Ctbs cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Ctbs
Entrez Gene ID	81652
Full Name	chitobiase
General protein information	Preferred Names chitobiase Names di-N-acetylchitobiase chitobiase, di-N-acetyl-
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 2 Map Location: 2q44
Summary	lysosomal glycosidase involved in the degradation of asparagine-linked glycoproteins [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
NM_031023.1	NP_112285.1	di-N-acetylchitobiase precursor

Pathways from BioSystems

Homologies

Bos taurus (cattle)	CTBS	NP_001193529.1
Xenopus tropicalis (tropical clawed frog)	ctbs	NP_001011500.1
Mus musculus (house mouse)	Ctbs	NP_083112.1
Rattus norvegicus (Norway rat)	Ctbs	NP_112285.1
Gallus gallus (chicken)	CTBS	XP_422372.1
Pan troglodytes (chimpanzee)	CTBS	XP_513520.1
Homo sapiens (human)	CTBS	NP_004379.1
Canis lupus familiaris (dog)	CTBS	XP_547309.2
	CTBS	XP_001107057.1
Danio rerio (zebrafish)	ctbs	NP_001018565.1

Gene Ontology
Bibliography

Related articles in PubMed

New paralogues and revised time line in the expansion of the vertebrate GH18 family.
Hussain M, Wilson JB
Journal of molecular evolution76(4)240-60(2013 Apr)

Human colostrum: identification of minor proteins in the aqueous phase by proteomics.
Palmer DJ, Kelly VC, Smit AM, Kuy S, Knight CG, Cooper GJ
Proteomics6(7)2208-16(2006 Apr)

Cloning and expression of the cDNA sequence encoding the lysosomal glycosidase di-N-acetylchitobiase.
Fisher KJ, Aronson NN Jr
The Journal of biological chemistry267(27)19607-16(1992 Sep)

Rat liver chitobiase: purification, properties, and role in the lysosomal degradation of Asn-linked glycoproteins.
Aronson NN Jr, Backes M, Kuranda MJ
Archives of biochemistry and biophysics272(2)290-300(1989 Aug)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Ctbs gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Ctbs cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa07410	NM_031023.1 Latest version!	Rattus norvegicus chitobiase (Ctbs), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$188.30 $269.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Ctbs ( NM_031023.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_112285.1 Rattus norvegicus chitobiase (Ctbs), mRNA.

Price & Availability↑

CloneID	ORa07410
Clone ID Related Accession (Same CDS sequence)	NM_031023.1
Accession Version	NM_031023.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1104bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-17
Organism	Rattus norvegicus(Norway rat)
Product	di-N-acetylchitobiase precursor
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from M95768.1. ##Evidence-Data-START## Transcript exon combination :: M95768.1, BC088129.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMD00052296, SAMD00052297 [ECO:0000348] ##Evidence-Data-END##

ATGGCGCTGA GTGACCTCCT GGAACTCACT CTGCTGCTGC TGCTGCCCCT GCTGGAGCGG 
CTCTCGGCCG AGGACTGCCC GTGCTCCGAG GCTTCTCTCT GCCGACCGAT CCGCCACCAC 
CGCGACTTCG AGGTCTTTGT GTTTGATGTT GGACAGAAAA CCTGGAAATC TTATGACTGG 
TCGCAGATTA CGACTGTGGC AGTTTTTGGA AAATATGACT CAGAACTTAT GTGCTATGCT 
CATTCGAAAG GAGCCAGGGT AGTGCTAAAA GGTGATGTGG CCCTGAAGGA CATCATTAAT 
CCTACCTTCA GAGCGTCATG GATAGCGCAA AAAGTTGCCT TAGCCAAAGC ACAACACATG 
GATGGAATTA ATATAGACAT AGAACAGGAA GTTGATTGCT CGTCACCTGA ATATGAAGCA 
TTGACAGCTT TGGTCAGAGA AACCACAGAG GGTTTCCATC GTGAGATTGA GGGATCACAG 
GTAACTTTTG ATGTAGCTTG GTCACCAAAG GGCATAGACA AAAGGTGCTA TAATTATACT 
GGGATTGCGG ATGCCTGCGA CTTTCTTTTT GTGATGTCTT ACGATGAGCA AAGTCAGATC 
TGGTCAGAAT GTATTGCAGC CGCCAATGCT CCCTACAATC AGACATTAAC TGGATATGGT 
GACTACCTCA GGATGGGCAT CAGCCCCAGG AAACTTGTAA TGGGTATTCC CTGGTATGGC 
TATGATTACA TTTGCCTAAA CCTCTCAAAG GATGATGTCT GCGCCATTGC AAAGGTCCCT 
TTCAGAGGGG CTCCTTGCAG TGATGCTGCA GGGCATCAGG TGCCCTACAG GGTCATCATG 
AAGCAAGTAA ACAGTTCTGT TTCTGGAAGC CAGTGGAACC AAGACCAGCA AGCTCCGTAC 
TATAATTACA AGGATCCCAC TGGCCGTTTG CATCAGGTGT GGTACGATAA CCCCCGGAGC 
ATCTCACTAA AGGCAGCATT CGTCAAACAC TACGGCTTGC GGGGCATTGG CATGTGGAAC 
GCGAACTGTC TGGACTACTC AGATGACGCT CTAGCCAGAG AGCAAACGGA GGAAATGTGG 
GGAGCCCTGA GACCACGGCT GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_112285.1
CDS	1..1104
Translation	MALSDLLELTLLLLLPLLERLSAEDCPCSEASLCRPIRHHRDFEVFVFDVGQKTWKSYDWSQITTVAVFGKYDSELMCYAHSKGARVVLKGDVALKDIINPTFRASWIAQKVALAKAQHMDGINIDIEQEVDCSSPEYEALTALVRETTEGFHREIEGSQVTFDVAWSPKGIDKRCYNYTGIADACDFLFVMSYDEQSQIWSECIAAANAPYNQTLTGYGDYLRMGISPRKLVMGIPWYGYDYICLNLSKDDVCAIAKVPFRGAPCSDAAGHQVPYRVIMKQVNSSVSGSQWNQDQQAPYYNYKDPTGRLHQVWYDNPRSISLKAAFVKHYGLRGIGMWNANCLDYSDDALAREQTEEMWGALRPRL

Target ORF information:

RefSeq Version	NM_031023.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus chitobiase (Ctbs), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_031023.1

ORF Insert Sequence:

ATGGCGCTGA GTGACCTCCT GGAACTCACT CTGCTGCTGC TGCTGCCCCT GCTGGAGCGG 
CTCTCGGCCG AGGACTGCCC GTGCTCCGAG GCTTCTCTCT GCCGACCGAT CCGCCACCAC 
CGCGACTTCG AGGTCTTTGT GTTTGATGTT GGACAGAAAA CCTGGAAATC TTATGACTGG 
TCGCAGATTA CGACTGTGGC AGTTTTTGGA AAATATGACT CAGAACTTAT GTGCTATGCT 
CATTCGAAAG GAGCCAGGGT AGTGCTAAAA GGTGATGTGG CCCTGAAGGA CATCATTAAT 
CCTACCTTCA GAGCGTCATG GATAGCGCAA AAAGTTGCCT TAGCCAAAGC ACAACACATG 
GATGGAATTA ATATAGACAT AGAACAGGAA GTTGATTGCT CGTCACCTGA ATATGAAGCA 
TTGACAGCTT TGGTCAGAGA AACCACAGAG GGTTTCCATC GTGAGATTGA GGGATCACAG 
GTAACTTTTG ATGTAGCTTG GTCACCAAAG GGCATAGACA AAAGGTGCTA TAATTATACT 
GGGATTGCGG ATGCCTGCGA CTTTCTTTTT GTGATGTCTT ACGATGAGCA AAGTCAGATC 
TGGTCAGAAT GTATTGCAGC CGCCAATGCT CCCTACAATC AGACATTAAC TGGATATGGT 
GACTACCTCA GGATGGGCAT CAGCCCCAGG AAACTTGTAA TGGGTATTCC CTGGTATGGC 
TATGATTACA TTTGCCTAAA CCTCTCAAAG GATGATGTCT GCGCCATTGC AAAGGTCCCT 
TTCAGAGGGG CTCCTTGCAG TGATGCTGCA GGGCATCAGG TGCCCTACAG GGTCATCATG 
AAGCAAGTAA ACAGTTCTGT TTCTGGAAGC CAGTGGAACC AAGACCAGCA AGCTCCGTAC 
TATAATTACA AGGATCCCAC TGGCCGTTTG CATCAGGTGT GGTACGATAA CCCCCGGAGC 
ATCTCACTAA AGGCAGCATT CGTCAAACAC TACGGCTTGC GGGGCATTGG CATGTGGAAC 
GCGAACTGTC TGGACTACTC AGATGACGCT CTAGCCAGAG AGCAAACGGA GGAAATGTGG 
GGAGCCCTGA GACCACGGCT GTGA