Tie1 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Tie1
Entrez Gene ID	89806
Full Name	tyrosine kinase with immunoglobulin-like and EGF-like domains 1
General protein information	Preferred Names tyrosine kinase with immunoglobulin-like and EGF-like domains 1 Names tyrosine-protein kinase receptor Tie-1 tyrosine kinase receptor 1
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 5 Map Location: 5q36
Summary	essential for vascular development and remodeling in the embryo; may mediate maintenance and repair of the adult vascular system [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
XM_006238720.3	XP_006238782.1	tyrosine-protein kinase receptor Tie-1 isoform X1
NM_053545.1	NP_445997.1	tyrosine-protein kinase receptor Tie-1 precursor

Pathways from BioSystems

Homologies

Bos taurus (cattle)	TIE1	NP_776390.1
Rattus norvegicus (Norway rat)	Tie1	NP_445997.1
Gallus gallus (chicken)	TIE1	XP_004936746.1
Danio rerio (zebrafish)	tie1	XP_001334673.5
Pan troglodytes (chimpanzee)	TIE1	XP_001173341.1
Canis lupus familiaris (dog)	TIE1	XP_539652.3
Mus musculus (house mouse)	Tie1	NP_035717.2
Xenopus tropicalis (tropical clawed frog)	tie1	XP_002931650.2
Homo sapiens (human)	TIE1	NP_005415.1

Gene Ontology
Bibliography

Related articles in PubMed

Conditionals by inversion provide a universal method for the generation of conditional alleles.
Economides AN, Frendewey D, Yang P, Dominguez MG, Dore AT, Lobov IB, Persaud T, Rojas J, McClain J, Lengyel P, Droguett G, Chernomorsky R, Stevens S, Auerbach W, Dechiara TM, Pouyemirou W, Cruz JM Jr, Feeley K, Mellis IA, Yasenchack J, Hatsell SJ, Xie L, Latres E, Huang L, Zhang Y, Pefanis E, Skokos D, Deckelbaum RA, Croll SD, Davis S, Valenzuela DM, Gale NW, Murphy AJ, Yancopoulos GD
Proceedings of the National Academy of Sciences of the United States of America110(34)E3179-88(2013 Aug)

Retinoic acid receptor gamma activates receptor tyrosine kinase Tie1 gene transcription through transcription factor GATA4 in F9 stem cells.
Su D, Gudas LJ
Experimental hematology36(5)624-41(2008 May)

Inhibitor of differentiation 1 promotes endothelial survival in a bleomycin model of lung injury in mice.
Zhang H, Lawson WE, Polosukhin VV, Pozzi A, Blackwell TS, Litingtung Y, Chiang C
The American journal of pathology171(4)1113-26(2007 Oct)

Stretch induces upregulation of key tyrosine kinase receptors in microvascular endothelial cells.
Zheng W, Christensen LP, Tomanek RJ
American journal of physiology. Heart and circulatory physiology287(6)H2739-45(2004 Dec)

Enhanced expression of angiopoietin-2 and the Tie2 receptor but not angiopoietin-1 or the Tie1 receptor in a rat model of myocardial infarction.
Shyu KG, Liang YJ, Chang H, Wang BW, Leu JG, Kuan P
Journal of biomedical science11(2)163-71(2004 Mar-Apr)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Mechanical stretch induces upregulation of the key tyrosine kinase receptors Flk-1, Tie-2, and Tie-1 in vascular endothelial cells
Title: Stretch induces upregulation of key tyrosine kinase receptors in microvascular endothelial cells.

Ang2 and Tie2 expressions significantly increased both spatial and temporal patterns after myocardial infarction in the rat ventricular myocardium, while Ang1 and Tie1 receptor expression did not.
Title: Enhanced expression of angiopoietin-2 and the Tie2 receptor but not angiopoietin-1 or the Tie1 receptor in a rat model of myocardial infarction.

The following Tie1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Tie1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa09848	NM_053545.1 Latest version!	Rattus norvegicus tyrosine kinase with immunoglobulin-like and EGF-like domains 1 (Tie1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$559.30 $799.00
ORa28509	XM_006238720.3 Latest version!	Rattus norvegicus tyrosine kinase with immunoglobulin-like and EGF-like domains 1 (Tie1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$559.30 $799.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Tie1 ( NM_053545.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_445997.1 Rattus norvegicus tyrosine kinase with immunoglobulin-like and EGF-like domains 1 (Tie1), mRNA.

Price & Availability↑

CloneID	ORa09848
Clone ID Related Accession (Same CDS sequence)	NM_053545.1
Accession Version	NM_053545.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3414bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-27
Organism	Rattus norvegicus(Norway rat)
Product	tyrosine-protein kinase receptor Tie-1 precursor
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC169020.1. On or before Sep 5, 2008 this sequence version replaced XM_233462.4, XM_001069541.1. ##Evidence-Data-START## Transcript exon combination :: BC169020.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMD00052296, SAMD00052297 [ECO:0000350] ##Evidence-Data-END##

ATGGTCTGGT GGGGATCCCC TTTCCTGCTC CCCATTCTTT TTCTGGCCTC TCATGTTGGT 
GCATCCGTGG ATTTAACACT GCTGGCCAAT CTGCGTATCA CCGAACCCCA GCGTTTCTTC 
CTGACCTGTG TGTCTGGTGA GGCGGGAGCA GGGAGGAGCT CCGACGTCTG GGGCCCGCCC 
CTGCTGCTGG AAAAGGATGA TCGCATAGTG CGCACCTTCC CACCCGGGCA GCCCCTGCAC 
CTCACTCGCA ACGGTTCACA CCAGGTCACG CTGCGGGGCT TCTCCAAGCC CTCGGATTTA 
GTAGGCGTCT TCTCCTGCGT GGGTGGAGCT GGCACGAGGC GCACTCGAGT CCTCTATGTG 
CACAACAGCC CGGGGGCACA CCTGTTTCCA GACAAGGTCA CACACACGGT GAACAAAGGT 
GATACCGCTG TGCTTTCTGC ACGAGTGCAC AAGGAAAAGC AGACTGATGT GATTTGGAAG 
AACAATGGAT CCTACTTCCA TACCCTAGAC TGGCATGAGG CCCATGATGG GCGGTTTCAA 
CTGCAGCTCC AAAATGTACA GCCACCATCG AGTGGCATCT ACAGCGCCAC CTACCTAGAG 
GCCAGCCCCT TGGGCAGTGC CTTCTTTCGG CTCATCGTGC GAGGCTGTGA GGCTGGACGC 
TGGGGGCCGG GATGTGTCAA GGATTGCCCA GGCTGCCTGC ATGGAGGTGT CTGTCATGAC 
CACGATGGCG AATGTGTGTG CCCCCCTGGA TTCACTGGCA CCCGCTGTGA GCAGGCCTGC 
AGAGAAGGTC GTTTTGGACA GAGCTGCCAG GAACAGTGCC CAGGCACAGC AGGCTGTCGG 
GGTCTCACCT TCTGCCTCCC AGATCCCTAT GGCTGTTCTT GTGGATCTGG CTGGAGGGGA 
AGCCAGTGCC AGGAAGCATG TGCACCTGGT CATTTTGGGG CAGATTGTCG CCTCCAGTGT 
CAGTGTCAAA ATGGTGGTAC TTGTGATCGG TTCAGCGGCT GCGTCTGCCC CTCCGGGTGG 
CATGGAGTCC ACTGTGAAAA GTCAGACCGG ATCCCCCAGA TCCTCAATGT GGCCACAGAG 
CTGGAGTTCA ACTTAGGGAC AATGCCCCGG ATCAACTGTG CAGCTGCCGG GAATCCCTTC 
CCAGTACGGG GCAGCATGGA ACTCCGCAAG CCAGACGGCA CCATGCTCCT GTCTACCAAA 
GCCATTGTGG AGCCAGATAG GACCACAGCT GAGTTCGAGG TGCCCCGTTT GACTCTTGGG 
GACAGCGGGT TCTGGGAATG CCGTGTATCG ACGTCTGGTG GCCAAGATAG CCGGCGCTTC 
AAGGTCAATG TCAAAGTACC CCCAGTGCCT CTGACTGCAC CTCGGCTCCT GGCCAAGCAG 
AGCCGCCAGC TTGTGGTCTC CCCACTGGTC TCCTTCGGTG GGGATGGACC CATCTCCTCT 
GTCCGCCTGC ACTACCGGCC CCAGGACAGC ATGATTACCT GGTCTGCCAT TGTGGTGGAT 
CCCAGTGAGA ATGTGACATT AATGAACCTG AAGCCAAGGA CAGGATACAA CGTTCGGGTG 
CAGCTGAGCC GGCCAGGGGA AGGAGGAGAA GGAGCCTGGG GTCCTTCGAC TCTCATGACT 
ACGGACTGTC CTGAGCCTTT GCTGCAGCCG TGGGTAGAGA GCTGGAACGT GGAGGGTCCT 
GACAGGCTAC GGGTGAGCTG GTCTCTACCC TCGGTGCCAC TGTCTGGGGA TGGTTTCCTG 
CTGCGTCTGT GGGATGGGGC CCGGGGACAG GAGAGGCGGG AGAACATCTC ATCCCCCCAG 
GCCCGCACTG CCCTCCTGAC TGGACTCACG CCTGGCACGC ACTACCAACT GGATGTGCGG 
CTGTATCACT GCACCCTCCT GGGCCCTGCC TCACCCTCTG CGCATGTGCA CCTGCCCCTC 
AGCGGGCCTC CAGCTCCCCG CCACCTCCGT GCCCAGGCCC TCTCAGACTC TGAGATCCGT 
CTGATGTGGC AGCATCCTGA GGCTCCGCCT GGACCTATAT CCAAGTACAT TGTGGAGATT 
CAGGTGGCCG GGGGCTCAGG AGACCCTCAG TGGATGGATG TAGACAAACC CGAGGAGACG 
AGCACCACCG TCCGTGGTCT CAATGCCAGC ACACGCTACC TCTTCCGTGT GCGGGCCAGT 
GTGCAGGGTC TCGGCGACTG GAGCAACACA GTGGAAGAGA CCACGCTGGG TAATGGGCTG 
CAGAGTGCGA GCCCAGTCCA AGAGAGCCGG GTGGCTGAAG ACGGTCTGGA TCAGCAGCTG 
GTCCTGGCTG TGGTAGGCTC CGTCTCTGCC ACCTGCCTCA CCATCCTGGC TGCCCTTTTA 
GCCCTGGTGT GTATCCGAAG AAGCTGCCTA CATCGGAGAC ATACCTTCAC CTACCAGTCA 
GGATCGGGTG AAGAGACCAT CCTGCAGTTC AGCTCAGGGA CCTTGACATT GACCCGACGG 
CCAAAGCCAC AGCCTGAGCC TTTGAGTTAC CCTGTGCTGG AGTGGGAGGA CATCACCTTT 
GAGGACCTCA TCGGGGAGGG GAACTTCGGC CAAGTGATCC GGGCCATGAT CAAGAAGGAC 
GGACTCAAGA TGAACGCAGC CATCAAGATG CTGAAAGAGT ATGCGTCTGA AAATGACCAC 
CGAGACTTTG CAGGGGAACT AGAAGTTCTG TGCAAACTAG GACACCACCC CAATATTATC 
AACCTCTTGG GGGCCTGTGA GAACCGAGGC TACTTGTATA TTGCGATCGA ATATGCCCCG 
TATGGAAACC TGCTAGATTT CCTGAGAAAG AGCAGGGTCC TGGAGACTGA TCCAGCTTTT 
GCTCGAGAGC ATGGAACAGC CTCCACACTC AGCTCCCGGC AGCTGCTGCG CTTTGCCAGT 
GATGCAGCCA ACGGGATGCA GTACCTTAGT GAGAAGCAGT TCATTCACAG GGACCTGGCT 
GCCCGAAATG TTCTGGTCGG AGAGAACCTG GCCTCCAAGA TTGCAGACTT TGGCCTTTCT 
CGGGGGGAGG AAGTATATGT GAAGAAGACG ATGGGCCGTC TTCCTGTGCG TTGGATGGCC 
ATTGAGTCCC TCAACTACAG CGTCTACACT ACCAAGAGTG ACGTCTGGTC ATTTGGGGTC 
CTCCTCTGGG AGATAGTGAG CCTTGGAGGC ACGCCCTACT GTGGCATGAC CTGTGCTGAG 
CTCTACGAGA AACTGCCTCA AGGCTATCGC ATGGAGCAGC CTCGAAACTG TGATGATGAA 
GTGTACGAGC TGATGCGGCA GTGCTGGCGG GACCGTCCCT ATGAGCGCCC CCCTTTTGCC 
CAGATTGCAC TACAGTTGGG CCGCATGCTG GAAGCCAGGA AGGCCTACGT GAACATGTCA 
CTGTTTGAGA ACTTTACCTA TGCAGGCATC GATGCCACAG CTGAGGAGGC CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_445997.1
CDS	51..3464
Translation	MVWWGSPFLLPILFLASHVGASVDLTLLANLRITEPQRFFLTCVSGEAGAGRSSDVWGPPLLLEKDDRIVRTFPPGQPLHLTRNGSHQVTLRGFSKPSDLVGVFSCVGGAGTRRTRVLYVHNSPGAHLFPDKVTHTVNKGDTAVLSARVHKEKQTDVIWKNNGSYFHTLDWHEAHDGRFQLQLQNVQPPSSGIYSATYLEASPLGSAFFRLIVRGCEAGRWGPGCVKDCPGCLHGGVCHDHDGECVCPPGFTGTRCEQACREGRFGQSCQEQCPGTAGCRGLTFCLPDPYGCSCGSGWRGSQCQEACAPGHFGADCRLQCQCQNGGTCDRFSGCVCPSGWHGVHCEKSDRIPQILNVATELEFNLGTMPRINCAAAGNPFPVRGSMELRKPDGTMLLSTKAIVEPDRTTAEFEVPRLTLGDSGFWECRVSTSGGQDSRRFKVNVKVPPVPLTAPRLLAKQSRQLVVSPLVSFGGDGPISSVRLHYRPQDSMITWSAIVVDPSENVTLMNLKPRTGYNVRVQLSRPGEGGEGAWGPSTLMTTDCPEPLLQPWVESWNVEGPDRLRVSWSLPSVPLSGDGFLLRLWDGARGQERRENISSPQARTALLTGLTPGTHYQLDVRLYHCTLLGPASPSAHVHLPLSGPPAPRHLRAQALSDSEIRLMWQHPEAPPGPISKYIVEIQVAGGSGDPQWMDVDKPEETSTTVRGLNASTRYLFRVRASVQGLGDWSNTVEETTLGNGLQSASPVQESRVAEDGLDQQLVLAVVGSVSATCLTILAALLALVCIRRSCLHRRHTFTYQSGSGEETILQFSSGTLTLTRRPKPQPEPLSYPVLEWEDITFEDLIGEGNFGQVIRAMIKKDGLKMNAAIKMLKEYASENDHRDFAGELEVLCKLGHHPNIINLLGACENRGYLYIAIEYAPYGNLLDFLRKSRVLETDPAFAREHGTASTLSSRQLLRFASDAANGMQYLSEKQFIHRDLAARNVLVGENLASKIADFGLSRGEEVYVKKTMGRLPVRWMAIESLNYSVYTTKSDVWSFGVLLWEIVSLGGTPYCGMTCAELYEKLPQGYRMEQPRNCDDEVYELMRQCWRDRPYERPPFAQIALQLGRMLEARKAYVNMSLFENFTYAGIDATAEEA

Target ORF information:

RefSeq Version	NM_053545.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus tyrosine kinase with immunoglobulin-like and EGF-like domains 1 (Tie1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_053545.1

ORF Insert Sequence:

ATGGTCTGGT GGGGATCCCC TTTCCTGCTC CCCATTCTTT TTCTGGCCTC TCATGTTGGT 
GCATCCGTGG ATTTAACACT GCTGGCCAAT CTGCGTATCA CCGAACCCCA GCGTTTCTTC 
CTGACCTGTG TGTCTGGTGA GGCGGGAGCA GGGAGGAGCT CCGACGTCTG GGGCCCGCCC 
CTGCTGCTGG AAAAGGATGA TCGCATAGTG CGCACCTTCC CACCCGGGCA GCCCCTGCAC 
CTCACTCGCA ACGGTTCACA CCAGGTCACG CTGCGGGGCT TCTCCAAGCC CTCGGATTTA 
GTAGGCGTCT TCTCCTGCGT GGGTGGAGCT GGCACGAGGC GCACTCGAGT CCTCTATGTG 
CACAACAGCC CGGGGGCACA CCTGTTTCCA GACAAGGTCA CACACACGGT GAACAAAGGT 
GATACCGCTG TGCTTTCTGC ACGAGTGCAC AAGGAAAAGC AGACTGATGT GATTTGGAAG 
AACAATGGAT CCTACTTCCA TACCCTAGAC TGGCATGAGG CCCATGATGG GCGGTTTCAA 
CTGCAGCTCC AAAATGTACA GCCACCATCG AGTGGCATCT ACAGCGCCAC CTACCTAGAG 
GCCAGCCCCT TGGGCAGTGC CTTCTTTCGG CTCATCGTGC GAGGCTGTGA GGCTGGACGC 
TGGGGGCCGG GATGTGTCAA GGATTGCCCA GGCTGCCTGC ATGGAGGTGT CTGTCATGAC 
CACGATGGCG AATGTGTGTG CCCCCCTGGA TTCACTGGCA CCCGCTGTGA GCAGGCCTGC 
AGAGAAGGTC GTTTTGGACA GAGCTGCCAG GAACAGTGCC CAGGCACAGC AGGCTGTCGG 
GGTCTCACCT TCTGCCTCCC AGATCCCTAT GGCTGTTCTT GTGGATCTGG CTGGAGGGGA 
AGCCAGTGCC AGGAAGCATG TGCACCTGGT CATTTTGGGG CAGATTGTCG CCTCCAGTGT 
CAGTGTCAAA ATGGTGGTAC TTGTGATCGG TTCAGCGGCT GCGTCTGCCC CTCCGGGTGG 
CATGGAGTCC ACTGTGAAAA GTCAGACCGG ATCCCCCAGA TCCTCAATGT GGCCACAGAG 
CTGGAGTTCA ACTTAGGGAC AATGCCCCGG ATCAACTGTG CAGCTGCCGG GAATCCCTTC 
CCAGTACGGG GCAGCATGGA ACTCCGCAAG CCAGACGGCA CCATGCTCCT GTCTACCAAA 
GCCATTGTGG AGCCAGATAG GACCACAGCT GAGTTCGAGG TGCCCCGTTT GACTCTTGGG 
GACAGCGGGT TCTGGGAATG CCGTGTATCG ACGTCTGGTG GCCAAGATAG CCGGCGCTTC 
AAGGTCAATG TCAAAGTACC CCCAGTGCCT CTGACTGCAC CTCGGCTCCT GGCCAAGCAG 
AGCCGCCAGC TTGTGGTCTC CCCACTGGTC TCCTTCGGTG GGGATGGACC CATCTCCTCT 
GTCCGCCTGC ACTACCGGCC CCAGGACAGC ATGATTACCT GGTCTGCCAT TGTGGTGGAT 
CCCAGTGAGA ATGTGACATT AATGAACCTG AAGCCAAGGA CAGGATACAA CGTTCGGGTG 
CAGCTGAGCC GGCCAGGGGA AGGAGGAGAA GGAGCCTGGG GTCCTTCGAC TCTCATGACT 
ACGGACTGTC CTGAGCCTTT GCTGCAGCCG TGGGTAGAGA GCTGGAACGT GGAGGGTCCT 
GACAGGCTAC GGGTGAGCTG GTCTCTACCC TCGGTGCCAC TGTCTGGGGA TGGTTTCCTG 
CTGCGTCTGT GGGATGGGGC CCGGGGACAG GAGAGGCGGG AGAACATCTC ATCCCCCCAG 
GCCCGCACTG CCCTCCTGAC TGGACTCACG CCTGGCACGC ACTACCAACT GGATGTGCGG 
CTGTATCACT GCACCCTCCT GGGCCCTGCC TCACCCTCTG CGCATGTGCA CCTGCCCCTC 
AGCGGGCCTC CAGCTCCCCG CCACCTCCGT GCCCAGGCCC TCTCAGACTC TGAGATCCGT 
CTGATGTGGC AGCATCCTGA GGCTCCGCCT GGACCTATAT CCAAGTACAT TGTGGAGATT 
CAGGTGGCCG GGGGCTCAGG AGACCCTCAG TGGATGGATG TAGACAAACC CGAGGAGACG 
AGCACCACCG TCCGTGGTCT CAATGCCAGC ACACGCTACC TCTTCCGTGT GCGGGCCAGT 
GTGCAGGGTC TCGGCGACTG GAGCAACACA GTGGAAGAGA CCACGCTGGG TAATGGGCTG 
CAGAGTGCGA GCCCAGTCCA AGAGAGCCGG GTGGCTGAAG ACGGTCTGGA TCAGCAGCTG 
GTCCTGGCTG TGGTAGGCTC CGTCTCTGCC ACCTGCCTCA CCATCCTGGC TGCCCTTTTA 
GCCCTGGTGT GTATCCGAAG AAGCTGCCTA CATCGGAGAC ATACCTTCAC CTACCAGTCA 
GGATCGGGTG AAGAGACCAT CCTGCAGTTC AGCTCAGGGA CCTTGACATT GACCCGACGG 
CCAAAGCCAC AGCCTGAGCC TTTGAGTTAC CCTGTGCTGG AGTGGGAGGA CATCACCTTT 
GAGGACCTCA TCGGGGAGGG GAACTTCGGC CAAGTGATCC GGGCCATGAT CAAGAAGGAC 
GGACTCAAGA TGAACGCAGC CATCAAGATG CTGAAAGAGT ATGCGTCTGA AAATGACCAC 
CGAGACTTTG CAGGGGAACT AGAAGTTCTG TGCAAACTAG GACACCACCC CAATATTATC 
AACCTCTTGG GGGCCTGTGA GAACCGAGGC TACTTGTATA TTGCGATCGA ATATGCCCCG 
TATGGAAACC TGCTAGATTT CCTGAGAAAG AGCAGGGTCC TGGAGACTGA TCCAGCTTTT 
GCTCGAGAGC ATGGAACAGC CTCCACACTC AGCTCCCGGC AGCTGCTGCG CTTTGCCAGT 
GATGCAGCCA ACGGGATGCA GTACCTTAGT GAGAAGCAGT TCATTCACAG GGACCTGGCT 
GCCCGAAATG TTCTGGTCGG AGAGAACCTG GCCTCCAAGA TTGCAGACTT TGGCCTTTCT 
CGGGGGGAGG AAGTATATGT GAAGAAGACG ATGGGCCGTC TTCCTGTGCG TTGGATGGCC 
ATTGAGTCCC TCAACTACAG CGTCTACACT ACCAAGAGTG ACGTCTGGTC ATTTGGGGTC 
CTCCTCTGGG AGATAGTGAG CCTTGGAGGC ACGCCCTACT GTGGCATGAC CTGTGCTGAG 
CTCTACGAGA AACTGCCTCA AGGCTATCGC ATGGAGCAGC CTCGAAACTG TGATGATGAA 
GTGTACGAGC TGATGCGGCA GTGCTGGCGG GACCGTCCCT ATGAGCGCCC CCCTTTTGCC 
CAGATTGCAC TACAGTTGGG CCGCATGCTG GAAGCCAGGA AGGCCTACGT GAACATGTCA 
CTGTTTGAGA ACTTTACCTA TGCAGGCATC GATGCCACAG CTGAGGAGGC CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Tie1 ( XM_006238720.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006238782.1 Rattus norvegicus tyrosine kinase with immunoglobulin-like and EGF-like domains 1 (Tie1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ORa28509
Clone ID Related Accession (Same CDS sequence)	XM_006238720.3
Accession Version	XM_006238720.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3408bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	tyrosine-protein kinase receptor Tie-1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005104.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006238720.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTCTGGT GGGGATCCCC TTTCCTGCTC CCCATTCTTT TTCTGGCCTC TCATGTTGGT 
GCATCCGTGG ATTTAACACT GCTGGCCAAT CTGCGTATCA CCGAACCCCA GCGTTTCTTC 
CTGACCTGTG TGTCTGGTGA GGCGGGAGCA GGGAGGAGCT CCGACGTCTG GGGCCCGCCC 
CTGCTGCTGG AAAAGGATGA TCGCATAGTG CGCACCTTCC CACCCGGGCA GCCCCTGCAC 
CTCACTCGCA ACGGTTCACA CCAGGTCACG CTGCGGGGCT TCTCCAAGCC CTCGGATTTA 
GTAGGCGTCT TCTCCTGCGT GGGTGGAGCT GGCACGAGGC GCACTCGAGT CCTCTATGTG 
CACAACAGCC CGGGGGCACA CCTGTTTCCA GACAAGGTCA CACACACGGT GAACAAAGGT 
GATACCGCTG TGCTTTCTGC ACGAGTGCAC AAGGAAAAGC AGACTGATGT GATTTGGAAG 
AACAATGGAT CCTACTTCCA TACCCTAGAC TGGCATGAGG CCCATGATGG GCGGTTTCAA 
CTGCAGCTCC AAAATGTACA GCCACCATCG AGTGGCATCT ACAGCGCCAC CTACCTAGAG 
GCCAGCCCCT TGGGCAGTGC CTTCTTTCGG CTCATCGTGC GAGGCTGTGA GGCTGGACGC 
TGGGGGCCGG GATGTGTCAA GGATTGCCCA GGCTGCCTGC ATGGAGGTGT CTGTCATGAC 
CACGATGGCG AATGTGTGTG CCCCCCTGGA TTCACTGGCA CCCGCTGTGA GCAGGCCTGC 
AGAGAAGGTC GTTTTGGACA GAGCTGCCAG GAACAGTGCC CAGGCACAGC AGGCTGTCGG 
GGTCTCACCT TCTGCCTCCC AGATCCCTAT GGCTGTTCTT GTGGATCTGG CTGGAGGGGA 
AGCCAGTGCC AGGAAGCATG TGCACCTGGT CATTTTGGGG CAGATTGTCG CCTCCAGTGT 
CAGTGTCAAA ATGGTGGTAC TTGTGATCGG TTCAGCGGCT GCGTCTGCCC CTCCGGGTGG 
CATGGAGTCC ACTGTGAAAA GTCAGACCGG ATCCCCCAGA TCCTCAATGT GGCCACAGAG 
CTGGAGTTCA ACTTAGGGAC AATGCCCCGG ATCAACTGTG CAGCTGCCGG GAATCCCTTC 
CCAGTACGGG GCAGCATGGA ACTCCGCAAG CCAGACGGCA CCATGCTCCT GTCTACCAAA 
GCCATTGTGG AGCCAGATAG GACCACAGCT GAGTTCGAGG TGCCCCGTTT GACTCTTGGG 
GACAGCGGGT TCTGGGAATG CCGTGTATCG ACGTCTGGTG GCCAAGATAG CCGGCGCTTC 
AAGGTCAATG TCAAAGTACC CCCAGTGCCT CTGACTGCAC CTCGGCTCCT GGCCAAGCAG 
AGCCGCCAGC TTGTGGTCTC CCCACTGGTC TCCTTCGGTG GGGATGGACC CATCTCCTCT 
GTCCGCCTGC ACTACCGGCC CCAGGACAGC ATGATTACCT GGTCTGCCAT TGTGGTGGAT 
CCCAGTGAGA ATGTGACATT AATGAACCTG AAGCCAAGGA CAGGATACAA CGTTCGGGTG 
CAGCTGAGCC GGCCAGGGGA AGGAGGAGAA GGAGCCTGGG GTCCTTCGAC TCTCATGACT 
ACGGACTGTC CTGAGCCTTT GCTGCAGCCG TGGGTAGAGA GCTGGAACGT GGAGGGTCCT 
GACAGGCTAC GGGTGAGCTG GTCTCTACCC TCGGTGCCAC TGTCTGGGGA TGGTTTCCTG 
CTGCGTCTGT GGGATGGGGC CCGGGGACAG GAGAGGCGGG AGAACATCTC ATCCCCCCAG 
GCCCGCACTG CCCTCCTGAC TGGACTCACG CCTGGCACGC ACTACCAACT GGATGTGCGG 
CTGTATCACT GCACCCTCCT GGGCCCTGCC TCACCCTCTG CGCATGTGCA CCTGCCCCTC 
AGCGGGCCTC CAGCTCCCCG CCACCTCCGT GCCCAGGCCC TCTCAGACTC TGAGATCCGT 
CTGATGTGGC AGCATCCTGA GGCTCCGCCT GGACCTATAT CCAAGTACAT TGTGGAGATT 
CAGGTGGCCG GGGGCTCAGG AGACCCTCAG TGGATGGATG TAGACAAACC CGAGGAGACG 
AGCACCACCG TCCGTGGTCT CAATGCCAGC ACACGCTACC TCTTCCGTGT GCGGGCCAGT 
GTGCAGGGTC TCGGCGACTG GAGCAACACA GTGGAAGAGA CCACGCTGGG GCTGCAGAGT 
GCGAGCCCAG TCCAAGAGAG CCGGGTGGCT GAAGACGGTC TGGATCAGCA GCTGGTCCTG 
GCTGTGGTAG GCTCCGTCTC TGCCACCTGC CTCACCATCC TGGCTGCCCT TTTAGCCCTG 
GTGTGTATCC GAAGAAGCTG CCTACATCGG AGACATACCT TCACCTACCA GTCAGGATCG 
GGTGAAGAGA CCATCCTGCA GTTCAGCTCA GGGACCTTGA CATTGACCCG ACGGCCAAAG 
CCACAGCCTG AGCCTTTGAG TTACCCTGTG CTGGAGTGGG AGGACATCAC CTTTGAGGAC 
CTCATCGGGG AGGGGAACTT CGGCCAAGTG ATCCGGGCCA TGATCAAGAA GGACGGACTC 
AAGATGAACG CAGCCATCAA GATGCTGAAA GAGTATGCGT CTGAAAATGA CCACCGAGAC 
TTTGCAGGGG AACTAGAAGT TCTGTGCAAA CTAGGACACC ACCCCAATAT TATCAACCTC 
TTGGGGGCCT GTGAGAACCG AGGCTACTTG TATATTGCGA TCGAATATGC CCCGTATGGA 
AACCTGCTAG ATTTCCTGAG AAAGAGCAGG GTCCTGGAGA CTGATCCAGC TTTTGCTCGA 
GAGCATGGAA CAGCCTCCAC ACTCAGCTCC CGGCAGCTGC TGCGCTTTGC CAGTGATGCA 
GCCAACGGGA TGCAGTACCT TAGTGAGAAG CAGTTCATTC ACAGGGACCT GGCTGCCCGA 
AATGTTCTGG TCGGAGAGAA CCTGGCCTCC AAGATTGCAG ACTTTGGCCT TTCTCGGGGG 
GAGGAAGTAT ATGTGAAGAA GACGATGGGC CGTCTTCCTG TGCGTTGGAT GGCCATTGAG 
TCCCTCAACT ACAGCGTCTA CACTACCAAG AGTGACGTCT GGTCATTTGG GGTCCTCCTC 
TGGGAGATAG TGAGCCTTGG AGGCACGCCC TACTGTGGCA TGACCTGTGC TGAGCTCTAC 
GAGAAACTGC CTCAAGGCTA TCGCATGGAG CAGCCTCGAA ACTGTGATGA TGAAGTGTAC 
GAGCTGATGC GGCAGTGCTG GCGGGACCGT CCCTATGAGC GCCCCCCTTT TGCCCAGATT 
GCACTACAGT TGGGCCGCAT GCTGGAAGCC AGGAAGGCCT ACGTGAACAT GTCACTGTTT 
GAGAACTTTA CCTATGCAGG CATCGATGCC ACAGCTGAGG AGGCCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006238782.1
CDS	117..3524
Translation	MVWWGSPFLLPILFLASHVGASVDLTLLANLRITEPQRFFLTCVSGEAGAGRSSDVWGPPLLLEKDDRIVRTFPPGQPLHLTRNGSHQVTLRGFSKPSDLVGVFSCVGGAGTRRTRVLYVHNSPGAHLFPDKVTHTVNKGDTAVLSARVHKEKQTDVIWKNNGSYFHTLDWHEAHDGRFQLQLQNVQPPSSGIYSATYLEASPLGSAFFRLIVRGCEAGRWGPGCVKDCPGCLHGGVCHDHDGECVCPPGFTGTRCEQACREGRFGQSCQEQCPGTAGCRGLTFCLPDPYGCSCGSGWRGSQCQEACAPGHFGADCRLQCQCQNGGTCDRFSGCVCPSGWHGVHCEKSDRIPQILNVATELEFNLGTMPRINCAAAGNPFPVRGSMELRKPDGTMLLSTKAIVEPDRTTAEFEVPRLTLGDSGFWECRVSTSGGQDSRRFKVNVKVPPVPLTAPRLLAKQSRQLVVSPLVSFGGDGPISSVRLHYRPQDSMITWSAIVVDPSENVTLMNLKPRTGYNVRVQLSRPGEGGEGAWGPSTLMTTDCPEPLLQPWVESWNVEGPDRLRVSWSLPSVPLSGDGFLLRLWDGARGQERRENISSPQARTALLTGLTPGTHYQLDVRLYHCTLLGPASPSAHVHLPLSGPPAPRHLRAQALSDSEIRLMWQHPEAPPGPISKYIVEIQVAGGSGDPQWMDVDKPEETSTTVRGLNASTRYLFRVRASVQGLGDWSNTVEETTLGLQSASPVQESRVAEDGLDQQLVLAVVGSVSATCLTILAALLALVCIRRSCLHRRHTFTYQSGSGEETILQFSSGTLTLTRRPKPQPEPLSYPVLEWEDITFEDLIGEGNFGQVIRAMIKKDGLKMNAAIKMLKEYASENDHRDFAGELEVLCKLGHHPNIINLLGACENRGYLYIAIEYAPYGNLLDFLRKSRVLETDPAFAREHGTASTLSSRQLLRFASDAANGMQYLSEKQFIHRDLAARNVLVGENLASKIADFGLSRGEEVYVKKTMGRLPVRWMAIESLNYSVYTTKSDVWSFGVLLWEIVSLGGTPYCGMTCAELYEKLPQGYRMEQPRNCDDEVYELMRQCWRDRPYERPPFAQIALQLGRMLEARKAYVNMSLFENFTYAGIDATAEEA

Target ORF information:

RefSeq Version	XM_006238720.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus tyrosine kinase with immunoglobulin-like and EGF-like domains 1 (Tie1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006238720.3

ORF Insert Sequence:

ATGGTCTGGT GGGGATCCCC TTTCCTGCTC CCCATTCTTT TTCTGGCCTC TCATGTTGGT 
GCATCCGTGG ATTTAACACT GCTGGCCAAT CTGCGTATCA CCGAACCCCA GCGTTTCTTC 
CTGACCTGTG TGTCTGGTGA GGCGGGAGCA GGGAGGAGCT CCGACGTCTG GGGCCCGCCC 
CTGCTGCTGG AAAAGGATGA TCGCATAGTG CGCACCTTCC CACCCGGGCA GCCCCTGCAC 
CTCACTCGCA ACGGTTCACA CCAGGTCACG CTGCGGGGCT TCTCCAAGCC CTCGGATTTA 
GTAGGCGTCT TCTCCTGCGT GGGTGGAGCT GGCACGAGGC GCACTCGAGT CCTCTATGTG 
CACAACAGCC CGGGGGCACA CCTGTTTCCA GACAAGGTCA CACACACGGT GAACAAAGGT 
GATACCGCTG TGCTTTCTGC ACGAGTGCAC AAGGAAAAGC AGACTGATGT GATTTGGAAG 
AACAATGGAT CCTACTTCCA TACCCTAGAC TGGCATGAGG CCCATGATGG GCGGTTTCAA 
CTGCAGCTCC AAAATGTACA GCCACCATCG AGTGGCATCT ACAGCGCCAC CTACCTAGAG 
GCCAGCCCCT TGGGCAGTGC CTTCTTTCGG CTCATCGTGC GAGGCTGTGA GGCTGGACGC 
TGGGGGCCGG GATGTGTCAA GGATTGCCCA GGCTGCCTGC ATGGAGGTGT CTGTCATGAC 
CACGATGGCG AATGTGTGTG CCCCCCTGGA TTCACTGGCA CCCGCTGTGA GCAGGCCTGC 
AGAGAAGGTC GTTTTGGACA GAGCTGCCAG GAACAGTGCC CAGGCACAGC AGGCTGTCGG 
GGTCTCACCT TCTGCCTCCC AGATCCCTAT GGCTGTTCTT GTGGATCTGG CTGGAGGGGA 
AGCCAGTGCC AGGAAGCATG TGCACCTGGT CATTTTGGGG CAGATTGTCG CCTCCAGTGT 
CAGTGTCAAA ATGGTGGTAC TTGTGATCGG TTCAGCGGCT GCGTCTGCCC CTCCGGGTGG 
CATGGAGTCC ACTGTGAAAA GTCAGACCGG ATCCCCCAGA TCCTCAATGT GGCCACAGAG 
CTGGAGTTCA ACTTAGGGAC AATGCCCCGG ATCAACTGTG CAGCTGCCGG GAATCCCTTC 
CCAGTACGGG GCAGCATGGA ACTCCGCAAG CCAGACGGCA CCATGCTCCT GTCTACCAAA 
GCCATTGTGG AGCCAGATAG GACCACAGCT GAGTTCGAGG TGCCCCGTTT GACTCTTGGG 
GACAGCGGGT TCTGGGAATG CCGTGTATCG ACGTCTGGTG GCCAAGATAG CCGGCGCTTC 
AAGGTCAATG TCAAAGTACC CCCAGTGCCT CTGACTGCAC CTCGGCTCCT GGCCAAGCAG 
AGCCGCCAGC TTGTGGTCTC CCCACTGGTC TCCTTCGGTG GGGATGGACC CATCTCCTCT 
GTCCGCCTGC ACTACCGGCC CCAGGACAGC ATGATTACCT GGTCTGCCAT TGTGGTGGAT 
CCCAGTGAGA ATGTGACATT AATGAACCTG AAGCCAAGGA CAGGATACAA CGTTCGGGTG 
CAGCTGAGCC GGCCAGGGGA AGGAGGAGAA GGAGCCTGGG GTCCTTCGAC TCTCATGACT 
ACGGACTGTC CTGAGCCTTT GCTGCAGCCG TGGGTAGAGA GCTGGAACGT GGAGGGTCCT 
GACAGGCTAC GGGTGAGCTG GTCTCTACCC TCGGTGCCAC TGTCTGGGGA TGGTTTCCTG 
CTGCGTCTGT GGGATGGGGC CCGGGGACAG GAGAGGCGGG AGAACATCTC ATCCCCCCAG 
GCCCGCACTG CCCTCCTGAC TGGACTCACG CCTGGCACGC ACTACCAACT GGATGTGCGG 
CTGTATCACT GCACCCTCCT GGGCCCTGCC TCACCCTCTG CGCATGTGCA CCTGCCCCTC 
AGCGGGCCTC CAGCTCCCCG CCACCTCCGT GCCCAGGCCC TCTCAGACTC TGAGATCCGT 
CTGATGTGGC AGCATCCTGA GGCTCCGCCT GGACCTATAT CCAAGTACAT TGTGGAGATT 
CAGGTGGCCG GGGGCTCAGG AGACCCTCAG TGGATGGATG TAGACAAACC CGAGGAGACG 
AGCACCACCG TCCGTGGTCT CAATGCCAGC ACACGCTACC TCTTCCGTGT GCGGGCCAGT 
GTGCAGGGTC TCGGCGACTG GAGCAACACA GTGGAAGAGA CCACGCTGGG GCTGCAGAGT 
GCGAGCCCAG TCCAAGAGAG CCGGGTGGCT GAAGACGGTC TGGATCAGCA GCTGGTCCTG 
GCTGTGGTAG GCTCCGTCTC TGCCACCTGC CTCACCATCC TGGCTGCCCT TTTAGCCCTG 
GTGTGTATCC GAAGAAGCTG CCTACATCGG AGACATACCT TCACCTACCA GTCAGGATCG 
GGTGAAGAGA CCATCCTGCA GTTCAGCTCA GGGACCTTGA CATTGACCCG ACGGCCAAAG 
CCACAGCCTG AGCCTTTGAG TTACCCTGTG CTGGAGTGGG AGGACATCAC CTTTGAGGAC 
CTCATCGGGG AGGGGAACTT CGGCCAAGTG ATCCGGGCCA TGATCAAGAA GGACGGACTC 
AAGATGAACG CAGCCATCAA GATGCTGAAA GAGTATGCGT CTGAAAATGA CCACCGAGAC 
TTTGCAGGGG AACTAGAAGT TCTGTGCAAA CTAGGACACC ACCCCAATAT TATCAACCTC 
TTGGGGGCCT GTGAGAACCG AGGCTACTTG TATATTGCGA TCGAATATGC CCCGTATGGA 
AACCTGCTAG ATTTCCTGAG AAAGAGCAGG GTCCTGGAGA CTGATCCAGC TTTTGCTCGA 
GAGCATGGAA CAGCCTCCAC ACTCAGCTCC CGGCAGCTGC TGCGCTTTGC CAGTGATGCA 
GCCAACGGGA TGCAGTACCT TAGTGAGAAG CAGTTCATTC ACAGGGACCT GGCTGCCCGA 
AATGTTCTGG TCGGAGAGAA CCTGGCCTCC AAGATTGCAG ACTTTGGCCT TTCTCGGGGG 
GAGGAAGTAT ATGTGAAGAA GACGATGGGC CGTCTTCCTG TGCGTTGGAT GGCCATTGAG 
TCCCTCAACT ACAGCGTCTA CACTACCAAG AGTGACGTCT GGTCATTTGG GGTCCTCCTC 
TGGGAGATAG TGAGCCTTGG AGGCACGCCC TACTGTGGCA TGACCTGTGC TGAGCTCTAC 
GAGAAACTGC CTCAAGGCTA TCGCATGGAG CAGCCTCGAA ACTGTGATGA TGAAGTGTAC 
GAGCTGATGC GGCAGTGCTG GCGGGACCGT CCCTATGAGC GCCCCCCTTT TGCCCAGATT 
GCACTACAGT TGGGCCGCAT GCTGGAAGCC AGGAAGGCCT ACGTGAACAT GTCACTGTTT 
GAGAACTTTA CCTATGCAGG CATCGATGCC ACAGCTGAGG AGGCCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Conditionals by inversion provide a universal method for the generation of conditional alleles.
Proceedings of the National Academy of Sciences of the United States of America110(34)E3179-88(2013 Aug)
Economides AN,Frendewey D,Yang P,Dominguez MG,Dore AT,Lobov IB,Persaud T,Rojas J,McClain J,Lengyel P,Droguett G,Chernomorsky R,Stevens S,Auerbach W,Dechiara TM,Pouyemirou W,Cruz JM Jr,Feeley K,Mellis IA,Yasenchack J,Hatsell SJ,Xie L,Latres E,Huang L,Zhang Y,Pefanis E,Skokos D,Deckelbaum RA,Croll SD,Davis S,Valenzuela DM,Gale NW,Murphy AJ,Yancopoulos GD

Retinoic acid receptor gamma activates receptor tyrosine kinase Tie1 gene transcription through transcription factor GATA4 in F9 stem cells.
Experimental hematology36(5)624-41(2008 May)
Su D,Gudas LJ

Inhibitor of differentiation 1 promotes endothelial survival in a bleomycin model of lung injury in mice.
The American journal of pathology171(4)1113-26(2007 Oct)
Zhang H,Lawson WE,Polosukhin VV,Pozzi A,Blackwell TS,Litingtung Y,Chiang C

Stretch induces upregulation of key tyrosine kinase receptors in microvascular endothelial cells.
American journal of physiology. Heart and circulatory physiology287(6)H2739-45(2004 Dec)
Zheng W,Christensen LP,Tomanek RJ

Enhanced expression of angiopoietin-2 and the Tie2 receptor but not angiopoietin-1 or the Tie1 receptor in a rat model of myocardial infarction.
Journal of biomedical science11(2)163-71(2004 Mar-Apr)
Shyu KG,Liang YJ,Chang H,Wang BW,Leu JG,Kuan P

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Tie1 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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