RRP6 cDNA ORF clone, Saccharomyces cerevisiae S288C

Gene
Clones
gRNAs

Summary

Gene Symbol	RRP6
Entrez Gene ID	854162
Full Name	exosome nuclease subunit RRP6
Gene Type	protein-coding
Organism	Saccharomyces cerevisiae S288C

mRNA and Protein(s)

mRNA	Protein	Name
NM_001183420.1	NP_014643.1	exosome nuclease subunit RRP6

Pathways from BioSystems

Homologies

Saccharomyces cerevisiae (baker's yeast)	RRP6	NP_014643.1
Neurospora crassa	NCU02256	XP_959900.1
Magnaporthe oryzae (rice blast fungus)	MGG_00896	XP_003718068.1
Eremothecium gossypii	AGOS_ACL001C	NP_983403.2
Kluyveromyces lactis	KLLA0D01309g	XP_453127.1
Schizosaccharomyces pombe (fission yeast)	rrp6	NP_593004.2

Gene Ontology
Bibliography

Related articles in PubMed

The nucleotide sequence of Saccharomyces cerevisiae chromosome XV.
Dujon B, Albermann K, Aldea M, Alexandraki D, Ansorge W, Arino J, Benes V, Bohn C, Bolotin-Fukuhara M, Bordonn? R, Boyer J, Camasses A, Casamayor A, Casas C, Ch?ret G, Cziepluch C, Daignan-Fornier B, Dang DV, de Haan M, Delius H, Durand P, Fairhead C, Feldmann H, Gaillon L, Kleine K
Nature387(6632 Suppl)98-102(1997 May)

Life with 6000 genes.
Goffeau A, Barrell BG, Bussey H, Davis RW, Dujon B, Feldmann H, Galibert F, Hoheisel JD, Jacq C, Johnston M, Louis EJ, Mewes HW, Murakami Y, Philippsen P, Tettelin H, Oliver SG
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Data suggest that, in nuclear exosomes, karyopherins are bound to the Rrp6 complex; Srp1, Kap95, and Sxm1 appear to be the most important karyopherins for nuclear import of Rrp6. (Rrp6 = exosome nuclease subunit RRP6; Srp1 = karyopherin alpha kap60; Kap95 = karyopherin beta Kap95; Sxm1 = karyopherin Kap108)
Title: Identification of karyopherins involved in the nuclear import of RNA exosome subunit Rrp6 in

Rrp41 mutations blocked substrate passage through the channel to Rrp44 only for cytoplasmic mRNAs, supporting the predicted widening of the lumen in the Rrp6-associated.Many exosome substrates exhibited clear preferences for a specific path to Rrp44. Both threading and direct access routes involve the RNA helicase Mtr4Rrp41 mutations blocked substrate passage through the channel to Rrp44 only for cytoplasmic mRNAs, suppor
Title: Transcriptome-wide analysis of alternative routes for RNA substrates into the exosome complex.

3' phosphate RNA is not a substrate for Rrp6 but is readily degraded by Rrp44 in the nuclear exosome.
Title: Nuclear RNA Exosome at 3.1?? Reveals Substrate Specificities, RNA Paths, and Allosteric Inhibition of Rrp44/Dis3.

Rrp6: Integrated roles in nuclear RNA metabolism and transcription termination
Title: Rrp6: Integrated roles in nuclear RNA metabolism and transcription termination.

The data raise the intriguing possibility that Nab3 and Nrd1 can function independently to recruit Rrp6 to ncRNA targets, providing combinatorial flexibility in RNA processing.
Title: Nab3 facilitates the function of the TRAMP complex in RNA processing via recruitment of Rrp6 independent of Nrd1.

The following RRP6 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the RRP6 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OSi03316	NM_001183420.1 Latest version!	Saccharomyces cerevisiae S288C exosome nuclease subunit RRP6 (RRP6), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

RRP6 ( NM_001183420.1 ) cDNA ORF clone, Saccharomyces cerevisiae S288C -> NP_014643.1 Saccharomyces cerevisiae S288C exosome nuclease subunit RRP6 (RRP6), partial mRNA.

Price & Availability↑

CloneID	OSi03316
Clone ID Related Accession (Same CDS sequence)	NM_001183420.1
Accession Version	NM_001183420.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2202bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-31
Organism	Saccharomyces cerevisiae S288C
Product	exosome nuclease subunit RRP6
Comment	Comment: REVIEWED REFSEQ: This record has been curated by SGD. This record is derived from an annotated genomic sequence (NC_001147). ##Genome-Annotation-Data-START## Annotation Provider :: SGD Annotation Status :: Full Annotation Annotation Version :: R64-2-1 URL :: http://www.yeastgenome.org/ ##Genome-Annotation-Data-END## COMPLETENESS: incomplete on both ends.

ATGACTTCTG AAAATCCGGA TGTACTTTTA TCTAGGGTGA TAAATGTGGT GAGAGCAGCA 
TCATCGTTAG CCAGTCAAGA TGTAGACTTT TACAAGAATT TAGACAGGGG ATTTAGTAAA 
GATCTGAAAT CAAAAGCAGA CAAACTTGCG GATATGGCGA ATGAAATAAT CCTCTCTATT 
GATGAGCATC ATGAATCATT TGAACTTAAA GAAGAGGACA TTTCTGACTT GTGGAACAAC 
TTTGGAAACA TCATGGATAA TTTGTTAGAA ATGTCAGACC ATTCGCTGGA TAAGCTGAAT 
TGTGCAATAA ACTCAAAGTC AAGAGGTTCC GATCTTCAGT ATTTAGGCGA ATTTTCAGGA 
AAGAATTTTT CTCCCACCAA AAGAGTCGAA AAACCTCAGT TGAAATTTAA AAGTCCAATT 
GATAACAGCG AAAGTCATCC GTTCATACCT TTATTGAAGG AAAAACCAAA TGCTTTGAAG 
CCACTCTCTG AGAGTTTGAG GTTGGTTGAT GATGATGAAA ATAACCCCAG TCACTACCCC 
CATCCTTATG AATATGAGAT TGATCATCAA GAGTATAGTC CAGAAATTCT CCAAATTAGA 
GAGGAGATTC CCTCAAAATC CTGGGATGAT TCGGTACCAA TTTGGGTAGA TACAAGTACA 
GAACTCGAAT CAATGTTGGA GGACTTGAAA AATACGAAAG AGATTGCCGT TGATCTTGAG 
CATCACGATT ATAGGTCATA CTATGGTATT GTTTGCCTCA TGCAAATTAG TACAAGGGAA 
AGGGATTATT TAGTTGATAC TCTAAAACTT CGCGAAAATT TACATATTCT GAACGAAGTG 
TTTACGAACC CTTCAATTGT GAAAGTTTTT CACGGTGCAT TCATGGATAT TATTTGGCTT 
CAGCGAGATT TAGGTTTGTA TGTCGTTGGT CTCTTCGATA CTTACCATGC GTCTAAAGCA 
ATCGGTCTTC CTAGACATAG TTTGGCCTAT CTTTTGGAGA ATTTTGCCAA TTTTAAAACT 
TCAAAGAAAT ATCAGTTGGC TGACTGGCGT ATAAGACCGC TTTCAAAACC CATGACGGCA 
TATGCTAGAG CAGATACTCA CTTTTTACTA AATATATATG ACCAGTTGAG AAACAAACTT 
ATCGAGTCTA ATAAGTTAGC AGGTGTTCTT TATGAATCAA GAAATGTCGC AAAAAGAAGG 
TTTGAATATT CAAAGTACAG ACCACTGACA CCTTCTTCAG AAGTTTATTC TCCAATCGAA 
AAAGAGAGTC CTTGGAAAAT ATTGATGTAT CAGTACAACA TACCACCTGA GAGGGAAGTA 
TTAGTAAGAG AGCTATACCA ATGGAGAGAT CTTATTGCAA GAAGAGACGA TGAATCGCCA 
CGATTTGTTA TGCCAAACCA GTTATTGGCT GCACTTGTCG CATATACACC TACTGATGTC 
ATAGGTGTTG TTTCTTTGAC CAATGGCGTA ACTGAACATG TCCGCCAGAA TGCAAAACTA 
TTAGCTAATT TGATAAGAGA TGCATTACGA AACATCAAGA ACACCAATGA GGAAGCTACT 
CCCATTCCAT CTTCCGAGAC CAAAGCAGAC GGAATATTGT TGGAGACTAT AAGTGTTCCT 
CAAATCCGTG ATGTTATGGA AAGGTTTTCG GTGTTGTGCA ACAGCAATAT TTCCAAGAGC 
CGAGCCAAGC CTGTCACTAA TTCTTCTATT CTGTTGGGTA AGATTTTACC ACGTGAGGAG 
CATGATATTG CATATAGCAA AGACGGCTTG CCAAATAAAG TGAAGACGGA AGATATCAGG 
ATCCGAGCAC AAAATTTCAA GTCTGCCTTA GCAAACTTGG AGGATATCAT ATTCGAAATA 
GAGAAACCTC TCGTTGTTCC TGTCAAGCTA GAGGAGATTA AGACAGTAGA CCCTGCGAGC 
GCACCTAATC ACTCGCCTGA GATTGATAAT CTTGATGATC TTGTTGTTTT AAAGAAGAAA 
AACATTCAAA AGAAACAACC AGCAAAGGAA AAAGGTGTAA CGGAAAAAGA TGCTGTGGAT 
TATTCAAAAA TTCCGAATAT CTTATCTAAT AAGCCTGGCC AAAATAATAG ACAACAAAAG 
AAAAGGAGAT TCGACCCATC ATCTAGCGAT AGTAATGGAC CAAGGGCAGC TAAAAAGAGG 
AGGCCTGCCG CCAAAGGTAA GAATCTGTCA TTTAAAAGGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_014643.1
CDS	1..2202
Translation	MTSENPDVLLSRVINVVRAASSLASQDVDFYKNLDRGFSKDLKSKADKLADMANEIILSIDEHHESFELKEEDISDLWNNFGNIMDNLLEMSDHSLDKLNCAINSKSRGSDLQYLGEFSGKNFSPTKRVEKPQLKFKSPIDNSESHPFIPLLKEKPNALKPLSESLRLVDDDENNPSHYPHPYEYEIDHQEYSPEILQIREEIPSKSWDDSVPIWVDTSTELESMLEDLKNTKEIAVDLEHHDYRSYYGIVCLMQISTRERDYLVDTLKLRENLHILNEVFTNPSIVKVFHGAFMDIIWLQRDLGLYVVGLFDTYHASKAIGLPRHSLAYLLENFANFKTSKKYQLADWRIRPLSKPMTAYARADTHFLLNIYDQLRNKLIESNKLAGVLYESRNVAKRRFEYSKYRPLTPSSEVYSPIEKESPWKILMYQYNIPPEREVLVRELYQWRDLIARRDDESPRFVMPNQLLAALVAYTPTDVIGVVSLTNGVTEHVRQNAKLLANLIRDALRNIKNTNEEATPIPSSETKADGILLETISVPQIRDVMERFSVLCNSNISKSRAKPVTNSSILLGKILPREEHDIAYSKDGLPNKVKTEDIRIRAQNFKSALANLEDIIFEIEKPLVVPVKLEEIKTVDPASAPNHSPEIDNLDDLVVLKKKNIQKKQPAKEKGVTEKDAVDYSKIPNILSNKPGQNNRQQKKRRFDPSSSDSNGPRAAKKRRPAAKGKNLSFKR

Target ORF information:

RefSeq Version	NM_001183420.1
Organism	Saccharomyces cerevisiae S288C
Definition	Saccharomyces cerevisiae S288C exosome nuclease subunit RRP6 (RRP6), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001183420.1

ORF Insert Sequence:

ATGACTTCTG AAAATCCGGA TGTACTTTTA TCTAGGGTGA TAAATGTGGT GAGAGCAGCA 
TCATCGTTAG CCAGTCAAGA TGTAGACTTT TACAAGAATT TAGACAGGGG ATTTAGTAAA 
GATCTGAAAT CAAAAGCAGA CAAACTTGCG GATATGGCGA ATGAAATAAT CCTCTCTATT 
GATGAGCATC ATGAATCATT TGAACTTAAA GAAGAGGACA TTTCTGACTT GTGGAACAAC 
TTTGGAAACA TCATGGATAA TTTGTTAGAA ATGTCAGACC ATTCGCTGGA TAAGCTGAAT 
TGTGCAATAA ACTCAAAGTC AAGAGGTTCC GATCTTCAGT ATTTAGGCGA ATTTTCAGGA 
AAGAATTTTT CTCCCACCAA AAGAGTCGAA AAACCTCAGT TGAAATTTAA AAGTCCAATT 
GATAACAGCG AAAGTCATCC GTTCATACCT TTATTGAAGG AAAAACCAAA TGCTTTGAAG 
CCACTCTCTG AGAGTTTGAG GTTGGTTGAT GATGATGAAA ATAACCCCAG TCACTACCCC 
CATCCTTATG AATATGAGAT TGATCATCAA GAGTATAGTC CAGAAATTCT CCAAATTAGA 
GAGGAGATTC CCTCAAAATC CTGGGATGAT TCGGTACCAA TTTGGGTAGA TACAAGTACA 
GAACTCGAAT CAATGTTGGA GGACTTGAAA AATACGAAAG AGATTGCCGT TGATCTTGAG 
CATCACGATT ATAGGTCATA CTATGGTATT GTTTGCCTCA TGCAAATTAG TACAAGGGAA 
AGGGATTATT TAGTTGATAC TCTAAAACTT CGCGAAAATT TACATATTCT GAACGAAGTG 
TTTACGAACC CTTCAATTGT GAAAGTTTTT CACGGTGCAT TCATGGATAT TATTTGGCTT 
CAGCGAGATT TAGGTTTGTA TGTCGTTGGT CTCTTCGATA CTTACCATGC GTCTAAAGCA 
ATCGGTCTTC CTAGACATAG TTTGGCCTAT CTTTTGGAGA ATTTTGCCAA TTTTAAAACT 
TCAAAGAAAT ATCAGTTGGC TGACTGGCGT ATAAGACCGC TTTCAAAACC CATGACGGCA 
TATGCTAGAG CAGATACTCA CTTTTTACTA AATATATATG ACCAGTTGAG AAACAAACTT 
ATCGAGTCTA ATAAGTTAGC AGGTGTTCTT TATGAATCAA GAAATGTCGC AAAAAGAAGG 
TTTGAATATT CAAAGTACAG ACCACTGACA CCTTCTTCAG AAGTTTATTC TCCAATCGAA 
AAAGAGAGTC CTTGGAAAAT ATTGATGTAT CAGTACAACA TACCACCTGA GAGGGAAGTA 
TTAGTAAGAG AGCTATACCA ATGGAGAGAT CTTATTGCAA GAAGAGACGA TGAATCGCCA 
CGATTTGTTA TGCCAAACCA GTTATTGGCT GCACTTGTCG CATATACACC TACTGATGTC 
ATAGGTGTTG TTTCTTTGAC CAATGGCGTA ACTGAACATG TCCGCCAGAA TGCAAAACTA 
TTAGCTAATT TGATAAGAGA TGCATTACGA AACATCAAGA ACACCAATGA GGAAGCTACT 
CCCATTCCAT CTTCCGAGAC CAAAGCAGAC GGAATATTGT TGGAGACTAT AAGTGTTCCT 
CAAATCCGTG ATGTTATGGA AAGGTTTTCG GTGTTGTGCA ACAGCAATAT TTCCAAGAGC 
CGAGCCAAGC CTGTCACTAA TTCTTCTATT CTGTTGGGTA AGATTTTACC ACGTGAGGAG 
CATGATATTG CATATAGCAA AGACGGCTTG CCAAATAAAG TGAAGACGGA AGATATCAGG 
ATCCGAGCAC AAAATTTCAA GTCTGCCTTA GCAAACTTGG AGGATATCAT ATTCGAAATA 
GAGAAACCTC TCGTTGTTCC TGTCAAGCTA GAGGAGATTA AGACAGTAGA CCCTGCGAGC 
GCACCTAATC ACTCGCCTGA GATTGATAAT CTTGATGATC TTGTTGTTTT AAAGAAGAAA 
AACATTCAAA AGAAACAACC AGCAAAGGAA AAAGGTGTAA CGGAAAAAGA TGCTGTGGAT 
TATTCAAAAA TTCCGAATAT CTTATCTAAT AAGCCTGGCC AAAATAATAG ACAACAAAAG 
AAAAGGAGAT TCGACCCATC ATCTAGCGAT AGTAATGGAC CAAGGGCAGC TAAAAAGAGG 
AGGCCTGCCG CCAAAGGTAA GAATCTGTCA TTTAAAAGGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The nucleotide sequence of Saccharomyces cerevisiae chromosome XV.
Nature387(6632 Suppl)98-102(1997 May)
Dujon B,Albermann K,Aldea M,Alexandraki D,Ansorge W,Arino J,Benes V,Bohn C,Bolotin-Fukuhara M,Bordonn? R,Boyer J,Camasses A,Casamayor A,Casas C,Ch?ret G,Cziepluch C,Daignan-Fornier B,Dang DV,de Haan M,Delius H,Durand P,Fairhead C,Feldmann H,Gaillon L,Kleine K

Life with 6000 genes.
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)
Goffeau A,Barrell BG,Bussey H,Davis RW,Dujon B,Feldmann H,Galibert F,Hoheisel JD,Jacq C,Johnston M,Louis EJ,Mewes HW,Murakami Y,Philippsen P,Tettelin H,Oliver SG

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RRP6 cDNA ORF clone, Saccharomyces cerevisiae S288C

Gene

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gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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