REA1 cDNA ORF clone, Saccharomyces cerevisiae S288C

Gene
Clones
gRNAs

Summary

Gene Symbol	REA1
Entrez Gene ID	850796
Full Name	AAA family ATPase midasin
Synonyms	REA1
Gene Type	protein-coding
Organism	Saccharomyces cerevisiae S288C

mRNA and Protein(s)

mRNA	Protein	Name
NM_001181993.1	NP_013207.1	AAA family ATPase midasin

Pathways from BioSystems

Homologies

Eremothecium gossypii	AGOS_AGR074C	NP_986739.2
Kluyveromyces lactis	KLLA0F19888g	XP_455973.1
Magnaporthe oryzae (rice blast fungus)	MGG_06379	XP_003717195.1
Saccharomyces cerevisiae (baker's yeast)	MDN1	NP_013207.1
Schizosaccharomyces pombe (fission yeast)	SPCC737.08	NP_588370.1
Neurospora crassa	NCU06468	XP_957359.2

Gene Ontology
Bibliography

Related articles in PubMed

The nucleotide sequence of Saccharomyces cerevisiae chromosome XII.
Johnston M, Hillier L, Riles L, Albermann K, Andr? B, Ansorge W, Benes V, Br?ckner M, Delius H, Dubois E, D?sterh?ft A, Entian KD, Floeth M, Goffeau A, Hebling U, Heumann K, Heuss-Neitzel D, Hilbert H, Hilger F, Kleine K, K?tter P, Louis EJ, Messenguy F, Mewes HW, Hoheisel JD
Nature387(6632 Suppl)87-90(1997 May)

Life with 6000 genes.
Goffeau A, Barrell BG, Bussey H, Davis RW, Dujon B, Feldmann H, Galibert F, Hoheisel JD, Jacq C, Johnston M, Louis EJ, Mewes HW, Murakami Y, Philippsen P, Tettelin H, Oliver SG
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Release of Nug2 from pre-60S particles requires both its K(+)-dependent GTPase activity and the remodelling ATPase activity of Rea1.
Title: Coupled GTPase and remodelling ATPase activities form a checkpoint for ribosome export.

Data suggest that Rea1 functions at successive maturation steps to remove ribosomal factors at critical transition points, first driving the exit of early pre-60S particles from the nucleolus and then driving late pre-60S particles from the nucleus.
Title: The AAA-ATPase Rea1 drives removal of biogenesis factors during multiple stages of 60S ribosome assembly.

Rea1 aligns with its interacting partners on the preribosome to effect a necessary step on the path to the export-competent 60S subunit.
Title: Mechanochemical removal of ribosome biogenesis factors from nascent 60S ribosomal subunits.

Rea1 is a dynein-related nuclear AAA-ATPase is involved in late rRNA processing and nuclear export of 60 S subunits
Title: Rea1, a dynein-related nuclear AAA-ATPase, is involved in late rRNA processing and nuclear export of 60 S subunits.

The following REA1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the REA1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OSi00456	NM_001181993.1 Latest version!	Saccharomyces cerevisiae S288C AAA family ATPase midasin (REA1), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

REA1 ( NM_001181993.1 ) cDNA ORF clone, Saccharomyces cerevisiae S288C -> NP_013207.1 Saccharomyces cerevisiae S288C AAA family ATPase midasin (REA1), partial mRNA.

Price & Availability↑

CloneID	OSi00456
Clone ID Related Accession (Same CDS sequence)	NM_001181993.1
Accession Version	NM_001181993.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 14733bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-31
Organism	Saccharomyces cerevisiae S288C
Product	AAA family ATPase midasin
Comment	Comment: REVIEWED REFSEQ: This record has been curated by SGD. This record is derived from an annotated genomic sequence (NC_001144). ##Genome-Annotation-Data-START## Annotation Provider :: SGD Annotation Status :: Full Annotation Annotation Version :: R64-2-1 URL :: http://www.yeastgenome.org/ ##Genome-Annotation-Data-END## COMPLETENESS: incomplete on both ends.

ATGTCCCAGG ATAGAATTTT GTTAGATTTA GACGTAGTTA ACCAAAGGTT AATTCTCTTC 
AATTCTGCTT TTCCCTCTGA TGCTATTGAA GCGCCCTTCC ACTTTAGCAA CAAGGAATCA 
ACTTCTGAGA ATTTAGACAA TTTAGCAGGA ACTATACTAC ATAGCCGTTC CATCACTGGT 
CATGTTTTCC TTTACAAACA TATTTTTTTA GAGATAGTGG CACGTTGGAT AAAGGACTCC 
AAAAAAAAAG ATTACGTTCT TGTAATTGAA AAGTTGGCAA GTATAATTAC AATATTTCCC 
GTTGCAATGC CATTAATTGA AGACTATTTA GATAAAGAGA ATGATCATTT CATCACTATT 
CTTCAAAATC CATCCACACA AAAGGATTCC GACATGTTTA AAATACTATT AGCATACTAT 
AGGTTACTAT ATCACAATAA AGAAGTATTT GCACGCTTCA TTCAACCCGA CATTTTATAC 
CAACTTGTGG ATTTACTCAC CAAAGAACAA GAAAATCAGG TTGTTATATT TCTAGCATTA 
AAGGTTTTAT CTTTATACTT AGACATGGGA GAAAAAACAT TAAACGATAT GCTCGACACT 
TATATAAAAT CACGCGATTC ATTGTTGGGC CATTTTGAGG GCGATTCTGG AATTGATTAC 
AGCTTCCTCG AACTGAACGA AGCTAAGAGA TGTGCAAATT TCTCTAAACT ACCCAGTGTT 
CCAGAATGTT TCACTATAGA AAAAAAATCT AGCTACTTCA TTATTGAGCC TCAGGATTTG 
AGTACTAAGG TCGCGTCAAT CTGTGGCGTT ATCGTCCCTA AGGTCCACAC TATCCACGAC 
AAAGTATTCT ACCCATTAAC CTTTGTCCCT ACTCATAAAA CGGTTTCCTC TCTTCGTCAA 
CTCGGGAGAA AGATTCAAAA TTCAACTCCA ATAATGCTCA TTGGAAAAGC GGGATCCGGT 
AAAACCTTTT TAATCAATGA GCTAAGTAAA TATATGGGTT GCCATGACTC CATTGTGAAA 
ATTCATTTAG GTGAACAAAC TGATGCCAAG TTACTGATTG GTACTTATAC CTCTGGTGAC 
AAGCCAGGCA CGTTTGAATG GAGAGCTGGT GTTCTCGCTA CCGCGGTCAA AGAAGGGAGA 
TGGGTACTTA TAGAAGACAT AGATAAAGCT CCTACAGATG TTTTATCAAT ACTTTTATCG 
CTTTTAGAAA AGCGCGAACT AACCATTCCA TCCAGAGGAG AGACTGTAAA GGCTGCAAAT 
GGTTTCCAGT TAATATCAAC TGTTAGAATA AACGAAGATC ATCAGAAGGA TAGTTCCAAT 
AAGATCTATA ACTTGAATAT GATTGGTATG AGAATCTGGA ATGTTATTGA ATTAGAGGAA 
CCTAGCGAAG AGGATTTGAC ACACATTTTA GCCCAAAAGT TTCCCATTTT GACCAACCTT 
ATCCCCAAAC TAATCGATTC ATACAAAAAC GTCAAAAGTA TTTATATGAA CACCAAGTTC 
ATTTCATTGA ACAAGGGGGC ACACACAAGA GTTGTATCTG TAAGAGATTT AATTAAGCTG 
TGCGAAAGGT TAGACATCCT ATTCAAAAAC AATGGCATCA ACAAACCTGA CCAACTAATC 
CAGTCTTCTG TCTACGACAG TATTTTCTCC GAAGCCGCCG ACTGTTTCGC TGGCGCTATT 
GGAGAATTCA AAGCTTTGGA ACCTATTATA CAAGCAATCG GCGAGTCATT AGACATTGCT 
TCCTCAAGGA TTTCGTTATT TTTGACCCAA CATGTGCCCA CTTTGGAAAA CCTTGATGAT 
AGCATTAAAA TTGGAAGAGC CGTATTACTT AAGGAAAAGC TAAATATACA AAAAAAATCA 
ATGAACAGCA CATTGTTCGC ATTTACGAAT CATTCTTTAA GACTAATGGA ACAGATTTCT 
GTTTGTATAC AGATGACTGA ACCTGTTTTA CTAGTCGGTG AAACGGGTAC CGGGAAAACT 
ACAGTCGTTC AACAACTTGC TAAGATGTTA GCAAAAAAAT TAACGGTAAT CAATGTTTCA 
CAACAAACAG AAACTGGTGA TCTTCTTGGT GGGTACAAAC CCGTAAATTC TAAAACAGTT 
GCAGTTCCTA TACAAGAAAA TTTTGAAACT CTTTTTAATG CTACATTTTC CTTAAAGAAG 
AACGAGAAGT TTCACAAGAT GCTACATAGG TGCTTCAACA AGAATCAATG GAAGAACGTT 
GTTAAACTTT GGAACGAAGC GTACAAAATG GCTCAAAGCA TATTAAAAAT TACAAACACT 
GAAAATGAAA ACGAAAATGC AAAGAAGAAG AAAAGAAGAC TCAATACTCA TGAAAAAAAA 
TTGTTACTTG ACAAATGGGC TGACTTCAAT GACTCAGTGA AGAAGTTTGA GGCCCAGTCT 
AGCTCTATTG AAAACTCGTT TGTCTTCAAT TTTGTCGAGG GTTCTTTGGT AAAGACTATC 
AGGGCTGGGG AGTGGCTATT GCTCGATGAA GTGAATTTAG CAACCGCAGA TACCTTAGAA 
AGTATTTCTG ACCTTCTCAC CGAGCCTGAT TCTCGTAGTA TTCTTCTTTC AGAAAAGGGT 
GACGCTGAGC CTATAAAAGC TCACCCCGAT TTTAGAATAT TTGCTTGTAT GAACCCCGCT 
ACTGATGTTG GTAAAAGAGA CTTACCTATG GGTATCAGAT CAAGATTTAC CGAAATATAT 
GTACATTCTC CGGAACGTGA TATTACTGAT TTGCTTTCCA TAATTGATAA ATATATTGGA 
AAGTACAGCG TCAGCGATGA ATGGGTAGGA AATGACATCG CTGAACTTTA CCTTGAAGCC 
AAAAAACTCT CCGATAACAA TACTATAGTT GATGGCTCAA ACCAAAAACC ACACTTTAGT 
ATCCGTACCC TGACGCGTAC ATTGCTTTAT GTTACTGATA TTATTCATAT CTACGGTCTA 
CGCCGTTCCC TGTATGATGG ATTCTGTATG AGTTTTTTGA CTTTACTGGA CCAAAAATCT 
GAGGCCATTC TGAAGCCAGT AATAGAAAAA TTCACATTGG GGAGGCTAAA AAATGTCAAG 
TCAATCATGT CACAGACACC TCCCTCTCCA GGACCAGATT ATGTTCAGTT TAAGCATTAT 
TGGATGAAAA AGGGCCCTAA TACAATTCAA GAACAAGCTC ACTATATCAT TACGCCTTTT 
GTTGAAAAGA ATATGATGAA TTTGGTAAGA GCTACATCAG GCAAAAGATT TCCTGTGCTT 
ATTCAAGGTC CAACTTCATC GGGAAAAACC AGTATGATCA AATATCTTGC GGATATAACG 
GGCCATAAGT TTGTCCGTAT AAATAACCAT GAGCATACAG ATTTACAGGA ATATTTAGGT 
ACATATGTTA CCGATGATAC CGGAAAGCTG TCGTTTAAGG AAGGTGTTTT AGTCGAGGCA 
CTACGAAAAG GATATTGGAT TGTTTTGGAT GAATTAAATC TTGCCCCAAC CGATGTTTTA 
GAAGCCTTAA ATAGGTTACT TGACGATAAC AGAGAACTTT TTATTCCAGA AACACAAGAA 
GTTGTACATC CTCATCCTGA TTTCCTTCTT TTTGCTACCC AGAATCCACC TGGGATATAC 
GGTGGCCGTA AAATTCTTTC AAGAGCTTTT AGGAATCGTT TTCTGGAGTT GCACTTCGAC 
GATATTCCGC AAGATGAATT AGAAATTATC CTACGTGAGC GCTGCCAAAT TGCCCCATCA 
TATGCAAAGA AAATCGTTGA AGTATACCGC CAATTATCCA TCGAACGTTC CGCTTCAAGG 
TTGTTCGAAC AGAAAAATAG TTTTGCAACT CTACGTGATC TTTTCCGCTG GGCTTTAAGG 
GACGCAGTGG GATATGAGCA GCTTGCTGCC AGTGGCTATA TGCTTCTTGC AGAAAGGTGC 
AGAACTCCAC AAGAAAAAGT TACAGTCAAA AAGACTTTAG AAAAAGTAAT GAAAGTTAAA 
TTAGATATGG ACCAATATTA TGCAAGTCTG GAAGATAAAT CCTTAGAAGC CATTGGCTCT 
GTTACTTGGA CAAAAGGAAT GCGCCGACTA TCCGTCTTAG TTTCTAGTTG TTTAAAGAAT 
AAGGAGCCAG TACTACTGGT AGGTGAAACT GGGTGCGGCA AAACAACAAT TTGCCAATTA 
TTGGCGCAAT TCATGGGTCG AGAACTGATC ACACTAAATG CCCATCAAAA CACAGAAACT 
GGAGACATAT TAGGTGCTCA AAGACCTGTT CGTAACAGGT CTGAAATTCA ATATAAGCTA 
ATAAAATCCT TAAAAACAGC ATTGAATATT GCTAATGACC AAGATGTAGA CTTGAAAGAG 
TTATTACAGC TATATTCTAA GTCAGATAAT AAAAATATAG CCGAAGATGT TCAACTGGAA 
ATACAAAAGC TGAGGGATAG TTTGAATGTT TTATTTGAAT GGAGTGACGG GCCTCTGATT 
CAAGCTATGA GGACGGGAAA TTTTTTTCTA CTTGATGAAA TATCGCTAGC AGATGATTCC 
GTTCTTGAAA GACTAAATAG TGTTTTGGAG CCAGAAAGGA GTTTGTTGTT AGCAGAACAG 
GGTTCATCCG ATAGCCTTGT AACGGCTTCA GAAAACTTTC AATTCTTCGC AACCATGAAC 
CCTGGTGGAG ATTACGGTAA AAAAGAATTA TCTCCAGCGT TAAGAAATAG ATTTACTGAA 
ATATGGGTAC CATCCATGGA AGATTTCAAC GATGTAAACA TGATCGTATC CTCAAGGCTT 
TTAGAGGACT TGAAAGATCT TGCTAATCCA ATTGTGAAAT TCTCTGAATG GTTTGGCAAG 
AAGCTGGGAG GCGGAAATGC AACAAGTGGT GTCATTTCTT TACGTGACAT ACTTGCATGG 
GTTGAATTTA TTAATAAAGT GTTTCCGAAA ATACAGAACA AGTCAACTGC CTTAATTCAA 
GGTGCCTCAA TGGTTTTTAT TGACGCCCTT GGTACAAATA ACACTGCTTA CTTAGCTGAA 
AACGAAAATG ATTTAAAGTC ACTTAGAACA GAATGTATTA TACAACTATT GAAACTATGT 
GGTGACGATT TGGAACTACA ACAAATTGAA ACGAACGAAA TTATTGTGAC TCAGGATGAA 
TTACAAGTCG GAATGTTCAA AATACCAAGA TTTCCAGATG CTCAGTCTTC ATCCTTTAAC 
TTAACCGCCC CCACTACTGC CTCAAACTTA GTAAGGGTTG TACGGGCTAT GCAAGTACAC 
AAGCCAATTT TACTTGAAGG TAGTCCAGGT GTTGGTAAAA CAAGTTTAAT CACCGCTCTT 
GCCAATATTA CAGGAAATAA GCTTACAAGA ATCAATTTGT CTGAACAAAC GGATCTAGTA 
GATCTTTTCG GTGCAGACGC TCCTGGTGAG CGAAGTGGTG AATTTTTATG GCACGATGCT 
CCGTTTTTAA GAGCAATGAA GAAAGGTGAG TGGGTTTTAT TAGATGAAAT GAATTTAGCC 
TCCCAATCCG TCCTAGAAGG GTTGAATGCC TGTTTAGATC ATCGAGGCGA AGCGTATATC 
CCCGAGTTGG ACATATCGTT TTCCTGCCAC CCCAATTTTC TGGTCTTTGC TGCTCAAAAT 
CCTCAGTATC AAGGTGGTGG TAGGAAAGGT CTTCCTAAAT CTTTCGTTAA TCGATTTAGC 
GTGGTATTTA TTGACATGCT TACATCTGAT GATCTCCTTT TGATTGCAAA ACACTTATAT 
CCAAGTATTG AGCCAGATAT CATCGCGAAA ATGATTAAGT TAATGTCTAC TTTAGAGGAT 
CAAGTCTGTA AAAGAAAACT TTGGGGTAAT TCTGGTTCAC CATGGGAGTT CAATTTGAGA 
GATACATTGC GTTGGCTAAA GTTACTCAAC CAATATTCGA TTTGCGAAGA TGTGGACGTG 
TTTGACTTTG TTGATATAAT CGTTAAGCAG AGGTTCCGTA CTATCAGCGA TAAGAATAAA 
GCACAGCTTC TCATTGAAGA TATTTTTGGT AAATTCTCAA CTAAAGAGAA CTTTTTCAAA 
CTTACAGAAG ACTATGTTCA AATTAACAAC GAGGTAGCTC TAAGAAATCC CCATTACCGC 
TATCCAATTA CCCAGAATCT TTTCCCATTA GAGTGCAATG TCGCCGTTTA TGAATCTGTT 
TTGAAAGCAA TCAACAATAA TTGGCCGTTG GTACTTGTTG GGCCCTCAAA CTCCGGTAAA 
ACGGAAACGA TAAGATTTTT AGCTTCAATC TTGGGTCCCA GAGTAGACGT GTTTTCCATG 
AATAGTGATA TAGATAGTAT GGATATCCTT GGTGGTTATG AACAAGTTGA TTTGACTAGA 
CAAATATCAT ATATCACTGA GGAGTTAACA AATATCGTAA GAGAAATAAT CTCGATGAAT 
ATGAAGCTCT CACCAAATGC TACCGCAATC ATGGAAGGTT TGAACTTATT GAAATATTTG 
CTCAATAATA TCGTCACACC TGAGAAATTC CAAGATTTCA GAAATCGTTT TAATAGGTTC 
TTTTCGCACT TAGAAGGGCA TCCCCTATTA AAAACTATGA GTATGAATAT TGAAAAAATG 
ACTGAAATCA TCACTAAGGA AGCTTCGGTT AAATTTGAAT GGTTTGATGG CATGTTGGTC 
AAAGCAGTTG AAAAGGGCCA TTGGCTAATC TTAGATAATG CTAATCTTTG TTCTCCTTCT 
GTTCTTGATA GACTGAACTC TCTATTGGAG ATAGACGGGT CGCTGCTCAT CAATGAATGT 
AGCCAAGAAG ATGGCCAACC AAGGGTTCTC AAACCCCACC CTAACTTCAG ATTATTTTTG 
ACCATGGATC CAAAATATGG TGAATTATCC CGAGCTATGA GAAACAGAGG TGTGGAGATT 
TATATCGACG AATTGCACAG TCGTTCTACA GCCTTCGATC GCCTCACCCT AGGCTTTGAA 
CTTGGAGAGA ATATAGACTT TGTTTCTATT GATGATGGCA TCAAAAAAAT AAAACTAAAT 
GAACCTGATA TGTCGATACC ATTAAAACAC TATGTTCCAT CCTATTTAAG CAGACCATGC 
ATATTTGCAC AAGTACATGA TATTTTATTA TTATCTGACG AAGAACCAAT TGAAGAATCT 
CTTGCTGCTG TTATTCCGAT TTCCCATTTA GGGGAAGTTG GTAAATGGGC CAATAATGTA 
TTGAATTGCA CGGAGTACTC AGAAAAAAAG ATCGCAGAAA GGCTTTATGT TTTTATAACA 
TTTTTGACTG ATATGGGTGT CTTAGAAAAG ATTAATAACC TATACAAACC TGCAAACCTA 
AAATTCCAGA AGGCTTTGGG GCTGCATGAT AAGCAATTGA CAGAGGAAAC TGTCTCGCTT 
ACCTTGAACG AATATGTTCT TCCAACAGTC AGCAAGTATT CAGATAAGAT CAAATCTCCA 
GAGTCGTTAT ACTTATTATC AAGTTTACGT CTGCTACTCA ACTCTTTGAA CGCTCTCAAA 
TTAATCAATG AAAAATCAAC TCATGGTAAA ATTGACGAAC TGACGTATAT TGAATTATCT 
GCGGCTGCCT TTAACGGTCG CCATTTGAAG AATATTCCTC GCATTCCAAT ATTCTGTATT 
CTCTACAATA TTTTAACAGT AATGTCTGAA AACCTTAAGA CGGAAAGTTT ATTTTGCGGC 
TCCAACCAAT ATCAATATTA TTGGGATTTA CTGGTAATTG TAATTGCAGC ATTAGAAACC 
GCCGTTACAA AGGATGAAGC ACGTCTAAGA GTATATAAGG AGTTGATAGA TAGCTGGATT 
GCGTCCGTAA AATCCAAATC AGACATTGAG ATAACTCCCT TCCTTAACAT CAATTTGGAA 
TTTACCGATG TTTTACAGTT GTCGAGGGGC CACTCCATAA CTTTACTATG GGATATATTC 
AGAAAAAATT ATCCGACAAC CTCCAACAGC TGGCTAGCTT TCGAAAAGCT CATTAATTTA 
TCTGAAAAAT TTGACAAAGT ACGTCTGCTC CAATTTTCTG AATCATACAA TTCCATTAAG 
GATTTGATGG ACGTTTTCCG GTTGCTAAAT GATGATGTGT TGAATAATAA ATTAAGTGAG 
TTTAATCTCT TGCTATCAAA GTTAGAAGAT GGTATAAATG AATTGGAACT AATTTCAAAT 
AAGTTTTTAA ACAAAAGAAA ACATTATTTT GCTGACGAAT TTGACAATTT GATCAGATAT 
ACCTTCTCAG TTGACACAGC CGAGCTAATA AAAGAACTTG CTCCCGCTTC TTCTTTAGCC 
ACGCAAAAAC TTACAAAACT TATTACCAAC AAATACAATT ATCCGCCTAT CTTTGATGTT 
CTTTGGACAG AAAAAAATGC TAAATTGACT TCCTTTACTT CTACCATATT TTCATCCCAG 
TTTCTCGAAG ATGTCGTTCG TAAATCTAAT AATTTGAAAT CATTTTCGGG AAATCAGATC 
AAACAATCTA TTAGTGACGC AGAATTGCTA CTTTCCTCCA CTATCAAGTG TTCACCTAAC 
CTACTTAAGA GCCAAATGGA GTACTATAAA AATATGTTAC TGTCATGGCT CAGAAAAGTT 
ATTGATATTC ACGTCGGCGG GGACTGCTTG AAATTGACTT TGAAAGAACT TTGCTCTCTC 
ATAGAAGAAA AAACTGCTTC AGAGACACGC GTGACTTTTG CAGAATACAT CTTTCCCGCT 
CTAGATTTGG CTGAAAGTTC GAAATCATTA GAAGAACTGG GAGAAGCTTG GATCACATTT 
GGTACAGGTT TGCTTCTGCT ATTCGTCCCA GATTCTCCCT ATGATCCGGC AATACACGAT 
TACGTTTTAT ATGATTTATT TTTGAAGACA AAGACATTTT CTCAAAACCT AATGAAAAGT 
TGGAGAAATG TTCGTAAAGT AATCAGTGGC GATGAGGAGA TTTTCACAGA AAAACTTATC 
AATACCATAA GCGATGACGA CGCACCTCAA TCCCCTAGGG TATATCGTAC AGGCATGTCT 
ATTGACTCTT TATTTGATGA ATGGATGGCA TTTTTGTCGT CAACTATGAG TTCTCGTCAA 
ATAAAAGAGC TAGTGAGTTC CTACAAGTGC AATTCAGATC AATCGGATCG TAGATTAGAA 
ATGCTCCAAC AAAATAGTGC ACATTTTTTG AATCGTCTCG AAAGCGGTTA TTCAAAATTT 
GCTGATTTAA ATGATATCCT CGCGGGATAT ATTTACAGTA TCAACTTTGG CTTTGATTTA 
TTGAAGCTTC AAAAATCTAA GGATAGAGCA TCATTCCAAA TTTCTCCTTT ATGGAGTATG 
GACCCTATCA ATATTTCATG CGCAGAGAAT GTTTTAAGCG CTTATCATGA GCTGTCAAGG 
TTCTTCAAAA AGGGTGATAT GGAAGATACT TCGATTGAAA AGGTTTTGAT GTACTTCTTG 
ACGCTGTTTA AGTTCCATAA GAGGGATACA AATTTACTGG AAATTTTTGA GGCTGCTTTG 
TATACTCTGT ATTCAAGATG GTCTGTGAGA CGTTTCAGAC AGGAGCAGGA GGAAAACGAA 
AAGTCAAATA TGTTCAAATT CAATGATAAT TCAGATGATT ACGAGGCAGA CTTTAGAAAG 
CTGTTTCCAG ATTATGAGGA CACAGCTTTG GTAACTAATG AAAAAGATAT TTCGAGTCCT 
GAAAATCTTG ATGACATCTA TTTCAAGCTT GCCGACACAT ATATCTCGGT ATTTGACAAA 
GATCATGACG CCAACTTTTC TTCTGAGTTG AAGAGCGGTG CGATAATAAC AACAATTTTG 
AGTGAAGATT TAAAAAACAC CAGGATAGAA GAGTTGAAAA GCGGATCGTT GTCTGCTGTG 
ATTAATACAC TTGATGCAGA AACACAATCT TTCAAAAACA CGGAAGTTTT TGGTAATATC 
GATTTTTACC ACGATTTCTC TATTCCGGAG TTTCAAAAAG CAGGTGATAT CATTGAAACT 
GTGCTTAAGT CAGTCTTGAA ATTGTTGAAG CAGTGGCCAG AACATGCCAC CCTCAAAGAA 
TTATATCGAG TTTCCCAAGA ATTTTTAAAC TACCCCATTA AAACACCCTT AGCAAGACAG 
CTTCAAAAGA TCGAACAAAT ATATACATAC CTAGCTGAAT GGGAAAAATA CGCTTCCTCG 
GAAGTATCCC TGAATAACAC CGTTAAACTG ATTACAGATT TGATAGTTTC ATGGAGAAAA 
CTAGAATTAC GCACCTGGAA AGGTCTTTTC AACTCGGAAG ACGCAAAAAC CAGAAAATCT 
ATTGGAAAAT GGTGGTTTTA TTTGTACGAG TCAATTGTCA TTTCAAATTT TGTTAGTGAA 
AAGAAGGAAA CGGCCCCTAA CGCTACACTT TTGGTAAGCT CACTTAACTT ATTTTTTAGT 
AAGAGTACAT TGGGCGAATT CAACGCCAGG CTAGATCTTG TAAAAGCATT CTACAAGCAC 
ATTCAACTAA TTGGATTGAG GAGCTCGAAG ATAGCGGGCT TGCTCCATAA TACTATAAAA 
TTCTATTATC AGTTCAAACC ATTAATTGAT GAGCGGATTA CGAATGGAAA AAAGAGTCTG 
GAAAAAGAAA TTGACGATAT AATTCTTTTA GCCAGTTGGA AAGATGTTAA CGTTGATGCC 
CTGAAACAAA GTTCTCGTAA GTCTCATAAC AACCTTTACA AAATTGTCAG AAAATACCGT 
GATTTGCTAA ATGGGGATGC GAAAACCATT ATTGAGGCCG GTCTTTTGTA TTCCAACGAA 
AATAAACTAA AACTCCCTAC TTTAAAACAA CATTTTTACG AAGACCCCAA CTTAGAGGCT 
TCGAAAAATT TGGTGAAAGA AATTTCTACT TGGAGTATGA GAGCTGCGCC ATTAAGGAAT 
ATTGACACGG TAGCAAGTAA CATGGACTCC TACTTGGAAA AGATATCATC ACAGGAATTC 
CCTAACTTCG CAGATTTAGC ATCGGACTTT TATGCTGAAG CTGAAAGGTT ACGTAAGGAA 
ACCCCTAATG TATATACAAA GGAGAACAAA AAGAGGTTGG CATACTTGAA GACTCAAAAA 
AGTAAACTTT TAGGTGATGC ATTGAAAGAA TTGAGAAGAA TCGGTTTAAA AGTCAATTTT 
AGGGAAGACA TTCAAAAAGT TCAATCTTCT ACAACAACTA TTTTGGCAAA CATTGCCCCT 
TTCAATAATG AATATTTAAA TTCGTCAGAC GCATTCTTTT TCAAAATCCT TGACCTTTTA 
CCCAAGCTGA GAAGCGCTGC ATCGAATCCA AGTGATGATA TCCCTGTTGC TGCTATAGAA 
AGAGGTATGG CACTCGCACA GAGTTTGATG TTTTCCCTGA TTACAGTTCG TCACCCTCTA 
TCAGAGTTCA CGAATGATTA CTGTAAGATT AATGGTATGA TGTTAGACCT AGAACACTTT 
ACTTGCTTAA AGGGCGATAT TGTTCATTCT TCATTAAAAG CGAATGTAGA TAATGTCAGA 
TTGTTTGAAA AGTGGCTGCC TTCTTTGTTA GACTATGCCG CACAGACCTT GTCTGTTATT 
TCGAAATACT CAGCAACTTC AGAACAACAA AAGATCTTGT TAGATGCAAA ATCGACCTTA 
TCTTCGTTTT TCGTACACTT CAATTCCAGT CGAATTTTTG ATTCTTCATT TATCGAGTCA 
TATTCTAGAT TTGAGTTATT CATTAATGAA TTATTGAAGA AGCTTGAAAA TGCAAAGGAG 
ACAGGGAACG CTTTCGTTTT TGATATCATT ATAGAGTGGA TCAAAGCAAA TAAAGGGGGT 
CCTATTAAAA AGGAACAGAA GAGAGGCCCT TCGGTGGAAG ATGTTGAACA GGCTTTCCGC 
CGTACTTTCA CATCTATTAT ATTATCTTTC CAGAAGGTAA TTGGAGATGG AATTGAATCT 
ATATCCGAAA CTGATGATAA CTGGTTATCA GCTAGCTTCA AGAAAGTTAT GGTAAACGTT 
AAACTATTAC GATCCAGTGT TGTTTCTAAA AATATAGAGA CAGCATTATC CTTGTTGAAG 
GACTTTGATT TTACCACTAC TGAATCAATT TACGTTAAAT CAGTTATATC CTTTACACTA 
CCGGTAATCA CACGTTATTA CAACGCAATG ACCGTGGTTC TTGAAAGATC TAGAATCTAC 
TACACGAACA CAAGTCGTGG TATGTACATT CTATCGACGA TATTGCATAG CTTAGCCAAA 
AATGGGTTCT GTTCTCCGCA GCCACCATCT GAAGAAGTTG ACGACAAAAA CTTGCAAGAG 
GGTACAGGCT TGGGAGACGG TGAAGGTGCT CAAAATAACA ATAAAGACGT CGAACAAGAC 
GAAGATTTGA CTGAGGATGC ACAAAACGAA AACAAAGAAC AACAAGATAA AGATGAGAGG 
GATGACGAGA ATGAAGATGA TGCAGTTGAG ATGGAGGGCG ATATGGCTGG TGAATTGGAA 
GATTTGTCTA ATGGTGAAGA AAATGATGAT GAAGATACTG ATAGCGAAGA AGAAGAATTA 
GATGAAGAAA TTGATGACCT GAATGAAGAT GACCCCAACG CTATTGATGA TAAAATGTGG 
GATGATAAGG CAAGTGATAA TTCAAAAGAA AAAGATACAG ACCAGAATTT AGATGGAAAA 
AATCAAGAGG AAGATGTACA AGCTGCTGAA AATGACGAAC AACAGCGGGA TAACAAGGAA 
GGAGGGGATG AGGACCCAAA TGCACCTGAA GATGGCGATG AGGAAATTGA AAACGATGAA 
AATGCAGAAG AGGAAAACGA CGTTGGTGAA CAGGAGGACG AAGTTAAAGA TGAAGAAGGC 
GAGGATTTGG AGGCTAATGT TCCTGAAATC GAAACCCTAG ACCTTCCTGA AGATATGAAT 
TTAGATTCAG AGCATGAAGA ATCAGACGAA GATGTTGATA TGTCAGATGG AATGCCTGAT 
GACCTAAACA AGGAAGAGGT TGGAAACGAA GACGAAGAAG TCAAACAAGA ATCCGGTATC 
GAAAGTGATA ATGAAAACGA CGAACCTGGT CCTGAGGAAG ATGCCGGCGA GACAGAAACA 
GCTCTTGACG AAGAAGAAGG TGCTGAAGAG GATGTGGATA TGACTAATGA TGAGGGAAAG 
GAAGACGAAG AAAATGGCCC AGAAGAGCAA GCCATGTCTG ATGAAGAAGA GTTAAAGCAA 
GATGCTGCTA TGGAAGAAAA CAAAGAGAAA GGAGGTGAGC AGAATACAGA AGGTCTTGAT 
GGCGTTGAGG AAAAGGCTGA TACTGAAGAC ATCGATCAAG AAGCTGCCGT TCAACAAGAT 
TCTGGATCCA AAGGTGCTGG TGCTGATGCT ACGGACACAC AAGAACAGGA CGATGTTGGA 
GGTTCAGGAA CGACTCAGAA TACCTATGAA GAAGATCAAG AAGATGTGAC AAAGAATAAT 
GAAGAATCAC GCGAAGAGGC TACGGCTGCT TTAAAGCAAC TAGGTGATAG CATGAAAGAA 
TATCATAGAC GTCGTCAAGA TATAAAAGAA GCACAAACTA ATGGCGAAGA GGACGAAAAT 
TTGGAAAAAA ACAATGAACG TCCCGATGAA TTTGAACATG TTGAGGGTGC AAATACTGAA 
ACAGATACGC AAGCTTTAGG TTCTGCAACA CAGGATCAAT TACAAACCAT TGATGAAGAC 
ATGGCCATTG ATGATGATAG GGAGGAGCAA GAAGTGGACC AGAAAGAGTT AGTAGAAGAC 
GCCGATGATG AAAAAATGGA TATTGATGAA GAAGAGATGC TATCTGATAT CGACGCGCAT 
GACGCTAACA ATGACGTTGA CAGCAAAAAG TCAGGATTCA TCGGTAAAAG AAAGAGTGAG 
GAAGATTTTG AGAACGAACT TTCAAATGAA CACTTCTCGG CTGATCAAGA GGATGACAGT 
GAAATACAAT CTTTAATTGA AAACATTGAG GATAATCCAC CTGATGCAAG TGCTAGTTTG 
ACACCGGAAA GATCGTTAGA AGAATCCCGT GAATTGTGGC ATAAAAGTGA AATTTCGACC 
GCCGATTTAG TATCACGTCT TGGAGAACAA TTAAGACTAA TCTTGGAACC TACCTTGGCT 
ACAAAGCTGA AGGGTGATTA CAAAACAGGT AAGAGACTGA ATATGAAACG GATCATTCCA 
TATATTGCCT CACAATTCCG TAAAGATAAA ATCTGGTTGA GAAGAACTAA ACCAAGCAAA 
CGTCAGTATC AAATAATGAT TGCGCTTGAT GATTCTAAGT CTATGAGCGA ATCTAAATGC 
GTTAAACTCG CTTTTGATAG TTTATGCTTG GTCTCCAAGA CGTTAACTCA GCTAGAGGCT 
GGAGGGTTAT CTATTGTCAA ATTCGGTGAA AATATTAAAG AAGTTCACTC ATTTGACCAA 
CAATTTAGTA ACGAATCTGG CGCAAGAGCT TTTCAATGGT TTGGTTTCCA AGAAACTAAA 
ACAGACGTTA AGAAACTTGT GGCTGAATCT ACTAAAATTT TTGAACGTGC TCGCGCTATG 
GTACATAATG ACCAATGGCA ACTAGAAATT GTAATCTCTG ATGGTATTTG CGAAGACCAT 
GAAACAATAC AAAAACTGGT CCGCCGTGCT AGAGAAAACA AAATTATGCT GGTTTTTGTC 
ATCATAGATG GTATTACTTC TAATGAATCC ATCTTAGACA TGAGCCAAGT GAACTACATT 
CCAGATCAGT ATGGCAATCC GCAATTAAAG ATTACCAAAT ACTTGGACAC ATTTCCTTTT 
GAATTTTATG TTGTTGTACA CGATATAAGC GAACTACCCG AAATGCTTTC ACTGATTTTG 
CGTCAATACT TTACAGACCT GGCATCCAGC TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_013207.1
CDS	1..14733
Translation	MSQDRILLDLDVVNQRLILFNSAFPSDAIEAPFHFSNKESTSENLDNLAGTILHSRSITGHVFLYKHIFLEIVARWIKDSKKKDYVLVIEKLASIITIFPVAMPLIEDYLDKENDHFITILQNPSTQKDSDMFKILLAYYRLLYHNKEVFARFIQPDILYQLVDLLTKEQENQVVIFLALKVLSLYLDMGEKTLNDMLDTYIKSRDSLLGHFEGDSGIDYSFLELNEAKRCANFSKLPSVPECFTIEKKSSYFIIEPQDLSTKVASICGVIVPKVHTIHDKVFYPLTFVPTHKTVSSLRQLGRKIQNSTPIMLIGKAGSGKTFLINELSKYMGCHDSIVKIHLGEQTDAKLLIGTYTSGDKPGTFEWRAGVLATAVKEGRWVLIEDIDKAPTDVLSILLSLLEKRELTIPSRGETVKAANGFQLISTVRINEDHQKDSSNKIYNLNMIGMRIWNVIELEEPSEEDLTHILAQKFPILTNLIPKLIDSYKNVKSIYMNTKFISLNKGAHTRVVSVRDLIKLCERLDILFKNNGINKPDQLIQSSVYDSIFSEAADCFAGAIGEFKALEPIIQAIGESLDIASSRISLFLTQHVPTLENLDDSIKIGRAVLLKEKLNIQKKSMNSTLFAFTNHSLRLMEQISVCIQMTEPVLLVGETGTGKTTVVQQLAKMLAKKLTVINVSQQTETGDLLGGYKPVNSKTVAVPIQENFETLFNATFSLKKNEKFHKMLHRCFNKNQWKNVVKLWNEAYKMAQSILKITNTENENENAKKKKRRLNTHEKKLLLDKWADFNDSVKKFEAQSSSIENSFVFNFVEGSLVKTIRAGEWLLLDEVNLATADTLESISDLLTEPDSRSILLSEKGDAEPIKAHPDFRIFACMNPATDVGKRDLPMGIRSRFTEIYVHSPERDITDLLSIIDKYIGKYSVSDEWVGNDIAELYLEAKKLSDNNTIVDGSNQKPHFSIRTLTRTLLYVTDIIHIYGLRRSLYDGFCMSFLTLLDQKSEAILKPVIEKFTLGRLKNVKSIMSQTPPSPGPDYVQFKHYWMKKGPNTIQEQAHYIITPFVEKNMMNLVRATSGKRFPVLIQGPTSSGKTSMIKYLADITGHKFVRINNHEHTDLQEYLGTYVTDDTGKLSFKEGVLVEALRKGYWIVLDELNLAPTDVLEALNRLLDDNRELFIPETQEVVHPHPDFLLFATQNPPGIYGGRKILSRAFRNRFLELHFDDIPQDELEIILRERCQIAPSYAKKIVEVYRQLSIERSASRLFEQKNSFATLRDLFRWALRDAVGYEQLAASGYMLLAERCRTPQEKVTVKKTLEKVMKVKLDMDQYYASLEDKSLEAIGSVTWTKGMRRLSVLVSSCLKNKEPVLLVGETGCGKTTICQLLAQFMGRELITLNAHQNTETGDILGAQRPVRNRSEIQYKLIKSLKTALNIANDQDVDLKELLQLYSKSDNKNIAEDVQLEIQKLRDSLNVLFEWSDGPLIQAMRTGNFFLLDEISLADDSVLERLNSVLEPERSLLLAEQGSSDSLVTASENFQFFATMNPGGDYGKKELSPALRNRFTEIWVPSMEDFNDVNMIVSSRLLEDLKDLANPIVKFSEWFGKKLGGGNATSGVISLRDILAWVEFINKVFPKIQNKSTALIQGASMVFIDALGTNNTAYLAENENDLKSLRTECIIQLLKLCGDDLELQQIETNEIIVTQDELQVGMFKIPRFPDAQSSSFNLTAPTTASNLVRVVRAMQVHKPILLEGSPGVGKTSLITALANITGNKLTRINLSEQTDLVDLFGADAPGERSGEFLWHDAPFLRAMKKGEWVLLDEMNLASQSVLEGLNACLDHRGEAYIPELDISFSCHPNFLVFAAQNPQYQGGGRKGLPKSFVNRFSVVFIDMLTSDDLLLIAKHLYPSIEPDIIAKMIKLMSTLEDQVCKRKLWGNSGSPWEFNLRDTLRWLKLLNQYSICEDVDVFDFVDIIVKQRFRTISDKNKAQLLIEDIFGKFSTKENFFKLTEDYVQINNEVALRNPHYRYPITQNLFPLECNVAVYESVLKAINNNWPLVLVGPSNSGKTETIRFLASILGPRVDVFSMNSDIDSMDILGGYEQVDLTRQISYITEELTNIVREIISMNMKLSPNATAIMEGLNLLKYLLNNIVTPEKFQDFRNRFNRFFSHLEGHPLLKTMSMNIEKMTEIITKEASVKFEWFDGMLVKAVEKGHWLILDNANLCSPSVLDRLNSLLEIDGSLLINECSQEDGQPRVLKPHPNFRLFLTMDPKYGELSRAMRNRGVEIYIDELHSRSTAFDRLTLGFELGENIDFVSIDDGIKKIKLNEPDMSIPLKHYVPSYLSRPCIFAQVHDILLLSDEEPIEESLAAVIPISHLGEVGKWANNVLNCTEYSEKKIAERLYVFITFLTDMGVLEKINNLYKPANLKFQKALGLHDKQLTEETVSLTLNEYVLPTVSKYSDKIKSPESLYLLSSLRLLLNSLNALKLINEKSTHGKIDELTYIELSAAAFNGRHLKNIPRIPIFCILYNILTVMSENLKTESLFCGSNQYQYYWDLLVIVIAALETAVTKDEARLRVYKELIDSWIASVKSKSDIEITPFLNINLEFTDVLQLSRGHSITLLWDIFRKNYPTTSNSWLAFEKLINLSEKFDKVRLLQFSESYNSIKDLMDVFRLLNDDVLNNKLSEFNLLLSKLEDGINELELISNKFLNKRKHYFADEFDNLIRYTFSVDTAELIKELAPASSLATQKLTKLITNKYNYPPIFDVLWTEKNAKLTSFTSTIFSSQFLEDVVRKSNNLKSFSGNQIKQSISDAELLLSSTIKCSPNLLKSQMEYYKNMLLSWLRKVIDIHVGGDCLKLTLKELCSLIEEKTASETRVTFAEYIFPALDLAESSKSLEELGEAWITFGTGLLLLFVPDSPYDPAIHDYVLYDLFLKTKTFSQNLMKSWRNVRKVISGDEEIFTEKLINTISDDDAPQSPRVYRTGMSIDSLFDEWMAFLSSTMSSRQIKELVSSYKCNSDQSDRRLEMLQQNSAHFLNRLESGYSKFADLNDILAGYIYSINFGFDLLKLQKSKDRASFQISPLWSMDPINISCAENVLSAYHELSRFFKKGDMEDTSIEKVLMYFLTLFKFHKRDTNLLEIFEAALYTLYSRWSVRRFRQEQEENEKSNMFKFNDNSDDYEADFRKLFPDYEDTALVTNEKDISSPENLDDIYFKLADTYISVFDKDHDANFSSELKSGAIITTILSEDLKNTRIEELKSGSLSAVINTLDAETQSFKNTEVFGNIDFYHDFSIPEFQKAGDIIETVLKSVLKLLKQWPEHATLKELYRVSQEFLNYPIKTPLARQLQKIEQIYTYLAEWEKYASSEVSLNNTVKLITDLIVSWRKLELRTWKGLFNSEDAKTRKSIGKWWFYLYESIVISNFVSEKKETAPNATLLVSSLNLFFSKSTLGEFNARLDLVKAFYKHIQLIGLRSSKIAGLLHNTIKFYYQFKPLIDERITNGKKSLEKEIDDIILLASWKDVNVDALKQSSRKSHNNLYKIVRKYRDLLNGDAKTIIEAGLLYSNENKLKLPTLKQHFYEDPNLEASKNLVKEISTWSMRAAPLRNIDTVASNMDSYLEKISSQEFPNFADLASDFYAEAERLRKETPNVYTKENKKRLAYLKTQKSKLLGDALKELRRIGLKVNFREDIQKVQSSTTTILANIAPFNNEYLNSSDAFFFKILDLLPKLRSAASNPSDDIPVAAIERGMALAQSLMFSLITVRHPLSEFTNDYCKINGMMLDLEHFTCLKGDIVHSSLKANVDNVRLFEKWLPSLLDYAAQTLSVISKYSATSEQQKILLDAKSTLSSFFVHFNSSRIFDSSFIESYSRFELFINELLKKLENAKETGNAFVFDIIIEWIKANKGGPIKKEQKRGPSVEDVEQAFRRTFTSIILSFQKVIGDGIESISETDDNWLSASFKKVMVNVKLLRSSVVSKNIETALSLLKDFDFTTTESIYVKSVISFTLPVITRYYNAMTVVLERSRIYYTNTSRGMYILSTILHSLAKNGFCSPQPPSEEVDDKNLQEGTGLGDGEGAQNNNKDVEQDEDLTEDAQNENKEQQDKDERDDENEDDAVEMEGDMAGELEDLSNGEENDDEDTDSEEEELDEEIDDLNEDDPNAIDDKMWDDKASDNSKEKDTDQNLDGKNQEEDVQAAENDEQQRDNKEGGDEDPNAPEDGDEEIENDENAEEENDVGEQEDEVKDEEGEDLEANVPEIETLDLPEDMNLDSEHEESDEDVDMSDGMPDDLNKEEVGNEDEEVKQESGIESDNENDEPGPEEDAGETETALDEEEGAEEDVDMTNDEGKEDEENGPEEQAMSDEEELKQDAAMEENKEKGGEQNTEGLDGVEEKADTEDIDQEAAVQQDSGSKGAGADATDTQEQDDVGGSGTTQNTYEEDQEDVTKNNEESREEATAALKQLGDSMKEYHRRRQDIKEAQTNGEEDENLEKNNERPDEFEHVEGANTETDTQALGSATQDQLQTIDEDMAIDDDREEQEVDQKELVEDADDEKMDIDEEEMLSDIDAHDANNDVDSKKSGFIGKRKSEEDFENELSNEHFSADQEDDSEIQSLIENIEDNPPDASASLTPERSLEESRELWHKSEISTADLVSRLGEQLRLILEPTLATKLKGDYKTGKRLNMKRIIPYIASQFRKDKIWLRRTKPSKRQYQIMIALDDSKSMSESKCVKLAFDSLCLVSKTLTQLEAGGLSIVKFGENIKEVHSFDQQFSNESGARAFQWFGFQETKTDVKKLVAESTKIFERARAMVHNDQWQLEIVISDGICEDHETIQKLVRRARENKIMLVFVIIDGITSNESILDMSQVNYIPDQYGNPQLKITKYLDTFPFEFYVVVHDISELPEMLSLILRQYFTDLASS

Target ORF information:

RefSeq Version	NM_001181993.1
Organism	Saccharomyces cerevisiae S288C
Definition	Saccharomyces cerevisiae S288C AAA family ATPase midasin (REA1), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001181993.1

ORF Insert Sequence:

ATGTCCCAGG ATAGAATTTT GTTAGATTTA GACGTAGTTA ACCAAAGGTT AATTCTCTTC 
AATTCTGCTT TTCCCTCTGA TGCTATTGAA GCGCCCTTCC ACTTTAGCAA CAAGGAATCA 
ACTTCTGAGA ATTTAGACAA TTTAGCAGGA ACTATACTAC ATAGCCGTTC CATCACTGGT 
CATGTTTTCC TTTACAAACA TATTTTTTTA GAGATAGTGG CACGTTGGAT AAAGGACTCC 
AAAAAAAAAG ATTACGTTCT TGTAATTGAA AAGTTGGCAA GTATAATTAC AATATTTCCC 
GTTGCAATGC CATTAATTGA AGACTATTTA GATAAAGAGA ATGATCATTT CATCACTATT 
CTTCAAAATC CATCCACACA AAAGGATTCC GACATGTTTA AAATACTATT AGCATACTAT 
AGGTTACTAT ATCACAATAA AGAAGTATTT GCACGCTTCA TTCAACCCGA CATTTTATAC 
CAACTTGTGG ATTTACTCAC CAAAGAACAA GAAAATCAGG TTGTTATATT TCTAGCATTA 
AAGGTTTTAT CTTTATACTT AGACATGGGA GAAAAAACAT TAAACGATAT GCTCGACACT 
TATATAAAAT CACGCGATTC ATTGTTGGGC CATTTTGAGG GCGATTCTGG AATTGATTAC 
AGCTTCCTCG AACTGAACGA AGCTAAGAGA TGTGCAAATT TCTCTAAACT ACCCAGTGTT 
CCAGAATGTT TCACTATAGA AAAAAAATCT AGCTACTTCA TTATTGAGCC TCAGGATTTG 
AGTACTAAGG TCGCGTCAAT CTGTGGCGTT ATCGTCCCTA AGGTCCACAC TATCCACGAC 
AAAGTATTCT ACCCATTAAC CTTTGTCCCT ACTCATAAAA CGGTTTCCTC TCTTCGTCAA 
CTCGGGAGAA AGATTCAAAA TTCAACTCCA ATAATGCTCA TTGGAAAAGC GGGATCCGGT 
AAAACCTTTT TAATCAATGA GCTAAGTAAA TATATGGGTT GCCATGACTC CATTGTGAAA 
ATTCATTTAG GTGAACAAAC TGATGCCAAG TTACTGATTG GTACTTATAC CTCTGGTGAC 
AAGCCAGGCA CGTTTGAATG GAGAGCTGGT GTTCTCGCTA CCGCGGTCAA AGAAGGGAGA 
TGGGTACTTA TAGAAGACAT AGATAAAGCT CCTACAGATG TTTTATCAAT ACTTTTATCG 
CTTTTAGAAA AGCGCGAACT AACCATTCCA TCCAGAGGAG AGACTGTAAA GGCTGCAAAT 
GGTTTCCAGT TAATATCAAC TGTTAGAATA AACGAAGATC ATCAGAAGGA TAGTTCCAAT 
AAGATCTATA ACTTGAATAT GATTGGTATG AGAATCTGGA ATGTTATTGA ATTAGAGGAA 
CCTAGCGAAG AGGATTTGAC ACACATTTTA GCCCAAAAGT TTCCCATTTT GACCAACCTT 
ATCCCCAAAC TAATCGATTC ATACAAAAAC GTCAAAAGTA TTTATATGAA CACCAAGTTC 
ATTTCATTGA ACAAGGGGGC ACACACAAGA GTTGTATCTG TAAGAGATTT AATTAAGCTG 
TGCGAAAGGT TAGACATCCT ATTCAAAAAC AATGGCATCA ACAAACCTGA CCAACTAATC 
CAGTCTTCTG TCTACGACAG TATTTTCTCC GAAGCCGCCG ACTGTTTCGC TGGCGCTATT 
GGAGAATTCA AAGCTTTGGA ACCTATTATA CAAGCAATCG GCGAGTCATT AGACATTGCT 
TCCTCAAGGA TTTCGTTATT TTTGACCCAA CATGTGCCCA CTTTGGAAAA CCTTGATGAT 
AGCATTAAAA TTGGAAGAGC CGTATTACTT AAGGAAAAGC TAAATATACA AAAAAAATCA 
ATGAACAGCA CATTGTTCGC ATTTACGAAT CATTCTTTAA GACTAATGGA ACAGATTTCT 
GTTTGTATAC AGATGACTGA ACCTGTTTTA CTAGTCGGTG AAACGGGTAC CGGGAAAACT 
ACAGTCGTTC AACAACTTGC TAAGATGTTA GCAAAAAAAT TAACGGTAAT CAATGTTTCA 
CAACAAACAG AAACTGGTGA TCTTCTTGGT GGGTACAAAC CCGTAAATTC TAAAACAGTT 
GCAGTTCCTA TACAAGAAAA TTTTGAAACT CTTTTTAATG CTACATTTTC CTTAAAGAAG 
AACGAGAAGT TTCACAAGAT GCTACATAGG TGCTTCAACA AGAATCAATG GAAGAACGTT 
GTTAAACTTT GGAACGAAGC GTACAAAATG GCTCAAAGCA TATTAAAAAT TACAAACACT 
GAAAATGAAA ACGAAAATGC AAAGAAGAAG AAAAGAAGAC TCAATACTCA TGAAAAAAAA 
TTGTTACTTG ACAAATGGGC TGACTTCAAT GACTCAGTGA AGAAGTTTGA GGCCCAGTCT 
AGCTCTATTG AAAACTCGTT TGTCTTCAAT TTTGTCGAGG GTTCTTTGGT AAAGACTATC 
AGGGCTGGGG AGTGGCTATT GCTCGATGAA GTGAATTTAG CAACCGCAGA TACCTTAGAA 
AGTATTTCTG ACCTTCTCAC CGAGCCTGAT TCTCGTAGTA TTCTTCTTTC AGAAAAGGGT 
GACGCTGAGC CTATAAAAGC TCACCCCGAT TTTAGAATAT TTGCTTGTAT GAACCCCGCT 
ACTGATGTTG GTAAAAGAGA CTTACCTATG GGTATCAGAT CAAGATTTAC CGAAATATAT 
GTACATTCTC CGGAACGTGA TATTACTGAT TTGCTTTCCA TAATTGATAA ATATATTGGA 
AAGTACAGCG TCAGCGATGA ATGGGTAGGA AATGACATCG CTGAACTTTA CCTTGAAGCC 
AAAAAACTCT CCGATAACAA TACTATAGTT GATGGCTCAA ACCAAAAACC ACACTTTAGT 
ATCCGTACCC TGACGCGTAC ATTGCTTTAT GTTACTGATA TTATTCATAT CTACGGTCTA 
CGCCGTTCCC TGTATGATGG ATTCTGTATG AGTTTTTTGA CTTTACTGGA CCAAAAATCT 
GAGGCCATTC TGAAGCCAGT AATAGAAAAA TTCACATTGG GGAGGCTAAA AAATGTCAAG 
TCAATCATGT CACAGACACC TCCCTCTCCA GGACCAGATT ATGTTCAGTT TAAGCATTAT 
TGGATGAAAA AGGGCCCTAA TACAATTCAA GAACAAGCTC ACTATATCAT TACGCCTTTT 
GTTGAAAAGA ATATGATGAA TTTGGTAAGA GCTACATCAG GCAAAAGATT TCCTGTGCTT 
ATTCAAGGTC CAACTTCATC GGGAAAAACC AGTATGATCA AATATCTTGC GGATATAACG 
GGCCATAAGT TTGTCCGTAT AAATAACCAT GAGCATACAG ATTTACAGGA ATATTTAGGT 
ACATATGTTA CCGATGATAC CGGAAAGCTG TCGTTTAAGG AAGGTGTTTT AGTCGAGGCA 
CTACGAAAAG GATATTGGAT TGTTTTGGAT GAATTAAATC TTGCCCCAAC CGATGTTTTA 
GAAGCCTTAA ATAGGTTACT TGACGATAAC AGAGAACTTT TTATTCCAGA AACACAAGAA 
GTTGTACATC CTCATCCTGA TTTCCTTCTT TTTGCTACCC AGAATCCACC TGGGATATAC 
GGTGGCCGTA AAATTCTTTC AAGAGCTTTT AGGAATCGTT TTCTGGAGTT GCACTTCGAC 
GATATTCCGC AAGATGAATT AGAAATTATC CTACGTGAGC GCTGCCAAAT TGCCCCATCA 
TATGCAAAGA AAATCGTTGA AGTATACCGC CAATTATCCA TCGAACGTTC CGCTTCAAGG 
TTGTTCGAAC AGAAAAATAG TTTTGCAACT CTACGTGATC TTTTCCGCTG GGCTTTAAGG 
GACGCAGTGG GATATGAGCA GCTTGCTGCC AGTGGCTATA TGCTTCTTGC AGAAAGGTGC 
AGAACTCCAC AAGAAAAAGT TACAGTCAAA AAGACTTTAG AAAAAGTAAT GAAAGTTAAA 
TTAGATATGG ACCAATATTA TGCAAGTCTG GAAGATAAAT CCTTAGAAGC CATTGGCTCT 
GTTACTTGGA CAAAAGGAAT GCGCCGACTA TCCGTCTTAG TTTCTAGTTG TTTAAAGAAT 
AAGGAGCCAG TACTACTGGT AGGTGAAACT GGGTGCGGCA AAACAACAAT TTGCCAATTA 
TTGGCGCAAT TCATGGGTCG AGAACTGATC ACACTAAATG CCCATCAAAA CACAGAAACT 
GGAGACATAT TAGGTGCTCA AAGACCTGTT CGTAACAGGT CTGAAATTCA ATATAAGCTA 
ATAAAATCCT TAAAAACAGC ATTGAATATT GCTAATGACC AAGATGTAGA CTTGAAAGAG 
TTATTACAGC TATATTCTAA GTCAGATAAT AAAAATATAG CCGAAGATGT TCAACTGGAA 
ATACAAAAGC TGAGGGATAG TTTGAATGTT TTATTTGAAT GGAGTGACGG GCCTCTGATT 
CAAGCTATGA GGACGGGAAA TTTTTTTCTA CTTGATGAAA TATCGCTAGC AGATGATTCC 
GTTCTTGAAA GACTAAATAG TGTTTTGGAG CCAGAAAGGA GTTTGTTGTT AGCAGAACAG 
GGTTCATCCG ATAGCCTTGT AACGGCTTCA GAAAACTTTC AATTCTTCGC AACCATGAAC 
CCTGGTGGAG ATTACGGTAA AAAAGAATTA TCTCCAGCGT TAAGAAATAG ATTTACTGAA 
ATATGGGTAC CATCCATGGA AGATTTCAAC GATGTAAACA TGATCGTATC CTCAAGGCTT 
TTAGAGGACT TGAAAGATCT TGCTAATCCA ATTGTGAAAT TCTCTGAATG GTTTGGCAAG 
AAGCTGGGAG GCGGAAATGC AACAAGTGGT GTCATTTCTT TACGTGACAT ACTTGCATGG 
GTTGAATTTA TTAATAAAGT GTTTCCGAAA ATACAGAACA AGTCAACTGC CTTAATTCAA 
GGTGCCTCAA TGGTTTTTAT TGACGCCCTT GGTACAAATA ACACTGCTTA CTTAGCTGAA 
AACGAAAATG ATTTAAAGTC ACTTAGAACA GAATGTATTA TACAACTATT GAAACTATGT 
GGTGACGATT TGGAACTACA ACAAATTGAA ACGAACGAAA TTATTGTGAC TCAGGATGAA 
TTACAAGTCG GAATGTTCAA AATACCAAGA TTTCCAGATG CTCAGTCTTC ATCCTTTAAC 
TTAACCGCCC CCACTACTGC CTCAAACTTA GTAAGGGTTG TACGGGCTAT GCAAGTACAC 
AAGCCAATTT TACTTGAAGG TAGTCCAGGT GTTGGTAAAA CAAGTTTAAT CACCGCTCTT 
GCCAATATTA CAGGAAATAA GCTTACAAGA ATCAATTTGT CTGAACAAAC GGATCTAGTA 
GATCTTTTCG GTGCAGACGC TCCTGGTGAG CGAAGTGGTG AATTTTTATG GCACGATGCT 
CCGTTTTTAA GAGCAATGAA GAAAGGTGAG TGGGTTTTAT TAGATGAAAT GAATTTAGCC 
TCCCAATCCG TCCTAGAAGG GTTGAATGCC TGTTTAGATC ATCGAGGCGA AGCGTATATC 
CCCGAGTTGG ACATATCGTT TTCCTGCCAC CCCAATTTTC TGGTCTTTGC TGCTCAAAAT 
CCTCAGTATC AAGGTGGTGG TAGGAAAGGT CTTCCTAAAT CTTTCGTTAA TCGATTTAGC 
GTGGTATTTA TTGACATGCT TACATCTGAT GATCTCCTTT TGATTGCAAA ACACTTATAT 
CCAAGTATTG AGCCAGATAT CATCGCGAAA ATGATTAAGT TAATGTCTAC TTTAGAGGAT 
CAAGTCTGTA AAAGAAAACT TTGGGGTAAT TCTGGTTCAC CATGGGAGTT CAATTTGAGA 
GATACATTGC GTTGGCTAAA GTTACTCAAC CAATATTCGA TTTGCGAAGA TGTGGACGTG 
TTTGACTTTG TTGATATAAT CGTTAAGCAG AGGTTCCGTA CTATCAGCGA TAAGAATAAA 
GCACAGCTTC TCATTGAAGA TATTTTTGGT AAATTCTCAA CTAAAGAGAA CTTTTTCAAA 
CTTACAGAAG ACTATGTTCA AATTAACAAC GAGGTAGCTC TAAGAAATCC CCATTACCGC 
TATCCAATTA CCCAGAATCT TTTCCCATTA GAGTGCAATG TCGCCGTTTA TGAATCTGTT 
TTGAAAGCAA TCAACAATAA TTGGCCGTTG GTACTTGTTG GGCCCTCAAA CTCCGGTAAA 
ACGGAAACGA TAAGATTTTT AGCTTCAATC TTGGGTCCCA GAGTAGACGT GTTTTCCATG 
AATAGTGATA TAGATAGTAT GGATATCCTT GGTGGTTATG AACAAGTTGA TTTGACTAGA 
CAAATATCAT ATATCACTGA GGAGTTAACA AATATCGTAA GAGAAATAAT CTCGATGAAT 
ATGAAGCTCT CACCAAATGC TACCGCAATC ATGGAAGGTT TGAACTTATT GAAATATTTG 
CTCAATAATA TCGTCACACC TGAGAAATTC CAAGATTTCA GAAATCGTTT TAATAGGTTC 
TTTTCGCACT TAGAAGGGCA TCCCCTATTA AAAACTATGA GTATGAATAT TGAAAAAATG 
ACTGAAATCA TCACTAAGGA AGCTTCGGTT AAATTTGAAT GGTTTGATGG CATGTTGGTC 
AAAGCAGTTG AAAAGGGCCA TTGGCTAATC TTAGATAATG CTAATCTTTG TTCTCCTTCT 
GTTCTTGATA GACTGAACTC TCTATTGGAG ATAGACGGGT CGCTGCTCAT CAATGAATGT 
AGCCAAGAAG ATGGCCAACC AAGGGTTCTC AAACCCCACC CTAACTTCAG ATTATTTTTG 
ACCATGGATC CAAAATATGG TGAATTATCC CGAGCTATGA GAAACAGAGG TGTGGAGATT 
TATATCGACG AATTGCACAG TCGTTCTACA GCCTTCGATC GCCTCACCCT AGGCTTTGAA 
CTTGGAGAGA ATATAGACTT TGTTTCTATT GATGATGGCA TCAAAAAAAT AAAACTAAAT 
GAACCTGATA TGTCGATACC ATTAAAACAC TATGTTCCAT CCTATTTAAG CAGACCATGC 
ATATTTGCAC AAGTACATGA TATTTTATTA TTATCTGACG AAGAACCAAT TGAAGAATCT 
CTTGCTGCTG TTATTCCGAT TTCCCATTTA GGGGAAGTTG GTAAATGGGC CAATAATGTA 
TTGAATTGCA CGGAGTACTC AGAAAAAAAG ATCGCAGAAA GGCTTTATGT TTTTATAACA 
TTTTTGACTG ATATGGGTGT CTTAGAAAAG ATTAATAACC TATACAAACC TGCAAACCTA 
AAATTCCAGA AGGCTTTGGG GCTGCATGAT AAGCAATTGA CAGAGGAAAC TGTCTCGCTT 
ACCTTGAACG AATATGTTCT TCCAACAGTC AGCAAGTATT CAGATAAGAT CAAATCTCCA 
GAGTCGTTAT ACTTATTATC AAGTTTACGT CTGCTACTCA ACTCTTTGAA CGCTCTCAAA 
TTAATCAATG AAAAATCAAC TCATGGTAAA ATTGACGAAC TGACGTATAT TGAATTATCT 
GCGGCTGCCT TTAACGGTCG CCATTTGAAG AATATTCCTC GCATTCCAAT ATTCTGTATT 
CTCTACAATA TTTTAACAGT AATGTCTGAA AACCTTAAGA CGGAAAGTTT ATTTTGCGGC 
TCCAACCAAT ATCAATATTA TTGGGATTTA CTGGTAATTG TAATTGCAGC ATTAGAAACC 
GCCGTTACAA AGGATGAAGC ACGTCTAAGA GTATATAAGG AGTTGATAGA TAGCTGGATT 
GCGTCCGTAA AATCCAAATC AGACATTGAG ATAACTCCCT TCCTTAACAT CAATTTGGAA 
TTTACCGATG TTTTACAGTT GTCGAGGGGC CACTCCATAA CTTTACTATG GGATATATTC 
AGAAAAAATT ATCCGACAAC CTCCAACAGC TGGCTAGCTT TCGAAAAGCT CATTAATTTA 
TCTGAAAAAT TTGACAAAGT ACGTCTGCTC CAATTTTCTG AATCATACAA TTCCATTAAG 
GATTTGATGG ACGTTTTCCG GTTGCTAAAT GATGATGTGT TGAATAATAA ATTAAGTGAG 
TTTAATCTCT TGCTATCAAA GTTAGAAGAT GGTATAAATG AATTGGAACT AATTTCAAAT 
AAGTTTTTAA ACAAAAGAAA ACATTATTTT GCTGACGAAT TTGACAATTT GATCAGATAT 
ACCTTCTCAG TTGACACAGC CGAGCTAATA AAAGAACTTG CTCCCGCTTC TTCTTTAGCC 
ACGCAAAAAC TTACAAAACT TATTACCAAC AAATACAATT ATCCGCCTAT CTTTGATGTT 
CTTTGGACAG AAAAAAATGC TAAATTGACT TCCTTTACTT CTACCATATT TTCATCCCAG 
TTTCTCGAAG ATGTCGTTCG TAAATCTAAT AATTTGAAAT CATTTTCGGG AAATCAGATC 
AAACAATCTA TTAGTGACGC AGAATTGCTA CTTTCCTCCA CTATCAAGTG TTCACCTAAC 
CTACTTAAGA GCCAAATGGA GTACTATAAA AATATGTTAC TGTCATGGCT CAGAAAAGTT 
ATTGATATTC ACGTCGGCGG GGACTGCTTG AAATTGACTT TGAAAGAACT TTGCTCTCTC 
ATAGAAGAAA AAACTGCTTC AGAGACACGC GTGACTTTTG CAGAATACAT CTTTCCCGCT 
CTAGATTTGG CTGAAAGTTC GAAATCATTA GAAGAACTGG GAGAAGCTTG GATCACATTT 
GGTACAGGTT TGCTTCTGCT ATTCGTCCCA GATTCTCCCT ATGATCCGGC AATACACGAT 
TACGTTTTAT ATGATTTATT TTTGAAGACA AAGACATTTT CTCAAAACCT AATGAAAAGT 
TGGAGAAATG TTCGTAAAGT AATCAGTGGC GATGAGGAGA TTTTCACAGA AAAACTTATC 
AATACCATAA GCGATGACGA CGCACCTCAA TCCCCTAGGG TATATCGTAC AGGCATGTCT 
ATTGACTCTT TATTTGATGA ATGGATGGCA TTTTTGTCGT CAACTATGAG TTCTCGTCAA 
ATAAAAGAGC TAGTGAGTTC CTACAAGTGC AATTCAGATC AATCGGATCG TAGATTAGAA 
ATGCTCCAAC AAAATAGTGC ACATTTTTTG AATCGTCTCG AAAGCGGTTA TTCAAAATTT 
GCTGATTTAA ATGATATCCT CGCGGGATAT ATTTACAGTA TCAACTTTGG CTTTGATTTA 
TTGAAGCTTC AAAAATCTAA GGATAGAGCA TCATTCCAAA TTTCTCCTTT ATGGAGTATG 
GACCCTATCA ATATTTCATG CGCAGAGAAT GTTTTAAGCG CTTATCATGA GCTGTCAAGG 
TTCTTCAAAA AGGGTGATAT GGAAGATACT TCGATTGAAA AGGTTTTGAT GTACTTCTTG 
ACGCTGTTTA AGTTCCATAA GAGGGATACA AATTTACTGG AAATTTTTGA GGCTGCTTTG 
TATACTCTGT ATTCAAGATG GTCTGTGAGA CGTTTCAGAC AGGAGCAGGA GGAAAACGAA 
AAGTCAAATA TGTTCAAATT CAATGATAAT TCAGATGATT ACGAGGCAGA CTTTAGAAAG 
CTGTTTCCAG ATTATGAGGA CACAGCTTTG GTAACTAATG AAAAAGATAT TTCGAGTCCT 
GAAAATCTTG ATGACATCTA TTTCAAGCTT GCCGACACAT ATATCTCGGT ATTTGACAAA 
GATCATGACG CCAACTTTTC TTCTGAGTTG AAGAGCGGTG CGATAATAAC AACAATTTTG 
AGTGAAGATT TAAAAAACAC CAGGATAGAA GAGTTGAAAA GCGGATCGTT GTCTGCTGTG 
ATTAATACAC TTGATGCAGA AACACAATCT TTCAAAAACA CGGAAGTTTT TGGTAATATC 
GATTTTTACC ACGATTTCTC TATTCCGGAG TTTCAAAAAG CAGGTGATAT CATTGAAACT 
GTGCTTAAGT CAGTCTTGAA ATTGTTGAAG CAGTGGCCAG AACATGCCAC CCTCAAAGAA 
TTATATCGAG TTTCCCAAGA ATTTTTAAAC TACCCCATTA AAACACCCTT AGCAAGACAG 
CTTCAAAAGA TCGAACAAAT ATATACATAC CTAGCTGAAT GGGAAAAATA CGCTTCCTCG 
GAAGTATCCC TGAATAACAC CGTTAAACTG ATTACAGATT TGATAGTTTC ATGGAGAAAA 
CTAGAATTAC GCACCTGGAA AGGTCTTTTC AACTCGGAAG ACGCAAAAAC CAGAAAATCT 
ATTGGAAAAT GGTGGTTTTA TTTGTACGAG TCAATTGTCA TTTCAAATTT TGTTAGTGAA 
AAGAAGGAAA CGGCCCCTAA CGCTACACTT TTGGTAAGCT CACTTAACTT ATTTTTTAGT 
AAGAGTACAT TGGGCGAATT CAACGCCAGG CTAGATCTTG TAAAAGCATT CTACAAGCAC 
ATTCAACTAA TTGGATTGAG GAGCTCGAAG ATAGCGGGCT TGCTCCATAA TACTATAAAA 
TTCTATTATC AGTTCAAACC ATTAATTGAT GAGCGGATTA CGAATGGAAA AAAGAGTCTG 
GAAAAAGAAA TTGACGATAT AATTCTTTTA GCCAGTTGGA AAGATGTTAA CGTTGATGCC 
CTGAAACAAA GTTCTCGTAA GTCTCATAAC AACCTTTACA AAATTGTCAG AAAATACCGT 
GATTTGCTAA ATGGGGATGC GAAAACCATT ATTGAGGCCG GTCTTTTGTA TTCCAACGAA 
AATAAACTAA AACTCCCTAC TTTAAAACAA CATTTTTACG AAGACCCCAA CTTAGAGGCT 
TCGAAAAATT TGGTGAAAGA AATTTCTACT TGGAGTATGA GAGCTGCGCC ATTAAGGAAT 
ATTGACACGG TAGCAAGTAA CATGGACTCC TACTTGGAAA AGATATCATC ACAGGAATTC 
CCTAACTTCG CAGATTTAGC ATCGGACTTT TATGCTGAAG CTGAAAGGTT ACGTAAGGAA 
ACCCCTAATG TATATACAAA GGAGAACAAA AAGAGGTTGG CATACTTGAA GACTCAAAAA 
AGTAAACTTT TAGGTGATGC ATTGAAAGAA TTGAGAAGAA TCGGTTTAAA AGTCAATTTT 
AGGGAAGACA TTCAAAAAGT TCAATCTTCT ACAACAACTA TTTTGGCAAA CATTGCCCCT 
TTCAATAATG AATATTTAAA TTCGTCAGAC GCATTCTTTT TCAAAATCCT TGACCTTTTA 
CCCAAGCTGA GAAGCGCTGC ATCGAATCCA AGTGATGATA TCCCTGTTGC TGCTATAGAA 
AGAGGTATGG CACTCGCACA GAGTTTGATG TTTTCCCTGA TTACAGTTCG TCACCCTCTA 
TCAGAGTTCA CGAATGATTA CTGTAAGATT AATGGTATGA TGTTAGACCT AGAACACTTT 
ACTTGCTTAA AGGGCGATAT TGTTCATTCT TCATTAAAAG CGAATGTAGA TAATGTCAGA 
TTGTTTGAAA AGTGGCTGCC TTCTTTGTTA GACTATGCCG CACAGACCTT GTCTGTTATT 
TCGAAATACT CAGCAACTTC AGAACAACAA AAGATCTTGT TAGATGCAAA ATCGACCTTA 
TCTTCGTTTT TCGTACACTT CAATTCCAGT CGAATTTTTG ATTCTTCATT TATCGAGTCA 
TATTCTAGAT TTGAGTTATT CATTAATGAA TTATTGAAGA AGCTTGAAAA TGCAAAGGAG 
ACAGGGAACG CTTTCGTTTT TGATATCATT ATAGAGTGGA TCAAAGCAAA TAAAGGGGGT 
CCTATTAAAA AGGAACAGAA GAGAGGCCCT TCGGTGGAAG ATGTTGAACA GGCTTTCCGC 
CGTACTTTCA CATCTATTAT ATTATCTTTC CAGAAGGTAA TTGGAGATGG AATTGAATCT 
ATATCCGAAA CTGATGATAA CTGGTTATCA GCTAGCTTCA AGAAAGTTAT GGTAAACGTT 
AAACTATTAC GATCCAGTGT TGTTTCTAAA AATATAGAGA CAGCATTATC CTTGTTGAAG 
GACTTTGATT TTACCACTAC TGAATCAATT TACGTTAAAT CAGTTATATC CTTTACACTA 
CCGGTAATCA CACGTTATTA CAACGCAATG ACCGTGGTTC TTGAAAGATC TAGAATCTAC 
TACACGAACA CAAGTCGTGG TATGTACATT CTATCGACGA TATTGCATAG CTTAGCCAAA 
AATGGGTTCT GTTCTCCGCA GCCACCATCT GAAGAAGTTG ACGACAAAAA CTTGCAAGAG 
GGTACAGGCT TGGGAGACGG TGAAGGTGCT CAAAATAACA ATAAAGACGT CGAACAAGAC 
GAAGATTTGA CTGAGGATGC ACAAAACGAA AACAAAGAAC AACAAGATAA AGATGAGAGG 
GATGACGAGA ATGAAGATGA TGCAGTTGAG ATGGAGGGCG ATATGGCTGG TGAATTGGAA 
GATTTGTCTA ATGGTGAAGA AAATGATGAT GAAGATACTG ATAGCGAAGA AGAAGAATTA 
GATGAAGAAA TTGATGACCT GAATGAAGAT GACCCCAACG CTATTGATGA TAAAATGTGG 
GATGATAAGG CAAGTGATAA TTCAAAAGAA AAAGATACAG ACCAGAATTT AGATGGAAAA 
AATCAAGAGG AAGATGTACA AGCTGCTGAA AATGACGAAC AACAGCGGGA TAACAAGGAA 
GGAGGGGATG AGGACCCAAA TGCACCTGAA GATGGCGATG AGGAAATTGA AAACGATGAA 
AATGCAGAAG AGGAAAACGA CGTTGGTGAA CAGGAGGACG AAGTTAAAGA TGAAGAAGGC 
GAGGATTTGG AGGCTAATGT TCCTGAAATC GAAACCCTAG ACCTTCCTGA AGATATGAAT 
TTAGATTCAG AGCATGAAGA ATCAGACGAA GATGTTGATA TGTCAGATGG AATGCCTGAT 
GACCTAAACA AGGAAGAGGT TGGAAACGAA GACGAAGAAG TCAAACAAGA ATCCGGTATC 
GAAAGTGATA ATGAAAACGA CGAACCTGGT CCTGAGGAAG ATGCCGGCGA GACAGAAACA 
GCTCTTGACG AAGAAGAAGG TGCTGAAGAG GATGTGGATA TGACTAATGA TGAGGGAAAG 
GAAGACGAAG AAAATGGCCC AGAAGAGCAA GCCATGTCTG ATGAAGAAGA GTTAAAGCAA 
GATGCTGCTA TGGAAGAAAA CAAAGAGAAA GGAGGTGAGC AGAATACAGA AGGTCTTGAT 
GGCGTTGAGG AAAAGGCTGA TACTGAAGAC ATCGATCAAG AAGCTGCCGT TCAACAAGAT 
TCTGGATCCA AAGGTGCTGG TGCTGATGCT ACGGACACAC AAGAACAGGA CGATGTTGGA 
GGTTCAGGAA CGACTCAGAA TACCTATGAA GAAGATCAAG AAGATGTGAC AAAGAATAAT 
GAAGAATCAC GCGAAGAGGC TACGGCTGCT TTAAAGCAAC TAGGTGATAG CATGAAAGAA 
TATCATAGAC GTCGTCAAGA TATAAAAGAA GCACAAACTA ATGGCGAAGA GGACGAAAAT 
TTGGAAAAAA ACAATGAACG TCCCGATGAA TTTGAACATG TTGAGGGTGC AAATACTGAA 
ACAGATACGC AAGCTTTAGG TTCTGCAACA CAGGATCAAT TACAAACCAT TGATGAAGAC 
ATGGCCATTG ATGATGATAG GGAGGAGCAA GAAGTGGACC AGAAAGAGTT AGTAGAAGAC 
GCCGATGATG AAAAAATGGA TATTGATGAA GAAGAGATGC TATCTGATAT CGACGCGCAT 
GACGCTAACA ATGACGTTGA CAGCAAAAAG TCAGGATTCA TCGGTAAAAG AAAGAGTGAG 
GAAGATTTTG AGAACGAACT TTCAAATGAA CACTTCTCGG CTGATCAAGA GGATGACAGT 
GAAATACAAT CTTTAATTGA AAACATTGAG GATAATCCAC CTGATGCAAG TGCTAGTTTG 
ACACCGGAAA GATCGTTAGA AGAATCCCGT GAATTGTGGC ATAAAAGTGA AATTTCGACC 
GCCGATTTAG TATCACGTCT TGGAGAACAA TTAAGACTAA TCTTGGAACC TACCTTGGCT 
ACAAAGCTGA AGGGTGATTA CAAAACAGGT AAGAGACTGA ATATGAAACG GATCATTCCA 
TATATTGCCT CACAATTCCG TAAAGATAAA ATCTGGTTGA GAAGAACTAA ACCAAGCAAA 
CGTCAGTATC AAATAATGAT TGCGCTTGAT GATTCTAAGT CTATGAGCGA ATCTAAATGC 
GTTAAACTCG CTTTTGATAG TTTATGCTTG GTCTCCAAGA CGTTAACTCA GCTAGAGGCT 
GGAGGGTTAT CTATTGTCAA ATTCGGTGAA AATATTAAAG AAGTTCACTC ATTTGACCAA 
CAATTTAGTA ACGAATCTGG CGCAAGAGCT TTTCAATGGT TTGGTTTCCA AGAAACTAAA 
ACAGACGTTA AGAAACTTGT GGCTGAATCT ACTAAAATTT TTGAACGTGC TCGCGCTATG 
GTACATAATG ACCAATGGCA ACTAGAAATT GTAATCTCTG ATGGTATTTG CGAAGACCAT 
GAAACAATAC AAAAACTGGT CCGCCGTGCT AGAGAAAACA AAATTATGCT GGTTTTTGTC 
ATCATAGATG GTATTACTTC TAATGAATCC ATCTTAGACA TGAGCCAAGT GAACTACATT 
CCAGATCAGT ATGGCAATCC GCAATTAAAG ATTACCAAAT ACTTGGACAC ATTTCCTTTT 
GAATTTTATG TTGTTGTACA CGATATAAGC GAACTACCCG AAATGCTTTC ACTGATTTTG 
CGTCAATACT TTACAGACCT GGCATCCAGC TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The nucleotide sequence of Saccharomyces cerevisiae chromosome XII.
Nature387(6632 Suppl)87-90(1997 May)
Johnston M,Hillier L,Riles L,Albermann K,Andr? B,Ansorge W,Benes V,Br?ckner M,Delius H,Dubois E,D?sterh?ft A,Entian KD,Floeth M,Goffeau A,Hebling U,Heumann K,Heuss-Neitzel D,Hilbert H,Hilger F,Kleine K,K?tter P,Louis EJ,Messenguy F,Mewes HW,Hoheisel JD

Life with 6000 genes.
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)
Goffeau A,Barrell BG,Bussey H,Davis RW,Dujon B,Feldmann H,Galibert F,Hoheisel JD,Jacq C,Johnston M,Louis EJ,Mewes HW,Murakami Y,Philippsen P,Tettelin H,Oliver SG

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REA1 cDNA ORF clone, Saccharomyces cerevisiae S288C

Gene

Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

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