TFC4 cDNA ORF clone, Saccharomyces cerevisiae S288C

Gene
Clones
gRNAs

Summary

Gene Symbol	TFC4
Entrez Gene ID	852938
Full Name	transcription factor TFIIIC subunit TFC4
Synonyms	PCF1
Gene Type	protein-coding
Organism	Saccharomyces cerevisiae S288C

mRNA and Protein(s)

mRNA	Protein	Name
NM_001181176.3	NP_011561.3	transcription factor TFIIIC subunit TFC4

Pathways from BioSystems

Homologies

Saccharomyces cerevisiae (baker's yeast)	TFC4	NP_011561.3
Kluyveromyces lactis	KLLA0C02453g	XP_452306.1
Schizosaccharomyces pombe (fission yeast)	sfc4	NP_587924.1
Eremothecium gossypii	AGOS_AFR663W	NP_986211.2

Gene Ontology
Bibliography

Related articles in PubMed

The nucleotide sequence of Saccharomyces cerevisiae chromosome VII.
Tettelin H, Agostoni Carbone ML, Albermann K, Albers M, Arroyo J, Backes U, Barreiros T, Bertani I, Bjourson AJ, Br?ckner M, Bruschi CV, Carignani G, Castagnoli L, Cerdan E, Clemente ML, Coblenz A, Coglievina M, Coissac E, Defoor E, Del Bino S, Delius H, Delneri D, de Wergifosse P, Dujon B, Kleine K
Nature387(6632 Suppl)81-4(1997 May)

Life with 6000 genes.
Goffeau A, Barrell BG, Bussey H, Davis RW, Dujon B, Feldmann H, Galibert F, Hoheisel JD, Jacq C, Johnston M, Louis EJ, Mewes HW, Murakami Y, Philippsen P, Tettelin H, Oliver SG
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The data are consistent with this peptide causing a conformational change in TFIIIC that overcomes Tfc4 autoinhibition of Brf1 binding and suggest a structural model for the Brf1-Tfc4 interaction.
Title: Interactions of Brf1 peptides with the tetratricopeptide repeat-containing subunit of TFIIIC inhibit and promote preinitiation complex assembly.

The following TFC4 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the TFC4 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OSi02278	NM_001181176.3 Latest version!	Saccharomyces cerevisiae S288C transcription factor TFIIIC subunit TFC4 (TFC4), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

TFC4 ( NM_001181176.3 ) cDNA ORF clone, Saccharomyces cerevisiae S288C -> NP_011561.3 Saccharomyces cerevisiae S288C transcription factor TFIIIC subunit TFC4 (TFC4), partial mRNA.

Price & Availability↑

CloneID	OSi02278
Clone ID Related Accession (Same CDS sequence)	NM_001181176.3
Accession Version	NM_001181176.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3078bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-31
Organism	Saccharomyces cerevisiae S288C
Product	transcription factor TFIIIC subunit TFC4
Comment	Comment: REVIEWED REFSEQ: This record has been curated by SGD. This record is derived from an annotated genomic sequence (NC_001139). On Jul 30, 2012 this sequence version replaced NM_001181176.2. ##Genome-Annotation-Data-START## Annotation Provider :: SGD Annotation Status :: Full Annotation Annotation Version :: R64-2-1 URL :: http://www.yeastgenome.org/ ##Genome-Annotation-Data-END## COMPLETENESS: incomplete on both ends.

ATGGCAGCAG GAAAATTGAA AAAGGAACAG CAAAATCAAT CAGCTGAACG AGAAAGCGCT 
GATACGGGTA AAGTTAACGA TGAGGATGAA GAGCATCTAT ACGGAAATAT TGATGACTAT 
AAGCATTTGA TTCAAGATGA GGAATATGAT GACGAGGATG TACCTCATGA TCTACAATTA 
AGCGAAGATG AATATAATTC CGAACGAGAC AGTTCTTTGT TGGCAGAATT CTCGGACTAC 
GGTGAAATAT CTGAAGACGA TGAGGAAGAT TTCATGAACG CGATTAGGGA AGCAAGTAAC 
TTTAAGGTAA AAAAGAAGAA GAAAAATGAC AAAGGTAAAT CTTACGGCCG CCAAAGAAAA 
GAAAGAGTGC TTGATCCAGA GGTAGCGCAA TTACTTTCTC AAGCCAATGA AGCTTTTGTC 
AGGAATGATT TACAAGTGGC CGAAAGATTA TTTAATGAGG TAATTAAAAA AGATGCACGT 
AATTTTGCTG CTTACGAGAC CCTAGGAGAT ATATATCAAT TGCAAGGAAG GCTTAATGAT 
TGTTGTAACT CTTGGTTCCT GGCAGCTCAC CTAAATGCTT CCGATTGGGA ATTCTGGAAG 
ATAGTCGCTA TACTGTCCGC CGATTTAGAT CATGTTAGGC AGGCCATCTA CTGCTTTTCC 
AGGGTAATAA GTTTGAATCC TATGGAATGG GAATCAATTT ATCGCCGGTC TATGTTGTAC 
AAGAAGACAG GACAGTTAGC AAGAGCTTTA GATGGATTTC AAAGGCTTTA CATGTACAAT 
CCTTATGATG CAAACATATT GAGGGAGTTG GCCATTCTTT ATGTTGATTA TGATCGGATT 
GAAGATTCAA TTGAGCTCTA TATGAAAGTG TTTAATGCTA ATGTTGAGAG AAGGGAAGCG 
ATATTAGCTG CTTTAGAAAA TGCCTTAGAT TCTTCAGACG AAGAAAGCGC CGCAGAAGGG 
GAGGATGCAG ACGAAAAGGA ACCCCTTGAA CAAGATGAAG ATAGGCAGAT GTTTCCTGAC 
ATCAACTGGA AAAAAATTGA TGCAAAATAT AAATGCATAC CATTTGACTG GTCTTCTTTG 
AACATTCTCG CGGAACTTTT CTTAAAACTC GCAGTTAGTG AGGTCGATGG TATAAAGACA 
ATTAAAAAAT GTGCACGGTG GATTCAACGC CGCGAAAGCC AGACATTTTG GGATCATGTT 
CCAGATGATT CAGAATTTGA CAATAGAAGG TTTAAAAACA GTACCTTTGA TTCACTACTT 
GCCGCAGAAA AGGAAAAATC GTATAACATT CCAATCGATA TTAGAGTTAG ATTGGGATTG 
TTAAGATTGA ACACAGATAA CCTTGTTGAA GCTCTGAATC ATTTTCAATG CCTGTATGAT 
GAGACTTTCA GTGACGTTGC GGATTTATAT TTTGAGGCTG CAACTGCTTT AACAAGGGCT 
GAAAAGTATA AAGAGGCTAT CGATTTCTTT ACGCCGTTGT TATCCCTTGA GGAATGGCGT 
ACCACTGACG TCTTCAAACC ACTAGCAAGA TGCTACAAGG AAATCGAAAG TTATGAAACG 
GCTAAAGAGT TTTATGAACT AGCTATAAAA TCTGAACCAG ATGATTTAGA TATTCGTGTA 
TCTTTGGCAG AAGTTTACTA TCGCTTAAAT GATCCAGAGA CTTTTAAACA TATGTTAGTT 
GACGTTGTAG AAATGAGGAA ACACCAGGTT GATGAAACAC TGCACAGGAT TTCTAACGAG 
AAAAGTTCTA ACGATACTTC GGATATTTCT AGTAAACCGC TGCTGGAGGA TAGTAAATTT 
AGAACTTTCA GGAAAAAGAA ACGTACACCT TATGATGCAG AAAGAGAGAG AATAGAAAGG 
GAAAGAAGAA TAACGGCAAA AGTCGTTGAT AAATACGAAA AGATGAAAAA ATTTGAACTA 
AATTCAGGTC TCAATGAAGC CAAACAGGCA TCTATATGGA TCAATACAGT TTCTGAATTA 
GTTGATATAT TTTCGAGCGT TAAGAACTTT TTTATGAAAA GTCGATCCAG GAAATTCGTT 
GGAATACTAA GGAGAACTAA AAAGTTTAAC ACCGAGCTTG ATTTCCAAAT TGAAAGGTTA 
TCTAAGTTAG CTGAAGGAGA TAGTGTGTTT GAAGGGCCGC TAATGGAGGA AAGAGTAACC 
TTAACTTCTG CCACAGAATT GAGAGGATTG TCGTATGAGC AGTGGTTTGA GTTATTTATG 
GAACTTTCTT TAGTAATCGC AAAATATCAA AGTGTTGAAG ACGGCCTGAG TGTTGTGGAA 
ACAGCCCAGG AAGTAAACGT GTTCTTCCAG GATCCAGAGA GGGTTAAGAT GATGAAATTT 
GTGAAACTAG CTATTGTTCT TCAAATGGAT GATGAAGAGG AATTGGCCGA AAATTTAAGA 
GGTTTACTAA ATCAATTTCA ATTTAATAGA AAAGTTTTGC AGGTATTTAT GTATTCCCTA 
TGTCGAGGAC CCTCTTCTCT GAATATTTTG AGCTCTACCA TTCAACAAAA ATTTTTCTTA 
AGACAACTGA AAGCTTTTGA CAGTTGTAGA TATAATACCG AGGTAAATGG GCAAGCTTCA 
ATTACGAACA AAGAGGTGTA CAACCCTAAT AAAAAGTCGT CGCCATATTT GTACTATATC 
TATGCTGTTC TTCTCTATTC CAGTAGGGGC TTTCTTTCAG CCCTACAGTA TTTAACTAGG 
TTGGAAGAGG ATATCCCAGA TGACCCGATG GTCAATCTTT TAATGGGTCT GTCACATATT 
CATCGTGCTA TGCAGAGACT AACAGCACAA AGGCATTTTC AAATTTTTCA TGGATTACGT 
TATCTATATC GTTATCATAA AATAAGGAAA AGCCTATATA CGGATTTGGA AAAGCAAGAG 
GCAGACTACA ACTTAGGGCG TGCTTTCCAT TTAATCGGTC TGGTATCGAT TGCAATTGAG 
TACTATAATA GAGTTCTAGA AAACTACGAT GATGGAAAGT TGAAAAAACA TGCTGCCTAT 
AACAGTATTA TAATATATCA GCAAAGCGGA AATGTTGAGT TGGCCGACCA CCTCATGGAG 
AAGTATTTAA GTATTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_011561.3
CDS	1..3078
Translation	MAAGKLKKEQQNQSAERESADTGKVNDEDEEHLYGNIDDYKHLIQDEEYDDEDVPHDLQLSEDEYNSERDSSLLAEFSDYGEISEDDEEDFMNAIREASNFKVKKKKKNDKGKSYGRQRKERVLDPEVAQLLSQANEAFVRNDLQVAERLFNEVIKKDARNFAAYETLGDIYQLQGRLNDCCNSWFLAAHLNASDWEFWKIVAILSADLDHVRQAIYCFSRVISLNPMEWESIYRRSMLYKKTGQLARALDGFQRLYMYNPYDANILRELAILYVDYDRIEDSIELYMKVFNANVERREAILAALENALDSSDEESAAEGEDADEKEPLEQDEDRQMFPDINWKKIDAKYKCIPFDWSSLNILAELFLKLAVSEVDGIKTIKKCARWIQRRESQTFWDHVPDDSEFDNRRFKNSTFDSLLAAEKEKSYNIPIDIRVRLGLLRLNTDNLVEALNHFQCLYDETFSDVADLYFEAATALTRAEKYKEAIDFFTPLLSLEEWRTTDVFKPLARCYKEIESYETAKEFYELAIKSEPDDLDIRVSLAEVYYRLNDPETFKHMLVDVVEMRKHQVDETLHRISNEKSSNDTSDISSKPLLEDSKFRTFRKKKRTPYDAERERIERERRITAKVVDKYEKMKKFELNSGLNEAKQASIWINTVSELVDIFSSVKNFFMKSRSRKFVGILRRTKKFNTELDFQIERLSKLAEGDSVFEGPLMEERVTLTSATELRGLSYEQWFELFMELSLVIAKYQSVEDGLSVVETAQEVNVFFQDPERVKMMKFVKLAIVLQMDDEEELAENLRGLLNQFQFNRKVLQVFMYSLCRGPSSLNILSSTIQQKFFLRQLKAFDSCRYNTEVNGQASITNKEVYNPNKKSSPYLYYIYAVLLYSSRGFLSALQYLTRLEEDIPDDPMVNLLMGLSHIHRAMQRLTAQRHFQIFHGLRYLYRYHKIRKSLYTDLEKQEADYNLGRAFHLIGLVSIAIEYYNRVLENYDDGKLKKHAAYNSIIIYQQSGNVELADHLMEKYLSI

Target ORF information:

RefSeq Version	NM_001181176.3
Organism	Saccharomyces cerevisiae S288C
Definition	Saccharomyces cerevisiae S288C transcription factor TFIIIC subunit TFC4 (TFC4), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001181176.3

ORF Insert Sequence:

ATGGCAGCAG GAAAATTGAA AAAGGAACAG CAAAATCAAT CAGCTGAACG AGAAAGCGCT 
GATACGGGTA AAGTTAACGA TGAGGATGAA GAGCATCTAT ACGGAAATAT TGATGACTAT 
AAGCATTTGA TTCAAGATGA GGAATATGAT GACGAGGATG TACCTCATGA TCTACAATTA 
AGCGAAGATG AATATAATTC CGAACGAGAC AGTTCTTTGT TGGCAGAATT CTCGGACTAC 
GGTGAAATAT CTGAAGACGA TGAGGAAGAT TTCATGAACG CGATTAGGGA AGCAAGTAAC 
TTTAAGGTAA AAAAGAAGAA GAAAAATGAC AAAGGTAAAT CTTACGGCCG CCAAAGAAAA 
GAAAGAGTGC TTGATCCAGA GGTAGCGCAA TTACTTTCTC AAGCCAATGA AGCTTTTGTC 
AGGAATGATT TACAAGTGGC CGAAAGATTA TTTAATGAGG TAATTAAAAA AGATGCACGT 
AATTTTGCTG CTTACGAGAC CCTAGGAGAT ATATATCAAT TGCAAGGAAG GCTTAATGAT 
TGTTGTAACT CTTGGTTCCT GGCAGCTCAC CTAAATGCTT CCGATTGGGA ATTCTGGAAG 
ATAGTCGCTA TACTGTCCGC CGATTTAGAT CATGTTAGGC AGGCCATCTA CTGCTTTTCC 
AGGGTAATAA GTTTGAATCC TATGGAATGG GAATCAATTT ATCGCCGGTC TATGTTGTAC 
AAGAAGACAG GACAGTTAGC AAGAGCTTTA GATGGATTTC AAAGGCTTTA CATGTACAAT 
CCTTATGATG CAAACATATT GAGGGAGTTG GCCATTCTTT ATGTTGATTA TGATCGGATT 
GAAGATTCAA TTGAGCTCTA TATGAAAGTG TTTAATGCTA ATGTTGAGAG AAGGGAAGCG 
ATATTAGCTG CTTTAGAAAA TGCCTTAGAT TCTTCAGACG AAGAAAGCGC CGCAGAAGGG 
GAGGATGCAG ACGAAAAGGA ACCCCTTGAA CAAGATGAAG ATAGGCAGAT GTTTCCTGAC 
ATCAACTGGA AAAAAATTGA TGCAAAATAT AAATGCATAC CATTTGACTG GTCTTCTTTG 
AACATTCTCG CGGAACTTTT CTTAAAACTC GCAGTTAGTG AGGTCGATGG TATAAAGACA 
ATTAAAAAAT GTGCACGGTG GATTCAACGC CGCGAAAGCC AGACATTTTG GGATCATGTT 
CCAGATGATT CAGAATTTGA CAATAGAAGG TTTAAAAACA GTACCTTTGA TTCACTACTT 
GCCGCAGAAA AGGAAAAATC GTATAACATT CCAATCGATA TTAGAGTTAG ATTGGGATTG 
TTAAGATTGA ACACAGATAA CCTTGTTGAA GCTCTGAATC ATTTTCAATG CCTGTATGAT 
GAGACTTTCA GTGACGTTGC GGATTTATAT TTTGAGGCTG CAACTGCTTT AACAAGGGCT 
GAAAAGTATA AAGAGGCTAT CGATTTCTTT ACGCCGTTGT TATCCCTTGA GGAATGGCGT 
ACCACTGACG TCTTCAAACC ACTAGCAAGA TGCTACAAGG AAATCGAAAG TTATGAAACG 
GCTAAAGAGT TTTATGAACT AGCTATAAAA TCTGAACCAG ATGATTTAGA TATTCGTGTA 
TCTTTGGCAG AAGTTTACTA TCGCTTAAAT GATCCAGAGA CTTTTAAACA TATGTTAGTT 
GACGTTGTAG AAATGAGGAA ACACCAGGTT GATGAAACAC TGCACAGGAT TTCTAACGAG 
AAAAGTTCTA ACGATACTTC GGATATTTCT AGTAAACCGC TGCTGGAGGA TAGTAAATTT 
AGAACTTTCA GGAAAAAGAA ACGTACACCT TATGATGCAG AAAGAGAGAG AATAGAAAGG 
GAAAGAAGAA TAACGGCAAA AGTCGTTGAT AAATACGAAA AGATGAAAAA ATTTGAACTA 
AATTCAGGTC TCAATGAAGC CAAACAGGCA TCTATATGGA TCAATACAGT TTCTGAATTA 
GTTGATATAT TTTCGAGCGT TAAGAACTTT TTTATGAAAA GTCGATCCAG GAAATTCGTT 
GGAATACTAA GGAGAACTAA AAAGTTTAAC ACCGAGCTTG ATTTCCAAAT TGAAAGGTTA 
TCTAAGTTAG CTGAAGGAGA TAGTGTGTTT GAAGGGCCGC TAATGGAGGA AAGAGTAACC 
TTAACTTCTG CCACAGAATT GAGAGGATTG TCGTATGAGC AGTGGTTTGA GTTATTTATG 
GAACTTTCTT TAGTAATCGC AAAATATCAA AGTGTTGAAG ACGGCCTGAG TGTTGTGGAA 
ACAGCCCAGG AAGTAAACGT GTTCTTCCAG GATCCAGAGA GGGTTAAGAT GATGAAATTT 
GTGAAACTAG CTATTGTTCT TCAAATGGAT GATGAAGAGG AATTGGCCGA AAATTTAAGA 
GGTTTACTAA ATCAATTTCA ATTTAATAGA AAAGTTTTGC AGGTATTTAT GTATTCCCTA 
TGTCGAGGAC CCTCTTCTCT GAATATTTTG AGCTCTACCA TTCAACAAAA ATTTTTCTTA 
AGACAACTGA AAGCTTTTGA CAGTTGTAGA TATAATACCG AGGTAAATGG GCAAGCTTCA 
ATTACGAACA AAGAGGTGTA CAACCCTAAT AAAAAGTCGT CGCCATATTT GTACTATATC 
TATGCTGTTC TTCTCTATTC CAGTAGGGGC TTTCTTTCAG CCCTACAGTA TTTAACTAGG 
TTGGAAGAGG ATATCCCAGA TGACCCGATG GTCAATCTTT TAATGGGTCT GTCACATATT 
CATCGTGCTA TGCAGAGACT AACAGCACAA AGGCATTTTC AAATTTTTCA TGGATTACGT 
TATCTATATC GTTATCATAA AATAAGGAAA AGCCTATATA CGGATTTGGA AAAGCAAGAG 
GCAGACTACA ACTTAGGGCG TGCTTTCCAT TTAATCGGTC TGGTATCGAT TGCAATTGAG 
TACTATAATA GAGTTCTAGA AAACTACGAT GATGGAAAGT TGAAAAAACA TGCTGCCTAT 
AACAGTATTA TAATATATCA GCAAAGCGGA AATGTTGAGT TGGCCGACCA CCTCATGGAG 
AAGTATTTAA GTATTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The nucleotide sequence of Saccharomyces cerevisiae chromosome VII.
Nature387(6632 Suppl)81-4(1997 May)
Tettelin H,Agostoni Carbone ML,Albermann K,Albers M,Arroyo J,Backes U,Barreiros T,Bertani I,Bjourson AJ,Br?ckner M,Bruschi CV,Carignani G,Castagnoli L,Cerdan E,Clemente ML,Coblenz A,Coglievina M,Coissac E,Defoor E,Del Bino S,Delius H,Delneri D,de Wergifosse P,Dujon B,Kleine K

Life with 6000 genes.
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)
Goffeau A,Barrell BG,Bussey H,Davis RW,Dujon B,Feldmann H,Galibert F,Hoheisel JD,Jacq C,Johnston M,Louis EJ,Mewes HW,Murakami Y,Philippsen P,Tettelin H,Oliver SG

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TFC4 cDNA ORF clone, Saccharomyces cerevisiae S288C

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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