SSH4 cDNA ORF clone, Saccharomyces cerevisiae S288C

Gene
Clones
gRNAs

Summary

Gene Symbol	SSH4
Entrez Gene ID	853735
Full Name	Ssh4p
Synonyms	MLF4
Gene Type	protein-coding
Organism	Saccharomyces cerevisiae S288C

mRNA and Protein(s)

mRNA	Protein	Name
NM_001179690.1	NP_012798.1	Ssh4p

Pathways from BioSystems

Homologies

Saccharomyces cerevisiae (baker's yeast)	SSH4	NP_012798.1
Kluyveromyces lactis	KLLA0B12760g	XP_452099.1

Gene Ontology
Bibliography

Related articles in PubMed

Life with 6000 genes.
Goffeau A, Barrell BG, Bussey H, Davis RW, Dujon B, Feldmann H, Galibert F, Hoheisel JD, Jacq C, Johnston M, Louis EJ, Mewes HW, Murakami Y, Philippsen P, Tettelin H, Oliver SG
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)

Complete DNA sequence of yeast chromosome XI.
Dujon B, Alexandraki D, Andr? B, Ansorge W, Baladron V, Ballesta JP, Banrevi A, Bolle PA, Bolotin-Fukuhara M, Bossier P, Bou G, Boyer J, Bultrago MJ, Cheret G, Colleaux L, Dalgnan-Fornler B, del Rey F, Dlon C, Domdey H, D?sterhoft A, D?sterhus S, Entlan KD, Erfle H, Esteban PF, Feldmann H, Fernandes L, Robo GM, Fritz C, Fukuhara H, Gabel C, Gaillon L, Carcia-Cantalejo JM, Garcia-Ramirez JJ, Gent NE, Ghazvini M, Goffeau A, Gonzal?z A, Grothues D, Guerreiro P, Hegemann J, Hewitt N, Hilger F, Hollenberg CP, Horaitis O, Indge KJ, Jacquier A, James CM, Jauniaux C, Jimenez A, Keuchel H, Kirchrath L, Kleine K, K?tter P, Legrain P, Liebl S, Louis EJ, Maia e Silva A, Marck C, Monnier AL, M?stl D, M?ller S, Obermaier B, Oliver SG, Pallier C, Pascolo S, Pfeiffer F, Philippsen P, Planta RJ, Pohl FM, Pohl TM, P?hlmann R, Portetelle D, Purnelle B, Puzos V, Ramezani Rad M, Rasmussen SW, Remacha M, Revuelta JL, Richard GF, Rieger M, Rodrigues-Pousada C, Rose M, Rupp T, Santos MA, Schwager C, Sensen C, Skala J, Soares H, Sor F, Stegemann J, Tettelin H, Thierry A, Tzermia M, Urrestarazu LA, van Dyck L, Van Vliet-Reedijk JC, Valens M, Vandenbo M, Vilela C, Vissers S, von Wettstein D, Voss H, Wiemann S, Xu G, Zimmermann J, Haasemann M, Becker I, Mewes HW
Nature369(6479)371-8(1994 Jun)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Ear1p/Ssh4p recruit Rsp5p and assist it in its function at multivesicular bodies by directing the ubiquitylation of specific cargoes.
Title: Ear1p and Ssh4p are new adaptors of the ubiquitin ligase Rsp5p for cargo ubiquitylation and sorting at multivesicular bodies.

model in which Ssh4, Rcr2, and presumably Rcr1, function within the endosome-vacuole trafficking pathway, where they affect events that determine whether plasma membrane proteins are degraded or routed to the plasma membrane
Title: Ssh4, Rcr2 and Rcr1 affect plasma membrane transporter activity in Saccharomyces cerevisiae.

The following SSH4 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the SSH4 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OSi02943	NM_001179690.1 Latest version!	Saccharomyces cerevisiae S288C Ssh4p (SSH4), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

SSH4 ( NM_001179690.1 ) cDNA ORF clone, Saccharomyces cerevisiae S288C -> NP_012798.1 Saccharomyces cerevisiae S288C Ssh4p (SSH4), partial mRNA.

Price & Availability↑

CloneID	OSi02943
Clone ID Related Accession (Same CDS sequence)	NM_001179690.1
Accession Version	NM_001179690.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1740bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-31
Organism	Saccharomyces cerevisiae S288C
Product	Ssh4p
Comment	Comment: REVIEWED REFSEQ: This record has been curated by SGD. This record is derived from an annotated genomic sequence (NC_001143). ##Genome-Annotation-Data-START## Annotation Provider :: SGD Annotation Status :: Full Annotation Annotation Version :: R64-2-1 URL :: http://www.yeastgenome.org/ ##Genome-Annotation-Data-END## COMPLETENESS: incomplete on both ends.

ATGTATGTCA CTTTTAATGA GGCTTTGGAT AGTTCTTTTG GTAATCTAGA GAGTCCGAAT 
CATGACTTCA AAGTAGGTGA CCCTAACATG GTACCAACTC CCCCTATGGA TTCAGATTCT 
GCAGCAATTA GTTTGGCTTT CTTAATAAGT CTTTCTATTA CGTTTGCAAT ATTAATGCTC 
ATCTTGGTAG TAATAGCGGC ATATGTCACC TTTTGTGGTG ATGATGAATC AGAATACGAC 
GAAGAGAACG CCTTAGGTAC ACGAACTTCA GGAACTCTGC ACTCTTTGTT TGGTAAAAAA 
CATAGTGGCA TCTTGTTGGA TTCGAGTTTT GCATCCCCTG GCGGGTTTGA TGATGAGATC 
GTTCTTCAGG AAAGAGAACT TGAAGAATTA CCAAAGATGT CAGCGTACGA GGTTGAGTTG 
TATATAAGAG CGAAAGAGTT TCAAATGATG AGCCCGCCAA TGGTGAAGGA CTTTGGCACG 
TATTTGGACA GTGACGACCA GCAGTTTATT AAGGATCGTG GCATTCAAAG CTATTTTTTG 
CTACCAAGCA TTAATGATAA TATAGACGAA TATGGAAACT TTCTACCCAG TTTTATTGTC 
CAGGATAAAC TAGATATTCA ATTTTCAAAG TTCAATAAGA GCTCATCCAC TGTAATGAAC 
TATCCCCTAC CTCACAATAG GAAGGACGCA GTGTATTTCG AAGTTAAAAT TTTCAGACAT 
ATTCAAAAGT CCAATAGTAT ATTCAGTATA GGTTTGACTA CTGTACCATA TCCTTATTTC 
AGGGTACCGG GCATGGCCAA ATACTCCATT GCATACGAAT CCACAGGTAA ACTGAGGATA 
AATAACCCCT TCACTGCCAG CACATTATTA CCAAAATTGG AAGAAGGTGA CACGGTAGGA 
TTCGGTTACA GATATAAAAC AGGTACAATC TTCATTACAC ATAATGGGAA AAAATTAATG 
GATGTAACAC AAAATATTGG CATTGATCTT TTCATTGGAA TTGGCGCGTT TAATGCCGCA 
TATACAAGAA CATATACGAG GGATGGTCTA TTGGAAGACC CGGATAATGT TAGCTTCCGT 
GAAGCTCTCT CTGAAGGCAA AGATATTGAA GTCGCCAAAG ATCTTCAAAG AGTTCACGAT 
CCACATGATG AAAGTGATGA AATGACGTCA GATGAGGTTG AATTACATGT TAATTTGGGC 
CAAGTTGGGT TCGTCTTTAT AGAAGCCAAT GTGAAAAAAT ATGCGTTTGG GAGTGTTTAT 
GGCCAAATTG GAATTCCTCC TGCGTATAAT GGAACCGAGA TCAAGAAGGA TACTATTTTA 
CAGAAAGGAG AAGAATTGCC ACCAAGATAT GCTGACACTG ATAATTTCTT TGGTAGTATG 
AAGGTAAAAG AAGGGTCATC CTCTAGGATA ACGGCGCAAA CTAGTAAGCC CCTGTGGTCT 
GTTGGGACGT ATGAAAGAAT CTCTTCTAAC TTTGACAGAG AAAATAATGT TTACCACGAC 
AGTCTTGAAA CCGACGATAA CAACACCGAT AACAATGTTA ATAACAACGA TGAGAACGCT 
GGTTGCAATG AAAATTCGCC ATTATTGGAA GATGATGGCA ATAAAAGACC GGAAAATTCA 
AATACCCCCC GTGAAGTATC AGATGGAGCT ATCAATAAGA ACCCTAGAAA TAAATCTACT 
AAAAAACGTC AAAGAAACAG AGGCAAATCT TCTAAAAAGA AGAACAGATC GAGAAAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_012798.1
CDS	1..1740
Translation	MYVTFNEALDSSFGNLESPNHDFKVGDPNMVPTPPMDSDSAAISLAFLISLSITFAILMLILVVIAAYVTFCGDDESEYDEENALGTRTSGTLHSLFGKKHSGILLDSSFASPGGFDDEIVLQERELEELPKMSAYEVELYIRAKEFQMMSPPMVKDFGTYLDSDDQQFIKDRGIQSYFLLPSINDNIDEYGNFLPSFIVQDKLDIQFSKFNKSSSTVMNYPLPHNRKDAVYFEVKIFRHIQKSNSIFSIGLTTVPYPYFRVPGMAKYSIAYESTGKLRINNPFTASTLLPKLEEGDTVGFGYRYKTGTIFITHNGKKLMDVTQNIGIDLFIGIGAFNAAYTRTYTRDGLLEDPDNVSFREALSEGKDIEVAKDLQRVHDPHDESDEMTSDEVELHVNLGQVGFVFIEANVKKYAFGSVYGQIGIPPAYNGTEIKKDTILQKGEELPPRYADTDNFFGSMKVKEGSSSRITAQTSKPLWSVGTYERISSNFDRENNVYHDSLETDDNNTDNNVNNNDENAGCNENSPLLEDDGNKRPENSNTPREVSDGAINKNPRNKSTKKRQRNRGKSSKKKNRSRK

Target ORF information:

RefSeq Version	NM_001179690.1
Organism	Saccharomyces cerevisiae S288C
Definition	Saccharomyces cerevisiae S288C Ssh4p (SSH4), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001179690.1

ORF Insert Sequence:

ATGTATGTCA CTTTTAATGA GGCTTTGGAT AGTTCTTTTG GTAATCTAGA GAGTCCGAAT 
CATGACTTCA AAGTAGGTGA CCCTAACATG GTACCAACTC CCCCTATGGA TTCAGATTCT 
GCAGCAATTA GTTTGGCTTT CTTAATAAGT CTTTCTATTA CGTTTGCAAT ATTAATGCTC 
ATCTTGGTAG TAATAGCGGC ATATGTCACC TTTTGTGGTG ATGATGAATC AGAATACGAC 
GAAGAGAACG CCTTAGGTAC ACGAACTTCA GGAACTCTGC ACTCTTTGTT TGGTAAAAAA 
CATAGTGGCA TCTTGTTGGA TTCGAGTTTT GCATCCCCTG GCGGGTTTGA TGATGAGATC 
GTTCTTCAGG AAAGAGAACT TGAAGAATTA CCAAAGATGT CAGCGTACGA GGTTGAGTTG 
TATATAAGAG CGAAAGAGTT TCAAATGATG AGCCCGCCAA TGGTGAAGGA CTTTGGCACG 
TATTTGGACA GTGACGACCA GCAGTTTATT AAGGATCGTG GCATTCAAAG CTATTTTTTG 
CTACCAAGCA TTAATGATAA TATAGACGAA TATGGAAACT TTCTACCCAG TTTTATTGTC 
CAGGATAAAC TAGATATTCA ATTTTCAAAG TTCAATAAGA GCTCATCCAC TGTAATGAAC 
TATCCCCTAC CTCACAATAG GAAGGACGCA GTGTATTTCG AAGTTAAAAT TTTCAGACAT 
ATTCAAAAGT CCAATAGTAT ATTCAGTATA GGTTTGACTA CTGTACCATA TCCTTATTTC 
AGGGTACCGG GCATGGCCAA ATACTCCATT GCATACGAAT CCACAGGTAA ACTGAGGATA 
AATAACCCCT TCACTGCCAG CACATTATTA CCAAAATTGG AAGAAGGTGA CACGGTAGGA 
TTCGGTTACA GATATAAAAC AGGTACAATC TTCATTACAC ATAATGGGAA AAAATTAATG 
GATGTAACAC AAAATATTGG CATTGATCTT TTCATTGGAA TTGGCGCGTT TAATGCCGCA 
TATACAAGAA CATATACGAG GGATGGTCTA TTGGAAGACC CGGATAATGT TAGCTTCCGT 
GAAGCTCTCT CTGAAGGCAA AGATATTGAA GTCGCCAAAG ATCTTCAAAG AGTTCACGAT 
CCACATGATG AAAGTGATGA AATGACGTCA GATGAGGTTG AATTACATGT TAATTTGGGC 
CAAGTTGGGT TCGTCTTTAT AGAAGCCAAT GTGAAAAAAT ATGCGTTTGG GAGTGTTTAT 
GGCCAAATTG GAATTCCTCC TGCGTATAAT GGAACCGAGA TCAAGAAGGA TACTATTTTA 
CAGAAAGGAG AAGAATTGCC ACCAAGATAT GCTGACACTG ATAATTTCTT TGGTAGTATG 
AAGGTAAAAG AAGGGTCATC CTCTAGGATA ACGGCGCAAA CTAGTAAGCC CCTGTGGTCT 
GTTGGGACGT ATGAAAGAAT CTCTTCTAAC TTTGACAGAG AAAATAATGT TTACCACGAC 
AGTCTTGAAA CCGACGATAA CAACACCGAT AACAATGTTA ATAACAACGA TGAGAACGCT 
GGTTGCAATG AAAATTCGCC ATTATTGGAA GATGATGGCA ATAAAAGACC GGAAAATTCA 
AATACCCCCC GTGAAGTATC AGATGGAGCT ATCAATAAGA ACCCTAGAAA TAAATCTACT 
AAAAAACGTC AAAGAAACAG AGGCAAATCT TCTAAAAAGA AGAACAGATC GAGAAAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Life with 6000 genes.
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)
Goffeau A,Barrell BG,Bussey H,Davis RW,Dujon B,Feldmann H,Galibert F,Hoheisel JD,Jacq C,Johnston M,Louis EJ,Mewes HW,Murakami Y,Philippsen P,Tettelin H,Oliver SG

Complete DNA sequence of yeast chromosome XI.
Nature369(6479)371-8(1994 Jun)
Dujon B,Alexandraki D,Andr? B,Ansorge W,Baladron V,Ballesta JP,Banrevi A,Bolle PA,Bolotin-Fukuhara M,Bossier P,Bou G,Boyer J,Bultrago MJ,Cheret G,Colleaux L,Dalgnan-Fornler B,del Rey F,Dlon C,Domdey H,D?sterhoft A,D?sterhus S,Entlan KD,Erfle H,Esteban PF,Feldmann H,Fernandes L,Robo GM,Fritz C,Fukuhara H,Gabel C,Gaillon L,Carcia-Cantalejo JM,Garcia-Ramirez JJ,Gent NE,Ghazvini M,Goffeau A,Gonzal?z A,Grothues D,Guerreiro P,Hegemann J,Hewitt N,Hilger F,Hollenberg CP,Horaitis O,Indge KJ,Jacquier A,James CM,Jauniaux C,Jimenez A,Keuchel H,Kirchrath L,Kleine K,K?tter P,Legrain P,Liebl S,Louis EJ,Maia e Silva A,Marck C,Monnier AL,M?stl D,M?ller S,Obermaier B,Oliver SG,Pallier C,Pascolo S,Pfeiffer F,Philippsen P,Planta RJ,Pohl FM,Pohl TM,P?hlmann R,Portetelle D,Purnelle B,Puzos V,Ramezani Rad M,Rasmussen SW,Remacha M,Revuelta JL,Richard GF,Rieger M,Rodrigues-Pousada C,Rose M,Rupp T,Santos MA,Schwager C,Sensen C,Skala J,Soares H,Sor F,Stegemann J,Tettelin H,Thierry A,Tzermia M,Urrestarazu LA,van Dyck L,Van Vliet-Reedijk JC,Valens M,Vandenbo M,Vilela C,Vissers S,von Wettstein D,Voss H,Wiemann S,Xu G,Zimmermann J,Haasemann M,Becker I,Mewes HW

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SSH4 cDNA ORF clone, Saccharomyces cerevisiae S288C

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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