SIN3 cDNA ORF clone, Saccharomyces cerevisiae S288C

Gene
Clones
gRNAs

Summary

Gene Symbol	SIN3
Entrez Gene ID	854158
Full Name	transcriptional regulator SIN3
Synonyms	CPE1,GAM2,RPD1,SDI1,SDS16,UME4
Gene Type	protein-coding
Organism	Saccharomyces cerevisiae S288C

mRNA and Protein(s)

mRNA	Protein	Name
NM_001183258.1	NP_014639.1	transcriptional regulator SIN3

Pathways from BioSystems

Homologies

Saccharomyces cerevisiae (baker's yeast)	SIN3	NP_014639.1
Eremothecium gossypii	AGOS_ACL004W	NP_983400.1
Schizosaccharomyces pombe (fission yeast)	pst1	NP_596012.2
Danio rerio (zebrafish)	sin3ab	XP_002662923.1
Kluyveromyces lactis	KLLA0C06182g	XP_452473.1
Neurospora crassa	NCU02578	XP_965709.2
Danio rerio (zebrafish)	sin3aa	NP_001091650.1
Magnaporthe oryzae (rice blast fungus)	MGG_13498	XP_003716276.1

Gene Ontology
Bibliography

Related articles in PubMed

The nucleotide sequence of Saccharomyces cerevisiae chromosome XV.
Dujon B, Albermann K, Aldea M, Alexandraki D, Ansorge W, Arino J, Benes V, Bohn C, Bolotin-Fukuhara M, Bordonn? R, Boyer J, Camasses A, Casamayor A, Casas C, Ch?ret G, Cziepluch C, Daignan-Fornier B, Dang DV, de Haan M, Delius H, Durand P, Fairhead C, Feldmann H, Gaillon L, Kleine K
Nature387(6632 Suppl)98-102(1997 May)

Life with 6000 genes.
Goffeau A, Barrell BG, Bussey H, Davis RW, Dujon B, Feldmann H, Galibert F, Hoheisel JD, Jacq C, Johnston M, Louis EJ, Mewes HW, Murakami Y, Philippsen P, Tettelin H, Oliver SG
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

We also present evidence that indicates Rim11/Ume6 works independently but in parallel with that of checkpoint inhibition, dNTP regulation, and sister-chromatid recombination. In conclusion, our results establish Rim11, Ume6, the histone deacetylase complex Sin3-Rpd3 and Sml1 as new factors important in the events of faulty lagging strand synthesis.
Title: GSK-3? Homolog Rim11 and the Histone Deacetylase Complex Ume6-Sin3-Rpd3 Are Involved in Replication Stress Response Caused by Defects in Dna2.

Based on findings, authors conclude that the Tor and PpSin3-PpRpd3 pathway regulates PpAtg32 expression, but not PpAtg32 phosphorylation.
Title: Tor and the Sin3-Rpd3 complex regulate expression of the mitophagy receptor protein Atg32 in yeast.

These results show that the sin3 mutation decreases both spontaneous and UV-induced levels of levels.
Title: [Control levels of Sin3 histone deacetylase for spontaneous and UV-induced mutagenesis in yeasts Saccharomyces cerevisiae].

Ume6 is a negative regulator of ATG8 transcription, which acts along with a histone deacetylase complex including Sin3 and Rpd3 to regulate Atg8 levels
Title: Ume6 transcription factor is part of a signaling cascade that regulates autophagy.

investigated the regulatory contribution of subunits of Sin3 complexes and identified Pho23 as important for inositol and choline-dependent gene repression
Title: Multiple histone deacetylases are recruited by corepressor Sin3 and contribute to gene repression mediated by Opi1 regulator of phospholipid biosynthesis in the yeast Saccharomyces cerevisiae.

The following SIN3 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the SIN3 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OSi03312	NM_001183258.1 Latest version!	Saccharomyces cerevisiae S288C transcriptional regulator SIN3 (SIN3), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

SIN3 ( NM_001183258.1 ) cDNA ORF clone, Saccharomyces cerevisiae S288C -> NP_014639.1 Saccharomyces cerevisiae S288C transcriptional regulator SIN3 (SIN3), partial mRNA.

Price & Availability↑

CloneID	OSi03312
Clone ID Related Accession (Same CDS sequence)	NM_001183258.1
Accession Version	NM_001183258.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4611bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-31
Organism	Saccharomyces cerevisiae S288C
Product	transcriptional regulator SIN3
Comment	Comment: REVIEWED REFSEQ: This record has been curated by SGD. This record is derived from an annotated genomic sequence (NC_001147). ##Genome-Annotation-Data-START## Annotation Provider :: SGD Annotation Status :: Full Annotation Annotation Version :: R64-2-1 URL :: http://www.yeastgenome.org/ ##Genome-Annotation-Data-END## COMPLETENESS: incomplete on both ends.

ATGTCACAGG TTTGGCATAA TTCGAATTCG CAATCAAACG ATGTGGCTAC TTCAAATGAC 
GCTACGGGTT CCAACGAAAG AAACGAAAAA GAACCGTCCC TCCAGGGAAA TAAGCCCGGT 
TTTGTTCAAC AGCAGCAACG GATTACTTTA CCCTCGCTAT CTGCCTTGAG TACTAAGGAG 
GAAGATAGAA GAGATTCCAA TGGCCAACAG GCTCTAACTT CTCATGCTGC TCACATATTA 
GGTTATCCTC CCCCACATTC AAATGCTATG CCCTCAATTG CAACTGATTC AGCATTGAAA 
CAGCCCCACG AGTATCACCC TCGCCCTAAA TCTTCGTCCT CTTCTCCCTC TATAAACGCT 
TCGCTTATGA ATGCTGGTCC AGCTCCCCTC CCCACAGTGG GAGCCGCCAG TTTTTCTTTG 
TCGAGATTTG ACAATCCATT ACCGATAAAA GCTCCTGTTC ATACAGAGGA ACCAAAAAGT 
TATAATGGTC TTCAGGAAGA AGAAAAGGCG ACGCAACGGC CTCAAGATTG CAAGGAAGTT 
CCCGCTGGTG TGCAGCCTGC TGATGCCCCT GACCCTAGCA GCAACCATGC AGATGCTAAC 
GATGACAATA ATAACAACGA AAATTCTCAC GATGAAGATG CTGACTACAG ACCTCTAAAC 
GTGAAGGATG CCCTATCTTA CCTCGAACAG GTCAAATTTC AATTTAGTTC GCGTCCGGAT 
ATCTATAATT TATTTTTAGA TATTATGAAG GACTTTAAAT CTCAGGCAAT AGACACACCG 
GGCGTTATTG AAAGAGTATC CACTTTGTTC AGAGGTTATC CAATTTTGAT TCAAGGGTTC 
AATACTTTTC TACCCCAAGG CTATAGAATC GAATGCTCCT CTAATCCGGA CGACCCCATT 
AGAGTTACTA CACCAATGGG TACTACGACA GTAAACAATA ACATCAGTCC ATCTGGTAGA 
GGTACAACGG ATGCACAGGA ACTTGGTTCT TTTCCAGAAA GCGATGGAAA TGGTGTTCAA 
CAGCCCTCCA ATGTGCCAAT GGTGCCTTCG AGTGTGTATC AATCGGAACA AAACCAAGAC 
CAACAACAAT CTTTGCCTCT TTTAGCTACT TCTTCTGGTT TACCTTCAAT TCAACAACCT 
GAAATGCCTG CACATCGCCA AATCCCACAA AGTCAATCTT TAGTGCCTCA AGAAGATGCT 
AAGAAAAACG TTGATGTCGA ATTTAGTCAA GCCATAAGCT ACGTTAATAA AATTAAAACT 
AGATTTGCCG ACCAACCTGA TATTTACAAG CATTTTCTGG AAATACTACA AACTTATCAG 
CGAGAGCAAA AGCCAATAAA CGAAGTCTAC GCACAAGTGA CGCATCTTTT CCAAAATGCT 
CCTGATTTAC TAGAAGATTT CAAGAAATTC TTGCCGGACT CTTCAGCTTC TGCCAATCAG 
CAGGTGCAAC ATGCTCAGCA ACATGCTCAA CAACAACATG AGGCCCAAAT GCATGCACAG 
GCACAAGCTC AGGCTCAGGC ACAGGCACAG GTGGAACAAC AGAAGCAGCA ACAGCAATTC 
TTGTATCCAG CTTCGGGCTA TTACGGCCAT CCTTCTAACC GAGGTATTCC ACAGCAAAAC 
TTGCCTCCTA TAGGAAGCTT CTCGCCTCCA ACAAACGGTT CGACCGTACA TGAAGCCTAT 
CAGGATCAAC AGCACATGCA ACCACCCCAC TTCATGCCCT TACCATCGAT CGTTCAACAC 
GGGCCAAATA TGGTACATCA AGGGATAGCG AATGAAAATC CACCTCTATC AGATCTAAGA 
ACGTCTCTCA CTGAACAGTA CGCTCCTTCT AGTATACAAC ATCAGCAGCA GCACCCGCAA 
AGTATTAGTC CTATAGCAAA TACGCAGTAT GGTGATATCC CTGTTAGACC GGAAATTGAT 
TTAGATCCTA GCATTGTGCC TGTGGTCCCT GAACCCACTG AGCCCATCGA AAACAATATA 
TCGCTTAATG AGGAAGTCAC TTTCTTCGAA AAGGCCAAGA GGTATATCGG CAATAAACAT 
TTATACACTG AGTTTTTGAA AATTTTAAAT TTGTACTCTC AAGATATACT TGATCTTGAC 
GATTTAGTGG AAAAGGTAGA TTTCTACTTG GGTTCCAATA AAGAACTATT TACGTGGTTC 
AAAAACTTTG TTGGCTACCA AGAAAAAACC AAATGTATCG AGAATATTGT TCATGAAAAA 
CATAGATTGG ATTTAGATTT ATGTGAGGCA TTTGGCCCAA GTTACAAGAG GCTACCAAAA 
AGTGACACTT TCATGCCATG CTCAGGTAGG GATGATATGT GTTGGGAAGT CTTGAACGAT 
GAATGGGTTG GACATCCTGT ATGGGCTTCC GAAGATTCGG GATTTATTGC TCATCGTAAA 
AACCAGTATG AGGAAACACT ATTCAAGATC GAAGAGGAAA GACATGAGTA TGATTTTTAC 
ATTGAATCAA ATTTAAGAAC TATTCAATGT TTGGAAACAA TTGTAAATAA GATCGAGAAC 
ATGACTGAGA ATGAAAAAGC CAATTTTAAA CTGCCTCCAG GTCTTGGCCA TACTTCAATG 
ACTATTTATA AAAAAGTGAT AAGGAAAGTT TATGATAAGG AAAGGGGGTT CGAGATTATT 
GATGCTTTGC ATGAGCACCC TGCAGTGACA GCCCCAGTCG TTCTGAAAAG GTTAAAGCAA 
AAAGACGAAG AATGGAGAAG AGCTCAACGT GAATGGAATA AAGTTTGGAG GGAGTTAGAA 
CAGAAGGTTT TTTTCAAGTC ATTAGATCAT TTAGGCTTAA CATTTAAACA GGCTGACAAG 
AAATTATTAA CTACAAAGCA GTTGATATCA GAGATTAGCA GCATCAAAGT TGATCAAACA 
AACAAAAAAA TTCACTGGTT AACTCCTAAA CCAAAGAGCC AGTTAGATTT TGATTTCCCT 
GATAAAAACA TTTTCTATGA TATCTTGTGT TTGGCTGACA CTTTTATAAC CCATACCACA 
GCCTATTCTA ATCCCGATAA AGAAAGATTG AAAGATTTAC TAAAATACTT CATCTCTTTG 
TTTTTTTCTA TTTCTTTCGA AAAAATCGAA GAATCGTTGT ACTCCCATAA GCAAAACGTG 
TCAGAATCTA GCGGATCTGA CGATGGCAGT TCTATTGCAT CAAGGAAGAG GCCCTATCAA 
CAAGAAATGA GTTTACTTGA TATTTTACAC AGGAGCAGAT ATCAAAAGCT AAAGCGTTCT 
AATGATGAAG ATGGCAAAGT TCCCCAGCTC TCTGAACCAC CCGAAGAAGA ACCTAATACC 
ATTGAGGAGG AAGAGCTCAT CGATGAAGAA GCTAAAAATC CGTGGCTAAC TGGGAATTTA 
GTGGAGGAAG CAAACTCTCA GGGTATTATC CAAAATCGCA GTATTTTTAA TCTATTCGCT 
AATACGAATA TTTACATATT TTTCCGTCAT TGGACAACGA TTTATGAGCG GCTTTTGGAA 
ATTAAGCAAA TGAATGAAAG GGTCACAAAG GAAATCAACA CAAGGTCGAC AGTTACTTTT 
GCCAAAGATC TAGATTTATT ATCGAGTCAA CTTTCCGAAA TGGGACTAGA TTTTGTTGGT 
GAAGACGCGT ACAAACAGGT TTTAAGACTG AGTAGAAGGT TGATTAATGG GGATCTTGAA 
CATCAGTGGT TTGAAGAGAG TTTGCGGCAA GCTTACAATA ACAAAGCGTT TAAACTCTAT 
ACAATTGATA AAGTCACCCA ATCGTTGGTA AAGCATGCTC ATACCTTGAT GACTGACGCT 
AAAACTGCGG AAATAATGGC TTTGTTCGTT AAAGATAGAA ATGCCTCCAC CACGAGTGCG 
AAGGACCAAA TTATCTATCG CTTGCAGGTG CGCTCACATA TGTCCAACAC AGAAAATATG 
TTTAGAATAG AGTTTGATAA AAGAACTCTG CATGTTTCCA TTCAATATAT TGCACTTGAT 
GATTTGACAC TAAAGGAACC AAAGGCAGAC GAAGATAAAT GGAAATATTA TGTAACATCG 
TATGCTCTGC CACATCCAAC AGAAGGCATT CCGCACGAGA AACTGAAGAT ACCATTTTTG 
GAAAGGCTCA TCGAATTTGG ACAAGATATT GATGGAACAG AGGTAGATGA GGAGTTTTCC 
CCCGAAGGCA TCTCTGTATC GACATTGAAA ATTAAGATCC AGCCTATAAC CTACCAACTG 
CATATTGAAA ACGGGTCTTA CGATGTTTTT ACCCGTAAGG CTACTAACAA ATATCCTACT 
ATTGCTAATG ATAATACTCA AAAAGGAATG GTTTCTCAGA AGAAGGAGTT GATATCAAAA 
TTTCTAGACT GTGCAGTTGG TTTGAGAAAT AATTTAGATG AGGCTCAAAA ATTAAGTATG 
CAAAAAAAAT GGGAGAACCT AAAAGACAGC ATAGCAAAGA CGAGTGCTGG AAACCAGGGA 
ATAGAGAGTG AAACCGAAAA AGGTAAAATT ACGAAACAGG AGCAGTCGGA TAATTTGGAC 
AGCTCTACTG CAAGTGTACT GCCTGCATCC ATTACTACTG TACCACAAGA TGATAATATA 
GAAACGACTG GGAATACTGA ATCTTCAGAC AAGGGGGCTA AGATTCAATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_014639.1
CDS	1..4611
Translation	MSQVWHNSNSQSNDVATSNDATGSNERNEKEPSLQGNKPGFVQQQQRITLPSLSALSTKEEDRRDSNGQQALTSHAAHILGYPPPHSNAMPSIATDSALKQPHEYHPRPKSSSSSPSINASLMNAGPAPLPTVGAASFSLSRFDNPLPIKAPVHTEEPKSYNGLQEEEKATQRPQDCKEVPAGVQPADAPDPSSNHADANDDNNNNENSHDEDADYRPLNVKDALSYLEQVKFQFSSRPDIYNLFLDIMKDFKSQAIDTPGVIERVSTLFRGYPILIQGFNTFLPQGYRIECSSNPDDPIRVTTPMGTTTVNNNISPSGRGTTDAQELGSFPESDGNGVQQPSNVPMVPSSVYQSEQNQDQQQSLPLLATSSGLPSIQQPEMPAHRQIPQSQSLVPQEDAKKNVDVEFSQAISYVNKIKTRFADQPDIYKHFLEILQTYQREQKPINEVYAQVTHLFQNAPDLLEDFKKFLPDSSASANQQVQHAQQHAQQQHEAQMHAQAQAQAQAQAQVEQQKQQQQFLYPASGYYGHPSNRGIPQQNLPPIGSFSPPTNGSTVHEAYQDQQHMQPPHFMPLPSIVQHGPNMVHQGIANENPPLSDLRTSLTEQYAPSSIQHQQQHPQSISPIANTQYGDIPVRPEIDLDPSIVPVVPEPTEPIENNISLNEEVTFFEKAKRYIGNKHLYTEFLKILNLYSQDILDLDDLVEKVDFYLGSNKELFTWFKNFVGYQEKTKCIENIVHEKHRLDLDLCEAFGPSYKRLPKSDTFMPCSGRDDMCWEVLNDEWVGHPVWASEDSGFIAHRKNQYEETLFKIEEERHEYDFYIESNLRTIQCLETIVNKIENMTENEKANFKLPPGLGHTSMTIYKKVIRKVYDKERGFEIIDALHEHPAVTAPVVLKRLKQKDEEWRRAQREWNKVWRELEQKVFFKSLDHLGLTFKQADKKLLTTKQLISEISSIKVDQTNKKIHWLTPKPKSQLDFDFPDKNIFYDILCLADTFITHTTAYSNPDKERLKDLLKYFISLFFSISFEKIEESLYSHKQNVSESSGSDDGSSIASRKRPYQQEMSLLDILHRSRYQKLKRSNDEDGKVPQLSEPPEEEPNTIEEEELIDEEAKNPWLTGNLVEEANSQGIIQNRSIFNLFANTNIYIFFRHWTTIYERLLEIKQMNERVTKEINTRSTVTFAKDLDLLSSQLSEMGLDFVGEDAYKQVLRLSRRLINGDLEHQWFEESLRQAYNNKAFKLYTIDKVTQSLVKHAHTLMTDAKTAEIMALFVKDRNASTTSAKDQIIYRLQVRSHMSNTENMFRIEFDKRTLHVSIQYIALDDLTLKEPKADEDKWKYYVTSYALPHPTEGIPHEKLKIPFLERLIEFGQDIDGTEVDEEFSPEGISVSTLKIKIQPITYQLHIENGSYDVFTRKATNKYPTIANDNTQKGMVSQKKELISKFLDCAVGLRNNLDEAQKLSMQKKWENLKDSIAKTSAGNQGIESETEKGKITKQEQSDNLDSSTASVLPASITTVPQDDNIETTGNTESSDKGAKIQ

Target ORF information:

RefSeq Version	NM_001183258.1
Organism	Saccharomyces cerevisiae S288C
Definition	Saccharomyces cerevisiae S288C transcriptional regulator SIN3 (SIN3), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001183258.1

ORF Insert Sequence:

ATGTCACAGG TTTGGCATAA TTCGAATTCG CAATCAAACG ATGTGGCTAC TTCAAATGAC 
GCTACGGGTT CCAACGAAAG AAACGAAAAA GAACCGTCCC TCCAGGGAAA TAAGCCCGGT 
TTTGTTCAAC AGCAGCAACG GATTACTTTA CCCTCGCTAT CTGCCTTGAG TACTAAGGAG 
GAAGATAGAA GAGATTCCAA TGGCCAACAG GCTCTAACTT CTCATGCTGC TCACATATTA 
GGTTATCCTC CCCCACATTC AAATGCTATG CCCTCAATTG CAACTGATTC AGCATTGAAA 
CAGCCCCACG AGTATCACCC TCGCCCTAAA TCTTCGTCCT CTTCTCCCTC TATAAACGCT 
TCGCTTATGA ATGCTGGTCC AGCTCCCCTC CCCACAGTGG GAGCCGCCAG TTTTTCTTTG 
TCGAGATTTG ACAATCCATT ACCGATAAAA GCTCCTGTTC ATACAGAGGA ACCAAAAAGT 
TATAATGGTC TTCAGGAAGA AGAAAAGGCG ACGCAACGGC CTCAAGATTG CAAGGAAGTT 
CCCGCTGGTG TGCAGCCTGC TGATGCCCCT GACCCTAGCA GCAACCATGC AGATGCTAAC 
GATGACAATA ATAACAACGA AAATTCTCAC GATGAAGATG CTGACTACAG ACCTCTAAAC 
GTGAAGGATG CCCTATCTTA CCTCGAACAG GTCAAATTTC AATTTAGTTC GCGTCCGGAT 
ATCTATAATT TATTTTTAGA TATTATGAAG GACTTTAAAT CTCAGGCAAT AGACACACCG 
GGCGTTATTG AAAGAGTATC CACTTTGTTC AGAGGTTATC CAATTTTGAT TCAAGGGTTC 
AATACTTTTC TACCCCAAGG CTATAGAATC GAATGCTCCT CTAATCCGGA CGACCCCATT 
AGAGTTACTA CACCAATGGG TACTACGACA GTAAACAATA ACATCAGTCC ATCTGGTAGA 
GGTACAACGG ATGCACAGGA ACTTGGTTCT TTTCCAGAAA GCGATGGAAA TGGTGTTCAA 
CAGCCCTCCA ATGTGCCAAT GGTGCCTTCG AGTGTGTATC AATCGGAACA AAACCAAGAC 
CAACAACAAT CTTTGCCTCT TTTAGCTACT TCTTCTGGTT TACCTTCAAT TCAACAACCT 
GAAATGCCTG CACATCGCCA AATCCCACAA AGTCAATCTT TAGTGCCTCA AGAAGATGCT 
AAGAAAAACG TTGATGTCGA ATTTAGTCAA GCCATAAGCT ACGTTAATAA AATTAAAACT 
AGATTTGCCG ACCAACCTGA TATTTACAAG CATTTTCTGG AAATACTACA AACTTATCAG 
CGAGAGCAAA AGCCAATAAA CGAAGTCTAC GCACAAGTGA CGCATCTTTT CCAAAATGCT 
CCTGATTTAC TAGAAGATTT CAAGAAATTC TTGCCGGACT CTTCAGCTTC TGCCAATCAG 
CAGGTGCAAC ATGCTCAGCA ACATGCTCAA CAACAACATG AGGCCCAAAT GCATGCACAG 
GCACAAGCTC AGGCTCAGGC ACAGGCACAG GTGGAACAAC AGAAGCAGCA ACAGCAATTC 
TTGTATCCAG CTTCGGGCTA TTACGGCCAT CCTTCTAACC GAGGTATTCC ACAGCAAAAC 
TTGCCTCCTA TAGGAAGCTT CTCGCCTCCA ACAAACGGTT CGACCGTACA TGAAGCCTAT 
CAGGATCAAC AGCACATGCA ACCACCCCAC TTCATGCCCT TACCATCGAT CGTTCAACAC 
GGGCCAAATA TGGTACATCA AGGGATAGCG AATGAAAATC CACCTCTATC AGATCTAAGA 
ACGTCTCTCA CTGAACAGTA CGCTCCTTCT AGTATACAAC ATCAGCAGCA GCACCCGCAA 
AGTATTAGTC CTATAGCAAA TACGCAGTAT GGTGATATCC CTGTTAGACC GGAAATTGAT 
TTAGATCCTA GCATTGTGCC TGTGGTCCCT GAACCCACTG AGCCCATCGA AAACAATATA 
TCGCTTAATG AGGAAGTCAC TTTCTTCGAA AAGGCCAAGA GGTATATCGG CAATAAACAT 
TTATACACTG AGTTTTTGAA AATTTTAAAT TTGTACTCTC AAGATATACT TGATCTTGAC 
GATTTAGTGG AAAAGGTAGA TTTCTACTTG GGTTCCAATA AAGAACTATT TACGTGGTTC 
AAAAACTTTG TTGGCTACCA AGAAAAAACC AAATGTATCG AGAATATTGT TCATGAAAAA 
CATAGATTGG ATTTAGATTT ATGTGAGGCA TTTGGCCCAA GTTACAAGAG GCTACCAAAA 
AGTGACACTT TCATGCCATG CTCAGGTAGG GATGATATGT GTTGGGAAGT CTTGAACGAT 
GAATGGGTTG GACATCCTGT ATGGGCTTCC GAAGATTCGG GATTTATTGC TCATCGTAAA 
AACCAGTATG AGGAAACACT ATTCAAGATC GAAGAGGAAA GACATGAGTA TGATTTTTAC 
ATTGAATCAA ATTTAAGAAC TATTCAATGT TTGGAAACAA TTGTAAATAA GATCGAGAAC 
ATGACTGAGA ATGAAAAAGC CAATTTTAAA CTGCCTCCAG GTCTTGGCCA TACTTCAATG 
ACTATTTATA AAAAAGTGAT AAGGAAAGTT TATGATAAGG AAAGGGGGTT CGAGATTATT 
GATGCTTTGC ATGAGCACCC TGCAGTGACA GCCCCAGTCG TTCTGAAAAG GTTAAAGCAA 
AAAGACGAAG AATGGAGAAG AGCTCAACGT GAATGGAATA AAGTTTGGAG GGAGTTAGAA 
CAGAAGGTTT TTTTCAAGTC ATTAGATCAT TTAGGCTTAA CATTTAAACA GGCTGACAAG 
AAATTATTAA CTACAAAGCA GTTGATATCA GAGATTAGCA GCATCAAAGT TGATCAAACA 
AACAAAAAAA TTCACTGGTT AACTCCTAAA CCAAAGAGCC AGTTAGATTT TGATTTCCCT 
GATAAAAACA TTTTCTATGA TATCTTGTGT TTGGCTGACA CTTTTATAAC CCATACCACA 
GCCTATTCTA ATCCCGATAA AGAAAGATTG AAAGATTTAC TAAAATACTT CATCTCTTTG 
TTTTTTTCTA TTTCTTTCGA AAAAATCGAA GAATCGTTGT ACTCCCATAA GCAAAACGTG 
TCAGAATCTA GCGGATCTGA CGATGGCAGT TCTATTGCAT CAAGGAAGAG GCCCTATCAA 
CAAGAAATGA GTTTACTTGA TATTTTACAC AGGAGCAGAT ATCAAAAGCT AAAGCGTTCT 
AATGATGAAG ATGGCAAAGT TCCCCAGCTC TCTGAACCAC CCGAAGAAGA ACCTAATACC 
ATTGAGGAGG AAGAGCTCAT CGATGAAGAA GCTAAAAATC CGTGGCTAAC TGGGAATTTA 
GTGGAGGAAG CAAACTCTCA GGGTATTATC CAAAATCGCA GTATTTTTAA TCTATTCGCT 
AATACGAATA TTTACATATT TTTCCGTCAT TGGACAACGA TTTATGAGCG GCTTTTGGAA 
ATTAAGCAAA TGAATGAAAG GGTCACAAAG GAAATCAACA CAAGGTCGAC AGTTACTTTT 
GCCAAAGATC TAGATTTATT ATCGAGTCAA CTTTCCGAAA TGGGACTAGA TTTTGTTGGT 
GAAGACGCGT ACAAACAGGT TTTAAGACTG AGTAGAAGGT TGATTAATGG GGATCTTGAA 
CATCAGTGGT TTGAAGAGAG TTTGCGGCAA GCTTACAATA ACAAAGCGTT TAAACTCTAT 
ACAATTGATA AAGTCACCCA ATCGTTGGTA AAGCATGCTC ATACCTTGAT GACTGACGCT 
AAAACTGCGG AAATAATGGC TTTGTTCGTT AAAGATAGAA ATGCCTCCAC CACGAGTGCG 
AAGGACCAAA TTATCTATCG CTTGCAGGTG CGCTCACATA TGTCCAACAC AGAAAATATG 
TTTAGAATAG AGTTTGATAA AAGAACTCTG CATGTTTCCA TTCAATATAT TGCACTTGAT 
GATTTGACAC TAAAGGAACC AAAGGCAGAC GAAGATAAAT GGAAATATTA TGTAACATCG 
TATGCTCTGC CACATCCAAC AGAAGGCATT CCGCACGAGA AACTGAAGAT ACCATTTTTG 
GAAAGGCTCA TCGAATTTGG ACAAGATATT GATGGAACAG AGGTAGATGA GGAGTTTTCC 
CCCGAAGGCA TCTCTGTATC GACATTGAAA ATTAAGATCC AGCCTATAAC CTACCAACTG 
CATATTGAAA ACGGGTCTTA CGATGTTTTT ACCCGTAAGG CTACTAACAA ATATCCTACT 
ATTGCTAATG ATAATACTCA AAAAGGAATG GTTTCTCAGA AGAAGGAGTT GATATCAAAA 
TTTCTAGACT GTGCAGTTGG TTTGAGAAAT AATTTAGATG AGGCTCAAAA ATTAAGTATG 
CAAAAAAAAT GGGAGAACCT AAAAGACAGC ATAGCAAAGA CGAGTGCTGG AAACCAGGGA 
ATAGAGAGTG AAACCGAAAA AGGTAAAATT ACGAAACAGG AGCAGTCGGA TAATTTGGAC 
AGCTCTACTG CAAGTGTACT GCCTGCATCC ATTACTACTG TACCACAAGA TGATAATATA 
GAAACGACTG GGAATACTGA ATCTTCAGAC AAGGGGGCTA AGATTCAATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The nucleotide sequence of Saccharomyces cerevisiae chromosome XV.
Nature387(6632 Suppl)98-102(1997 May)
Dujon B,Albermann K,Aldea M,Alexandraki D,Ansorge W,Arino J,Benes V,Bohn C,Bolotin-Fukuhara M,Bordonn? R,Boyer J,Camasses A,Casamayor A,Casas C,Ch?ret G,Cziepluch C,Daignan-Fornier B,Dang DV,de Haan M,Delius H,Durand P,Fairhead C,Feldmann H,Gaillon L,Kleine K

Life with 6000 genes.
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)
Goffeau A,Barrell BG,Bussey H,Davis RW,Dujon B,Feldmann H,Galibert F,Hoheisel JD,Jacq C,Johnston M,Louis EJ,Mewes HW,Murakami Y,Philippsen P,Tettelin H,Oliver SG

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SIN3 cDNA ORF clone, Saccharomyces cerevisiae S288C

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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