CWC25 cDNA ORF clone, Saccharomyces cerevisiae S288C

The nucleotide sequence of Saccharomyces cerevisiae chromosome XIV and its evolutionary implications.
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Nature387(6632 Suppl)93-8(1997 May)

Life with 6000 genes.
Goffeau A, Barrell BG, Bussey H, Davis RW, Dujon B, Feldmann H, Galibert F, Hoheisel JD, Jacq C, Johnston M, Louis EJ, Mewes HW, Murakami Y, Philippsen P, Tettelin H, Oliver SG
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)

In the process of pre-mRNA splicing, Cwc25 binds tightly to the spliceosome after the reaction and is then removed from the spliceosome, which normally requires Prp16 and ATP hydrolysis, to allow the occurrence of the second reaction.
Title: A central role of Cwc25 in spliceosome dynamics during the catalytic phase of pre-mRNA splicing.

Upon step 1 catalysis, Cwc25 contacts with the branch-site region, and enhanced crosslinks of Prp8 and Prp45 with nucleotides surrounding the branch-site were observed.
Title: Dynamic Contacts of U2, RES, Cwc25, Prp8 and Prp45 Proteins with the Pre-mRNA Branch-Site and 3' Splice Site during Catalytic Activation and Step 1 Catalysis in Yeast Spliceosomes.

Cwc25 functions in the final step of the first catalytic reaction after the action of Prp2.
Title: Cwc25 is a novel splicing factor required after Prp2 and Yju2 to facilitate the first catalytic reaction.