MET18 cDNA ORF clone, Saccharomyces cerevisiae S288C

Gene
Clones
gRNAs

Summary

Gene Symbol	MET18
Entrez Gene ID	854678
Full Name	Met18p
Synonyms	MMS19
Gene Type	protein-coding
Organism	Saccharomyces cerevisiae S288C

mRNA and Protein(s)

mRNA	Protein	Name
NM_001179476.1	NP_012138.1	Met18p

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	MMS19	XP_001162859.2
Gallus gallus (chicken)	MMS19	XP_421710.4
Schizosaccharomyces pombe (fission yeast)	mms19	NP_594352.1
Homo sapiens (human)	MMS19	NP_071757.4
	MMS19	XP_002808554.1
Canis lupus familiaris (dog)	MMS19	XP_005637645.1
Rattus norvegicus (Norway rat)	Mms19l	NP_001258525.1
Danio rerio (zebrafish)	mms19	XP_005156048.1
Kluyveromyces lactis	KLLA0F04565g	XP_455286.1
Saccharomyces cerevisiae (baker's yeast)	MET18	NP_012138.1
Eremothecium gossypii	AGOS_AER377C	NP_985233.2
Mus musculus (house mouse)	Mms19	NP_082428.1
Xenopus tropicalis (tropical clawed frog)	mms19	NP_001072380.1

Gene Ontology
Bibliography

Related articles in PubMed

The nucleotide sequence of Saccharomyces cerevisiae chromosome IX.
Churcher C, Bowman S, Badcock K, Bankier A, Brown D, Chillingworth T, Connor R, Devlin K, Gentles S, Hamlin N, Harris D, Horsnell T, Hunt S, Jagels K, Jones M, Lye G, Moule S, Odell C, Pearson D, Rajandream M, Rice P, Rowley N, Skelton J, Smith V, Barrell B
Nature387(6632 Suppl)84-7(1997 May)

Life with 6000 genes.
Goffeau A, Barrell BG, Bussey H, Davis RW, Dujon B, Feldmann H, Galibert F, Hoheisel JD, Jacq C, Johnston M, Louis EJ, Mewes HW, Murakami Y, Philippsen P, Tettelin H, Oliver SG
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

identified MMS19 as a member of the cytosolic iron-sulfur protein assembly (CIA) machinery; MMS19 functions as part of the CIA targeting complex that interacts with and facilitates iron-sulfur cluster insertion into apoproteins involved in methionine biosynthesis, DNA replication, DNA repair, and telomere maintenance
Title: MMS19 assembles iron-sulfur proteins required for DNA metabolism and genomic integrity.

The following MET18 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the MET18 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OSi03714	NM_001179476.1 Latest version!	Saccharomyces cerevisiae S288C Met18p (MET18), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

MET18 ( NM_001179476.1 ) cDNA ORF clone, Saccharomyces cerevisiae S288C -> NP_012138.1 Saccharomyces cerevisiae S288C Met18p (MET18), partial mRNA.

Price & Availability↑

CloneID	OSi03714
Clone ID Related Accession (Same CDS sequence)	NM_001179476.1
Accession Version	NM_001179476.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3099bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-31
Organism	Saccharomyces cerevisiae S288C
Product	Met18p
Comment	Comment: REVIEWED REFSEQ: This record has been curated by SGD. This record is derived from an annotated genomic sequence (NC_001141). ##Genome-Annotation-Data-START## Annotation Provider :: SGD Annotation Status :: Full Annotation Annotation Version :: R64-2-1 URL :: http://www.yeastgenome.org/ ##Genome-Annotation-Data-END## COMPLETENESS: incomplete on both ends.

ATGACACCAG ACGAACTAAA TTCAGCAGTC GTCACCTTTA TGGCCAATCT GAATATTGAT 
GATTCGAAGG CCAACGAAAC CGCTTCCACA GTAACGGATT CTATTGTACA CCGATCAATA 
AAATTGCTGG AAGTTGTCGT TGCGTTGAAA GATTATTTTC TTTCAGAAAA TGAGGTAGAA 
AGGAAGAAAG CGTTGACATG TTTAACCACT ATCTTAGCAA AAACTCCCAA GGACCACCTC 
TCCAAAAACG AATGCTCAGT AATATTTCAG TTTTACCAGT CTAAGCTTGA TGATCAAGCG 
CTTGCAAAAG AAGTCTTGGA GGGCTTCGCC GCATTAGCTC CAATGAAATA TGTCTCCATT 
AACGAGATAG CCCAACTTTT GAGATTATTA TTGGACAACT ATCAACAAGG CCAGCATTTG 
GCATCAACTA GACTGTGGCC ATTTAAAATT TTGAGAAAAA TATTTGATAG ATTTTTCGTC 
AATGGATCCT CGACCGAACA AGTCAAACGA ATCAACGATT TGTTTATTGA AACATTTCTT 
CATGTTGCCA ATGGTGAAAA AGACCCTAGA AATTTACTCT TGTCATTTGC ACTTAATAAA 
TCTATCACAT CGTCCTTGCA AAACGTGGAG AATTTTAAAG AAGACTTATT TGATGTCTTA 
TTTTGCTATT TCCCAATCAC TTTCAAGCCA CCAAAGCATG ATCCTTACAA GATCTCCAAC 
CAAGATTTAA AAACAGCTTT GCGATCAGCA ATTACTGCGA CGCCATTATT TGCCGAAGAT 
GCTTATAGCA ATTTGCTGGA CAAACTCACT GCTTCTTCAC CGGTAGTGAA AAATGATACA 
CTTCTGACGC TATTAGAATG CGTTAGAAAA TTCGGCGGCT CTTCTATATT GGAAAATTGG 
ACGTTACTTT GGAACGCTTT GAAATTTGAA ATTATGCAAA ACAGCGAAGG AAATGAAAAC 
ACTCTACTAA ATCCGTACAA TAAAGACCAA CAGAGTGATG ATGTGGGCCA ATATACTAAC 
TACGATGCTT GCTTGAAAAT TATTAATCTA ATGGCATTGC AACTATACAA TTTTGACAAA 
GTATCATTTG AAAAGTTCTT CACTCATGTA TTAGATGAGT TGAAACCAAA TTTTAAATAT 
GAAAAGGATT TGAAGCAAAC CTGCCAGATT TTGTCCGCTA TAGGTAGTGG GAATGTCGAG 
ATATTTAACA AGGTCATTTC GTCAACTTTC CCCTTATTTT TGATCAATAC ATCTGAAGTT 
GCCAAACTTA AGCTGCTGAT TATGAATTTT TCTTTTTTTG TTGACTCGTA CATTGATTTG 
TTTGGAAGAA CATCTAAAGA ATCGTTGGGA ACACCGGTGC CAAATAATAA AATGGCTGAA 
TACAAAGACG AGATCATAAT GATTTTGAGC ATGGCCTTGA CAAGAAGCTC CAAGGCAGAG 
GTTACCATAA GGACTCTATC TGTGATTCAA TTCACAAAAA TGATTAAAAT GAAGGGCTTT 
TTAACCCCAG AGGAAGTTTC ACTAATTATT CAATACTTCA CGGAAGAAAT ATTAACGGAT 
AACAACAAAA ACATTTACTA TGCTTGTTTG GAAGGGTTAA AGACAATCAG TGAGATATAT 
GAGGATTTAG TTTTTGAAAT ATCATTGAAG AAATTATTGG ATTTGTTACC TGATTGCTTC 
GAGGAAAAAA TTCGAGTAAA TGATGAGGAA AATATTCACA TTGAAACAAT TTTGAAGATT 
ATTCTTGATT TCACCACTTC GAGACATATT TTGGTCAAAG AAAGCATTAC ATTTTTAGCG 
ACAAAATTGA ATAGGGTAGC TAAAATTTCG AAATCCAGAG AATATTGCTT TCTACTTATT 
TCAACCATAT ATTCTCTTTT TAATAATAAT AATCAAAATG AGAACGTGCT AAATGAAGAA 
GATGCTCTTG CACTCAAGAA TGCCATTGAG CCAAAATTAT TTGAGATAAT TACTCAGGAA 
TCGGCCATTG TAAGTGACAA TTACAATTTG ACACTTCTAT CTAACGTTCT TTTCTTCACC 
AATTTGAAAA TTCCACAGGC TGCACACCAA GAAGAACTAG ACAGATATAA CGAGCTTTTC 
ATTTCTGAAG GTAAGATAAG AATCTTAGAC ACACCGAATG TATTGGCTAT TTCATATGCT 
AAAATATTAT CCGCACTAAA TAAAAACTGC CAATTCCCCC AAAAATTTAC AGTGCTTTTC 
GGAACGGTTC AACTACTCAA AAAACATGCT CCAAGAATGA CGGAGACTGA AAAATTAGGA 
TACCTGGAAT TGCTGTTGGT CTTATCAAAT AAATTCGTCT CTGAAAAAGA TGTGATAGGA 
TTATTCGATT GGAAAGACTT ATCAGTAATT AATCTTGAAG TGATGGTTTG GTTGACTAAA 
GGGCTGATAA TGCAAAACTC ATTAGAGTCA TCAGAAATTG CAAAAAAGTT TATTGATTTA 
CTGTCTAATG AAGAGATTGG CTCACTTGTA TCGAAGCTAT TCGAAGTTTT TGTCATGGAT 
ATCAGTTCTT TGAAGAAATT TAAAGGTATC AGTTGGAATA ATAACGTTAA AATCTTGTAC 
AAACAAAAAT TCTTCGGTGA CATTTTTCAA ACACTAGTCA GCAACTATAA AAATACCGTG 
GACATGACCA TTAAATGCAA TTATTTAACT GCATTATCCT TAGTGTTGAA GCATACTCCA 
AGCCAATCGG TTGGTCCTTT TATCAACGAT TTATTCCCGC TTTTATTGCA GGCTTTGGAT 
ATGCCTGATC CTGAAGTAAG GGTATCCGCT TTAGAAACTC TGAAGGACAC TACGGATAAA 
CATCACACTT TAATTACTGA ACACGTAAGC ACAATAGTTC CATTATTACT ATCTCTTTCG 
CTTCCGCACA AATATAATAG TGTCAGTGTC AGGTTAATAG CCTTACAGCT TCTAGAAATG 
ATTACGACTG TAGTACCTCT AAACTATTGT TTGAGCTATC AGGACGATGT TCTAAGCGCT 
TTAATACCAG TTTTATCTGA CAAAAAAAGA ATAATAAGAA AGCAATGCGT TGACACAAGA 
CAGGTTTATT ATGAATTAGG CCAAATCCCG TTCGAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_012138.1
CDS	1..3099
Translation	MTPDELNSAVVTFMANLNIDDSKANETASTVTDSIVHRSIKLLEVVVALKDYFLSENEVERKKALTCLTTILAKTPKDHLSKNECSVIFQFYQSKLDDQALAKEVLEGFAALAPMKYVSINEIAQLLRLLLDNYQQGQHLASTRLWPFKILRKIFDRFFVNGSSTEQVKRINDLFIETFLHVANGEKDPRNLLLSFALNKSITSSLQNVENFKEDLFDVLFCYFPITFKPPKHDPYKISNQDLKTALRSAITATPLFAEDAYSNLLDKLTASSPVVKNDTLLTLLECVRKFGGSSILENWTLLWNALKFEIMQNSEGNENTLLNPYNKDQQSDDVGQYTNYDACLKIINLMALQLYNFDKVSFEKFFTHVLDELKPNFKYEKDLKQTCQILSAIGSGNVEIFNKVISSTFPLFLINTSEVAKLKLLIMNFSFFVDSYIDLFGRTSKESLGTPVPNNKMAEYKDEIIMILSMALTRSSKAEVTIRTLSVIQFTKMIKMKGFLTPEEVSLIIQYFTEEILTDNNKNIYYACLEGLKTISEIYEDLVFEISLKKLLDLLPDCFEEKIRVNDEENIHIETILKIILDFTTSRHILVKESITFLATKLNRVAKISKSREYCFLLISTIYSLFNNNNQNENVLNEEDALALKNAIEPKLFEIITQESAIVSDNYNLTLLSNVLFFTNLKIPQAAHQEELDRYNELFISEGKIRILDTPNVLAISYAKILSALNKNCQFPQKFTVLFGTVQLLKKHAPRMTETEKLGYLELLLVLSNKFVSEKDVIGLFDWKDLSVINLEVMVWLTKGLIMQNSLESSEIAKKFIDLLSNEEIGSLVSKLFEVFVMDISSLKKFKGISWNNNVKILYKQKFFGDIFQTLVSNYKNTVDMTIKCNYLTALSLVLKHTPSQSVGPFINDLFPLLLQALDMPDPEVRVSALETLKDTTDKHHTLITEHVSTIVPLLLSLSLPHKYNSVSVRLIALQLLEMITTVVPLNYCLSYQDDVLSALIPVLSDKKRIIRKQCVDTRQVYYELGQIPFE

Target ORF information:

RefSeq Version	NM_001179476.1
Organism	Saccharomyces cerevisiae S288C
Definition	Saccharomyces cerevisiae S288C Met18p (MET18), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001179476.1

ORF Insert Sequence:

ATGACACCAG ACGAACTAAA TTCAGCAGTC GTCACCTTTA TGGCCAATCT GAATATTGAT 
GATTCGAAGG CCAACGAAAC CGCTTCCACA GTAACGGATT CTATTGTACA CCGATCAATA 
AAATTGCTGG AAGTTGTCGT TGCGTTGAAA GATTATTTTC TTTCAGAAAA TGAGGTAGAA 
AGGAAGAAAG CGTTGACATG TTTAACCACT ATCTTAGCAA AAACTCCCAA GGACCACCTC 
TCCAAAAACG AATGCTCAGT AATATTTCAG TTTTACCAGT CTAAGCTTGA TGATCAAGCG 
CTTGCAAAAG AAGTCTTGGA GGGCTTCGCC GCATTAGCTC CAATGAAATA TGTCTCCATT 
AACGAGATAG CCCAACTTTT GAGATTATTA TTGGACAACT ATCAACAAGG CCAGCATTTG 
GCATCAACTA GACTGTGGCC ATTTAAAATT TTGAGAAAAA TATTTGATAG ATTTTTCGTC 
AATGGATCCT CGACCGAACA AGTCAAACGA ATCAACGATT TGTTTATTGA AACATTTCTT 
CATGTTGCCA ATGGTGAAAA AGACCCTAGA AATTTACTCT TGTCATTTGC ACTTAATAAA 
TCTATCACAT CGTCCTTGCA AAACGTGGAG AATTTTAAAG AAGACTTATT TGATGTCTTA 
TTTTGCTATT TCCCAATCAC TTTCAAGCCA CCAAAGCATG ATCCTTACAA GATCTCCAAC 
CAAGATTTAA AAACAGCTTT GCGATCAGCA ATTACTGCGA CGCCATTATT TGCCGAAGAT 
GCTTATAGCA ATTTGCTGGA CAAACTCACT GCTTCTTCAC CGGTAGTGAA AAATGATACA 
CTTCTGACGC TATTAGAATG CGTTAGAAAA TTCGGCGGCT CTTCTATATT GGAAAATTGG 
ACGTTACTTT GGAACGCTTT GAAATTTGAA ATTATGCAAA ACAGCGAAGG AAATGAAAAC 
ACTCTACTAA ATCCGTACAA TAAAGACCAA CAGAGTGATG ATGTGGGCCA ATATACTAAC 
TACGATGCTT GCTTGAAAAT TATTAATCTA ATGGCATTGC AACTATACAA TTTTGACAAA 
GTATCATTTG AAAAGTTCTT CACTCATGTA TTAGATGAGT TGAAACCAAA TTTTAAATAT 
GAAAAGGATT TGAAGCAAAC CTGCCAGATT TTGTCCGCTA TAGGTAGTGG GAATGTCGAG 
ATATTTAACA AGGTCATTTC GTCAACTTTC CCCTTATTTT TGATCAATAC ATCTGAAGTT 
GCCAAACTTA AGCTGCTGAT TATGAATTTT TCTTTTTTTG TTGACTCGTA CATTGATTTG 
TTTGGAAGAA CATCTAAAGA ATCGTTGGGA ACACCGGTGC CAAATAATAA AATGGCTGAA 
TACAAAGACG AGATCATAAT GATTTTGAGC ATGGCCTTGA CAAGAAGCTC CAAGGCAGAG 
GTTACCATAA GGACTCTATC TGTGATTCAA TTCACAAAAA TGATTAAAAT GAAGGGCTTT 
TTAACCCCAG AGGAAGTTTC ACTAATTATT CAATACTTCA CGGAAGAAAT ATTAACGGAT 
AACAACAAAA ACATTTACTA TGCTTGTTTG GAAGGGTTAA AGACAATCAG TGAGATATAT 
GAGGATTTAG TTTTTGAAAT ATCATTGAAG AAATTATTGG ATTTGTTACC TGATTGCTTC 
GAGGAAAAAA TTCGAGTAAA TGATGAGGAA AATATTCACA TTGAAACAAT TTTGAAGATT 
ATTCTTGATT TCACCACTTC GAGACATATT TTGGTCAAAG AAAGCATTAC ATTTTTAGCG 
ACAAAATTGA ATAGGGTAGC TAAAATTTCG AAATCCAGAG AATATTGCTT TCTACTTATT 
TCAACCATAT ATTCTCTTTT TAATAATAAT AATCAAAATG AGAACGTGCT AAATGAAGAA 
GATGCTCTTG CACTCAAGAA TGCCATTGAG CCAAAATTAT TTGAGATAAT TACTCAGGAA 
TCGGCCATTG TAAGTGACAA TTACAATTTG ACACTTCTAT CTAACGTTCT TTTCTTCACC 
AATTTGAAAA TTCCACAGGC TGCACACCAA GAAGAACTAG ACAGATATAA CGAGCTTTTC 
ATTTCTGAAG GTAAGATAAG AATCTTAGAC ACACCGAATG TATTGGCTAT TTCATATGCT 
AAAATATTAT CCGCACTAAA TAAAAACTGC CAATTCCCCC AAAAATTTAC AGTGCTTTTC 
GGAACGGTTC AACTACTCAA AAAACATGCT CCAAGAATGA CGGAGACTGA AAAATTAGGA 
TACCTGGAAT TGCTGTTGGT CTTATCAAAT AAATTCGTCT CTGAAAAAGA TGTGATAGGA 
TTATTCGATT GGAAAGACTT ATCAGTAATT AATCTTGAAG TGATGGTTTG GTTGACTAAA 
GGGCTGATAA TGCAAAACTC ATTAGAGTCA TCAGAAATTG CAAAAAAGTT TATTGATTTA 
CTGTCTAATG AAGAGATTGG CTCACTTGTA TCGAAGCTAT TCGAAGTTTT TGTCATGGAT 
ATCAGTTCTT TGAAGAAATT TAAAGGTATC AGTTGGAATA ATAACGTTAA AATCTTGTAC 
AAACAAAAAT TCTTCGGTGA CATTTTTCAA ACACTAGTCA GCAACTATAA AAATACCGTG 
GACATGACCA TTAAATGCAA TTATTTAACT GCATTATCCT TAGTGTTGAA GCATACTCCA 
AGCCAATCGG TTGGTCCTTT TATCAACGAT TTATTCCCGC TTTTATTGCA GGCTTTGGAT 
ATGCCTGATC CTGAAGTAAG GGTATCCGCT TTAGAAACTC TGAAGGACAC TACGGATAAA 
CATCACACTT TAATTACTGA ACACGTAAGC ACAATAGTTC CATTATTACT ATCTCTTTCG 
CTTCCGCACA AATATAATAG TGTCAGTGTC AGGTTAATAG CCTTACAGCT TCTAGAAATG 
ATTACGACTG TAGTACCTCT AAACTATTGT TTGAGCTATC AGGACGATGT TCTAAGCGCT 
TTAATACCAG TTTTATCTGA CAAAAAAAGA ATAATAAGAA AGCAATGCGT TGACACAAGA 
CAGGTTTATT ATGAATTAGG CCAAATCCCG TTCGAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The nucleotide sequence of Saccharomyces cerevisiae chromosome IX.
Nature387(6632 Suppl)84-7(1997 May)
Churcher C,Bowman S,Badcock K,Bankier A,Brown D,Chillingworth T,Connor R,Devlin K,Gentles S,Hamlin N,Harris D,Horsnell T,Hunt S,Jagels K,Jones M,Lye G,Moule S,Odell C,Pearson D,Rajandream M,Rice P,Rowley N,Skelton J,Smith V,Barrell B

Life with 6000 genes.
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)
Goffeau A,Barrell BG,Bussey H,Davis RW,Dujon B,Feldmann H,Galibert F,Hoheisel JD,Jacq C,Johnston M,Louis EJ,Mewes HW,Murakami Y,Philippsen P,Tettelin H,Oliver SG

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MET18 cDNA ORF clone, Saccharomyces cerevisiae S288C

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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