PRP16 cDNA ORF clone, Saccharomyces cerevisiae S288C

Gene
Clones
gRNAs

Summary

Gene Symbol	PRP16
Entrez Gene ID	853961
Full Name	DEAH-box RNA helicase PRP16
Synonyms	PRP23,RNA16
Gene Type	protein-coding
Organism	Saccharomyces cerevisiae S288C

mRNA and Protein(s)

mRNA	Protein	Name
NM_001179876.3	NP_013012.3	DEAH-box RNA helicase PRP16

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	DHX38	XP_511101.3
Bos taurus (cattle)	DHX38	NP_001069266.1
Mus musculus (house mouse)	Dhx38	NP_848467.1
Danio rerio (zebrafish)	dhx38	NP_957170.1
Saccharomyces cerevisiae (baker's yeast)	PRP16	NP_013012.3
Kluyveromyces lactis	KLLA0A07733g	XP_451344.1
Eremothecium gossypii	AGOS_ADR224W	NP_984320.1
Oryza sativa (rice)	Os07g0508000	NP_001059742.1
Xenopus tropicalis (tropical clawed frog)	dhx38	XP_002937626.2
	DHX38	XP_001097681.2
Canis lupus familiaris (dog)	DHX38	XP_005620809.1
Gallus gallus (chicken)	DHX38	NP_001025753.1
Caenorhabditis elegans (roundworm)	mog-1	NP_499212.1
Schizosaccharomyces pombe (fission yeast)	prp16	NP_595890.2
Rattus norvegicus (Norway rat)	Dhx38	NP_001099655.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP008528	XP_316912.4
Homo sapiens (human)	DHX38	NP_054722.2
Drosophila melanogaster (fruit fly)	l(1)G0007	NP_572947.1
Magnaporthe oryzae (rice blast fungus)	MGG_11351	XP_003713740.1
Neurospora crassa	NCU02728	XP_965113.1
Arabidopsis thaliana (thale cress)	EMB3011	NP_196805.2

Gene Ontology
Bibliography

Related articles in PubMed

Life with 6000 genes.
Goffeau A, Barrell BG, Bussey H, Davis RW, Dujon B, Feldmann H, Galibert F, Hoheisel JD, Jacq C, Johnston M, Louis EJ, Mewes HW, Murakami Y, Philippsen P, Tettelin H, Oliver SG
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)

Complete DNA sequence of yeast chromosome XI.
Dujon B, Alexandraki D, Andr? B, Ansorge W, Baladron V, Ballesta JP, Banrevi A, Bolle PA, Bolotin-Fukuhara M, Bossier P, Bou G, Boyer J, Bultrago MJ, Cheret G, Colleaux L, Dalgnan-Fornler B, del Rey F, Dlon C, Domdey H, D?sterhoft A, D?sterhus S, Entlan KD, Erfle H, Esteban PF, Feldmann H, Fernandes L, Robo GM, Fritz C, Fukuhara H, Gabel C, Gaillon L, Carcia-Cantalejo JM, Garcia-Ramirez JJ, Gent NE, Ghazvini M, Goffeau A, Gonzal?z A, Grothues D, Guerreiro P, Hegemann J, Hewitt N, Hilger F, Hollenberg CP, Horaitis O, Indge KJ, Jacquier A, James CM, Jauniaux C, Jimenez A, Keuchel H, Kirchrath L, Kleine K, K?tter P, Legrain P, Liebl S, Louis EJ, Maia e Silva A, Marck C, Monnier AL, M?stl D, M?ller S, Obermaier B, Oliver SG, Pallier C, Pascolo S, Pfeiffer F, Philippsen P, Planta RJ, Pohl FM, Pohl TM, P?hlmann R, Portetelle D, Purnelle B, Puzos V, Ramezani Rad M, Rasmussen SW, Remacha M, Revuelta JL, Richard GF, Rieger M, Rodrigues-Pousada C, Rose M, Rupp T, Santos MA, Schwager C, Sensen C, Skala J, Soares H, Sor F, Stegemann J, Tettelin H, Thierry A, Tzermia M, Urrestarazu LA, van Dyck L, Van Vliet-Reedijk JC, Valens M, Vandenbo M, Vilela C, Vissers S, von Wettstein D, Voss H, Wiemann S, Xu G, Zimmermann J, Haasemann M, Becker I, Mewes HW
Nature369(6479)371-8(1994 Jun)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

In the process of pre-mRNA splicing, Cwc25 binds tightly to the spliceosome after the reaction and is then removed from the spliceosome, which normally requires Prp16 and ATP hydrolysis, to allow the occurrence of the second reaction.
Title: A central role of Cwc25 in spliceosome dynamics during the catalytic phase of pre-mRNA splicing.

At the catalytic stage of splicing, suboptimal splice sites are repressed by the DEAH-box ATPases Prp16 and Prp22. study shows that these ATPases function further by enabling the spliceosome to search for and utilize alternative branch sites and 3' splice sites.
Title: Spliceosomal DEAH-Box ATPases Remodel Pre-mRNA to Activate Alternative Splice Sites.

Results indicate that the prp16-302 mutation in Prp16 stabilizes Cwc2 interactions with U6 snRNA and destabilizes Cwc2 interactions with pre-mRNA, indicating antagonistic functions of Cwc2 and Prp16.
Title: Remodeling of U2-U6 snRNA helix I during pre-mRNA splicing by Prp16 and the NineTeen Complex protein Cwc2.

In the absence of the C domain, Yju2-N domain is not stably associated with the spliceosome after lariat formation, and thus bypasses the need for Prp16.
Title: A weak spliceosome-binding domain of Yju2 functions in the first step and bypasses Prp16 in the second step of splicing.

NTR complex catalyzes the disassembly of affinity-purified spliceosomes arrested specifically after the ATP-dependent action of DEAH-box Prp2, Prp16, or Prp22 but not at steps before the action of these ATPases or upon their binding to the spliceosome.
Title: Link of NTR-mediated spliceosome disassembly with DEAH-box ATPases Prp2, Prp16, and Prp22.

The following PRP16 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the PRP16 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OSi03138	NM_001179876.3 Latest version!	Saccharomyces cerevisiae S288C DEAH-box RNA helicase PRP16 (PRP16), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

PRP16 ( NM_001179876.3 ) cDNA ORF clone, Saccharomyces cerevisiae S288C -> NP_013012.3 Saccharomyces cerevisiae S288C DEAH-box RNA helicase PRP16 (PRP16), partial mRNA.

Price & Availability↑

CloneID	OSi03138
Clone ID Related Accession (Same CDS sequence)	NM_001179876.3
Accession Version	NM_001179876.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3216bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-31
Organism	Saccharomyces cerevisiae S288C
Product	DEAH-box RNA helicase PRP16
Comment	Comment: REVIEWED REFSEQ: This record has been curated by SGD. This record is derived from an annotated genomic sequence (NC_001143). On Jul 30, 2012 this sequence version replaced NM_001179876.2. ##Genome-Annotation-Data-START## Annotation Provider :: SGD Annotation Status :: Full Annotation Annotation Version :: R64-2-1 URL :: http://www.yeastgenome.org/ ##Genome-Annotation-Data-END## COMPLETENESS: incomplete on both ends.

ATGGGTCATT CGGGGCGTGA GGAAAGGATA AAAGACATAT TTAAGGAACT TACGTCAAAA 
GAACTAACGC CTGGGTTGCT TTTGACACTG CAGAAACTAG CGCAAAAGCC GAATACTAAT 
CTAGAACAGT TTATTGCCAG CTGTAAAGCT TTGACGAAAC TCAGCAGTAA CAATCCAATC 
ATTTTTAATG AACTTCTGGA GCTCCTCAAG AATAAATCTG AAGAGGACTC GACAGGCCCA 
AAGAAAATCG CCCCTTCTAT AAATAAGAGA AAGAAATTTA AGATCCAGTT AGACCTTGAC 
GATAATGAAG ATGAGCTAGA TTCTCCAGTT CAAAAAAAAC CAGCACCTAC ACGGACGTTG 
TTCAAGAGAA TAGATAAACT AAAGGCCAAA CAGTTGAGGC AGTATTCGCC CACCGTGAAG 
GATCCTTCTC CAAATAGTGA ACAGCAAACA CAGAATGGTC ATGCTGAAAC GAAGGACTAT 
GAACCGACTC GAAGCGAAGT AGTTGAGGAG GACAGAGAAT GGTATGATAA TGATGATGAT 
TATGGAAATT TGGTGCCTGA ACCTCTGTCT GAATTGCCTG AGGAAGCGAA ACTACTACCT 
GTCATTAGGA ATATCGACAA CGATGATGCC TTACGAAATA CCGTTCAATT ATACCCCATA 
CCACTCAAGC AAAGAATGGA ATGGATCCCT CCTTTTTTAT CAAAGTTTGC TCTTGAAAAC 
AAAGTGCCCA CTTCCATAAT TATCGGGTCC ATCTCTGAGA CGTCCAGCCA AGTATCCGCT 
TTATCAATGG TGAATCCATT TAGGAATCCT GACAGTGAAT TTTCTGCGAA CGCCAAACGA 
GGTAGTAAGC TGGTTGCCTT GAGAAGGATA AACATGGAAC ATATTCAACA ATCTCGAGAC 
AACACCACTG TTTTGAACAC TGCCATGGGG GAAGTACTAG GCTTGGAGAA TAACAATAAA 
GCAAAAGATA AGAGCAACCA AAAGATTTGC GACGATACAG CTCTTTTCAC GCCATCAAAA 
GATGACATTA AACATACTAA AGAGCAACTG CCTGTTTTCC GCTGCAGATC TCAATTGTTA 
TCATTGATAA GAGAAAATCA AGTAGTGGTG ATAATTGGTG AAACGGGCTC AGGTAAAACC 
ACGCAACTTG CACAGTATTT ATATGAAGAA GGATATGCCA ACGATAGGGG GAAATCTATT 
GTTGTCACAC AGCCGAGAAG AGTAGCAGCC ATATCCGTTG CAAAAAGGGT TGCAATGGAA 
ATGCAAGTTC CATTGGGTAA GGAAGTAGGC TATTCTATAA GGTTTGAAGA TGTGACGGAT 
TCTGAGTGTA CAAAACTTAA GTTTGTAACG GATGGTATTC TACTCAGAGA GACCTTATTG 
GATGATACAC TCGATAAATA TTCGTGTGTT ATTATTGATG AAGCTCATGA AAGGTCATTA 
AATACAGACA TTTTACTAGG TTTTTTCAAG ATTTTATTAG CACGTAGAAG AGATCTAAAA 
CTCATAATCA CTTCAGCAAC AATGAATGCG AAGAAGTTCT CCGCATTTTT TGGTAATGCT 
CCTCAGTTCA CAATTCCTGG AAGAACTTTC CCCGTACAGA CCATATATAC TTCCAACCCT 
GTTCAAGACT ACGTAGAAGC TGCAGTTTCT CAGGCTGTGA AAATTCATTT AGCAAACGAT 
TGTTCAAGCG GTGATATTCT CATTTTCATG ACAGGTCAAG AAGATATTGA AACAACGTTC 
GACACTCTGC AAGAAAAGTT TTTGCAAGTA TATTCTAAAA AGTTTGGTAC CGCAAACTTT 
GAAGAGATCA ATGATATTGA AATTTTACCA ATCTACTCGG CTTTACCTGC AGATTTGCAA 
TTCAAAATTT TTCAGGATCT ACATGGCACT AAAAGGAAAA TTATAATTGC GACAAATATT 
GCAGAGACTT CATTGACCAT TAAAGGTATT AGATATGTCA TTGATTGTGG TTACTCTAAA 
CTGAAAGTTT ACAATCCAAA GATTGGGCTT GATAGTTTAG TAATAACGCC CATATCAAAA 
GCTAACGCCG ACCAAAGATC CGGAAGAGCG GGAAGAACTG CGCCAGGCAC TGCATATCGA 
TTATACACGG AAGATACATT CAAAGAAGAT ATGTACTTAC AGACAATACC TGAGATCCAA 
AGAACGAATT TGTCAAACAC CCTTTTACTG CTAAAATCTC TGGACGTTAC TGACGAATTG 
AGTAAGTTCC CGTTCATTGA TAAACCACCC TTACAAACAT TTTTATCCTC ATTGTATGAG 
TTATGGTTCA TAGGGGCTAT AGACACTAGT GGACAATTGA CTCCATTGGG ATTACAGATG 
GCAAAATTTC CCCTGCAACC TTCCCTTTCC AAAATTTTAT TAATTGCAGT TAGAAACGGC 
TGCAGTGATG AAATGTTAAC AATAGTTTCC ATGCTTTCTG TTCCACAAGT CTTTTATAGA 
CCCAAGGAAA GACAGAAAGA AGCAGATATT GCTAGAAATA AATTTTTTAT CGCGAAATCA 
GATCACTTAA CGTTACTTAA TGTTTTTGAA CAATGGAGGG CAAATAATTT TAGTTCGCAT 
TGGTGTAACA AACATTTTGT TCAATACAAA TCACTTGTCA GGGCAAGAGA CATTCGAGAC 
CAGTTGCTAA CAATACTAAA ATCTCAAAAA ATTCCGGTCA TTTCCTCCGG TAAGGACTGG 
GATATTATTA AAAAATGTAT TTGTTCAGGA TTTGCTCATC AAGCAGCCAA AATAACAGGA 
CTACGAAATT ACGTTCATTT AAAAACTGGC GTAAGTGTTC AACTACACCC TACAAGTGCA 
TTACACGGAT TGGGGGATTT ACCCCCTTAC GTGGTATATC ATGAATTATT AATGACTAGC 
AAAGAATACA TTTGTTGTGT TACTTCTGTA GATCCATTCT GGTTAATGGA ATATGGTGGG 
TTACTCTACG ACATCAAGCG TATTAAAAAT GATCAGGAGG CCACAACTAC AGGCCTCTTT 
GGTGAACACT ATGAACATAC TCTGGACAAG GTTGAAGATG ATATTGATAT CAATATTAGG 
AGATGTAAGG ATATGAGAGA CAGTGTGATA CAGGAGCTCA AAATGACCGA CAATTCAAAC 
AAAGAAGACA AAAAGCAAAA AACGAAAAAG CAAAATATAC TGAACGGCAA AGAAAATTCA 
ATGAAACCTT TCAAAAGAAG GAAGCCTTTT TTTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_013012.3
CDS	1..3216
Translation	MGHSGREERIKDIFKELTSKELTPGLLLTLQKLAQKPNTNLEQFIASCKALTKLSSNNPIIFNELLELLKNKSEEDSTGPKKIAPSINKRKKFKIQLDLDDNEDELDSPVQKKPAPTRTLFKRIDKLKAKQLRQYSPTVKDPSPNSEQQTQNGHAETKDYEPTRSEVVEEDREWYDNDDDYGNLVPEPLSELPEEAKLLPVIRNIDNDDALRNTVQLYPIPLKQRMEWIPPFLSKFALENKVPTSIIIGSISETSSQVSALSMVNPFRNPDSEFSANAKRGSKLVALRRINMEHIQQSRDNTTVLNTAMGEVLGLENNNKAKDKSNQKICDDTALFTPSKDDIKHTKEQLPVFRCRSQLLSLIRENQVVVIIGETGSGKTTQLAQYLYEEGYANDRGKSIVVTQPRRVAAISVAKRVAMEMQVPLGKEVGYSIRFEDVTDSECTKLKFVTDGILLRETLLDDTLDKYSCVIIDEAHERSLNTDILLGFFKILLARRRDLKLIITSATMNAKKFSAFFGNAPQFTIPGRTFPVQTIYTSNPVQDYVEAAVSQAVKIHLANDCSSGDILIFMTGQEDIETTFDTLQEKFLQVYSKKFGTANFEEINDIEILPIYSALPADLQFKIFQDLHGTKRKIIIATNIAETSLTIKGIRYVIDCGYSKLKVYNPKIGLDSLVITPISKANADQRSGRAGRTAPGTAYRLYTEDTFKEDMYLQTIPEIQRTNLSNTLLLLKSLDVTDELSKFPFIDKPPLQTFLSSLYELWFIGAIDTSGQLTPLGLQMAKFPLQPSLSKILLIAVRNGCSDEMLTIVSMLSVPQVFYRPKERQKEADIARNKFFIAKSDHLTLLNVFEQWRANNFSSHWCNKHFVQYKSLVRARDIRDQLLTILKSQKIPVISSGKDWDIIKKCICSGFAHQAAKITGLRNYVHLKTGVSVQLHPTSALHGLGDLPPYVVYHELLMTSKEYICCVTSVDPFWLMEYGGLLYDIKRIKNDQEATTTGLFGEHYEHTLDKVEDDIDINIRRCKDMRDSVIQELKMTDNSNKEDKKQKTKKQNILNGKENSMKPFKRRKPFF

Target ORF information:

RefSeq Version	NM_001179876.3
Organism	Saccharomyces cerevisiae S288C
Definition	Saccharomyces cerevisiae S288C DEAH-box RNA helicase PRP16 (PRP16), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001179876.3

ORF Insert Sequence:

ATGGGTCATT CGGGGCGTGA GGAAAGGATA AAAGACATAT TTAAGGAACT TACGTCAAAA 
GAACTAACGC CTGGGTTGCT TTTGACACTG CAGAAACTAG CGCAAAAGCC GAATACTAAT 
CTAGAACAGT TTATTGCCAG CTGTAAAGCT TTGACGAAAC TCAGCAGTAA CAATCCAATC 
ATTTTTAATG AACTTCTGGA GCTCCTCAAG AATAAATCTG AAGAGGACTC GACAGGCCCA 
AAGAAAATCG CCCCTTCTAT AAATAAGAGA AAGAAATTTA AGATCCAGTT AGACCTTGAC 
GATAATGAAG ATGAGCTAGA TTCTCCAGTT CAAAAAAAAC CAGCACCTAC ACGGACGTTG 
TTCAAGAGAA TAGATAAACT AAAGGCCAAA CAGTTGAGGC AGTATTCGCC CACCGTGAAG 
GATCCTTCTC CAAATAGTGA ACAGCAAACA CAGAATGGTC ATGCTGAAAC GAAGGACTAT 
GAACCGACTC GAAGCGAAGT AGTTGAGGAG GACAGAGAAT GGTATGATAA TGATGATGAT 
TATGGAAATT TGGTGCCTGA ACCTCTGTCT GAATTGCCTG AGGAAGCGAA ACTACTACCT 
GTCATTAGGA ATATCGACAA CGATGATGCC TTACGAAATA CCGTTCAATT ATACCCCATA 
CCACTCAAGC AAAGAATGGA ATGGATCCCT CCTTTTTTAT CAAAGTTTGC TCTTGAAAAC 
AAAGTGCCCA CTTCCATAAT TATCGGGTCC ATCTCTGAGA CGTCCAGCCA AGTATCCGCT 
TTATCAATGG TGAATCCATT TAGGAATCCT GACAGTGAAT TTTCTGCGAA CGCCAAACGA 
GGTAGTAAGC TGGTTGCCTT GAGAAGGATA AACATGGAAC ATATTCAACA ATCTCGAGAC 
AACACCACTG TTTTGAACAC TGCCATGGGG GAAGTACTAG GCTTGGAGAA TAACAATAAA 
GCAAAAGATA AGAGCAACCA AAAGATTTGC GACGATACAG CTCTTTTCAC GCCATCAAAA 
GATGACATTA AACATACTAA AGAGCAACTG CCTGTTTTCC GCTGCAGATC TCAATTGTTA 
TCATTGATAA GAGAAAATCA AGTAGTGGTG ATAATTGGTG AAACGGGCTC AGGTAAAACC 
ACGCAACTTG CACAGTATTT ATATGAAGAA GGATATGCCA ACGATAGGGG GAAATCTATT 
GTTGTCACAC AGCCGAGAAG AGTAGCAGCC ATATCCGTTG CAAAAAGGGT TGCAATGGAA 
ATGCAAGTTC CATTGGGTAA GGAAGTAGGC TATTCTATAA GGTTTGAAGA TGTGACGGAT 
TCTGAGTGTA CAAAACTTAA GTTTGTAACG GATGGTATTC TACTCAGAGA GACCTTATTG 
GATGATACAC TCGATAAATA TTCGTGTGTT ATTATTGATG AAGCTCATGA AAGGTCATTA 
AATACAGACA TTTTACTAGG TTTTTTCAAG ATTTTATTAG CACGTAGAAG AGATCTAAAA 
CTCATAATCA CTTCAGCAAC AATGAATGCG AAGAAGTTCT CCGCATTTTT TGGTAATGCT 
CCTCAGTTCA CAATTCCTGG AAGAACTTTC CCCGTACAGA CCATATATAC TTCCAACCCT 
GTTCAAGACT ACGTAGAAGC TGCAGTTTCT CAGGCTGTGA AAATTCATTT AGCAAACGAT 
TGTTCAAGCG GTGATATTCT CATTTTCATG ACAGGTCAAG AAGATATTGA AACAACGTTC 
GACACTCTGC AAGAAAAGTT TTTGCAAGTA TATTCTAAAA AGTTTGGTAC CGCAAACTTT 
GAAGAGATCA ATGATATTGA AATTTTACCA ATCTACTCGG CTTTACCTGC AGATTTGCAA 
TTCAAAATTT TTCAGGATCT ACATGGCACT AAAAGGAAAA TTATAATTGC GACAAATATT 
GCAGAGACTT CATTGACCAT TAAAGGTATT AGATATGTCA TTGATTGTGG TTACTCTAAA 
CTGAAAGTTT ACAATCCAAA GATTGGGCTT GATAGTTTAG TAATAACGCC CATATCAAAA 
GCTAACGCCG ACCAAAGATC CGGAAGAGCG GGAAGAACTG CGCCAGGCAC TGCATATCGA 
TTATACACGG AAGATACATT CAAAGAAGAT ATGTACTTAC AGACAATACC TGAGATCCAA 
AGAACGAATT TGTCAAACAC CCTTTTACTG CTAAAATCTC TGGACGTTAC TGACGAATTG 
AGTAAGTTCC CGTTCATTGA TAAACCACCC TTACAAACAT TTTTATCCTC ATTGTATGAG 
TTATGGTTCA TAGGGGCTAT AGACACTAGT GGACAATTGA CTCCATTGGG ATTACAGATG 
GCAAAATTTC CCCTGCAACC TTCCCTTTCC AAAATTTTAT TAATTGCAGT TAGAAACGGC 
TGCAGTGATG AAATGTTAAC AATAGTTTCC ATGCTTTCTG TTCCACAAGT CTTTTATAGA 
CCCAAGGAAA GACAGAAAGA AGCAGATATT GCTAGAAATA AATTTTTTAT CGCGAAATCA 
GATCACTTAA CGTTACTTAA TGTTTTTGAA CAATGGAGGG CAAATAATTT TAGTTCGCAT 
TGGTGTAACA AACATTTTGT TCAATACAAA TCACTTGTCA GGGCAAGAGA CATTCGAGAC 
CAGTTGCTAA CAATACTAAA ATCTCAAAAA ATTCCGGTCA TTTCCTCCGG TAAGGACTGG 
GATATTATTA AAAAATGTAT TTGTTCAGGA TTTGCTCATC AAGCAGCCAA AATAACAGGA 
CTACGAAATT ACGTTCATTT AAAAACTGGC GTAAGTGTTC AACTACACCC TACAAGTGCA 
TTACACGGAT TGGGGGATTT ACCCCCTTAC GTGGTATATC ATGAATTATT AATGACTAGC 
AAAGAATACA TTTGTTGTGT TACTTCTGTA GATCCATTCT GGTTAATGGA ATATGGTGGG 
TTACTCTACG ACATCAAGCG TATTAAAAAT GATCAGGAGG CCACAACTAC AGGCCTCTTT 
GGTGAACACT ATGAACATAC TCTGGACAAG GTTGAAGATG ATATTGATAT CAATATTAGG 
AGATGTAAGG ATATGAGAGA CAGTGTGATA CAGGAGCTCA AAATGACCGA CAATTCAAAC 
AAAGAAGACA AAAAGCAAAA AACGAAAAAG CAAAATATAC TGAACGGCAA AGAAAATTCA 
ATGAAACCTT TCAAAAGAAG GAAGCCTTTT TTTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Life with 6000 genes.
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)
Goffeau A,Barrell BG,Bussey H,Davis RW,Dujon B,Feldmann H,Galibert F,Hoheisel JD,Jacq C,Johnston M,Louis EJ,Mewes HW,Murakami Y,Philippsen P,Tettelin H,Oliver SG

Complete DNA sequence of yeast chromosome XI.
Nature369(6479)371-8(1994 Jun)
Dujon B,Alexandraki D,Andr? B,Ansorge W,Baladron V,Ballesta JP,Banrevi A,Bolle PA,Bolotin-Fukuhara M,Bossier P,Bou G,Boyer J,Bultrago MJ,Cheret G,Colleaux L,Dalgnan-Fornler B,del Rey F,Dlon C,Domdey H,D?sterhoft A,D?sterhus S,Entlan KD,Erfle H,Esteban PF,Feldmann H,Fernandes L,Robo GM,Fritz C,Fukuhara H,Gabel C,Gaillon L,Carcia-Cantalejo JM,Garcia-Ramirez JJ,Gent NE,Ghazvini M,Goffeau A,Gonzal?z A,Grothues D,Guerreiro P,Hegemann J,Hewitt N,Hilger F,Hollenberg CP,Horaitis O,Indge KJ,Jacquier A,James CM,Jauniaux C,Jimenez A,Keuchel H,Kirchrath L,Kleine K,K?tter P,Legrain P,Liebl S,Louis EJ,Maia e Silva A,Marck C,Monnier AL,M?stl D,M?ller S,Obermaier B,Oliver SG,Pallier C,Pascolo S,Pfeiffer F,Philippsen P,Planta RJ,Pohl FM,Pohl TM,P?hlmann R,Portetelle D,Purnelle B,Puzos V,Ramezani Rad M,Rasmussen SW,Remacha M,Revuelta JL,Richard GF,Rieger M,Rodrigues-Pousada C,Rose M,Rupp T,Santos MA,Schwager C,Sensen C,Skala J,Soares H,Sor F,Stegemann J,Tettelin H,Thierry A,Tzermia M,Urrestarazu LA,van Dyck L,Van Vliet-Reedijk JC,Valens M,Vandenbo M,Vilela C,Vissers S,von Wettstein D,Voss H,Wiemann S,Xu G,Zimmermann J,Haasemann M,Becker I,Mewes HW

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PRP16 cDNA ORF clone, Saccharomyces cerevisiae S288C

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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