APE3 cDNA ORF clone, Saccharomyces cerevisiae S288C

Gene
Clones
gRNAs

Summary

Gene Symbol	APE3
Entrez Gene ID	852589
Full Name	Ape3p
Synonyms	APY1
Gene Type	protein-coding
Organism	Saccharomyces cerevisiae S288C

mRNA and Protein(s)

mRNA	Protein	Name
NM_001178634.1	NP_009845.2	aminopeptidase Y

Pathways from BioSystems

Homologies

Kluyveromyces lactis	KLLA0D06424g	XP_453347.1
Eremothecium gossypii	AGOS_ADR123W	NP_984219.1
Saccharomyces cerevisiae (baker's yeast)	APE3	NP_009845.2
Magnaporthe oryzae (rice blast fungus)	MGG_09692	XP_003717664.1

Gene Ontology
Bibliography

Related articles in PubMed

Life with 6000 genes.
Goffeau A, Barrell BG, Bussey H, Davis RW, Dujon B, Feldmann H, Galibert F, Hoheisel JD, Jacq C, Johnston M, Louis EJ, Mewes HW, Murakami Y, Philippsen P, Tettelin H, Oliver SG
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)

Complete DNA sequence of yeast chromosome II.
Feldmann H, Aigle M, Aljinovic G, Andr? B, Baclet MC, Barthe C, Baur A, B?cam AM, Biteau N, Boles E, Brandt T, Brendel M, Br?ckner M, Bussereau F, Christiansen C, Contreras R, Crouzet M, Cziepluch C, D?molis N, Delaveau T, Doignon F, Domdey H, D?sterhus S, Dubois E, Dujon B, El Bakkoury M, Entian KD, Feurmann M, Fiers W, Fobo GM, Fritz C, Gassenhuber H, Glandsdorff N, Goffeau A, Grivell LA, de Haan M, Hein C, Herbert CJ, Hollenberg CP, Holmstr?m K, Jacq C, Jacquet M, Jauniaux JC, Jonniaux JL, Kalles?e T, Kiesau P, Kirchrath L, K?tter P, Korol S, Liebl S, Logghe M, Lohan AJ, Louis EJ, Li ZY, Maat MJ, Mallet L, Mannhaupt G, Messenguy F, Miosga T, Molemans F, M?ller S, Nasr F, Obermaier B, Perea J, Pi?rard A, Piravandi E, Pohl FM, Pohl TM, Potier S, Proft M, Purnelle B, Ramezani Rad M, Rieger M, Rose M, Schaaff-Gerstenschl?ger I, Scherens B, Schwarzlose C, Skala J, Slonimski PP, Smits PH, Souciet JL, Steensma HY, Stucka R, Urrestarazu A, van der Aart QJ, van Dyck L, Vassarotti A, Vetter I, Vierendeels F, Vissers S, Wagner G, de Wergifosse P, Wolfe KH, Zagulski M, Zimmermann FK, Mewes HW, Kleine K
The EMBO journal13(24)5795-809(1994 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Therefore, S. cerevisiae was transformed to pYES2::APE3::GFP, accumulating a large amount of APE3, resulting in increased lysosomal activity. Increase in endogenous levels of lysosomes and their activity following genetic modification can lead to its use in applications such as antimicrobial agents and apoptosis-inducing materials for cancer cells
Title: Enhanced lysosomal activity by overexpressed aminopeptidase Y in Saccharomyces cerevisiae.

The following APE3 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the APE3 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OSi01981	NM_001178634.1 Latest version!	Saccharomyces cerevisiae S288C aminopeptidase Y (APE3), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

APE3 ( NM_001178634.1 ) cDNA ORF clone, Saccharomyces cerevisiae S288C -> NP_009845.2 Saccharomyces cerevisiae S288C aminopeptidase Y (APE3), partial mRNA.

Price & Availability↑

CloneID	OSi01981
Clone ID Related Accession (Same CDS sequence)	NM_001178634.1
Accession Version	NM_001178634.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1614bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-31
Organism	Saccharomyces cerevisiae S288C
Product	aminopeptidase Y
Comment	Comment: REVIEWED REFSEQ: This record has been curated by SGD. This record is derived from an annotated genomic sequence (NC_001134). ##Genome-Annotation-Data-START## Annotation Provider :: SGD Annotation Status :: Full Annotation Annotation Version :: R64-2-1 URL :: http://www.yeastgenome.org/ ##Genome-Annotation-Data-END## COMPLETENESS: incomplete on both ends.

ATGCACTTTT CTTTGAAGCA ATTGGCTGTC GCTGCGTTTT ACGCTACCAA TTTAGGCTCT 
GCCTATGTCA TTCCTCAATT CTTCCAGGAA GCCTTTCAAC AAGAAGAGCC AATTGAGAAT 
TATCTTCCGC AATTAAACGA CGATGATAGC TCTGCCGTTG CTGCCAACAT TCCGAAGCCA 
CATATTCCTT ACTTCATGAA ACCACATGTA GAAAGTGAAA AATTGCAAGA TAAAATCAAA 
GTTGACGATT TGAATGCTAC TGCTTGGGAC CTCTACCGTT TAGCTAACTA TTCTACTCCT 
GACTACGGTC ACCCTACCCG TGTGATCGGG TCCAAGGGTC ATAACAAGAC TATGGAATAC 
ATATTGAATG TTTTCGATGA CATGCAGGAC TACTATGATG TTTCTTTGCA AGAGTTCGAA 
GCTTTATCCG GTAAGATCAT CTCTTTCAAC CTCTCTGACG CCGAAACCGG TAAAAGCTTT 
GCTAACACTA CAGCTTTCGC ACTTTCTCCA CCTGTGGACG GGTTCGTAGG TAAGCTAGTC 
GAAATTCCTA ACTTGGGATG TGAAGAGAAG GATTACGCCT CTGTAGTCCC ACCAAGACAC 
AACGAAAAGC AAATTGCCCT TATCGAAAGA GGTAAGTGTC CTTTTGGCGA TAAGAGTAAT 
TTGGCCGGTA AGTTCGGTTT CACCGCTGTG GTCATTTACG ACAACGAACC TAAATCTAAA 
GAAGGACTAC ACGGTACATT AGGCGAACCT ACCAAGCACA CTGTCGCCAC CGTCGGTGTT 
CCATATAAAG TTGGTAAAAA ATTGATTGCC AATATTGCAT TGAACATCGA TTATTCATTA 
TATTTTGCCA TGGACTCTTA TGTAGAATTT ATCAAGACCC AGAACATCAT TGCCGACACA 
AAACACGGTG ATCCTGATAA TATTGTTGCC CTTGGTGCTC ATTCAGACTC TGTTGAGGAG 
GGCCCAGGTA TCAACGACGA TGGTTCCGGT ACTATTTCCC TATTGAACGT CGCTAAGCAA 
TTGACCCATT TCAAGATCAA TAACAAGGTC CGTTTTGCAT GGTGGGCTGC TGAAGAAGAA 
GGTTTGCTAG GCTCTAACTT CTACGCTTAT AACTTGACCA AAGAAGAAAA CTCCAAGATC 
AGAGTATTTA TGGACTATGA CATGATGGCT TCTCCAAACT ATGAATACGA AATTTATGAT 
GCGAACAACA AGGAGAACCC TAAGGGGTCT GAAGAGTTGA AAAACCTGTA CGTAGACTAC 
TACAAGGCTC ATCACTTGAA CTACACTTTG GTCCCATTTG ACGGTAGATC CGATTATGTC 
GGGTTTATCA ACAACGGAAT TCCAGCCGGT GGTATTGCCA CTGGTGCCGA AAAGAATAAC 
GTCAACAACG GAAAGGTCTT AGACAGATGC TACCATCAAT TATGTGATGA TGTCTCTAAC 
TTATCCTGGG ACGCATTCAT TACCAACACC AAGTTGATTG CTCACTCTGT GGCCACCTAT 
GCTGACTCTT TCGAGGGTTT CCCAAAAAGA GAAACCCAAA AGCACAAAGA GGTGGACATA 
TTGAATGCTC AACAACCACA ATTTAAGTAC AGGGCCGACT TCTTGATTAT TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_009845.2
CDS	1..1614
Translation	MHFSLKQLAVAAFYATNLGSAYVIPQFFQEAFQQEEPIENYLPQLNDDDSSAVAANIPKPHIPYFMKPHVESEKLQDKIKVDDLNATAWDLYRLANYSTPDYGHPTRVIGSKGHNKTMEYILNVFDDMQDYYDVSLQEFEALSGKIISFNLSDAETGKSFANTTAFALSPPVDGFVGKLVEIPNLGCEEKDYASVVPPRHNEKQIALIERGKCPFGDKSNLAGKFGFTAVVIYDNEPKSKEGLHGTLGEPTKHTVATVGVPYKVGKKLIANIALNIDYSLYFAMDSYVEFIKTQNIIADTKHGDPDNIVALGAHSDSVEEGPGINDDGSGTISLLNVAKQLTHFKINNKVRFAWWAAEEEGLLGSNFYAYNLTKEENSKIRVFMDYDMMASPNYEYEIYDANNKENPKGSEELKNLYVDYYKAHHLNYTLVPFDGRSDYVGFINNGIPAGGIATGAEKNNVNNGKVLDRCYHQLCDDVSNLSWDAFITNTKLIAHSVATYADSFEGFPKRETQKHKEVDILNAQQPQFKYRADFLII

Target ORF information:

RefSeq Version	NM_001178634.1
Organism	Saccharomyces cerevisiae S288C
Definition	Saccharomyces cerevisiae S288C aminopeptidase Y (APE3), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001178634.1

ORF Insert Sequence:

ATGCACTTTT CTTTGAAGCA ATTGGCTGTC GCTGCGTTTT ACGCTACCAA TTTAGGCTCT 
GCCTATGTCA TTCCTCAATT CTTCCAGGAA GCCTTTCAAC AAGAAGAGCC AATTGAGAAT 
TATCTTCCGC AATTAAACGA CGATGATAGC TCTGCCGTTG CTGCCAACAT TCCGAAGCCA 
CATATTCCTT ACTTCATGAA ACCACATGTA GAAAGTGAAA AATTGCAAGA TAAAATCAAA 
GTTGACGATT TGAATGCTAC TGCTTGGGAC CTCTACCGTT TAGCTAACTA TTCTACTCCT 
GACTACGGTC ACCCTACCCG TGTGATCGGG TCCAAGGGTC ATAACAAGAC TATGGAATAC 
ATATTGAATG TTTTCGATGA CATGCAGGAC TACTATGATG TTTCTTTGCA AGAGTTCGAA 
GCTTTATCCG GTAAGATCAT CTCTTTCAAC CTCTCTGACG CCGAAACCGG TAAAAGCTTT 
GCTAACACTA CAGCTTTCGC ACTTTCTCCA CCTGTGGACG GGTTCGTAGG TAAGCTAGTC 
GAAATTCCTA ACTTGGGATG TGAAGAGAAG GATTACGCCT CTGTAGTCCC ACCAAGACAC 
AACGAAAAGC AAATTGCCCT TATCGAAAGA GGTAAGTGTC CTTTTGGCGA TAAGAGTAAT 
TTGGCCGGTA AGTTCGGTTT CACCGCTGTG GTCATTTACG ACAACGAACC TAAATCTAAA 
GAAGGACTAC ACGGTACATT AGGCGAACCT ACCAAGCACA CTGTCGCCAC CGTCGGTGTT 
CCATATAAAG TTGGTAAAAA ATTGATTGCC AATATTGCAT TGAACATCGA TTATTCATTA 
TATTTTGCCA TGGACTCTTA TGTAGAATTT ATCAAGACCC AGAACATCAT TGCCGACACA 
AAACACGGTG ATCCTGATAA TATTGTTGCC CTTGGTGCTC ATTCAGACTC TGTTGAGGAG 
GGCCCAGGTA TCAACGACGA TGGTTCCGGT ACTATTTCCC TATTGAACGT CGCTAAGCAA 
TTGACCCATT TCAAGATCAA TAACAAGGTC CGTTTTGCAT GGTGGGCTGC TGAAGAAGAA 
GGTTTGCTAG GCTCTAACTT CTACGCTTAT AACTTGACCA AAGAAGAAAA CTCCAAGATC 
AGAGTATTTA TGGACTATGA CATGATGGCT TCTCCAAACT ATGAATACGA AATTTATGAT 
GCGAACAACA AGGAGAACCC TAAGGGGTCT GAAGAGTTGA AAAACCTGTA CGTAGACTAC 
TACAAGGCTC ATCACTTGAA CTACACTTTG GTCCCATTTG ACGGTAGATC CGATTATGTC 
GGGTTTATCA ACAACGGAAT TCCAGCCGGT GGTATTGCCA CTGGTGCCGA AAAGAATAAC 
GTCAACAACG GAAAGGTCTT AGACAGATGC TACCATCAAT TATGTGATGA TGTCTCTAAC 
TTATCCTGGG ACGCATTCAT TACCAACACC AAGTTGATTG CTCACTCTGT GGCCACCTAT 
GCTGACTCTT TCGAGGGTTT CCCAAAAAGA GAAACCCAAA AGCACAAAGA GGTGGACATA 
TTGAATGCTC AACAACCACA ATTTAAGTAC AGGGCCGACT TCTTGATTAT TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Life with 6000 genes.
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)
Goffeau A,Barrell BG,Bussey H,Davis RW,Dujon B,Feldmann H,Galibert F,Hoheisel JD,Jacq C,Johnston M,Louis EJ,Mewes HW,Murakami Y,Philippsen P,Tettelin H,Oliver SG

Complete DNA sequence of yeast chromosome II.
The EMBO journal13(24)5795-809(1994 Dec)
Feldmann H,Aigle M,Aljinovic G,Andr? B,Baclet MC,Barthe C,Baur A,B?cam AM,Biteau N,Boles E,Brandt T,Brendel M,Br?ckner M,Bussereau F,Christiansen C,Contreras R,Crouzet M,Cziepluch C,D?molis N,Delaveau T,Doignon F,Domdey H,D?sterhus S,Dubois E,Dujon B,El Bakkoury M,Entian KD,Feurmann M,Fiers W,Fobo GM,Fritz C,Gassenhuber H,Glandsdorff N,Goffeau A,Grivell LA,de Haan M,Hein C,Herbert CJ,Hollenberg CP,Holmstr?m K,Jacq C,Jacquet M,Jauniaux JC,Jonniaux JL,Kalles?e T,Kiesau P,Kirchrath L,K?tter P,Korol S,Liebl S,Logghe M,Lohan AJ,Louis EJ,Li ZY,Maat MJ,Mallet L,Mannhaupt G,Messenguy F,Miosga T,Molemans F,M?ller S,Nasr F,Obermaier B,Perea J,Pi?rard A,Piravandi E,Pohl FM,Pohl TM,Potier S,Proft M,Purnelle B,Ramezani Rad M,Rieger M,Rose M,Schaaff-Gerstenschl?ger I,Scherens B,Schwarzlose C,Skala J,Slonimski PP,Smits PH,Souciet JL,Steensma HY,Stucka R,Urrestarazu A,van der Aart QJ,van Dyck L,Vassarotti A,Vetter I,Vierendeels F,Vissers S,Wagner G,de Wergifosse P,Wolfe KH,Zagulski M,Zimmermann FK,Mewes HW,Kleine K

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APE3 cDNA ORF clone, Saccharomyces cerevisiae S288C

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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