TIF4631 cDNA ORF clone, Saccharomyces cerevisiae S288C

Gene
Clones
gRNAs

Summary

Gene Symbol	TIF4631
Entrez Gene ID	853071
Full Name	translation initiation factor eIF4G
Gene Type	protein-coding
Organism	Saccharomyces cerevisiae S288C

mRNA and Protein(s)

mRNA	Protein	Name
NM_001181291.3	NP_011678.3	translation initiation factor eIF4G

Pathways from BioSystems

Homologies

Saccharomyces cerevisiae (baker's yeast)	TIF4631	NP_011678.3
Eremothecium gossypii	AGOS_AGL044C	NP_986622.2

Gene Ontology
Bibliography

Related articles in PubMed

The nucleotide sequence of Saccharomyces cerevisiae chromosome VII.
Tettelin H, Agostoni Carbone ML, Albermann K, Albers M, Arroyo J, Backes U, Barreiros T, Bertani I, Bjourson AJ, Br?ckner M, Bruschi CV, Carignani G, Castagnoli L, Cerdan E, Clemente ML, Coblenz A, Coglievina M, Coissac E, Defoor E, Del Bino S, Delius H, Delneri D, de Wergifosse P, Dujon B, Kleine K
Nature387(6632 Suppl)81-4(1997 May)

Life with 6000 genes.
Goffeau A, Barrell BG, Bussey H, Davis RW, Dujon B, Feldmann H, Galibert F, Hoheisel JD, Jacq C, Johnston M, Louis EJ, Mewes HW, Murakami Y, Philippsen P, Tettelin H, Oliver SG
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Pub1p C-terminal RRM domain interacts with Tif4631p through a conserved region neighbouring the Pab1p binding site
Title: Pub1p C-terminal RRM domain interacts with Tif4631p through a conserved region neighbouring the Pab1p binding site.

The RNA-binding region (RNA1) in the N-terminal domain (NTD) of yeast eIF4G1 can functionally substitute for the PABP-binding segment to rescue the function of an eIF4G1-459 mutant impaired for eIF4E binding.
Title: Multiple elements in the eIF4G1 N-terminus promote assembly of eIF4G1?PABP mRNPs in vivo.

Data show that that deletion of eIF4G1, but not eIF4G2, impairs growth and global translation initiation rates.
Title: Functional overlap between eIF4G isoforms in Saccharomyces cerevisiae.

involvement of yeast eIF4G in pre-mRNA metabolism; Tif4631p and Tif4632p interact efficiently with U1 snRNA; Tif4631p and Tif4632p associate with protein components of the splicing machinery, namely Snu71p and Prp11p.
Title: Interaction of yeast eIF4G with spliceosome components: implications in pre-mRNA processing events.

The following TIF4631 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the TIF4631 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OSi02384	NM_001181291.3 Latest version!	Saccharomyces cerevisiae S288C translation initiation factor eIF4G (TIF4631), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

TIF4631 ( NM_001181291.3 ) cDNA ORF clone, Saccharomyces cerevisiae S288C -> NP_011678.3 Saccharomyces cerevisiae S288C translation initiation factor eIF4G (TIF4631), partial mRNA.

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CloneID	OSi02384
Clone ID Related Accession (Same CDS sequence)	NM_001181291.3
Accession Version	NM_001181291.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2859bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-31
Organism	Saccharomyces cerevisiae S288C
Product	translation initiation factor eIF4G
Comment	Comment: REVIEWED REFSEQ: This record has been curated by SGD. This record is derived from an annotated genomic sequence (NC_001139). On Jul 30, 2012 this sequence version replaced NM_001181291.2. ##Genome-Annotation-Data-START## Annotation Provider :: SGD Annotation Status :: Full Annotation Annotation Version :: R64-2-1 URL :: http://www.yeastgenome.org/ ##Genome-Annotation-Data-END## COMPLETENESS: incomplete on both ends.

ATGACAGACG AAACTGCTCA CCCGACACAA TCTGCTTCAA AGCAGGAATC TGCTGCTCTG 
AAACAGACAG GCGATGACCA GCAGGAATCT CAGCAACAAC GTGGCTACAC CAACTACAAC 
AATGGTAGCA ACTATACCCA AAAGAAGCCT TACAACAGCA ACAGACCTCA TCAGCAAAGA 
GGTGGTAAAT TTGGACCAAA CAGATATAAC AACCGTGGCA ACTATAATGG TGGCGGTAGT 
TTCAGAGGTG GACACATGGG AGCCAACAGC TCAAACGTGC CATGGACTGG TTACTATAAT 
AACTACCCCG TTTACTACCA GCCCCAGCAA ATGGCGGCCG CTGGTAGTGC CCCTGCTAAT 
CCAATTCCTG TCGAAGAGAA GTCACCTGTT CCAACTAAGA TAGAAATCAC TACCAAGTCT 
GGTGAACACT TAGATTTGAA AGAACAGCAT AAAGCCAAGC TACAGTCTCA GGAAAGATCT 
ACTGTGTCTC CGCAACCAGA GTCAAAGTTA AAAGAAACTT CTGATTCTAC TTCTACTTCT 
ACTCCAACTC CTACCCCTTC CACTAATGAC TCTAAGGCCA GTTCTGAAGA AAATATATCT 
GAAGCTGAAA AGACAAGAAG AAATTTCATC GAGCAAGTTA AACTTCGTAA AGCTGCTTTA 
GAAAAGAAGA GAAAGGAGCA ACTTGAAGGT TCTAGTGGCA ACAATAATAT TCCAATGAAG 
ACTACCCCAG AAAATGTGGA AGAGAAGGGA TCGGACAAAC CTGAGGTAAC CGAAAAAACC 
AAGCCTGCTG AAGAAAAATC TGCTGAGCCA GAAGTTAAGC AAGAAACTCC AGCTGAAGAA 
GGTGAACAAG GAGAAAAGGG CCAAATAAAG GAAGAATCCA CTCCAAAGGT GTTAACCTTT 
GCTGAACGTT TGAAGTTGAA GAAACAACAA AAAGAACGAG AAGAAAAAAC GGAGGGGAAG 
GAAAACAAGG AAGTGCCAGT ACAGGAAGAG ACTAAGAGCG CCATCGAGTC TGCTCCTGTG 
CCTCCCAGTG AACAGGTTAA AGAAGAAACT GAGGTCGCCG AAACCGAACA GTCAAACATT 
GATGAATCTG CAACAACTCC GGCTATCCCT ACTAAATCTG ATGAAGCTGA AGCTGAAGTT 
GAGGCTGAAG CAGGTGATGC TGGAACAAAG ATTGGACTTG AAGCTGAAAT CGAAACTACA 
ACTGATGAAA CTGATGATGG TACAAACACC GTTTCTCACA TATTGAATGT ATTGAAAGAT 
GCTACACCAA TTGAAGATGT CTTTTCTTTT AACTATCCAG AAGGTATTGA AGGTCCTGAT 
ATCAAATACA AGAAAGAACA CGTCAAATAT ACATATGGCC CAACTTTCTT GCTTCAATTT 
AAGGACAAAC TAAACGTCAA AGCGGACGCC GAATGGGTCC AAAGCACTGC TTCTAAAATT 
GTCATTCCAC CAGGAATGGG CAGAGGAAAT AGATCTAGAG ATTCTGGCAG ATTCGGCAAT 
AATTCTAGTC GTGGCCATGA CTTTAGAAAT ACCTCAGTGA GAAATATGGA TGACAGAGCT 
AATTCAAGAA CTTCATCAAA GAGAAGATCA AAGAGAATGA ATGACGACAG GAGATCTAAT 
AGATCTTATA CATCAAGAAG AGACCGCGAG AGAGGCTCCT ACAGAAATGA AGAGAAAAGA 
GAGGACGATA AACCAAAGGA AGAAGTTGCT CCACTTGTTC CAAGTGCTAA TAGGTGGGTG 
CCAAAATTCA AGTCTAAAAA GACTGAAAAG AAGCTAGCCC CTGACGGAAA GACCGAACTA 
TTGGACAAGG ATGAAGTTGA AAGAAAGATG AAGTCACTAC TGAATAAACT TACTTTGGAA 
ATGTTCGATG CTATTTCATC TGAAATTTTG GCTATTGCAA ACATATCAGT ATGGGAAACA 
AATGGTGAAA CGTTAAAGGC TGTGATAGAA CAGATTTTCT TAAAGGCCTG TGACGAACCC 
CATTGGTCTT CTATGTACGC GCAATTATGT GGTAAAGTAG TTAAAGAATT AAACCCAGAT 
ATAACAGATG AAACTAATGA AGGTAAGACA GGTCCAAAAT TGGTTTTGCA TTACTTGGTT 
GCTAGATGTC ATGCCGAATT TGACAAGGGT TGGACCGATA AATTACCCAC AAACGAAGAT 
GGTACTCCAT TAGAACCTGA GATGATGTCA GAAGAGTACT ATGCAGCTGC TTCTGCTAAG 
AGAAGAGGTT TAGGTTTGGT TCGTTTCATC GGTTTCCTAT ACCGTCTGAA TCTGTTGACT 
GGAAAGATGA TGTTCGAATG TTTCCGTAGA CTAATGAAGG ATTTGACGGA TTCTCCATCT 
GAAGAAACTC TAGAGTCCGT TGTTGAATTG TTAAATACTG TTGGTGAACA ATTTGAGACC 
GATAGTTTCA GAACGGGTCA AGCAACATTG GAGGGTTCGC AATTGCTCGA TAGTTTGTTC 
GGTATTTTAG ACAATATTAT TCAAACTGCT AAGATTTCTA GTAGAATCAA GTTCAAGTTG 
ATCGACATTA AAGAATTAAG GCACGACAAG AACTGGAACA GTGATAAGAA GGACAACGGT 
CCTAAGACCA TTCAACAGAT TCATGAGGAA GAGGAGAGAC AACGTCAATT GAAGAATAAT 
TCAAGATCTA ATTCAAGACG TACGAACAAC TCCAGTAATA GACACTCTTT CAGAAGGGAC 
GCTCCTCCTG CATCAAAAGA TAGCTTTATC ACAACCAGAA CATATTCTCA AAGAAATAGC 
CAAAGGGCTC CTCCTCCAAA GGAAGAACCA GCTGCACCAA CTTCTACCGC AACAAATATG 
TTCAGTGCAT TAATGGGAGA AAGTGATGAC GAAGAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_011678.3
CDS	1..2859
Translation	MTDETAHPTQSASKQESAALKQTGDDQQESQQQRGYTNYNNGSNYTQKKPYNSNRPHQQRGGKFGPNRYNNRGNYNGGGSFRGGHMGANSSNVPWTGYYNNYPVYYQPQQMAAAGSAPANPIPVEEKSPVPTKIEITTKSGEHLDLKEQHKAKLQSQERSTVSPQPESKLKETSDSTSTSTPTPTPSTNDSKASSEENISEAEKTRRNFIEQVKLRKAALEKKRKEQLEGSSGNNNIPMKTTPENVEEKGSDKPEVTEKTKPAEEKSAEPEVKQETPAEEGEQGEKGQIKEESTPKVLTFAERLKLKKQQKEREEKTEGKENKEVPVQEETKSAIESAPVPPSEQVKEETEVAETEQSNIDESATTPAIPTKSDEAEAEVEAEAGDAGTKIGLEAEIETTTDETDDGTNTVSHILNVLKDATPIEDVFSFNYPEGIEGPDIKYKKEHVKYTYGPTFLLQFKDKLNVKADAEWVQSTASKIVIPPGMGRGNRSRDSGRFGNNSSRGHDFRNTSVRNMDDRANSRTSSKRRSKRMNDDRRSNRSYTSRRDRERGSYRNEEKREDDKPKEEVAPLVPSANRWVPKFKSKKTEKKLAPDGKTELLDKDEVERKMKSLLNKLTLEMFDAISSEILAIANISVWETNGETLKAVIEQIFLKACDEPHWSSMYAQLCGKVVKELNPDITDETNEGKTGPKLVLHYLVARCHAEFDKGWTDKLPTNEDGTPLEPEMMSEEYYAAASAKRRGLGLVRFIGFLYRLNLLTGKMMFECFRRLMKDLTDSPSEETLESVVELLNTVGEQFETDSFRTGQATLEGSQLLDSLFGILDNIIQTAKISSRIKFKLIDIKELRHDKNWNSDKKDNGPKTIQQIHEEEERQRQLKNNSRSNSRRTNNSSNRHSFRRDAPPASKDSFITTRTYSQRNSQRAPPPKEEPAAPTSTATNMFSALMGESDDEE

Target ORF information:

RefSeq Version	NM_001181291.3
Organism	Saccharomyces cerevisiae S288C
Definition	Saccharomyces cerevisiae S288C translation initiation factor eIF4G (TIF4631), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001181291.3

ORF Insert Sequence:

ATGACAGACG AAACTGCTCA CCCGACACAA TCTGCTTCAA AGCAGGAATC TGCTGCTCTG 
AAACAGACAG GCGATGACCA GCAGGAATCT CAGCAACAAC GTGGCTACAC CAACTACAAC 
AATGGTAGCA ACTATACCCA AAAGAAGCCT TACAACAGCA ACAGACCTCA TCAGCAAAGA 
GGTGGTAAAT TTGGACCAAA CAGATATAAC AACCGTGGCA ACTATAATGG TGGCGGTAGT 
TTCAGAGGTG GACACATGGG AGCCAACAGC TCAAACGTGC CATGGACTGG TTACTATAAT 
AACTACCCCG TTTACTACCA GCCCCAGCAA ATGGCGGCCG CTGGTAGTGC CCCTGCTAAT 
CCAATTCCTG TCGAAGAGAA GTCACCTGTT CCAACTAAGA TAGAAATCAC TACCAAGTCT 
GGTGAACACT TAGATTTGAA AGAACAGCAT AAAGCCAAGC TACAGTCTCA GGAAAGATCT 
ACTGTGTCTC CGCAACCAGA GTCAAAGTTA AAAGAAACTT CTGATTCTAC TTCTACTTCT 
ACTCCAACTC CTACCCCTTC CACTAATGAC TCTAAGGCCA GTTCTGAAGA AAATATATCT 
GAAGCTGAAA AGACAAGAAG AAATTTCATC GAGCAAGTTA AACTTCGTAA AGCTGCTTTA 
GAAAAGAAGA GAAAGGAGCA ACTTGAAGGT TCTAGTGGCA ACAATAATAT TCCAATGAAG 
ACTACCCCAG AAAATGTGGA AGAGAAGGGA TCGGACAAAC CTGAGGTAAC CGAAAAAACC 
AAGCCTGCTG AAGAAAAATC TGCTGAGCCA GAAGTTAAGC AAGAAACTCC AGCTGAAGAA 
GGTGAACAAG GAGAAAAGGG CCAAATAAAG GAAGAATCCA CTCCAAAGGT GTTAACCTTT 
GCTGAACGTT TGAAGTTGAA GAAACAACAA AAAGAACGAG AAGAAAAAAC GGAGGGGAAG 
GAAAACAAGG AAGTGCCAGT ACAGGAAGAG ACTAAGAGCG CCATCGAGTC TGCTCCTGTG 
CCTCCCAGTG AACAGGTTAA AGAAGAAACT GAGGTCGCCG AAACCGAACA GTCAAACATT 
GATGAATCTG CAACAACTCC GGCTATCCCT ACTAAATCTG ATGAAGCTGA AGCTGAAGTT 
GAGGCTGAAG CAGGTGATGC TGGAACAAAG ATTGGACTTG AAGCTGAAAT CGAAACTACA 
ACTGATGAAA CTGATGATGG TACAAACACC GTTTCTCACA TATTGAATGT ATTGAAAGAT 
GCTACACCAA TTGAAGATGT CTTTTCTTTT AACTATCCAG AAGGTATTGA AGGTCCTGAT 
ATCAAATACA AGAAAGAACA CGTCAAATAT ACATATGGCC CAACTTTCTT GCTTCAATTT 
AAGGACAAAC TAAACGTCAA AGCGGACGCC GAATGGGTCC AAAGCACTGC TTCTAAAATT 
GTCATTCCAC CAGGAATGGG CAGAGGAAAT AGATCTAGAG ATTCTGGCAG ATTCGGCAAT 
AATTCTAGTC GTGGCCATGA CTTTAGAAAT ACCTCAGTGA GAAATATGGA TGACAGAGCT 
AATTCAAGAA CTTCATCAAA GAGAAGATCA AAGAGAATGA ATGACGACAG GAGATCTAAT 
AGATCTTATA CATCAAGAAG AGACCGCGAG AGAGGCTCCT ACAGAAATGA AGAGAAAAGA 
GAGGACGATA AACCAAAGGA AGAAGTTGCT CCACTTGTTC CAAGTGCTAA TAGGTGGGTG 
CCAAAATTCA AGTCTAAAAA GACTGAAAAG AAGCTAGCCC CTGACGGAAA GACCGAACTA 
TTGGACAAGG ATGAAGTTGA AAGAAAGATG AAGTCACTAC TGAATAAACT TACTTTGGAA 
ATGTTCGATG CTATTTCATC TGAAATTTTG GCTATTGCAA ACATATCAGT ATGGGAAACA 
AATGGTGAAA CGTTAAAGGC TGTGATAGAA CAGATTTTCT TAAAGGCCTG TGACGAACCC 
CATTGGTCTT CTATGTACGC GCAATTATGT GGTAAAGTAG TTAAAGAATT AAACCCAGAT 
ATAACAGATG AAACTAATGA AGGTAAGACA GGTCCAAAAT TGGTTTTGCA TTACTTGGTT 
GCTAGATGTC ATGCCGAATT TGACAAGGGT TGGACCGATA AATTACCCAC AAACGAAGAT 
GGTACTCCAT TAGAACCTGA GATGATGTCA GAAGAGTACT ATGCAGCTGC TTCTGCTAAG 
AGAAGAGGTT TAGGTTTGGT TCGTTTCATC GGTTTCCTAT ACCGTCTGAA TCTGTTGACT 
GGAAAGATGA TGTTCGAATG TTTCCGTAGA CTAATGAAGG ATTTGACGGA TTCTCCATCT 
GAAGAAACTC TAGAGTCCGT TGTTGAATTG TTAAATACTG TTGGTGAACA ATTTGAGACC 
GATAGTTTCA GAACGGGTCA AGCAACATTG GAGGGTTCGC AATTGCTCGA TAGTTTGTTC 
GGTATTTTAG ACAATATTAT TCAAACTGCT AAGATTTCTA GTAGAATCAA GTTCAAGTTG 
ATCGACATTA AAGAATTAAG GCACGACAAG AACTGGAACA GTGATAAGAA GGACAACGGT 
CCTAAGACCA TTCAACAGAT TCATGAGGAA GAGGAGAGAC AACGTCAATT GAAGAATAAT 
TCAAGATCTA ATTCAAGACG TACGAACAAC TCCAGTAATA GACACTCTTT CAGAAGGGAC 
GCTCCTCCTG CATCAAAAGA TAGCTTTATC ACAACCAGAA CATATTCTCA AAGAAATAGC 
CAAAGGGCTC CTCCTCCAAA GGAAGAACCA GCTGCACCAA CTTCTACCGC AACAAATATG 
TTCAGTGCAT TAATGGGAGA AAGTGATGAC GAAGAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The nucleotide sequence of Saccharomyces cerevisiae chromosome VII.
Nature387(6632 Suppl)81-4(1997 May)
Tettelin H,Agostoni Carbone ML,Albermann K,Albers M,Arroyo J,Backes U,Barreiros T,Bertani I,Bjourson AJ,Br?ckner M,Bruschi CV,Carignani G,Castagnoli L,Cerdan E,Clemente ML,Coblenz A,Coglievina M,Coissac E,Defoor E,Del Bino S,Delius H,Delneri D,de Wergifosse P,Dujon B,Kleine K

Life with 6000 genes.
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)
Goffeau A,Barrell BG,Bussey H,Davis RW,Dujon B,Feldmann H,Galibert F,Hoheisel JD,Jacq C,Johnston M,Louis EJ,Mewes HW,Murakami Y,Philippsen P,Tettelin H,Oliver SG

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TIF4631 cDNA ORF clone, Saccharomyces cerevisiae S288C

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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