NUP1 cDNA ORF clone, Saccharomyces cerevisiae S288C

Gene
Clones
gRNAs

Summary

Gene Symbol	NUP1
Entrez Gene ID	854265
Full Name	FG-nucleoporin NUP1
Gene Type	protein-coding
Organism	Saccharomyces cerevisiae S288C

mRNA and Protein(s)

mRNA	Protein	Name
NM_001183517.1	NP_014741.1	FG-nucleoporin NUP1

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

The nucleotide sequence of Saccharomyces cerevisiae chromosome XV.
Dujon B, Albermann K, Aldea M, Alexandraki D, Ansorge W, Arino J, Benes V, Bohn C, Bolotin-Fukuhara M, Bordonn? R, Boyer J, Camasses A, Casamayor A, Casas C, Ch?ret G, Cziepluch C, Daignan-Fornier B, Dang DV, de Haan M, Delius H, Durand P, Fairhead C, Feldmann H, Gaillon L, Kleine K
Nature387(6632 Suppl)98-102(1997 May)

Life with 6000 genes.
Goffeau A, Barrell BG, Bussey H, Davis RW, Dujon B, Feldmann H, Galibert F, Hoheisel JD, Jacq C, Johnston M, Louis EJ, Mewes HW, Murakami Y, Philippsen P, Tettelin H, Oliver SG
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

high levels of the Nup1/Nup60 amphipathic helices cause deformation of the yeast nuclear membrane
Title: Nuclear pore basket proteins are tethered to the nuclear envelope and can regulate membrane curvature.

a novel functional connection between Nup1 and proteins comprising both the spindle pole body and early mitotic exit network
Title: Mutations affecting spindle pole body and mitotic exit network function are synthetically lethal with a deletion of the nucleoporin NUP1 in S. cerevisiae.

Although full-length Nup1p or Nup153 does not complement Nup124p, the functionality of their conserved domains with reference to Tf1 activity suggests that these three proteins evolved from a common ancestor.
Title: Multiple conserved domains of the nucleoporin Nup124p and its orthologs Nup1p and Nup153 are critical for nuclear import and activity of the fission yeast Tf1 retrotransposon.

Pom152 mutants lacking N-linked glycosylation sites are viable in combination with nup1Delta, suppress nup1Delta temperature sensitivity, and partially suppress the nuclear protein import defects associated with the deletion of NUP1.
Title: Nuclear pore complex function in Saccharomyces cerevisiae is influenced by glycosylation of the transmembrane nucleoporin Pom152p.

The following NUP1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the NUP1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OSi03403	NM_001183517.1 Latest version!	Saccharomyces cerevisiae S288C FG-nucleoporin NUP1 (NUP1), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

NUP1 ( NM_001183517.1 ) cDNA ORF clone, Saccharomyces cerevisiae S288C -> NP_014741.1 Saccharomyces cerevisiae S288C FG-nucleoporin NUP1 (NUP1), partial mRNA.

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CloneID	OSi03403
Clone ID Related Accession (Same CDS sequence)	NM_001183517.1
Accession Version	NM_001183517.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3231bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-31
Organism	Saccharomyces cerevisiae S288C
Product	FG-nucleoporin NUP1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by SGD. This record is derived from an annotated genomic sequence (NC_001147). ##Genome-Annotation-Data-START## Annotation Provider :: SGD Annotation Status :: Full Annotation Annotation Version :: R64-2-1 URL :: http://www.yeastgenome.org/ ##Genome-Annotation-Data-END## COMPLETENESS: incomplete on both ends.

ATGTCTTCAA ACACTTCTTC TGTGATGTCT TCTCCACGTG TCGAAAAGAG ATCGTTTTCT 
TCCACTTTAA AATCATTCTT CACAAACCCC AATAAAAAAC GGCCATCGAG CAAGAAAGTT 
TTCAGTTCAA ACCTCTCATA CGCGAATCAT TTGGAGGAAT CAGATGTTGA AGACACACTG 
CATGTTAATA AGAGAAAGAG GGTGTCCGGT ACATCACAGC ATAGCGACAG CTTAACTCAG 
AACAACAATA ATGCGCCAAT TATAATATAT GGAACCGAAA ACACTGAGAG ACCGCCGCTT 
TTGCCAATTT TGCCCATTCA AAGACTGAGG TTATTAAGGG AGAAGCAGAG GGTGAGAAAT 
ATGCGTGAGC TTGGATTAAT TCAATCAACT GAATTTCCAT CTATTACATC GTCGGTTATA 
TTGGGCTCTC AAAGTAAAAG CGATGAGGGA GGATCGTACC TATGCACATC ATCTACTCCT 
TCCCCTATTA AAAACGGTTC TTGCACTAGG CAGTTGGCCG GAAAAAGCGG TGAAGACACC 
AATGTTGGAC TACCCATTCT CAAATCATTG AAAAATAGAT CCAATAGAAA AAGGTTTCAT 
AGTCAGTCAA AGGGGACCGT GTGGTCAGCA AATTTTGAAT ATGATTTGTC AGAATATGAC 
GCTATACAAA AAAAGGATAA CAAGGATAAG GAAGGTAACG CTGGCGGTGA TCAGAAGACA 
AGCGAGAATA GAAATAATAT TAAGAGTAGT ATTTCAAATG GCAATCTGGC TACAGGCCCT 
AACCTGACAA GCGAAATTGA AGACCTACGT GCAGACATCA ACTCTAATAG GTTATCGAAT 
CCTCAAAAAA ATCTACTTTT AAAAGGACCA GCTTCCACAG TTGCAAAAAC TGCCCCTATT 
CAGGAGAGCT TTGTTCCCAA TTCAGAGCGC TCTGGTACGC CTACGTTAAA GAAAAATATT 
GAGCCCAAAA AGGACAAAGA AAGTATTGTT TTGCCCACCG TAGGTTTTGA CTTTATCAAG 
GACAATGAGA CTCCATCTAA GAAAACTTCT CCTAAGGCAA CTTCTTCTGC AGGTGCAGTC 
TTTAAATCGA GTGTAGAAAT GGGAAAAACC GATAAGTCAA CGAAAACTGC CGAGGCGCCT 
ACCTTATCAT TCAATTTTAG CCAAAAGGCT AATAAAACTA AGGCTGTCGA CAATACTGTC 
CCTTCCACAA CTTTATTCAA TTTTGGTGGT AAATCAGATA CCGTTACTTC TGCCAGTCAA 
CCTTTTAAAT TTGGAAAGAC ATCCGAAAAA AGTGAAAATC ATACAGAATC AGACGCGCCT 
CCAAAATCAA CTGCTCCAAT ATTTTCTTTT GGTAAACAAG AAGAGAATGG TGATGAAGGT 
GATGATGAAA ATGAGCCCAA AAGAAAAAGG CGTTTACCTG TTAGCGAGGA TACAAACACC 
AAGCCTTTAT TCGATTTCGG CAAGACCGGT GATCAAAAGG AGACCAAAAA GGGAGAGTCA 
GAAAAGGACG CATCAGGGAA ACCAAGCTTT GTCTTTGGTG CAAGTGATAA GCAAGCTGAA 
GGTACACCAT TATTTACATT CGGAAAAAAA GCTGATGTAA CAAGCAATAT TGACTCCTCT 
GCACAATTTA CCTTTGGTAA AGCCGCCACC GCGAAAGAAA CACACACCAA ACCATCTGAG 
ACACCTGCCA CAATAGTCAA GAAGCCTACT TTTACTTTTG GGCAGTCAAC AAGTGAAAAT 
AAGATCTCTG AGGGAAGTGC GAAACCTACA TTCTCTTTCT CTAAGTCAGA GGAGGAACGT 
AAGAGTAGTC CAATTTCAAA CGAAGCAGCT AAACCCTCGT TTTCGTTTCC GGGCAAGCCT 
GTTGATGTTC AAGCACCGAC GGATGATAAG ACTCTCAAGC CAACTTTTTC TTTTACTGAA 
CCTGCTCAAA AAGATTCATC TGTTGTTTCG GAACCTAAAA AGCCCTCCTT TACGTTTGCG 
TCTTCAAAAA CCTCACAACC AAAGCCATTG TTTTCATTTG GTAAGTCAGA TGCAGCTAAA 
GAACCACCAG GCTCTAACAC CTCATTTTCT TTCACTAAAC CTCCTGCTAA TGAGACAGAT 
AAAAGACCTA CACCGCCATC TTTCACCTTT GGCGGTTCCA CAACAAATAA TACAACAACC 
ACTAGCACAA AACCATCTTT TAGTTTTGGG GCTCCCGAGT CGATGAAGTC GACAGCAAGT 
ACAGCGGCAG CAAATACGGA GAAGCTATCA AATGGCTTTT CCTTTACAAA GTTCAATCAC 
AATAAAGAAA AGTCAAACTC TCCAACTTCT TTCTTCGATG GTTCTGCTTC CTCAACGCCG 
ATTCCTGTCT TGGGTAAGCC AACAGACGCT ACTGGTAATA CAACATCTAA ATCTGCATTT 
TCATTCGGTA CTGCTAACAC CAATGGTACC AATGCCTCAG CAAACTCCAC ATCATTCTCG 
TTTAACGCCC CTGCTACTGG TAACGGCACA ACTACTACTT CCAATACCTC AGGAACCAAT 
ATAGCGGGTA CATTTAACGT AGGAAAACCG GATCAAAGTA TCGCTAGTGG CAATACGAAT 
GGAGCGGGCT CGGCATTTGG CTTTTCGAGC TCAGGAACAG CAGCAACTGG TGCAGCTTCT 
AATCAATCTT CATTTAATTT TGGAAACAAT GGTGCAGGGG GTCTCAATCC TTTTACATCA 
GCAACTTCGT CAACTAATGC TAATGCTGGT TTATTCAATA AACCTCCTTC CACGAATGCA 
CAAAATGTCA ATGTTCCCTC TGCTTTTAAT TTTACGGGAA ATAATTCAAC GCCTGGTGGC 
GGCTCTGTAT TTAATATGAA CGGCAACACT AATGCTAATA CGGTGTTTGC CGGCTCTAAT 
AACCAACCAC ATCAATCGCA AACCCCATCT TTCAATACAA ACAGCTCATT CACGCCATCA 
ACAGTTCCTA ATATTAATTT TAGCGGATTG AATGGCGGAA TTACTAATAC CGCGACCAAT 
GCATTAAGGC CAAGTGATAT ATTTGGTGCG AATGCTGCCT CTGGTTCCAA TTCAAACGTA 
ACAAATCCAT CATCCATTTT TGGGGGGGCA GGTGGTGTGC CGACAACTTC TTTTGGGCAG 
CCGCAGTCAG CCCCTAATCA GATGGGGATG GGAACAAATA ATGGCATGAG CATGGGCGGT 
GGTGTTATGG CGAACAGAAA GATTGCAAGA ATGAGGCACT CTAAAAGGTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_014741.1
CDS	1..3231
Translation	MSSNTSSVMSSPRVEKRSFSSTLKSFFTNPNKKRPSSKKVFSSNLSYANHLEESDVEDTLHVNKRKRVSGTSQHSDSLTQNNNNAPIIIYGTENTERPPLLPILPIQRLRLLREKQRVRNMRELGLIQSTEFPSITSSVILGSQSKSDEGGSYLCTSSTPSPIKNGSCTRQLAGKSGEDTNVGLPILKSLKNRSNRKRFHSQSKGTVWSANFEYDLSEYDAIQKKDNKDKEGNAGGDQKTSENRNNIKSSISNGNLATGPNLTSEIEDLRADINSNRLSNPQKNLLLKGPASTVAKTAPIQESFVPNSERSGTPTLKKNIEPKKDKESIVLPTVGFDFIKDNETPSKKTSPKATSSAGAVFKSSVEMGKTDKSTKTAEAPTLSFNFSQKANKTKAVDNTVPSTTLFNFGGKSDTVTSASQPFKFGKTSEKSENHTESDAPPKSTAPIFSFGKQEENGDEGDDENEPKRKRRLPVSEDTNTKPLFDFGKTGDQKETKKGESEKDASGKPSFVFGASDKQAEGTPLFTFGKKADVTSNIDSSAQFTFGKAATAKETHTKPSETPATIVKKPTFTFGQSTSENKISEGSAKPTFSFSKSEEERKSSPISNEAAKPSFSFPGKPVDVQAPTDDKTLKPTFSFTEPAQKDSSVVSEPKKPSFTFASSKTSQPKPLFSFGKSDAAKEPPGSNTSFSFTKPPANETDKRPTPPSFTFGGSTTNNTTTTSTKPSFSFGAPESMKSTASTAAANTEKLSNGFSFTKFNHNKEKSNSPTSFFDGSASSTPIPVLGKPTDATGNTTSKSAFSFGTANTNGTNASANSTSFSFNAPATGNGTTTTSNTSGTNIAGTFNVGKPDQSIASGNTNGAGSAFGFSSSGTAATGAASNQSSFNFGNNGAGGLNPFTSATSSTNANAGLFNKPPSTNAQNVNVPSAFNFTGNNSTPGGGSVFNMNGNTNANTVFAGSNNQPHQSQTPSFNTNSSFTPSTVPNINFSGLNGGITNTATNALRPSDIFGANAASGSNSNVTNPSSIFGGAGGVPTTSFGQPQSAPNQMGMGTNNGMSMGGGVMANRKIARMRHSKR

Target ORF information:

RefSeq Version	NM_001183517.1
Organism	Saccharomyces cerevisiae S288C
Definition	Saccharomyces cerevisiae S288C FG-nucleoporin NUP1 (NUP1), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001183517.1

ORF Insert Sequence:

ATGTCTTCAA ACACTTCTTC TGTGATGTCT TCTCCACGTG TCGAAAAGAG ATCGTTTTCT 
TCCACTTTAA AATCATTCTT CACAAACCCC AATAAAAAAC GGCCATCGAG CAAGAAAGTT 
TTCAGTTCAA ACCTCTCATA CGCGAATCAT TTGGAGGAAT CAGATGTTGA AGACACACTG 
CATGTTAATA AGAGAAAGAG GGTGTCCGGT ACATCACAGC ATAGCGACAG CTTAACTCAG 
AACAACAATA ATGCGCCAAT TATAATATAT GGAACCGAAA ACACTGAGAG ACCGCCGCTT 
TTGCCAATTT TGCCCATTCA AAGACTGAGG TTATTAAGGG AGAAGCAGAG GGTGAGAAAT 
ATGCGTGAGC TTGGATTAAT TCAATCAACT GAATTTCCAT CTATTACATC GTCGGTTATA 
TTGGGCTCTC AAAGTAAAAG CGATGAGGGA GGATCGTACC TATGCACATC ATCTACTCCT 
TCCCCTATTA AAAACGGTTC TTGCACTAGG CAGTTGGCCG GAAAAAGCGG TGAAGACACC 
AATGTTGGAC TACCCATTCT CAAATCATTG AAAAATAGAT CCAATAGAAA AAGGTTTCAT 
AGTCAGTCAA AGGGGACCGT GTGGTCAGCA AATTTTGAAT ATGATTTGTC AGAATATGAC 
GCTATACAAA AAAAGGATAA CAAGGATAAG GAAGGTAACG CTGGCGGTGA TCAGAAGACA 
AGCGAGAATA GAAATAATAT TAAGAGTAGT ATTTCAAATG GCAATCTGGC TACAGGCCCT 
AACCTGACAA GCGAAATTGA AGACCTACGT GCAGACATCA ACTCTAATAG GTTATCGAAT 
CCTCAAAAAA ATCTACTTTT AAAAGGACCA GCTTCCACAG TTGCAAAAAC TGCCCCTATT 
CAGGAGAGCT TTGTTCCCAA TTCAGAGCGC TCTGGTACGC CTACGTTAAA GAAAAATATT 
GAGCCCAAAA AGGACAAAGA AAGTATTGTT TTGCCCACCG TAGGTTTTGA CTTTATCAAG 
GACAATGAGA CTCCATCTAA GAAAACTTCT CCTAAGGCAA CTTCTTCTGC AGGTGCAGTC 
TTTAAATCGA GTGTAGAAAT GGGAAAAACC GATAAGTCAA CGAAAACTGC CGAGGCGCCT 
ACCTTATCAT TCAATTTTAG CCAAAAGGCT AATAAAACTA AGGCTGTCGA CAATACTGTC 
CCTTCCACAA CTTTATTCAA TTTTGGTGGT AAATCAGATA CCGTTACTTC TGCCAGTCAA 
CCTTTTAAAT TTGGAAAGAC ATCCGAAAAA AGTGAAAATC ATACAGAATC AGACGCGCCT 
CCAAAATCAA CTGCTCCAAT ATTTTCTTTT GGTAAACAAG AAGAGAATGG TGATGAAGGT 
GATGATGAAA ATGAGCCCAA AAGAAAAAGG CGTTTACCTG TTAGCGAGGA TACAAACACC 
AAGCCTTTAT TCGATTTCGG CAAGACCGGT GATCAAAAGG AGACCAAAAA GGGAGAGTCA 
GAAAAGGACG CATCAGGGAA ACCAAGCTTT GTCTTTGGTG CAAGTGATAA GCAAGCTGAA 
GGTACACCAT TATTTACATT CGGAAAAAAA GCTGATGTAA CAAGCAATAT TGACTCCTCT 
GCACAATTTA CCTTTGGTAA AGCCGCCACC GCGAAAGAAA CACACACCAA ACCATCTGAG 
ACACCTGCCA CAATAGTCAA GAAGCCTACT TTTACTTTTG GGCAGTCAAC AAGTGAAAAT 
AAGATCTCTG AGGGAAGTGC GAAACCTACA TTCTCTTTCT CTAAGTCAGA GGAGGAACGT 
AAGAGTAGTC CAATTTCAAA CGAAGCAGCT AAACCCTCGT TTTCGTTTCC GGGCAAGCCT 
GTTGATGTTC AAGCACCGAC GGATGATAAG ACTCTCAAGC CAACTTTTTC TTTTACTGAA 
CCTGCTCAAA AAGATTCATC TGTTGTTTCG GAACCTAAAA AGCCCTCCTT TACGTTTGCG 
TCTTCAAAAA CCTCACAACC AAAGCCATTG TTTTCATTTG GTAAGTCAGA TGCAGCTAAA 
GAACCACCAG GCTCTAACAC CTCATTTTCT TTCACTAAAC CTCCTGCTAA TGAGACAGAT 
AAAAGACCTA CACCGCCATC TTTCACCTTT GGCGGTTCCA CAACAAATAA TACAACAACC 
ACTAGCACAA AACCATCTTT TAGTTTTGGG GCTCCCGAGT CGATGAAGTC GACAGCAAGT 
ACAGCGGCAG CAAATACGGA GAAGCTATCA AATGGCTTTT CCTTTACAAA GTTCAATCAC 
AATAAAGAAA AGTCAAACTC TCCAACTTCT TTCTTCGATG GTTCTGCTTC CTCAACGCCG 
ATTCCTGTCT TGGGTAAGCC AACAGACGCT ACTGGTAATA CAACATCTAA ATCTGCATTT 
TCATTCGGTA CTGCTAACAC CAATGGTACC AATGCCTCAG CAAACTCCAC ATCATTCTCG 
TTTAACGCCC CTGCTACTGG TAACGGCACA ACTACTACTT CCAATACCTC AGGAACCAAT 
ATAGCGGGTA CATTTAACGT AGGAAAACCG GATCAAAGTA TCGCTAGTGG CAATACGAAT 
GGAGCGGGCT CGGCATTTGG CTTTTCGAGC TCAGGAACAG CAGCAACTGG TGCAGCTTCT 
AATCAATCTT CATTTAATTT TGGAAACAAT GGTGCAGGGG GTCTCAATCC TTTTACATCA 
GCAACTTCGT CAACTAATGC TAATGCTGGT TTATTCAATA AACCTCCTTC CACGAATGCA 
CAAAATGTCA ATGTTCCCTC TGCTTTTAAT TTTACGGGAA ATAATTCAAC GCCTGGTGGC 
GGCTCTGTAT TTAATATGAA CGGCAACACT AATGCTAATA CGGTGTTTGC CGGCTCTAAT 
AACCAACCAC ATCAATCGCA AACCCCATCT TTCAATACAA ACAGCTCATT CACGCCATCA 
ACAGTTCCTA ATATTAATTT TAGCGGATTG AATGGCGGAA TTACTAATAC CGCGACCAAT 
GCATTAAGGC CAAGTGATAT ATTTGGTGCG AATGCTGCCT CTGGTTCCAA TTCAAACGTA 
ACAAATCCAT CATCCATTTT TGGGGGGGCA GGTGGTGTGC CGACAACTTC TTTTGGGCAG 
CCGCAGTCAG CCCCTAATCA GATGGGGATG GGAACAAATA ATGGCATGAG CATGGGCGGT 
GGTGTTATGG CGAACAGAAA GATTGCAAGA ATGAGGCACT CTAAAAGGTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The nucleotide sequence of Saccharomyces cerevisiae chromosome XV.
Nature387(6632 Suppl)98-102(1997 May)
Dujon B,Albermann K,Aldea M,Alexandraki D,Ansorge W,Arino J,Benes V,Bohn C,Bolotin-Fukuhara M,Bordonn? R,Boyer J,Camasses A,Casamayor A,Casas C,Ch?ret G,Cziepluch C,Daignan-Fornier B,Dang DV,de Haan M,Delius H,Durand P,Fairhead C,Feldmann H,Gaillon L,Kleine K

Life with 6000 genes.
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)
Goffeau A,Barrell BG,Bussey H,Davis RW,Dujon B,Feldmann H,Galibert F,Hoheisel JD,Jacq C,Johnston M,Louis EJ,Mewes HW,Murakami Y,Philippsen P,Tettelin H,Oliver SG

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NUP1 cDNA ORF clone, Saccharomyces cerevisiae S288C

Gene

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gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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