ORF » Species Summary » Saccharomyces cerevisiae S288C » IME4 cDNA ORF clone

IME4 cDNA ORF clone, Saccharomyces cerevisiae S288C

The following IME4 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the IME4 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OSi02063 NM_001181057.3
Latest version!		 Saccharomyces cerevisiae S288C mRNA (N6-adenosine)-methyltransferase (IME4), partial mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $342.30
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    IME4 ( NM_001181057.3 ) cDNA ORF clone, Saccharomyces cerevisiae S288C -> NP_011323.3 Saccharomyces cerevisiae S288C mRNA (N6-adenosine)-methyltransferase (IME4), partial mRNA.
    Price & Availability↑
    CloneID OSi02063
    Clone ID Related Accession (Same CDS sequence) NM_001181057.3
    Accession Version NM_001181057.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1803bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-10-31
    Organism Saccharomyces cerevisiae S288C
    Product mRNA (N6-adenosine)-methyltransferase
    Comment Comment: REVIEWED REFSEQ: This record has been curated by SGD. This record is derived from an annotated genomic sequence (NC_001139). On Jul 30, 2012 this sequence version replaced NM_001181057.2. ##Genome-Annotation-Data-START## Annotation Provider :: SGD Annotation Status :: Full Annotation Annotation Version :: R64-2-1 URL :: http://www.yeastgenome.org/ ##Genome-Annotation-Data-END## COMPLETENESS: incomplete on both ends. 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    ATGATTAACG ATAAACTAGT ACATTTTCTG ATCCAGAATT ATGATGACAT CCTAAGAGCA 
    CCGCTTTCTG GACAGCTCAA AGATGTGTAT TCACTGTACA TCAGTGGCGG ATACGATGAT 
    GAGATGCAGA AGTTGAGAAA CGATAAAGAC GAGGTCTTAC AGTTTGAACA GTTTTGGAAT 
    GATCTTCAGG ATATTATATT TGCAACGCCG CAGTCCATTC AATTTGACCA GAATCTATTA 
    GTGGCAGATA GGCCAGAAAA AATTGTTTAC CTGGACGTTT TTTCCTTGAA AATTCTCTAT 
    AACAAGTTCC ATGCCTTCTA CTATACATTA AAATCGTCCA GTTCTTCATG TGAAGAAAAA 
    GTGTCGAGTT TAACAACAAA ACCGGAAGCT GATTCGGAAA AAGACCAACT GCTGGGTAGA 
    CTGCTTGGAG TCCTGAACTG GGACGTTAAC GTCAGCAACC AGGGGTTACC ACGTGAACAA 
    CTGAGCAATC GCTTACAAAA TTTATTGAGG GAAAAACCAT CATCTTTTCA GCTTGCTAAA 
    GAAAGAGCCA AATACACGAC CGAGGTCATT GAATACATCC CGATATGTAG TGACTATTCG 
    CATGCATCTC TATTGTCCAC CTCGGTGTAC ATTGTCAATA ACAAAATCGT TTCTTTACAA 
    TGGTCCAAAA TATCCGCATG TCAGGAAAAT CACCCAGGAT TAATTGAGTG CATTCAATCA 
    AAAATTCACT TTATCCCTAA TATCAAACCA CAAACTGATA TTTCGTTAGG AGATTGTTCC 
    TATTTGGATA CCTGTCATAA ATTAAATACG TGTCGATACA TTCATTACCT GCAATATATT 
    CCGTCATGCT TGCAAGAGCG AGCTGATCGT GAAACTGCGA GTGAAAATAA AAGAATACGG 
    TCAAACGTTT CCATTCCATT TTATACTCTG GGCAATTGCT CCGCACATTG TATCAAAAAG 
    GCTTTACCTG CACAGTGGAT CCGTTGCGAT GTGCGGAAAT TTGATTTTAG GGTTCTTGGA 
    AAGTTTTCGG TAGTTATTGC AGATCCTGCA TGGAATATCC ATATGAACCT ACCATACGGT 
    ACTTGCAACG ATATTGAACT ATTAGGTTTG CCTTTGCACG AACTACAAGA TGAAGGTATT 
    ATTTTTCTTT GGGTGACGGG TAGAGCTATA GAATTGGGTA AAGAGTCTTT GAATAACTGG 
    GGTTACAACG TGATAAATGA AGTTTCATGG ATAAAGACAA ACCAACTGGG TAGAACAATT 
    GTTACAGGTC GTACGGGTCA TTGGTTAAAT CATTCTAAGG AGCATTTATT GGTGGGCTTA 
    AAGGGTAATC CAAAGTGGAT CAACAAACAT ATTGACGTCG ATTTGATCGT ATCAATGACC 
    AGAGAGACTA GTAGAAAGCC TGATGAACTG TATGGTATAG CAGAACGGTT GGCAGGCACC 
    CATGCCAGAA AACTAGAGAT ATTCGGAAGG GATCACAATA CGCGGCCTGG CTGGTTTACC 
    ATCGGAAACC AGTTGACAGG AAATTGCATT TACGAAATGG ATGTCGAGAG GAAGTACCAG 
    GAGTTTATGA AGAGCAAAAC TGGAACCAGC CACACTGGTA CTAAAAAAAT CGACAAAAAG 
    CAACCTTCAA AATTACAGCA GCAGCATCAG CAGCAGTATT GGAATAATAT GGATATGGGG 
    AGTGGTAAGT ATTATGCTGA GGCCAAGCAG AATCCTATGA ATCAAAAACA TACTCCCTTT 
    GAATCCAAAC AGCAACAAAA ACAACAATTC CAAACACTAA ATAACCTATA TTTTGCTCAG 
    TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_011323.3
    CDS1..1803
    Translation

    Target ORF information:

    RefSeq Version NM_001181057.3
    Organism Saccharomyces cerevisiae S288C
    Definition Saccharomyces cerevisiae S288C mRNA (N6-adenosine)-methyltransferase (IME4), partial mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 NM_001181057.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    ATGATTAACG ATAAACTAGT ACATTTTCTG ATCCAGAATT ATGATGACAT CCTAAGAGCA 
    CCGCTTTCTG GACAGCTCAA AGATGTGTAT TCACTGTACA TCAGTGGCGG ATACGATGAT 
    GAGATGCAGA AGTTGAGAAA CGATAAAGAC GAGGTCTTAC AGTTTGAACA GTTTTGGAAT 
    GATCTTCAGG ATATTATATT TGCAACGCCG CAGTCCATTC AATTTGACCA GAATCTATTA 
    GTGGCAGATA GGCCAGAAAA AATTGTTTAC CTGGACGTTT TTTCCTTGAA AATTCTCTAT 
    AACAAGTTCC ATGCCTTCTA CTATACATTA AAATCGTCCA GTTCTTCATG TGAAGAAAAA 
    GTGTCGAGTT TAACAACAAA ACCGGAAGCT GATTCGGAAA AAGACCAACT GCTGGGTAGA 
    CTGCTTGGAG TCCTGAACTG GGACGTTAAC GTCAGCAACC AGGGGTTACC ACGTGAACAA 
    CTGAGCAATC GCTTACAAAA TTTATTGAGG GAAAAACCAT CATCTTTTCA GCTTGCTAAA 
    GAAAGAGCCA AATACACGAC CGAGGTCATT GAATACATCC CGATATGTAG TGACTATTCG 
    CATGCATCTC TATTGTCCAC CTCGGTGTAC ATTGTCAATA ACAAAATCGT TTCTTTACAA 
    TGGTCCAAAA TATCCGCATG TCAGGAAAAT CACCCAGGAT TAATTGAGTG CATTCAATCA 
    AAAATTCACT TTATCCCTAA TATCAAACCA CAAACTGATA TTTCGTTAGG AGATTGTTCC 
    TATTTGGATA CCTGTCATAA ATTAAATACG TGTCGATACA TTCATTACCT GCAATATATT 
    CCGTCATGCT TGCAAGAGCG AGCTGATCGT GAAACTGCGA GTGAAAATAA AAGAATACGG 
    TCAAACGTTT CCATTCCATT TTATACTCTG GGCAATTGCT CCGCACATTG TATCAAAAAG 
    GCTTTACCTG CACAGTGGAT CCGTTGCGAT GTGCGGAAAT TTGATTTTAG GGTTCTTGGA 
    AAGTTTTCGG TAGTTATTGC AGATCCTGCA TGGAATATCC ATATGAACCT ACCATACGGT 
    ACTTGCAACG ATATTGAACT ATTAGGTTTG CCTTTGCACG AACTACAAGA TGAAGGTATT 
    ATTTTTCTTT GGGTGACGGG TAGAGCTATA GAATTGGGTA AAGAGTCTTT GAATAACTGG 
    GGTTACAACG TGATAAATGA AGTTTCATGG ATAAAGACAA ACCAACTGGG TAGAACAATT 
    GTTACAGGTC GTACGGGTCA TTGGTTAAAT CATTCTAAGG AGCATTTATT GGTGGGCTTA 
    AAGGGTAATC CAAAGTGGAT CAACAAACAT ATTGACGTCG ATTTGATCGT ATCAATGACC 
    AGAGAGACTA GTAGAAAGCC TGATGAACTG TATGGTATAG CAGAACGGTT GGCAGGCACC 
    CATGCCAGAA AACTAGAGAT ATTCGGAAGG GATCACAATA CGCGGCCTGG CTGGTTTACC 
    ATCGGAAACC AGTTGACAGG AAATTGCATT TACGAAATGG ATGTCGAGAG GAAGTACCAG 
    GAGTTTATGA AGAGCAAAAC TGGAACCAGC CACACTGGTA CTAAAAAAAT CGACAAAAAG 
    CAACCTTCAA AATTACAGCA GCAGCATCAG CAGCAGTATT GGAATAATAT GGATATGGGG 
    AGTGGTAAGT ATTATGCTGA GGCCAAGCAG AATCCTATGA ATCAAAAACA TACTCCCTTT 
    GAATCCAAAC AGCAACAAAA ACAACAATTC CAAACACTAA ATAACCTATA TTTTGCTCAG 
    TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

  • PubMed

    The nucleotide sequence of Saccharomyces cerevisiae chromosome VII.
    Nature387(6632 Suppl)81-4(1997 May)
    Tettelin H,Agostoni Carbone ML,Albermann K,Albers M,Arroyo J,Backes U,Barreiros T,Bertani I,Bjourson AJ,Br?ckner M,Bruschi CV,Carignani G,Castagnoli L,Cerdan E,Clemente ML,Coblenz A,Coglievina M,Coissac E,Defoor E,Del Bino S,Delius H,Delneri D,de Wergifosse P,Dujon B,Kleine K


    Life with 6000 genes.
    Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)
    Goffeau A,Barrell BG,Bussey H,Davis RW,Dujon B,Feldmann H,Galibert F,Hoheisel JD,Jacq C,Johnston M,Louis EJ,Mewes HW,Murakami Y,Philippsen P,Tettelin H,Oliver SG


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