PAP2 cDNA ORF clone, Saccharomyces cerevisiae S288C

Gene
Clones
gRNAs

Summary

Gene Symbol	PAP2
Entrez Gene ID	854034
Full Name	non-canonical poly(A) polymerase PAP2
Synonyms	TRF4
Gene Type	protein-coding
Organism	Saccharomyces cerevisiae S288C

mRNA and Protein(s)

mRNA	Protein	Name
NM_001183369.1	NP_014526.1	non-canonical poly(A) polymerase PAP2

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

The nucleotide sequence of Saccharomyces cerevisiae chromosome XV.
Dujon B, Albermann K, Aldea M, Alexandraki D, Ansorge W, Arino J, Benes V, Bohn C, Bolotin-Fukuhara M, Bordonn? R, Boyer J, Camasses A, Casamayor A, Casas C, Ch?ret G, Cziepluch C, Daignan-Fornier B, Dang DV, de Haan M, Delius H, Durand P, Fairhead C, Feldmann H, Gaillon L, Kleine K
Nature387(6632 Suppl)98-102(1997 May)

Life with 6000 genes.
Goffeau A, Barrell BG, Bussey H, Davis RW, Dujon B, Feldmann H, Galibert F, Hoheisel JD, Jacq C, Johnston M, Louis EJ, Mewes HW, Murakami Y, Philippsen P, Tettelin H, Oliver SG
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Results show that the Nrd1-Trf4 interaction is required for optimal exosome activity in vivo and for the stimulation of polyadenylation of NNS targets by TRAMP in vitro.
Title: Molecular basis for coordinating transcription termination with noncoding RNA degradation.

Cotranscriptional recruitment of RNA exosome cofactors Rrp47p and Mpp6p and two distinct Trf-Air-Mtr4 polyadenylation (TRAMP) complexes assists the exonuclease Rrp6p in the targeting and degradation of an aberrant messenger
Title: Cotranscriptional recruitment of RNA exosome cofactors Rrp47p and Mpp6p and two distinct Trf-Air-Mtr4 polyadenylation (TRAMP) complexes assists the exonuclease Rrp6p in the targeting and degradation of an aberrant messenger ribonucleoprotein particle (mRNP) in yeast.

Data show that the unwinding activity of Mtr4p is significantly stimulated by Trf4p/Air2p, but the stimulation of Mtr4p does not depend on ongoing polyadenylation.
Title: RNA unwinding by the Trf4/Air2/Mtr4 polyadenylation (TRAMP) complex.

Air1 zinc knuckles 4 and 5 and a conserved IWRXY motif are critical for the function and integrity of the Trf4/5-Air1/2-Mtr4 polyadenylation (TRAMP) RNA quality control complex.
Title: Air1 zinc knuckles 4 and 5 and a conserved IWRXY motif are critical for the function and integrity of the Trf4/5-Air1/2-Mtr4 polyadenylation (TRAMP) RNA quality control complex.

Data suggest that neither the poly(A) polymerase activity of Trf4 nor the helicase activity of Mtr4 plays a role in the enhancement of RNA degradation by the nuclear exosome component Rrp6.
Title: TRAMP complex enhances RNA degradation by the nuclear exosome component Rrp6.

The following PAP2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the PAP2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OSi03205	NM_001183369.1 Latest version!	Saccharomyces cerevisiae S288C non-canonical poly(A) polymerase PAP2 (PAP2), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

PAP2 ( NM_001183369.1 ) cDNA ORF clone, Saccharomyces cerevisiae S288C -> NP_014526.1 Saccharomyces cerevisiae S288C non-canonical poly(A) polymerase PAP2 (PAP2), partial mRNA.

Price & Availability↑

CloneID	OSi03205
Clone ID Related Accession (Same CDS sequence)	NM_001183369.1
Accession Version	NM_001183369.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1755bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-31
Organism	Saccharomyces cerevisiae S288C
Product	non-canonical poly(A) polymerase PAP2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by SGD. This record is derived from an annotated genomic sequence (NC_001147). ##Genome-Annotation-Data-START## Annotation Provider :: SGD Annotation Status :: Full Annotation Annotation Version :: R64-2-1 URL :: http://www.yeastgenome.org/ ##Genome-Annotation-Data-END## COMPLETENESS: incomplete on both ends.

ATGGGGGCAA AGAGTGTAAC AGCCTCTTCT TCAAAGAAGA TTAAAAACCG GCATAATGGG 
AAAGTGAAAA AGAGCAAGAA AATCAAGAAA GTACGGAAAC CACAGAAGTC TATATCCTTG 
AACGACGAAA ACGAAGTAGA GATTTTACCT AGTCGTAATG AGCAAGAGAC AAATAAATTA 
CCTAAAGATC ACGTCACGGC GGATGGAATT CTTGTATTAG AACATAAATC TGATGATGAT 
GAAGGATTCG ATGTCTACGA TGGACATTTC GATAACCCTA CTGATATTCC CTCGACTACT 
GAAGAAAGTA AAACGCCTTC ATTAGCAGTG CACGGGGATG AGAAGGATCT TGCTAACAAT 
GATGATTTTA TCTCTCTTTC TGCTAGTTCT GAGGATGAGC AAGCCGAACA GGAAGAGGAG 
AGGGAGAAAC AAGAATTAGA AATTAAAAAA GAAAAGCAAA AGGAAATCCT TAATACTGAT 
TATCCATGGA TTTTAAATCA TGATCACTCC AAACAGAAAG AAATCTCGGA CTGGCTAACC 
TTTGAAATCA AGGATTTTGT TGCCTATATC TCACCAAGTC GTGAAGAAAT TGAAATTCGT 
AATCAAACCA TAAGTACAAT AAGAGAGGCT GTCAAACAGT TATGGCCAGA TGCTGATTTG 
CACGTGTTTG GATCATATTC TACCGATTTG TATTTGCCTG GTTCCGATAT TGATTGCGTG 
GTAACGAGCG AACTTGGTGG TAAGGAAAGT AGGAATAACT TGTATTCGTT AGCAAGCCAT 
TTAAAAAAAA AAAATTTGGC AACAGAGGTT GAAGTTGTCG CCAAAGCTCG CGTTCCAATC 
ATCAAGTTTG TTGAGCCACA TTCAGGGATC CACATAGATG TTTCATTTGA GAGAACAAAT 
GGTATTGAAG CAGCAAAGCT TATTAGAGAG TGGTTGGACG ATACCCCCGG CCTGAGAGAA 
TTAGTCCTTA TCGTAAAACA ATTCCTACAC GCAAGAAGAC TAAATAATGT GCATACCGGT 
GGTCTTGGTG GGTTTAGTAT TATATGTCTT GTCTTTTCCT TTTTGCATAT GCACCCACGT 
ATAATAACTA ACGAAATAGA TCCAAAGGAC AACTTGGGTG TGCTCCTGAT AGAGTTTTTT 
GAACTTTATG GGAAAAATTT TGGTTATGAT GATGTTGCAC TGGGATCATC GGATGGATAT 
CCAGTATACT TTCCAAAATC GACATGGAGT GCTATTCAGC CTATTAAAAA TCCATTTTCA 
TTGGCCATTC AAGATCCAGG TGATGAATCA AATAACATCA GTCGAGGGTC CTTTAACATT 
CGAGATATTA AAAAGGCATT TGCTGGTGCT TTTGATCTGT TGACGAATAG ATGTTTTGAA 
TTACATTCAG CAACTTTTAA GGATCGATTG GGGAAAAGTA TATTGGGAAA CGTGATCAAA 
TATCGTGGAA AGGCAAGAGA TTTCAAGGAT GAAAGAGGTC TAGTACTGAA TAAGGCTATT 
ATTGAAAATG AAAACTATCA CAAGAAACGT AGCAGAATAA TTCATGATGA AGATTTTGCC 
GAAGATACAG TTACCTCCAC AGCCACCGCT ACCACAACAG ATGATGATTA TGAAATAACG 
AACCCACCTG CTAAGAAGGC TAAAATAGAA GAAAAACCTG AAAGTGAACC GGCCAAGAGA 
AATAGTGGAG AGACATATAT CACTGTCTCT AGCGAAGATG ATGATGAAGA TGGATATAAT 
CCTTATACCC TTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_014526.1
CDS	1..1755
Translation	MGAKSVTASSSKKIKNRHNGKVKKSKKIKKVRKPQKSISLNDENEVEILPSRNEQETNKLPKDHVTADGILVLEHKSDDDEGFDVYDGHFDNPTDIPSTTEESKTPSLAVHGDEKDLANNDDFISLSASSEDEQAEQEEEREKQELEIKKEKQKEILNTDYPWILNHDHSKQKEISDWLTFEIKDFVAYISPSREEIEIRNQTISTIREAVKQLWPDADLHVFGSYSTDLYLPGSDIDCVVTSELGGKESRNNLYSLASHLKKKNLATEVEVVAKARVPIIKFVEPHSGIHIDVSFERTNGIEAAKLIREWLDDTPGLRELVLIVKQFLHARRLNNVHTGGLGGFSIICLVFSFLHMHPRIITNEIDPKDNLGVLLIEFFELYGKNFGYDDVALGSSDGYPVYFPKSTWSAIQPIKNPFSLAIQDPGDESNNISRGSFNIRDIKKAFAGAFDLLTNRCFELHSATFKDRLGKSILGNVIKYRGKARDFKDERGLVLNKAIIENENYHKKRSRIIHDEDFAEDTVTSTATATTTDDDYEITNPPAKKAKIEEKPESEPAKRNSGETYITVSSEDDDEDGYNPYTL

Target ORF information:

RefSeq Version	NM_001183369.1
Organism	Saccharomyces cerevisiae S288C
Definition	Saccharomyces cerevisiae S288C non-canonical poly(A) polymerase PAP2 (PAP2), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001183369.1

ORF Insert Sequence:

ATGGGGGCAA AGAGTGTAAC AGCCTCTTCT TCAAAGAAGA TTAAAAACCG GCATAATGGG 
AAAGTGAAAA AGAGCAAGAA AATCAAGAAA GTACGGAAAC CACAGAAGTC TATATCCTTG 
AACGACGAAA ACGAAGTAGA GATTTTACCT AGTCGTAATG AGCAAGAGAC AAATAAATTA 
CCTAAAGATC ACGTCACGGC GGATGGAATT CTTGTATTAG AACATAAATC TGATGATGAT 
GAAGGATTCG ATGTCTACGA TGGACATTTC GATAACCCTA CTGATATTCC CTCGACTACT 
GAAGAAAGTA AAACGCCTTC ATTAGCAGTG CACGGGGATG AGAAGGATCT TGCTAACAAT 
GATGATTTTA TCTCTCTTTC TGCTAGTTCT GAGGATGAGC AAGCCGAACA GGAAGAGGAG 
AGGGAGAAAC AAGAATTAGA AATTAAAAAA GAAAAGCAAA AGGAAATCCT TAATACTGAT 
TATCCATGGA TTTTAAATCA TGATCACTCC AAACAGAAAG AAATCTCGGA CTGGCTAACC 
TTTGAAATCA AGGATTTTGT TGCCTATATC TCACCAAGTC GTGAAGAAAT TGAAATTCGT 
AATCAAACCA TAAGTACAAT AAGAGAGGCT GTCAAACAGT TATGGCCAGA TGCTGATTTG 
CACGTGTTTG GATCATATTC TACCGATTTG TATTTGCCTG GTTCCGATAT TGATTGCGTG 
GTAACGAGCG AACTTGGTGG TAAGGAAAGT AGGAATAACT TGTATTCGTT AGCAAGCCAT 
TTAAAAAAAA AAAATTTGGC AACAGAGGTT GAAGTTGTCG CCAAAGCTCG CGTTCCAATC 
ATCAAGTTTG TTGAGCCACA TTCAGGGATC CACATAGATG TTTCATTTGA GAGAACAAAT 
GGTATTGAAG CAGCAAAGCT TATTAGAGAG TGGTTGGACG ATACCCCCGG CCTGAGAGAA 
TTAGTCCTTA TCGTAAAACA ATTCCTACAC GCAAGAAGAC TAAATAATGT GCATACCGGT 
GGTCTTGGTG GGTTTAGTAT TATATGTCTT GTCTTTTCCT TTTTGCATAT GCACCCACGT 
ATAATAACTA ACGAAATAGA TCCAAAGGAC AACTTGGGTG TGCTCCTGAT AGAGTTTTTT 
GAACTTTATG GGAAAAATTT TGGTTATGAT GATGTTGCAC TGGGATCATC GGATGGATAT 
CCAGTATACT TTCCAAAATC GACATGGAGT GCTATTCAGC CTATTAAAAA TCCATTTTCA 
TTGGCCATTC AAGATCCAGG TGATGAATCA AATAACATCA GTCGAGGGTC CTTTAACATT 
CGAGATATTA AAAAGGCATT TGCTGGTGCT TTTGATCTGT TGACGAATAG ATGTTTTGAA 
TTACATTCAG CAACTTTTAA GGATCGATTG GGGAAAAGTA TATTGGGAAA CGTGATCAAA 
TATCGTGGAA AGGCAAGAGA TTTCAAGGAT GAAAGAGGTC TAGTACTGAA TAAGGCTATT 
ATTGAAAATG AAAACTATCA CAAGAAACGT AGCAGAATAA TTCATGATGA AGATTTTGCC 
GAAGATACAG TTACCTCCAC AGCCACCGCT ACCACAACAG ATGATGATTA TGAAATAACG 
AACCCACCTG CTAAGAAGGC TAAAATAGAA GAAAAACCTG AAAGTGAACC GGCCAAGAGA 
AATAGTGGAG AGACATATAT CACTGTCTCT AGCGAAGATG ATGATGAAGA TGGATATAAT 
CCTTATACCC TTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The nucleotide sequence of Saccharomyces cerevisiae chromosome XV.
Nature387(6632 Suppl)98-102(1997 May)
Dujon B,Albermann K,Aldea M,Alexandraki D,Ansorge W,Arino J,Benes V,Bohn C,Bolotin-Fukuhara M,Bordonn? R,Boyer J,Camasses A,Casamayor A,Casas C,Ch?ret G,Cziepluch C,Daignan-Fornier B,Dang DV,de Haan M,Delius H,Durand P,Fairhead C,Feldmann H,Gaillon L,Kleine K

Life with 6000 genes.
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)
Goffeau A,Barrell BG,Bussey H,Davis RW,Dujon B,Feldmann H,Galibert F,Hoheisel JD,Jacq C,Johnston M,Louis EJ,Mewes HW,Murakami Y,Philippsen P,Tettelin H,Oliver SG

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PAP2 cDNA ORF clone, Saccharomyces cerevisiae S288C

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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