NUT1 cDNA ORF clone, Saccharomyces cerevisiae S288C

Gene
Clones
gRNAs

Summary

Gene Symbol	NUT1
Entrez Gene ID	852726
Full Name	Nut1p
Synonyms	MED5,SSX4
Gene Type	protein-coding
Organism	Saccharomyces cerevisiae S288C

mRNA and Protein(s)

mRNA	Protein	Name
NM_001181016.1	NP_011364.1	Nut1p

Pathways from BioSystems

Homologies

Kluyveromyces lactis	KLLA0E08977g	XP_454354.1
Eremothecium gossypii	AGOS_AGR380C	NP_987046.1
Saccharomyces cerevisiae (baker's yeast)	NUT1	NP_011364.1

Gene Ontology
Bibliography

Related articles in PubMed

The nucleotide sequence of Saccharomyces cerevisiae chromosome VII.
Tettelin H, Agostoni Carbone ML, Albermann K, Albers M, Arroyo J, Backes U, Barreiros T, Bertani I, Bjourson AJ, Br?ckner M, Bruschi CV, Carignani G, Castagnoli L, Cerdan E, Clemente ML, Coblenz A, Coglievina M, Coissac E, Defoor E, Del Bino S, Delius H, Delneri D, de Wergifosse P, Dujon B, Kleine K
Nature387(6632 Suppl)81-4(1997 May)

Life with 6000 genes.
Goffeau A, Barrell BG, Bussey H, Davis RW, Dujon B, Feldmann H, Galibert F, Hoheisel JD, Jacq C, Johnston M, Louis EJ, Mewes HW, Murakami Y, Philippsen P, Tettelin H, Oliver SG
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The analysis has identified the Med5 subunit of Mediator as a target for histone tail interactions and suggests that the previously observed effect of med5 mutations on telomeric heterochromatin and silencing is direct.
Title: Med5(Nut1) and Med17(Srb4) are direct targets of mediator histone H4 tail interactions.

Med5 has a role in coordinating nuclear and mitochondrial gene transcription
Title: The structural and functional role of Med5 in the yeast Mediator tail module.

The following NUT1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the NUT1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OSi02099	NM_001181016.1 Latest version!	Saccharomyces cerevisiae S288C Nut1p (NUT1), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

NUT1 ( NM_001181016.1 ) cDNA ORF clone, Saccharomyces cerevisiae S288C -> NP_011364.1 Saccharomyces cerevisiae S288C Nut1p (NUT1), partial mRNA.

Price & Availability↑

CloneID	OSi02099
Clone ID Related Accession (Same CDS sequence)	NM_001181016.1
Accession Version	NM_001181016.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3399bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-31
Organism	Saccharomyces cerevisiae S288C
Product	Nut1p
Comment	Comment: REVIEWED REFSEQ: This record has been curated by SGD. This record is derived from an annotated genomic sequence (NC_001139). ##Genome-Annotation-Data-START## Annotation Provider :: SGD Annotation Status :: Full Annotation Annotation Version :: R64-2-1 URL :: http://www.yeastgenome.org/ ##Genome-Annotation-Data-END## COMPLETENESS: incomplete on both ends.

ATGGAAAAAG AATCAGTATA CAACTTAGCA CTAAAATGTG CTGAGCGGCA ATTGACCTCC 
ATGGAATTTT CGAACCTTTA CAAGGAATTC TTTAATGAAA AGTTCCCGTC TTTGATTCAA 
GAGGAAGAAG AAGATACAAC AACAACTGCA AATATAAATG AGGTCAAGAA AGCGTCCGAT 
TTGGTGGATA CACCCAGCAA CAACACGGCT GCTACTGCAG ATACTACACA CTTACACGAG 
GCTCTAGATA TTGTTTGCTC CGATTTTGTT AAAATATTAA ACCTGGAGAA GCCTCTTATC 
TTGGCAGATT ATATCGTTGA GGTATTGCTT GTGAACTACA ACTCAGATAT GATAAAATGC 
TTTCTGCCTA AATTGAATTC CGTTAGGAAC TCCTTGCTAT TGGCCCATTT TTTCTCCAAA 
TCTTGTTCAT TCTTTGCCAA ATTATCAGAT ACTTTGATAA TTGATCAAGT ACGCAAGGAT 
TTGGGCAACG TCATAGTACC GAATATCTTA AGTCTAGACA TGAATAGTAT GAATAAAGAA 
TTGATTGCCA TAGTATCAAA GCTTTTGCAA ACAACTTTAA AACTCTCTCC TTCACCAATT 
CTTTTAACTT CTGCCGGCTG TAAAAATGGG TCTTTTACGC TACTAAATCA GTTGTCTCAA 
ACCAACAAGT TACTTTTTAA AAGGGTTTCT CAGACTTTTG AAGCAAAATT ACATTTCAAA 
GATACCAAGC CATTTTTAAA CAAAGATTCC ACTAACGAAT TCGTAGGTTC TCCTTCATTA 
ACCTCCCCTC AATATATCCC AAGTCCACTT TCTTCAACAA AACCTCCAGG ATCAGTCAAT 
TCTGCTGCCA AATACAAAGA CATGAAGCTT CTTCGTTACT ATAAAAACAT TTGGCTGAAC 
AATAAAATTA TCAATTGGGA AATATCGAAC CCAGACTTCC TATCAAAATA TTCTGCGATT 
ACTTCTTCTA TATTTCAAGA GAGCTTTAAT TCAGTTCAAA ACTTGGACCA GTTACTCACA 
GACTTGATTG AGACATCGTT TACTTGTTTT GCTCAATTCG TTAGCAATAA GCAATATCAT 
CAGGCAAATT CTAATTTGAC TCTGTTAGAA AGAAAGTGGG TCATATTTAT AACAAAACAT 
CTACCATTAT TAATCCTGGA AAACTCTTCA AGAAGTCCTC GTGTAGTCAC GAATGCTCTG 
GATAATATTG ACGAAAAAGT CGTTAAAGCA ATCAGAATAT ACTTCACCGA AAAAGATGAT 
AACAAAACCA ATAATGAAGA TCTATTTGAT GATTATCCAT CTACAAGCTT GGATATAAGA 
CACGATTTTA TCAAAGGGTT GATTATGTTA AATTTACAGC CTGCGTCCGT TATCAACAAT 
TACTTAAGAG AGGACCAAAT GATCGACACC AGTATTTTAC CTACCCGTGA TGATTTGTTT 
GTTCGTAATT TGCAAGGCAT TCAAGAAGTC GTCCATAATA CCAACAGTTT CATCATTTCA 
TCCTTAGATA CTTTAGAGTT AGAATCGATA ACCGAATCGA TCACACATGA TTCGAGTAAC 
GGACTATTTC AAGTATTACA CAATTTTGAA TCTGTAGCTC CGACAAAACA ACGTGAGATA 
GTAAAAGCCT TTTTATCTAT ATTTGAAGAT GCAATAAAAG AATTGAATTA TAATAGAATA 
GCCAAGATAT GTGCTCTTTT GTTTTTCAAC TTTTCACATT CATTGACCAC AATTCTATCA 
TTTTCTTCGC CAGCAGCACT AATGAAAACA TTGATAAAAT TCGTTGATTT GTCTAGAAAT 
GGCAGAAATG GCTCAAACGG TAATGATGAA AGTTCAGAAT ATGAAACGAT AAATATATCT 
TTGTCATTTT CTTGGGCAAT TTTACTGATA ATCAACCTAA CACAAACATA TGGCATATCT 
GTGGTGGATG TGGCACTTAA GTATCCTGAA TTGAGCATAA AAAATTCCTT TATAATCAAT 
TTCATTTCAA ATCTACCTAA TGTCTCAGAT AAGTACTATT TGGAAGAATC AAATGTGAAT 
GATTCAGATA TGCTAACAAA GTCTCATAAT ACCGTTCAAA GCTGGCTTTG TGATCTTTTT 
GTTAATGGGT CCATAACAGA TCAATTGATC CAAAATATTG AAACAAGGCA ATTGGCTAAT 
CTCATACCAT TTATTGTTAA ACAGGTTTTG CTATCTGTTG AAATAGGCGT TTTAACGGAT 
ATTTCAAGTC TGATAGGTGG CTTCGAATAT TTCTTACAAC CTTTACTATT AGTTGGGCTA 
ATCAAAACTT TTTATTGGCT AGAACAATTT CTTTCTTGCG TCAAGAACGA CACAATATCT 
GAAGATATTT TACAAGGGAT ATTCAATTTA TTGAATACAC TTTTCAACCC AGTAACTCTT 
AACGAGGATT CGAAAGCGTT CCACACGGCT GTACTGAGGT TGAATGCAAT TCCACTACTC 
AAAGTTCTCC GCAAATTTAG AGTTCAGAGC CAGTCAAATT ATGGTATCTA CTCTTCTGAT 
GCCCAAGGTG ATCCTAACTT AGAACCTTTG ATAGCCAAAT TAGTTGCCGT TCTTAATGTA 
TCACCCGTTT ATGACGTCGA TCCAAGAATT ATCAATTCAG AGAACGATTA TTCTAGAAAG 
CAGTTGGGTT ACGGTAAATT CCTAATTTTG AACGAAAACC CGATTAACAA GATAATGACG 
AACCAAATTA ACTCATTCTG GAGTCTCCAC AGCAGCACAT ATTATAACTT AGATTATCTA 
TTTGAGTTAA TTGAATTGGT TACCCCAAAA AGTTTCCTTT TTGATGTTTT GAAAACATTA 
GAATATAAAC TAGCCACTTA TGGGGTCCCT GGTTCTGAAA ATAAGAGGGG ATCATTGGAT 
TCAGAGCATG TTTTCGATTA TTTCTTCTAC TTTCTTGTAC TATATGATGT GAAAACCGCG 
GAGGAAGCCA GTCAACTAAT AGAATATATG GAGAACGACG CAAAAAAAAG TAAGGGGGAC 
GTTGATATAA AGGGAGAAGA CTTACATGAG AAAAATGACT CAGCTGAGGT GAGGCAAGAA 
ACTCAACCAA AAGCTGAAGC TACCCAAGAC GATGATTTCG ATATGCTTTT TGGCGAGAAT 
GATACAAGCA CTCAAGCTTA CGAAGAAGAA GAAGAGAATG AGGATAACGA CGGCAATAAT 
AGAACAAACA ATGTACCAAT GATAAAAGCA GAAGAAACTC CTAGTAAAAC TAATAAGATA 
TCCATTTTAA AGAGACATTC GTTTGCGGTA CTTCTGCATG AAAGAAAATT ATTGAATGAT 
TTAGCTTTGG AAAACGGCGA AATAACCAAG ACAGAAAATG AGAAATTTAT CAGTTATCAC 
GATAAGTACT TGTGTATGCT GAAAACATGC GTATTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_011364.1
CDS	1..3399
Translation	MEKESVYNLALKCAERQLTSMEFSNLYKEFFNEKFPSLIQEEEEDTTTTANINEVKKASDLVDTPSNNTAATADTTHLHEALDIVCSDFVKILNLEKPLILADYIVEVLLVNYNSDMIKCFLPKLNSVRNSLLLAHFFSKSCSFFAKLSDTLIIDQVRKDLGNVIVPNILSLDMNSMNKELIAIVSKLLQTTLKLSPSPILLTSAGCKNGSFTLLNQLSQTNKLLFKRVSQTFEAKLHFKDTKPFLNKDSTNEFVGSPSLTSPQYIPSPLSSTKPPGSVNSAAKYKDMKLLRYYKNIWLNNKIINWEISNPDFLSKYSAITSSIFQESFNSVQNLDQLLTDLIETSFTCFAQFVSNKQYHQANSNLTLLERKWVIFITKHLPLLILENSSRSPRVVTNALDNIDEKVVKAIRIYFTEKDDNKTNNEDLFDDYPSTSLDIRHDFIKGLIMLNLQPASVINNYLREDQMIDTSILPTRDDLFVRNLQGIQEVVHNTNSFIISSLDTLELESITESITHDSSNGLFQVLHNFESVAPTKQREIVKAFLSIFEDAIKELNYNRIAKICALLFFNFSHSLTTILSFSSPAALMKTLIKFVDLSRNGRNGSNGNDESSEYETINISLSFSWAILLIINLTQTYGISVVDVALKYPELSIKNSFIINFISNLPNVSDKYYLEESNVNDSDMLTKSHNTVQSWLCDLFVNGSITDQLIQNIETRQLANLIPFIVKQVLLSVEIGVLTDISSLIGGFEYFLQPLLLVGLIKTFYWLEQFLSCVKNDTISEDILQGIFNLLNTLFNPVTLNEDSKAFHTAVLRLNAIPLLKVLRKFRVQSQSNYGIYSSDAQGDPNLEPLIAKLVAVLNVSPVYDVDPRIINSENDYSRKQLGYGKFLILNENPINKIMTNQINSFWSLHSSTYYNLDYLFELIELVTPKSFLFDVLKTLEYKLATYGVPGSENKRGSLDSEHVFDYFFYFLVLYDVKTAEEASQLIEYMENDAKKSKGDVDIKGEDLHEKNDSAEVRQETQPKAEATQDDDFDMLFGENDTSTQAYEEEEENEDNDGNNRTNNVPMIKAEETPSKTNKISILKRHSFAVLLHERKLLNDLALENGEITKTENEKFISYHDKYLCMLKTCVF

Target ORF information:

RefSeq Version	NM_001181016.1
Organism	Saccharomyces cerevisiae S288C
Definition	Saccharomyces cerevisiae S288C Nut1p (NUT1), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001181016.1

ORF Insert Sequence:

ATGGAAAAAG AATCAGTATA CAACTTAGCA CTAAAATGTG CTGAGCGGCA ATTGACCTCC 
ATGGAATTTT CGAACCTTTA CAAGGAATTC TTTAATGAAA AGTTCCCGTC TTTGATTCAA 
GAGGAAGAAG AAGATACAAC AACAACTGCA AATATAAATG AGGTCAAGAA AGCGTCCGAT 
TTGGTGGATA CACCCAGCAA CAACACGGCT GCTACTGCAG ATACTACACA CTTACACGAG 
GCTCTAGATA TTGTTTGCTC CGATTTTGTT AAAATATTAA ACCTGGAGAA GCCTCTTATC 
TTGGCAGATT ATATCGTTGA GGTATTGCTT GTGAACTACA ACTCAGATAT GATAAAATGC 
TTTCTGCCTA AATTGAATTC CGTTAGGAAC TCCTTGCTAT TGGCCCATTT TTTCTCCAAA 
TCTTGTTCAT TCTTTGCCAA ATTATCAGAT ACTTTGATAA TTGATCAAGT ACGCAAGGAT 
TTGGGCAACG TCATAGTACC GAATATCTTA AGTCTAGACA TGAATAGTAT GAATAAAGAA 
TTGATTGCCA TAGTATCAAA GCTTTTGCAA ACAACTTTAA AACTCTCTCC TTCACCAATT 
CTTTTAACTT CTGCCGGCTG TAAAAATGGG TCTTTTACGC TACTAAATCA GTTGTCTCAA 
ACCAACAAGT TACTTTTTAA AAGGGTTTCT CAGACTTTTG AAGCAAAATT ACATTTCAAA 
GATACCAAGC CATTTTTAAA CAAAGATTCC ACTAACGAAT TCGTAGGTTC TCCTTCATTA 
ACCTCCCCTC AATATATCCC AAGTCCACTT TCTTCAACAA AACCTCCAGG ATCAGTCAAT 
TCTGCTGCCA AATACAAAGA CATGAAGCTT CTTCGTTACT ATAAAAACAT TTGGCTGAAC 
AATAAAATTA TCAATTGGGA AATATCGAAC CCAGACTTCC TATCAAAATA TTCTGCGATT 
ACTTCTTCTA TATTTCAAGA GAGCTTTAAT TCAGTTCAAA ACTTGGACCA GTTACTCACA 
GACTTGATTG AGACATCGTT TACTTGTTTT GCTCAATTCG TTAGCAATAA GCAATATCAT 
CAGGCAAATT CTAATTTGAC TCTGTTAGAA AGAAAGTGGG TCATATTTAT AACAAAACAT 
CTACCATTAT TAATCCTGGA AAACTCTTCA AGAAGTCCTC GTGTAGTCAC GAATGCTCTG 
GATAATATTG ACGAAAAAGT CGTTAAAGCA ATCAGAATAT ACTTCACCGA AAAAGATGAT 
AACAAAACCA ATAATGAAGA TCTATTTGAT GATTATCCAT CTACAAGCTT GGATATAAGA 
CACGATTTTA TCAAAGGGTT GATTATGTTA AATTTACAGC CTGCGTCCGT TATCAACAAT 
TACTTAAGAG AGGACCAAAT GATCGACACC AGTATTTTAC CTACCCGTGA TGATTTGTTT 
GTTCGTAATT TGCAAGGCAT TCAAGAAGTC GTCCATAATA CCAACAGTTT CATCATTTCA 
TCCTTAGATA CTTTAGAGTT AGAATCGATA ACCGAATCGA TCACACATGA TTCGAGTAAC 
GGACTATTTC AAGTATTACA CAATTTTGAA TCTGTAGCTC CGACAAAACA ACGTGAGATA 
GTAAAAGCCT TTTTATCTAT ATTTGAAGAT GCAATAAAAG AATTGAATTA TAATAGAATA 
GCCAAGATAT GTGCTCTTTT GTTTTTCAAC TTTTCACATT CATTGACCAC AATTCTATCA 
TTTTCTTCGC CAGCAGCACT AATGAAAACA TTGATAAAAT TCGTTGATTT GTCTAGAAAT 
GGCAGAAATG GCTCAAACGG TAATGATGAA AGTTCAGAAT ATGAAACGAT AAATATATCT 
TTGTCATTTT CTTGGGCAAT TTTACTGATA ATCAACCTAA CACAAACATA TGGCATATCT 
GTGGTGGATG TGGCACTTAA GTATCCTGAA TTGAGCATAA AAAATTCCTT TATAATCAAT 
TTCATTTCAA ATCTACCTAA TGTCTCAGAT AAGTACTATT TGGAAGAATC AAATGTGAAT 
GATTCAGATA TGCTAACAAA GTCTCATAAT ACCGTTCAAA GCTGGCTTTG TGATCTTTTT 
GTTAATGGGT CCATAACAGA TCAATTGATC CAAAATATTG AAACAAGGCA ATTGGCTAAT 
CTCATACCAT TTATTGTTAA ACAGGTTTTG CTATCTGTTG AAATAGGCGT TTTAACGGAT 
ATTTCAAGTC TGATAGGTGG CTTCGAATAT TTCTTACAAC CTTTACTATT AGTTGGGCTA 
ATCAAAACTT TTTATTGGCT AGAACAATTT CTTTCTTGCG TCAAGAACGA CACAATATCT 
GAAGATATTT TACAAGGGAT ATTCAATTTA TTGAATACAC TTTTCAACCC AGTAACTCTT 
AACGAGGATT CGAAAGCGTT CCACACGGCT GTACTGAGGT TGAATGCAAT TCCACTACTC 
AAAGTTCTCC GCAAATTTAG AGTTCAGAGC CAGTCAAATT ATGGTATCTA CTCTTCTGAT 
GCCCAAGGTG ATCCTAACTT AGAACCTTTG ATAGCCAAAT TAGTTGCCGT TCTTAATGTA 
TCACCCGTTT ATGACGTCGA TCCAAGAATT ATCAATTCAG AGAACGATTA TTCTAGAAAG 
CAGTTGGGTT ACGGTAAATT CCTAATTTTG AACGAAAACC CGATTAACAA GATAATGACG 
AACCAAATTA ACTCATTCTG GAGTCTCCAC AGCAGCACAT ATTATAACTT AGATTATCTA 
TTTGAGTTAA TTGAATTGGT TACCCCAAAA AGTTTCCTTT TTGATGTTTT GAAAACATTA 
GAATATAAAC TAGCCACTTA TGGGGTCCCT GGTTCTGAAA ATAAGAGGGG ATCATTGGAT 
TCAGAGCATG TTTTCGATTA TTTCTTCTAC TTTCTTGTAC TATATGATGT GAAAACCGCG 
GAGGAAGCCA GTCAACTAAT AGAATATATG GAGAACGACG CAAAAAAAAG TAAGGGGGAC 
GTTGATATAA AGGGAGAAGA CTTACATGAG AAAAATGACT CAGCTGAGGT GAGGCAAGAA 
ACTCAACCAA AAGCTGAAGC TACCCAAGAC GATGATTTCG ATATGCTTTT TGGCGAGAAT 
GATACAAGCA CTCAAGCTTA CGAAGAAGAA GAAGAGAATG AGGATAACGA CGGCAATAAT 
AGAACAAACA ATGTACCAAT GATAAAAGCA GAAGAAACTC CTAGTAAAAC TAATAAGATA 
TCCATTTTAA AGAGACATTC GTTTGCGGTA CTTCTGCATG AAAGAAAATT ATTGAATGAT 
TTAGCTTTGG AAAACGGCGA AATAACCAAG ACAGAAAATG AGAAATTTAT CAGTTATCAC 
GATAAGTACT TGTGTATGCT GAAAACATGC GTATTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The nucleotide sequence of Saccharomyces cerevisiae chromosome VII.
Nature387(6632 Suppl)81-4(1997 May)
Tettelin H,Agostoni Carbone ML,Albermann K,Albers M,Arroyo J,Backes U,Barreiros T,Bertani I,Bjourson AJ,Br?ckner M,Bruschi CV,Carignani G,Castagnoli L,Cerdan E,Clemente ML,Coblenz A,Coglievina M,Coissac E,Defoor E,Del Bino S,Delius H,Delneri D,de Wergifosse P,Dujon B,Kleine K

Life with 6000 genes.
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)
Goffeau A,Barrell BG,Bussey H,Davis RW,Dujon B,Feldmann H,Galibert F,Hoheisel JD,Jacq C,Johnston M,Louis EJ,Mewes HW,Murakami Y,Philippsen P,Tettelin H,Oliver SG

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NUT1 cDNA ORF clone, Saccharomyces cerevisiae S288C

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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