CDC39 cDNA ORF clone, Saccharomyces cerevisiae S288C

Gene
Clones
gRNAs

Summary

Gene Symbol	CDC39
Entrez Gene ID	850455
Full Name	CCR4-NOT core subunit CDC39
Synonyms	NOT1,ROS1,SMD6
Gene Type	protein-coding
Organism	Saccharomyces cerevisiae S288C

mRNA and Protein(s)

mRNA	Protein	Name
NM_001178799.1	NP_010017.2	CCR4-NOT core subunit CDC39

Pathways from BioSystems

Homologies

Kluyveromyces lactis	KLLA0C18612g	XP_453029.1
Eremothecium gossypii	AGOS_ABR112C	NP_983059.2
Saccharomyces cerevisiae (baker's yeast)	CDC39	NP_010017.2

Gene Ontology
Bibliography

Related articles in PubMed

Life with 6000 genes.
Goffeau A, Barrell BG, Bussey H, Davis RW, Dujon B, Feldmann H, Galibert F, Hoheisel JD, Jacq C, Johnston M, Louis EJ, Mewes HW, Murakami Y, Philippsen P, Tettelin H, Oliver SG
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)

The complete DNA sequence of yeast chromosome III.
Oliver SG, van der Aart QJ, Agostoni-Carbone ML, Aigle M, Alberghina L, Alexandraki D, Antoine G, Anwar R, Ballesta JP, Benit P
Nature357(6373)38-46(1992 May)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Studies indicate that the Ccr4 (carbon catabolite repressed 4)-Not (negative on TATA-less) complex has been connected to every step of the mRNA lifecycle.
Title: Ccr4-Not is at the core of the eukaryotic gene expression circuitry.

The interplay between Ccr4-Not and Rpb4/7 described here suggests a mechanism for how the cell coordinates mRNA synthesis and decay
Title: The Rpb4/7 module of RNA polymerase II is required for carbon catabolite repressor protein 4-negative on TATA (Ccr4-not) complex to promote elongation.

Not2 and Not5 have extended regions that wrap around Not1 and around their globular domains, the Not boxes.
Title: Structure and RNA-binding properties of the Not1-Not2-Not5 module of the yeast Ccr4-Not complex.

A MIF4G domain positioned centrally within the Not1 protein recognizes Caf1, which in turn binds the LRR domain of Ccr4 and tethers the Ccr4 nuclease domain.
Title: Architecture of the nuclease module of the yeast Ccr4-not complex: the Not1-Caf1-Ccr4 interaction.

Results describe the structure of the purified CCR4-NOT complex as determined by electron microscopy.
Title: Insights into the structure of the CCR4-NOT complex by electron microscopy.

The following CDC39 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the CDC39 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OSi00156	NM_001178799.1 Latest version!	Saccharomyces cerevisiae S288C CCR4-NOT core subunit CDC39 (CDC39), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1161.30 $1659.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

CDC39 ( NM_001178799.1 ) cDNA ORF clone, Saccharomyces cerevisiae S288C -> NP_010017.2 Saccharomyces cerevisiae S288C CCR4-NOT core subunit CDC39 (CDC39), partial mRNA.

Price & Availability↑

CloneID	OSi00156
Clone ID Related Accession (Same CDS sequence)	NM_001178799.1
Accession Version	NM_001178799.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6327bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-31
Organism	Saccharomyces cerevisiae S288C
Product	CCR4-NOT core subunit CDC39
Comment	Comment: REVIEWED REFSEQ: This record has been curated by SGD. This record is derived from an annotated genomic sequence (NC_001135). ##Genome-Annotation-Data-START## Annotation Provider :: SGD Annotation Status :: Full Annotation Annotation Version :: R64-2-1 URL :: http://www.yeastgenome.org/ ##Genome-Annotation-Data-END## COMPLETENESS: incomplete on both ends.

ATGCTATCGG CCACATACCG TGATTTGAAC ACAGCATCTA ATTTAGAAAC ATCAAAGGAA 
AAACAGGCCG CTCAAATCGT CATTGCACAA ATTAGTTTAT TATTCACGAC TCTTAACAAC 
GACAATTTTG AATCCGTGGA AAGAGAAATT AGACATATTT TAGACAGGTC GTCCGTAGAT 
ATTTACATAA AAGTTTGGGA ACGATTATTA ACCTTAAGTT CTCGGGATAT TTTACAAGCG 
GGAAAATTTT TACTTCAAGA AAATCTACTA CACAGACTAC TATTAGAATT TGCGAAGGAT 
TTACCGAAGA AAAGCACAGA CCTTATTGAG CTTTTGAAAG AACGAACCTT CAATAACCAG 
GAGTTTCAAA AACAAACAGG AATTACATTA TCACTTTTCA TTGATCTATT TGATAAATCT 
GCAAACAAGG ACATTATAGA GTCACTTGAC CGCTCCTCTC AGATTAACGA TTTCAAGACA 
ATTAAGATGA ATCATACAAA TTATTTAAGG AATTTTTTTC TTCAAACCAC ACCAGAAACA 
CTAGAGTCCA ATCTACGCGA CTTATTGCAT TCCTTGGAAG GTGAAAGTCT AAATGACTTA 
TTAGCTCTTT TACTGTCCGA AATACTTTCA CCTGGGTCTC AGAATTTACA AAATGATCCC 
ACACGGAGTT GGTTGACACC TCCGATGGTT TTAGACGCAA CGAACCGTGG GAACGTTATA 
GCAAGATCTA TAAGTTCTCT GCAAGCCAAC CAGATAAATT GGAATCGTGT GTTTAATTTA 
ATGTCAACAA AGTATTTCTT GAGCGCACCA TTGATGCCTA CTACAGCATC TTTGAGTTGC 
TTATTTGCAG CATTGCACGA TGGTCCAGTT ATTGATGAAT TTTTCAGTTG CGACTGGAAA 
GTTATTTTCA AACTAGATTT GGCCATTCAA CTTCATAAGT GGTCGGTACA GAATGGTTGC 
TTTGACTTAT TAAATGCAGA AGGTACCAGG AAAGTTTCTG AAACCATCCC AAACACAAAG 
CAATCTTTAC TCTACTTATT ATCCATTGCA TCATTGAATT TAGAATTGTT CCTACAAAGG 
GAGGAATTGT CTGATGGTCC TATGCTAGCT TATTTTCAAG AGTGCTTCTT TGAAGATTTC 
AACTACGCCC CTGAATATCT TATTTTAGCA TTAGTCAAAG AAATGAAGCG GTTCGTTTTA 
TTGATAGAAA ACAGGACAGT CATAGACGAA ATACTTATTA CCTTATTGAT TCAAGTGCAT 
AATAAATCAC CGTCATCGTT CAAGGACGTT ATTTCTACAA TAACCGATGA TTCTAAAATC 
GTAGATGCAG CAAAAATCAT AATCAACTCG GATGACGCAC CTATTGCCAA CTTTTTAAAA 
TCGTTGTTAG ATACGGGAAG ATTAGATACG GTCATTAATA AACTTCCTTT CAATGAAGCT 
TTTAAAATTT TGCCATGCGC AAGACAAATT GGTTGGGAGG GGTTCGATAC TTTCTTAAAA 
ACAAAAGTTT CTCCATCTAA TGTCGATGTA GTGCTGGAAT CACTAGAGGT TCAAACGAAA 
ATGACTGATA CAAACACTCC ATTTAGGTCA TTAAAGACAT TTGACTTATT CGCTTTTCAT 
TCATTAATTG AAGTACTGAA CAAATGCCCA CTAGATGTTC TCCAATTACA AAGGTTTGAA 
TCCTTGGAAT TTTCCTTATT AATTGCATTT CCTAGATTGA TCAATTTTGG TTTTGGACAC 
GATGAAGCTA TTTTAGCCAA TGGTGACATC GCAGGGATTA ATAATGATAT TGAAAAGGAG 
ATGCAGAACT ATTTACAGAA AATGTATAGT GGTGAGTTAG CCATTAAAGA TGTAATCGAA 
CTTCTGAGAA GGTTAAGAGA TAGCGACTTG CCAAGGGACC AGGAAGTCTT CACATGTATT 
ACCCATGCCG TTATAGCAGA ATCGACATTC TTCCAAGATT ATCCATTGGA TGCATTGGCT 
ACTACATCTG TTCTTTTTGG ATCCATGATT CTCTTTCAAC TGTTACGTGG ATTCGTATTA 
GACGTCGCAT TTAGGATAAT CATGAGGTTT GCCAAGGAGC CTCCAGAGTC CAAGATGTTT 
AAGTTTGCTG TACAAGCTAT TTATGCATTT AGGATACGTT TGGCCGAATA TCCACAGTAT 
TGTAAGGACC TCTTGAGAGA TGTTCCGGCT TTGAAGTCTC AGGCTCAAGT TTACCAATCT 
ATCGTCGAAG CTGCTACCCT AGCAAATGCT CCAAAGGAAA GGTCAAGACC CGTCCAGGAA 
ATGATCCCAT TAAAATTTTT TGCTGTAGAT GAAGTTTCAT GTCAGATCAA TCAAGAAGGT 
GCTCCTAAAG ATGTCGTAGA AAAAGTTCTT TTTGTTCTCA ACAACGTTAC TCTGGCTAAC 
TTGAATAATA AGGTTGATGA ATTGAAAAAA AGTTTGACAC CAAATTATTT TTCTTGGTTT 
TCCACATATT TAGTTACGCA AAGGGCTAAA ACAGAACCTA ACTATCATGA TCTTTATAGC 
AAGGTTATAG TTGCTATGGG GTCAGGGTTG CTACATCAGT TCATGGTCAA CGTTACTTTG 
AGACAATTAT TTGTCCTACT ATCTACAAAA GACGAGCAAG CCATCGATAA AAAGCACCTA 
AAGAATTTGG CTTCATGGTT AGGATGTATC ACATTAGCTT TGAATAAACC AATTAAACAC 
AAGAATATCG CATTCAGGGA AATGTTAATC GAAGCTTATA AGGAAAATAG ACTTGAAATA 
GTTGTGCCTT TTGTAACAAA GATTTTACAA AGGGCTTCTG AATCAAAAAT TTTCAAGCCT 
CCAAATCCCT GGACTGTTGG CATATTAAAG CTGTTGATTG AGTTGAACGA AAAAGCAAAC 
TGGAAATTAA GTTTGACTTT CGAAGTTGAG GTTTTATTAA AATCTTTTAA TTTGACCACC 
AAATCTCTCA AGCCCTCGAA TTTCATCAAT ACTCCGGAAG TTATAGAAAC TTTATCCGGT 
GCTTTGGGAT CAATCACTCT GGAGCAACAA CAAACAGAGC AACAAAGGCA AATTATACTA 
ATGCAACAAC ACCAGCAACA GATGCTAATA TATCAACAGA GACAACAACA ACAACAACAA 
AGGCAACAAC AACAACAACA TCATATTAGT GCAAATACAA TCGCAGACCA ACAAGCGGCA 
TTTGGCGGCG AGGGTTCAAT TTCACACGAC AATCCTTTTA ACAACTTACT TGGTTCTACT 
ATTTTTGTAA CCCACCCTGA CTTGAAGAGG GTATTTCAAA TGGCTTTAGC CAAGTCAGTT 
CGCGAAATTT TGTTGGAAGT AGTCGAAAAG TCATCAGGAA TTGCTGTTGT TACGACGACA 
AAAATAATAC TTAAAGACTT TGCCACTGAA GTTGATGAGT CTAAGTTGAA GACGGCTGCA 
ATCATTATGG TAAGGCATTT GGCACAAAGT TTAGCTCGAG CTACTTCAAT TGAACCATTG 
AAAGAAGGCA TACGTTCTAC TATGCAATCA CTAGCACCGA ATTTAATGTC TCTTTCTTCT 
TCACCTGCAG AGGAGCTTGA CACGGCAATA AATGAAAATA TTGGCATTGC TCTAGTTTTG 
ATTGAGAAAG CATCTATGGA CAAGTCTACT CAAGATTTAG CAGACCAATT GATGCAAGCG 
ATTGCTATTC GTCGTTATCA CAAGGAAAGA AGGGCAGACC AACCATTTAT TACGCAAAAT 
ACCAATCCAT ATTCACTGTC TTTACCAGAA CCTCTTGGTT TGAAAAACAC TGGTGTTACT 
CCTCAACAAT TCAGGGTATA CGAAGAATTT GGTAAGAATA TTCCAAACTT GGATGTTATT 
CCGTTTGCAG GATTGCCCGC TCACGCTCCA CCGATGACTC AAAATGTGGG TTTAACTCAG 
CCTCAGCAAC AACAAGCGCA AATGCCTACC CAAATCCTAA CCTCCGAACA AATAAGAGCT 
CAACAACAAC AGCAGCAATT ACAGAAAAGC CGTTTGAATC AGCCATCCCA GTCGGCTCAA 
CCTCCAGGAG TGAATGTCCC AAATCCTCAA GGTGGGATTG CTGCAGTTCA ATCAGATTTG 
GAACAGAATC AACGTGTTCT CGTTCACCTC ATGGACATTT TAGTTTCTCA AATTAAAGAA 
AATGCTACGA AGAATAACTT AGCTGAATTA GGCGATCAAA ACCAAATTAA AACCATCATT 
TTTCAAATTT TGACATTCAT TGCAAAAAGC GCACAAAAGG ATCAATTAGC TTTAAAGGTA 
TCCCAAGCTG TCGTTAATAG CCTTTTTGCC ACTAGTGAGA GTCCTCTCTG CAGAGAAGTT 
TTGTCCCTAC TTTTGGAAAA GTTATGTTCT TTATCCCTCG TTGCTAGAAA AGACGTTGTC 
TGGTGGTTAG TTTATGCCTT GGACAGTAGG AAATTCAATG TTCCCGTTAT CAGATCCCTT 
CTAGAAGTTA ATTTAATTGA TGCTACAGAA TTAGATAACG TTTTAGTTAC TGCAATGAAA 
AATAAAATGG AGAACTCAAC TGAATTTGCT ATGAAATTAA TTCAGAATAC TGTCTTGTCT 
GATGATCCAA TTTTGATGAG AATGGACTTC ATTAAAACCT TAGAACACTT GGCCTCTTCG 
GAAGATGAAA ATGTAAAGAA ATTCATCAAA GAGTTCGAAG ATACTAAGAT AATGCCAGTG 
AGGAAAGGTA CCAAAACCAC AAGAACAGAA AAGCTTTACT TAGTATTTAC GGAATGGGTA 
AAATTACTTC AAAGAGTTGA GAATAACGAC GTAATCACAA CTGTTTTTAT CAAGCAATTA 
GTCGAAAAGG GTGTTATCAG CGATACTGAT AATTTACTTA CATTTGTCAA AAGTTCTCTT 
GAGCTATCAG TTTCTTCATT CAAAGAAAGT GACCCGACTG ATGAGGTTTT CATCGCTATT 
GATGCTCTAG GATCGCTAAT TATAAAATTG TTGATTTTAC AGGGTTTCAA AGATGATACA 
AGAAGAGATT ACATAAATGC AATATTTTCT GTGATCGTTT TAGTGTTTGC TAAGGATCAT 
AGCCAAGAGG GTACCACATT CAATGAACGA CCATATTTCA GACTATTTTC TAACATCTTA 
TACGAATGGG CTACCATCAG GACGCACAAT TTTGTTAGAA TATCTGATTC CAGCACTAGG 
CAGGAGCTGA TCGAATTTGA TTCTGTATTT TACAACACTT TCTCAGGATA TTTGCACGCT 
CTGCAACCAT TTGCCTTCCC TGGATTCTCA TTTGCATGGG TGACACTATT ATCACACAGA 
ATGTTATTAC CAATTATGCT AAGATTACCC AATAAAATAG GTTGGGAAAA GTTAATGCTT 
TTGATTATCG ATTTGTTTAA ATTTTTGGAC CAATACACAA GTAAACATGC AGTCTCTGAC 
GCTGTTTCGG TTGTTTATAA GGGAACACTG CGTGTTATTT TAGGCATTTC GAATGATATG 
CCATCCTTTT TGATTGAAAA TCACTATGAA TTAATGAACA ATCTACCTCC AACATATTTC 
CAACTAAAGA ATGTTATTTT ATCTGCTATT CCTAAGAATA TGACCGTTCC CAACCCATAT 
GACGTGGATC TTAATATGGA GGATATTCCA GCATGTAAAG AACTACCTGA AGTCTTCTTT 
GATCCTGTAA TTGATTTACA CTCATTGAAA AAGCCAGTTG ACAACTACCT ACGTATTCCC 
TCAAATTCAT TATTAAGAAC AATACTAAGC GCTATTTACA AGGATACCTA TGACATAAAA 
AAGGGCGTAG GCTACGACTT TTTATCTGTT GATAGTAAAT TAATTCGCGC TATTGTATTA 
CATGTGGGCA TTGAAGCTGG AATAGAGTAT AAGAGAACTT CTTCAAATGC GGTATTTAAT 
ACGAAGTCTT CTTATTATAC TTTATTGTTC AATCTGATTC AAAATGGTAG CATCGAAATG 
AAATATCAAA TTATTCTGTC TATTGTGGAA CAATTGCGGT ATCCAAACAT CCACACCTAT 
TGGTTCAGCT TTGTGTTAAT GAATATGTTC AAAAGTGACG AATGGAATGA TCAAAAACTT 
GAAGTCCAAG AAATTATTTT AAGAAACTTT TTAAAAAGAA TTATTGTTAA CAAACCACAT 
ACCTGGGGTG TTTCAGTTTT CTTTACTCAG TTGATAAACA ATAACGATAT TAATCTTTTA 
GACCTGCCCT TTGTACAAAG TGTTCCCGAA ATTAAACTAA TTTTACAACA ATTAGTAAAA 
TATTCCAAAA AATACACAAC CAGTGAACAA GATGACCAAT CCGCCACCAT CAATAGAAGG 
CAAACCCCTC TACAATCCAA CGCATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_010017.2
CDS	1..6327
Translation	MLSATYRDLNTASNLETSKEKQAAQIVIAQISLLFTTLNNDNFESVEREIRHILDRSSVDIYIKVWERLLTLSSRDILQAGKFLLQENLLHRLLLEFAKDLPKKSTDLIELLKERTFNNQEFQKQTGITLSLFIDLFDKSANKDIIESLDRSSQINDFKTIKMNHTNYLRNFFLQTTPETLESNLRDLLHSLEGESLNDLLALLLSEILSPGSQNLQNDPTRSWLTPPMVLDATNRGNVIARSISSLQANQINWNRVFNLMSTKYFLSAPLMPTTASLSCLFAALHDGPVIDEFFSCDWKVIFKLDLAIQLHKWSVQNGCFDLLNAEGTRKVSETIPNTKQSLLYLLSIASLNLELFLQREELSDGPMLAYFQECFFEDFNYAPEYLILALVKEMKRFVLLIENRTVIDEILITLLIQVHNKSPSSFKDVISTITDDSKIVDAAKIIINSDDAPIANFLKSLLDTGRLDTVINKLPFNEAFKILPCARQIGWEGFDTFLKTKVSPSNVDVVLESLEVQTKMTDTNTPFRSLKTFDLFAFHSLIEVLNKCPLDVLQLQRFESLEFSLLIAFPRLINFGFGHDEAILANGDIAGINNDIEKEMQNYLQKMYSGELAIKDVIELLRRLRDSDLPRDQEVFTCITHAVIAESTFFQDYPLDALATTSVLFGSMILFQLLRGFVLDVAFRIIMRFAKEPPESKMFKFAVQAIYAFRIRLAEYPQYCKDLLRDVPALKSQAQVYQSIVEAATLANAPKERSRPVQEMIPLKFFAVDEVSCQINQEGAPKDVVEKVLFVLNNVTLANLNNKVDELKKSLTPNYFSWFSTYLVTQRAKTEPNYHDLYSKVIVAMGSGLLHQFMVNVTLRQLFVLLSTKDEQAIDKKHLKNLASWLGCITLALNKPIKHKNIAFREMLIEAYKENRLEIVVPFVTKILQRASESKIFKPPNPWTVGILKLLIELNEKANWKLSLTFEVEVLLKSFNLTTKSLKPSNFINTPEVIETLSGALGSITLEQQQTEQQRQIILMQQHQQQMLIYQQRQQQQQQRQQQQQHHISANTIADQQAAFGGEGSISHDNPFNNLLGSTIFVTHPDLKRVFQMALAKSVREILLEVVEKSSGIAVVTTTKIILKDFATEVDESKLKTAAIIMVRHLAQSLARATSIEPLKEGIRSTMQSLAPNLMSLSSSPAEELDTAINENIGIALVLIEKASMDKSTQDLADQLMQAIAIRRYHKERRADQPFITQNTNPYSLSLPEPLGLKNTGVTPQQFRVYEEFGKNIPNLDVIPFAGLPAHAPPMTQNVGLTQPQQQQAQMPTQILTSEQIRAQQQQQQLQKSRLNQPSQSAQPPGVNVPNPQGGIAAVQSDLEQNQRVLVHLMDILVSQIKENATKNNLAELGDQNQIKTIIFQILTFIAKSAQKDQLALKVSQAVVNSLFATSESPLCREVLSLLLEKLCSLSLVARKDVVWWLVYALDSRKFNVPVIRSLLEVNLIDATELDNVLVTAMKNKMENSTEFAMKLIQNTVLSDDPILMRMDFIKTLEHLASSEDENVKKFIKEFEDTKIMPVRKGTKTTRTEKLYLVFTEWVKLLQRVENNDVITTVFIKQLVEKGVISDTDNLLTFVKSSLELSVSSFKESDPTDEVFIAIDALGSLIIKLLILQGFKDDTRRDYINAIFSVIVLVFAKDHSQEGTTFNERPYFRLFSNILYEWATIRTHNFVRISDSSTRQELIEFDSVFYNTFSGYLHALQPFAFPGFSFAWVTLLSHRMLLPIMLRLPNKIGWEKLMLLIIDLFKFLDQYTSKHAVSDAVSVVYKGTLRVILGISNDMPSFLIENHYELMNNLPPTYFQLKNVILSAIPKNMTVPNPYDVDLNMEDIPACKELPEVFFDPVIDLHSLKKPVDNYLRIPSNSLLRTILSAIYKDTYDIKKGVGYDFLSVDSKLIRAIVLHVGIEAGIEYKRTSSNAVFNTKSSYYTLLFNLIQNGSIEMKYQIILSIVEQLRYPNIHTYWFSFVLMNMFKSDEWNDQKLEVQEIILRNFLKRIIVNKPHTWGVSVFFTQLINNNDINLLDLPFVQSVPEIKLILQQLVKYSKKYTTSEQDDQSATINRRQTPLQSNA

Target ORF information:

RefSeq Version	NM_001178799.1
Organism	Saccharomyces cerevisiae S288C
Definition	Saccharomyces cerevisiae S288C CCR4-NOT core subunit CDC39 (CDC39), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001178799.1

ORF Insert Sequence:

ATGCTATCGG CCACATACCG TGATTTGAAC ACAGCATCTA ATTTAGAAAC ATCAAAGGAA 
AAACAGGCCG CTCAAATCGT CATTGCACAA ATTAGTTTAT TATTCACGAC TCTTAACAAC 
GACAATTTTG AATCCGTGGA AAGAGAAATT AGACATATTT TAGACAGGTC GTCCGTAGAT 
ATTTACATAA AAGTTTGGGA ACGATTATTA ACCTTAAGTT CTCGGGATAT TTTACAAGCG 
GGAAAATTTT TACTTCAAGA AAATCTACTA CACAGACTAC TATTAGAATT TGCGAAGGAT 
TTACCGAAGA AAAGCACAGA CCTTATTGAG CTTTTGAAAG AACGAACCTT CAATAACCAG 
GAGTTTCAAA AACAAACAGG AATTACATTA TCACTTTTCA TTGATCTATT TGATAAATCT 
GCAAACAAGG ACATTATAGA GTCACTTGAC CGCTCCTCTC AGATTAACGA TTTCAAGACA 
ATTAAGATGA ATCATACAAA TTATTTAAGG AATTTTTTTC TTCAAACCAC ACCAGAAACA 
CTAGAGTCCA ATCTACGCGA CTTATTGCAT TCCTTGGAAG GTGAAAGTCT AAATGACTTA 
TTAGCTCTTT TACTGTCCGA AATACTTTCA CCTGGGTCTC AGAATTTACA AAATGATCCC 
ACACGGAGTT GGTTGACACC TCCGATGGTT TTAGACGCAA CGAACCGTGG GAACGTTATA 
GCAAGATCTA TAAGTTCTCT GCAAGCCAAC CAGATAAATT GGAATCGTGT GTTTAATTTA 
ATGTCAACAA AGTATTTCTT GAGCGCACCA TTGATGCCTA CTACAGCATC TTTGAGTTGC 
TTATTTGCAG CATTGCACGA TGGTCCAGTT ATTGATGAAT TTTTCAGTTG CGACTGGAAA 
GTTATTTTCA AACTAGATTT GGCCATTCAA CTTCATAAGT GGTCGGTACA GAATGGTTGC 
TTTGACTTAT TAAATGCAGA AGGTACCAGG AAAGTTTCTG AAACCATCCC AAACACAAAG 
CAATCTTTAC TCTACTTATT ATCCATTGCA TCATTGAATT TAGAATTGTT CCTACAAAGG 
GAGGAATTGT CTGATGGTCC TATGCTAGCT TATTTTCAAG AGTGCTTCTT TGAAGATTTC 
AACTACGCCC CTGAATATCT TATTTTAGCA TTAGTCAAAG AAATGAAGCG GTTCGTTTTA 
TTGATAGAAA ACAGGACAGT CATAGACGAA ATACTTATTA CCTTATTGAT TCAAGTGCAT 
AATAAATCAC CGTCATCGTT CAAGGACGTT ATTTCTACAA TAACCGATGA TTCTAAAATC 
GTAGATGCAG CAAAAATCAT AATCAACTCG GATGACGCAC CTATTGCCAA CTTTTTAAAA 
TCGTTGTTAG ATACGGGAAG ATTAGATACG GTCATTAATA AACTTCCTTT CAATGAAGCT 
TTTAAAATTT TGCCATGCGC AAGACAAATT GGTTGGGAGG GGTTCGATAC TTTCTTAAAA 
ACAAAAGTTT CTCCATCTAA TGTCGATGTA GTGCTGGAAT CACTAGAGGT TCAAACGAAA 
ATGACTGATA CAAACACTCC ATTTAGGTCA TTAAAGACAT TTGACTTATT CGCTTTTCAT 
TCATTAATTG AAGTACTGAA CAAATGCCCA CTAGATGTTC TCCAATTACA AAGGTTTGAA 
TCCTTGGAAT TTTCCTTATT AATTGCATTT CCTAGATTGA TCAATTTTGG TTTTGGACAC 
GATGAAGCTA TTTTAGCCAA TGGTGACATC GCAGGGATTA ATAATGATAT TGAAAAGGAG 
ATGCAGAACT ATTTACAGAA AATGTATAGT GGTGAGTTAG CCATTAAAGA TGTAATCGAA 
CTTCTGAGAA GGTTAAGAGA TAGCGACTTG CCAAGGGACC AGGAAGTCTT CACATGTATT 
ACCCATGCCG TTATAGCAGA ATCGACATTC TTCCAAGATT ATCCATTGGA TGCATTGGCT 
ACTACATCTG TTCTTTTTGG ATCCATGATT CTCTTTCAAC TGTTACGTGG ATTCGTATTA 
GACGTCGCAT TTAGGATAAT CATGAGGTTT GCCAAGGAGC CTCCAGAGTC CAAGATGTTT 
AAGTTTGCTG TACAAGCTAT TTATGCATTT AGGATACGTT TGGCCGAATA TCCACAGTAT 
TGTAAGGACC TCTTGAGAGA TGTTCCGGCT TTGAAGTCTC AGGCTCAAGT TTACCAATCT 
ATCGTCGAAG CTGCTACCCT AGCAAATGCT CCAAAGGAAA GGTCAAGACC CGTCCAGGAA 
ATGATCCCAT TAAAATTTTT TGCTGTAGAT GAAGTTTCAT GTCAGATCAA TCAAGAAGGT 
GCTCCTAAAG ATGTCGTAGA AAAAGTTCTT TTTGTTCTCA ACAACGTTAC TCTGGCTAAC 
TTGAATAATA AGGTTGATGA ATTGAAAAAA AGTTTGACAC CAAATTATTT TTCTTGGTTT 
TCCACATATT TAGTTACGCA AAGGGCTAAA ACAGAACCTA ACTATCATGA TCTTTATAGC 
AAGGTTATAG TTGCTATGGG GTCAGGGTTG CTACATCAGT TCATGGTCAA CGTTACTTTG 
AGACAATTAT TTGTCCTACT ATCTACAAAA GACGAGCAAG CCATCGATAA AAAGCACCTA 
AAGAATTTGG CTTCATGGTT AGGATGTATC ACATTAGCTT TGAATAAACC AATTAAACAC 
AAGAATATCG CATTCAGGGA AATGTTAATC GAAGCTTATA AGGAAAATAG ACTTGAAATA 
GTTGTGCCTT TTGTAACAAA GATTTTACAA AGGGCTTCTG AATCAAAAAT TTTCAAGCCT 
CCAAATCCCT GGACTGTTGG CATATTAAAG CTGTTGATTG AGTTGAACGA AAAAGCAAAC 
TGGAAATTAA GTTTGACTTT CGAAGTTGAG GTTTTATTAA AATCTTTTAA TTTGACCACC 
AAATCTCTCA AGCCCTCGAA TTTCATCAAT ACTCCGGAAG TTATAGAAAC TTTATCCGGT 
GCTTTGGGAT CAATCACTCT GGAGCAACAA CAAACAGAGC AACAAAGGCA AATTATACTA 
ATGCAACAAC ACCAGCAACA GATGCTAATA TATCAACAGA GACAACAACA ACAACAACAA 
AGGCAACAAC AACAACAACA TCATATTAGT GCAAATACAA TCGCAGACCA ACAAGCGGCA 
TTTGGCGGCG AGGGTTCAAT TTCACACGAC AATCCTTTTA ACAACTTACT TGGTTCTACT 
ATTTTTGTAA CCCACCCTGA CTTGAAGAGG GTATTTCAAA TGGCTTTAGC CAAGTCAGTT 
CGCGAAATTT TGTTGGAAGT AGTCGAAAAG TCATCAGGAA TTGCTGTTGT TACGACGACA 
AAAATAATAC TTAAAGACTT TGCCACTGAA GTTGATGAGT CTAAGTTGAA GACGGCTGCA 
ATCATTATGG TAAGGCATTT GGCACAAAGT TTAGCTCGAG CTACTTCAAT TGAACCATTG 
AAAGAAGGCA TACGTTCTAC TATGCAATCA CTAGCACCGA ATTTAATGTC TCTTTCTTCT 
TCACCTGCAG AGGAGCTTGA CACGGCAATA AATGAAAATA TTGGCATTGC TCTAGTTTTG 
ATTGAGAAAG CATCTATGGA CAAGTCTACT CAAGATTTAG CAGACCAATT GATGCAAGCG 
ATTGCTATTC GTCGTTATCA CAAGGAAAGA AGGGCAGACC AACCATTTAT TACGCAAAAT 
ACCAATCCAT ATTCACTGTC TTTACCAGAA CCTCTTGGTT TGAAAAACAC TGGTGTTACT 
CCTCAACAAT TCAGGGTATA CGAAGAATTT GGTAAGAATA TTCCAAACTT GGATGTTATT 
CCGTTTGCAG GATTGCCCGC TCACGCTCCA CCGATGACTC AAAATGTGGG TTTAACTCAG 
CCTCAGCAAC AACAAGCGCA AATGCCTACC CAAATCCTAA CCTCCGAACA AATAAGAGCT 
CAACAACAAC AGCAGCAATT ACAGAAAAGC CGTTTGAATC AGCCATCCCA GTCGGCTCAA 
CCTCCAGGAG TGAATGTCCC AAATCCTCAA GGTGGGATTG CTGCAGTTCA ATCAGATTTG 
GAACAGAATC AACGTGTTCT CGTTCACCTC ATGGACATTT TAGTTTCTCA AATTAAAGAA 
AATGCTACGA AGAATAACTT AGCTGAATTA GGCGATCAAA ACCAAATTAA AACCATCATT 
TTTCAAATTT TGACATTCAT TGCAAAAAGC GCACAAAAGG ATCAATTAGC TTTAAAGGTA 
TCCCAAGCTG TCGTTAATAG CCTTTTTGCC ACTAGTGAGA GTCCTCTCTG CAGAGAAGTT 
TTGTCCCTAC TTTTGGAAAA GTTATGTTCT TTATCCCTCG TTGCTAGAAA AGACGTTGTC 
TGGTGGTTAG TTTATGCCTT GGACAGTAGG AAATTCAATG TTCCCGTTAT CAGATCCCTT 
CTAGAAGTTA ATTTAATTGA TGCTACAGAA TTAGATAACG TTTTAGTTAC TGCAATGAAA 
AATAAAATGG AGAACTCAAC TGAATTTGCT ATGAAATTAA TTCAGAATAC TGTCTTGTCT 
GATGATCCAA TTTTGATGAG AATGGACTTC ATTAAAACCT TAGAACACTT GGCCTCTTCG 
GAAGATGAAA ATGTAAAGAA ATTCATCAAA GAGTTCGAAG ATACTAAGAT AATGCCAGTG 
AGGAAAGGTA CCAAAACCAC AAGAACAGAA AAGCTTTACT TAGTATTTAC GGAATGGGTA 
AAATTACTTC AAAGAGTTGA GAATAACGAC GTAATCACAA CTGTTTTTAT CAAGCAATTA 
GTCGAAAAGG GTGTTATCAG CGATACTGAT AATTTACTTA CATTTGTCAA AAGTTCTCTT 
GAGCTATCAG TTTCTTCATT CAAAGAAAGT GACCCGACTG ATGAGGTTTT CATCGCTATT 
GATGCTCTAG GATCGCTAAT TATAAAATTG TTGATTTTAC AGGGTTTCAA AGATGATACA 
AGAAGAGATT ACATAAATGC AATATTTTCT GTGATCGTTT TAGTGTTTGC TAAGGATCAT 
AGCCAAGAGG GTACCACATT CAATGAACGA CCATATTTCA GACTATTTTC TAACATCTTA 
TACGAATGGG CTACCATCAG GACGCACAAT TTTGTTAGAA TATCTGATTC CAGCACTAGG 
CAGGAGCTGA TCGAATTTGA TTCTGTATTT TACAACACTT TCTCAGGATA TTTGCACGCT 
CTGCAACCAT TTGCCTTCCC TGGATTCTCA TTTGCATGGG TGACACTATT ATCACACAGA 
ATGTTATTAC CAATTATGCT AAGATTACCC AATAAAATAG GTTGGGAAAA GTTAATGCTT 
TTGATTATCG ATTTGTTTAA ATTTTTGGAC CAATACACAA GTAAACATGC AGTCTCTGAC 
GCTGTTTCGG TTGTTTATAA GGGAACACTG CGTGTTATTT TAGGCATTTC GAATGATATG 
CCATCCTTTT TGATTGAAAA TCACTATGAA TTAATGAACA ATCTACCTCC AACATATTTC 
CAACTAAAGA ATGTTATTTT ATCTGCTATT CCTAAGAATA TGACCGTTCC CAACCCATAT 
GACGTGGATC TTAATATGGA GGATATTCCA GCATGTAAAG AACTACCTGA AGTCTTCTTT 
GATCCTGTAA TTGATTTACA CTCATTGAAA AAGCCAGTTG ACAACTACCT ACGTATTCCC 
TCAAATTCAT TATTAAGAAC AATACTAAGC GCTATTTACA AGGATACCTA TGACATAAAA 
AAGGGCGTAG GCTACGACTT TTTATCTGTT GATAGTAAAT TAATTCGCGC TATTGTATTA 
CATGTGGGCA TTGAAGCTGG AATAGAGTAT AAGAGAACTT CTTCAAATGC GGTATTTAAT 
ACGAAGTCTT CTTATTATAC TTTATTGTTC AATCTGATTC AAAATGGTAG CATCGAAATG 
AAATATCAAA TTATTCTGTC TATTGTGGAA CAATTGCGGT ATCCAAACAT CCACACCTAT 
TGGTTCAGCT TTGTGTTAAT GAATATGTTC AAAAGTGACG AATGGAATGA TCAAAAACTT 
GAAGTCCAAG AAATTATTTT AAGAAACTTT TTAAAAAGAA TTATTGTTAA CAAACCACAT 
ACCTGGGGTG TTTCAGTTTT CTTTACTCAG TTGATAAACA ATAACGATAT TAATCTTTTA 
GACCTGCCCT TTGTACAAAG TGTTCCCGAA ATTAAACTAA TTTTACAACA ATTAGTAAAA 
TATTCCAAAA AATACACAAC CAGTGAACAA GATGACCAAT CCGCCACCAT CAATAGAAGG 
CAAACCCCTC TACAATCCAA CGCATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Life with 6000 genes.
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)
Goffeau A,Barrell BG,Bussey H,Davis RW,Dujon B,Feldmann H,Galibert F,Hoheisel JD,Jacq C,Johnston M,Louis EJ,Mewes HW,Murakami Y,Philippsen P,Tettelin H,Oliver SG

The complete DNA sequence of yeast chromosome III.
Nature357(6373)38-46(1992 May)
Oliver SG,van der Aart QJ,Agostoni-Carbone ML,Aigle M,Alberghina L,Alexandraki D,Antoine G,Anwar R,Ballesta JP,Benit P

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CDC39 cDNA ORF clone, Saccharomyces cerevisiae S288C

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gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

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