ENT5 cDNA ORF clone, Saccharomyces cerevisiae S288C

Gene
Clones
gRNAs

Summary

Gene Symbol	ENT5
Entrez Gene ID	851731
Full Name	Ent5p
Gene Type	protein-coding
Organism	Saccharomyces cerevisiae S288C

mRNA and Protein(s)

mRNA	Protein	Name
NM_001180460.3	NP_010437.3	Ent5p

Pathways from BioSystems

Homologies

Saccharomyces cerevisiae (baker's yeast)	ENT5	NP_010437.3
Kluyveromyces lactis	KLLA0D16654g	XP_453795.1
Eremothecium gossypii	AGOS_AGR046C	NP_986711.1

Gene Ontology
Bibliography

Related articles in PubMed

The nucleotide sequence of Saccharomyces cerevisiae chromosome IV.
Jacq C, Alt-M?rbe J, Andre B, Arnold W, Bahr A, Ballesta JP, Bargues M, Baron L, Becker A, Biteau N, Bl?cker H, Blugeon C, Boskovic J, Brandt P, Br?ckner M, Buitrago MJ, Coster F, Delaveau T, del Rey F, Dujon B, Eide LG, Garcia-Cantalejo JM, Goffeau A, Gomez-Peris A, Zaccaria P
Nature387(6632 Suppl)75-8(1997 May)

Life with 6000 genes.
Goffeau A, Barrell BG, Bussey H, Davis RW, Dujon B, Feldmann H, Galibert F, Hoheisel JD, Jacq C, Johnston M, Louis EJ, Mewes HW, Murakami Y, Philippsen P, Tettelin H, Oliver SG
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Above results facilitate the understanding of the ScEnt5-mediated vesicle coat formation process.
Title: Structural and functional insight into the N-terminal domain of the clathrin adaptor Ent5 from Saccharomyces cerevisiae.

Ent5 clathrin binding performs a mechanistic role in clathrin coat maturation, whereas Ent5 adaptor binding promotes cargo incorporation.
Title: Clathrin binding by the adaptor Ent5 promotes late stages of clathrin coat maturation.

Here we show not all acidic-phenylalanine motifs in Ent5 are active for yGGA2_GAE domain binding
Title: Structural basis for the specificity of the GAE domain of yGGA2 for its accessory proteins Ent3 and Ent5 .

the clathrin adaptors AP-1, Ent3p, Ent5p, and the Ggas cooperate in different ways to sort proteins between the TGN and the endosomes
Title: Ent3p and Ent5p exhibit cargo-specific functions in trafficking proteins between the trans-Golgi network and the endosomes in yeast.

These results differentiate Ent3p and Ent5p function in vivo, suggesting that Ent3p acts primarily with Gga proteins, whereas Ent5p acts with both AP-1 and Gga proteins but is more critical for AP-1-mediated transport.
Title: Distinct roles for TGN/endosome epsin-like adaptors Ent3p and Ent5p.

The following ENT5 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ENT5 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OSi01237	NM_001180460.3 Latest version!	Saccharomyces cerevisiae S288C Ent5p (ENT5), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

ENT5 ( NM_001180460.3 ) cDNA ORF clone, Saccharomyces cerevisiae S288C -> NP_010437.3 Saccharomyces cerevisiae S288C Ent5p (ENT5), partial mRNA.

Price & Availability↑

CloneID	OSi01237
Clone ID Related Accession (Same CDS sequence)	NM_001180460.3
Accession Version	NM_001180460.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1236bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-31
Organism	Saccharomyces cerevisiae S288C
Product	Ent5p
Comment	Comment: REVIEWED REFSEQ: This record has been curated by SGD. This record is derived from an annotated genomic sequence (NC_001136). On Jul 30, 2012 this sequence version replaced NM_001180460.2. ##Genome-Annotation-Data-START## Annotation Provider :: SGD Annotation Status :: Full Annotation Annotation Version :: R64-2-1 URL :: http://www.yeastgenome.org/ ##Genome-Annotation-Data-END## COMPLETENESS: incomplete on both ends.

ATGGACTCAT TATCAAAAAA GATTCAAAAT TTAGGTATCC ATGATATTAG AAATGCCGCA 
AGGTTTGCCC AAAACGTGAT AGTCCAATAT GAGCCTTATC AGATAGATAT CCGTCGTGCT 
ACAAATACCG ATGCTTGGGG CCCCACACCG AAGCACCTCG CCAAGGTTTT AAGGAACAGA 
TACCAAGTGC CGCTCTACTT GATGACAGAA TACACGTTGA AAAGATTAGT CGACCATATC 
GCTACCAGGC CCAAGAACCT ATACGAAAAG GCAAGAAAGG ACTACGTTAA TTATGGGTCC 
GAATGGAGGG TGGTCTTAAA ATGTCTAGTA GTCATTGAAT TCCTATTATT GAACGTCGAC 
ACAGGTGACG AACTGAATCA GATTAGATCA TGTTTGCTCA CTCATAAGCA TATTTTAACC 
AGGGAGATTG CCCAATTCAA GGTAAAATTC TCCAATGATG GTAAAATGGA GATTCATGAG 
AGAGGTATTA GGAAAAAGGG AGAACTTATC TTGCAATACT TGGAAGACTC ACAATTTTTG 
AAAAAGGAAA GAGCCAAGAA TAAGAAGAAT GCCTTGAAAA TTCGACAGCA AGGAGAATCC 
TCCATTTATA ATGCCAACCA GATTTCAACG TCTGCAAGTT ACGATAACAT CGACGACGAT 
GAATTTGATG CAGATGCAGA TGGCTTTGAC AGTGAAATGG ATGCTAATAA TGTGACCAAC 
TTCAATGTTC CTGTGGAAAC AGAAGCAAAT TCGAATACTC GCAGGCGTTC TCATATGGAA 
GAACAAAGAA GACAAAGAAG GGAGATATTA AGAGAGCAGA TCAAAAATAA AGAACAGCAG 
AGGAAAAGAA AGCAACAACA AGATAGCATC CCTGACTTAA TCGATCTTGA CGATAGTACT 
AGCACCACGA ATAATATAAC AATAGACAAT GGTAATAATG ACAACAAAAA CAATAATATC 
AACAGCAATA GTGATGATGA TGATGATGAG TTTGGCGACT TCCAAAGCGA AACGAGTCCC 
GACACAACAG CACCTAAAAC TTCCAATAGT AAAATAGATG ATTTGCTTGA CTGGGATGGC 
CCAAAGTCAG ACACAGATAC CACCGCTGCT GCGCAAACAT CATTGCCCTT TGCGGAGAAA 
AAACAGCAAA AAGCCCGTCC TCAGGCTACA AAGGACAAGT CGAAGGGAAA TGATGCATTT 
TCCGACTTAT TCTCTTACTC CAAGTCGTTG GTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_010437.3
CDS	1..1236
Translation	MDSLSKKIQNLGIHDIRNAARFAQNVIVQYEPYQIDIRRATNTDAWGPTPKHLAKVLRNRYQVPLYLMTEYTLKRLVDHIATRPKNLYEKARKDYVNYGSEWRVVLKCLVVIEFLLLNVDTGDELNQIRSCLLTHKHILTREIAQFKVKFSNDGKMEIHERGIRKKGELILQYLEDSQFLKKERAKNKKNALKIRQQGESSIYNANQISTSASYDNIDDDEFDADADGFDSEMDANNVTNFNVPVETEANSNTRRRSHMEEQRRQRREILREQIKNKEQQRKRKQQQDSIPDLIDLDDSTSTTNNITIDNGNNDNKNNNINSNSDDDDDEFGDFQSETSPDTTAPKTSNSKIDDLLDWDGPKSDTDTTAAAQTSLPFAEKKQQKARPQATKDKSKGNDAFSDLFSYSKSLV

Target ORF information:

RefSeq Version	NM_001180460.3
Organism	Saccharomyces cerevisiae S288C
Definition	Saccharomyces cerevisiae S288C Ent5p (ENT5), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001180460.3

ORF Insert Sequence:

ATGGACTCAT TATCAAAAAA GATTCAAAAT TTAGGTATCC ATGATATTAG AAATGCCGCA 
AGGTTTGCCC AAAACGTGAT AGTCCAATAT GAGCCTTATC AGATAGATAT CCGTCGTGCT 
ACAAATACCG ATGCTTGGGG CCCCACACCG AAGCACCTCG CCAAGGTTTT AAGGAACAGA 
TACCAAGTGC CGCTCTACTT GATGACAGAA TACACGTTGA AAAGATTAGT CGACCATATC 
GCTACCAGGC CCAAGAACCT ATACGAAAAG GCAAGAAAGG ACTACGTTAA TTATGGGTCC 
GAATGGAGGG TGGTCTTAAA ATGTCTAGTA GTCATTGAAT TCCTATTATT GAACGTCGAC 
ACAGGTGACG AACTGAATCA GATTAGATCA TGTTTGCTCA CTCATAAGCA TATTTTAACC 
AGGGAGATTG CCCAATTCAA GGTAAAATTC TCCAATGATG GTAAAATGGA GATTCATGAG 
AGAGGTATTA GGAAAAAGGG AGAACTTATC TTGCAATACT TGGAAGACTC ACAATTTTTG 
AAAAAGGAAA GAGCCAAGAA TAAGAAGAAT GCCTTGAAAA TTCGACAGCA AGGAGAATCC 
TCCATTTATA ATGCCAACCA GATTTCAACG TCTGCAAGTT ACGATAACAT CGACGACGAT 
GAATTTGATG CAGATGCAGA TGGCTTTGAC AGTGAAATGG ATGCTAATAA TGTGACCAAC 
TTCAATGTTC CTGTGGAAAC AGAAGCAAAT TCGAATACTC GCAGGCGTTC TCATATGGAA 
GAACAAAGAA GACAAAGAAG GGAGATATTA AGAGAGCAGA TCAAAAATAA AGAACAGCAG 
AGGAAAAGAA AGCAACAACA AGATAGCATC CCTGACTTAA TCGATCTTGA CGATAGTACT 
AGCACCACGA ATAATATAAC AATAGACAAT GGTAATAATG ACAACAAAAA CAATAATATC 
AACAGCAATA GTGATGATGA TGATGATGAG TTTGGCGACT TCCAAAGCGA AACGAGTCCC 
GACACAACAG CACCTAAAAC TTCCAATAGT AAAATAGATG ATTTGCTTGA CTGGGATGGC 
CCAAAGTCAG ACACAGATAC CACCGCTGCT GCGCAAACAT CATTGCCCTT TGCGGAGAAA 
AAACAGCAAA AAGCCCGTCC TCAGGCTACA AAGGACAAGT CGAAGGGAAA TGATGCATTT 
TCCGACTTAT TCTCTTACTC CAAGTCGTTG GTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The nucleotide sequence of Saccharomyces cerevisiae chromosome IV.
Nature387(6632 Suppl)75-8(1997 May)
Jacq C,Alt-M?rbe J,Andre B,Arnold W,Bahr A,Ballesta JP,Bargues M,Baron L,Becker A,Biteau N,Bl?cker H,Blugeon C,Boskovic J,Brandt P,Br?ckner M,Buitrago MJ,Coster F,Delaveau T,del Rey F,Dujon B,Eide LG,Garcia-Cantalejo JM,Goffeau A,Gomez-Peris A,Zaccaria P

Life with 6000 genes.
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)
Goffeau A,Barrell BG,Bussey H,Davis RW,Dujon B,Feldmann H,Galibert F,Hoheisel JD,Jacq C,Johnston M,Louis EJ,Mewes HW,Murakami Y,Philippsen P,Tettelin H,Oliver SG

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ENT5 cDNA ORF clone, Saccharomyces cerevisiae S288C

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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