PEP5 cDNA ORF clone, Saccharomyces cerevisiae S288C

Gene
Clones
gRNAs

Summary

Gene Symbol	PEP5
Entrez Gene ID	855271
Full Name	tethering complex subunit PEP5
Synonyms	END1,VAM1,VPL9,VPS11,VPT11
Gene Type	protein-coding
Organism	Saccharomyces cerevisiae S288C

mRNA and Protein(s)

mRNA	Protein	Name
NM_001182738.1	NP_013958.1	tethering complex subunit PEP5

Pathways from BioSystems

Homologies

Saccharomyces cerevisiae (baker's yeast)	PEP5	NP_013958.1
Kluyveromyces lactis	KLLA0B13090g	XP_452114.1
Eremothecium gossypii	AGOS_ABL012C	NP_982935.2

Gene Ontology
Bibliography

Related articles in PubMed

The nucleotide sequence of Saccharomyces cerevisiae chromosome XIII.
Bowman S, Churcher C, Badcock K, Brown D, Chillingworth T, Connor R, Dedman K, Devlin K, Gentles S, Hamlin N, Hunt S, Jagels K, Lye G, Moule S, Odell C, Pearson D, Rajandream M, Rice P, Skelton J, Walsh S, Whitehead S, Barrell B
Nature387(6632 Suppl)90-3(1997 May)

Life with 6000 genes.
Goffeau A, Barrell BG, Bussey H, Davis RW, Dujon B, Feldmann H, Galibert F, Hoheisel JD, Jacq C, Johnston M, Louis EJ, Mewes HW, Murakami Y, Philippsen P, Tettelin H, Oliver SG
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The results highlight the importance of posttranslational histone regulatory mechanisms that employ multiple E3 ubiquitin ligases such as Pep5 to ensure excess histone degradation and thus contribute to the maintenance of genomic stability.
Title: Novel E3 ubiquitin ligases that regulate histone protein levels in the budding yeast Saccharomyces cerevisiae.

The C-terminal domain of Vps11 is a key integrator of CORVET (class C core vacuole/endosome tethering complex) assembly, Rab signaling, and endosomal and lysosomal traffic.
Title: Subunit organization and Rab interactions of Vps-C protein complexes that control endolysosomal membrane traffic.

The following PEP5 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the PEP5 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OSi04226	NM_001182738.1 Latest version!	Saccharomyces cerevisiae S288C tethering complex subunit PEP5 (PEP5), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

PEP5 ( NM_001182738.1 ) cDNA ORF clone, Saccharomyces cerevisiae S288C -> NP_013958.1 Saccharomyces cerevisiae S288C tethering complex subunit PEP5 (PEP5), partial mRNA.

Price & Availability↑

CloneID	OSi04226
Clone ID Related Accession (Same CDS sequence)	NM_001182738.1
Accession Version	NM_001182738.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3090bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-31
Organism	Saccharomyces cerevisiae S288C
Product	tethering complex subunit PEP5
Comment	Comment: REVIEWED REFSEQ: This record has been curated by SGD. This record is derived from an annotated genomic sequence (NC_001145). ##Genome-Annotation-Data-START## Annotation Provider :: SGD Annotation Status :: Full Annotation Annotation Version :: R64-2-1 URL :: http://www.yeastgenome.org/ ##Genome-Annotation-Data-END## COMPLETENESS: incomplete on both ends.

ATGTCCCTGA GCTCCTGGAG GCAATTCCAG CTTTTCGAGA ATATTCCCAT AAGAGATCCT 
AATTTTGGAG GAGATTCCCT ATTGTACTCG GACCCAACAC TTTGCGCAGC TACAATAGTA 
GATCCTCAAA CTCTGATCAT AGCTGTAAAT TCAAATATTA TAAAAGTTGT CAAATTAAAT 
CAATCGCAAG TAATACATGA ATTTCAATCT TTTCCTCATG ATTTTCAAAT CACTTTCCTG 
AAAGTCATCA ACGGGGAATT TCTCGTAGCG TTAGCTGAAT CCATTGGCAA ACCTTCCCTA 
ATAAGAGTAT ATAAACTAGA AAAACTGCCA AATAGAGAAC AACTATACCA TTCACAAGTC 
GAACTGAAAA ACGGTAATAA TACATACCCT ATTTCAGTTG TCTCCATATC AAATGACCTT 
TCCTGTATTG TGGTTGGATT CATTAATGGG AAAATCATCC TTATTAGAGG TGACATTTCA 
AGAGATAGAG GATCTCAACA AAGGATTATA TATGAAGATC CAAGTAAAGA GCCAATAACA 
GCTTTATTCC TCAATAACGA CGCAACGGCT TGTTTTGCGG CTACAACTTC AAGAATCCTT 
TTATTTAATA CGACTGGTAG AAATAGAGGG CGCCCAAGTT TGGTTTTAAA TTCAAAAAAT 
GGCCTGGATC TAAATTGTGG ATCTTTTAAT CCGGCAACAA ACGAATTTAT ATGTTGCTTA 
AGCAACTTTA TCGAATTTTT CAGCTCTAGT GGAAAAAAGC ATCAATTCGC ATTTGATCTA 
TCGCTGAGAA AGAGGATATT TTGTGTAGAT AAAGATCACA TTTTGATTGT GACTGAGGAA 
ACAGGTGTAC CAACTACATC CATAAGTGTT AATGAACTGT CGCCCACAAT AATTAACCGA 
ATATTCATCA TTGATGCTAA AAATAAAATC ATTTCTTTGA ATTTTGTTGT TTCTAGTGCA 
ATTATTGATA TTTTTTCAAC CTCTCAGAGC GGTAAAAACA TCACTTATCT GCTAACTTCT 
GAAGGAGTAA TGCATAGGAT AACTCCAAAA TCTTTAGAAA ATCAAATTAA CATCATCATC 
CAAAAGGAAC TGTACCCTTT TGCTTTACAA TTAGCGAAAC AGCATTCATT ATCACCGCTA 
GATGTTCAGG AAATTCATAA AAAATATGGT GATTATCTCT TTAAAAAGGG ACTCAGGAAG 
GAAGCAACAG ACCAGTACAT ACAATGTCTG GACGTTGTGG AAACTAGTGA AATCATTTCA 
AAATTTGGTG TCAAGGAGGT TCCTGACCCA GAGAGTATGA GGAACTTGGC TGACTATCTG 
TGGTCTTTGA TCAAGAATTC TATTTCTCAA CGCGATCATG TTACCCTTTT GTTAATTGTT 
TTAATCAAAT TGAAGGATGT TGAAGGAATT GATACTTTTA TCCAGCATTT CGATAGAAAA 
GGCATTTGGA ATGAAGGTGT TGTGATGGAC GATATGGATG ATGTGACGTT TTTTTATTCA 
GATAACGATT TTTTTGATTT AGATTTAATC TTAGAGCTTA TGAAAGAGTC AGATTTTAAG 
CGTCTCTCAT ACAGACTTGC AAAGAAGTAT TCAAAGGATT CATTAATTAT TGTGGATATT 
TTATTGAATC TATTGCATAA CCCTGTAAAA GCCATAAAAT ACATAAAAAG TTTACCAATT 
GATGAAACCC TAAGATGCCT TGTGACGTAC TCGAAAAAAT TACTGGAGGA GTCACCGAAT 
GAAACTAATG CTTTACTGAT AGAAGTATTT ACAGGGAAGT TCAAACCATC CACTTTTGAA 
GTGGATCTGG ACCGGAGAGA TACGACGGGC GATTTTTCTG AAAATATTAG AACAGTTTTT 
TACAGTTACA AAACATTCTT CAACTACATG AACTCAAATG GTACATCAGA CGCAATGAGC 
GAGTCTTCAG AGGCATCCCA CGAACATGAA GAGCCTACTT ATCATCCGCC GAAGCCATCC 
ATTGTTTTCA GTTCATTCGT TACAAAACCT TTTGAATTTG TTGTTTTCTT AGAAGCCTGT 
TTGGCATGTT ACCAGCAGTA TGAGGGTTTT GACGAAGATA GGCAAGTAAT TTTGACTACT 
TTATATGACT TATATTTGAA TTTGGCGCAA AATGATGTAC CCGAGCGTAT AGACGACTGG 
CGTTCAAGAG CAACTGGCGT ATTGCGTGAG AGCAATAAAT TGGTGTATTC TGCCGCAAGT 
AATAATACTA GCAAAAGGGT GGATAACTCA ATAATGCTGT TAATTTCCCA TATGGATCAA 
AGTAGTGCTT CAGCAAAAGA TAAGACGAAA ATTGACATAG CTTCATTTGC CAATGATAAC 
CCCGAGATGG ATTTGCTGAG TACATTTAGG GCTATGACGT TAAATGAAGA ACCAAGTACT 
TGTCTCAAAT TCCTAGAAAA ATATGGCACA GAGGAACCCA AGCTCTTACA AGTAGCATTG 
AGTTACTTTG TTTCTAATAA ACTAATCTTC AAGGAGATGG GCGGTAATGA AGTACTGAAA 
GAAAAAGTAT TGAGGCCAAT TATAGAGGGG GAAAGAATGC CACTGTTGGA TATAATTAAA 
GCGCTATCCC GTACAAATGT AGCCCACTTT GGGCTGATAC AAGACATCAT TATTGATCAT 
GTCAAAACCG AAGATACAGA AATCAAAAGG AACGAAAAAC TAATTGAATC TTACGATAAA 
GAGTTAAAGG AGAAAAACAA GAAGTTGAAG AACACCATTA ATTCAGATCA ACCTCTCCAC 
GTACCCCTGA AGAATCAAAC GTGTTTCATG TGTAGACTGA CATTGGATAT TCCTGTAGTT 
TTTTTTAAAT GTGGTCACAT TTACCACCAA CATTGTCTAA ATGAGGAAGA AGATACTCTA 
GAAAGCGAGA GAAAGCTCTT TAAATGTCCC AAATGCTTGG TGGACTTAGA AACCTCCAAC 
AAACTTTTTG AAGCTCAACA CGAAGTAGTT GAAAAGAATG ATCTTTTGAA TTTTGCATTA 
AACAGTGAAG AAGGTAGTAG AGACCGTTTC AAAGTCATAA CAGAGTTTTT AGGTAGAGGT 
GCCATCAGTT ATTCTGACAT CACTATTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_013958.1
CDS	1..3090
Translation	MSLSSWRQFQLFENIPIRDPNFGGDSLLYSDPTLCAATIVDPQTLIIAVNSNIIKVVKLNQSQVIHEFQSFPHDFQITFLKVINGEFLVALAESIGKPSLIRVYKLEKLPNREQLYHSQVELKNGNNTYPISVVSISNDLSCIVVGFINGKIILIRGDISRDRGSQQRIIYEDPSKEPITALFLNNDATACFAATTSRILLFNTTGRNRGRPSLVLNSKNGLDLNCGSFNPATNEFICCLSNFIEFFSSSGKKHQFAFDLSLRKRIFCVDKDHILIVTEETGVPTTSISVNELSPTIINRIFIIDAKNKIISLNFVVSSAIIDIFSTSQSGKNITYLLTSEGVMHRITPKSLENQINIIIQKELYPFALQLAKQHSLSPLDVQEIHKKYGDYLFKKGLRKEATDQYIQCLDVVETSEIISKFGVKEVPDPESMRNLADYLWSLIKNSISQRDHVTLLLIVLIKLKDVEGIDTFIQHFDRKGIWNEGVVMDDMDDVTFFYSDNDFFDLDLILELMKESDFKRLSYRLAKKYSKDSLIIVDILLNLLHNPVKAIKYIKSLPIDETLRCLVTYSKKLLEESPNETNALLIEVFTGKFKPSTFEVDLDRRDTTGDFSENIRTVFYSYKTFFNYMNSNGTSDAMSESSEASHEHEEPTYHPPKPSIVFSSFVTKPFEFVVFLEACLACYQQYEGFDEDRQVILTTLYDLYLNLAQNDVPERIDDWRSRATGVLRESNKLVYSAASNNTSKRVDNSIMLLISHMDQSSASAKDKTKIDIASFANDNPEMDLLSTFRAMTLNEEPSTCLKFLEKYGTEEPKLLQVALSYFVSNKLIFKEMGGNEVLKEKVLRPIIEGERMPLLDIIKALSRTNVAHFGLIQDIIIDHVKTEDTEIKRNEKLIESYDKELKEKNKKLKNTINSDQPLHVPLKNQTCFMCRLTLDIPVVFFKCGHIYHQHCLNEEEDTLESERKLFKCPKCLVDLETSNKLFEAQHEVVEKNDLLNFALNSEEGSRDRFKVITEFLGRGAISYSDITI

Target ORF information:

RefSeq Version	NM_001182738.1
Organism	Saccharomyces cerevisiae S288C
Definition	Saccharomyces cerevisiae S288C tethering complex subunit PEP5 (PEP5), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001182738.1

ORF Insert Sequence:

ATGTCCCTGA GCTCCTGGAG GCAATTCCAG CTTTTCGAGA ATATTCCCAT AAGAGATCCT 
AATTTTGGAG GAGATTCCCT ATTGTACTCG GACCCAACAC TTTGCGCAGC TACAATAGTA 
GATCCTCAAA CTCTGATCAT AGCTGTAAAT TCAAATATTA TAAAAGTTGT CAAATTAAAT 
CAATCGCAAG TAATACATGA ATTTCAATCT TTTCCTCATG ATTTTCAAAT CACTTTCCTG 
AAAGTCATCA ACGGGGAATT TCTCGTAGCG TTAGCTGAAT CCATTGGCAA ACCTTCCCTA 
ATAAGAGTAT ATAAACTAGA AAAACTGCCA AATAGAGAAC AACTATACCA TTCACAAGTC 
GAACTGAAAA ACGGTAATAA TACATACCCT ATTTCAGTTG TCTCCATATC AAATGACCTT 
TCCTGTATTG TGGTTGGATT CATTAATGGG AAAATCATCC TTATTAGAGG TGACATTTCA 
AGAGATAGAG GATCTCAACA AAGGATTATA TATGAAGATC CAAGTAAAGA GCCAATAACA 
GCTTTATTCC TCAATAACGA CGCAACGGCT TGTTTTGCGG CTACAACTTC AAGAATCCTT 
TTATTTAATA CGACTGGTAG AAATAGAGGG CGCCCAAGTT TGGTTTTAAA TTCAAAAAAT 
GGCCTGGATC TAAATTGTGG ATCTTTTAAT CCGGCAACAA ACGAATTTAT ATGTTGCTTA 
AGCAACTTTA TCGAATTTTT CAGCTCTAGT GGAAAAAAGC ATCAATTCGC ATTTGATCTA 
TCGCTGAGAA AGAGGATATT TTGTGTAGAT AAAGATCACA TTTTGATTGT GACTGAGGAA 
ACAGGTGTAC CAACTACATC CATAAGTGTT AATGAACTGT CGCCCACAAT AATTAACCGA 
ATATTCATCA TTGATGCTAA AAATAAAATC ATTTCTTTGA ATTTTGTTGT TTCTAGTGCA 
ATTATTGATA TTTTTTCAAC CTCTCAGAGC GGTAAAAACA TCACTTATCT GCTAACTTCT 
GAAGGAGTAA TGCATAGGAT AACTCCAAAA TCTTTAGAAA ATCAAATTAA CATCATCATC 
CAAAAGGAAC TGTACCCTTT TGCTTTACAA TTAGCGAAAC AGCATTCATT ATCACCGCTA 
GATGTTCAGG AAATTCATAA AAAATATGGT GATTATCTCT TTAAAAAGGG ACTCAGGAAG 
GAAGCAACAG ACCAGTACAT ACAATGTCTG GACGTTGTGG AAACTAGTGA AATCATTTCA 
AAATTTGGTG TCAAGGAGGT TCCTGACCCA GAGAGTATGA GGAACTTGGC TGACTATCTG 
TGGTCTTTGA TCAAGAATTC TATTTCTCAA CGCGATCATG TTACCCTTTT GTTAATTGTT 
TTAATCAAAT TGAAGGATGT TGAAGGAATT GATACTTTTA TCCAGCATTT CGATAGAAAA 
GGCATTTGGA ATGAAGGTGT TGTGATGGAC GATATGGATG ATGTGACGTT TTTTTATTCA 
GATAACGATT TTTTTGATTT AGATTTAATC TTAGAGCTTA TGAAAGAGTC AGATTTTAAG 
CGTCTCTCAT ACAGACTTGC AAAGAAGTAT TCAAAGGATT CATTAATTAT TGTGGATATT 
TTATTGAATC TATTGCATAA CCCTGTAAAA GCCATAAAAT ACATAAAAAG TTTACCAATT 
GATGAAACCC TAAGATGCCT TGTGACGTAC TCGAAAAAAT TACTGGAGGA GTCACCGAAT 
GAAACTAATG CTTTACTGAT AGAAGTATTT ACAGGGAAGT TCAAACCATC CACTTTTGAA 
GTGGATCTGG ACCGGAGAGA TACGACGGGC GATTTTTCTG AAAATATTAG AACAGTTTTT 
TACAGTTACA AAACATTCTT CAACTACATG AACTCAAATG GTACATCAGA CGCAATGAGC 
GAGTCTTCAG AGGCATCCCA CGAACATGAA GAGCCTACTT ATCATCCGCC GAAGCCATCC 
ATTGTTTTCA GTTCATTCGT TACAAAACCT TTTGAATTTG TTGTTTTCTT AGAAGCCTGT 
TTGGCATGTT ACCAGCAGTA TGAGGGTTTT GACGAAGATA GGCAAGTAAT TTTGACTACT 
TTATATGACT TATATTTGAA TTTGGCGCAA AATGATGTAC CCGAGCGTAT AGACGACTGG 
CGTTCAAGAG CAACTGGCGT ATTGCGTGAG AGCAATAAAT TGGTGTATTC TGCCGCAAGT 
AATAATACTA GCAAAAGGGT GGATAACTCA ATAATGCTGT TAATTTCCCA TATGGATCAA 
AGTAGTGCTT CAGCAAAAGA TAAGACGAAA ATTGACATAG CTTCATTTGC CAATGATAAC 
CCCGAGATGG ATTTGCTGAG TACATTTAGG GCTATGACGT TAAATGAAGA ACCAAGTACT 
TGTCTCAAAT TCCTAGAAAA ATATGGCACA GAGGAACCCA AGCTCTTACA AGTAGCATTG 
AGTTACTTTG TTTCTAATAA ACTAATCTTC AAGGAGATGG GCGGTAATGA AGTACTGAAA 
GAAAAAGTAT TGAGGCCAAT TATAGAGGGG GAAAGAATGC CACTGTTGGA TATAATTAAA 
GCGCTATCCC GTACAAATGT AGCCCACTTT GGGCTGATAC AAGACATCAT TATTGATCAT 
GTCAAAACCG AAGATACAGA AATCAAAAGG AACGAAAAAC TAATTGAATC TTACGATAAA 
GAGTTAAAGG AGAAAAACAA GAAGTTGAAG AACACCATTA ATTCAGATCA ACCTCTCCAC 
GTACCCCTGA AGAATCAAAC GTGTTTCATG TGTAGACTGA CATTGGATAT TCCTGTAGTT 
TTTTTTAAAT GTGGTCACAT TTACCACCAA CATTGTCTAA ATGAGGAAGA AGATACTCTA 
GAAAGCGAGA GAAAGCTCTT TAAATGTCCC AAATGCTTGG TGGACTTAGA AACCTCCAAC 
AAACTTTTTG AAGCTCAACA CGAAGTAGTT GAAAAGAATG ATCTTTTGAA TTTTGCATTA 
AACAGTGAAG AAGGTAGTAG AGACCGTTTC AAAGTCATAA CAGAGTTTTT AGGTAGAGGT 
GCCATCAGTT ATTCTGACAT CACTATTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The nucleotide sequence of Saccharomyces cerevisiae chromosome XIII.
Nature387(6632 Suppl)90-3(1997 May)
Bowman S,Churcher C,Badcock K,Brown D,Chillingworth T,Connor R,Dedman K,Devlin K,Gentles S,Hamlin N,Hunt S,Jagels K,Lye G,Moule S,Odell C,Pearson D,Rajandream M,Rice P,Skelton J,Walsh S,Whitehead S,Barrell B

Life with 6000 genes.
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)
Goffeau A,Barrell BG,Bussey H,Davis RW,Dujon B,Feldmann H,Galibert F,Hoheisel JD,Jacq C,Johnston M,Louis EJ,Mewes HW,Murakami Y,Philippsen P,Tettelin H,Oliver SG

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PEP5 cDNA ORF clone, Saccharomyces cerevisiae S288C

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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