SPT6 cDNA ORF clone, Saccharomyces cerevisiae S288C

Gene
Clones
gRNAs

Summary

Gene Symbol	SPT6
Entrez Gene ID	853011
Full Name	chromatin-remodeling histone chaperone SPT6
Synonyms	CRE2,SSN20
Gene Type	protein-coding
Organism	Saccharomyces cerevisiae S288C

mRNA and Protein(s)

mRNA	Protein	Name
NM_001181245.1	NP_011631.1	chromatin-remodeling histone chaperone SPT6

Pathways from BioSystems

Homologies

	SUPT6H	XP_001106850.2
Canis lupus familiaris (dog)	SUPT6H	XP_537747.2
Caenorhabditis elegans (roundworm)	emb-5	NP_497969.1
Magnaporthe oryzae (rice blast fungus)	MGG_11491	XP_003718184.1
Arabidopsis thaliana (thale cress)	AT1G63210	NP_176510.1
Gallus gallus (chicken)	SUPT6H	XP_423183.3
Schizosaccharomyces pombe (fission yeast)	spt6	NP_594487.2
Drosophila melanogaster (fruit fly)	Spt6	NP_651962.2
Kluyveromyces lactis	KLLA0B11385g	XP_452036.1
Eremothecium gossypii	AGOS_AAR031W	NP_982572.2
Neurospora crassa	NCU04611	XP_958854.1
Arabidopsis thaliana (thale cress)	GTB1	NP_001077775.1
Xenopus tropicalis (tropical clawed frog)	supt6h	NP_001072665.1
Homo sapiens (human)	SUPT6H	NP_003161.2
Pan troglodytes (chimpanzee)	SUPT6H	XP_001143115.2
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP003619	XP_313379.5
Saccharomyces cerevisiae (baker's yeast)	SPT6	NP_011631.1
Bos taurus (cattle)	SUPT6H	NP_001180055.1
Mus musculus (house mouse)	Supt6	NP_033323.2
Rattus norvegicus (Norway rat)	Supt6h	NP_001178749.1
Danio rerio (zebrafish)	supt6h	NP_660094.1

Gene Ontology
Bibliography

Related articles in PubMed

The nucleotide sequence of Saccharomyces cerevisiae chromosome VII.
Tettelin H, Agostoni Carbone ML, Albermann K, Albers M, Arroyo J, Backes U, Barreiros T, Bertani I, Bjourson AJ, Br?ckner M, Bruschi CV, Carignani G, Castagnoli L, Cerdan E, Clemente ML, Coblenz A, Coglievina M, Coissac E, Defoor E, Del Bino S, Delius H, Delneri D, de Wergifosse P, Dujon B, Kleine K
Nature387(6632 Suppl)81-4(1997 May)

Life with 6000 genes.
Goffeau A, Barrell BG, Bussey H, Davis RW, Dujon B, Feldmann H, Galibert F, Hoheisel JD, Jacq C, Johnston M, Louis EJ, Mewes HW, Murakami Y, Philippsen P, Tettelin H, Oliver SG
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Interactions between SPT6 and RNA Polymerase II
Title: Relationships Between RNA Polymerase II Activity and Spt Elongation Factors to Spt- Phenotype and Growth in Saccharomyces cerevisiae.

Results demonstrate that a fine-tuning of the Spt16 component of FACT by UPS is essential for proper chromatin reassembly and efficient transcriptional elongation, thus revealing a novel ubiquitin proteasome system regulation of FACT with physiological relevance in gene expression.
Title: Fine-Tuning of FACT by the Ubiquitin Proteasome System in Regulation of Transcriptional Elongation.

Histone chaperones FACT and Spt6 are important for appropriate H2AZ histone localization and for the functioning of RNA polymerase II.
Title: Histone chaperones FACT and Spt6 prevent histone variants from turning into histone deviants.

Spt6 and FACT have both overlapping and distinct functional interactions with histones and nucleosomes and that binding to each of the components of a nucleosome is not sufficient to produce nucleosome reorganization
Title: The Abundant Histone Chaperones Spt6 and FACT Collaborate to Assemble, Inspect, and Maintain Chromatin Structure in Saccharomyces cerevisiae.

Data show that the histone chaperones POB3 protein FACT and chromatin-remodeling histone chaperone Spt6 prevent widespread accumulation of histone H2A.Z.
Title: The Histone Chaperones FACT and Spt6 Restrict H2A.Z from Intragenic Locations.

The following SPT6 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the SPT6 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OSi02341	NM_001181245.1 Latest version!	Saccharomyces cerevisiae S288C chromatin-remodeling histone chaperone SPT6 (SPT6), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

SPT6 ( NM_001181245.1 ) cDNA ORF clone, Saccharomyces cerevisiae S288C -> NP_011631.1 Saccharomyces cerevisiae S288C chromatin-remodeling histone chaperone SPT6 (SPT6), partial mRNA.

Price & Availability↑

CloneID	OSi02341
Clone ID Related Accession (Same CDS sequence)	NM_001181245.1
Accession Version	NM_001181245.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4356bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-31
Organism	Saccharomyces cerevisiae S288C
Product	chromatin-remodeling histone chaperone SPT6
Comment	Comment: REVIEWED REFSEQ: This record has been curated by SGD. This record is derived from an annotated genomic sequence (NC_001139). ##Genome-Annotation-Data-START## Annotation Provider :: SGD Annotation Status :: Full Annotation Annotation Version :: R64-2-1 URL :: http://www.yeastgenome.org/ ##Genome-Annotation-Data-END## COMPLETENESS: incomplete on both ends.

ATGGAAGAGA CGGGAGATTC GAAGCTGGTC CCTAGGGACG AGGAAGAAAT AGTAAATGAC 
AACGATGAAA CTAAAGCGCC TAGTGAGGAA GAAGAAGGAG AAGATGTCTT TGACTCCTCT 
GAGGAAGACG AAGATATTGA TGAAGACGAA GATGAGGCAA GAAAAGTGCA AGAAGGCTTC 
ATTGTCAACG ATGATGATGA AAATGAAGAC CCAGGAACAA GTATTTCCAA AAAAAGAAGA 
AAACATAAAA GAAGAGAAAG AGAAGAAGAT GATCGACTAT CCGAAGATGA TTTGGATTTG 
TTAATGGAGA ATGCTGGTGT TGAACGTACC AAAGCGAGCT CCTCTTCAGG AAAATTTAAA 
AGATTAAAGA GAGTAGGAGA TGAAGGAAAC GCTGCAGAAT CAGAAAGTGA CAACGTCGCT 
GCATCAAGGC AAGATTCTAC GTCTAAATTG GAAGATTTCT TTTCTGAAGA TGAAGAAGAA 
GAAGAATCTG GTTTACGCAA CGGTAGGAAC AATGAATATG GGCGTGATGA AGAAGACCAT 
GAAAATAGGA ACAGAACAGC TGACAAAGGT GGAATCTTGG ACGAATTGGA TGATTTCATT 
GAAGATGATG AATTTTCTGA TGAGGACGAC GAAACCAGAC AAAGGAGGAT CCAAGAAAAG 
AAGCTTTTAA GAGAACAATC CATCAAACAA CCTACACAGA TTACTGGTCT ATCGTCGGAT 
AAGATTGACG AGATGTATGA CATTTTTGGT GATGGTCATG ACTACGATTG GGCTTTAGAA 
ATTGAAAATG AAGAACTAGA AAATGGTAAC GACAACAATG AAGCTGAAGA AGAAGAGATC 
GATGAAGAAA CTGGTGCTAT AAAAAGCACC AAGAAAAAGA TATCTCTACA AGACATTTAT 
GATTTAGAGG ATTTGAAAAA AAACTTGATG ACTGAAGGAG ACATGAAAAT TAGAAAGACA 
GATATTCCAG AAAGATATCA AGAATTAAGA GCAGGTATTA CTGACTACGG AAATATGTCA 
TCGGAGGATC AAGAATTAGA AAGAAACTGG ATAGCAGAAA AAATTTCTGT GGATAAGAAC 
TTCGATGCCA ATTATGATCT CACTGAATTT AAAGAAGCAA TTGGGAACGC AATCAAATTT 
ATCACCAAAG AAAACTTGGA AGTCCCTTTT ATATATGCTT ACCGTCGTAA CTATATTTCC 
TCAAGAGAAA AAGATGGGTT TCTTTTGACT GAAGATGACC TTTGGGATAT AGTTAGCCTT 
GACATCGAAT TTCACAGTCT TGTGAACAAA AAGGATTATG TCCAGAGATT TTATGCAGAA 
TTACATATCG ATGATCCTAT TGTCACTGAA TACTTCAAAA ATCAGAATAC TGCATCTATT 
GCGGAACTGA ATTCTCTACA GGATATTTAT GACTACCTAG AATTCAAATA TGCCAACGAA 
ATCAATGAAA TGTTTATAAA CCACACTGGA AAGACTGGTA AGAAACATTT GAAAAATTCC 
AGTTATGAAA AATTTAAAGC TAGTCCTCTT TATCAAGCGG TTAGTGATAT TGGTATATCA 
GCTGAGGATG TTGGTGAAAA TATCAGTTCC CAGCATCAAA TCCACCCTCC CGTAGATCAT 
CCAAGTTCCA AACCAGTAGA AGTGATAGAA TCTATATTGA ATGCAAACAG CGGTGATTTG 
CAAGTCTTTA CGTCCAATAC TAAGCTGGCA ATTGATACGG TCCAAAAATA CTACTCTTTG 
GAATTGTCTA AAAATACAAA AATAAGGGAA AAAGTTAGAT CCGATTTTTC CAAATATTAT 
CTGGCTGACG TTGTGTTAAC TGCTAAAGGT AAAAAAGAAA TTCAAAAGGG ATCTCTGTAT 
GAGGACATAA AATATGCCAT CAATAGAACT CCAATGCACT TCCGTAGGGA TCCAGACGTT 
TTTTTGAAAA TGGTCGAGGC TGAGTCTTTG AACCTGCTCA GTGTTAAGTT ACACATGTCG 
TCACAAGCCC AATATATAGA GCATTTATTC CAAATTGCAC TTGAAACTAC CAATACCTCG 
GACATCGCAA TAGAATGGAA TAACTTCCGT AAACTGGCAT TCAACCAAGC GATGGACAAG 
ATTTTCCAGG ATATATCTCA AGAAGTCAAA GACAATTTAA CAAAAAATTG TCAAAAATTG 
GTAGCCAAGA CTGTTCGCCA TAAGTTTATG ACAAAATTAG ACCAGGCTCC ATTCATTCCT 
AATGTCAGGG ATCCAAAAAT TCCAAAAATC TTATCTTTAA CCTGTGGACA GGGTAGATTC 
GGAGCCGACG CTATAATTGC TGTCTACGTC AACAGAAAGG GTGATTTTAT AAGAGATTAC 
AAGATTGTCG ACAATCCATT TGATAAGACG AATCCTGAAA AATTTGAAGA CACCTTGGAT 
AATATCATTC AAAGCTGTCA ACCGAATGCC ATCGGAATCA ATGGCCCTAA CCCAAAGACT 
CAAAAATTTT ACAAAAGATT ACAAGAAGTT CTACATAAGA AGCAAATCGT CGACAGTAGA 
GGACATACTA TTCCAATCAT TTACGTTGAG GACGAAGTCG CTATCCGTTA TCAGAATTCC 
GAAAGAGCTG CTCAAGAATT CCCTAATAAA CCTCCTCTAG TTAAATACTG TATCGCCTTG 
GCGCGCTATA TGCATTCCCC ATTGTTGGAA TATGCTAATT TAACAAGTGA AGAAGTGAGA 
TCATTGTCAA TTCATCCACA CCAAAATCTG TTATCCTCAG AACAATTGAG TTGGGCTCTT 
GAAACCGCTT TCGTTGATAT TGTCAACCTG GTAAGTGTTG AAGTTAACAA AGCCACAGAT 
AATAATTACT ACGCTAGTGC GCTGAAATAC ATCTCTGGCT TTGGAAAACG TAAAGCTATT 
GATTTCTTAC AGTCCCTTCA AAGGCTAAAT GAACCATTAC TGGCTCGTCA ACAATTAATT 
ACTCATAACA TTCTTCACAA GACTATTTTT ATGAATTCCG CGGGATTCCT CTATATCTCA 
TGGAATGAAA AAAGACAAAA ATACGAAGAT TTGGAACATG ATCAACTAGA TAGCACTAGA 
ATTCATCCAG AAGACTACCA TTTGGCCACC AAGGTTGCCG CTGATGCTTT AGAATACGAT 
CCTGATACTA TTGCCGAAAA AGAAGAACAG GGGACTATGA GTGAATTCAT TGAACTGTTG 
AGAGAAGATC CTGACCGTAG AGCTAAACTA GAATCACTAA ATCTAGAATC ATACGCAGAA 
GAACTTGAGA AGAATACCGG ATTAAGAAAA CTTAATAATC TAAATACAAT TGTCCTTGAA 
TTGTTGGATG GATTTGAAGA ATTGAGAAAT GACTTTCATC CTTTGCAAGG TGATGAAATT 
TTCCAAAGTT TGACTGGTGA GTCTGAAAAG ACGTTTTTCA AGGGTAGTAT TATTCCAGTC 
AGAGTAGAAA GATTCTGGCA CAACGATATA ATTTGCACTA CAAACTCTGA AGTTGAATGT 
GTAGTAAATG CTCAACGTCA CGCAGGTGCA CAATTAAGAA GACCTGCAAA TGAAATATAC 
GAAATTGGTA AAACATATCC AGCAAAGGTG ATATATATTG ACTATGCTAA TATTACTGCA 
GAAGTTTCCT TATTAGATCA TGATGTCAAA CAGCAATATG TTCCAATAAG CTACAGTAAA 
GATCCTTCCA TTTGGGACTT GAAACAAGAA CTGGAAGATG CCGAAGAGGA GAGGAAATTG 
ATGATGGCAG AAGCCCGTGC AAAGAGAACA CATCGTGTTA TCAATCATCC TTACTATTTC 
CCTTTCAACG GCAGACAGGC TGAGGATTAC TTAAGGAGTA AAGAACGTGG TGAATTCGTG 
ATCAGACAGT CTAGCCGAGG TGATGACCAC TTGGTTATCA CCTGGAAATT GGATAAGGAT 
TTGTTTCAAC ATATTGATAT CCAAGAATTA GAAAAAGAAA ATCCTTTGGC TTTAGGTAAA 
GTCTTGATTG TCGACAATCA GAAATACAAT GATTTAGACC AGATCATTGT AGAATATCTT 
CAAAACAAGG TAAGGCTCTT GAATGAAATG ACATCTAGTG AAAAATTCAA AAGCGGTACT 
AAGAAAGATG TGGTCAAGTT TATTGAAGAC TACTCTAGAG TGAATCCAAA TAAGTCTGTG 
TACTATTTCA GTTTGAACCA CGATAACCCT GGTTGGTTTT ACTTGATGTT CAAGATTAAC 
GCAAATAGCA AATTATACAC ATGGAATGTG AAATTAACGA ACACTGGTTA TTTCCTGGTA 
AACTACAATT ATCCAAGTGT TATCCAGCTT TGTAATGGTT TTAAGACGCT TCTAAAATCT 
AACAGTAGTA AGAATAGAAT GAACAACTAC CGTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_011631.1
CDS	1..4356
Translation	MEETGDSKLVPRDEEEIVNDNDETKAPSEEEEGEDVFDSSEEDEDIDEDEDEARKVQEGFIVNDDDENEDPGTSISKKRRKHKRREREEDDRLSEDDLDLLMENAGVERTKASSSSGKFKRLKRVGDEGNAAESESDNVAASRQDSTSKLEDFFSEDEEEEESGLRNGRNNEYGRDEEDHENRNRTADKGGILDELDDFIEDDEFSDEDDETRQRRIQEKKLLREQSIKQPTQITGLSSDKIDEMYDIFGDGHDYDWALEIENEELENGNDNNEAEEEEIDEETGAIKSTKKKISLQDIYDLEDLKKNLMTEGDMKIRKTDIPERYQELRAGITDYGNMSSEDQELERNWIAEKISVDKNFDANYDLTEFKEAIGNAIKFITKENLEVPFIYAYRRNYISSREKDGFLLTEDDLWDIVSLDIEFHSLVNKKDYVQRFYAELHIDDPIVTEYFKNQNTASIAELNSLQDIYDYLEFKYANEINEMFINHTGKTGKKHLKNSSYEKFKASPLYQAVSDIGISAEDVGENISSQHQIHPPVDHPSSKPVEVIESILNANSGDLQVFTSNTKLAIDTVQKYYSLELSKNTKIREKVRSDFSKYYLADVVLTAKGKKEIQKGSLYEDIKYAINRTPMHFRRDPDVFLKMVEAESLNLLSVKLHMSSQAQYIEHLFQIALETTNTSDIAIEWNNFRKLAFNQAMDKIFQDISQEVKDNLTKNCQKLVAKTVRHKFMTKLDQAPFIPNVRDPKIPKILSLTCGQGRFGADAIIAVYVNRKGDFIRDYKIVDNPFDKTNPEKFEDTLDNIIQSCQPNAIGINGPNPKTQKFYKRLQEVLHKKQIVDSRGHTIPIIYVEDEVAIRYQNSERAAQEFPNKPPLVKYCIALARYMHSPLLEYANLTSEEVRSLSIHPHQNLLSSEQLSWALETAFVDIVNLVSVEVNKATDNNYYASALKYISGFGKRKAIDFLQSLQRLNEPLLARQQLITHNILHKTIFMNSAGFLYISWNEKRQKYEDLEHDQLDSTRIHPEDYHLATKVAADALEYDPDTIAEKEEQGTMSEFIELLREDPDRRAKLESLNLESYAEELEKNTGLRKLNNLNTIVLELLDGFEELRNDFHPLQGDEIFQSLTGESEKTFFKGSIIPVRVERFWHNDIICTTNSEVECVVNAQRHAGAQLRRPANEIYEIGKTYPAKVIYIDYANITAEVSLLDHDVKQQYVPISYSKDPSIWDLKQELEDAEEERKLMMAEARAKRTHRVINHPYYFPFNGRQAEDYLRSKERGEFVIRQSSRGDDHLVITWKLDKDLFQHIDIQELEKENPLALGKVLIVDNQKYNDLDQIIVEYLQNKVRLLNEMTSSEKFKSGTKKDVVKFIEDYSRVNPNKSVYYFSLNHDNPGWFYLMFKINANSKLYTWNVKLTNTGYFLVNYNYPSVIQLCNGFKTLLKSNSSKNRMNNYR

Target ORF information:

RefSeq Version	NM_001181245.1
Organism	Saccharomyces cerevisiae S288C
Definition	Saccharomyces cerevisiae S288C chromatin-remodeling histone chaperone SPT6 (SPT6), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001181245.1

ORF Insert Sequence:

ATGGAAGAGA CGGGAGATTC GAAGCTGGTC CCTAGGGACG AGGAAGAAAT AGTAAATGAC 
AACGATGAAA CTAAAGCGCC TAGTGAGGAA GAAGAAGGAG AAGATGTCTT TGACTCCTCT 
GAGGAAGACG AAGATATTGA TGAAGACGAA GATGAGGCAA GAAAAGTGCA AGAAGGCTTC 
ATTGTCAACG ATGATGATGA AAATGAAGAC CCAGGAACAA GTATTTCCAA AAAAAGAAGA 
AAACATAAAA GAAGAGAAAG AGAAGAAGAT GATCGACTAT CCGAAGATGA TTTGGATTTG 
TTAATGGAGA ATGCTGGTGT TGAACGTACC AAAGCGAGCT CCTCTTCAGG AAAATTTAAA 
AGATTAAAGA GAGTAGGAGA TGAAGGAAAC GCTGCAGAAT CAGAAAGTGA CAACGTCGCT 
GCATCAAGGC AAGATTCTAC GTCTAAATTG GAAGATTTCT TTTCTGAAGA TGAAGAAGAA 
GAAGAATCTG GTTTACGCAA CGGTAGGAAC AATGAATATG GGCGTGATGA AGAAGACCAT 
GAAAATAGGA ACAGAACAGC TGACAAAGGT GGAATCTTGG ACGAATTGGA TGATTTCATT 
GAAGATGATG AATTTTCTGA TGAGGACGAC GAAACCAGAC AAAGGAGGAT CCAAGAAAAG 
AAGCTTTTAA GAGAACAATC CATCAAACAA CCTACACAGA TTACTGGTCT ATCGTCGGAT 
AAGATTGACG AGATGTATGA CATTTTTGGT GATGGTCATG ACTACGATTG GGCTTTAGAA 
ATTGAAAATG AAGAACTAGA AAATGGTAAC GACAACAATG AAGCTGAAGA AGAAGAGATC 
GATGAAGAAA CTGGTGCTAT AAAAAGCACC AAGAAAAAGA TATCTCTACA AGACATTTAT 
GATTTAGAGG ATTTGAAAAA AAACTTGATG ACTGAAGGAG ACATGAAAAT TAGAAAGACA 
GATATTCCAG AAAGATATCA AGAATTAAGA GCAGGTATTA CTGACTACGG AAATATGTCA 
TCGGAGGATC AAGAATTAGA AAGAAACTGG ATAGCAGAAA AAATTTCTGT GGATAAGAAC 
TTCGATGCCA ATTATGATCT CACTGAATTT AAAGAAGCAA TTGGGAACGC AATCAAATTT 
ATCACCAAAG AAAACTTGGA AGTCCCTTTT ATATATGCTT ACCGTCGTAA CTATATTTCC 
TCAAGAGAAA AAGATGGGTT TCTTTTGACT GAAGATGACC TTTGGGATAT AGTTAGCCTT 
GACATCGAAT TTCACAGTCT TGTGAACAAA AAGGATTATG TCCAGAGATT TTATGCAGAA 
TTACATATCG ATGATCCTAT TGTCACTGAA TACTTCAAAA ATCAGAATAC TGCATCTATT 
GCGGAACTGA ATTCTCTACA GGATATTTAT GACTACCTAG AATTCAAATA TGCCAACGAA 
ATCAATGAAA TGTTTATAAA CCACACTGGA AAGACTGGTA AGAAACATTT GAAAAATTCC 
AGTTATGAAA AATTTAAAGC TAGTCCTCTT TATCAAGCGG TTAGTGATAT TGGTATATCA 
GCTGAGGATG TTGGTGAAAA TATCAGTTCC CAGCATCAAA TCCACCCTCC CGTAGATCAT 
CCAAGTTCCA AACCAGTAGA AGTGATAGAA TCTATATTGA ATGCAAACAG CGGTGATTTG 
CAAGTCTTTA CGTCCAATAC TAAGCTGGCA ATTGATACGG TCCAAAAATA CTACTCTTTG 
GAATTGTCTA AAAATACAAA AATAAGGGAA AAAGTTAGAT CCGATTTTTC CAAATATTAT 
CTGGCTGACG TTGTGTTAAC TGCTAAAGGT AAAAAAGAAA TTCAAAAGGG ATCTCTGTAT 
GAGGACATAA AATATGCCAT CAATAGAACT CCAATGCACT TCCGTAGGGA TCCAGACGTT 
TTTTTGAAAA TGGTCGAGGC TGAGTCTTTG AACCTGCTCA GTGTTAAGTT ACACATGTCG 
TCACAAGCCC AATATATAGA GCATTTATTC CAAATTGCAC TTGAAACTAC CAATACCTCG 
GACATCGCAA TAGAATGGAA TAACTTCCGT AAACTGGCAT TCAACCAAGC GATGGACAAG 
ATTTTCCAGG ATATATCTCA AGAAGTCAAA GACAATTTAA CAAAAAATTG TCAAAAATTG 
GTAGCCAAGA CTGTTCGCCA TAAGTTTATG ACAAAATTAG ACCAGGCTCC ATTCATTCCT 
AATGTCAGGG ATCCAAAAAT TCCAAAAATC TTATCTTTAA CCTGTGGACA GGGTAGATTC 
GGAGCCGACG CTATAATTGC TGTCTACGTC AACAGAAAGG GTGATTTTAT AAGAGATTAC 
AAGATTGTCG ACAATCCATT TGATAAGACG AATCCTGAAA AATTTGAAGA CACCTTGGAT 
AATATCATTC AAAGCTGTCA ACCGAATGCC ATCGGAATCA ATGGCCCTAA CCCAAAGACT 
CAAAAATTTT ACAAAAGATT ACAAGAAGTT CTACATAAGA AGCAAATCGT CGACAGTAGA 
GGACATACTA TTCCAATCAT TTACGTTGAG GACGAAGTCG CTATCCGTTA TCAGAATTCC 
GAAAGAGCTG CTCAAGAATT CCCTAATAAA CCTCCTCTAG TTAAATACTG TATCGCCTTG 
GCGCGCTATA TGCATTCCCC ATTGTTGGAA TATGCTAATT TAACAAGTGA AGAAGTGAGA 
TCATTGTCAA TTCATCCACA CCAAAATCTG TTATCCTCAG AACAATTGAG TTGGGCTCTT 
GAAACCGCTT TCGTTGATAT TGTCAACCTG GTAAGTGTTG AAGTTAACAA AGCCACAGAT 
AATAATTACT ACGCTAGTGC GCTGAAATAC ATCTCTGGCT TTGGAAAACG TAAAGCTATT 
GATTTCTTAC AGTCCCTTCA AAGGCTAAAT GAACCATTAC TGGCTCGTCA ACAATTAATT 
ACTCATAACA TTCTTCACAA GACTATTTTT ATGAATTCCG CGGGATTCCT CTATATCTCA 
TGGAATGAAA AAAGACAAAA ATACGAAGAT TTGGAACATG ATCAACTAGA TAGCACTAGA 
ATTCATCCAG AAGACTACCA TTTGGCCACC AAGGTTGCCG CTGATGCTTT AGAATACGAT 
CCTGATACTA TTGCCGAAAA AGAAGAACAG GGGACTATGA GTGAATTCAT TGAACTGTTG 
AGAGAAGATC CTGACCGTAG AGCTAAACTA GAATCACTAA ATCTAGAATC ATACGCAGAA 
GAACTTGAGA AGAATACCGG ATTAAGAAAA CTTAATAATC TAAATACAAT TGTCCTTGAA 
TTGTTGGATG GATTTGAAGA ATTGAGAAAT GACTTTCATC CTTTGCAAGG TGATGAAATT 
TTCCAAAGTT TGACTGGTGA GTCTGAAAAG ACGTTTTTCA AGGGTAGTAT TATTCCAGTC 
AGAGTAGAAA GATTCTGGCA CAACGATATA ATTTGCACTA CAAACTCTGA AGTTGAATGT 
GTAGTAAATG CTCAACGTCA CGCAGGTGCA CAATTAAGAA GACCTGCAAA TGAAATATAC 
GAAATTGGTA AAACATATCC AGCAAAGGTG ATATATATTG ACTATGCTAA TATTACTGCA 
GAAGTTTCCT TATTAGATCA TGATGTCAAA CAGCAATATG TTCCAATAAG CTACAGTAAA 
GATCCTTCCA TTTGGGACTT GAAACAAGAA CTGGAAGATG CCGAAGAGGA GAGGAAATTG 
ATGATGGCAG AAGCCCGTGC AAAGAGAACA CATCGTGTTA TCAATCATCC TTACTATTTC 
CCTTTCAACG GCAGACAGGC TGAGGATTAC TTAAGGAGTA AAGAACGTGG TGAATTCGTG 
ATCAGACAGT CTAGCCGAGG TGATGACCAC TTGGTTATCA CCTGGAAATT GGATAAGGAT 
TTGTTTCAAC ATATTGATAT CCAAGAATTA GAAAAAGAAA ATCCTTTGGC TTTAGGTAAA 
GTCTTGATTG TCGACAATCA GAAATACAAT GATTTAGACC AGATCATTGT AGAATATCTT 
CAAAACAAGG TAAGGCTCTT GAATGAAATG ACATCTAGTG AAAAATTCAA AAGCGGTACT 
AAGAAAGATG TGGTCAAGTT TATTGAAGAC TACTCTAGAG TGAATCCAAA TAAGTCTGTG 
TACTATTTCA GTTTGAACCA CGATAACCCT GGTTGGTTTT ACTTGATGTT CAAGATTAAC 
GCAAATAGCA AATTATACAC ATGGAATGTG AAATTAACGA ACACTGGTTA TTTCCTGGTA 
AACTACAATT ATCCAAGTGT TATCCAGCTT TGTAATGGTT TTAAGACGCT TCTAAAATCT 
AACAGTAGTA AGAATAGAAT GAACAACTAC CGTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The nucleotide sequence of Saccharomyces cerevisiae chromosome VII.
Nature387(6632 Suppl)81-4(1997 May)
Tettelin H,Agostoni Carbone ML,Albermann K,Albers M,Arroyo J,Backes U,Barreiros T,Bertani I,Bjourson AJ,Br?ckner M,Bruschi CV,Carignani G,Castagnoli L,Cerdan E,Clemente ML,Coblenz A,Coglievina M,Coissac E,Defoor E,Del Bino S,Delius H,Delneri D,de Wergifosse P,Dujon B,Kleine K

Life with 6000 genes.
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)
Goffeau A,Barrell BG,Bussey H,Davis RW,Dujon B,Feldmann H,Galibert F,Hoheisel JD,Jacq C,Johnston M,Louis EJ,Mewes HW,Murakami Y,Philippsen P,Tettelin H,Oliver SG

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SPT6 cDNA ORF clone, Saccharomyces cerevisiae S288C

Gene

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gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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