GLT1 cDNA ORF clone, Saccharomyces cerevisiae S288C

Gene
Clones
gRNAs

Summary

Gene Symbol	GLT1
Entrez Gene ID	851383
Full Name	glutamate synthase (NADH)
Gene Type	protein-coding
Organism	Saccharomyces cerevisiae S288C

mRNA and Protein(s)

mRNA	Protein	Name
NM_001180231.1	NP_010110.1	glutamate synthase (NADH)

Pathways from BioSystems

Homologies

Drosophila melanogaster (fruit fly)	CG9674	NP_001246804.1
Schizosaccharomyces pombe (fission yeast)	glt1	NP_594133.1
Magnaporthe oryzae (rice blast fungus)	MGG_07187	XP_003715392.1
Neurospora crassa	NCU01744	XP_957167.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP006360	XP_316385.4
Caenorhabditis elegans (roundworm)	W07E11.1	NP_509693.2
Kluyveromyces lactis	KLLA0E19625g	XP_454839.1
Eremothecium gossypii	AGOS_ADR290W	NP_984386.2
Arabidopsis thaliana (thale cress)	GLT1	NP_200158.2
Oryza sativa (rice)	Os01g0681900	NP_001043884.1
Saccharomyces cerevisiae (baker's yeast)	GLT1	NP_010110.1

Gene Ontology
Bibliography

Related articles in PubMed

The nucleotide sequence of Saccharomyces cerevisiae chromosome IV.
Jacq C, Alt-M?rbe J, Andre B, Arnold W, Bahr A, Ballesta JP, Bargues M, Baron L, Becker A, Biteau N, Bl?cker H, Blugeon C, Boskovic J, Brandt P, Br?ckner M, Buitrago MJ, Coster F, Delaveau T, del Rey F, Dujon B, Eide LG, Garcia-Cantalejo JM, Goffeau A, Gomez-Peris A, Zaccaria P
Nature387(6632 Suppl)75-8(1997 May)

Life with 6000 genes.
Goffeau A, Barrell BG, Bussey H, Davis RW, Dujon B, Feldmann H, Galibert F, Hoheisel JD, Jacq C, Johnston M, Louis EJ, Mewes HW, Murakami Y, Philippsen P, Tettelin H, Oliver SG
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Over-expressing the GLT1 gene in the presence of a glycerolphosphate dehydrogenase deletion increases ethanol production.
Title: Over-expressing GLT1 in a gpd2Delta mutant of Saccharomyces cerevisiae to improve ethanol production.

Improved production of ethanol by deleting FPS1 and over-expressing GLT1 in Saccharomyces cerevisiae.
Title: Improved production of ethanol by deleting FPS1 and over-expressing GLT1 in Saccharomyces cerevisiae.

Chromatin organization plays a major role in the bidirectional properties of the UGA3-GLT1 promoter, through the action of an upstream Abf1p-binding consensus sequence and a polydAdT(tract).
Title: The UGA3-GLT1 intergenic region constitutes a promoter whose bidirectional nature is determined by chromatin organization in Saccharomyces cerevisiae.

The following GLT1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the GLT1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OSi00938	NM_001180231.1 Latest version!	Saccharomyces cerevisiae S288C glutamate synthase (NADH) (GLT1), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1161.30 $1659.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

GLT1 ( NM_001180231.1 ) cDNA ORF clone, Saccharomyces cerevisiae S288C -> NP_010110.1 Saccharomyces cerevisiae S288C glutamate synthase (NADH) (GLT1), partial mRNA.

Price & Availability↑

CloneID	OSi00938
Clone ID Related Accession (Same CDS sequence)	NM_001180231.1
Accession Version	NM_001180231.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6438bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-31
Organism	Saccharomyces cerevisiae S288C
Product	glutamate synthase (NADH)
Comment	Comment: REVIEWED REFSEQ: This record has been curated by SGD. This record is derived from an annotated genomic sequence (NC_001136). ##Genome-Annotation-Data-START## Annotation Provider :: SGD Annotation Status :: Full Annotation Annotation Version :: R64-2-1 URL :: http://www.yeastgenome.org/ ##Genome-Annotation-Data-END## COMPLETENESS: incomplete on both ends.

ATGCCAGTGT TGAAATCAGA CAATTTCGAT CCATTGGAAG AAGCTTACGA AGGTGGGACA 
ATTCAAAACT ATAACGATGA ACACCATCTT CATAAATCTT GGGCAAATGT GATTCCGGAC 
AAACGAGGAC TTTACGACCC TGATTATGAA CATGACGCTT GTGGTGTCGG TTTCGTAGCA 
AATAAGCATG GTGAACAGTC TCACAAGATT GTTACTGACG CTAGATATCT TTTAGTGAAT 
ATGACACATC GTGGTGCCGT CTCATCTGAT GGGAACGGTG ACGGTGCCGG TATTCTGCTA 
GGTATTCCTC ACGAATTTAT GAAAAGAGAA TTCAAGTTAG ATCTTGATCT AGACATACCT 
GAGATGGGCA AATACGCCGT AGGTAACGTC TTCTTCAAGA AGAACGAAAA AAATAACAAG 
AAAAATTTAA TTAAGTGTCA GAAGATTTTC GAGGATTTAG CTGCATCCTT CAACTTATCC 
GTATTAGGTT GGAGAAACGT CCCCGTAGAT TCTACTATTT TAGGAGACGT TGCATTATCT 
CGTGAACCTA CTATTCTACA GCCATTATTG GTTCCATTGT ATGATGAAAA ACAACCGGAG 
TTTAATGAAA CTAAATTTAG AACTCAATTG TATCTTTTAA GGAAGGAGGC CTCTCTTCAA 
ATAGGACTGG AAAACTGGTT CTATGTTTGT TCCCTAAACA ATACCACCAT TGTTTACAAG 
GGTCAATTGA CGCCAGCTCA AGTGTATAAC TACTATCCCG ACTTGACTAA TGCGCATTTC 
AAATCCCACA TGGCGTTGGT CCATTCAAGA TTTTCCACTA ATACTTTCCC CTCTTGGGAT 
AGAGCTCAAC CTTTACGTTG GCTAGCTCAT AATGGTGAAA TTAACACCTT AAGAGGTAAC 
AAGAATTGGA TGCGCTCCAG AGAAGGTGTG ATGAATTCAG CAACTTTCAA AGATGAGTTA 
GACAAACTAT ACCCAATTAT CGAAGAAGGT GGTTCTGATT CAGCTGCATT GGATAACGTT 
TTAGAACTAT TGACTATTAA TGGCACATTA TCTCTACCTG AAGCTGTTAT GATGATGGTT 
CCTGAAGCGT ATCATAAGGA TATGGATTCT GACCTAAAAG CATGGTACGA CTGGGCTGCA 
TGTCTGATGG AACCTTGGGA TGGTCCAGCT TTGTTAACTT TCACTGATGG ACGTTACTGT 
GGTGCTATAT TGGATAGAAA TGGTTTAAGA CCTTGTCGTT ATTACATCAC TAGTGATGAC 
AGAGTTATCT GTGCTTCAGA GGTAGGTGTC ATTCCTATCG AAAATTCATT GGTTGTTCAA 
AAAGGTAAAC TGAAGCCAGG TGATTTATTC CTAGTGGATA CTCAATTGGG TGAAATGGTC 
GATACTAAAA AGTTAAAATC TCAAATCTCA AAAAGACAAG ATTTTAAGTC TTGGTTATCC 
AAAGTCATCA AGTTAGACGA CTTGTTATCA AAAACCGCTA ATTTGGTTCC TAAAGAATTT 
ATATCACAGG ATTCATTGTC TTTGAAAGTT CAAAGTGACC CACGTCTATT GGCCAATGGT 
TATACCTTCG AACAAGTCAC ATTTCTGTTA ACTCCAATGG CTTTAACAGG TAAAGAAGCT 
TTAGGTTCGA TGGGTAACGA TGCGCCACTG GCTTGTTTAA ATGAAAATCC TGTCTTACTT 
TATGATTATT TCAGACAATT GTTTGCTCAA GTGACCAATC CTCCAATTGA CCCAATTCGT 
GAAGCAAATG TTATGTCGTT AGAATGTTAT GTCGGACCTC AAGGCAACCT TTTGGAAATG 
CATTCATCTC AATGTGATCG TTTATTATTG AAATCTCCTA TTTTGCATTG GAATGAGTTC 
CAAGCTTTGA AAAACATTGA AGCTGCTTAC CCATCATGGT CTGTAGCAGA AATTGATATC 
ACATTCGACA AGAGTGAGGG TCTATTGGGC TATACCGACA CAATTGATAA AATCACTAAG 
TTAGCGAGCG AAGCAATTGA TGATGGTAAA AAGATCTTAA TAATTACTGA CAGGAAAATG 
GGTGCCAACC GTGTTTCCAT CTCCTCTTTG ATTGCAATTT CATGTATTCA TCATCACCTA 
ATCAGAAACA AGCAGCGTTC CCAAGTTGCT TTGATTTTGG AAACAGGTGA AGCCAGAGAA 
ATTCACCATT TCTGTGTCCT ACTAGGTTAT GGTTGTGATG GTGTTTATCC ATACTTAGCC 
ATGGAAACTT TGGTCAGAAT GAATAGAGAA GGTCTACTTC GTAATGTCAA CAATGACAAT 
GATACACTTG AGGAAGGGCA AATACTAGAA AATTACAAGC ACGCTATTGA TGCAGGTATC 
TTGAAGGTTA TGTCTAAAAT GGGTATCTCC ACTCTAGCAT CCTACAAAGG TGCTCAAATT 
TTTGAAGCCC TAGGTTTAGA TAACTCTATT GTTGATTTGT GTTTCACAGG TACTTCTTCC 
AGAATTAGAG GTGTAACTTT CGAGTATTTG GCTCAAGATG CCTTTTCTTT ACATGAGCGT 
GGTTATCCAT CCAGACAAAC CATTAGTAAA TCTGTTAACT TACCAGAAAG TGGTGAATAC 
CACTTTAGGG ATGGTGGTTA CAAACACGTC AACGAACCAA CCGCAATTGC TTCGTTACAA 
GATACTGTCA GAAACAAAAA TGATGTCTCT TGGCAATTAT ATGTAAAGAA GGAAATGGAA 
GCAATTAGAG ACTGTACACT AAGAGGACTG TTAGAATTAG ATTTTGAAAA TTCTGTCAGT 
ATCCCTCTAG AACAAGTTGA ACCATGGACT GAAATTGCCA GAAGATTTGC GTCAGGTGCA 
ATGTCTTATG GTTCTATTTC TATGGAAGCT CACTCTACAT TGGCTATTGC CATGAATCGT 
TTAGGGGCCA AATCCAATTG TGGTGAAGGT GGTGAAGACG CAGAACGTTC TGCTGTTCAA 
GAAAACGGTG ATACTATGAG ATCTGCTATC AAACAAGTTG CTTCCGCTAG ATTCGGTGTA 
ACTTCATACT ACTTGTCAGA TGCTGATGAA ATCCAAATTA AGATTGCTCA GGGTGCTAAG 
CCGGGTGAAG GTGGTGAACT ACCAGCCCAC AAAGTGTCTA AGGATATCGC AAAAACCAGG 
CACTCCACCC CTAATGTTGG GTTAATCTCT CCTCCTCCTC ATCACGATAT TTATTCCATT 
GAAGATTTGA AACAACTGAT TTATGATTTG AAATGTGCTA ATCCAAGAGC GGGAATTTCT 
GTAAAGTTGG TTTCCGAAGT TGGTGTTGGT ATTGTTGCCT CTGGTGTAGC TAAGGCTAAA 
GCCGATCATA TCTTAGTTTC TGGTCATGAT GGTGGTACAG GTGCTGCAAG ATGGACGAGT 
GTCAAATATG CGGGTTTGCC ATGGGAATTA GGTCTAGCTG AAACTCACCA GACTTTAGTC 
TTGAATGATT TAAGACGTAA TGTTGTTGTC CAAACCGATG GTCAATTGAG AACTGGGTTT 
GATATTGCTG TTGCAGTTTT ATTAGGGGCA GAATCTTTTA CCTTGGCAAC AGTTCCATTA 
ATTGCTATGG GTTGTGTTAT GTTAAGAAGA TGTCACTTGA ACTCTTGTGC TGTTGGTATT 
GCCACACAAG ATCCATATTT GAGAAGTAAG TTTAAGGGTC AGCCCGAACA TGTTATCAAC 
TTCTTCTATT ACTTGATCCA AGATTTAAGA CAAATCATGG CCAAGTTAGG ATTCCGTACC 
ATTGACGAAA TGGTGGGTCA TTCTGAAAAA TTAAAGAAAA GGGACGACGT AAATGCCAAA 
GCCATAAATA TCGATTTATC TCCTATTTTG ACCCCAGCAC ATGTTATTCG TCCAGGTGTT 
CCAACCAAGT TCACTAAGAA ACAAGACCAC AAACTCCACA CCCGTCTAGA TAATAAGTTA 
ATCGATGAGG CTGAAGTTAC TTTGGATCGT GGCTTACCAG TGAATATTGA CGCCTCTATA 
ATCAATACTG ATCGTGCACT CGGTTCTACT TTATCTTACA GAGTCTCGAA GAAATTTGGT 
GAAGATGGTT TGCCAAAGGA CACCGTTGTC GTTAACATAG AAGGTTCAGC GGGTCAATCT 
TTTGGTGCTT TCCTAGCTTC TGGTATCACT TTTATCTTGA ATGGTGATGC TAATGATTAT 
GTTGGTAAAG GTTTATCCGG TGGTATTATT GTCATTAAAC CACCAAAGGA TTCTAAATTC 
AAGAGTGATG AAAATGTAAT TGTTGGTAAC ACTTGTTTCT ATGGTGCTAC TTCTGGTACT 
GCATTCATTT CAGGTAGTGC CGGTGAGCGT TTCGGTGTCA GAAACTCTGG TGCCACCATC 
GTTGTTGAGA GAATTAAGGG TAACAATGCC TTTGAGTATA TGACTGGTGG TCGTGCCATT 
GTCTTATCAC AAATGGAATC CCTAAACGCC TTCTCTGGTG CTACTGGTGG TATTGCATAC 
TGTTTAACTT CCGATTACGA CGATTTTGTT GGAAAGATTA ACAAAGATAC TGTTGAGTTA 
GAATCATTAT GTGACCCGGT CGAGATTGCG TTTGTTAAGA ATTTGATCCA GGAGCATTGG 
AACTACACAC AATCTGATCT AGCAGCCAGG ATTCTCGGTA ATTTCAACCA TTATTTGAAA 
GATTTCGTTA AAGTCATTCC AACTGATTAT AAGAAAGTTT TGTTGAAGGA GAAAGCAGAA 
GCTGCCAAGG CAAAGGCTAA GGCAACTTCA GAATACTTAA AGAAGTTTAG ATCGAACCAA 
GAAGTTGATG ACGAAGTCAA TACTCTATTG ATTGCTAATC AAAAAGCTAA AGAGCAAGAA 
AAAAAGAAGA GTATTACTAT TTCAAATAAG GCCACTTTGA AGGAGCCTAA GGTTGTTGAT 
TTAGAAGATG CAGTTCCAGA TTCCAAACAG CTAGAGAAGA ATAGCGAAAG GATTGAAAAA 
ACACGTGGTT TTATGATCCA CAAACGTCGT CATGAGACAC ACAGAGATCC AAGAACCAGA 
GTTAATGACT GGAAAGAATT TACTAACCCT ATTACCAAGA AGGATGCCAA ATATCAAACT 
GCGAGATGTA TGGATTGTGG TACACCATTC TGTTTATCTG ATACCGGTTG TCCCCTATCT 
AACATTATCC CCAAGTTTAA TGAATTGTTA TTCAAGAACC AATGGAAGTT GGCACTGGAC 
AAATTGCTAG AGACAAACAA TTTCCCAGAA TTCACTGGAA GAGTATGTCC AGCACCCTGT 
GAGGGAGCTT GTACACTAGG TATTATTGAA GACCCAGTCG GCATAAAATC GGTTGAAAGA 
ATTATCATTG ACAATGCTTT CAAGGAAGGA TGGATTAAGC CTTGTCCACC AAGTACACGC 
ACTGGCTTTA CAGTGGGTGT CATTGGTTCT GGTCCAGCAG GTTTAGCGTG TGCTGATATG 
TTGAACCGTG CCGGACATAC GGTCACTGTT TATGAAAGAT CCGACCGTTG TGGTGGGTTA 
TTGATGTATG GTATTCCAAA CATGAAGTTG GATAAGGCTA TAGTGCAACG TCGTATTGAT 
CTATTGAGTG CCGAAGGTAT TGACTTTGTT ACCAACACCG AAATTGGTAA AACCATAAGC 
ATGGATGAGC TAAAGAACAA GCACAATGCA GTAGTGTATG CTATCGGTTC TACCATTCCA 
CGTGACTTAC CTATTAAGGG TCGTGAATTG AAGAATATTG ATTTTGCCAT GCAGTTGTTG 
GAATCTAACA CAAAAGCTTT ATTGAACAAA GATCTGGAAA TCATTCGTGA AAAGATCCAA 
GGTAAGAAAG TAATTGTTGT CGGTGGTGGT GACACAGGTA ACGATTGTTT AGGTACATCT 
GTAAGACACG GTGCAGCATC AGTTTTGAAT TTCGAATTGT TGCCTGAGCC ACCAGTGGAA 
CGTGCCAAAG ACAATCCATG GCCTCAATGG CCGCGTGTCA TGAGAGTGGA CTACGGTCAT 
GCTGAAGTGA AAGAGCATTA TGGTAGAGAC CCTCGTGAAT ACTGCATCTT GTCCAAGGAA 
TTTATCGGTA ACGATGAGGG TGAAGTCACT GCCATCAGAA CTGTGCGCGT AGAATGGAAG 
AAGTCACAAA GTGGCGTATG GCAAATGGTA GAAATTCCCA ACAGTGAAGA GATCTTTGAA 
GCCGATATCA TTTTGTTGTC TATGGGTTTC GTGGGTCCTG AATTGATCAA TGGCAACGAT 
AACGAAGTTA AGAAGACAAG ACGTGGTACG ATTGCCACAC TCGACGACTC CTCATACTCT 
ATTGATGGAG GAAAGACTTT TGCATGTGGT GACTGTAGAA GAGGGCAATC TTTGATTGTC 
TGGGCCATCC AAGAAGGTAG AAAATGTGCT GCCTCTGTCG ATAAGTTCCT AATGGACGGC 
ACTACGTATC TACCAAGTAA TGGTGGTATC GTTCAACGTG ATTACAAACT ATTGAAAGAA 
TTAGCTAGTC AAGTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_010110.1
CDS	1..6438
Translation	MPVLKSDNFDPLEEAYEGGTIQNYNDEHHLHKSWANVIPDKRGLYDPDYEHDACGVGFVANKHGEQSHKIVTDARYLLVNMTHRGAVSSDGNGDGAGILLGIPHEFMKREFKLDLDLDIPEMGKYAVGNVFFKKNEKNNKKNLIKCQKIFEDLAASFNLSVLGWRNVPVDSTILGDVALSREPTILQPLLVPLYDEKQPEFNETKFRTQLYLLRKEASLQIGLENWFYVCSLNNTTIVYKGQLTPAQVYNYYPDLTNAHFKSHMALVHSRFSTNTFPSWDRAQPLRWLAHNGEINTLRGNKNWMRSREGVMNSATFKDELDKLYPIIEEGGSDSAALDNVLELLTINGTLSLPEAVMMMVPEAYHKDMDSDLKAWYDWAACLMEPWDGPALLTFTDGRYCGAILDRNGLRPCRYYITSDDRVICASEVGVIPIENSLVVQKGKLKPGDLFLVDTQLGEMVDTKKLKSQISKRQDFKSWLSKVIKLDDLLSKTANLVPKEFISQDSLSLKVQSDPRLLANGYTFEQVTFLLTPMALTGKEALGSMGNDAPLACLNENPVLLYDYFRQLFAQVTNPPIDPIREANVMSLECYVGPQGNLLEMHSSQCDRLLLKSPILHWNEFQALKNIEAAYPSWSVAEIDITFDKSEGLLGYTDTIDKITKLASEAIDDGKKILIITDRKMGANRVSISSLIAISCIHHHLIRNKQRSQVALILETGEAREIHHFCVLLGYGCDGVYPYLAMETLVRMNREGLLRNVNNDNDTLEEGQILENYKHAIDAGILKVMSKMGISTLASYKGAQIFEALGLDNSIVDLCFTGTSSRIRGVTFEYLAQDAFSLHERGYPSRQTISKSVNLPESGEYHFRDGGYKHVNEPTAIASLQDTVRNKNDVSWQLYVKKEMEAIRDCTLRGLLELDFENSVSIPLEQVEPWTEIARRFASGAMSYGSISMEAHSTLAIAMNRLGAKSNCGEGGEDAERSAVQENGDTMRSAIKQVASARFGVTSYYLSDADEIQIKIAQGAKPGEGGELPAHKVSKDIAKTRHSTPNVGLISPPPHHDIYSIEDLKQLIYDLKCANPRAGISVKLVSEVGVGIVASGVAKAKADHILVSGHDGGTGAARWTSVKYAGLPWELGLAETHQTLVLNDLRRNVVVQTDGQLRTGFDIAVAVLLGAESFTLATVPLIAMGCVMLRRCHLNSCAVGIATQDPYLRSKFKGQPEHVINFFYYLIQDLRQIMAKLGFRTIDEMVGHSEKLKKRDDVNAKAINIDLSPILTPAHVIRPGVPTKFTKKQDHKLHTRLDNKLIDEAEVTLDRGLPVNIDASIINTDRALGSTLSYRVSKKFGEDGLPKDTVVVNIEGSAGQSFGAFLASGITFILNGDANDYVGKGLSGGIIVIKPPKDSKFKSDENVIVGNTCFYGATSGTAFISGSAGERFGVRNSGATIVVERIKGNNAFEYMTGGRAIVLSQMESLNAFSGATGGIAYCLTSDYDDFVGKINKDTVELESLCDPVEIAFVKNLIQEHWNYTQSDLAARILGNFNHYLKDFVKVIPTDYKKVLLKEKAEAAKAKAKATSEYLKKFRSNQEVDDEVNTLLIANQKAKEQEKKKSITISNKATLKEPKVVDLEDAVPDSKQLEKNSERIEKTRGFMIHKRRHETHRDPRTRVNDWKEFTNPITKKDAKYQTARCMDCGTPFCLSDTGCPLSNIIPKFNELLFKNQWKLALDKLLETNNFPEFTGRVCPAPCEGACTLGIIEDPVGIKSVERIIIDNAFKEGWIKPCPPSTRTGFTVGVIGSGPAGLACADMLNRAGHTVTVYERSDRCGGLLMYGIPNMKLDKAIVQRRIDLLSAEGIDFVTNTEIGKTISMDELKNKHNAVVYAIGSTIPRDLPIKGRELKNIDFAMQLLESNTKALLNKDLEIIREKIQGKKVIVVGGGDTGNDCLGTSVRHGAASVLNFELLPEPPVERAKDNPWPQWPRVMRVDYGHAEVKEHYGRDPREYCILSKEFIGNDEGEVTAIRTVRVEWKKSQSGVWQMVEIPNSEEIFEADIILLSMGFVGPELINGNDNEVKKTRRGTIATLDDSSYSIDGGKTFACGDCRRGQSLIVWAIQEGRKCAASVDKFLMDGTTYLPSNGGIVQRDYKLLKELASQV

Target ORF information:

RefSeq Version	NM_001180231.1
Organism	Saccharomyces cerevisiae S288C
Definition	Saccharomyces cerevisiae S288C glutamate synthase (NADH) (GLT1), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001180231.1

ORF Insert Sequence:

ATGCCAGTGT TGAAATCAGA CAATTTCGAT CCATTGGAAG AAGCTTACGA AGGTGGGACA 
ATTCAAAACT ATAACGATGA ACACCATCTT CATAAATCTT GGGCAAATGT GATTCCGGAC 
AAACGAGGAC TTTACGACCC TGATTATGAA CATGACGCTT GTGGTGTCGG TTTCGTAGCA 
AATAAGCATG GTGAACAGTC TCACAAGATT GTTACTGACG CTAGATATCT TTTAGTGAAT 
ATGACACATC GTGGTGCCGT CTCATCTGAT GGGAACGGTG ACGGTGCCGG TATTCTGCTA 
GGTATTCCTC ACGAATTTAT GAAAAGAGAA TTCAAGTTAG ATCTTGATCT AGACATACCT 
GAGATGGGCA AATACGCCGT AGGTAACGTC TTCTTCAAGA AGAACGAAAA AAATAACAAG 
AAAAATTTAA TTAAGTGTCA GAAGATTTTC GAGGATTTAG CTGCATCCTT CAACTTATCC 
GTATTAGGTT GGAGAAACGT CCCCGTAGAT TCTACTATTT TAGGAGACGT TGCATTATCT 
CGTGAACCTA CTATTCTACA GCCATTATTG GTTCCATTGT ATGATGAAAA ACAACCGGAG 
TTTAATGAAA CTAAATTTAG AACTCAATTG TATCTTTTAA GGAAGGAGGC CTCTCTTCAA 
ATAGGACTGG AAAACTGGTT CTATGTTTGT TCCCTAAACA ATACCACCAT TGTTTACAAG 
GGTCAATTGA CGCCAGCTCA AGTGTATAAC TACTATCCCG ACTTGACTAA TGCGCATTTC 
AAATCCCACA TGGCGTTGGT CCATTCAAGA TTTTCCACTA ATACTTTCCC CTCTTGGGAT 
AGAGCTCAAC CTTTACGTTG GCTAGCTCAT AATGGTGAAA TTAACACCTT AAGAGGTAAC 
AAGAATTGGA TGCGCTCCAG AGAAGGTGTG ATGAATTCAG CAACTTTCAA AGATGAGTTA 
GACAAACTAT ACCCAATTAT CGAAGAAGGT GGTTCTGATT CAGCTGCATT GGATAACGTT 
TTAGAACTAT TGACTATTAA TGGCACATTA TCTCTACCTG AAGCTGTTAT GATGATGGTT 
CCTGAAGCGT ATCATAAGGA TATGGATTCT GACCTAAAAG CATGGTACGA CTGGGCTGCA 
TGTCTGATGG AACCTTGGGA TGGTCCAGCT TTGTTAACTT TCACTGATGG ACGTTACTGT 
GGTGCTATAT TGGATAGAAA TGGTTTAAGA CCTTGTCGTT ATTACATCAC TAGTGATGAC 
AGAGTTATCT GTGCTTCAGA GGTAGGTGTC ATTCCTATCG AAAATTCATT GGTTGTTCAA 
AAAGGTAAAC TGAAGCCAGG TGATTTATTC CTAGTGGATA CTCAATTGGG TGAAATGGTC 
GATACTAAAA AGTTAAAATC TCAAATCTCA AAAAGACAAG ATTTTAAGTC TTGGTTATCC 
AAAGTCATCA AGTTAGACGA CTTGTTATCA AAAACCGCTA ATTTGGTTCC TAAAGAATTT 
ATATCACAGG ATTCATTGTC TTTGAAAGTT CAAAGTGACC CACGTCTATT GGCCAATGGT 
TATACCTTCG AACAAGTCAC ATTTCTGTTA ACTCCAATGG CTTTAACAGG TAAAGAAGCT 
TTAGGTTCGA TGGGTAACGA TGCGCCACTG GCTTGTTTAA ATGAAAATCC TGTCTTACTT 
TATGATTATT TCAGACAATT GTTTGCTCAA GTGACCAATC CTCCAATTGA CCCAATTCGT 
GAAGCAAATG TTATGTCGTT AGAATGTTAT GTCGGACCTC AAGGCAACCT TTTGGAAATG 
CATTCATCTC AATGTGATCG TTTATTATTG AAATCTCCTA TTTTGCATTG GAATGAGTTC 
CAAGCTTTGA AAAACATTGA AGCTGCTTAC CCATCATGGT CTGTAGCAGA AATTGATATC 
ACATTCGACA AGAGTGAGGG TCTATTGGGC TATACCGACA CAATTGATAA AATCACTAAG 
TTAGCGAGCG AAGCAATTGA TGATGGTAAA AAGATCTTAA TAATTACTGA CAGGAAAATG 
GGTGCCAACC GTGTTTCCAT CTCCTCTTTG ATTGCAATTT CATGTATTCA TCATCACCTA 
ATCAGAAACA AGCAGCGTTC CCAAGTTGCT TTGATTTTGG AAACAGGTGA AGCCAGAGAA 
ATTCACCATT TCTGTGTCCT ACTAGGTTAT GGTTGTGATG GTGTTTATCC ATACTTAGCC 
ATGGAAACTT TGGTCAGAAT GAATAGAGAA GGTCTACTTC GTAATGTCAA CAATGACAAT 
GATACACTTG AGGAAGGGCA AATACTAGAA AATTACAAGC ACGCTATTGA TGCAGGTATC 
TTGAAGGTTA TGTCTAAAAT GGGTATCTCC ACTCTAGCAT CCTACAAAGG TGCTCAAATT 
TTTGAAGCCC TAGGTTTAGA TAACTCTATT GTTGATTTGT GTTTCACAGG TACTTCTTCC 
AGAATTAGAG GTGTAACTTT CGAGTATTTG GCTCAAGATG CCTTTTCTTT ACATGAGCGT 
GGTTATCCAT CCAGACAAAC CATTAGTAAA TCTGTTAACT TACCAGAAAG TGGTGAATAC 
CACTTTAGGG ATGGTGGTTA CAAACACGTC AACGAACCAA CCGCAATTGC TTCGTTACAA 
GATACTGTCA GAAACAAAAA TGATGTCTCT TGGCAATTAT ATGTAAAGAA GGAAATGGAA 
GCAATTAGAG ACTGTACACT AAGAGGACTG TTAGAATTAG ATTTTGAAAA TTCTGTCAGT 
ATCCCTCTAG AACAAGTTGA ACCATGGACT GAAATTGCCA GAAGATTTGC GTCAGGTGCA 
ATGTCTTATG GTTCTATTTC TATGGAAGCT CACTCTACAT TGGCTATTGC CATGAATCGT 
TTAGGGGCCA AATCCAATTG TGGTGAAGGT GGTGAAGACG CAGAACGTTC TGCTGTTCAA 
GAAAACGGTG ATACTATGAG ATCTGCTATC AAACAAGTTG CTTCCGCTAG ATTCGGTGTA 
ACTTCATACT ACTTGTCAGA TGCTGATGAA ATCCAAATTA AGATTGCTCA GGGTGCTAAG 
CCGGGTGAAG GTGGTGAACT ACCAGCCCAC AAAGTGTCTA AGGATATCGC AAAAACCAGG 
CACTCCACCC CTAATGTTGG GTTAATCTCT CCTCCTCCTC ATCACGATAT TTATTCCATT 
GAAGATTTGA AACAACTGAT TTATGATTTG AAATGTGCTA ATCCAAGAGC GGGAATTTCT 
GTAAAGTTGG TTTCCGAAGT TGGTGTTGGT ATTGTTGCCT CTGGTGTAGC TAAGGCTAAA 
GCCGATCATA TCTTAGTTTC TGGTCATGAT GGTGGTACAG GTGCTGCAAG ATGGACGAGT 
GTCAAATATG CGGGTTTGCC ATGGGAATTA GGTCTAGCTG AAACTCACCA GACTTTAGTC 
TTGAATGATT TAAGACGTAA TGTTGTTGTC CAAACCGATG GTCAATTGAG AACTGGGTTT 
GATATTGCTG TTGCAGTTTT ATTAGGGGCA GAATCTTTTA CCTTGGCAAC AGTTCCATTA 
ATTGCTATGG GTTGTGTTAT GTTAAGAAGA TGTCACTTGA ACTCTTGTGC TGTTGGTATT 
GCCACACAAG ATCCATATTT GAGAAGTAAG TTTAAGGGTC AGCCCGAACA TGTTATCAAC 
TTCTTCTATT ACTTGATCCA AGATTTAAGA CAAATCATGG CCAAGTTAGG ATTCCGTACC 
ATTGACGAAA TGGTGGGTCA TTCTGAAAAA TTAAAGAAAA GGGACGACGT AAATGCCAAA 
GCCATAAATA TCGATTTATC TCCTATTTTG ACCCCAGCAC ATGTTATTCG TCCAGGTGTT 
CCAACCAAGT TCACTAAGAA ACAAGACCAC AAACTCCACA CCCGTCTAGA TAATAAGTTA 
ATCGATGAGG CTGAAGTTAC TTTGGATCGT GGCTTACCAG TGAATATTGA CGCCTCTATA 
ATCAATACTG ATCGTGCACT CGGTTCTACT TTATCTTACA GAGTCTCGAA GAAATTTGGT 
GAAGATGGTT TGCCAAAGGA CACCGTTGTC GTTAACATAG AAGGTTCAGC GGGTCAATCT 
TTTGGTGCTT TCCTAGCTTC TGGTATCACT TTTATCTTGA ATGGTGATGC TAATGATTAT 
GTTGGTAAAG GTTTATCCGG TGGTATTATT GTCATTAAAC CACCAAAGGA TTCTAAATTC 
AAGAGTGATG AAAATGTAAT TGTTGGTAAC ACTTGTTTCT ATGGTGCTAC TTCTGGTACT 
GCATTCATTT CAGGTAGTGC CGGTGAGCGT TTCGGTGTCA GAAACTCTGG TGCCACCATC 
GTTGTTGAGA GAATTAAGGG TAACAATGCC TTTGAGTATA TGACTGGTGG TCGTGCCATT 
GTCTTATCAC AAATGGAATC CCTAAACGCC TTCTCTGGTG CTACTGGTGG TATTGCATAC 
TGTTTAACTT CCGATTACGA CGATTTTGTT GGAAAGATTA ACAAAGATAC TGTTGAGTTA 
GAATCATTAT GTGACCCGGT CGAGATTGCG TTTGTTAAGA ATTTGATCCA GGAGCATTGG 
AACTACACAC AATCTGATCT AGCAGCCAGG ATTCTCGGTA ATTTCAACCA TTATTTGAAA 
GATTTCGTTA AAGTCATTCC AACTGATTAT AAGAAAGTTT TGTTGAAGGA GAAAGCAGAA 
GCTGCCAAGG CAAAGGCTAA GGCAACTTCA GAATACTTAA AGAAGTTTAG ATCGAACCAA 
GAAGTTGATG ACGAAGTCAA TACTCTATTG ATTGCTAATC AAAAAGCTAA AGAGCAAGAA 
AAAAAGAAGA GTATTACTAT TTCAAATAAG GCCACTTTGA AGGAGCCTAA GGTTGTTGAT 
TTAGAAGATG CAGTTCCAGA TTCCAAACAG CTAGAGAAGA ATAGCGAAAG GATTGAAAAA 
ACACGTGGTT TTATGATCCA CAAACGTCGT CATGAGACAC ACAGAGATCC AAGAACCAGA 
GTTAATGACT GGAAAGAATT TACTAACCCT ATTACCAAGA AGGATGCCAA ATATCAAACT 
GCGAGATGTA TGGATTGTGG TACACCATTC TGTTTATCTG ATACCGGTTG TCCCCTATCT 
AACATTATCC CCAAGTTTAA TGAATTGTTA TTCAAGAACC AATGGAAGTT GGCACTGGAC 
AAATTGCTAG AGACAAACAA TTTCCCAGAA TTCACTGGAA GAGTATGTCC AGCACCCTGT 
GAGGGAGCTT GTACACTAGG TATTATTGAA GACCCAGTCG GCATAAAATC GGTTGAAAGA 
ATTATCATTG ACAATGCTTT CAAGGAAGGA TGGATTAAGC CTTGTCCACC AAGTACACGC 
ACTGGCTTTA CAGTGGGTGT CATTGGTTCT GGTCCAGCAG GTTTAGCGTG TGCTGATATG 
TTGAACCGTG CCGGACATAC GGTCACTGTT TATGAAAGAT CCGACCGTTG TGGTGGGTTA 
TTGATGTATG GTATTCCAAA CATGAAGTTG GATAAGGCTA TAGTGCAACG TCGTATTGAT 
CTATTGAGTG CCGAAGGTAT TGACTTTGTT ACCAACACCG AAATTGGTAA AACCATAAGC 
ATGGATGAGC TAAAGAACAA GCACAATGCA GTAGTGTATG CTATCGGTTC TACCATTCCA 
CGTGACTTAC CTATTAAGGG TCGTGAATTG AAGAATATTG ATTTTGCCAT GCAGTTGTTG 
GAATCTAACA CAAAAGCTTT ATTGAACAAA GATCTGGAAA TCATTCGTGA AAAGATCCAA 
GGTAAGAAAG TAATTGTTGT CGGTGGTGGT GACACAGGTA ACGATTGTTT AGGTACATCT 
GTAAGACACG GTGCAGCATC AGTTTTGAAT TTCGAATTGT TGCCTGAGCC ACCAGTGGAA 
CGTGCCAAAG ACAATCCATG GCCTCAATGG CCGCGTGTCA TGAGAGTGGA CTACGGTCAT 
GCTGAAGTGA AAGAGCATTA TGGTAGAGAC CCTCGTGAAT ACTGCATCTT GTCCAAGGAA 
TTTATCGGTA ACGATGAGGG TGAAGTCACT GCCATCAGAA CTGTGCGCGT AGAATGGAAG 
AAGTCACAAA GTGGCGTATG GCAAATGGTA GAAATTCCCA ACAGTGAAGA GATCTTTGAA 
GCCGATATCA TTTTGTTGTC TATGGGTTTC GTGGGTCCTG AATTGATCAA TGGCAACGAT 
AACGAAGTTA AGAAGACAAG ACGTGGTACG ATTGCCACAC TCGACGACTC CTCATACTCT 
ATTGATGGAG GAAAGACTTT TGCATGTGGT GACTGTAGAA GAGGGCAATC TTTGATTGTC 
TGGGCCATCC AAGAAGGTAG AAAATGTGCT GCCTCTGTCG ATAAGTTCCT AATGGACGGC 
ACTACGTATC TACCAAGTAA TGGTGGTATC GTTCAACGTG ATTACAAACT ATTGAAAGAA 
TTAGCTAGTC AAGTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The nucleotide sequence of Saccharomyces cerevisiae chromosome IV.
Nature387(6632 Suppl)75-8(1997 May)
Jacq C,Alt-M?rbe J,Andre B,Arnold W,Bahr A,Ballesta JP,Bargues M,Baron L,Becker A,Biteau N,Bl?cker H,Blugeon C,Boskovic J,Brandt P,Br?ckner M,Buitrago MJ,Coster F,Delaveau T,del Rey F,Dujon B,Eide LG,Garcia-Cantalejo JM,Goffeau A,Gomez-Peris A,Zaccaria P

Life with 6000 genes.
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)
Goffeau A,Barrell BG,Bussey H,Davis RW,Dujon B,Feldmann H,Galibert F,Hoheisel JD,Jacq C,Johnston M,Louis EJ,Mewes HW,Murakami Y,Philippsen P,Tettelin H,Oliver SG

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GLT1 cDNA ORF clone, Saccharomyces cerevisiae S288C

Gene

Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

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