POP1 cDNA ORF clone, Saccharomyces cerevisiae S288C

Gene
Clones
gRNAs

Summary

Gene Symbol	POP1
Entrez Gene ID	855500
Full Name	ribonuclease P/MRP protein subunit POP1
Gene Type	protein-coding
Organism	Saccharomyces cerevisiae S288C

mRNA and Protein(s)

mRNA	Protein	Name
NM_001183059.1	NP_014178.1	ribonuclease P/MRP protein subunit POP1

Pathways from BioSystems

Homologies

Saccharomyces cerevisiae (baker's yeast)	POP1	NP_014178.1
Eremothecium gossypii	AGOS_ABL187C	NP_982760.1
Kluyveromyces lactis	KLLA0D19448g	XP_453925.1

Gene Ontology
Bibliography

Related articles in PubMed

The nucleotide sequence of Saccharomyces cerevisiae chromosome XIV and its evolutionary implications.
Philippsen P, Kleine K, P?hlmann R, D?sterh?ft A, Hamberg K, Hegemann JH, Obermaier B, Urrestarazu LA, Aert R, Albermann K, Altmann R, Andr? B, Baladron V, Ballesta JP, B?cam AM, Beinhauer J, Boskovic J, Buitrago MJ, Bussereau F, Coster F, Crouzet M, D'Angelo M, Dal Pero F, De Antoni A, Del Rey F, Doignon F, Domdey H, Dubois E, Fiedler T, Fleig U, Floeth M, Fritz C, Gaillardin C, Garcia-Cantalejo JM, Glansdorff NN, Goffeau A, Gueldener U, Herbert C, Heumann K, Heuss-Neitzel D, Hilbert H, Hinni K, Iraqui Houssaini I, Jacquet M, Jimenez A, Jonniaux JL, Karpfinger L, Lanfranchi G, Lepingle A, Levesque H, Lyck R, Maftahi M, Mallet L, Maurer KC, Messenguy F, Mewes HW, M?sti D, Nasr F, Nicaud JM, Niedenthal RK, Pandolfo D, Pi?rard A, Piravandi E, Planta RJ, Pohl TM, Purnelle B, Rebischung C, Remacha M, Revuelta JL, Rinke M, Saiz JE, Sartorello F, Scherens B, Sen-Gupta M, Soler-Mira A, Urbanus JH, Valle G, Van Dyck L, Verhasselt P, Vierendeels F, Vissers S, Voet M, Volckaert G, Wach A, Wambutt R, Wedler H, Zollner A, Hani J
Nature387(6632 Suppl)93-8(1997 May)

Life with 6000 genes.
Goffeau A, Barrell BG, Bussey H, Davis RW, Dujon B, Feldmann H, Galibert F, Hoheisel JD, Jacq C, Johnston M, Louis EJ, Mewes HW, Murakami Y, Philippsen P, Tettelin H, Oliver SG
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Study reports that the Pop1, Pop6, and Pop7 proteins, known components of RNase P and RNase MRP, bind to yeast telomerase RNA and are essential constituents of the telomerase holoenzyme.
Title: Active Yeast Telomerase Shares Subunits with Ribonucleoproteins RNase P and RNase MRP.

Results suggest that Pop1 plays the role of a scaffold for the stabilization of the global architecture of eukaryotic RNase P RNA, substituting for the network of RNA-RNA tertiary interactions that maintain the global RNA structure in bacterial RNase P.
Title: Footprinting analysis of interactions between the largest eukaryotic RNase P/MRP protein Pop1 and RNase P/MRP RNA components.

This study shows that the Pop1p subunit plays multiple roles in the assembly and function of of RNases P and MRP, and that the functions can be differentiated through the mutations in conserved residues.
Title: Functional characterization of the conserved amino acids in Pop1p, the largest common protein subunit of yeast RNases P and MRP.

The following POP1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the POP1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OSi04425	NM_001183059.1 Latest version!	Saccharomyces cerevisiae S288C ribonuclease P/MRP protein subunit POP1 (POP1), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

POP1 ( NM_001183059.1 ) cDNA ORF clone, Saccharomyces cerevisiae S288C -> NP_014178.1 Saccharomyces cerevisiae S288C ribonuclease P/MRP protein subunit POP1 (POP1), partial mRNA.

Price & Availability↑

CloneID	OSi04425
Clone ID Related Accession (Same CDS sequence)	NM_001183059.1
Accession Version	NM_001183059.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2628bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-31
Organism	Saccharomyces cerevisiae S288C
Product	ribonuclease P/MRP protein subunit POP1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by SGD. This record is derived from an annotated genomic sequence (NC_001146). ##Genome-Annotation-Data-START## Annotation Provider :: SGD Annotation Status :: Full Annotation Annotation Version :: R64-2-1 URL :: http://www.yeastgenome.org/ ##Genome-Annotation-Data-END## COMPLETENESS: incomplete on both ends.

ATGAGCGGGA GTTTGTCTAG AGGAAATGGT GGAAAGAAAG TTCTTAATAA GAATCAATTA 
CTGAAGCGAA ATCGCATAAG AAATGCTAGA TCAATTAGAG CAGAGGCCGT GGCCGCTTCA 
TCAACAAAAA CTGGGACACC GAGTGATCTG AGTGAATCGG GAAGTAAGCT AAACGTGGAC 
CAATTCATTA GTTCTCGACA ATTCGAGGTT AAGCAGTTGC AATTGGCCAT GCATAATTCC 
AAAGCAGCCA GCTCAACGAG AATATTTCAG GCATTGCCTC GAAAATTGCG TCGTAGAACG 
GCTTCGCATA ATGTTCGTAG AATTCCAAAA AGAATGCGCA ATAGAGCTTT GAGGGAAATG 
CGTAAAAGTG ATCAGCAGGA TGTGTTGAAG GGGTCTTCAG CCTCTTCCAG AAAAGCTCAT 
GGCCTAAATG CAAAGCAACT TTATAAAGCT AGAATGTCCA TAAAGCTGCT ACGATTGGCT 
AGCAAATCTA CTTCTATGAA ACTCAGCATG CCACCAGAGG TTACATCATC TAACTGCCAC 
GTAAGGCAGA AAATCAAGAC ATTAAAGCGA ATGATTAAAG AATCATCAAC AGCAAATCCA 
AATATAAAGT TACTTAATAA TCGTATGGGG AGCTATGATT GCACTGGTGT CAATGAATTA 
GCACCTATTC CCAAGGGCAG GGTCAAATAT ACGAAGAGAC AGAAGCACTT TGCTTGGCTA 
CCCACCCACA TTTGGAATGC TAAAAGGTCG CACATGATGA AAAGATGGGG ATATCAAATG 
GTATGGGCAC CTACTCAAAA ATGCTTTAAA TTGACGCATA GGTTGGGCGG TGATACCTGC 
AGCTCCGATG GGGCCTTATG CATGGATTCA AGTTATATTG GTACAATAAT TGTAAAAGAC 
AAAAGCAATG ACAGTGAGGG GGACTTTTTA AAATCCATTA TAGGAAAGTT AACAGCTGAA 
AGAGCAAACT TAAGGAAATA TCGCGAAGGC CAGGTTTTAT TCCAAGGGTT AATTTATAGT 
TTCAATGAAG AAAACGGTGA GGATTCTACT AAGCCACTGG GTCCATGCGA CGTATTTTGG 
GTACAAAAGG ATACTGCAAT TATAAGGTTA CACCCTTCAA TTTATACTCA GGTTTTTAAC 
ATTCTTTTGC AACATAAGGA AAAACTAACA GTACAAGATT GTCGATATTC ATTAGCAAGC 
GTGACATTGA AGGGAGCTAA AGCTCTAGAG TCATTAGCAT CGTGTTTAAG GAGCACAGAG 
TACTCTAAAT CGTTTGAACA ATTTAAAATG GTTTCAATGA TTACCGATCA TAATGCCCTA 
CCACAGAGGT GTACGTTTGC ATTTGAAGCC ATAGATCCGC GTCATCTAGC TGCTCCAAAG 
AAATTAAATG ATTCACAGAG GAAGACTGTT AATTCTGACG ACATTTTATC ACTGCATGAA 
AACTATCCTC AAGACGAAAT AAACGCCGTT TTTAACGAAT TATGTGACCC TGAATCACGT 
ACTCAATCAT ACAACAACCA AAACACATTA AAAGAAATTT CTGCGAGACG TTACAAATTG 
CTAACTGCCA CACCGAACAG CATCAATAAA ACTACAGTCC CGTTCAAAGA AAGTGACGAT 
CCTTCTATTC CATTAGTCAT AATAAGAAGG CTCAAGACCA GGGATTGGAT TGTTGTGTTA 
CCATGGTTCT GGCTGCTACC GTTATGGCAT CTACTGAACC GGATACCAAG GATGTATCAC 
ATTGGCCTCA GGCAATTCCA GCAAATTCAG TACGAGAATA AGCAATTATA TTTTCCGGAT 
GATTATCCAT TCACTCAATT GGGTTATATA GAAAACTCAT TTTATAAAAA GGAGGCCTCC 
AAGACGAAGT GGGATAGGAA GCCAATGGGA AAAAGGATTA ATTTCGAGAA GATCAAAGAC 
ATCCATAACA CTAAATTACC GGCATATAGC GGAGAAATCG GCGATTTTTT CAGTTCAGAC 
TGGAGATTTT TACAAATACT GAGAAATGGA ATTGATTATT TACAGCGGAA TGATAAAACC 
TTAGAACTGA TGGACAGTAA GAAGACCGGG CAATTTAACG CTCAGGGTGT AAGAGACATC 
AACTGTGTAA ATGATGTTTT GGAGTTTTGC AAAGATTATG AAGCCAAGAC CAAGGCTATG 
TCGTTGTCGA TTGAAGAGAA CATACCAGTT GCATTGTGTA AAAATCGGAA ATGTCAATTT 
CGCACTCCTG ATAGCATTAG CGTCAATAGT TCTTCATTCT CCCTTACATT CTTCCCACGT 
TGTATCATTG CTGTATCTTG CACACTACTC GAACGAGGTC ACCCAAAGGA CAATGCAAGA 
ATATATCAAG TTCCAGAAAA GGACTTAGAG CATTGGCTAC AATTGGCAAA AGGGGTTTAC 
CGCCCAAACG GAAGAAAAGA TCATGATTTG AAAATTCCAC TTCCAGAGGT ACATGATTTG 
ATTGGTTTTA TTACCAGCGG CACCTACCAC TTAAATTGTG GAAATGGTAT GGGAATCGGA 
TTTATAGATC ATCATGCGGC CATCCGGCAA CCAACTAGGT ATGTACTGAT CAGAAATGTA 
GGTACCAACA CTTATAGGTT GGGGGAGTGG TCGAAAATAT CCGTATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_014178.1
CDS	1..2628
Translation	MSGSLSRGNGGKKVLNKNQLLKRNRIRNARSIRAEAVAASSTKTGTPSDLSESGSKLNVDQFISSRQFEVKQLQLAMHNSKAASSTRIFQALPRKLRRRTASHNVRRIPKRMRNRALREMRKSDQQDVLKGSSASSRKAHGLNAKQLYKARMSIKLLRLASKSTSMKLSMPPEVTSSNCHVRQKIKTLKRMIKESSTANPNIKLLNNRMGSYDCTGVNELAPIPKGRVKYTKRQKHFAWLPTHIWNAKRSHMMKRWGYQMVWAPTQKCFKLTHRLGGDTCSSDGALCMDSSYIGTIIVKDKSNDSEGDFLKSIIGKLTAERANLRKYREGQVLFQGLIYSFNEENGEDSTKPLGPCDVFWVQKDTAIIRLHPSIYTQVFNILLQHKEKLTVQDCRYSLASVTLKGAKALESLASCLRSTEYSKSFEQFKMVSMITDHNALPQRCTFAFEAIDPRHLAAPKKLNDSQRKTVNSDDILSLHENYPQDEINAVFNELCDPESRTQSYNNQNTLKEISARRYKLLTATPNSINKTTVPFKESDDPSIPLVIIRRLKTRDWIVVLPWFWLLPLWHLLNRIPRMYHIGLRQFQQIQYENKQLYFPDDYPFTQLGYIENSFYKKEASKTKWDRKPMGKRINFEKIKDIHNTKLPAYSGEIGDFFSSDWRFLQILRNGIDYLQRNDKTLELMDSKKTGQFNAQGVRDINCVNDVLEFCKDYEAKTKAMSLSIEENIPVALCKNRKCQFRTPDSISVNSSSFSLTFFPRCIIAVSCTLLERGHPKDNARIYQVPEKDLEHWLQLAKGVYRPNGRKDHDLKIPLPEVHDLIGFITSGTYHLNCGNGMGIGFIDHHAAIRQPTRYVLIRNVGTNTYRLGEWSKISV

Target ORF information:

RefSeq Version	NM_001183059.1
Organism	Saccharomyces cerevisiae S288C
Definition	Saccharomyces cerevisiae S288C ribonuclease P/MRP protein subunit POP1 (POP1), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001183059.1

ORF Insert Sequence:

ATGAGCGGGA GTTTGTCTAG AGGAAATGGT GGAAAGAAAG TTCTTAATAA GAATCAATTA 
CTGAAGCGAA ATCGCATAAG AAATGCTAGA TCAATTAGAG CAGAGGCCGT GGCCGCTTCA 
TCAACAAAAA CTGGGACACC GAGTGATCTG AGTGAATCGG GAAGTAAGCT AAACGTGGAC 
CAATTCATTA GTTCTCGACA ATTCGAGGTT AAGCAGTTGC AATTGGCCAT GCATAATTCC 
AAAGCAGCCA GCTCAACGAG AATATTTCAG GCATTGCCTC GAAAATTGCG TCGTAGAACG 
GCTTCGCATA ATGTTCGTAG AATTCCAAAA AGAATGCGCA ATAGAGCTTT GAGGGAAATG 
CGTAAAAGTG ATCAGCAGGA TGTGTTGAAG GGGTCTTCAG CCTCTTCCAG AAAAGCTCAT 
GGCCTAAATG CAAAGCAACT TTATAAAGCT AGAATGTCCA TAAAGCTGCT ACGATTGGCT 
AGCAAATCTA CTTCTATGAA ACTCAGCATG CCACCAGAGG TTACATCATC TAACTGCCAC 
GTAAGGCAGA AAATCAAGAC ATTAAAGCGA ATGATTAAAG AATCATCAAC AGCAAATCCA 
AATATAAAGT TACTTAATAA TCGTATGGGG AGCTATGATT GCACTGGTGT CAATGAATTA 
GCACCTATTC CCAAGGGCAG GGTCAAATAT ACGAAGAGAC AGAAGCACTT TGCTTGGCTA 
CCCACCCACA TTTGGAATGC TAAAAGGTCG CACATGATGA AAAGATGGGG ATATCAAATG 
GTATGGGCAC CTACTCAAAA ATGCTTTAAA TTGACGCATA GGTTGGGCGG TGATACCTGC 
AGCTCCGATG GGGCCTTATG CATGGATTCA AGTTATATTG GTACAATAAT TGTAAAAGAC 
AAAAGCAATG ACAGTGAGGG GGACTTTTTA AAATCCATTA TAGGAAAGTT AACAGCTGAA 
AGAGCAAACT TAAGGAAATA TCGCGAAGGC CAGGTTTTAT TCCAAGGGTT AATTTATAGT 
TTCAATGAAG AAAACGGTGA GGATTCTACT AAGCCACTGG GTCCATGCGA CGTATTTTGG 
GTACAAAAGG ATACTGCAAT TATAAGGTTA CACCCTTCAA TTTATACTCA GGTTTTTAAC 
ATTCTTTTGC AACATAAGGA AAAACTAACA GTACAAGATT GTCGATATTC ATTAGCAAGC 
GTGACATTGA AGGGAGCTAA AGCTCTAGAG TCATTAGCAT CGTGTTTAAG GAGCACAGAG 
TACTCTAAAT CGTTTGAACA ATTTAAAATG GTTTCAATGA TTACCGATCA TAATGCCCTA 
CCACAGAGGT GTACGTTTGC ATTTGAAGCC ATAGATCCGC GTCATCTAGC TGCTCCAAAG 
AAATTAAATG ATTCACAGAG GAAGACTGTT AATTCTGACG ACATTTTATC ACTGCATGAA 
AACTATCCTC AAGACGAAAT AAACGCCGTT TTTAACGAAT TATGTGACCC TGAATCACGT 
ACTCAATCAT ACAACAACCA AAACACATTA AAAGAAATTT CTGCGAGACG TTACAAATTG 
CTAACTGCCA CACCGAACAG CATCAATAAA ACTACAGTCC CGTTCAAAGA AAGTGACGAT 
CCTTCTATTC CATTAGTCAT AATAAGAAGG CTCAAGACCA GGGATTGGAT TGTTGTGTTA 
CCATGGTTCT GGCTGCTACC GTTATGGCAT CTACTGAACC GGATACCAAG GATGTATCAC 
ATTGGCCTCA GGCAATTCCA GCAAATTCAG TACGAGAATA AGCAATTATA TTTTCCGGAT 
GATTATCCAT TCACTCAATT GGGTTATATA GAAAACTCAT TTTATAAAAA GGAGGCCTCC 
AAGACGAAGT GGGATAGGAA GCCAATGGGA AAAAGGATTA ATTTCGAGAA GATCAAAGAC 
ATCCATAACA CTAAATTACC GGCATATAGC GGAGAAATCG GCGATTTTTT CAGTTCAGAC 
TGGAGATTTT TACAAATACT GAGAAATGGA ATTGATTATT TACAGCGGAA TGATAAAACC 
TTAGAACTGA TGGACAGTAA GAAGACCGGG CAATTTAACG CTCAGGGTGT AAGAGACATC 
AACTGTGTAA ATGATGTTTT GGAGTTTTGC AAAGATTATG AAGCCAAGAC CAAGGCTATG 
TCGTTGTCGA TTGAAGAGAA CATACCAGTT GCATTGTGTA AAAATCGGAA ATGTCAATTT 
CGCACTCCTG ATAGCATTAG CGTCAATAGT TCTTCATTCT CCCTTACATT CTTCCCACGT 
TGTATCATTG CTGTATCTTG CACACTACTC GAACGAGGTC ACCCAAAGGA CAATGCAAGA 
ATATATCAAG TTCCAGAAAA GGACTTAGAG CATTGGCTAC AATTGGCAAA AGGGGTTTAC 
CGCCCAAACG GAAGAAAAGA TCATGATTTG AAAATTCCAC TTCCAGAGGT ACATGATTTG 
ATTGGTTTTA TTACCAGCGG CACCTACCAC TTAAATTGTG GAAATGGTAT GGGAATCGGA 
TTTATAGATC ATCATGCGGC CATCCGGCAA CCAACTAGGT ATGTACTGAT CAGAAATGTA 
GGTACCAACA CTTATAGGTT GGGGGAGTGG TCGAAAATAT CCGTATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The nucleotide sequence of Saccharomyces cerevisiae chromosome XIV and its evolutionary implications.
Nature387(6632 Suppl)93-8(1997 May)
Philippsen P,Kleine K,P?hlmann R,D?sterh?ft A,Hamberg K,Hegemann JH,Obermaier B,Urrestarazu LA,Aert R,Albermann K,Altmann R,Andr? B,Baladron V,Ballesta JP,B?cam AM,Beinhauer J,Boskovic J,Buitrago MJ,Bussereau F,Coster F,Crouzet M,D'Angelo M,Dal Pero F,De Antoni A,Del Rey F,Doignon F,Domdey H,Dubois E,Fiedler T,Fleig U,Floeth M,Fritz C,Gaillardin C,Garcia-Cantalejo JM,Glansdorff NN,Goffeau A,Gueldener U,Herbert C,Heumann K,Heuss-Neitzel D,Hilbert H,Hinni K,Iraqui Houssaini I,Jacquet M,Jimenez A,Jonniaux JL,Karpfinger L,Lanfranchi G,Lepingle A,Levesque H,Lyck R,Maftahi M,Mallet L,Maurer KC,Messenguy F,Mewes HW,M?sti D,Nasr F,Nicaud JM,Niedenthal RK,Pandolfo D,Pi?rard A,Piravandi E,Planta RJ,Pohl TM,Purnelle B,Rebischung C,Remacha M,Revuelta JL,Rinke M,Saiz JE,Sartorello F,Scherens B,Sen-Gupta M,Soler-Mira A,Urbanus JH,Valle G,Van Dyck L,Verhasselt P,Vierendeels F,Vissers S,Voet M,Volckaert G,Wach A,Wambutt R,Wedler H,Zollner A,Hani J

Life with 6000 genes.
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)
Goffeau A,Barrell BG,Bussey H,Davis RW,Dujon B,Feldmann H,Galibert F,Hoheisel JD,Jacq C,Johnston M,Louis EJ,Mewes HW,Murakami Y,Philippsen P,Tettelin H,Oliver SG

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POP1 cDNA ORF clone, Saccharomyces cerevisiae S288C

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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