Cdo1 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Cdo1
Entrez Gene ID	81718
Full Name	cysteine dioxygenase type 1
General protein information	Preferred Names cysteine dioxygenase type 1 Names cysteine dioxygenase type 1 CDO CDO-I cysteine dioxygenase 1, cytosolic cysteine dioxygenase, type I cytosolic cysteine dioxygenase 1
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 18 Map Location: 18q11
Summary	enzyme crucial for the metabolism of cysteine [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
XM_006254698.3	XP_006254760.1	cysteine dioxygenase type 1 isoform X1
NM_052809.1	NP_434696.1	cysteine dioxygenase type 1

Pathways from BioSystems

Homologies

Homo sapiens (human)	CDO1	NP_001792.2
Rattus norvegicus (Norway rat)	Cdo1	NP_434696.1
Gallus gallus (chicken)	CDO1	XP_424964.2
Neurospora crassa	NCU06625	XP_962367.2
Pan troglodytes (chimpanzee)	CDO1	XP_001148883.1
	CDO1	XP_001085951.1
Bos taurus (cattle)	CDO1	NP_001029637.1
Caenorhabditis elegans (roundworm)	cdo-1	NP_508168.1
Xenopus tropicalis (tropical clawed frog)	cdo1	NP_989132.1
Canis lupus familiaris (dog)	CDO1	XP_531867.2
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP005153	XP_314041.4
Magnaporthe oryzae (rice blast fungus)	MGG_06095	XP_003711961.1
Drosophila melanogaster (fruit fly)	CG5493	NP_001246410.1
Mus musculus (house mouse)	Cdo1	NP_149026.1

Gene Ontology
Bibliography

Related articles in PubMed

The Cys-Tyr cross-link of cysteine dioxygenase changes the optimal pH of the reaction without a structural change.
Davies CG, Fellner M, Tchesnokov EP, Wilbanks SM, Jameson GN
Biochemistry53(50)7961-8(2014 Dec)

Shifting redox states of the iron center partitions CDO between crosslink formation or cysteine oxidation.
Njeri CW, Ellis HR
Archives of biochemistry and biophysics55861-9(2014 Sep)

Mechanistic implications of persulfenate and persulfide binding in the active site of cysteine dioxygenase.
Souness RJ, Kleffmann T, Tchesnokov EP, Wilbanks SM, Jameson GB, Jameson GN
Biochemistry52(43)7606-17(2013 Oct)

A strongly bound high-spin iron(II) coordinates cysteine and homocysteine in cysteine dioxygenase.
Tchesnokov EP, Wilbanks SM, Jameson GN
Biochemistry51(1)257-64(2012 Jan)

Correlating crosslink formation with enzymatic activity in cysteine dioxygenase.
Siakkou E, Rutledge MT, Wilbanks SM, Jameson GN
Biochimica et biophysica acta1814(12)2003-9(2011 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

A pH-dependent kinetic study shows that both cross-linked and un-cross-linked CDO are active but the optimal pH decreases with the presence of the cross-link.
Title: The Cys-Tyr cross-link of cysteine dioxygenase changes the optimal pH of the reaction without a structural change.

redox switch commits CDO towards either formation of the Cys93-Tyr157 crosslink or oxidation of the cysteine substrate
Title: Shifting redox states of the iron center partitions CDO between crosslink formation or cysteine oxidation.

Data suggest that biocatalysis by cysteine dioxygenase (CDO1) involves formation of persulfides of both cysteine and 3-mercaptopropionic acid under reducing conditions; these persulfides are stabilized by iron in active site of CDO1.
Title: Mechanistic implications of persulfenate and persulfide binding in the active site of cysteine dioxygenase.

Iron dissociation from the CDO active site exhibits an extremely slow dissociation rate constant, illustrating the stability of the resting state CDO; dioxygenase activity is directly proportional to the concentration of iron.
Title: A strongly bound high-spin iron(II) coordinates cysteine and homocysteine in cysteine dioxygenase.

Correlating crosslink formation with enzymatic activity in cysteine dioxygenase.
Title: Correlating crosslink formation with enzymatic activity in cysteine dioxygenase.

The following Cdo1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Cdo1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa09168	NM_052809.1 Latest version!	Rattus norvegicus cysteine dioxygenase type 1 (Cdo1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$139.30 $199.00
ORa38373	XM_006254698.3 Latest version!	Rattus norvegicus cysteine dioxygenase type 1 (Cdo1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$69.30 $99.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Cdo1 ( NM_052809.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_434696.1 Rattus norvegicus cysteine dioxygenase type 1 (Cdo1), mRNA.

Price & Availability↑

CloneID	ORa09168
Clone ID Related Accession (Same CDS sequence)	NM_052809.1
Accession Version	NM_052809.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 603bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-22
Organism	Rattus norvegicus(Norway rat)
Product	cysteine dioxygenase type 1
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from M35266.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: M35266.1, FQ219057.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMD00052296, SAMD00052297 [ECO:0000350] ##Evidence-Data-END##

ATGGAACGGA CCGAGCTGCT GAAGCCCCGG ACCCTGGCCG ACCTCATCCG AATCTTGCAT 
GAGCTCTTCG CCGGGGACGA AGTCAATGTG GAGGAGGTGC AGGCTGTGCT GGAAGCCTAC 
GAGAGCAATC CTGCCGAGTG GGCTTTGTAT GCCAAATTCG ATCAATACAG GTATACCCGA 
AACCTTGTGG ATCAAGGAAA TGGGAAGTTT AATCTGATGA TTCTGTGCTG GGGTGAAGGG 
CATGGCAGCA GTATTCACGA TCACACGGAC TCCCACTGCT TTTTGAAGCT GCTGCAAGGA 
AATCTAAAGG AGACATTGTT TGACTGGCCT GACAAGAAAT CCAACGAGAT GATCAAGAAG 
TCTGAAAGAA CTTTGAGGGA AAATCAGTGT GCCTACATTA ATGATTCTAT TGGCTTACAT 
CGAGTAGAGA ACGTCAGCCA CACAGAGCCT GCTGTGAGCC TTCACTTGTA CAGTCCACCT 
TTCGATACAT GCCATGCCTT TGACCAACGA ACAGGGCATA AAAACAAAGT CACCATGACA 
TTCCACAGCA AATTTGGAAT CAGAACTCCA TTTACAACTT CAGGTTCACT GGAGAACAAC 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_434696.1
CDS	190..792
Translation	MERTELLKPRTLADLIRILHELFAGDEVNVEEVQAVLEAYESNPAEWALYAKFDQYRYTRNLVDQGNGKFNLMILCWGEGHGSSIHDHTDSHCFLKLLQGNLKETLFDWPDKKSNEMIKKSERTLRENQCAYINDSIGLHRVENVSHTEPAVSLHLYSPPFDTCHAFDQRTGHKNKVTMTFHSKFGIRTPFTTSGSLENN

Target ORF information:

RefSeq Version	NM_052809.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus cysteine dioxygenase type 1 (Cdo1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_052809.1

ORF Insert Sequence:

ATGGAACGGA CCGAGCTGCT GAAGCCCCGG ACCCTGGCCG ACCTCATCCG AATCTTGCAT 
GAGCTCTTCG CCGGGGACGA AGTCAATGTG GAGGAGGTGC AGGCTGTGCT GGAAGCCTAC 
GAGAGCAATC CTGCCGAGTG GGCTTTGTAT GCCAAATTCG ATCAATACAG GTATACCCGA 
AACCTTGTGG ATCAAGGAAA TGGGAAGTTT AATCTGATGA TTCTGTGCTG GGGTGAAGGG 
CATGGCAGCA GTATTCACGA TCACACGGAC TCCCACTGCT TTTTGAAGCT GCTGCAAGGA 
AATCTAAAGG AGACATTGTT TGACTGGCCT GACAAGAAAT CCAACGAGAT GATCAAGAAG 
TCTGAAAGAA CTTTGAGGGA AAATCAGTGT GCCTACATTA ATGATTCTAT TGGCTTACAT 
CGAGTAGAGA ACGTCAGCCA CACAGAGCCT GCTGTGAGCC TTCACTTGTA CAGTCCACCT 
TTCGATACAT GCCATGCCTT TGACCAACGA ACAGGGCATA AAAACAAAGT CACCATGACA 
TTCCACAGCA AATTTGGAAT CAGAACTCCA TTTACAACTT CAGGTTCACT GGAGAACAAC 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Cdo1 ( XM_006254698.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006254760.1 Rattus norvegicus cysteine dioxygenase type 1 (Cdo1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ORa38373
Clone ID Related Accession (Same CDS sequence)	XM_006254698.3
Accession Version	XM_006254698.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 534bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	cysteine dioxygenase type 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005117.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006254698.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAACGGA CCGAGCTGCT GAAGCCCCGG ACCCTGGCCG ACCTCATCCG AATCTTGCAT 
GAGCTCTTCG CCGGGGACGA AGTCAATGTG GAGGAGGTGC AGGCTGTGCT GGAAGCCTAC 
GAGAGCAATC CTGCCGAGTG GGCTTTGTAT GCCAAATTCG ATCAATACAG GTATACTCGA 
AACCTTGTGG ATCAAGGAAA TGGGAAGTTT AATCTGATGA TTCTGTGCTG GGGTGAAGGG 
CATGGCAGCA GTATTCACGA TCACACGGAC TCCCACTGCT TTTTGAAGCT GCTGCAAGGA 
AATCTAAAGG AGACATTGTT TGACTGGCCT GACAAGAAAT CCAACGAGAT GATCAAGAAG 
TCTGAAAGAA CTTTGAGGGA AAATCAGTGT GCCTACATTA ATGACAACTT CAGGTTCACT 
GGAGAACAAC TAAGACCTGC CAAGCCTTTC AAAGTTTTGC TTCTGGGTCG TTGGAATGTT 
TTACCTTGGA TAAGAGAGGC CACCCATCAT TTGCTGTCCA GTTATACATT TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006254760.1
CDS	414..947
Translation	MERTELLKPRTLADLIRILHELFAGDEVNVEEVQAVLEAYESNPAEWALYAKFDQYRYTRNLVDQGNGKFNLMILCWGEGHGSSIHDHTDSHCFLKLLQGNLKETLFDWPDKKSNEMIKKSERTLRENQCAYINDNFRFTGEQLRPAKPFKVLLLGRWNVLPWIREATHHLLSSYTF

Target ORF information:

RefSeq Version	XM_006254698.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus cysteine dioxygenase type 1 (Cdo1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006254698.3

ORF Insert Sequence:

ATGGAACGGA CCGAGCTGCT GAAGCCCCGG ACCCTGGCCG ACCTCATCCG AATCTTGCAT 
GAGCTCTTCG CCGGGGACGA AGTCAATGTG GAGGAGGTGC AGGCTGTGCT GGAAGCCTAC 
GAGAGCAATC CTGCCGAGTG GGCTTTGTAT GCCAAATTCG ATCAATACAG GTATACTCGA 
AACCTTGTGG ATCAAGGAAA TGGGAAGTTT AATCTGATGA TTCTGTGCTG GGGTGAAGGG 
CATGGCAGCA GTATTCACGA TCACACGGAC TCCCACTGCT TTTTGAAGCT GCTGCAAGGA 
AATCTAAAGG AGACATTGTT TGACTGGCCT GACAAGAAAT CCAACGAGAT GATCAAGAAG 
TCTGAAAGAA CTTTGAGGGA AAATCAGTGT GCCTACATTA ATGACAACTT CAGGTTCACT 
GGAGAACAAC TAAGACCTGC CAAGCCTTTC AAAGTTTTGC TTCTGGGTCG TTGGAATGTT 
TTACCTTGGA TAAGAGAGGC CACCCATCAT TTGCTGTCCA GTTATACATT TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The Cys-Tyr cross-link of cysteine dioxygenase changes the optimal pH of the reaction without a structural change.
Biochemistry53(50)7961-8(2014 Dec)
Davies CG,Fellner M,Tchesnokov EP,Wilbanks SM,Jameson GN

Shifting redox states of the iron center partitions CDO between crosslink formation or cysteine oxidation.
Archives of biochemistry and biophysics55861-9(2014 Sep)
Njeri CW,Ellis HR

Mechanistic implications of persulfenate and persulfide binding in the active site of cysteine dioxygenase.
Biochemistry52(43)7606-17(2013 Oct)
Souness RJ,Kleffmann T,Tchesnokov EP,Wilbanks SM,Jameson GB,Jameson GN

A strongly bound high-spin iron(II) coordinates cysteine and homocysteine in cysteine dioxygenase.
Biochemistry51(1)257-64(2012 Jan)
Tchesnokov EP,Wilbanks SM,Jameson GN

Correlating crosslink formation with enzymatic activity in cysteine dioxygenase.
Biochimica et biophysica acta1814(12)2003-9(2011 Dec)
Siakkou E,Rutledge MT,Wilbanks SM,Jameson GN

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Cdo1 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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