HIS7 cDNA ORF clone, Saccharomyces cerevisiae S288C

Gene
Clones
gRNAs

Summary

Gene Symbol	HIS7
Entrez Gene ID	852550
Full Name	imidazoleglycerol-phosphate synthase
Gene Type	protein-coding
Organism	Saccharomyces cerevisiae S288C

mRNA and Protein(s)

mRNA	Protein	Name
NM_001178596.3	NP_009807.3	imidazoleglycerol-phosphate synthase

Pathways from BioSystems

Homologies

Eremothecium gossypii	AGOS_AFL046W	NP_985502.1
Magnaporthe oryzae (rice blast fungus)	MGG_00253	XP_003718853.1
Kluyveromyces lactis	KLLA0F06578g	XP_455378.1
Neurospora crassa	NCU07156	XP_957381.1
Schizosaccharomyces pombe (fission yeast)	his4	NP_596494.2
Arabidopsis thaliana (thale cress)	AT-HF	NP_194420.1
Oryza sativa (rice)	Os03g0256400	NP_001049598.1
Saccharomyces cerevisiae (baker's yeast)	HIS7	NP_009807.3

Gene Ontology
Bibliography

Related articles in PubMed

Life with 6000 genes.
Goffeau A, Barrell BG, Bussey H, Davis RW, Dujon B, Feldmann H, Galibert F, Hoheisel JD, Jacq C, Johnston M, Louis EJ, Mewes HW, Murakami Y, Philippsen P, Tettelin H, Oliver SG
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)

Complete DNA sequence of yeast chromosome II.
Feldmann H, Aigle M, Aljinovic G, Andr? B, Baclet MC, Barthe C, Baur A, B?cam AM, Biteau N, Boles E, Brandt T, Brendel M, Br?ckner M, Bussereau F, Christiansen C, Contreras R, Crouzet M, Cziepluch C, D?molis N, Delaveau T, Doignon F, Domdey H, D?sterhus S, Dubois E, Dujon B, El Bakkoury M, Entian KD, Feurmann M, Fiers W, Fobo GM, Fritz C, Gassenhuber H, Glandsdorff N, Goffeau A, Grivell LA, de Haan M, Hein C, Herbert CJ, Hollenberg CP, Holmstr?m K, Jacq C, Jacquet M, Jauniaux JC, Jonniaux JL, Kalles?e T, Kiesau P, Kirchrath L, K?tter P, Korol S, Liebl S, Logghe M, Lohan AJ, Louis EJ, Li ZY, Maat MJ, Mallet L, Mannhaupt G, Messenguy F, Miosga T, Molemans F, M?ller S, Nasr F, Obermaier B, Perea J, Pi?rard A, Piravandi E, Pohl FM, Pohl TM, Potier S, Proft M, Purnelle B, Ramezani Rad M, Rieger M, Rose M, Schaaff-Gerstenschl?ger I, Scherens B, Schwarzlose C, Skala J, Slonimski PP, Smits PH, Souciet JL, Steensma HY, Stucka R, Urrestarazu A, van der Aart QJ, van Dyck L, Vassarotti A, Vetter I, Vierendeels F, Vissers S, Wagner G, de Wergifosse P, Wolfe KH, Zagulski M, Zimmermann FK, Mewes HW, Kleine K
The EMBO journal13(24)5795-809(1994 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Imidazole glycerol phosphate synthase the glutaminase and N1-[(5'-phosphoribulosyl)-formimino]-5-aminoimidazole-4-carboxamide ribonucleotide binding sites are separated by 30 A
Title: Reaction coupling through interdomain contacts in imidazole glycerol phosphate synthase.

The following HIS7 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the HIS7 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OSi01944	NM_001178596.3 Latest version!	Saccharomyces cerevisiae S288C imidazoleglycerol-phosphate synthase (HIS7), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

HIS7 ( NM_001178596.3 ) cDNA ORF clone, Saccharomyces cerevisiae S288C -> NP_009807.3 Saccharomyces cerevisiae S288C imidazoleglycerol-phosphate synthase (HIS7), partial mRNA.

Price & Availability↑

CloneID	OSi01944
Clone ID Related Accession (Same CDS sequence)	NM_001178596.3
Accession Version	NM_001178596.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1659bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-31
Organism	Saccharomyces cerevisiae S288C
Product	imidazoleglycerol-phosphate synthase
Comment	Comment: REVIEWED REFSEQ: This record has been curated by SGD. This record is derived from an annotated genomic sequence (NC_001134). On Jul 30, 2012 this sequence version replaced NM_001178596.2. ##Genome-Annotation-Data-START## Annotation Provider :: SGD Annotation Status :: Full Annotation Annotation Version :: R64-2-1 URL :: http://www.yeastgenome.org/ ##Genome-Annotation-Data-END## COMPLETENESS: incomplete on both ends.

ATGCCGGTCG TTCACGTGAT TGACGTTGAA AGTGGTAACC TACAGTCACT AACCAATGCA 
ATTGAGCATT TAGGTTACGA AGTACAACTG GTGAAATCAC CAAAGGATTT TAACATATCA 
GGCACGTCAA GATTGATTTT GCCTGGTGTC GGAAATTATG GCCATTTCGT CGACAATTTA 
TTTAATAGAG GATTCGAAAA GCCGATAAGA GAATACATTG AATCTGGAAA ACCAATAATG 
GGAATTTGCG TCGGGCTACA AGCGCTCTTT GCCGGTTCCG TGGAAAGCCC TAAGAGTACG 
GGTCTGAACT ACATTGATTT TAAGTTGTCC AGGTTCGATG ATTCAGAAAA GCCAGTACCA 
GAAATAGGTT GGAATTCTTG CATTCCCTCG GAAAACCTAT TCTTTGGATT GGATCCATAC 
AAGAGGTACT ATTTCGTCCA TTCTTTTGCT GCCATTCTGA ATTCAGAAAA GAAAAAAAAC 
CTAGAAAATG ACGGTTGGAA AATTGCAAAA GCTAAGTACG GTTCAGAGGA ATTTATTGCG 
GCAGTCAACA AGAATAATAT ATTCGCTACT CAGTTCCATC CTGAAAAATC AGGTAAAGCT 
GGTTTGAACG TCATTGAGAA TTTTTTGAAG CAACAAAGTC CTCCGATTCC AAACTATAGT 
GCGGAAGAGA AGGAACTCTT AATGAATGAC TATTCAAATT ATGGTCTAAC ACGCAGAATT 
ATTGCTTGTC TTGATGTACG TACTAATGAC CAAGGTGATT TGGTGGTTAC TAAAGGTGAT 
CAATACGATG TACGTGAAAA AAGTGATGGT AAAGGTGTTA GAAACCTTGG TAAGCCTGTT 
CAGTTGGCAC AGAAATATTA CCAACAGGGT GCGGATGAAG TAACATTTTT GAATATAACT 
TCTTTTAGAG ATTGTCCTTT GAAGGATACT CCGATGCTAG AGGTTCTGAA ACAAGCCGCA 
AAGACAGTCT TTGTTCCATT GACAGTCGGT GGGGGGATCA AGGATATTGT CGATGTTGAT 
GGAACCAAAA TACCTGCTTT AGAAGTTGCA AGTCTATACT TCAGATCTGG TGCTGATAAA 
GTATCGATCG GTACGGATGC AGTCTATGCA GCCGAAAAAT ACTACGAGTT GGGTAACAGA 
GGAGATGGAA CGTCACCAAT AGAGACAATC TCGAAAGCAT ACGGTGCTCA GGCAGTTGTT 
ATTTCTGTCG ACCCTAAGAG AGTATATGTA AATTCACAAG CAGATACGAA GAACAAAGTC 
TTCGAGACAG AATATCCGGG CCCCAATGGA GAGAAATACT GCTGGTACCA ATGTACAATC 
AAAGGTGGAA GAGAATCTAG AGACCTTGGT GTGTGGGAAT TAACAAGGGC ATGTGAAGCT 
CTAGGTGCTG GGGAGATTTT ATTGAACTGC ATAGACAAGG ATGGCTCTAA TTCTGGTTAT 
GATCTGGAAT TGATAGAACA TGTTAAAGAT GCGGTCAAGA TTCCCGTCAT TGCATCCAGT 
GGCGCCGGTG TACCCGAACA TTTCGAAGAG GCCTTCCTAA AGACCCGCGC AGATGCTTGC 
TTGGGTGCAG GTATGTTCCA CAGAGGTGAA TTCACTGTTA ACGATGTAAA GGAGTATTTA 
CTAGAGCACG GATTAAAGGT TAGAATGGAT GAAGAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_009807.3
CDS	1..1659
Translation	MPVVHVIDVESGNLQSLTNAIEHLGYEVQLVKSPKDFNISGTSRLILPGVGNYGHFVDNLFNRGFEKPIREYIESGKPIMGICVGLQALFAGSVESPKSTGLNYIDFKLSRFDDSEKPVPEIGWNSCIPSENLFFGLDPYKRYYFVHSFAAILNSEKKKNLENDGWKIAKAKYGSEEFIAAVNKNNIFATQFHPEKSGKAGLNVIENFLKQQSPPIPNYSAEEKELLMNDYSNYGLTRRIIACLDVRTNDQGDLVVTKGDQYDVREKSDGKGVRNLGKPVQLAQKYYQQGADEVTFLNITSFRDCPLKDTPMLEVLKQAAKTVFVPLTVGGGIKDIVDVDGTKIPALEVASLYFRSGADKVSIGTDAVYAAEKYYELGNRGDGTSPIETISKAYGAQAVVISVDPKRVYVNSQADTKNKVFETEYPGPNGEKYCWYQCTIKGGRESRDLGVWELTRACEALGAGEILLNCIDKDGSNSGYDLELIEHVKDAVKIPVIASSGAGVPEHFEEAFLKTRADACLGAGMFHRGEFTVNDVKEYLLEHGLKVRMDEE

Target ORF information:

RefSeq Version	NM_001178596.3
Organism	Saccharomyces cerevisiae S288C
Definition	Saccharomyces cerevisiae S288C imidazoleglycerol-phosphate synthase (HIS7), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001178596.3

ORF Insert Sequence:

ATGCCGGTCG TTCACGTGAT TGACGTTGAA AGTGGTAACC TACAGTCACT AACCAATGCA 
ATTGAGCATT TAGGTTACGA AGTACAACTG GTGAAATCAC CAAAGGATTT TAACATATCA 
GGCACGTCAA GATTGATTTT GCCTGGTGTC GGAAATTATG GCCATTTCGT CGACAATTTA 
TTTAATAGAG GATTCGAAAA GCCGATAAGA GAATACATTG AATCTGGAAA ACCAATAATG 
GGAATTTGCG TCGGGCTACA AGCGCTCTTT GCCGGTTCCG TGGAAAGCCC TAAGAGTACG 
GGTCTGAACT ACATTGATTT TAAGTTGTCC AGGTTCGATG ATTCAGAAAA GCCAGTACCA 
GAAATAGGTT GGAATTCTTG CATTCCCTCG GAAAACCTAT TCTTTGGATT GGATCCATAC 
AAGAGGTACT ATTTCGTCCA TTCTTTTGCT GCCATTCTGA ATTCAGAAAA GAAAAAAAAC 
CTAGAAAATG ACGGTTGGAA AATTGCAAAA GCTAAGTACG GTTCAGAGGA ATTTATTGCG 
GCAGTCAACA AGAATAATAT ATTCGCTACT CAGTTCCATC CTGAAAAATC AGGTAAAGCT 
GGTTTGAACG TCATTGAGAA TTTTTTGAAG CAACAAAGTC CTCCGATTCC AAACTATAGT 
GCGGAAGAGA AGGAACTCTT AATGAATGAC TATTCAAATT ATGGTCTAAC ACGCAGAATT 
ATTGCTTGTC TTGATGTACG TACTAATGAC CAAGGTGATT TGGTGGTTAC TAAAGGTGAT 
CAATACGATG TACGTGAAAA AAGTGATGGT AAAGGTGTTA GAAACCTTGG TAAGCCTGTT 
CAGTTGGCAC AGAAATATTA CCAACAGGGT GCGGATGAAG TAACATTTTT GAATATAACT 
TCTTTTAGAG ATTGTCCTTT GAAGGATACT CCGATGCTAG AGGTTCTGAA ACAAGCCGCA 
AAGACAGTCT TTGTTCCATT GACAGTCGGT GGGGGGATCA AGGATATTGT CGATGTTGAT 
GGAACCAAAA TACCTGCTTT AGAAGTTGCA AGTCTATACT TCAGATCTGG TGCTGATAAA 
GTATCGATCG GTACGGATGC AGTCTATGCA GCCGAAAAAT ACTACGAGTT GGGTAACAGA 
GGAGATGGAA CGTCACCAAT AGAGACAATC TCGAAAGCAT ACGGTGCTCA GGCAGTTGTT 
ATTTCTGTCG ACCCTAAGAG AGTATATGTA AATTCACAAG CAGATACGAA GAACAAAGTC 
TTCGAGACAG AATATCCGGG CCCCAATGGA GAGAAATACT GCTGGTACCA ATGTACAATC 
AAAGGTGGAA GAGAATCTAG AGACCTTGGT GTGTGGGAAT TAACAAGGGC ATGTGAAGCT 
CTAGGTGCTG GGGAGATTTT ATTGAACTGC ATAGACAAGG ATGGCTCTAA TTCTGGTTAT 
GATCTGGAAT TGATAGAACA TGTTAAAGAT GCGGTCAAGA TTCCCGTCAT TGCATCCAGT 
GGCGCCGGTG TACCCGAACA TTTCGAAGAG GCCTTCCTAA AGACCCGCGC AGATGCTTGC 
TTGGGTGCAG GTATGTTCCA CAGAGGTGAA TTCACTGTTA ACGATGTAAA GGAGTATTTA 
CTAGAGCACG GATTAAAGGT TAGAATGGAT GAAGAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Life with 6000 genes.
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)
Goffeau A,Barrell BG,Bussey H,Davis RW,Dujon B,Feldmann H,Galibert F,Hoheisel JD,Jacq C,Johnston M,Louis EJ,Mewes HW,Murakami Y,Philippsen P,Tettelin H,Oliver SG

Complete DNA sequence of yeast chromosome II.
The EMBO journal13(24)5795-809(1994 Dec)
Feldmann H,Aigle M,Aljinovic G,Andr? B,Baclet MC,Barthe C,Baur A,B?cam AM,Biteau N,Boles E,Brandt T,Brendel M,Br?ckner M,Bussereau F,Christiansen C,Contreras R,Crouzet M,Cziepluch C,D?molis N,Delaveau T,Doignon F,Domdey H,D?sterhus S,Dubois E,Dujon B,El Bakkoury M,Entian KD,Feurmann M,Fiers W,Fobo GM,Fritz C,Gassenhuber H,Glandsdorff N,Goffeau A,Grivell LA,de Haan M,Hein C,Herbert CJ,Hollenberg CP,Holmstr?m K,Jacq C,Jacquet M,Jauniaux JC,Jonniaux JL,Kalles?e T,Kiesau P,Kirchrath L,K?tter P,Korol S,Liebl S,Logghe M,Lohan AJ,Louis EJ,Li ZY,Maat MJ,Mallet L,Mannhaupt G,Messenguy F,Miosga T,Molemans F,M?ller S,Nasr F,Obermaier B,Perea J,Pi?rard A,Piravandi E,Pohl FM,Pohl TM,Potier S,Proft M,Purnelle B,Ramezani Rad M,Rieger M,Rose M,Schaaff-Gerstenschl?ger I,Scherens B,Schwarzlose C,Skala J,Slonimski PP,Smits PH,Souciet JL,Steensma HY,Stucka R,Urrestarazu A,van der Aart QJ,van Dyck L,Vassarotti A,Vetter I,Vierendeels F,Vissers S,Wagner G,de Wergifosse P,Wolfe KH,Zagulski M,Zimmermann FK,Mewes HW,Kleine K

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HIS7 cDNA ORF clone, Saccharomyces cerevisiae S288C

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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