OST1 cDNA ORF clone, Saccharomyces cerevisiae S288C

Gene
Clones
gRNAs

Summary

Gene Symbol	OST1
Entrez Gene ID	853455
Full Name	dolichyl-diphosphooligosaccharide--protein glycotransferase subunit OST1
Synonyms	NLT1
Gene Type	protein-coding
Organism	Saccharomyces cerevisiae S288C

mRNA and Protein(s)

mRNA	Protein	Name
NM_001181436.3	NP_012532.3	dolichyl-diphosphooligosaccharide--protein glycotransferase subunit OST1

Pathways from BioSystems

Homologies

Schizosaccharomyces pombe (fission yeast)	ost1	NP_594536.1
Saccharomyces cerevisiae (baker's yeast)	OST1	NP_012532.3
Eremothecium gossypii	AGOS_AAL170W	NP_982372.1
Magnaporthe oryzae (rice blast fungus)	MGG_09287	XP_003709936.1
Arabidopsis thaliana (thale cress)	AT2G01720	NP_178281.1
Kluyveromyces lactis	KLLA0F12980g	XP_455664.1
Neurospora crassa	NCU02541	XP_965672.1

Gene Ontology
Bibliography

Related articles in PubMed

Life with 6000 genes.
Goffeau A, Barrell BG, Bussey H, Davis RW, Dujon B, Feldmann H, Galibert F, Hoheisel JD, Jacq C, Johnston M, Louis EJ, Mewes HW, Murakami Y, Philippsen P, Tettelin H, Oliver SG
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)

Complete nucleotide sequence of Saccharomyces cerevisiae chromosome X.
Galibert F, Alexandraki D, Baur A, Boles E, Chalwatzis N, Chuat JC, Coster F, Cziepluch C, De Haan M, Domdey H, Durand P, Entian KD, Gatius M, Goffeau A, Grivell LA, Hennemann A, Herbert CJ, Heumann K, Hilger F, Hollenberg CP, Huang ME, Jacq C, Jauniaux JC, Katsoulou C, Karpfinger-Hartl L
The EMBO journal15(9)2031-49(1996 May)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Nup159 and Gle1-IP(6) regulate Dbp5 cycles by controlling its nucleotide-bound state, allowing multiple cycles of mRNP remodeling by a single Dbp5 at the nuclear pore complexes
Title: The Dbp5 cycle at the nuclear pore complex during mRNA export II: nucleotide cycling and mRNP remodeling by Dbp5 are controlled by Nup159 and Gle1.

The Dbp5-Gle1 interaction is limiting for export and, importantly, can be independent of Nup159.
Title: The Dbp5 cycle at the nuclear pore complex during mRNA export I: dbp5 mutants with defects in RNA binding and ATP hydrolysis define key steps for Nup159 and Gle1.

We report a 12 A resolution cryo-electron microscopy structure of OT from yeast.
Title: Structure of the oligosaccharyl transferase complex at 12 A resolution.

The following OST1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the OST1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OSi02700	NM_001181436.3 Latest version!	Saccharomyces cerevisiae S288C dolichyl-diphosphooligosaccharide--protein glycotransferase subunit OST1 (OST1), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

OST1 ( NM_001181436.3 ) cDNA ORF clone, Saccharomyces cerevisiae S288C -> NP_012532.3 Saccharomyces cerevisiae S288C dolichyl-diphosphooligosaccharide--protein glycotransferase subunit OST1 (OST1), partial mRNA.

Price & Availability↑

CloneID	OSi02700
Clone ID Related Accession (Same CDS sequence)	NM_001181436.3
Accession Version	NM_001181436.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1431bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-31
Organism	Saccharomyces cerevisiae S288C
Product	dolichyl-diphosphooligosaccharide--protein glycotransferase subunit OST1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by SGD. This record is derived from an annotated genomic sequence (NC_001142). On Jul 30, 2012 this sequence version replaced NM_001181436.2. ##Genome-Annotation-Data-START## Annotation Provider :: SGD Annotation Status :: Full Annotation Annotation Version :: R64-2-1 URL :: http://www.yeastgenome.org/ ##Genome-Annotation-Data-END## COMPLETENESS: incomplete on both ends.

ATGAGGCAGG TTTGGTTCTC TTGGATTGTG GGATTGTTCC TATGTTTTTT CAACGTGTCT 
TCTGCTGCCC AATACGAGCC ACCTGCGACT TGGGAGAATG TTGATTATAA GAGGACAATA 
GACGTGTCAA ACGCTTATAT TTCAGAAACA ATCGAAATAA CTATCAAAAA CATAGCAAGC 
GAACCTGCGA CTGAATACTT CACAGCCTTT GAGAGTGGCA TCTTCAGTAA AGTTTCCTTT 
TTTTCAGCCT ATTTTACCAA CGAGGCAACT TTTTTAAATA GCCAATTACT TGCCAATTCG 
ACTACAGCAC CTGGTGACGA TGGTGAAAGT GAAATTAGAT ACGGGATCAT TCAATTTCCA 
AATGCAATTT CCCCTCAGGA AGAAGTTTCT TTAGTGATTA AGAGCTTCTA TAATACCGTA 
GGTATTCCTT ATCCTGAGCA CGTTGGAATG TCAGAAGAAC AACACCTATT GTGGGAAACG 
AACAGATTGC CGCTTTCTGC TTACGATACC AAGAAGGCCT CTTTTACGCT GATTGGTAGC 
TCATCATTTG AGGAGTACCA CCCCCCAAAT GACGAGAGTT TACTGGGAAA AGCTAATGGA 
AACTCTTTTG AGTTTGGGCC TTGGGAAGAT ATTCCGAGAT TTTCTTCAAA CGAAACCTTA 
GCAATTGTTT ATTCCCACAA TGCCCCATTG AATCAGGTAG TGAATTTGAG AAGAGATATT 
TGGCTTTCTC ATTGGGCTTC CACAATACAA TTTGAGGAAT ATTATGAATT AACAAACAAA 
GCCGCAAAAC TGTCTAAAGG ATTTTCAAGA TTAGAATTAA TGAAACAGAT TCAAACTCAG 
AATATGAGAC AAACTCACTT TGTTACTGTC TTAGACATGC TCCTGCCTGA GGGAGCTACT 
GATCATTATT TCACTGATTT GGTTGGCCTT GTTTCCACGT CGCATGCAGA ACGTGACCAT 
TTCTTTATAA GACCAAGATT CCCAATCTTT GGAGGTTGGA ACTACAATTT TACTGTCGGT 
TGGACTAATA AATTGTCCGA TTTCTTGCAT GTATCCTCTG GCTCAGACGA GAAATTCGTT 
GCTTCTATCC CAATTCTAAA CGGCCCACCG GACACTGTAT ATGATAATGT TGAATTATCG 
GTATTTCTTC CGGAAGGGGC CGAAATATTC GATATTGATT CTCCAGTCCC TTTTACAAAT 
GTTTCTATAG AAACCCAGAA ATCATACTTT GACCTAAATA AAGGTCACGT TAAATTAACT 
TTCAGTTACA GAAATTTGAT TAGTCAAGTT GCCAATGGCC AAGTCTTGAT AAAGTACGAC 
TACCCGAAAA GCTCTTTTTT CAAGAAGCCT CTGTCTATTG CTTGCTATAT TTTCACCGCA 
CTAATGGGAG TTTTTGTCTT AAAAACTTTG AACATGAACG TAACTAACTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_012532.3
CDS	1..1431
Translation	MRQVWFSWIVGLFLCFFNVSSAAQYEPPATWENVDYKRTIDVSNAYISETIEITIKNIASEPATEYFTAFESGIFSKVSFFSAYFTNEATFLNSQLLANSTTAPGDDGESEIRYGIIQFPNAISPQEEVSLVIKSFYNTVGIPYPEHVGMSEEQHLLWETNRLPLSAYDTKKASFTLIGSSSFEEYHPPNDESLLGKANGNSFEFGPWEDIPRFSSNETLAIVYSHNAPLNQVVNLRRDIWLSHWASTIQFEEYYELTNKAAKLSKGFSRLELMKQIQTQNMRQTHFVTVLDMLLPEGATDHYFTDLVGLVSTSHAERDHFFIRPRFPIFGGWNYNFTVGWTNKLSDFLHVSSGSDEKFVASIPILNGPPDTVYDNVELSVFLPEGAEIFDIDSPVPFTNVSIETQKSYFDLNKGHVKLTFSYRNLISQVANGQVLIKYDYPKSSFFKKPLSIACYIFTALMGVFVLKTLNMNVTN

Target ORF information:

RefSeq Version	NM_001181436.3
Organism	Saccharomyces cerevisiae S288C
Definition	Saccharomyces cerevisiae S288C dolichyl-diphosphooligosaccharide--protein glycotransferase subunit OST1 (OST1), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001181436.3

ORF Insert Sequence:

ATGAGGCAGG TTTGGTTCTC TTGGATTGTG GGATTGTTCC TATGTTTTTT CAACGTGTCT 
TCTGCTGCCC AATACGAGCC ACCTGCGACT TGGGAGAATG TTGATTATAA GAGGACAATA 
GACGTGTCAA ACGCTTATAT TTCAGAAACA ATCGAAATAA CTATCAAAAA CATAGCAAGC 
GAACCTGCGA CTGAATACTT CACAGCCTTT GAGAGTGGCA TCTTCAGTAA AGTTTCCTTT 
TTTTCAGCCT ATTTTACCAA CGAGGCAACT TTTTTAAATA GCCAATTACT TGCCAATTCG 
ACTACAGCAC CTGGTGACGA TGGTGAAAGT GAAATTAGAT ACGGGATCAT TCAATTTCCA 
AATGCAATTT CCCCTCAGGA AGAAGTTTCT TTAGTGATTA AGAGCTTCTA TAATACCGTA 
GGTATTCCTT ATCCTGAGCA CGTTGGAATG TCAGAAGAAC AACACCTATT GTGGGAAACG 
AACAGATTGC CGCTTTCTGC TTACGATACC AAGAAGGCCT CTTTTACGCT GATTGGTAGC 
TCATCATTTG AGGAGTACCA CCCCCCAAAT GACGAGAGTT TACTGGGAAA AGCTAATGGA 
AACTCTTTTG AGTTTGGGCC TTGGGAAGAT ATTCCGAGAT TTTCTTCAAA CGAAACCTTA 
GCAATTGTTT ATTCCCACAA TGCCCCATTG AATCAGGTAG TGAATTTGAG AAGAGATATT 
TGGCTTTCTC ATTGGGCTTC CACAATACAA TTTGAGGAAT ATTATGAATT AACAAACAAA 
GCCGCAAAAC TGTCTAAAGG ATTTTCAAGA TTAGAATTAA TGAAACAGAT TCAAACTCAG 
AATATGAGAC AAACTCACTT TGTTACTGTC TTAGACATGC TCCTGCCTGA GGGAGCTACT 
GATCATTATT TCACTGATTT GGTTGGCCTT GTTTCCACGT CGCATGCAGA ACGTGACCAT 
TTCTTTATAA GACCAAGATT CCCAATCTTT GGAGGTTGGA ACTACAATTT TACTGTCGGT 
TGGACTAATA AATTGTCCGA TTTCTTGCAT GTATCCTCTG GCTCAGACGA GAAATTCGTT 
GCTTCTATCC CAATTCTAAA CGGCCCACCG GACACTGTAT ATGATAATGT TGAATTATCG 
GTATTTCTTC CGGAAGGGGC CGAAATATTC GATATTGATT CTCCAGTCCC TTTTACAAAT 
GTTTCTATAG AAACCCAGAA ATCATACTTT GACCTAAATA AAGGTCACGT TAAATTAACT 
TTCAGTTACA GAAATTTGAT TAGTCAAGTT GCCAATGGCC AAGTCTTGAT AAAGTACGAC 
TACCCGAAAA GCTCTTTTTT CAAGAAGCCT CTGTCTATTG CTTGCTATAT TTTCACCGCA 
CTAATGGGAG TTTTTGTCTT AAAAACTTTG AACATGAACG TAACTAACTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Life with 6000 genes.
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)
Goffeau A,Barrell BG,Bussey H,Davis RW,Dujon B,Feldmann H,Galibert F,Hoheisel JD,Jacq C,Johnston M,Louis EJ,Mewes HW,Murakami Y,Philippsen P,Tettelin H,Oliver SG

Complete nucleotide sequence of Saccharomyces cerevisiae chromosome X.
The EMBO journal15(9)2031-49(1996 May)
Galibert F,Alexandraki D,Baur A,Boles E,Chalwatzis N,Chuat JC,Coster F,Cziepluch C,De Haan M,Domdey H,Durand P,Entian KD,Gatius M,Goffeau A,Grivell LA,Hennemann A,Herbert CJ,Heumann K,Hilger F,Hollenberg CP,Huang ME,Jacq C,Jauniaux JC,Katsoulou C,Karpfinger-Hartl L

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OST1 cDNA ORF clone, Saccharomyces cerevisiae S288C

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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