MET5 cDNA ORF clone, Saccharomyces cerevisiae S288C

Gene
Clones
gRNAs

Summary

Gene Symbol	MET5
Entrez Gene ID	853602
Full Name	sulfite reductase (NADPH) subunit beta
Synonyms	ECM17
Gene Type	protein-coding
Organism	Saccharomyces cerevisiae S288C

mRNA and Protein(s)

mRNA	Protein	Name
NM_001181795.1	NP_116579.1	sulfite reductase (NADPH) subunit beta

Pathways from BioSystems

Homologies

Saccharomyces cerevisiae (baker's yeast)	MET5	NP_116579.1
Kluyveromyces lactis	KLLA0B03938g	XP_451709.1
Arabidopsis thaliana (thale cress)	SIR	NP_196079.1
Eremothecium gossypii	AGOS_ABL077W	NP_982870.2
Schizosaccharomyces pombe (fission yeast)	sir1	NP_593252.1
Magnaporthe oryzae (rice blast fungus)	MGG_04144	XP_003719686.1
Neurospora crassa	NCU05238	XP_962002.2
Oryza sativa (rice)	Os05g0503300	NP_001055978.1

Gene Ontology
Bibliography

Related articles in PubMed

Life with 6000 genes.
Goffeau A, Barrell BG, Bussey H, Davis RW, Dujon B, Feldmann H, Galibert F, Hoheisel JD, Jacq C, Johnston M, Louis EJ, Mewes HW, Murakami Y, Philippsen P, Tettelin H, Oliver SG
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)

Complete nucleotide sequence of Saccharomyces cerevisiae chromosome X.
Galibert F, Alexandraki D, Baur A, Boles E, Chalwatzis N, Chuat JC, Coster F, Cziepluch C, De Haan M, Domdey H, Durand P, Entian KD, Gatius M, Goffeau A, Grivell LA, Hennemann A, Herbert CJ, Heumann K, Hilger F, Hollenberg CP, Huang ME, Jacq C, Jauniaux JC, Katsoulou C, Karpfinger-Hartl L
The EMBO journal15(9)2031-49(1996 May)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Specific mutations in the MET10 and MET5 genes, which encode the catalytic alpha- and beta-subunits of the sulfite reductase enzyme, respectively, were identified in strains producing decreased amount of H2S.
Title: Isolation of sulfite reductase variants of a commercial wine yeast with significantly reduced hydrogen sulfide production.

The following MET5 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the MET5 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OSi02830	NM_001181795.1 Latest version!	Saccharomyces cerevisiae S288C sulfite reductase (NADPH) subunit beta (MET5), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

MET5 ( NM_001181795.1 ) cDNA ORF clone, Saccharomyces cerevisiae S288C -> NP_116579.1 Saccharomyces cerevisiae S288C sulfite reductase (NADPH) subunit beta (MET5), partial mRNA.

Price & Availability↑

CloneID	OSi02830
Clone ID Related Accession (Same CDS sequence)	NM_001181795.1
Accession Version	NM_001181795.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4329bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-31
Organism	Saccharomyces cerevisiae S288C
Product	sulfite reductase (NADPH) subunit beta
Comment	Comment: REVIEWED REFSEQ: This record has been curated by SGD. This record is derived from an annotated genomic sequence (NC_001142). ##Genome-Annotation-Data-START## Annotation Provider :: SGD Annotation Status :: Full Annotation Annotation Version :: R64-2-1 URL :: http://www.yeastgenome.org/ ##Genome-Annotation-Data-END## COMPLETENESS: incomplete on both ends.

ATGACTGCTT CTGACCTCTT GACGCTCCCA CAATTGTTGG CGCAATATTC CTCTAGTGCT 
CCCCAAAATA AAGTGTTCTA CACTACTAGC ACAAAAAATA GTCATTCATC CTTCAAAGGG 
CTAGAATCTG TTGCCACAGA TGCTACTCAT CTATTGAACA ATCAAGATCC ATTGAATACC 
ATCAAAGATC AACTCTCTAA GGACATTTTA ACTACCGTTT TTACAGATGA AACTACTTTG 
GTCAAATCCA TCCACCATCT ATATTCTCTT CCTAATAAAC TTCCATTGGT GATTACAGTG 
GATTTGAATT TGCAAGATTA TTCCGCAATT CCTGCGTTAA AGGATCTTTC TTTCCCCATC 
TTAATATCTT CTGATTTGCA AACTGCAATT TCAAACGCGG ACTCTTCTTA TAAGATTGCT 
ACAAGTTCTC TGACCCCAGT TTTCCATTTT CTTAACTTGG AGAAAATTGG CACGAGTACA 
GCTATTGAAC AAGACATCGA CTTTCCTACC TTGGAGATTG CTAACGAAGA GACTAAAGTA 
GCATTATCAG AAGCTACTGA TTCATTGACA AATTTTGAAC TGGTTAAAGG TAAAGAGTCA 
ATTACCACTG TCATTGTTAA CCTGTCACCA TATGACGCGG AATTCAGTAG CGTATTGCCT 
TCAAACGTAG GATTGATCAA GATTAGAGTA TACAGACCAT GGAATTTTTC CAAGTTCTTG 
GAAATACTGC CATCTTCTGT TACCAAAATC GCCGTTTTAC AGGGTGTCTC TAAGAAATCG 
CAATCAAACG AATTTCAACC ATTTCTTTTG GACTTCTTTG GCAATTTTAA TGAATTAGTT 
TCTAGGAACA TCGAGCAGGT AGTGTTGACT AATATTGGTA ATGTGAATGA TTATGGCAAC 
GTTATCAACA CCGTCATATC GAATATTAAC AAGAAGGAAC CAGATAATAA CTTATTTTTA 
GGTGAATCCA ATGAAAAGGC TGAGGAACAA GCTGAAGTTA CTCAACTTAT TTCTTCTGTC 
AAAAAAGTTG TGAACTTAGA AGACGCCTAT ATCAAAGTGC TAAAACAGTT ATTTTCATCA 
AATCTACAAA TTTTGAATCA ATTTTCCAGT GAGACAATTG AACCAAGTAA TCCAGAATTT 
GGTTTTGGAC GCTTTTTAAA ACAAGAAGCC CAGCGTGAAG AATTGATCAG CTTAGCAAAA 
ACCTCTCTTG ATCCAAGTCT TTACTTGTCC GAGGATGCAA ATAAAATTGT TCAACTATTA 
TCTAAATGGT TGTCATTCAA TGGACGCGAT CTTGACGAAG CTCAATTACA AGAGGCCAAT 
GCGACAGGTT TGGAAATATT TCAGTTATTA CAATCTAATC AAGATTCCTC TACTGTCTTA 
AAATTCTTAA AGATAGCTCC AACAAGCGAT TCTTTTATTT TCAAATCAAG CTGGCTAATT 
GGCTCCGATG CCTGGTCTTA TGATTTGGGT CACTCAGGTA TTCAACAGGT TTTATCCTCC 
CGTAAAAACA TTAATGTTTT ATTGATTGAT TCAGAGCCAT ATGACCATAG AAAGCAAAAC 
CAGGATAGAA AGAAAGATGT TGGTTTGTAC GCCATGAATT ATTACAGTGC CTATGTTGCC 
TCTGTAGCAG TATATGCTTC TTACACCCAA CTATTGACTG CAATAATAGA GGCATCTAAA 
TACAATGGTC CTTCTATTGT CTTGGCTTAT TTGCCGTATA ATTCCGAAAA TGATACTCCA 
TTAGAAGTCT TAAAGGAAAC CAAAAACGCC GTTGAATCTG GTTACTGGCC ATTGTATAGG 
TTTAATCCTG TTTATGACGA TCCATCAACA GATAAGGAGG CATTTAGCTT GGATTCTTCG 
GTTATCAGAA AACAATTACA GGACTTCTTA GACCGTGAGA ATAAGCTTAC CCTATTAACC 
AGAAAAGATC CATCTTTGTC AAGAAATCTA AAGCAATCTG CTGGTGATGC GTTGACAAGG 
AAACAAGAAA AAAGAAGCAA GGCTGCCTTC GATCAGTTAT TGGAGGGTTT GTCCGGCCCA 
CCGCTACACG TCTATTATGC TTCTGACGGT GGTAATGCTG CAAACTTGGC AAAGAGACTA 
GCAGCAAGGG CATCTGCAAG AGGTTTAAAG GCTACTGTTC TGTCCATGGA TGACATTATT 
TTGGAAGAAT TACCTGGTGA AGAGAATGTA GTTTTCATTA CTTCCACGGC TGGGCAAGGT 
GAATTCCCCC AAGACGGTAA GTCTTTCTGG GAAGCTCTGA AAAATGACAC CGACTTGGAT 
TTAGCTAGTT TGAATGTTGC TGTTTTTGGT CTCGGTGATT CTGAGTATTG GCCACGTAAA 
GAAGATAAAC ATTATTTTAA CAAGCCTTCA CAGGATTTAT TTAAGCGCTT GGAATTATTG 
AGTGCCAAAG CCCTAATTCC CTTGGGACTG GGTGATGATC AAGATGCTGA TGGTTTCCAA 
ACTGCTTATT CTGAGTGGGA ACCTAAATTA TGGGAAGCTC TTGGTGTTTC CGGCGCTGCT 
GTTGATGATG AGCCAAAACC TGTTACAAAC GAGGATATTA AGAGAGAATC TAATTTCTTG 
AGAGGTACTA TCAGTGAGAA CTTAAAGGAT ACTTCTTCAG GTGGTGTTAC TCACGCTAAT 
GAACAATTAA TGAAATTTCA CGGTATTTAC ACCCAAGACG ATCGTGACAT TAGAGAAATA 
CGTAAGTCAC AAGGCTTAGA GCCATACTAT ATGTTTATGG CAAGAGCTCG TTTACCAGGT 
GGTAAGACCA CTCCACAACA ATGGCTTGCT CTGGATCACT TATCTGATAC TTCAGGCAAT 
GGTACCCTGA AATTAACAAC AAGGGCAACC TTCCAGATTC ATGGTGTGCT AAAGAAGAAC 
TTGAAACACA CATTGAGAGG AATGAATGCA GTTCTTATGG ATACATTAGC TGCTGCAGGT 
GACGTGAACA GAAATGTCAT GGTTTCTGCT CTACCAACCA ATGCCAAGGT TCACCAACAA 
ATCGCTGATA TGGGAAAATT GATTTCTGAT CATTTCTTAC CAAAGACTAC GGCCTACCAC 
GAAGTTTGGC TGGAGGGCCC AGAAGAACAG GACGATGATC CATCCTGGCC ATCTATTTTT 
GAAAACAGAA AAGATGGTCC AAGAAAAAAG AAGACTCTAG TTAGCGGTAA CGCTTTGGTC 
GATATTGAAC CAATTTACGG TCCAACTTAT CTGCCAAGAA AGTTTAAATT CAACATCGCC 
GTTCCTCCAT ATAACGATGT GGATGTATTA TCTATCGATG TCGGTTTAGT TGCTATAGTT 
AACCCAGAAA CTCAAATCGT GGAGGGTTAT AATGTTTTTG TTGGTGGTGG TATGGGTACC 
ACTCATAACA ACAAGAAAAC TTACCCAAGA TTAGGGTCAT GCTTAGGTTT TGTTAAAACT 
GAAGACATTA TTCCACCACT TGAAGGTATC GTTATTGTCC AAAGAGATCA CGGTGACCGT 
AAAGACCGTA AGCATGCTCG TTTAAAGTAT ACTGTAGATG ATATGGGTGT CGAAGGCTTC 
AAGCAAAAAG TGGAGGAATA CTGGGGTAAG AAATTCGAGC CTGAGAGACC ATTTGAGTTT 
AAATCTAATA TTGATTACTT TGGATGGATT AAAGATGAAA CTGGGTTAAA CCACTTTACC 
GCATTTATTG AAAATGGTAG GGTTGAAGAT ACACCAGATT TGCCTCAAAA GACAGGTATT 
AGAAAGGTTG CTGAATACAT GCTTAAGACT AATTCTGGTC ATTTCAGATT GACTGGTAAT 
CAACATTTGG TTATCTCTAA TATTACAGAT GAACATGTTG CTGGAATAAA ATCTATTTTA 
AAGACCTATA AATTGGATAA CACCGATTTC AGCGGTTTGA GATTATCTTC ATCTTCCTGT 
GTTGGTTTGC CAACATGTGG TTTAGCGTTT GCCGAATCTG AACGTTTCCT ACCTGACATT 
ATTACTCAGT TGGAAGATTG TTTAGAAGAG TATGGTTTAC GCCATGATTC CATTATTATG 
AGAATGACTG GTTGCCCTAA CGGTTGTTCT CGTCCATGGC TAGGTGAATT AGCTCTTGTT 
GGTAAAGCTC CACATACTTA TAACTTGATG CTTGGTGGTG GTTACCTCGG CCAAAGGCTA 
AACAAATTGT ATAAGGCCAA TGTGAAGGAT GAGGAAATTG TCGACTACAT CAAACCATTG 
TTTAAAAGGT ATGCTTTAGA AAGAGAAGAA GGGGAACACT TTGGTGATTT CTGTATAAGA 
GTAGGTATCA TTAAACCAAC CACCGAGGGT AAATACTTCC ATGAAGATGT GTCTGAAGAT 
GCCTATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_116579.1
CDS	1..4329
Translation	MTASDLLTLPQLLAQYSSSAPQNKVFYTTSTKNSHSSFKGLESVATDATHLLNNQDPLNTIKDQLSKDILTTVFTDETTLVKSIHHLYSLPNKLPLVITVDLNLQDYSAIPALKDLSFPILISSDLQTAISNADSSYKIATSSLTPVFHFLNLEKIGTSTAIEQDIDFPTLEIANEETKVALSEATDSLTNFELVKGKESITTVIVNLSPYDAEFSSVLPSNVGLIKIRVYRPWNFSKFLEILPSSVTKIAVLQGVSKKSQSNEFQPFLLDFFGNFNELVSRNIEQVVLTNIGNVNDYGNVINTVISNINKKEPDNNLFLGESNEKAEEQAEVTQLISSVKKVVNLEDAYIKVLKQLFSSNLQILNQFSSETIEPSNPEFGFGRFLKQEAQREELISLAKTSLDPSLYLSEDANKIVQLLSKWLSFNGRDLDEAQLQEANATGLEIFQLLQSNQDSSTVLKFLKIAPTSDSFIFKSSWLIGSDAWSYDLGHSGIQQVLSSRKNINVLLIDSEPYDHRKQNQDRKKDVGLYAMNYYSAYVASVAVYASYTQLLTAIIEASKYNGPSIVLAYLPYNSENDTPLEVLKETKNAVESGYWPLYRFNPVYDDPSTDKEAFSLDSSVIRKQLQDFLDRENKLTLLTRKDPSLSRNLKQSAGDALTRKQEKRSKAAFDQLLEGLSGPPLHVYYASDGGNAANLAKRLAARASARGLKATVLSMDDIILEELPGEENVVFITSTAGQGEFPQDGKSFWEALKNDTDLDLASLNVAVFGLGDSEYWPRKEDKHYFNKPSQDLFKRLELLSAKALIPLGLGDDQDADGFQTAYSEWEPKLWEALGVSGAAVDDEPKPVTNEDIKRESNFLRGTISENLKDTSSGGVTHANEQLMKFHGIYTQDDRDIREIRKSQGLEPYYMFMARARLPGGKTTPQQWLALDHLSDTSGNGTLKLTTRATFQIHGVLKKNLKHTLRGMNAVLMDTLAAAGDVNRNVMVSALPTNAKVHQQIADMGKLISDHFLPKTTAYHEVWLEGPEEQDDDPSWPSIFENRKDGPRKKKTLVSGNALVDIEPIYGPTYLPRKFKFNIAVPPYNDVDVLSIDVGLVAIVNPETQIVEGYNVFVGGGMGTTHNNKKTYPRLGSCLGFVKTEDIIPPLEGIVIVQRDHGDRKDRKHARLKYTVDDMGVEGFKQKVEEYWGKKFEPERPFEFKSNIDYFGWIKDETGLNHFTAFIENGRVEDTPDLPQKTGIRKVAEYMLKTNSGHFRLTGNQHLVISNITDEHVAGIKSILKTYKLDNTDFSGLRLSSSSCVGLPTCGLAFAESERFLPDIITQLEDCLEEYGLRHDSIIMRMTGCPNGCSRPWLGELALVGKAPHTYNLMLGGGYLGQRLNKLYKANVKDEEIVDYIKPLFKRYALEREEGEHFGDFCIRVGIIKPTTEGKYFHEDVSEDAY

Target ORF information:

RefSeq Version	NM_001181795.1
Organism	Saccharomyces cerevisiae S288C
Definition	Saccharomyces cerevisiae S288C sulfite reductase (NADPH) subunit beta (MET5), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001181795.1

ORF Insert Sequence:

ATGACTGCTT CTGACCTCTT GACGCTCCCA CAATTGTTGG CGCAATATTC CTCTAGTGCT 
CCCCAAAATA AAGTGTTCTA CACTACTAGC ACAAAAAATA GTCATTCATC CTTCAAAGGG 
CTAGAATCTG TTGCCACAGA TGCTACTCAT CTATTGAACA ATCAAGATCC ATTGAATACC 
ATCAAAGATC AACTCTCTAA GGACATTTTA ACTACCGTTT TTACAGATGA AACTACTTTG 
GTCAAATCCA TCCACCATCT ATATTCTCTT CCTAATAAAC TTCCATTGGT GATTACAGTG 
GATTTGAATT TGCAAGATTA TTCCGCAATT CCTGCGTTAA AGGATCTTTC TTTCCCCATC 
TTAATATCTT CTGATTTGCA AACTGCAATT TCAAACGCGG ACTCTTCTTA TAAGATTGCT 
ACAAGTTCTC TGACCCCAGT TTTCCATTTT CTTAACTTGG AGAAAATTGG CACGAGTACA 
GCTATTGAAC AAGACATCGA CTTTCCTACC TTGGAGATTG CTAACGAAGA GACTAAAGTA 
GCATTATCAG AAGCTACTGA TTCATTGACA AATTTTGAAC TGGTTAAAGG TAAAGAGTCA 
ATTACCACTG TCATTGTTAA CCTGTCACCA TATGACGCGG AATTCAGTAG CGTATTGCCT 
TCAAACGTAG GATTGATCAA GATTAGAGTA TACAGACCAT GGAATTTTTC CAAGTTCTTG 
GAAATACTGC CATCTTCTGT TACCAAAATC GCCGTTTTAC AGGGTGTCTC TAAGAAATCG 
CAATCAAACG AATTTCAACC ATTTCTTTTG GACTTCTTTG GCAATTTTAA TGAATTAGTT 
TCTAGGAACA TCGAGCAGGT AGTGTTGACT AATATTGGTA ATGTGAATGA TTATGGCAAC 
GTTATCAACA CCGTCATATC GAATATTAAC AAGAAGGAAC CAGATAATAA CTTATTTTTA 
GGTGAATCCA ATGAAAAGGC TGAGGAACAA GCTGAAGTTA CTCAACTTAT TTCTTCTGTC 
AAAAAAGTTG TGAACTTAGA AGACGCCTAT ATCAAAGTGC TAAAACAGTT ATTTTCATCA 
AATCTACAAA TTTTGAATCA ATTTTCCAGT GAGACAATTG AACCAAGTAA TCCAGAATTT 
GGTTTTGGAC GCTTTTTAAA ACAAGAAGCC CAGCGTGAAG AATTGATCAG CTTAGCAAAA 
ACCTCTCTTG ATCCAAGTCT TTACTTGTCC GAGGATGCAA ATAAAATTGT TCAACTATTA 
TCTAAATGGT TGTCATTCAA TGGACGCGAT CTTGACGAAG CTCAATTACA AGAGGCCAAT 
GCGACAGGTT TGGAAATATT TCAGTTATTA CAATCTAATC AAGATTCCTC TACTGTCTTA 
AAATTCTTAA AGATAGCTCC AACAAGCGAT TCTTTTATTT TCAAATCAAG CTGGCTAATT 
GGCTCCGATG CCTGGTCTTA TGATTTGGGT CACTCAGGTA TTCAACAGGT TTTATCCTCC 
CGTAAAAACA TTAATGTTTT ATTGATTGAT TCAGAGCCAT ATGACCATAG AAAGCAAAAC 
CAGGATAGAA AGAAAGATGT TGGTTTGTAC GCCATGAATT ATTACAGTGC CTATGTTGCC 
TCTGTAGCAG TATATGCTTC TTACACCCAA CTATTGACTG CAATAATAGA GGCATCTAAA 
TACAATGGTC CTTCTATTGT CTTGGCTTAT TTGCCGTATA ATTCCGAAAA TGATACTCCA 
TTAGAAGTCT TAAAGGAAAC CAAAAACGCC GTTGAATCTG GTTACTGGCC ATTGTATAGG 
TTTAATCCTG TTTATGACGA TCCATCAACA GATAAGGAGG CATTTAGCTT GGATTCTTCG 
GTTATCAGAA AACAATTACA GGACTTCTTA GACCGTGAGA ATAAGCTTAC CCTATTAACC 
AGAAAAGATC CATCTTTGTC AAGAAATCTA AAGCAATCTG CTGGTGATGC GTTGACAAGG 
AAACAAGAAA AAAGAAGCAA GGCTGCCTTC GATCAGTTAT TGGAGGGTTT GTCCGGCCCA 
CCGCTACACG TCTATTATGC TTCTGACGGT GGTAATGCTG CAAACTTGGC AAAGAGACTA 
GCAGCAAGGG CATCTGCAAG AGGTTTAAAG GCTACTGTTC TGTCCATGGA TGACATTATT 
TTGGAAGAAT TACCTGGTGA AGAGAATGTA GTTTTCATTA CTTCCACGGC TGGGCAAGGT 
GAATTCCCCC AAGACGGTAA GTCTTTCTGG GAAGCTCTGA AAAATGACAC CGACTTGGAT 
TTAGCTAGTT TGAATGTTGC TGTTTTTGGT CTCGGTGATT CTGAGTATTG GCCACGTAAA 
GAAGATAAAC ATTATTTTAA CAAGCCTTCA CAGGATTTAT TTAAGCGCTT GGAATTATTG 
AGTGCCAAAG CCCTAATTCC CTTGGGACTG GGTGATGATC AAGATGCTGA TGGTTTCCAA 
ACTGCTTATT CTGAGTGGGA ACCTAAATTA TGGGAAGCTC TTGGTGTTTC CGGCGCTGCT 
GTTGATGATG AGCCAAAACC TGTTACAAAC GAGGATATTA AGAGAGAATC TAATTTCTTG 
AGAGGTACTA TCAGTGAGAA CTTAAAGGAT ACTTCTTCAG GTGGTGTTAC TCACGCTAAT 
GAACAATTAA TGAAATTTCA CGGTATTTAC ACCCAAGACG ATCGTGACAT TAGAGAAATA 
CGTAAGTCAC AAGGCTTAGA GCCATACTAT ATGTTTATGG CAAGAGCTCG TTTACCAGGT 
GGTAAGACCA CTCCACAACA ATGGCTTGCT CTGGATCACT TATCTGATAC TTCAGGCAAT 
GGTACCCTGA AATTAACAAC AAGGGCAACC TTCCAGATTC ATGGTGTGCT AAAGAAGAAC 
TTGAAACACA CATTGAGAGG AATGAATGCA GTTCTTATGG ATACATTAGC TGCTGCAGGT 
GACGTGAACA GAAATGTCAT GGTTTCTGCT CTACCAACCA ATGCCAAGGT TCACCAACAA 
ATCGCTGATA TGGGAAAATT GATTTCTGAT CATTTCTTAC CAAAGACTAC GGCCTACCAC 
GAAGTTTGGC TGGAGGGCCC AGAAGAACAG GACGATGATC CATCCTGGCC ATCTATTTTT 
GAAAACAGAA AAGATGGTCC AAGAAAAAAG AAGACTCTAG TTAGCGGTAA CGCTTTGGTC 
GATATTGAAC CAATTTACGG TCCAACTTAT CTGCCAAGAA AGTTTAAATT CAACATCGCC 
GTTCCTCCAT ATAACGATGT GGATGTATTA TCTATCGATG TCGGTTTAGT TGCTATAGTT 
AACCCAGAAA CTCAAATCGT GGAGGGTTAT AATGTTTTTG TTGGTGGTGG TATGGGTACC 
ACTCATAACA ACAAGAAAAC TTACCCAAGA TTAGGGTCAT GCTTAGGTTT TGTTAAAACT 
GAAGACATTA TTCCACCACT TGAAGGTATC GTTATTGTCC AAAGAGATCA CGGTGACCGT 
AAAGACCGTA AGCATGCTCG TTTAAAGTAT ACTGTAGATG ATATGGGTGT CGAAGGCTTC 
AAGCAAAAAG TGGAGGAATA CTGGGGTAAG AAATTCGAGC CTGAGAGACC ATTTGAGTTT 
AAATCTAATA TTGATTACTT TGGATGGATT AAAGATGAAA CTGGGTTAAA CCACTTTACC 
GCATTTATTG AAAATGGTAG GGTTGAAGAT ACACCAGATT TGCCTCAAAA GACAGGTATT 
AGAAAGGTTG CTGAATACAT GCTTAAGACT AATTCTGGTC ATTTCAGATT GACTGGTAAT 
CAACATTTGG TTATCTCTAA TATTACAGAT GAACATGTTG CTGGAATAAA ATCTATTTTA 
AAGACCTATA AATTGGATAA CACCGATTTC AGCGGTTTGA GATTATCTTC ATCTTCCTGT 
GTTGGTTTGC CAACATGTGG TTTAGCGTTT GCCGAATCTG AACGTTTCCT ACCTGACATT 
ATTACTCAGT TGGAAGATTG TTTAGAAGAG TATGGTTTAC GCCATGATTC CATTATTATG 
AGAATGACTG GTTGCCCTAA CGGTTGTTCT CGTCCATGGC TAGGTGAATT AGCTCTTGTT 
GGTAAAGCTC CACATACTTA TAACTTGATG CTTGGTGGTG GTTACCTCGG CCAAAGGCTA 
AACAAATTGT ATAAGGCCAA TGTGAAGGAT GAGGAAATTG TCGACTACAT CAAACCATTG 
TTTAAAAGGT ATGCTTTAGA AAGAGAAGAA GGGGAACACT TTGGTGATTT CTGTATAAGA 
GTAGGTATCA TTAAACCAAC CACCGAGGGT AAATACTTCC ATGAAGATGT GTCTGAAGAT 
GCCTATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Life with 6000 genes.
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)
Goffeau A,Barrell BG,Bussey H,Davis RW,Dujon B,Feldmann H,Galibert F,Hoheisel JD,Jacq C,Johnston M,Louis EJ,Mewes HW,Murakami Y,Philippsen P,Tettelin H,Oliver SG

Complete nucleotide sequence of Saccharomyces cerevisiae chromosome X.
The EMBO journal15(9)2031-49(1996 May)
Galibert F,Alexandraki D,Baur A,Boles E,Chalwatzis N,Chuat JC,Coster F,Cziepluch C,De Haan M,Domdey H,Durand P,Entian KD,Gatius M,Goffeau A,Grivell LA,Hennemann A,Herbert CJ,Heumann K,Hilger F,Hollenberg CP,Huang ME,Jacq C,Jauniaux JC,Katsoulou C,Karpfinger-Hartl L

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MET5 cDNA ORF clone, Saccharomyces cerevisiae S288C

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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