SIM1 cDNA ORF clone, Saccharomyces cerevisiae S288C

The following SIM1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the SIM1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OSi03719 NM_001179471.2
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Saccharomyces cerevisiae S288C putative glucosidase SIM1 (SIM1), partial mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $258.30
$369.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OSi03719
    Clone ID Related Accession (Same CDS sequence) NM_001179471.2
    Accession Version NM_001179471.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1431bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-10-31
    Organism Saccharomyces cerevisiae S288C
    Product putative glucosidase SIM1
    Comment Comment: REVIEWED REFSEQ: This record has been curated by SGD. This record is derived from an annotated genomic sequence (NC_001141). On Apr 26, 2011 this sequence version replaced NM_001179471.1. ##Genome-Annotation-Data-START## Annotation Provider :: SGD Annotation Status :: Full Annotation Annotation Version :: R64-2-1 URL :: http://www.yeastgenome.org/ ##Genome-Annotation-Data-END## COMPLETENESS: incomplete on both ends.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    ATGAAATTCT CAACTGCCGT TACTACGTTG ATTAGTTCTG GTGCCATCGT GTCTGCTTTA 
    CCACACGTGG ATGTTCACCA AGAAGATGCC CACCAACATA AGAGGGCCGT TGCGTACAAA
    TACGTTTACG AAACTGTTGT TGTCGATTCT GATGGCCACA CTGTAACTCC TGCTGCTTCA
    GAAGTCGCTA CTGCTGCTAC CTCTGCTATC ATTACAACAT CTGTGTTGGC TCCAACCTCC
    TCCGCAGCCG CTGCGGATAG CTCCGCTTCC ATTGCTGTTT CATCTGCTGC CTTAGCCAAG
    AATGAGAAAA TCTCTGATGC CGCTGCATCT GCCACTGCCT CAACATCTCA AGGGGCATCC
    TCCTCCTCCT CCTCCTCCTC GGCAACTTCT ACCCTAGAAA GCAGCTCTGT TTCTTCATCT
    AGTGAAGAAG CTGCTCCAAC ATCTACTGTC GTGTCAACTT CTTCCGCAAC CCAATCTAGT
    GCTTCTTCTG CCACTAAATC TAGTACTTCT TCCACTTCAC CATCTACTTC TACTTCTACT
    TCCACTTCTT CTACTTCCTC TTCCTCTTCC TCCTCCTCCT CCTCTTCTTC TTCTTCTTCT
    GGCAGTGGTA GTATCTACGG TGATTTGGCC GACTTTTCAG GCCCAAGTGA GAAATTCCAA
    GACGGCACTA TTCCATGTGA CAAATTCCCA TCTGGTCAAG GTGTCATTTC TATTGACTGG
    ATTGGCGAGG GTGGATGGTC CGGTGTGGAA AACACCGACA CTTCCACTGG CGGTTCATGC
    AAGGAGGGGT CCTACTGTTC CTACTCCTGC CAACCAGGTA TGTCTAAGAC CCAATGGCCA
    TCCGATCAAC CATCTGACGG TAGATCTGTC GGGGGTTTGT TGTGTAAAAA TGGTTATTTG
    TACCGTTCTA ACACTGACGC GGATTACTTA TGTGAATGGG GTGTCGAGGC TGCCTATGTT
    GTTTCTAAAC TAAGCAAGGG TGTCGCCATT TGCAGAACCG ACTACCCGGG CACTGAAAAC
    ATGGTTATCC CAACCTATGT TGAAGGGGGT AGCTCTTTGC CATTGACCGT TGTTGACCAA
    GATACTTACT TTACTTGGGA AGGCAAAAAG ACATCTGCTC AATACTACGT TAATAACGCC
    GGCGTCTCAG TTGAAGATGG GTGTATCTGG GGTACTTCTG GATCTGGTAT TGGTAACTGG
    GCACCATTAA ACTTTGGTGC TGGCTCCACT GGTGGAGTGA CATACTTATC ATTGATTCCT
    AACCCAAACA ACAGCGACGC ATTGAACTAC AACGTCAAGA TAGTTGCTGC TGATGATTCA
    TCCAATGTCA TCGGTGAATG TGTTTACGAA AATGGTGAGT TCTCTGGCGG TGCTGACGGG
    TGTACCGTCT CTGTTACTTC CGGTAAAGCT CATTTCGTCT TATACAATTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_012143.2
    CDS1..1431
    Translation

    Target ORF information:

    RefSeq Version NM_001179471.2
    Organism Saccharomyces cerevisiae S288C
    Definition Saccharomyces cerevisiae S288C putative glucosidase SIM1 (SIM1), partial mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001179471.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    ATGAAATTCT CAACTGCCGT TACTACGTTG ATTAGTTCTG GTGCCATCGT GTCTGCTTTA 
    CCACACGTGG ATGTTCACCA AGAAGATGCC CACCAACATA AGAGGGCCGT TGCGTACAAA
    TACGTTTACG AAACTGTTGT TGTCGATTCT GATGGCCACA CTGTAACTCC TGCTGCTTCA
    GAAGTCGCTA CTGCTGCTAC CTCTGCTATC ATTACAACAT CTGTGTTGGC TCCAACCTCC
    TCCGCAGCCG CTGCGGATAG CTCCGCTTCC ATTGCTGTTT CATCTGCTGC CTTAGCCAAG
    AATGAGAAAA TCTCTGATGC CGCTGCATCT GCCACTGCCT CAACATCTCA AGGGGCATCC
    TCCTCCTCCT CCTCCTCCTC GGCAACTTCT ACCCTAGAAA GCAGCTCTGT TTCTTCATCT
    AGTGAAGAAG CTGCTCCAAC ATCTACTGTC GTGTCAACTT CTTCCGCAAC CCAATCTAGT
    GCTTCTTCTG CCACTAAATC TAGTACTTCT TCCACTTCAC CATCTACTTC TACTTCTACT
    TCCACTTCTT CTACTTCCTC TTCCTCTTCC TCCTCCTCCT CCTCTTCTTC TTCTTCTTCT
    GGCAGTGGTA GTATCTACGG TGATTTGGCC GACTTTTCAG GCCCAAGTGA GAAATTCCAA
    GACGGCACTA TTCCATGTGA CAAATTCCCA TCTGGTCAAG GTGTCATTTC TATTGACTGG
    ATTGGCGAGG GTGGATGGTC CGGTGTGGAA AACACCGACA CTTCCACTGG CGGTTCATGC
    AAGGAGGGGT CCTACTGTTC CTACTCCTGC CAACCAGGTA TGTCTAAGAC CCAATGGCCA
    TCCGATCAAC CATCTGACGG TAGATCTGTC GGGGGTTTGT TGTGTAAAAA TGGTTATTTG
    TACCGTTCTA ACACTGACGC GGATTACTTA TGTGAATGGG GTGTCGAGGC TGCCTATGTT
    GTTTCTAAAC TAAGCAAGGG TGTCGCCATT TGCAGAACCG ACTACCCGGG CACTGAAAAC
    ATGGTTATCC CAACCTATGT TGAAGGGGGT AGCTCTTTGC CATTGACCGT TGTTGACCAA
    GATACTTACT TTACTTGGGA AGGCAAAAAG ACATCTGCTC AATACTACGT TAATAACGCC
    GGCGTCTCAG TTGAAGATGG GTGTATCTGG GGTACTTCTG GATCTGGTAT TGGTAACTGG
    GCACCATTAA ACTTTGGTGC TGGCTCCACT GGTGGAGTGA CATACTTATC ATTGATTCCT
    AACCCAAACA ACAGCGACGC ATTGAACTAC AACGTCAAGA TAGTTGCTGC TGATGATTCA
    TCCAATGTCA TCGGTGAATG TGTTTACGAA AATGGTGAGT TCTCTGGCGG TGCTGACGGG
    TGTACCGTCT CTGTTACTTC CGGTAAAGCT CATTTCGTCT TATACAATTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

  • PubMed

    The nucleotide sequence of Saccharomyces cerevisiae chromosome IX.
    Nature387(6632 Suppl)84-7(1997 May)
    Churcher C,Bowman S,Badcock K,Bankier A,Brown D,Chillingworth T,Connor R,Devlin K,Gentles S,Hamlin N,Harris D,Horsnell T,Hunt S,Jagels K,Jones M,Lye G,Moule S,Odell C,Pearson D,Rajandream M,Rice P,Rowley N,Skelton J,Smith V,Barrell B


    Life with 6000 genes.
    Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)
    Goffeau A,Barrell BG,Bussey H,Davis RW,Dujon B,Feldmann H,Galibert F,Hoheisel JD,Jacq C,Johnston M,Louis EJ,Mewes HW,Murakami Y,Philippsen P,Tettelin H,Oliver SG