VID30 cDNA ORF clone, Saccharomyces cerevisiae S288C

Gene
Clones
gRNAs

Summary

Gene Symbol	VID30
Entrez Gene ID	852624
Full Name	glucose-induced degradation complex subunit VID30
Synonyms	GID1
Gene Type	protein-coding
Organism	Saccharomyces cerevisiae S288C

mRNA and Protein(s)

mRNA	Protein	Name
NM_001181093.1	NP_011287.1	glucose-induced degradation complex subunit VID30

Pathways from BioSystems

Homologies

Kluyveromyces lactis	KLLA0A01221g	XP_451053.1
Eremothecium gossypii	AGOS_AFR242C	NP_985789.1
Saccharomyces cerevisiae (baker's yeast)	VID30	NP_011287.1

Gene Ontology
Bibliography

Related articles in PubMed

The nucleotide sequence of Saccharomyces cerevisiae chromosome VII.
Tettelin H, Agostoni Carbone ML, Albermann K, Albers M, Arroyo J, Backes U, Barreiros T, Bertani I, Bjourson AJ, Br?ckner M, Bruschi CV, Carignani G, Castagnoli L, Cerdan E, Clemente ML, Coblenz A, Coglievina M, Coissac E, Defoor E, Del Bino S, Delius H, Delneri D, de Wergifosse P, Dujon B, Kleine K
Nature387(6632 Suppl)81-4(1997 May)

Life with 6000 genes.
Goffeau A, Barrell BG, Bussey H, Davis RW, Dujon B, Feldmann H, Galibert F, Hoheisel JD, Jacq C, Johnston M, Louis EJ, Mewes HW, Murakami Y, Philippsen P, Tettelin H, Oliver SG
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

turnover of both Hxt3 and Hxt7 is dependent on the essential E3 ubiquitin ligase, Rsp5, indicating that the role of the Vid30c might be indirect of Hxt ubiquitylation
Title: Regulation of Hxt3 and Hxt7 turnover converges on the Vid30 complex and requires inactivation of the Ras/cAMP/PKA pathway in Saccharomyces cerevisiae.

Three members of the VID and GID gene families, VID30/GID1, GID2, and VID28/GID5 are needed for the rapamycin or nitrogen starvation-induced degradation of the high-affinity hexose transporter Hxt7p is shown here.
Title: Components of the Vid30c are needed for the rapamycin-induced degradation of the high-affinity hexose transporter Hxt7p in Saccharomyces cerevisiae.

The following VID30 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the VID30 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OSi02013	NM_001181093.1 Latest version!	Saccharomyces cerevisiae S288C glucose-induced degradation complex subunit VID30 (VID30), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$510.30 $729.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

VID30 ( NM_001181093.1 ) cDNA ORF clone, Saccharomyces cerevisiae S288C -> NP_011287.1 Saccharomyces cerevisiae S288C glucose-induced degradation complex subunit VID30 (VID30), partial mRNA.

Price & Availability↑

CloneID	OSi02013
Clone ID Related Accession (Same CDS sequence)	NM_001181093.1
Accession Version	NM_001181093.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2877bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-31
Organism	Saccharomyces cerevisiae S288C
Product	glucose-induced degradation complex subunit VID30
Comment	Comment: REVIEWED REFSEQ: This record has been curated by SGD. This record is derived from an annotated genomic sequence (NC_001139). ##Genome-Annotation-Data-START## Annotation Provider :: SGD Annotation Status :: Full Annotation Annotation Version :: R64-2-1 URL :: http://www.yeastgenome.org/ ##Genome-Annotation-Data-END## COMPLETENESS: incomplete on both ends.

ATGTCTGAAT ATATGGATGA CGTAGACCGC GAATTCATTA ACTGTTTGTT CCCGAGCTAC 
TTGCTGCAAC AGCCTGTGGC TTATGATTTA TGGATTTTAT ATTTGCAACA TAGAAAACTC 
TTTCACAAAC TGAAGAACAC TAACTTAATC AACGCTGATG AAAATCCCAC TGGCGTTGGA 
ATGGGCAGGA CAAAGTTAAC TGCTTTAACA AGGAAAGAAA TCTGGTCTAA ATTGATGAAT 
TTGGGTGTTC TAGGCACAAT TTCGTTTGAA GCTGTCAACG ACGATTACCT GATTCAAGTC 
TATAAATATT TCTATCCAGA CGTTAATGAT TTTACTTTGA GATTTGGTGT TAAAGATTCA 
AATAAAAATT CAGTGCGAGT TATGAAAGCT TCCTCAGACA TGAGGAAAAA TGCTCAGGAA 
CTATTAGAAC CCGTTTTATC CGAACGTGAA ATGGCGCTTA ACTCTAATAC CAGCCTGGAA 
AATGACAGAA ATGACGATGA TGATGACGAT GATGATGATG ACGATGATGA CGATGACGAT 
GACGACGACG ACGATGAATC CGATCTGGAA AGCTTAGAAG GGGAAGTGGA TACTGACACG 
GACGATAACA ACGAAGGAGA TGGCTCTGAC AATCACGAGG AAGGTGGAGA AGAAGGAAGT 
AGAGGAGCTG ATGCTGATGT GTCCAGTGCA CAGCAGCGTG CCGAAAGGGT TGCGGACCCA 
TGGATATATC AAAGATCTAG ATCAGCTATT AATATAGAAA CAGAATCAAG GAATTTGTGG 
GATACATCTG ACAAGAACAG TGGTCTACAA TACTACCCCC CTGATCAGTC TCCAAGTTCT 
TCCTTTTCTT CTCCTAGAGT ATCGTCTGGT AACGACAAAA ATGACAATGA GGCAACAAAT 
GTTTTGTCCA ACAGTGGTAG CAAAAAGAAA AACTCAATGA TCCCTGATAT TTACAAAATA 
CTTGGGTACT TTTTACCTTC TAGATGGCAA GCTCAACCAA ATAACAGCTT GCAGCTATCT 
CAAGATGGTA TTACTCATTT ACAACCTAAT CCTGATTATC ACTCATACAT GACTTATGAA 
AGATCAAGCG CTTCTTCTGC CTCTACCAGA AATAGACTGA GAACCTCTTT TGAGAACTCT 
GGGAAAGTGG ACTTTGCAGT CACTTGGGCA AATAAGTCAT TGCCAGACAA TAAGTTAACT 
ATATTCTATT ACGAGATTAA AGTTCTCAGT GTAACGAGTA CAGAGAGTGC TGAAAATAGT 
AACATTGTTA TCGGATACAA GTTAGTAGAG AATGAATTAA TGGAAGCGAC TACAAAAAAG 
AGTGTTTCTC GTTCAAGCGT GGCCGGTTCT AGTAGTAGCT TAGGTGGTAG TAATAACATG 
AGTTCCAATA GAGTACCTTC TACTTCTTTC ACTATGGAAG GTACTCAAAG GCGTGATTAT 
ATTTATGAAG GGGGCGTTTC AGCCATGTCT CTTAACGTGG ATGGTTCCAT AAATAAGTGT 
CAGAAATACG GATTTGATCT TAATGTATTT GGCTATTGCG GATTTGACGG TTTGATAACT 
AATTCTACAG AACAATCTAA GGAATATGCT AAGCCTTTTG GTAGGGATGA CGTTATTGGT 
TGTGGCATAA ATTTTATCGA TGGCTCAATT TTTTTCACGA AGAATGGTAT CCATTTAGGT 
AACGCATTCA CCGACTTGAA TGATCTAGAA TTCGTCCCAT ATGTGGCCTT ACGACCTGGG 
AATTCTATCA AAACAAATTT TGGGTTAAAT GAGGATTTTG TATTTGACAT TATTGGATAT 
CAAGATAAAT GGAAGAGTTT GGCGTATGAG CATATTTGTC GTGGACGCCA AATGGACGTT 
TCAATTGAAG AATTCGACTC TGATGAAAGT GAGGAAGATG AAACTGAAAA TGGGCCTGAA 
GAAAATAAAT CAACAAACGT TAATGAAGAT TTGATGGATA TTGATCAAGA GGATGGTGCC 
GCTGGTAATA AGGATACAAA GAAGCTTAAC GATGAGAAGG ACAACAATCT GAAGTTTCTT 
TTGGGAGAAG ATAATAGGTT TATAGATGGA AAGCTAGTTA GACCAGACGT GAACAATATT 
AACAACTTAA GCGTGGATGA CGGTTCATTG CCTAATACAT TAAACGTGAT GATAAATGAT 
TATTTGATCC ACGAAGGATT GGTGGATGTT GCAAAGGGGT TTTTGAAAGA TTTGCAAAAA 
GATGCGGTTA ATGTCAATGG GCAACATTCT GAAAGTAAAG ATGTTATCAG GCACAATGAA 
AGACAGATCA TGAAAGAAGA ACGTATGGTA AAGATAAGGC AAGAATTAAG ATACTTGATA 
AACAAAGGTC AAATTAGCAA ATGCATCAAT TATATCGATA ACGAAATTCC AGATCTGTTA 
AAAAATAATC TAGAATTGGT ATTTGAGTTA AAACTAGCGA ATTATCTGGT GATGATTAAA 
AAGAGTTCAT CCAAAGATGA TGACGAAATT GAAAATTTAA TTTTGAAGGG TCAAGAATTA 
TCTAATGAAT TTATCTACGA TACTAAAATT CCACAATCTT TGAGGGATAG GTTTAGTGGA 
CAATTGAGTA ATGTCTCTGC ATTGTTAGCA TATTCCAATC CACTTGTGGA GGCACCAAAG 
GAGATATCAG GATATTTGAG TGACGAATAC TTACAAGAGA GATTATTTCA AGTTAGTAAC 
AACACAATAC TAACATTTTT ACATAAGGAT AGCGAATGCG CATTAGAGAA TGTAATATCA 
AACACTAGAG CTATGCTTTC TACATTACTT GAGTATAACG CGTTCGGTTC AACCAACTCT 
TCAGATCCAA GATATTACAA AGCTATTAAC TTCGACGAAG ATGTGTTGAA TTTATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_011287.1
CDS	1..2877
Translation	MSEYMDDVDREFINCLFPSYLLQQPVAYDLWILYLQHRKLFHKLKNTNLINADENPTGVGMGRTKLTALTRKEIWSKLMNLGVLGTISFEAVNDDYLIQVYKYFYPDVNDFTLRFGVKDSNKNSVRVMKASSDMRKNAQELLEPVLSEREMALNSNTSLENDRNDDDDDDDDDDDDDDDDDDDDDESDLESLEGEVDTDTDDNNEGDGSDNHEEGGEEGSRGADADVSSAQQRAERVADPWIYQRSRSAINIETESRNLWDTSDKNSGLQYYPPDQSPSSSFSSPRVSSGNDKNDNEATNVLSNSGSKKKNSMIPDIYKILGYFLPSRWQAQPNNSLQLSQDGITHLQPNPDYHSYMTYERSSASSASTRNRLRTSFENSGKVDFAVTWANKSLPDNKLTIFYYEIKVLSVTSTESAENSNIVIGYKLVENELMEATTKKSVSRSSVAGSSSSLGGSNNMSSNRVPSTSFTMEGTQRRDYIYEGGVSAMSLNVDGSINKCQKYGFDLNVFGYCGFDGLITNSTEQSKEYAKPFGRDDVIGCGINFIDGSIFFTKNGIHLGNAFTDLNDLEFVPYVALRPGNSIKTNFGLNEDFVFDIIGYQDKWKSLAYEHICRGRQMDVSIEEFDSDESEEDETENGPEENKSTNVNEDLMDIDQEDGAAGNKDTKKLNDEKDNNLKFLLGEDNRFIDGKLVRPDVNNINNLSVDDGSLPNTLNVMINDYLIHEGLVDVAKGFLKDLQKDAVNVNGQHSESKDVIRHNERQIMKEERMVKIRQELRYLINKGQISKCINYIDNEIPDLLKNNLELVFELKLANYLVMIKKSSSKDDDEIENLILKGQELSNEFIYDTKIPQSLRDRFSGQLSNVSALLAYSNPLVEAPKEISGYLSDEYLQERLFQVSNNTILTFLHKDSECALENVISNTRAMLSTLLEYNAFGSTNSSDPRYYKAINFDEDVLNL

Target ORF information:

RefSeq Version	NM_001181093.1
Organism	Saccharomyces cerevisiae S288C
Definition	Saccharomyces cerevisiae S288C glucose-induced degradation complex subunit VID30 (VID30), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001181093.1

ORF Insert Sequence:

ATGTCTGAAT ATATGGATGA CGTAGACCGC GAATTCATTA ACTGTTTGTT CCCGAGCTAC 
TTGCTGCAAC AGCCTGTGGC TTATGATTTA TGGATTTTAT ATTTGCAACA TAGAAAACTC 
TTTCACAAAC TGAAGAACAC TAACTTAATC AACGCTGATG AAAATCCCAC TGGCGTTGGA 
ATGGGCAGGA CAAAGTTAAC TGCTTTAACA AGGAAAGAAA TCTGGTCTAA ATTGATGAAT 
TTGGGTGTTC TAGGCACAAT TTCGTTTGAA GCTGTCAACG ACGATTACCT GATTCAAGTC 
TATAAATATT TCTATCCAGA CGTTAATGAT TTTACTTTGA GATTTGGTGT TAAAGATTCA 
AATAAAAATT CAGTGCGAGT TATGAAAGCT TCCTCAGACA TGAGGAAAAA TGCTCAGGAA 
CTATTAGAAC CCGTTTTATC CGAACGTGAA ATGGCGCTTA ACTCTAATAC CAGCCTGGAA 
AATGACAGAA ATGACGATGA TGATGACGAT GATGATGATG ACGATGATGA CGATGACGAT 
GACGACGACG ACGATGAATC CGATCTGGAA AGCTTAGAAG GGGAAGTGGA TACTGACACG 
GACGATAACA ACGAAGGAGA TGGCTCTGAC AATCACGAGG AAGGTGGAGA AGAAGGAAGT 
AGAGGAGCTG ATGCTGATGT GTCCAGTGCA CAGCAGCGTG CCGAAAGGGT TGCGGACCCA 
TGGATATATC AAAGATCTAG ATCAGCTATT AATATAGAAA CAGAATCAAG GAATTTGTGG 
GATACATCTG ACAAGAACAG TGGTCTACAA TACTACCCCC CTGATCAGTC TCCAAGTTCT 
TCCTTTTCTT CTCCTAGAGT ATCGTCTGGT AACGACAAAA ATGACAATGA GGCAACAAAT 
GTTTTGTCCA ACAGTGGTAG CAAAAAGAAA AACTCAATGA TCCCTGATAT TTACAAAATA 
CTTGGGTACT TTTTACCTTC TAGATGGCAA GCTCAACCAA ATAACAGCTT GCAGCTATCT 
CAAGATGGTA TTACTCATTT ACAACCTAAT CCTGATTATC ACTCATACAT GACTTATGAA 
AGATCAAGCG CTTCTTCTGC CTCTACCAGA AATAGACTGA GAACCTCTTT TGAGAACTCT 
GGGAAAGTGG ACTTTGCAGT CACTTGGGCA AATAAGTCAT TGCCAGACAA TAAGTTAACT 
ATATTCTATT ACGAGATTAA AGTTCTCAGT GTAACGAGTA CAGAGAGTGC TGAAAATAGT 
AACATTGTTA TCGGATACAA GTTAGTAGAG AATGAATTAA TGGAAGCGAC TACAAAAAAG 
AGTGTTTCTC GTTCAAGCGT GGCCGGTTCT AGTAGTAGCT TAGGTGGTAG TAATAACATG 
AGTTCCAATA GAGTACCTTC TACTTCTTTC ACTATGGAAG GTACTCAAAG GCGTGATTAT 
ATTTATGAAG GGGGCGTTTC AGCCATGTCT CTTAACGTGG ATGGTTCCAT AAATAAGTGT 
CAGAAATACG GATTTGATCT TAATGTATTT GGCTATTGCG GATTTGACGG TTTGATAACT 
AATTCTACAG AACAATCTAA GGAATATGCT AAGCCTTTTG GTAGGGATGA CGTTATTGGT 
TGTGGCATAA ATTTTATCGA TGGCTCAATT TTTTTCACGA AGAATGGTAT CCATTTAGGT 
AACGCATTCA CCGACTTGAA TGATCTAGAA TTCGTCCCAT ATGTGGCCTT ACGACCTGGG 
AATTCTATCA AAACAAATTT TGGGTTAAAT GAGGATTTTG TATTTGACAT TATTGGATAT 
CAAGATAAAT GGAAGAGTTT GGCGTATGAG CATATTTGTC GTGGACGCCA AATGGACGTT 
TCAATTGAAG AATTCGACTC TGATGAAAGT GAGGAAGATG AAACTGAAAA TGGGCCTGAA 
GAAAATAAAT CAACAAACGT TAATGAAGAT TTGATGGATA TTGATCAAGA GGATGGTGCC 
GCTGGTAATA AGGATACAAA GAAGCTTAAC GATGAGAAGG ACAACAATCT GAAGTTTCTT 
TTGGGAGAAG ATAATAGGTT TATAGATGGA AAGCTAGTTA GACCAGACGT GAACAATATT 
AACAACTTAA GCGTGGATGA CGGTTCATTG CCTAATACAT TAAACGTGAT GATAAATGAT 
TATTTGATCC ACGAAGGATT GGTGGATGTT GCAAAGGGGT TTTTGAAAGA TTTGCAAAAA 
GATGCGGTTA ATGTCAATGG GCAACATTCT GAAAGTAAAG ATGTTATCAG GCACAATGAA 
AGACAGATCA TGAAAGAAGA ACGTATGGTA AAGATAAGGC AAGAATTAAG ATACTTGATA 
AACAAAGGTC AAATTAGCAA ATGCATCAAT TATATCGATA ACGAAATTCC AGATCTGTTA 
AAAAATAATC TAGAATTGGT ATTTGAGTTA AAACTAGCGA ATTATCTGGT GATGATTAAA 
AAGAGTTCAT CCAAAGATGA TGACGAAATT GAAAATTTAA TTTTGAAGGG TCAAGAATTA 
TCTAATGAAT TTATCTACGA TACTAAAATT CCACAATCTT TGAGGGATAG GTTTAGTGGA 
CAATTGAGTA ATGTCTCTGC ATTGTTAGCA TATTCCAATC CACTTGTGGA GGCACCAAAG 
GAGATATCAG GATATTTGAG TGACGAATAC TTACAAGAGA GATTATTTCA AGTTAGTAAC 
AACACAATAC TAACATTTTT ACATAAGGAT AGCGAATGCG CATTAGAGAA TGTAATATCA 
AACACTAGAG CTATGCTTTC TACATTACTT GAGTATAACG CGTTCGGTTC AACCAACTCT 
TCAGATCCAA GATATTACAA AGCTATTAAC TTCGACGAAG ATGTGTTGAA TTTATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The nucleotide sequence of Saccharomyces cerevisiae chromosome VII.
Nature387(6632 Suppl)81-4(1997 May)
Tettelin H,Agostoni Carbone ML,Albermann K,Albers M,Arroyo J,Backes U,Barreiros T,Bertani I,Bjourson AJ,Br?ckner M,Bruschi CV,Carignani G,Castagnoli L,Cerdan E,Clemente ML,Coblenz A,Coglievina M,Coissac E,Defoor E,Del Bino S,Delius H,Delneri D,de Wergifosse P,Dujon B,Kleine K

Life with 6000 genes.
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)
Goffeau A,Barrell BG,Bussey H,Davis RW,Dujon B,Feldmann H,Galibert F,Hoheisel JD,Jacq C,Johnston M,Louis EJ,Mewes HW,Murakami Y,Philippsen P,Tettelin H,Oliver SG

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VID30 cDNA ORF clone, Saccharomyces cerevisiae S288C

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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