RAD16 cDNA ORF clone, Saccharomyces cerevisiae S288C

Gene
Clones
gRNAs

Summary

Gene Symbol	RAD16
Entrez Gene ID	852411
Full Name	DNA repair protein RAD16
Synonyms	PSO5
Gene Type	protein-coding
Organism	Saccharomyces cerevisiae S288C

mRNA and Protein(s)

mRNA	Protein	Name
NM_001178462.1	NP_009672.1	DNA repair protein RAD16

Pathways from BioSystems

Homologies

Oryza sativa (rice)	Os07g0511500	NP_001059763.1
Saccharomyces cerevisiae (baker's yeast)	RAD16	NP_009672.1
Eremothecium gossypii	AGOS_ADL345C	NP_983750.2
Neurospora crassa	NCU03650	XP_961377.1
Kluyveromyces lactis	KLLA0B09240g	XP_451940.2
Schizosaccharomyces pombe (fission yeast)	rhp16	NP_587701.1
Magnaporthe oryzae (rice blast fungus)	MGG_07014	XP_003709772.1
Arabidopsis thaliana (thale cress)	AT1G05120	NP_172004.1

Gene Ontology
Bibliography

Related articles in PubMed

Life with 6000 genes.
Goffeau A, Barrell BG, Bussey H, Davis RW, Dujon B, Feldmann H, Galibert F, Hoheisel JD, Jacq C, Johnston M, Louis EJ, Mewes HW, Murakami Y, Philippsen P, Tettelin H, Oliver SG
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)

Complete DNA sequence of yeast chromosome II.
Feldmann H, Aigle M, Aljinovic G, Andr? B, Baclet MC, Barthe C, Baur A, B?cam AM, Biteau N, Boles E, Brandt T, Brendel M, Br?ckner M, Bussereau F, Christiansen C, Contreras R, Crouzet M, Cziepluch C, D?molis N, Delaveau T, Doignon F, Domdey H, D?sterhus S, Dubois E, Dujon B, El Bakkoury M, Entian KD, Feurmann M, Fiers W, Fobo GM, Fritz C, Gassenhuber H, Glandsdorff N, Goffeau A, Grivell LA, de Haan M, Hein C, Herbert CJ, Hollenberg CP, Holmstr?m K, Jacq C, Jacquet M, Jauniaux JC, Jonniaux JL, Kalles?e T, Kiesau P, Kirchrath L, K?tter P, Korol S, Liebl S, Logghe M, Lohan AJ, Louis EJ, Li ZY, Maat MJ, Mallet L, Mannhaupt G, Messenguy F, Miosga T, Molemans F, M?ller S, Nasr F, Obermaier B, Perea J, Pi?rard A, Piravandi E, Pohl FM, Pohl TM, Potier S, Proft M, Purnelle B, Ramezani Rad M, Rieger M, Rose M, Schaaff-Gerstenschl?ger I, Scherens B, Schwarzlose C, Skala J, Slonimski PP, Smits PH, Souciet JL, Steensma HY, Stucka R, Urrestarazu A, van der Aart QJ, van Dyck L, Vassarotti A, Vetter I, Vierendeels F, Vissers S, Wagner G, de Wergifosse P, Wolfe KH, Zagulski M, Zimmermann FK, Mewes HW, Kleine K
The EMBO journal13(24)5795-809(1994 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

use of rad7 Delta rad16 Delta mutants showed that Rad7-Rad16 is required for nucleotide excision repair of UV-lesions in three functionally distinct nucleosome-free regions, the promoter and 3'-end of the URA3 gene and the ARS1 origin of replication
Title: Functionally distinct nucleosome-free regions in yeast require Rad7 and Rad16 for nucleotide excision repair.

Rad16 mediates the ultraviolet-induced acetylation of histone H3, necessary for efficient global genome nucleotide-excision repair.
Title: Saccharomyces cerevisiae Rad16 mediates ultraviolet-dependent histone H3 acetylation required for efficient global genome nucleotide-excision repair.

Rad16 is solely responsible for repairing the nontranscribed strand of actively transcribed genes
Title: The roles of Rad16 and Rad26 in repairing repressed and actively transcribed genes in yeast.

The following RAD16 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the RAD16 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OSi01820	NM_001178462.1 Latest version!	Saccharomyces cerevisiae S288C DNA repair protein RAD16 (RAD16), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

RAD16 ( NM_001178462.1 ) cDNA ORF clone, Saccharomyces cerevisiae S288C -> NP_009672.1 Saccharomyces cerevisiae S288C DNA repair protein RAD16 (RAD16), partial mRNA.

Price & Availability↑

CloneID	OSi01820
Clone ID Related Accession (Same CDS sequence)	NM_001178462.1
Accession Version	NM_001178462.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2373bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-31
Organism	Saccharomyces cerevisiae S288C
Product	DNA repair protein RAD16
Comment	Comment: REVIEWED REFSEQ: This record has been curated by SGD. This record is derived from an annotated genomic sequence (NC_001134). ##Genome-Annotation-Data-START## Annotation Provider :: SGD Annotation Status :: Full Annotation Annotation Version :: R64-2-1 URL :: http://www.yeastgenome.org/ ##Genome-Annotation-Data-END## COMPLETENESS: incomplete on both ends.

ATGCAAGAAG GGGGCTTTAT CCGTAGAAGG CGTACGAGAA GTACGAAAAA AAGTGTTAAT 
TATAATGAAC TAAGCGATGA CGATACAGCT GTGAAAAACT CAAAAACTCT GCAGCTGAAA 
GGGAACAGCG AAAACGTAAA TGACAGCCAA GATGAAGAAT ACCGTGATGA TGCTACGCTC 
GTGAAATCAC CCGACGACGA TGACAAAGAT TTTATCATAG ACCTAACAGG TTCAGATAAA 
GAGCGGACCG CCACCGATGA GAATACTCAT GCGATCAAGA ATGATAATGA CGAGATAATA 
GAAATCAAGG AGGAACGTGA TGTTTCGGAT GACGACGAAC CGTTAACAAA GAAAAGGAAG 
ACAACTGCTA GGAAGAAGAA GAAAAAAACG AGCACCAAGA AAAAGTCACC GAAGGTAACC 
CCATATGAAA GAAACACTTT GCGATTATAT GAGCATCATC CTGAACTAAG GAATGTTTTC 
ACGGATTTGA AAAATGCACC TCCCTATGTC CCCCAAAGAT CCAAGCAGCC GGATGGTATG 
ACCATCAAAC TGCTACCTTT CCAGTTAGAA GGTCTTCATT GGCTAATATC TCAAGAGGAG 
AGCATTTATG CGGGCGGTGT TTTGGCAGAC GAAATGGGTA TGGGTAAGAC CATCCAAACT 
ATTGCCCTAT TAATGAACGA TTTGACTAAG TCTCCGTCTT TAGTTGTTGC CCCTACCGTG 
GCGCTGATGC AGTGGAAAAA CGAAATAGAA CAACATACAA AGGGACAACT GAAAATATAC 
ATTTATCACG GTGCTTCCAG AACCACGGAT ATCAAAGATT TGCAAGGCTA CGATGTTGTA 
CTAACCACTT ACGCAGTGCT GGAATCGGTA TTCAGAAAGC AAAACTACGG GTTTAGAAGG 
AAAAATGGAC TTTTCAAGCA GCCTTCCGTA TTGCATAATA TTGACTTTTA TAGAGTTATT 
CTGGATGAGG CACACAATAT CAAGGATAGA CAAAGCAATA CTGCTAGGGC TGTAAACAAC 
TTAAAAACGC AAAAGCGATG GTGCCTGTCG GGTACTCCGC TGCAAAATAG AATTGGTGAG 
ATGTATTCTT TGATCAGATT CTTAAATATC AATCCTTTCA CAAAGTACTT TTGTACCAAG 
TGTGATTGCG CTTCGAAGGA CTGGAAATTT ACGGATCGCA TGCATTGTGA CCATTGTAGT 
CACGTCATTA TGCAACACAC GAATTTCTTC AACCATTTCA TGTTGAAGAA CATTCAGAAA 
TTTGGTGTGG AAGGTCCTGG TTTAGAGTCT TTTAATAACA TTCAGACATT ATTGAAAAAC 
ATCATGCTGC GAAGAACTAA AGTGGAAAGA GCGGATGACT TGGGTCTACC GCCCAGAATT 
GTTACCGTGA GGAGAGACTT CTTCAATGAA GAGGAAAAAG ATCTTTACAG AAGTTTATAC 
ACAGATTCTA AAAGGAAGTA TAATTCCTTT GTTGAGGAGG GTGTTGTTCT AAACAATTAT 
GCAAACATTT TCACCCTAAT CACAAGAATG AGGCAACTGG CAGATCATCC TGATTTAGTT 
TTGAAAAGAT TAAACAATTT TCCTGGCGAT GATATCGGCG TCGTGATCTG CCAATTATGT 
AACGATGAAG CTGAGGAGCC CATTGAATCT AAATGTCACC ATAAGTTCTG TCGTTTATGC 
ATCAAAGAAT ATGTGGAATC TTTCATGGAA AACAACAATA AACTTACTTG TCCTGTTTGT 
CATATCGGGC TAAGTATCGA TTTGTCTCAA CCTGCTTTGG AAGTGGACCT TGATTCCTTC 
AAAAAGCAAA GTATTGTTAG CCGTCTAAAC ATGAGTGGCA AGTGGCAATC ATCAACGAAA 
ATCGAAGCAC TTGTGGAAGA ACTATACAAA CTGAGAAGCA ACAAGAGAAC GATTAAATCC 
ATTGTGTTTT CCCAGTTTAC CAGTATGCTG GATCTGGTAG AGTGGAGATT GAAAAGAGCT 
GGATTTCAAA CAGTGAAGCT TCAGGGTAGT ATGTCACCGA CGCAAAGAGA TGAAACCATC 
AAGTATTTCA TGAACAACAT TCAATGCGAG GTTTTCTTGG TCAGTTTAAA GGCGGGCGGT 
GTTGCTTTGA ATCTTTGTGA AGCTTCGCAA GTATTCATTT TAGACCCATG GTGGAACCCT 
AGTGTTGAAT GGCAAAGTGG TGATAGAGTT CATAGAATTG GTCAGTATCG ACCTGTGAAG 
ATCACAAGGT TTTGCATTGA GGATAGTATA GAAGCAAGAA TCATTGAATT ACAGGAAAAA 
AAGGCAAATA TGATTCATGC TACAATAAAC CAAGATGAAG CTGCCATTAG CAGACTAACG 
CCAGCTGATT TACAGTTCTT ATTCAATAAC TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_009672.1
CDS	1..2373
Translation	MQEGGFIRRRRTRSTKKSVNYNELSDDDTAVKNSKTLQLKGNSENVNDSQDEEYRDDATLVKSPDDDDKDFIIDLTGSDKERTATDENTHAIKNDNDEIIEIKEERDVSDDDEPLTKKRKTTARKKKKKTSTKKKSPKVTPYERNTLRLYEHHPELRNVFTDLKNAPPYVPQRSKQPDGMTIKLLPFQLEGLHWLISQEESIYAGGVLADEMGMGKTIQTIALLMNDLTKSPSLVVAPTVALMQWKNEIEQHTKGQLKIYIYHGASRTTDIKDLQGYDVVLTTYAVLESVFRKQNYGFRRKNGLFKQPSVLHNIDFYRVILDEAHNIKDRQSNTARAVNNLKTQKRWCLSGTPLQNRIGEMYSLIRFLNINPFTKYFCTKCDCASKDWKFTDRMHCDHCSHVIMQHTNFFNHFMLKNIQKFGVEGPGLESFNNIQTLLKNIMLRRTKVERADDLGLPPRIVTVRRDFFNEEEKDLYRSLYTDSKRKYNSFVEEGVVLNNYANIFTLITRMRQLADHPDLVLKRLNNFPGDDIGVVICQLCNDEAEEPIESKCHHKFCRLCIKEYVESFMENNNKLTCPVCHIGLSIDLSQPALEVDLDSFKKQSIVSRLNMSGKWQSSTKIEALVEELYKLRSNKRTIKSIVFSQFTSMLDLVEWRLKRAGFQTVKLQGSMSPTQRDETIKYFMNNIQCEVFLVSLKAGGVALNLCEASQVFILDPWWNPSVEWQSGDRVHRIGQYRPVKITRFCIEDSIEARIIELQEKKANMIHATINQDEAAISRLTPADLQFLFNN

Target ORF information:

RefSeq Version	NM_001178462.1
Organism	Saccharomyces cerevisiae S288C
Definition	Saccharomyces cerevisiae S288C DNA repair protein RAD16 (RAD16), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001178462.1

ORF Insert Sequence:

ATGCAAGAAG GGGGCTTTAT CCGTAGAAGG CGTACGAGAA GTACGAAAAA AAGTGTTAAT 
TATAATGAAC TAAGCGATGA CGATACAGCT GTGAAAAACT CAAAAACTCT GCAGCTGAAA 
GGGAACAGCG AAAACGTAAA TGACAGCCAA GATGAAGAAT ACCGTGATGA TGCTACGCTC 
GTGAAATCAC CCGACGACGA TGACAAAGAT TTTATCATAG ACCTAACAGG TTCAGATAAA 
GAGCGGACCG CCACCGATGA GAATACTCAT GCGATCAAGA ATGATAATGA CGAGATAATA 
GAAATCAAGG AGGAACGTGA TGTTTCGGAT GACGACGAAC CGTTAACAAA GAAAAGGAAG 
ACAACTGCTA GGAAGAAGAA GAAAAAAACG AGCACCAAGA AAAAGTCACC GAAGGTAACC 
CCATATGAAA GAAACACTTT GCGATTATAT GAGCATCATC CTGAACTAAG GAATGTTTTC 
ACGGATTTGA AAAATGCACC TCCCTATGTC CCCCAAAGAT CCAAGCAGCC GGATGGTATG 
ACCATCAAAC TGCTACCTTT CCAGTTAGAA GGTCTTCATT GGCTAATATC TCAAGAGGAG 
AGCATTTATG CGGGCGGTGT TTTGGCAGAC GAAATGGGTA TGGGTAAGAC CATCCAAACT 
ATTGCCCTAT TAATGAACGA TTTGACTAAG TCTCCGTCTT TAGTTGTTGC CCCTACCGTG 
GCGCTGATGC AGTGGAAAAA CGAAATAGAA CAACATACAA AGGGACAACT GAAAATATAC 
ATTTATCACG GTGCTTCCAG AACCACGGAT ATCAAAGATT TGCAAGGCTA CGATGTTGTA 
CTAACCACTT ACGCAGTGCT GGAATCGGTA TTCAGAAAGC AAAACTACGG GTTTAGAAGG 
AAAAATGGAC TTTTCAAGCA GCCTTCCGTA TTGCATAATA TTGACTTTTA TAGAGTTATT 
CTGGATGAGG CACACAATAT CAAGGATAGA CAAAGCAATA CTGCTAGGGC TGTAAACAAC 
TTAAAAACGC AAAAGCGATG GTGCCTGTCG GGTACTCCGC TGCAAAATAG AATTGGTGAG 
ATGTATTCTT TGATCAGATT CTTAAATATC AATCCTTTCA CAAAGTACTT TTGTACCAAG 
TGTGATTGCG CTTCGAAGGA CTGGAAATTT ACGGATCGCA TGCATTGTGA CCATTGTAGT 
CACGTCATTA TGCAACACAC GAATTTCTTC AACCATTTCA TGTTGAAGAA CATTCAGAAA 
TTTGGTGTGG AAGGTCCTGG TTTAGAGTCT TTTAATAACA TTCAGACATT ATTGAAAAAC 
ATCATGCTGC GAAGAACTAA AGTGGAAAGA GCGGATGACT TGGGTCTACC GCCCAGAATT 
GTTACCGTGA GGAGAGACTT CTTCAATGAA GAGGAAAAAG ATCTTTACAG AAGTTTATAC 
ACAGATTCTA AAAGGAAGTA TAATTCCTTT GTTGAGGAGG GTGTTGTTCT AAACAATTAT 
GCAAACATTT TCACCCTAAT CACAAGAATG AGGCAACTGG CAGATCATCC TGATTTAGTT 
TTGAAAAGAT TAAACAATTT TCCTGGCGAT GATATCGGCG TCGTGATCTG CCAATTATGT 
AACGATGAAG CTGAGGAGCC CATTGAATCT AAATGTCACC ATAAGTTCTG TCGTTTATGC 
ATCAAAGAAT ATGTGGAATC TTTCATGGAA AACAACAATA AACTTACTTG TCCTGTTTGT 
CATATCGGGC TAAGTATCGA TTTGTCTCAA CCTGCTTTGG AAGTGGACCT TGATTCCTTC 
AAAAAGCAAA GTATTGTTAG CCGTCTAAAC ATGAGTGGCA AGTGGCAATC ATCAACGAAA 
ATCGAAGCAC TTGTGGAAGA ACTATACAAA CTGAGAAGCA ACAAGAGAAC GATTAAATCC 
ATTGTGTTTT CCCAGTTTAC CAGTATGCTG GATCTGGTAG AGTGGAGATT GAAAAGAGCT 
GGATTTCAAA CAGTGAAGCT TCAGGGTAGT ATGTCACCGA CGCAAAGAGA TGAAACCATC 
AAGTATTTCA TGAACAACAT TCAATGCGAG GTTTTCTTGG TCAGTTTAAA GGCGGGCGGT 
GTTGCTTTGA ATCTTTGTGA AGCTTCGCAA GTATTCATTT TAGACCCATG GTGGAACCCT 
AGTGTTGAAT GGCAAAGTGG TGATAGAGTT CATAGAATTG GTCAGTATCG ACCTGTGAAG 
ATCACAAGGT TTTGCATTGA GGATAGTATA GAAGCAAGAA TCATTGAATT ACAGGAAAAA 
AAGGCAAATA TGATTCATGC TACAATAAAC CAAGATGAAG CTGCCATTAG CAGACTAACG 
CCAGCTGATT TACAGTTCTT ATTCAATAAC TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Life with 6000 genes.
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)
Goffeau A,Barrell BG,Bussey H,Davis RW,Dujon B,Feldmann H,Galibert F,Hoheisel JD,Jacq C,Johnston M,Louis EJ,Mewes HW,Murakami Y,Philippsen P,Tettelin H,Oliver SG

Complete DNA sequence of yeast chromosome II.
The EMBO journal13(24)5795-809(1994 Dec)
Feldmann H,Aigle M,Aljinovic G,Andr? B,Baclet MC,Barthe C,Baur A,B?cam AM,Biteau N,Boles E,Brandt T,Brendel M,Br?ckner M,Bussereau F,Christiansen C,Contreras R,Crouzet M,Cziepluch C,D?molis N,Delaveau T,Doignon F,Domdey H,D?sterhus S,Dubois E,Dujon B,El Bakkoury M,Entian KD,Feurmann M,Fiers W,Fobo GM,Fritz C,Gassenhuber H,Glandsdorff N,Goffeau A,Grivell LA,de Haan M,Hein C,Herbert CJ,Hollenberg CP,Holmstr?m K,Jacq C,Jacquet M,Jauniaux JC,Jonniaux JL,Kalles?e T,Kiesau P,Kirchrath L,K?tter P,Korol S,Liebl S,Logghe M,Lohan AJ,Louis EJ,Li ZY,Maat MJ,Mallet L,Mannhaupt G,Messenguy F,Miosga T,Molemans F,M?ller S,Nasr F,Obermaier B,Perea J,Pi?rard A,Piravandi E,Pohl FM,Pohl TM,Potier S,Proft M,Purnelle B,Ramezani Rad M,Rieger M,Rose M,Schaaff-Gerstenschl?ger I,Scherens B,Schwarzlose C,Skala J,Slonimski PP,Smits PH,Souciet JL,Steensma HY,Stucka R,Urrestarazu A,van der Aart QJ,van Dyck L,Vassarotti A,Vetter I,Vierendeels F,Vissers S,Wagner G,de Wergifosse P,Wolfe KH,Zagulski M,Zimmermann FK,Mewes HW,Kleine K

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RAD16 cDNA ORF clone, Saccharomyces cerevisiae S288C

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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