CDC24 cDNA ORF clone, Saccharomyces cerevisiae S288C

Gene
Clones
gRNAs

Summary

Gene Symbol	CDC24
Entrez Gene ID	851190
Full Name	Rho family guanine nucleotide exchange factor CDC24
Synonyms	CLS4
Gene Type	protein-coding
Organism	Saccharomyces cerevisiae S288C

mRNA and Protein(s)

mRNA	Protein	Name
NM_001178186.1	NP_009359.1	Rho family guanine nucleotide exchange factor CDC24

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Life with 6000 genes.
Goffeau A, Barrell BG, Bussey H, Davis RW, Dujon B, Feldmann H, Galibert F, Hoheisel JD, Jacq C, Johnston M, Louis EJ, Mewes HW, Murakami Y, Philippsen P, Tettelin H, Oliver SG
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)

The nucleotide sequence of chromosome I from Saccharomyces cerevisiae.
Bussey H, Kaback DB, Zhong W, Vo DT, Clark MW, Fortin N, Hall J, Ouellette BF, Keng T, Barton AB
Proceedings of the National Academy of Sciences of the United States of America92(9)3809-13(1995 Apr)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Biochemical analysis confirmed that this mutation disturbs the interaction with Bem1 but not the fold of the protein. The larger dataset obtained by NGS achieved a more complete representation of the bipartite interaction interface of Cdc24.
Title: A Split-Ubiquitin Based Strategy Selecting for Protein Complex-Interfering Mutations.

We propose that the NH2-terminal region unmasks the DH and PB1 domains, leading to the activation of Cdc42 and interaction with Bem1, respectively, to initiate cell polarization
Title: Essential role of the NH2-terminal region of Cdc24 guanine nucleotide exchange factor in its initial polarized localization in Saccharomyces cerevisiae.

These data support roles for Boi2p, Ent2p, Hua1p, Tos2p, and for Cdc24p phosphorylation in targeting Cdc24p to growth sites.
Title: Multiple proteins and phosphorylations regulate Saccharomyces cerevisiae Cdc24p localization.

study presents the tertiary structure of the heterodimer of Bem1 and Cdc24 PB1 domains determined by NMR spectroscopy
Title: NMR structure of the heterodimer of Bem1 and Cdc24 PB1 domains from Saccharomyces cerevisiae.

Cdc24p oligomerization regulates Cdc42p activation via its localization
Title: Oligomerization regulates the localization of Cdc24, the Cdc42 activator in Saccharomyces cerevisiae.

The following CDC24 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the CDC24 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OSi00777	NM_001178186.1 Latest version!	Saccharomyces cerevisiae S288C Rho family guanine nucleotide exchange factor CDC24 (CDC24), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

CDC24 ( NM_001178186.1 ) cDNA ORF clone, Saccharomyces cerevisiae S288C -> NP_009359.1 Saccharomyces cerevisiae S288C Rho family guanine nucleotide exchange factor CDC24 (CDC24), partial mRNA.

Price & Availability↑

CloneID	OSi00777
Clone ID Related Accession (Same CDS sequence)	NM_001178186.1
Accession Version	NM_001178186.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2565bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-31
Organism	Saccharomyces cerevisiae S288C
Product	Rho family guanine nucleotide exchange factor CDC24
Comment	Comment: REVIEWED REFSEQ: This record has been curated by SGD. This record is derived from an annotated genomic sequence (NC_001133). ##Genome-Annotation-Data-START## Annotation Provider :: SGD Annotation Status :: Full Annotation Annotation Version :: R64-2-1 URL :: http://www.yeastgenome.org/ ##Genome-Annotation-Data-END## COMPLETENESS: incomplete on both ends.

ATGGCGATCC AAACCCGTTT TGCCTCGGGC ACATCTTTAT CCGATTTGAA ACCAAAACCA 
AGTGCAACTT CCATCTCCAT ACCCATGCAA AATGTCATGA ACAAGCCTGT CACGGAACAG 
GACTCACTGT TCCATATATG CGCAAACATC CGGAAAAGAC TGGAGGTGTT ACCTCAACTC 
AAACCTTTTT TACAATTGGC CTACCAATCG AGCGAGGTTT TGAGTGAAAG GCAATCTCTT 
TTGCTATCCC AAAAGCAGCA TCAGGAACTG CTCAAGTCCA ATGGCGCTAA CCGGGACAGT 
AGCGACTTGG CACCAACTTT AAGGTCTAGC TCTATCTCCA CAGCTACCAG TCTCATGTCG 
ATGGAAGGTA TATCATACAC GAATTCGAAT CCCTCGGCCA CCCCAAATAT GGAGGACACT 
TTACTGACTT TTAGTATGGG TATTTTGCCC ATTACCATGG ATTGCGACCC TGTGACACAA 
CTATCACAGC TGTTTCAACA AGGTGCGCCC CTCTGTATAC TTTTCAACTC TGTGAAGCCG 
CAATTTAAAT TACCGGTAAT AGCATCTGAC GATTTGAAAG TCTGTAAAAA ATCCATTTAT 
GACTTTATAT TGGGCTGCAA GAAACACTTT GCATTTAACG ATGAGGAGCT TTTCACTATA 
TCCGACGTTT TTGCCAACTC TACTTCCCAG CTGGTCAAAG TGCTAGAAGT AGTAGAAACG 
CTAATGAATT CCAGCCCTAC TATTTTCCCC TCTAAGAGTA AGACACAGCA AATCATGAAC 
GCAGAAAACC AACACCGACA TCAGCCTCAG CAGTCTTCGA AGAAGCATAA CGAGTATGTT 
AAAATTATCA AGGAATTCGT TGCAACGGAA AGAAAATATG TTCACGATTT GGAAATTTTG 
GATAAATATA GACAGCAGTT ATTAGACAGC AATCTAATAA CGTCTGAAGA GTTGTACATG 
TTGTTCCCTA ATTTGGGTGA TGCTATAGAT TTTCAAAGAA GATTTCTAAT ATCCTTGGAA 
ATAAATGCTT TAGTAGAACC TTCCAAGCAA AGAATCGGGG CTCTTTTCAT GCATTCCAAA 
CATTTTTTTA AGTTGTATGA GCCTTGGTCT ATTGGCCAAA ATGCAGCCAT CGAATTTCTC 
TCTTCAACTT TGCACAAGAT GAGGGTTGAT GAATCGCAGC GGTTCATAAT TAACAATAAA 
CTGGAATTGC AATCCTTCCT TTATAAACCC GTGCAAAGGC TTTGTAGATA TCCCCTGTTG 
GTCAAAGAAT TGCTTGCTGA ATCGAGTGAC GATAATAATA CGAAAGAACT TGAAGCTGCT 
TTAGATATTT CTAAAAATAT TGCGAGAAGT ATCAACGAAA ATCAAAGAAG AACAGAAAAT 
CATCAAGTGG TGAAGAAACT TTATGGTAGA GTGGTCAACT GGAAGGGTTA TAGAATTTCC 
AAGTTCGGTG AGTTATTATA TTTCGATAAA GTGTTCATTT CAACAACAAA TAGCTCCTCG 
GAACCTGAAA GAGAATTTGA GGTTTATCTT TTTGAAAAAA TCATCATCCT TTTTTCAGAG 
GTAGTGACTA AGAAATCTGC ATCATCACTA ATCCTTAAGA AGAAATCCTC AACCTCAGCA 
TCAATCTCCG CCTCGAACAT AACGGACAAC AATGGCAGCC CTCACCACAG TTACCATAAG 
AGGCATAGCA ATAGTAGTAG CAGTAATAAT ATCCATTTAT CTTCGTCTTC AGCAGCGGCG 
ATAATACATT CCAGTACCAA TAGTAGTGAC AACAATTCCA ACAATTCATC ATCATCCTCA 
TTATTCAAGC TGTCCGCTAA CGAACCTAAG CTGGATCTAA GAGGTCGAAT TATGATAATG 
AATCTGAATC AAATCATACC GCAAAACAAC CGGTCATTAA ATATAACATG GGAATCCATA 
AAAGAGCAAG GTAATTTCCT TTTGAAATTC AAAAATGAGG AAACAAGAGA TAATTGGTCA 
TCGTGTTTAC AACAGTTGAT TCATGATCTG AAAAATGAGC AGTTTAAGGC AAGACATCAC 
TCTTCAACAT CGACGACTTC ATCGACAGCC AAATCATCTT CAATGATGTC ACCCACCACA 
ACTATGAATA CACCGAATCA TCACAACAGC CGCCAGACAC ACGATAGTAT GGCTTCTTTC 
TCAAGTTCTC ATATGAAAAG GGTTTCGGAT GTCCTGCCTA AACGGAGGAC CACTTCATCA 
AGTTTCGAAA GTGAAATTAA ATCCATTTCA GAAAATTTCA AGAACTCTAT TCCAGAATCT 
TCCATACTCT TCAGGATATC ATATAATAAC AACTCTAATA ATACCTCTAG TAGCGAGATC 
TTCACACTTT TGGTAGAAAA AGTTTGGAAT TTTGACGACT TGATAATGGC GATCAATTCT 
AAAATTTCGA ATACACATAA TAACAACATT TCACCAATCA CCAAGATCAA ATATCAGGAC 
GAAGATGGGG ATTTTGTTGT GTTAGGTAGC GATGAAGATT GGAATGTTGC TAAAGAAATG 
TTGGCGGAAA ACAATGAGAA ATTCTTGAAC ATTCGTCTGT ATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_009359.1
CDS	1..2565
Translation	MAIQTRFASGTSLSDLKPKPSATSISIPMQNVMNKPVTEQDSLFHICANIRKRLEVLPQLKPFLQLAYQSSEVLSERQSLLLSQKQHQELLKSNGANRDSSDLAPTLRSSSISTATSLMSMEGISYTNSNPSATPNMEDTLLTFSMGILPITMDCDPVTQLSQLFQQGAPLCILFNSVKPQFKLPVIASDDLKVCKKSIYDFILGCKKHFAFNDEELFTISDVFANSTSQLVKVLEVVETLMNSSPTIFPSKSKTQQIMNAENQHRHQPQQSSKKHNEYVKIIKEFVATERKYVHDLEILDKYRQQLLDSNLITSEELYMLFPNLGDAIDFQRRFLISLEINALVEPSKQRIGALFMHSKHFFKLYEPWSIGQNAAIEFLSSTLHKMRVDESQRFIINNKLELQSFLYKPVQRLCRYPLLVKELLAESSDDNNTKELEAALDISKNIARSINENQRRTENHQVVKKLYGRVVNWKGYRISKFGELLYFDKVFISTTNSSSEPEREFEVYLFEKIIILFSEVVTKKSASSLILKKKSSTSASISASNITDNNGSPHHSYHKRHSNSSSSNNIHLSSSSAAAIIHSSTNSSDNNSNNSSSSSLFKLSANEPKLDLRGRIMIMNLNQIIPQNNRSLNITWESIKEQGNFLLKFKNEETRDNWSSCLQQLIHDLKNEQFKARHHSSTSTTSSTAKSSSMMSPTTTMNTPNHHNSRQTHDSMASFSSSHMKRVSDVLPKRRTTSSSFESEIKSISENFKNSIPESSILFRISYNNNSNNTSSSEIFTLLVEKVWNFDDLIMAINSKISNTHNNNISPITKIKYQDEDGDFVVLGSDEDWNVAKEMLAENNEKFLNIRLY

Target ORF information:

RefSeq Version	NM_001178186.1
Organism	Saccharomyces cerevisiae S288C
Definition	Saccharomyces cerevisiae S288C Rho family guanine nucleotide exchange factor CDC24 (CDC24), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001178186.1

ORF Insert Sequence:

ATGGCGATCC AAACCCGTTT TGCCTCGGGC ACATCTTTAT CCGATTTGAA ACCAAAACCA 
AGTGCAACTT CCATCTCCAT ACCCATGCAA AATGTCATGA ACAAGCCTGT CACGGAACAG 
GACTCACTGT TCCATATATG CGCAAACATC CGGAAAAGAC TGGAGGTGTT ACCTCAACTC 
AAACCTTTTT TACAATTGGC CTACCAATCG AGCGAGGTTT TGAGTGAAAG GCAATCTCTT 
TTGCTATCCC AAAAGCAGCA TCAGGAACTG CTCAAGTCCA ATGGCGCTAA CCGGGACAGT 
AGCGACTTGG CACCAACTTT AAGGTCTAGC TCTATCTCCA CAGCTACCAG TCTCATGTCG 
ATGGAAGGTA TATCATACAC GAATTCGAAT CCCTCGGCCA CCCCAAATAT GGAGGACACT 
TTACTGACTT TTAGTATGGG TATTTTGCCC ATTACCATGG ATTGCGACCC TGTGACACAA 
CTATCACAGC TGTTTCAACA AGGTGCGCCC CTCTGTATAC TTTTCAACTC TGTGAAGCCG 
CAATTTAAAT TACCGGTAAT AGCATCTGAC GATTTGAAAG TCTGTAAAAA ATCCATTTAT 
GACTTTATAT TGGGCTGCAA GAAACACTTT GCATTTAACG ATGAGGAGCT TTTCACTATA 
TCCGACGTTT TTGCCAACTC TACTTCCCAG CTGGTCAAAG TGCTAGAAGT AGTAGAAACG 
CTAATGAATT CCAGCCCTAC TATTTTCCCC TCTAAGAGTA AGACACAGCA AATCATGAAC 
GCAGAAAACC AACACCGACA TCAGCCTCAG CAGTCTTCGA AGAAGCATAA CGAGTATGTT 
AAAATTATCA AGGAATTCGT TGCAACGGAA AGAAAATATG TTCACGATTT GGAAATTTTG 
GATAAATATA GACAGCAGTT ATTAGACAGC AATCTAATAA CGTCTGAAGA GTTGTACATG 
TTGTTCCCTA ATTTGGGTGA TGCTATAGAT TTTCAAAGAA GATTTCTAAT ATCCTTGGAA 
ATAAATGCTT TAGTAGAACC TTCCAAGCAA AGAATCGGGG CTCTTTTCAT GCATTCCAAA 
CATTTTTTTA AGTTGTATGA GCCTTGGTCT ATTGGCCAAA ATGCAGCCAT CGAATTTCTC 
TCTTCAACTT TGCACAAGAT GAGGGTTGAT GAATCGCAGC GGTTCATAAT TAACAATAAA 
CTGGAATTGC AATCCTTCCT TTATAAACCC GTGCAAAGGC TTTGTAGATA TCCCCTGTTG 
GTCAAAGAAT TGCTTGCTGA ATCGAGTGAC GATAATAATA CGAAAGAACT TGAAGCTGCT 
TTAGATATTT CTAAAAATAT TGCGAGAAGT ATCAACGAAA ATCAAAGAAG AACAGAAAAT 
CATCAAGTGG TGAAGAAACT TTATGGTAGA GTGGTCAACT GGAAGGGTTA TAGAATTTCC 
AAGTTCGGTG AGTTATTATA TTTCGATAAA GTGTTCATTT CAACAACAAA TAGCTCCTCG 
GAACCTGAAA GAGAATTTGA GGTTTATCTT TTTGAAAAAA TCATCATCCT TTTTTCAGAG 
GTAGTGACTA AGAAATCTGC ATCATCACTA ATCCTTAAGA AGAAATCCTC AACCTCAGCA 
TCAATCTCCG CCTCGAACAT AACGGACAAC AATGGCAGCC CTCACCACAG TTACCATAAG 
AGGCATAGCA ATAGTAGTAG CAGTAATAAT ATCCATTTAT CTTCGTCTTC AGCAGCGGCG 
ATAATACATT CCAGTACCAA TAGTAGTGAC AACAATTCCA ACAATTCATC ATCATCCTCA 
TTATTCAAGC TGTCCGCTAA CGAACCTAAG CTGGATCTAA GAGGTCGAAT TATGATAATG 
AATCTGAATC AAATCATACC GCAAAACAAC CGGTCATTAA ATATAACATG GGAATCCATA 
AAAGAGCAAG GTAATTTCCT TTTGAAATTC AAAAATGAGG AAACAAGAGA TAATTGGTCA 
TCGTGTTTAC AACAGTTGAT TCATGATCTG AAAAATGAGC AGTTTAAGGC AAGACATCAC 
TCTTCAACAT CGACGACTTC ATCGACAGCC AAATCATCTT CAATGATGTC ACCCACCACA 
ACTATGAATA CACCGAATCA TCACAACAGC CGCCAGACAC ACGATAGTAT GGCTTCTTTC 
TCAAGTTCTC ATATGAAAAG GGTTTCGGAT GTCCTGCCTA AACGGAGGAC CACTTCATCA 
AGTTTCGAAA GTGAAATTAA ATCCATTTCA GAAAATTTCA AGAACTCTAT TCCAGAATCT 
TCCATACTCT TCAGGATATC ATATAATAAC AACTCTAATA ATACCTCTAG TAGCGAGATC 
TTCACACTTT TGGTAGAAAA AGTTTGGAAT TTTGACGACT TGATAATGGC GATCAATTCT 
AAAATTTCGA ATACACATAA TAACAACATT TCACCAATCA CCAAGATCAA ATATCAGGAC 
GAAGATGGGG ATTTTGTTGT GTTAGGTAGC GATGAAGATT GGAATGTTGC TAAAGAAATG 
TTGGCGGAAA ACAATGAGAA ATTCTTGAAC ATTCGTCTGT ATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Life with 6000 genes.
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)
Goffeau A,Barrell BG,Bussey H,Davis RW,Dujon B,Feldmann H,Galibert F,Hoheisel JD,Jacq C,Johnston M,Louis EJ,Mewes HW,Murakami Y,Philippsen P,Tettelin H,Oliver SG

The nucleotide sequence of chromosome I from Saccharomyces cerevisiae.
Proceedings of the National Academy of Sciences of the United States of America92(9)3809-13(1995 Apr)
Bussey H,Kaback DB,Zhong W,Vo DT,Clark MW,Fortin N,Hall J,Ouellette BF,Keng T,Barton AB

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CDC24 cDNA ORF clone, Saccharomyces cerevisiae S288C

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gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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