DPB11 cDNA ORF clone, Saccharomyces cerevisiae S288C

Gene
Clones
gRNAs

Summary

Gene Symbol	DPB11
Entrez Gene ID	853355
Full Name	protein kinase activating protein DPB11
Gene Type	protein-coding
Organism	Saccharomyces cerevisiae S288C

mRNA and Protein(s)

mRNA	Protein	Name
NM_001181523.1	NP_012445.1	protein kinase activating protein DPB11

Pathways from BioSystems

Homologies

Saccharomyces cerevisiae (baker's yeast)	DPB11	NP_012445.1
Kluyveromyces lactis	KLLA0F14300g	XP_455722.1
Eremothecium gossypii	AGOS_AFR095C	NP_985642.2

Gene Ontology
Bibliography

Related articles in PubMed

Life with 6000 genes.
Goffeau A, Barrell BG, Bussey H, Davis RW, Dujon B, Feldmann H, Galibert F, Hoheisel JD, Jacq C, Johnston M, Louis EJ, Mewes HW, Murakami Y, Philippsen P, Tettelin H, Oliver SG
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)

Complete nucleotide sequence of Saccharomyces cerevisiae chromosome X.
Galibert F, Alexandraki D, Baur A, Boles E, Chalwatzis N, Chuat JC, Coster F, Cziepluch C, De Haan M, Domdey H, Durand P, Entian KD, Gatius M, Goffeau A, Grivell LA, Hennemann A, Herbert CJ, Heumann K, Hilger F, Hollenberg CP, Huang ME, Jacq C, Jauniaux JC, Katsoulou C, Karpfinger-Hartl L
The EMBO journal15(9)2031-49(1996 May)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Budding yeast Fun30 and human SMARCAD1 are cell cycle-regulated by interaction with the DNA double strand break-localized scaffold protein Dpb11/TOPBP1, respectively. In yeast, this protein assembly additionally comprises the 9-1-1 damage sensor, is involved in localizing Fun30 to damaged chromatin, and thus is required for efficient long-range resection of DNA double strand breaks.
Title: Targeting of the Fun30 nucleosome remodeller by the Dpb11 scaffold facilitates cell cycle-regulated DNA end resection.

Upon extrusion of ssDNA from the central channel of Mcm2-7, Dpb11 dissociates from Mcm2-7, and Dpb11 binds to ssDNA, thereby allowing GINS to bind to Cdc45.Mcm2-7
Title: Dpb11 protein helps control assembly of the Cdc45?Mcm2-7?GINS replication fork helicase.

The Slx4-Dpb11 scaffold complex coordinates the response to replication fork stalling in S-phase and the subsequent mitosis.
Title: The Slx4-Dpb11 scaffold complex: coordinating the response to replication fork stalling in S-phase and the subsequent mitosis.

The ddc2-S4 mutation decreases phosphorylation of histone H2A more significantly than the absence of Ddc1 and Dpb11 function does.
Title: Ddc2 mediates Mec1 activation through a Ddc1- or Dpb11-independent mechanism.

Depletion of TopBP1/Dpb11 led to an accumulation of chromatin bridges.
Title: TopBP1/Dpb11 binds DNA anaphase bridges to prevent genome instability.

The following DPB11 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the DPB11 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OSi02623	NM_001181523.1 Latest version!	Saccharomyces cerevisiae S288C protein kinase activating protein DPB11 (DPB11), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

DPB11 ( NM_001181523.1 ) cDNA ORF clone, Saccharomyces cerevisiae S288C -> NP_012445.1 Saccharomyces cerevisiae S288C protein kinase activating protein DPB11 (DPB11), partial mRNA.

Price & Availability↑

CloneID	OSi02623
Clone ID Related Accession (Same CDS sequence)	NM_001181523.1
Accession Version	NM_001181523.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2295bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-31
Organism	Saccharomyces cerevisiae S288C
Product	protein kinase activating protein DPB11
Comment	Comment: REVIEWED REFSEQ: This record has been curated by SGD. This record is derived from an annotated genomic sequence (NC_001142). ##Genome-Annotation-Data-START## Annotation Provider :: SGD Annotation Status :: Full Annotation Annotation Version :: R64-2-1 URL :: http://www.yeastgenome.org/ ##Genome-Annotation-Data-END## COMPLETENESS: incomplete on both ends.

ATGAAGCCCT TTCAAGGAAT AACATTTTGT CCGACTGCAA TAAATAATGA AATCTTAGCT 
AAAAAAATAT CAAAGAAAAT TATTAAGTTA GGAGGTATAT TTTCTAAAGA TTTGACCAGA 
CAAGTTAATG TCCTTGTTGT TGGGTCCACC ACCAATACAA ACAAATTTAA ATTTGCGGTG 
AAGCATAGAT TCGATATCAT ATTTATTGAC ATTCAAGCCA TTGATGACAT TTATCAGCTC 
TGGCTGTCTG GAGAAAATAT ATTGCCGGAC TCTAATACGG CAACAATGAC AGGATCCACC 
TATGAGATGC TGAAGATACT TTACCGGCGA TTTTCGTTTA AATATTTGCA TAACTTCAAT 
ATCTTCATTG GCAGAATAAC CGACACTAAC ATAACTTCCA TCGATTCATT GGTTAGGAGT 
ATTAAAAAGT TAGGGTGTTC TAGTTATAAC TATCAAAACT TTGTAATCAA AGATACCTCT 
TCACACAATG ATGACGATGA TCAAGGTCAG AATGGGCAGA TATCCATTTT TGTTACGGAT 
ACTTTACTAG GAGCAAGAGT TAACGCTGCT ATTGAACAAA ATATACCTAT TGTACATTTC 
AAATGGATTC TCGATTGCCA AAAAAGAAGC GCACTGTTAC CGTACGATCC CTATTATCTT 
TTACCAAATA TTAAAGACCT GCCCTATGAT TCAATCGGAT CAAACTCTTG CGATTGCTGG 
GATAAAATAA ACACCACCTT TCCAACCAAT ATCGATGCCC AATCCTCTCT GCAGCGGCAG 
CAATCCTCCA GTACCCTGAC TCCTTCGCTC CCGAAAACAT CATCCCTCTT GAATAAATTT 
AAACCAAAGG GTGAAAAAAT ATGGGACAAG GCAATGTCTC TACAGCAGCA TAGTAAGACA 
AATTTTTCAG TACTAGGGCA GTCACCATTA TCGATAAATA ACAAACAAGA AGATTTAAGT 
GATAATAGTA CTTTGATTTT CAAAAATTGT GCGTTTATCA TTCATCATAT TTTTCCTGGC 
AATCACCGTT CAATACTAAC CAAAATTGTT GTTCAAAATG GGGGTAAAAT TGAGACGTCT 
TACTTGAGCG GCATTTACGA TCATTCTTAT TACATCATTC CAAGTAATAA AGCTCTTGAC 
TCCTTCAATG ATTTACCTGA GATTATAGAT GATAATGATG GTATAGTTAC AGAATTTTTC 
ATTGAACGCT GCTTGTATTA TCAAAAATTA CTACACCCAA TAGATTTATG GTCAAAACCC 
TTCCTCAGCA CAATAGAGTT TCAAGTTTCG TCTTCTTCAA AGTTATTGCA TCATGAATTT 
TCTTCTTCCC CTTTTCTGAA TGTTACTATC ACTGGATTCT CTGGCGTAGA GCTGTTACAT 
CTGACTAAAG TATTAAATCT TCTAAAACCA ATGGGCATCA ATTATGTAGA ATACCTCAAT 
AAATCCACTG ACATTCTGCT AATCAACTTA GCAGCTTTAC CCAGTATCCC GAAAACCCAT 
CCGTTATGGT CGAATGAATT TAGCGATCTT TTTACTCAGT TTTGCATTAA TAACAATAAT 
GATGATCCTG GTGATAATAA CAGAAAAGAT TTTCAAAATA ATTCAATCTT GAGAAATTCG 
ATGAAAAGGA AAATTGAATA TATCAAGAAA TTCCACTCCA TACCGGTAGT TACTCCAGCA 
TTTATTTTTA AATTATTGTC CGCTGCATCT GGAGAAAATA ATGAAATCTT TTTAAACAAT 
ATCAAGTGGT GTATTATCTG CCCAAGAGGA CACAAGGACG ATTTTAAATG TAAGATAAAA 
AAACCATACT ATACCAGCAT TAGTTCAGAA AAAAAGTACC AAAACAATGA TCCAAAAATC 
GACAAAACTA TTCTTTTGAA AAGAAACAAT TCCTCATTAT CGGAGCACTC TATGAAAGAT 
ACCAAAAACG AATTATTGCA GAAAATTAGA GAAACTGATT CTGGAAGAAA AAAGCGTAGT 
GTCTCATCGA GTATCATGGA TGTTTCTTCA GAGAGACAAA TGCCGGATAC GAAAAGGATC 
AAGTTGGAGT CACTGCCAAA AAATTTCGTT CCTAAACAAA TTAAACGAAC CACGAGTTGG 
GGCACAATAA TGTCAGAAAA TGTGCCTACA GAGCAGCCGA CTGCAATTTC TAATCCAGAA 
GAGATCCCAA GAACTGAGGA AGTTTCACAT ACTCAAGTTA CCTATGGCTC CATTCAAGAT 
AAGAAACGTA CTGCCTCTTT AGAAAAACCT ATGAGACGAC AGACAAGAAA TCAGACAAAG 
GAATTAGATT CTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_012445.1
CDS	1..2295
Translation	MKPFQGITFCPTAINNEILAKKISKKIIKLGGIFSKDLTRQVNVLVVGSTTNTNKFKFAVKHRFDIIFIDIQAIDDIYQLWLSGENILPDSNTATMTGSTYEMLKILYRRFSFKYLHNFNIFIGRITDTNITSIDSLVRSIKKLGCSSYNYQNFVIKDTSSHNDDDDQGQNGQISIFVTDTLLGARVNAAIEQNIPIVHFKWILDCQKRSALLPYDPYYLLPNIKDLPYDSIGSNSCDCWDKINTTFPTNIDAQSSLQRQQSSSTLTPSLPKTSSLLNKFKPKGEKIWDKAMSLQQHSKTNFSVLGQSPLSINNKQEDLSDNSTLIFKNCAFIIHHIFPGNHRSILTKIVVQNGGKIETSYLSGIYDHSYYIIPSNKALDSFNDLPEIIDDNDGIVTEFFIERCLYYQKLLHPIDLWSKPFLSTIEFQVSSSSKLLHHEFSSSPFLNVTITGFSGVELLHLTKVLNLLKPMGINYVEYLNKSTDILLINLAALPSIPKTHPLWSNEFSDLFTQFCINNNNDDPGDNNRKDFQNNSILRNSMKRKIEYIKKFHSIPVVTPAFIFKLLSAASGENNEIFLNNIKWCIICPRGHKDDFKCKIKKPYYTSISSEKKYQNNDPKIDKTILLKRNNSSLSEHSMKDTKNELLQKIRETDSGRKKRSVSSSIMDVSSERQMPDTKRIKLESLPKNFVPKQIKRTTSWGTIMSENVPTEQPTAISNPEEIPRTEEVSHTQVTYGSIQDKKRTASLEKPMRRQTRNQTKELDS

Target ORF information:

RefSeq Version	NM_001181523.1
Organism	Saccharomyces cerevisiae S288C
Definition	Saccharomyces cerevisiae S288C protein kinase activating protein DPB11 (DPB11), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001181523.1

ORF Insert Sequence:

ATGAAGCCCT TTCAAGGAAT AACATTTTGT CCGACTGCAA TAAATAATGA AATCTTAGCT 
AAAAAAATAT CAAAGAAAAT TATTAAGTTA GGAGGTATAT TTTCTAAAGA TTTGACCAGA 
CAAGTTAATG TCCTTGTTGT TGGGTCCACC ACCAATACAA ACAAATTTAA ATTTGCGGTG 
AAGCATAGAT TCGATATCAT ATTTATTGAC ATTCAAGCCA TTGATGACAT TTATCAGCTC 
TGGCTGTCTG GAGAAAATAT ATTGCCGGAC TCTAATACGG CAACAATGAC AGGATCCACC 
TATGAGATGC TGAAGATACT TTACCGGCGA TTTTCGTTTA AATATTTGCA TAACTTCAAT 
ATCTTCATTG GCAGAATAAC CGACACTAAC ATAACTTCCA TCGATTCATT GGTTAGGAGT 
ATTAAAAAGT TAGGGTGTTC TAGTTATAAC TATCAAAACT TTGTAATCAA AGATACCTCT 
TCACACAATG ATGACGATGA TCAAGGTCAG AATGGGCAGA TATCCATTTT TGTTACGGAT 
ACTTTACTAG GAGCAAGAGT TAACGCTGCT ATTGAACAAA ATATACCTAT TGTACATTTC 
AAATGGATTC TCGATTGCCA AAAAAGAAGC GCACTGTTAC CGTACGATCC CTATTATCTT 
TTACCAAATA TTAAAGACCT GCCCTATGAT TCAATCGGAT CAAACTCTTG CGATTGCTGG 
GATAAAATAA ACACCACCTT TCCAACCAAT ATCGATGCCC AATCCTCTCT GCAGCGGCAG 
CAATCCTCCA GTACCCTGAC TCCTTCGCTC CCGAAAACAT CATCCCTCTT GAATAAATTT 
AAACCAAAGG GTGAAAAAAT ATGGGACAAG GCAATGTCTC TACAGCAGCA TAGTAAGACA 
AATTTTTCAG TACTAGGGCA GTCACCATTA TCGATAAATA ACAAACAAGA AGATTTAAGT 
GATAATAGTA CTTTGATTTT CAAAAATTGT GCGTTTATCA TTCATCATAT TTTTCCTGGC 
AATCACCGTT CAATACTAAC CAAAATTGTT GTTCAAAATG GGGGTAAAAT TGAGACGTCT 
TACTTGAGCG GCATTTACGA TCATTCTTAT TACATCATTC CAAGTAATAA AGCTCTTGAC 
TCCTTCAATG ATTTACCTGA GATTATAGAT GATAATGATG GTATAGTTAC AGAATTTTTC 
ATTGAACGCT GCTTGTATTA TCAAAAATTA CTACACCCAA TAGATTTATG GTCAAAACCC 
TTCCTCAGCA CAATAGAGTT TCAAGTTTCG TCTTCTTCAA AGTTATTGCA TCATGAATTT 
TCTTCTTCCC CTTTTCTGAA TGTTACTATC ACTGGATTCT CTGGCGTAGA GCTGTTACAT 
CTGACTAAAG TATTAAATCT TCTAAAACCA ATGGGCATCA ATTATGTAGA ATACCTCAAT 
AAATCCACTG ACATTCTGCT AATCAACTTA GCAGCTTTAC CCAGTATCCC GAAAACCCAT 
CCGTTATGGT CGAATGAATT TAGCGATCTT TTTACTCAGT TTTGCATTAA TAACAATAAT 
GATGATCCTG GTGATAATAA CAGAAAAGAT TTTCAAAATA ATTCAATCTT GAGAAATTCG 
ATGAAAAGGA AAATTGAATA TATCAAGAAA TTCCACTCCA TACCGGTAGT TACTCCAGCA 
TTTATTTTTA AATTATTGTC CGCTGCATCT GGAGAAAATA ATGAAATCTT TTTAAACAAT 
ATCAAGTGGT GTATTATCTG CCCAAGAGGA CACAAGGACG ATTTTAAATG TAAGATAAAA 
AAACCATACT ATACCAGCAT TAGTTCAGAA AAAAAGTACC AAAACAATGA TCCAAAAATC 
GACAAAACTA TTCTTTTGAA AAGAAACAAT TCCTCATTAT CGGAGCACTC TATGAAAGAT 
ACCAAAAACG AATTATTGCA GAAAATTAGA GAAACTGATT CTGGAAGAAA AAAGCGTAGT 
GTCTCATCGA GTATCATGGA TGTTTCTTCA GAGAGACAAA TGCCGGATAC GAAAAGGATC 
AAGTTGGAGT CACTGCCAAA AAATTTCGTT CCTAAACAAA TTAAACGAAC CACGAGTTGG 
GGCACAATAA TGTCAGAAAA TGTGCCTACA GAGCAGCCGA CTGCAATTTC TAATCCAGAA 
GAGATCCCAA GAACTGAGGA AGTTTCACAT ACTCAAGTTA CCTATGGCTC CATTCAAGAT 
AAGAAACGTA CTGCCTCTTT AGAAAAACCT ATGAGACGAC AGACAAGAAA TCAGACAAAG 
GAATTAGATT CTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Life with 6000 genes.
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)
Goffeau A,Barrell BG,Bussey H,Davis RW,Dujon B,Feldmann H,Galibert F,Hoheisel JD,Jacq C,Johnston M,Louis EJ,Mewes HW,Murakami Y,Philippsen P,Tettelin H,Oliver SG

Complete nucleotide sequence of Saccharomyces cerevisiae chromosome X.
The EMBO journal15(9)2031-49(1996 May)
Galibert F,Alexandraki D,Baur A,Boles E,Chalwatzis N,Chuat JC,Coster F,Cziepluch C,De Haan M,Domdey H,Durand P,Entian KD,Gatius M,Goffeau A,Grivell LA,Hennemann A,Herbert CJ,Heumann K,Hilger F,Hollenberg CP,Huang ME,Jacq C,Jauniaux JC,Katsoulou C,Karpfinger-Hartl L

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DPB11 cDNA ORF clone, Saccharomyces cerevisiae S288C

Gene

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gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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