAT 
GGCGAAGAGG TTTCATCCGA TGAAAATAGA GATTCGAAAC CAAAGAACAA AATTCCCATC 
GGGAAAGTAA GGCTATTATC GCAGGAGATC AAAGATGTCA ATAATGATAA AGACGATGGT 
CAAAGCGAGC CTCCTTTATC TATTAAGGAA AAATCTGTGT CTCTTGAATT GTCTAGCATT 
GACAGCAGTG CTACGAATCA GAATCTGGTT AACATGGGCA GCAAGGCCCA AGAGTTAACC 
GTTGAAGAAA TTGAAAAAAT GAAACAAGAA TCTTTATCTA GTAAAGAAAT AATCGACAAA 
ATTATCAAAT CTCACAAAAG TTTTCACAAC AAGACCGTAT ATTCTCAAGA GAAATATGTG 
AATAGGAAGA AACAAAAATT CGCCAAGTAC TTCACCGTTG AGTATTTAAG TAGCTCTAAC 
CTTTTACAAT TCTTAATCGA CAAAGGCGAT ATCCAAAGGG TATTGGATAT GTCTCAGGAA 
TCGATGGGGA TGCTGTTAAA TCTTGCGAAC ATCCAATCAG AAGGCAACTA TCTCTGTATG 
GATGAAACGG GTGGGTTATT GGTTTACTTT CTGTTGGAAA GAATGTTTGG CGGTGACAAT 
GAAAGCAAAT CCAAGGGCAA AGTCATAGTT ATTCACGAAA ATGAACATGC CAACTTGGAT 
TTGCTGAAGT TTGCTAATTA TTCTGAGAAA TTTATTAAAG AGCATGTACA CACCATTTCT 
CTATTAGATT TTTTTGAACC GCCTACACTA CAAGAGATTC AGAGCAGATT TACACCATTA 
CCTAAAGAAG AAGCCCGTGC ATTAAAAGGT GGTAAGAAAA ACTCGTACTA CCGAAAACTC 
AGATGGTACA ATACACAATG GCAAATTTTA GAACTAACAG GAGAATTCCT ATATGACGGT 
CTTGTAATGG CTACTACACT ACATTTACCA ACACTAGTGC CAAAGTTGGC CGAAAAAATC 
CATGGTTCAA GGCCGATTGT ATGCTATGGT CAATTCAAAG AAACTTTGTT GGAATTAGCT 
CATACTTTAT ATTCAGACCT CAGGTTTTTA GCACCATCAA TTTTGGAAAC TAGATGTAGG 
CCGTACCAAA GTATAAGGGG TAAGCTACAC CCTCTGATGA CCATGAAAGG TGGTGGTGGA 
TATCTAATGT GGTGTCACAG AGTCATCCCT GCTCCTGAAC CTGTATCGGA AAACGCGACT 
GCAGCTGACT CCAGTGAAAA GCTTGCTGAA CATGGAGCTA AGAAACAAAA AATATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The nucleotide sequence of Saccharomyces cerevisiae chromosome XIV and its evolutionary implications.
Nature387(6632 Suppl)93-8(1997 May)
Philippsen P,Kleine K,P?hlmann R,D?sterh?ft A,Hamberg K,Hegemann JH,Obermaier B,Urrestarazu LA,Aert R,Albermann K,Altmann R,Andr? B,Baladron V,Ballesta JP,B?cam AM,Beinhauer J,Boskovic J,Buitrago MJ,Bussereau F,Coster F,Crouzet M,D'Angelo M,Dal Pero F,De Antoni A,Del Rey F,Doignon F,Domdey H,Dubois E,Fiedler T,Fleig U,Floeth M,Fritz C,Gaillardin C,Garcia-Cantalejo JM,Glansdorff NN,Goffeau A,Gueldener U,Herbert C,Heumann K,Heuss-Neitzel D,Hilbert H,Hinni K,Iraqui Houssaini I,Jacquet M,Jimenez A,Jonniaux JL,Karpfinger L,Lanfranchi G,Lepingle A,Levesque H,Lyck R,Maftahi M,Mallet L,Maurer KC,Messenguy F,Mewes HW,M?sti D,Nasr F,Nicaud JM,Niedenthal RK,Pandolfo D,Pi?rard A,Piravandi E,Planta RJ,Pohl TM,Purnelle B,Rebischung C,Remacha M,Revuelta JL,Rinke M,Saiz JE,Sartorello F,Scherens B,Sen-Gupta M,Soler-Mira A,Urbanus JH,Valle G,Van Dyck L,Verhasselt P,Vierendeels F,Vissers S,Voet M,Volckaert G,Wach A,Wambutt R,Wedler H,Zollner A,Hani J

Life with 6000 genes.
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)
Goffeau A,Barrell BG,Bussey H,Davis RW,Dujon B,Feldmann H,Galibert F,Hoheisel JD,Jacq C,Johnston M,Louis EJ,Mewes HW,Murakami Y,Philippsen P,Tettelin H,Oliver SG

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GCD10 cDNA ORF clone, Saccharomyces cerevisiae S288C

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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