RPN11 cDNA ORF clone, Saccharomyces cerevisiae S288C

Gene
Clones
gRNAs

Summary

Gene Symbol	RPN11
Entrez Gene ID	850554
Full Name	proteasome regulatory particle lid subunit RPN11
Synonyms	MPR1
Gene Type	protein-coding
Organism	Saccharomyces cerevisiae S288C

mRNA and Protein(s)

mRNA	Protein	Name
NM_001179969.1	NP_116659.1	proteasome regulatory particle lid subunit RPN11

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	PSMD14	XP_857441.2
Saccharomyces cerevisiae (baker's yeast)	RPN11	NP_116659.1
Arabidopsis thaliana (thale cress)	AT5G23540	NP_197745.1
Xenopus tropicalis (tropical clawed frog)	psmd14	NP_001025636.1
Pan troglodytes (chimpanzee)	PSMD14	XP_515855.2
Bos taurus (cattle)	PSMD14	NP_001069535.1
Gallus gallus (chicken)	PSMD14	NP_001026427.2
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP008617	XP_314713.2
Caenorhabditis elegans (roundworm)	rpn-11	NP_494712.1
Eremothecium gossypii	AGOS_AGR006W	NP_986671.1
Magnaporthe oryzae (rice blast fungus)	MGG_16706	XP_003711682.1
Neurospora crassa	NCU00823	XP_964366.2
Oryza sativa (rice)	Os01g0267200	NP_001042683.1
	PSMD14	XP_001096177.1
Rattus norvegicus (Norway rat)	Psmd14	NP_001020860.1
Kluyveromyces lactis	KLLA0E14147g	XP_454588.1
Homo sapiens (human)	PSMD14	NP_005796.1
Drosophila melanogaster (fruit fly)	Rpn11	NP_608905.1
Schizosaccharomyces pombe (fission yeast)	rpn11	NP_594014.1
Mus musculus (house mouse)	Psmd14	NP_067501.2
Danio rerio (zebrafish)	psmd14	NP_001077042.1
Oryza sativa (rice)	Os05g0371200	NP_001055355.1

Gene Ontology
Bibliography

Related articles in PubMed

Life with 6000 genes.
Goffeau A, Barrell BG, Bussey H, Davis RW, Dujon B, Feldmann H, Galibert F, Hoheisel JD, Jacq C, Johnston M, Louis EJ, Mewes HW, Murakami Y, Philippsen P, Tettelin H, Oliver SG
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)

Analysis of the nucleotide sequence of chromosome VI from Saccharomyces cerevisiae.
Murakami Y, Naitou M, Hagiwara H, Shibata T, Ozawa M, Sasanuma S, Sasanuma M, Tsuchiya Y, Soeda E, Yokoyama K
Nature genetics10(3)261-8(1995 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Report the ubiquitin-bound structure of Rpn11 from S. cerevisiae and the mechanisms for mechanochemical coupling of substrate degradation and deubiquitination.
Title: An AAA Motor-Driven Mechanical Switch in Rpn11 Controls Deubiquitination at the 26S Proteasome.

Overall, this work demonstrates that the co-opted Rpn11p, which is involved in the assembly of the functional proteasome, also functions in the proper assembly of the tombusvirus viral replicase complex.
Title: The proteasomal Rpn11 metalloprotease suppresses tombusvirus RNA recombination and promotes viral replication via facilitating assembly of the viral replicase complex.

Fluorescently labeled tetra-Ub chains revealed endo-chain preference for Ubp6 acting on Lys(48) and random action for Rpn11.
Title: Disassembly of Lys11 and mixed linkage polyubiquitin conjugates provides insights into function of proteasomal deubiquitinases Rpn11 and Ubp6.

A key role for rpn11 in 26S proteasome
Title: Base-CP proteasome can serve as a platform for stepwise lid formation.

The Rpn11 active site at the entrance to the ATPase ring pore is likely to prevent erroneous deubiquitylation of folded proteins.
Title: Crystal structure of the proteasomal deubiquitylation module Rpn8-Rpn11.

The following RPN11 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the RPN11 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OSi00240	NM_001179969.1 Latest version!	Saccharomyces cerevisiae S288C proteasome regulatory particle lid subunit RPN11 (RPN11), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$174.30 $249.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

RPN11 ( NM_001179969.1 ) cDNA ORF clone, Saccharomyces cerevisiae S288C -> NP_116659.1 Saccharomyces cerevisiae S288C proteasome regulatory particle lid subunit RPN11 (RPN11), partial mRNA.

Price & Availability↑

CloneID	OSi00240
Clone ID Related Accession (Same CDS sequence)	NM_001179969.1
Accession Version	NM_001179969.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 921bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-31
Organism	Saccharomyces cerevisiae S288C
Product	proteasome regulatory particle lid subunit RPN11
Comment	Comment: REVIEWED REFSEQ: This record has been curated by SGD. This record is derived from an annotated genomic sequence (NC_001138). ##Genome-Annotation-Data-START## Annotation Provider :: SGD Annotation Status :: Full Annotation Annotation Version :: R64-2-1 URL :: http://www.yeastgenome.org/ ##Genome-Annotation-Data-END## COMPLETENESS: incomplete on both ends.

ATGGAACGAC TACAGAGATT GATGATGAAT AGTAAGGTGG GGTCCGCGGA CACCGGCCGT 
GACGATACGA AAGAAACTGT TTATATTTCT TCGATTGCGC TTTTAAAGAT GCTAAAGCAT 
GGTAGAGCTG GTGTTCCCAT GGAAGTCATG GGGTTGATGT TAGGTGAGTT TGTCGATGAT 
TATACGGTTA ACGTTGTGGA CGTGTTTGCG ATGCCTCAAT CGGGTACCGG AGTTTCTGTT 
GAGGCTGTCG ATGATGTTTT CCAAGCGAAG ATGATGGACA TGTTAAAACA AACGGGCAGA 
GACCAAATGG TCGTTGGCTG GTACCACTCT CATCCAGGGT TTGGCTGTTG GCTATCTTCT 
GTTGATGTTA ATACTCAAAA ATCTTTTGAA CAACTAAACA GCAGAGCTGT TGCTGTCGTT 
GTTGACCCTA TTCAATCCGT TAAGGGAAAA GTTGTCATCG ATGCTTTTAG ATTGATTGAC 
ACGGGCGCAT TGATAAATAA CTTAGAGCCT AGACAAACAA CCTCCAACAC AGGCTTATTG 
AACAAGGCCA ACATTCAAGC CTTAATTCAC GGTCTGAATA GGCATTACTA TTCTTTAAAT 
ATTGATTATC ATAAAACCGC GAAGGAAACC AAGATGTTAA TGAACTTACA TAAAGAACAG 
TGGCAATCAG GTCTTAAGAT GTACGATTAT GAAGAAAAAG AAGAATCAAA TTTGGCTGCT 
ACAAAGAGTA TGGTTAAGAT AGCCGAACAG TACTCTAAGA GAATAGAAGA GGAAAAGGAA 
TTAACCGAAG AAGAACTTAA GACAAGATAC GTTGGTAGGC AAGATCCAAA GAAGCACCTT 
TCCGAAACAG CAGATGAGAC ACTAGAGAAC AATATTGTTT CTGTGCTGAC GGCGGGTGTT 
AATTCAGTGG CAATTAAATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_116659.1
CDS	1..921
Translation	MERLQRLMMNSKVGSADTGRDDTKETVYISSIALLKMLKHGRAGVPMEVMGLMLGEFVDDYTVNVVDVFAMPQSGTGVSVEAVDDVFQAKMMDMLKQTGRDQMVVGWYHSHPGFGCWLSSVDVNTQKSFEQLNSRAVAVVVDPIQSVKGKVVIDAFRLIDTGALINNLEPRQTTSNTGLLNKANIQALIHGLNRHYYSLNIDYHKTAKETKMLMNLHKEQWQSGLKMYDYEEKEESNLAATKSMVKIAEQYSKRIEEEKELTEEELKTRYVGRQDPKKHLSETADETLENNIVSVLTAGVNSVAIK

Target ORF information:

RefSeq Version	NM_001179969.1
Organism	Saccharomyces cerevisiae S288C
Definition	Saccharomyces cerevisiae S288C proteasome regulatory particle lid subunit RPN11 (RPN11), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001179969.1

ORF Insert Sequence:

ATGGAACGAC TACAGAGATT GATGATGAAT AGTAAGGTGG GGTCCGCGGA CACCGGCCGT 
GACGATACGA AAGAAACTGT TTATATTTCT TCGATTGCGC TTTTAAAGAT GCTAAAGCAT 
GGTAGAGCTG GTGTTCCCAT GGAAGTCATG GGGTTGATGT TAGGTGAGTT TGTCGATGAT 
TATACGGTTA ACGTTGTGGA CGTGTTTGCG ATGCCTCAAT CGGGTACCGG AGTTTCTGTT 
GAGGCTGTCG ATGATGTTTT CCAAGCGAAG ATGATGGACA TGTTAAAACA AACGGGCAGA 
GACCAAATGG TCGTTGGCTG GTACCACTCT CATCCAGGGT TTGGCTGTTG GCTATCTTCT 
GTTGATGTTA ATACTCAAAA ATCTTTTGAA CAACTAAACA GCAGAGCTGT TGCTGTCGTT 
GTTGACCCTA TTCAATCCGT TAAGGGAAAA GTTGTCATCG ATGCTTTTAG ATTGATTGAC 
ACGGGCGCAT TGATAAATAA CTTAGAGCCT AGACAAACAA CCTCCAACAC AGGCTTATTG 
AACAAGGCCA ACATTCAAGC CTTAATTCAC GGTCTGAATA GGCATTACTA TTCTTTAAAT 
ATTGATTATC ATAAAACCGC GAAGGAAACC AAGATGTTAA TGAACTTACA TAAAGAACAG 
TGGCAATCAG GTCTTAAGAT GTACGATTAT GAAGAAAAAG AAGAATCAAA TTTGGCTGCT 
ACAAAGAGTA TGGTTAAGAT AGCCGAACAG TACTCTAAGA GAATAGAAGA GGAAAAGGAA 
TTAACCGAAG AAGAACTTAA GACAAGATAC GTTGGTAGGC AAGATCCAAA GAAGCACCTT 
TCCGAAACAG CAGATGAGAC ACTAGAGAAC AATATTGTTT CTGTGCTGAC GGCGGGTGTT 
AATTCAGTGG CAATTAAATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Life with 6000 genes.
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)
Goffeau A,Barrell BG,Bussey H,Davis RW,Dujon B,Feldmann H,Galibert F,Hoheisel JD,Jacq C,Johnston M,Louis EJ,Mewes HW,Murakami Y,Philippsen P,Tettelin H,Oliver SG

Analysis of the nucleotide sequence of chromosome VI from Saccharomyces cerevisiae.
Nature genetics10(3)261-8(1995 Jul)
Murakami Y,Naitou M,Hagiwara H,Shibata T,Ozawa M,Sasanuma S,Sasanuma M,Tsuchiya Y,Soeda E,Yokoyama K

見積依頼

Eメール

japanmarket@genscript.com

電話

03-6811-6572

FAX

03-6811-6573
050-3174-7589(受注専用)

オンラインフォーム

オンライン注文

RPN11 cDNA ORF clone, Saccharomyces cerevisiae S288C

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

見積依頼

Eメール

電話

FAX

オンラインフォーム

サポート

会社情報

サポート

会社情報