Cubn cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Cubn
Entrez Gene ID	80848
Full Name	cubilin
Synonyms	GP280,IFCR,MGA1
General protein information	Preferred Names cubilin Names cubilin 460 kDa receptor glycoprotein 280 intrinsic factor-cobalamin receptor intrinsic factor-vitamin B12 receptor
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 17 Map Location: 17q12.3
Summary	receptor for intrinsic factor (IF)-cobalamin; involved in vitamin B12 uptake (cyanocobalamin) [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
NM_053332.2	NP_445784.1	cubilin precursor

Pathways from BioSystems

Homologies

Mus musculus (house mouse)	Cubn	NP_001074553.1
Canis lupus familiaris (dog)	CUBN	NP_001003148.1
Rattus norvegicus (Norway rat)	Cubn	NP_445784.1
Danio rerio (zebrafish)	cubn	XP_002666724.2
Caenorhabditis elegans (roundworm)	ZC116.3	NP_506157.3
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP005526	XP_315526.4
Bos taurus (cattle)	CUBN	NP_001179504.1
Gallus gallus (chicken)	CUBN	XP_001235156.3
Drosophila melanogaster (fruit fly)	CG32702	NP_727348.2
Homo sapiens (human)	CUBN	NP_001072.2
Xenopus tropicalis (tropical clawed frog)	cubn	XP_002939051.2

Gene Ontology
Bibliography

Related articles in PubMed

Diet induced obesity in rats reduces NHE3 and Na(+) /K(+) -ATPase expression in the kidney.
Briffa JF, Grinfeld E, Jenkin KA, Mathai ML, Poronnik P, McAinch AJ, Hryciw DH
Clinical and experimental pharmacology & physiology42(10)1118-26(2015 Oct)

Megalin/Cubulin-Lysosome-mediated Albumin Reabsorption Is Involved in the Tubular Cell Activation of NLRP3 Inflammasome and Tubulointerstitial Inflammation.
Liu D, Wen Y, Tang TT, Lv LL, Tang RN, Liu H, Ma KL, Crowley SD, Liu BC
The Journal of biological chemistry290(29)18018-28(2015 Jul)

Dab2, megalin, cubilin and amnionless receptor complex might mediate intestinal endocytosis in the suckling rat.
V?zquez-Carretero MD, Palomo M, Garc?a-Miranda P, S?nchez-Aguayo I, Peral MJ, Calonge ML, Ilundain AA
Journal of cellular biochemistry115(3)510-22(2014 Mar)

Impact of lactobacilli on orally acquired listeriosis.
Archambaud C, Nahori MA, Soubigou G, B?cavin C, Laval L, Lechat P, Smokvina T, Langella P, Lecuit M, Cossart P
Proceedings of the National Academy of Sciences of the United States of America109(41)16684-9(2012 Oct)

Cubilin is expressed in rat and human glomerular podocytes.
Prabakaran T, Christensen EI, Nielsen R, Verroust PJ
Nephrology, dialysis, transplantation : official publication of the European Dialysis and Transplant Association - European Renal Association27(8)3156-9(2012 Aug)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The inhibition of megalin/cubilin or lysosomal cathepsin B reduced albuminuria-induced NLRP3 inflammasome activation.
Title: Megalin/Cubulin-Lysosome-mediated Albumin Reabsorption Is Involved in the Tubular Cell Activation of NLRP3 Inflammasome and Tubulointerstitial Inflammation.

We conclude that the megalin/cubilin/amnionless/Dab2 complex/es participate in intestinal processes, mainly during the lactation period and that Dab2 may act as an adaptor in intestinal processes mediated by ApoER2 and VLDLR.
Title: Dab2, megalin, cubilin and amnionless receptor complex might mediate intestinal endocytosis in the suckling rat.

used immunocytochemistry and reverse transcription-polymerase chain reaction on laser-captured glomeruli to demonstrate synthesis and expression of cubilin in glomerular podocytes
Title: Cubilin is expressed in rat and human glomerular podocytes.

cubilin and megalin constitute a dual-receptor complex facilitating the function of each other in the inner ear
Title: Cubilin and megalin co-localize in the neonatal inner ear.

Three distinct regions of cubilin bind to megalin and its interactions with both megalin and amnionless are essential for its intracellular stability.
Title: Interactions of cubilin with megalin and the product of the amnionless gene (AMN): effect on its stability.

The following Cubn gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Cubn cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa09297	NM_053332.2 Latest version!	Rattus norvegicus cubilin (Cubn), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Cubn ( NM_053332.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_445784.1 Rattus norvegicus cubilin (Cubn), mRNA.

Price & Availability↑

CloneID	ORa09297
Clone ID Related Accession (Same CDS sequence)	NM_053332.2
Accession Version	NM_053332.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 10872bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-26
Organism	Rattus norvegicus(Norway rat)
Product	cubilin precursor
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AF022247.1. On Nov 2, 2002 this sequence version replaced NM_053332.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF022247.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMD00052296, SAMD00052297 [ECO:0000350] ##Evidence-Data-END##

ATGTCCTCGC AGTTTCTCTG GGGTTTTGTT ACTTTGTTGA TGATAGCTGA ATTAGATGGC 
AAAACTGGAA AGCCGGAGCA GAGGGGACAG AAAAGGATCG CTGACCTGCA CCAGCCTCGG 
ATGACTACGG AGGAGGGAAA CTTAGTATTT CTTACAAGCT CTACCCAAAA CATTGAATTT 
AGAACTGGAT CCCTGGGGAA AATCAAATTA AATGATGAAG ACCTTGGCGA ATGTTTACAC 
CAGATCCAGA GAAACAAAGA TGATATTATA GATTTAAGAA AGAATACAAC TGGCCTCCCT 
CAAAATATAC TTAGTCAAGT CCACCAACTT AACTCGAAGC TCGTGGATCT GGAGAGAGAT 
TTTCAAAACT TACAGCAGAA TGTGGAGAGA AAAGTTTGCA GTAGCAATCC CTGCCTTAAT 
GGTGGCACCT GCGTCAACCT GCACGACTCC TTTGTGTGTA TCTGCCCTTC TCAGTGGAAG 
GGTCTCTTCT GCTCAGAGGA TGTCAACGAG TGTGTAGTTT ACTCAGGAAC ACCCTTTGGC 
TGCCAGAGTG GATCCACCTG TGTGAACACA GTGGGGAGTT TCAGATGTGA CTGCACGCCG 
GATACGTACG GACCTCAGTG TGCATCCAAA TATAATGACT GTGAACAGGG CTCTAAACAG 
CTCTGCAAGC ACGGCATCTG TGAGGATTTA CAGCGAGTTC ACCATGGACA GCCGAATTTT 
CACTGCATCT GTGATGCTGG GTGGACAACA CCGCCAAATG GAATCTCCTG CACAGAGGAC 
AAAGACGAAT GCAGCCTCCA GCCTTCACCT TGTTCGGAGC ATGCGCAGTG TTTCAATACA 
CAAGGCTCCT TCTACTGTGG GGCCTGCCCC AAAGGTTGGC AGGGAAATGG TTATGAATGC 
CAGGATATCA ATGAATGCGA GATCAACAAT GGAGGCTGTT CCCAGGCGCC ACTGGTCCCA 
TGCCTGAACA CACCTGGATC ATTCTCCTGT GGGAACTGTC CAGCAGGTTT CAGTGGTGAC 
GGGAGAGTGT GCACTCCCGT GGACATCTGT TCGATCCACA ATGGAGGCTG CCATCCAGAG 
GCAACCTGCT CCTCATCTCC TGTTCTAGGG TCCTTCTTGC CTGTCTGCAC CTGTCCTCCG 
GGATATACAG GAAATGGTTA CGGGTCTAAT GGATGTGTAC GCCTCAGTAA TATTTGCTCA 
AGGCACCCGT GTGTAAATGG ACAGTGCATA GAAACAGTAT CAAGTTATTT CTGTAAGTGT 
GACTCAGGCT GGTCTGGACA GAACTGTACA GAAAACATTA ATGACTGTTC GAGCAACCCC 
TGTTTGAATG GGGGCACCTG TATTGATGGC ATCAATGGCT TCACCTGCGA CTGCACAAGC 
TCTTGGACTG GCTATTACTG TCAGACTCCC CAGGCAGCTT GCGGAGGGAT CCTCTCAGGG 
ACACAAGGAA CCTTTGCCTA CCACAGCCCA AATGATACCT ATATTCACAA TGTGAACTGT 
TTCTGGATTG TCAGAACTGA TGAGGAAAAG GTTCTGCATG TCACCTTCAC GTTTTTTGAT 
TTAGAATCAG CAAGCAATTG TCCCCGGGAG TACCTTCAGA TTCATGATGG AGACTCCTCA 
GCGGATTTCC CACTTGGTAG ATACTGTGGC TCCAGGCCCC CCCAGGGGAT CCACAGCAGT 
GCCAATGCTC TCTATTTCCA TCTCTACTCC GAGTACATAA GGAGTGGGAG AGGCTTTACA 
GCAAGGTGGG AGGCGAAGCT GCCAGAGTGT GGCGGCATCC TGACTGATAA TTACGGTTCT 
ATTACGTCTC CGGGGTACCC TGGAAACTAC CCCCCAGGAA GAGATTGTGT CTGGCAGGTT 
TTAGTCAATC CTAACTCCTT GATAACATTT ACCTTTGGAA CCTTGAGCCT GGAGAGCCAC 
AATGACTGCA GCAAAGACTA TTTGGAGATT CGAGACGGTC CTTTCCACCA AGACCCTGTT 
CTTGGGAAAT TCTGCACTTC CTTGTCTACC CCACCCCTCA AGACTACCGG TCCTGCAGCA 
AGAATTCATT TCCATTCTGA CTCTGAGACC AGTGACAAAG GCTTCCACAT CACCTATCTA 
ACCACACAGT CGGATCTGGA CTGTGGTGGG AACTACACAG ACACGGATGG CGAGCTCCTC 
CTTCCTCCTT TGTCTGGTCC TTTCAGTCAC AGCAGACAGT GTGTCTATCT CATCACCCAA 
GCCCAGGGAG AACAAATAGT TATCAACTTC ACCCATGTGG AGCTGGAGAG CCAGATGGGC 
TGTTCTCACA CTTACATCGA GGTTGGAGAC CATGACAGCT TACTTCGAAA GATCTGTGGC 
AATGAAACCT TGTTCCCCAT TAGATCAGTT TCTAATAAAG TCTGGATCAG ATTGAGAATA 
GACGCTTTAG TCCAGAAGGC TAGTTTCAGA GCTGACTACC AAGTTGCTTG TGGGGGTATG 
TTAAGAGGAG AAGGATTCTT TCGCTCACCT TTCTATCCTA ACGCATATCC TGGACGAAGA 
ACCTGTAGGT GGACCATCTC CCAACCCCAA AGACAAGTTG TCCTTCTTAA CTTCACTGAC 
TTTCAGATCG GAAGTTCTGC CTCCTGTGAT ACAGATTATA TTGAGATTGG TCCCAGCTCT 
GTCTTGGGAT CTCCTGGAAA TGAGAAGTTT TGTAGCTCAA ACATACCGTC ATTTATAACA 
TCTGTATACA ATATTCTTTA TGTTACATTT GTGAAAAGTT CTTCCATGGA AAATCGTGGC 
TTCACGGCTA AGTTCAGCAG TGACAAACTA GAGTGTGGAG AAGTTCTCAC GGCATCTACA 
GGAATTATCG AAAGTCCAGG TCATCCAAAC GTCTACCCAA GAGGTGTAAA TTGTACTTGG 
CATGTAGTAG TCCAACGCGG CCAACTGATC CGTTTGGAGT TCAGTTCCTT TTACCTGGAG 
TTTCATTACA ACTGCACAAA CGACTACCTG GAAATTTATG ACACTGCCGC TCAGACTTTT 
CTTGGGAGAT ACTGTGGAAA ATCCATCCCG CCTTCTCTTA CCAGCAACTC TAATTCAATA 
AAGCTGATAT TTGTGTCTGA CTCCGCCCTT GCCCACGAAG GCTTTTCCAT AAATTATGAG 
GCAATCGATG CATCATCAGT ATGTTTATAT GACTATACAG ATAATTTTGG GATGCTCTCC 
TCCCCGAACT TCCCCAATAA TTACCCCAGT AACTGGGAGT GCATCTACAG AATCACTGTG 
GGACTCAACC AACAGATTGC ATTGCATTTC ACAGACTTCA CCTTGGAGGA CTATTTTGGG 
TCACAGTGTG TAGATTTTGT AGAAATCAGA GACGGAGGCT ACGAAACGTC GCCGCTTGTT 
GGGATTTACT GTGGCTCAGT TTTGCCTCCT ACAATCATCT CCCACAGTAA CAAGCTCTGG 
CTAAAGTTTA AGAGTGACGC CGCACTCACG GCAAAGGGGT TCTCAGCGTA CTGGGACGGA 
TCATCAACAG GCTGTGGAGG TAATCTCACC ACCCCCACAG GGGTGCTCAC ATCGCCCAAC 
TACCCGATGC CCTACTACCA CAGCTCCGAA TGCTACTGGC GGCTGGAAGC CAGTCATGGC 
AGCCCTTTCG AGCTGGAATT CCAAGACTTC CACCTGGAAC ACCACCCCAG CTGCTCTCTG 
GATTACTTGG CCGTGTTTGA TGGCCCGACT ACCAACTCCC GACTGATAGA TAAACTGTGT 
GGGGATACGA CACCTGCTCC CATCCGTTCC AATAAAGACG TCGTATTGTT AAAACTGAGG 
ACTGATGCAG GTCAGCAAGG CCGTGGCTTT GAGATCAACT TCCGGCAGAG ATGTGACAAT 
GTGGTGATAG TGAACAAAAC CTCTGGCATC CTGGAGAGCA TAAATTATCC AAATCCCTAT 
GATAAGAACC AACGTTGTAA CTGGACCATC CAAGCAACCA CCGGCAACAC CGTGAACTAC 
ACGTTTCTGG GATTTGATGT GGAAAGTTAC ATGAACTGCT CCACAGATTA TGTAGAGCTC 
TATGATGGAC CACAATGGAT GGGACGCTAC TGTGGAAATA ACATGCCCCC ACCAGGGGCT 
ACAACAGGCT CCCAACTCCA CGTACTGTTC CATACAGATG GGATCAATTC TGGGGAAAAA 
GGATTTAAGA TGCAGTGGTT CACTCATGGC TGTGGTGGAG AGATGTCTGG AACCGCAGGC 
TCCTTCAGCA GCCCTGGGTA CCCCAACAGC TATCCTCACA ACAAAGAGTG TATCTGGAAC 
ATTCGCGTGG CCCCAGGGAG TAGCATTCAG CTCACCATCC ATGACTTTGA TGTGGAATAT 
CATACAAGCT GCAACTATGA CTCCCTGGAG ATCTATGCAG GTCTTGATTT TAACTCTCCA 
AGAATAGCCC AACTGTGTTC CCAATCACCG TCAGCGAACC CCATGCAGGT CTCCAGCACT 
GGCAATGAAC TAGCAATCCG ATTTAAGACG GATAGCACTT TAAATGGAAG AGGTTTCAAT 
GCCTCGTGGC GAGCAGTCCC TGGAGGTTGT GGTGGAATTA TCCAGCTTTC CAGAGGAGAG 
ATTCATTCTC CAAATTACCC CAACAACTAC AGAGCTAACA CAGAGTGCTC CTGGATCATT 
CAAGTTGAGC GACATCACCG TGTTCTCTTG AATATCACTG ACTTTGACCT TGAAGCTCCA 
GATTCTTGCT TACGACTTAT GGATGGCTCA AGTTCCACAA ACGCCCGTGT CGCCAGTGTG 
TGTGGAAGAC AGCAGCCCCC TAACTCTATC ATCGCTTCAG GAAACAGCCT CTTTGTGAGA 
TTCCGGTCTG GATCTTCCAG CCAGAACAGG GGCTTCCGGG CTGAATTCAG GGAAGAGTGC 
GGAGGCCGCA TCATGACCGA CTCTTCCGAT ACTATCTTCT CTCCACTGTA CCCTCACAAC 
TATCTACACA ACCAGAACTG TTCCTGGATA ATTGAAGCTC AGCCTCCATT CAATCACATT 
ACTCTCTCCT TTACTCACTT TCAACTTCAA AACAGCACAG ACTGTACACG GGACTTTGTA 
GAAATTTTGG ATGGCAACGA CTATGACGCA CCTGTCCAAG GCCGTTACTG TGGTTTCTCC 
CTGCCCCACC CCATCATATC ATTTGGCAAT GCCCTAACCG TGAGGTTTGT CACTGATTCC 
ACACGCAGTT TTGAGGGTTT CCGTGCCATC TATTCTGCAT CGACATCATC TTGTGGTGGA 
AGCTTCTACA CACTTGATGG CATCTTCAAT AGCCCCGACT ACCCAGCAGA CTACCATCCA 
AATGCAGAAT GTGTCTGGAA CATTGCCAGC TCCCCTGGCA ACCGCCTGCA ACTGTCCTTC 
CTATCCTTCA ATTTGGAGAA TTCTCTAAAC TGTAACAAGG ATTTTGTGGA AATCCGAGAA 
GGAAATGCCA CGGGCCACTT GATTGGACGA TACTGTGGAA ACTCCCTCCC TGGGAATTAT 
TCGTCAGCTG AGGGACATAG TCTATGGGTC CGATTTGTCT CTGATGGCTC AGGCACTGGC 
ATGGGCTTCC AGGCCAGGTT CAAAAATATA TTTGGCAATA ATAATATTGT GGGAACTCAT 
GGGAAAATCG CATCTCCCTT CTGGCCTGGA AAATACCCCT ACAACTCCAA TTACAAATGG 
GTGGTAAATG TGGACGCATA TCATATTATC CACGGTAGAA TCTTAGAGAT GGACATAGAA 
CCCACAACGA ACTGCTTTTA TGACAGTTTA AAGATTTATG ATGGATTTGA CACTCATTCC 
CGTCTCATTG GCACTTACTG TGGTACCCAG ACAGAATCCT TTAGCTCCAG TAGAAACTCT 
CTGACATTCC AGTTTTCTTC CGACTCTTCT GTGTCAGGAA GGGGATTCCT TCTGGAGTGG 
TTTGCAGTAG ATGTTTCTGA TAGCACCCCT CCCACCATCG CTCCAGGAGC TTGTGGAGGG 
TTTATGGTGA CGGGAGACAC TCCTGTCCAT ATTTTCTCCC CGGGTTGGCC TAGAGAGTAT 
GCTAATGGTG CTGACTGTAT CTGGATCATC TATGCTCCTG ACTCTACTGT GGAACTCAAC 
ATTCTCTCCT TGGACATCGA ACCGCAGCAG TCATGCAATT ATGACAAGCT GATCGTAAAA 
GACGGAGACA GTGACTTATC CCCAGAGCTG GCTGTTCTGT GTGGCGTAAG CCCTCCTGGG 
CCCATCCGGT CAACTGGAGA ATACATGTAC ATCCGCTTCA CTTCAGACAC CAGCGTCGCG 
GGGACAGGCT TCAACGCCTC CTTTCACAAG AGCTGTGGTG GATATTTGCA TGCAGATCGA 
GGAGTTATCA CATCCCCCAA GTATCCAGAC ACCTACCTTC CCAACCTCAA CTGCTCCTGG 
CATGTTCTGG TCCAGACTGG TCTGACCATC GCCGTCCATT TTGAGCAGCC TTTCCAGATT 
CAAAACAGAG ACTCTTTTTG CAGTCAGGGG GATTACTTGG TGCTAAGAAA CGGACCAGAT 
AACCATTCTC CACCACTGGG ACCTTCTGGA AGAAATGGTC GTTTCTGCGG AATGTACGCA 
CCGTCCACTC TGTTCACCTC AGGCAATGAA ATGTTTGTTC AGTTCATCTC GGACAGTAGT 
AATGGTGGAC AAGGGTTTAA GATCAGATAT GAGGCAAAGA GTTTAGCCTG CGGGGGCACT 
GTCTACATCC ATGATGCTGA CTCTGACGGA TACCTGACCT CCCCCAACTA CCCTGCTAAT 
TATCCCCAAC ATGCCGAATG CATTTGGATC TTAGAGGCGC CTCCAGGGAG AAGCATACAG 
CTCCAATTTG AAGATCAATT CAATATTGAA GACACACCCA ACTGTTCTGT GAGCTATCTT 
GAATTGCGTG ACGGAGCCAA CTCGAATGCA CGTCTGGTTT CCAAGTTGTG TGGCCACACT 
CTGCCTCATA GCTGGGTATC CTCGAGAGAA CGAATATACT TGAAGTTTCA CACTGACGGT 
GGTTCCAGCT ACATGGGATT CAAGGCCAAG TACTCTATAG CTTCCTGTGG AGGAACAGTC 
TCAGGGGACA GTGGAGTCAT CGAGAGCATT GGCTACCCGA CCCTTCCGTA TGCAAACAAT 
GTGTTTTGTC AGTGGTTTAT CCGAGGCCTC CCAGGACACT ACCTCACTCT CAGTTTTGAA 
GATTTTAACC TTCAGAGCTC TCCTGGTTGT ACAAAAGACT TTGTGGAGAT CTGGGAAAAC 
CATACCTCTG GAAGAGTTCT GGGGAGATAT TGTGGAAACT CCACTCCTAG CAGTGTTGAC 
ACTTCCAGCA ATGTTGCTTC CGTCAAGTTT GTCACAGATG GCTCTGTCAC TGCCTCAGGA 
TTTAGGCTGC AGTTTAAGTC CAGCAGACAA GTGTGTGGTG GGGATTTACA TGGCCCTACT 
GGCACATTTA CTTCTCCCAA CTACCCAAAC CCAAATCCTC ATGCCCGGAT CTGTGAGTGG 
ACGATCACTG TACAAGAAGG AAGGCGGATC GTCCTGACGT TTACCAACTT GAGGCTGAGT 
ACCCAGCCAT CTTGTAACAG TGAGCACCTC ATCGTATTCA ATGGCATTAG AAGCAACTCG 
CCCCTACTAC AGAAACTGTG CAGCCGTGTG AATGTGACCA ATGAATTCAA ATCTTCAGGA 
AACACCATGA AAGTGGTATT TTTCACTGAT GGCTCCCGGC CGTATGGAGG CTTCACTGCT 
TCCTACACCT CTACTGAAGA TGCAGTGTGT GGTGGATTTC TTCCAAGTGT CTCCGGTGGA 
AACTTTTCTT CTCCTGGCTA TAATGGAATC CGTGATTATG CCAGAAACCT AGACTGTGAA 
TGGACTCTCA GTAATCCAAA TCGGGAAAAT TCATCCATAA GTATCTATTT TCTAGAACTT 
TCCATTGAAA GTCATCAAGA CTGTACATTT GATGTCCTTG AGTTTCGAGT AGGGGATGCT 
GATGGGCCCC TGATAGAGAA GTTCTGTAGC CTGTCAGCAC CAACAGCGCC CTTGGTCATC 
CCCTACCCTC AGGTGTGGAT ACACTTCGTC AGCAATGAGC GTGTAGAATA TACTGGATTC 
TATATAGAGT ACTCCTTTAC AGATTGTGGT GGAATACGGA CAGGTGACAA TGGAGTGATC 
TCAAGTCCTA ACTATCCAAA CTTGTACAGT GCATGGACCC ACTGTTCATG GCTGCTGAAA 
GCCCCAGAAG GGCACACCAT CACTCTCACA TTCAGTGACT TTCTTCTCGA GGCTCATCCA 
ACTTGCACTT CAGACTCCGT CACTGTCAGG AATGGTGACT CCCCAGGATC GCCCGTCATA 
GGACGATACT GTGGACAGTC AGTCCCAAGG CCGATACAGT CTGGTTCCAA CCAACTTATA 
GTGACTTTTA ACACAAACAA TCAAGGGCAA ACTCGTGGAT TTTATGCAAC ATGGACCACA 
AACGCTTTAG GTTGTGGGGG AACATTCCAC TCAGCTAATG GTACAATCAA ATCTCCTCAC 
TGGCCTCAGA CATTCCCAGA AAACAGCAGA TGCTCCTGGA CAGTGATCAC TCACGAAAGT 
AAACACTGGG AGATTAGCTT TGACAGCAAT TTCCGAATCC CCAGCAGTGA CAGCCAGTGT 
CAGAACAGCT TCGTGAAGGT TTGGGAAGGA AGGTTGATGA TCAATAAGAC CCTGTTAGCC 
ACGAGCTGTG GAGATGTGGC TCCAAGTCCC ATTGTCACAT CAGGGAACAT TTTCACTGCT 
GTCTTCCAAT CTGAGGAGAT GGCAGCCCAG GGCTTCTCTG CATCCTTCAT TAGCCGGTGC 
GGACGCACAT TCAATACCTC CCCTGGTGAC ATCATCTCTC CAAACTTCCC GAAGCAATAC 
GACAACAACA TGAACTGCAC CTACCTCATA GACGCTGACC CTCAGTCTCT GGTCATCCTG 
ACTTTTGTGT CCTTTCATTT GGAAGATCGC TCAGCTATCA CCGGAACCTG TGATCATGAT 
GGCTTGCACA TCATCAAAGG TCGTAACCTC TCTTCCACTC CTCTCGTGAC CATATGTGGT 
TCTGAAACTT TGCGTCCCCT CACTGTGGAC GGCCCAGTGT TGCTCAACTT CTATTCTGAT 
GCATACACCA CAGACTTTGG CTTCAAGATT TCCTACAGAG CCATCACCTG TGGTGGGATC 
TACAATGAAT CCTCTGGAAT CCTTAGGAGC CCTTCCTACT CATACAGCAA CTACCCCAAC 
AACCTCTACT GTGTCTACAG CCTCCATGTT AGAAGCAGCA GAGTGATAAT AATTAGGTTC 
AATGATTTCG ATGTGGCTCC TTCCAACCTT TGTGCACATG ACTTCCTGGA GGTGTTTGAT 
GGTCCCAGCA TTGGAAATCG ATCTCTTGGA AAGTTCTGTG GTTCCACGCG TCCACAAACT 
GTTAAGAGCA CCAATAGCAG CCTGACCCTG CTGTTCAAGA CAGATTCTTC TCAAACAGCA 
AGAGGTTGGA AAATATTTTT CCGGGAGACA ATAGGGCCAC AGCAGGGATG TGGTGGATAC 
CTGACCGAGG ACAACCAGAG CTTTGTGTCT CCTGATTCTG ATTCGAATGG ACGCTATGAC 
AAGGGTCTCA GCTGCATATG GTACATAGTT GCACCTGAAA ACAAACTGGT TAAGCTCACC 
TTCAATGTGT TCACTCTGGA GGGACCATCG TCAGCTGGGA GCTGCGTCTA TGATTATGTG 
CAGATAGCAG ATGGCGCAAG CATAAACTCA TATTTAGGTG GAAAATTCTG TGGCTCCCGT 
ATGCCTGCCC CATTTATCTC TTCCGGCAAC TTCCTTACGT TTCAGTTTGT CTCTGACGTA 
ACTGTTGAAA TGAGGGGATT TAATGCAACA TATACCTTTG TGGACATGCC TTGCGGGGGA 
ACATATAACG CAACCTCGAC ACCTCAAAAT GCGTCATCAC CTCATTTATC CAACATCGGA 
CGGCCATACT CCACCTGTAC TTGGGTCATC GCAGCTCCCC CACAGCAGCA GGTTCAGATA 
ACTGTGTGGG ACTTACAGCT GCCCTCACAA GACTGCTCAC AAAGCTACTT AGAACTTCAG 
GATTCAGTAC AGACTGGTGG AAACCGGGTG ACTCAGTTCT GCGGTGCGAA CTATACAACC 
TTGCCAGTGT TCTACTCCTC AATGAGCACT GCGGTTGTCG TTTTCAAGTC TGGAGTTTTA 
AACAGAAACT CGCAAGTGCA ATTCTCCTAT CAGATTGCAG ATTGCAACAG AGAATACAAC 
CAAACGTTTG GCAATCTGAA GAGTCCTGGG TGGCCTCAGA ACTATGACAA TAACCTGGAC 
TGCACCATCA TTCTCAGAGC CCCGCAGAAC CACAGCATTT CCCTCTTTTT CTATTGGTTT 
CAGCTGGAAG ATTCAAGACA ATGCATGAAT GATTTCTTGG AGGTAAGAAA CGGCGGCAGC 
AGCACCTCAC CACTGCTTGA CAAGTACTGT AGCAACCTGC TGCCCAACCC GGTCTTCTCT 
CAGAGCAACG AACTGTATCT GCACTTTCAC AGCGACCACT CAGTCACCAA CAATGGCTAT 
GAAATTATTT GGACCTCCTC TGCTGCTGGC TGTGGAGGGA CTCTTTTGGG CGACGAAGGG 
ATATTCACCA ACCCTGGCTT TCCTGACAGT TACCCGAACA ACACTCATTG TGAATGGACC 
ATTGTTGCTC CTTCTGGAAG GCCTGTCTCT GTCGGGTTTC CCTTTCTCAG TATCGACTCT 
TCTGGTGGCT GTGACCAGAA CTACCTCATA GTCTTTAATG GTCCAGACGC CAACTCCCCA 
CCCTTTGGAC CGTTGTGTGG CATCAACACT GGGATAGCGC CCTTCTATGC TTCATCAAAT 
CGGGTCTTTA TAAGGTTTCA TGCTGAGTAT ACGACACGTC TTTCAGGGTT TGAAATAATG 
TGGAGCAGCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_445784.1
CDS	1..10872
Translation	MSSQFLWGFVTLLMIAELDGKTGKPEQRGQKRIADLHQPRMTTEEGNLVFLTSSTQNIEFRTGSLGKIKLNDEDLGECLHQIQRNKDDIIDLRKNTTGLPQNILSQVHQLNSKLVDLERDFQNLQQNVERKVCSSNPCLNGGTCVNLHDSFVCICPSQWKGLFCSEDVNECVVYSGTPFGCQSGSTCVNTVGSFRCDCTPDTYGPQCASKYNDCEQGSKQLCKHGICEDLQRVHHGQPNFHCICDAGWTTPPNGISCTEDKDECSLQPSPCSEHAQCFNTQGSFYCGACPKGWQGNGYECQDINECEINNGGCSQAPLVPCLNTPGSFSCGNCPAGFSGDGRVCTPVDICSIHNGGCHPEATCSSSPVLGSFLPVCTCPPGYTGNGYGSNGCVRLSNICSRHPCVNGQCIETVSSYFCKCDSGWSGQNCTENINDCSSNPCLNGGTCIDGINGFTCDCTSSWTGYYCQTPQAACGGILSGTQGTFAYHSPNDTYIHNVNCFWIVRTDEEKVLHVTFTFFDLESASNCPREYLQIHDGDSSADFPLGRYCGSRPPQGIHSSANALYFHLYSEYIRSGRGFTARWEAKLPECGGILTDNYGSITSPGYPGNYPPGRDCVWQVLVNPNSLITFTFGTLSLESHNDCSKDYLEIRDGPFHQDPVLGKFCTSLSTPPLKTTGPAARIHFHSDSETSDKGFHITYLTTQSDLDCGGNYTDTDGELLLPPLSGPFSHSRQCVYLITQAQGEQIVINFTHVELESQMGCSHTYIEVGDHDSLLRKICGNETLFPIRSVSNKVWIRLRIDALVQKASFRADYQVACGGMLRGEGFFRSPFYPNAYPGRRTCRWTISQPQRQVVLLNFTDFQIGSSASCDTDYIEIGPSSVLGSPGNEKFCSSNIPSFITSVYNILYVTFVKSSSMENRGFTAKFSSDKLECGEVLTASTGIIESPGHPNVYPRGVNCTWHVVVQRGQLIRLEFSSFYLEFHYNCTNDYLEIYDTAAQTFLGRYCGKSIPPSLTSNSNSIKLIFVSDSALAHEGFSINYEAIDASSVCLYDYTDNFGMLSSPNFPNNYPSNWECIYRITVGLNQQIALHFTDFTLEDYFGSQCVDFVEIRDGGYETSPLVGIYCGSVLPPTIISHSNKLWLKFKSDAALTAKGFSAYWDGSSTGCGGNLTTPTGVLTSPNYPMPYYHSSECYWRLEASHGSPFELEFQDFHLEHHPSCSLDYLAVFDGPTTNSRLIDKLCGDTTPAPIRSNKDVVLLKLRTDAGQQGRGFEINFRQRCDNVVIVNKTSGILESINYPNPYDKNQRCNWTIQATTGNTVNYTFLGFDVESYMNCSTDYVELYDGPQWMGRYCGNNMPPPGATTGSQLHVLFHTDGINSGEKGFKMQWFTHGCGGEMSGTAGSFSSPGYPNSYPHNKECIWNIRVAPGSSIQLTIHDFDVEYHTSCNYDSLEIYAGLDFNSPRIAQLCSQSPSANPMQVSSTGNELAIRFKTDSTLNGRGFNASWRAVPGGCGGIIQLSRGEIHSPNYPNNYRANTECSWIIQVERHHRVLLNITDFDLEAPDSCLRLMDGSSSTNARVASVCGRQQPPNSIIASGNSLFVRFRSGSSSQNRGFRAEFREECGGRIMTDSSDTIFSPLYPHNYLHNQNCSWIIEAQPPFNHITLSFTHFQLQNSTDCTRDFVEILDGNDYDAPVQGRYCGFSLPHPIISFGNALTVRFVTDSTRSFEGFRAIYSASTSSCGGSFYTLDGIFNSPDYPADYHPNAECVWNIASSPGNRLQLSFLSFNLENSLNCNKDFVEIREGNATGHLIGRYCGNSLPGNYSSAEGHSLWVRFVSDGSGTGMGFQARFKNIFGNNNIVGTHGKIASPFWPGKYPYNSNYKWVVNVDAYHIIHGRILEMDIEPTTNCFYDSLKIYDGFDTHSRLIGTYCGTQTESFSSSRNSLTFQFSSDSSVSGRGFLLEWFAVDVSDSTPPTIAPGACGGFMVTGDTPVHIFSPGWPREYANGADCIWIIYAPDSTVELNILSLDIEPQQSCNYDKLIVKDGDSDLSPELAVLCGVSPPGPIRSTGEYMYIRFTSDTSVAGTGFNASFHKSCGGYLHADRGVITSPKYPDTYLPNLNCSWHVLVQTGLTIAVHFEQPFQIQNRDSFCSQGDYLVLRNGPDNHSPPLGPSGRNGRFCGMYAPSTLFTSGNEMFVQFISDSSNGGQGFKIRYEAKSLACGGTVYIHDADSDGYLTSPNYPANYPQHAECIWILEAPPGRSIQLQFEDQFNIEDTPNCSVSYLELRDGANSNARLVSKLCGHTLPHSWVSSRERIYLKFHTDGGSSYMGFKAKYSIASCGGTVSGDSGVIESIGYPTLPYANNVFCQWFIRGLPGHYLTLSFEDFNLQSSPGCTKDFVEIWENHTSGRVLGRYCGNSTPSSVDTSSNVASVKFVTDGSVTASGFRLQFKSSRQVCGGDLHGPTGTFTSPNYPNPNPHARICEWTITVQEGRRIVLTFTNLRLSTQPSCNSEHLIVFNGIRSNSPLLQKLCSRVNVTNEFKSSGNTMKVVFFTDGSRPYGGFTASYTSTEDAVCGGFLPSVSGGNFSSPGYNGIRDYARNLDCEWTLSNPNRENSSISIYFLELSIESHQDCTFDVLEFRVGDADGPLIEKFCSLSAPTAPLVIPYPQVWIHFVSNERVEYTGFYIEYSFTDCGGIRTGDNGVISSPNYPNLYSAWTHCSWLLKAPEGHTITLTFSDFLLEAHPTCTSDSVTVRNGDSPGSPVIGRYCGQSVPRPIQSGSNQLIVTFNTNNQGQTRGFYATWTTNALGCGGTFHSANGTIKSPHWPQTFPENSRCSWTVITHESKHWEISFDSNFRIPSSDSQCQNSFVKVWEGRLMINKTLLATSCGDVAPSPIVTSGNIFTAVFQSEEMAAQGFSASFISRCGRTFNTSPGDIISPNFPKQYDNNMNCTYLIDADPQSLVILTFVSFHLEDRSAITGTCDHDGLHIIKGRNLSSTPLVTICGSETLRPLTVDGPVLLNFYSDAYTTDFGFKISYRAITCGGIYNESSGILRSPSYSYSNYPNNLYCVYSLHVRSSRVIIIRFNDFDVAPSNLCAHDFLEVFDGPSIGNRSLGKFCGSTRPQTVKSTNSSLTLLFKTDSSQTARGWKIFFRETIGPQQGCGGYLTEDNQSFVSPDSDSNGRYDKGLSCIWYIVAPENKLVKLTFNVFTLEGPSSAGSCVYDYVQIADGASINSYLGGKFCGSRMPAPFISSGNFLTFQFVSDVTVEMRGFNATYTFVDMPCGGTYNATSTPQNASSPHLSNIGRPYSTCTWVIAAPPQQQVQITVWDLQLPSQDCSQSYLELQDSVQTGGNRVTQFCGANYTTLPVFYSSMSTAVVVFKSGVLNRNSQVQFSYQIADCNREYNQTFGNLKSPGWPQNYDNNLDCTIILRAPQNHSISLFFYWFQLEDSRQCMNDFLEVRNGGSSTSPLLDKYCSNLLPNPVFSQSNELYLHFHSDHSVTNNGYEIIWTSSAAGCGGTLLGDEGIFTNPGFPDSYPNNTHCEWTIVAPSGRPVSVGFPFLSIDSSGGCDQNYLIVFNGPDANSPPFGPLCGINTGIAPFYASSNRVFIRFHAEYTTRLSGFEIMWSS

Target ORF information:

RefSeq Version	NM_053332.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus cubilin (Cubn), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_053332.2

ORF Insert Sequence:

ATGTCCTCGC AGTTTCTCTG GGGTTTTGTT ACTTTGTTGA TGATAGCTGA ATTAGATGGC 
AAAACTGGAA AGCCGGAGCA GAGGGGACAG AAAAGGATCG CTGACCTGCA CCAGCCTCGG 
ATGACTACGG AGGAGGGAAA CTTAGTATTT CTTACAAGCT CTACCCAAAA CATTGAATTT 
AGAACTGGAT CCCTGGGGAA AATCAAATTA AATGATGAAG ACCTTGGCGA ATGTTTACAC 
CAGATCCAGA GAAACAAAGA TGATATTATA GATTTAAGAA AGAATACAAC TGGCCTCCCT 
CAAAATATAC TTAGTCAAGT CCACCAACTT AACTCGAAGC TCGTGGATCT GGAGAGAGAT 
TTTCAAAACT TACAGCAGAA TGTGGAGAGA AAAGTTTGCA GTAGCAATCC CTGCCTTAAT 
GGTGGCACCT GCGTCAACCT GCACGACTCC TTTGTGTGTA TCTGCCCTTC TCAGTGGAAG 
GGTCTCTTCT GCTCAGAGGA TGTCAACGAG TGTGTAGTTT ACTCAGGAAC ACCCTTTGGC 
TGCCAGAGTG GATCCACCTG TGTGAACACA GTGGGGAGTT TCAGATGTGA CTGCACGCCG 
GATACGTACG GACCTCAGTG TGCATCCAAA TATAATGACT GTGAACAGGG CTCTAAACAG 
CTCTGCAAGC ACGGCATCTG TGAGGATTTA CAGCGAGTTC ACCATGGACA GCCGAATTTT 
CACTGCATCT GTGATGCTGG GTGGACAACA CCGCCAAATG GAATCTCCTG CACAGAGGAC 
AAAGACGAAT GCAGCCTCCA GCCTTCACCT TGTTCGGAGC ATGCGCAGTG TTTCAATACA 
CAAGGCTCCT TCTACTGTGG GGCCTGCCCC AAAGGTTGGC AGGGAAATGG TTATGAATGC 
CAGGATATCA ATGAATGCGA GATCAACAAT GGAGGCTGTT CCCAGGCGCC ACTGGTCCCA 
TGCCTGAACA CACCTGGATC ATTCTCCTGT GGGAACTGTC CAGCAGGTTT CAGTGGTGAC 
GGGAGAGTGT GCACTCCCGT GGACATCTGT TCGATCCACA ATGGAGGCTG CCATCCAGAG 
GCAACCTGCT CCTCATCTCC TGTTCTAGGG TCCTTCTTGC CTGTCTGCAC CTGTCCTCCG 
GGATATACAG GAAATGGTTA CGGGTCTAAT GGATGTGTAC GCCTCAGTAA TATTTGCTCA 
AGGCACCCGT GTGTAAATGG ACAGTGCATA GAAACAGTAT CAAGTTATTT CTGTAAGTGT 
GACTCAGGCT GGTCTGGACA GAACTGTACA GAAAACATTA ATGACTGTTC GAGCAACCCC 
TGTTTGAATG GGGGCACCTG TATTGATGGC ATCAATGGCT TCACCTGCGA CTGCACAAGC 
TCTTGGACTG GCTATTACTG TCAGACTCCC CAGGCAGCTT GCGGAGGGAT CCTCTCAGGG 
ACACAAGGAA CCTTTGCCTA CCACAGCCCA AATGATACCT ATATTCACAA TGTGAACTGT 
TTCTGGATTG TCAGAACTGA TGAGGAAAAG GTTCTGCATG TCACCTTCAC GTTTTTTGAT 
TTAGAATCAG CAAGCAATTG TCCCCGGGAG TACCTTCAGA TTCATGATGG AGACTCCTCA 
GCGGATTTCC CACTTGGTAG ATACTGTGGC TCCAGGCCCC CCCAGGGGAT CCACAGCAGT 
GCCAATGCTC TCTATTTCCA TCTCTACTCC GAGTACATAA GGAGTGGGAG AGGCTTTACA 
GCAAGGTGGG AGGCGAAGCT GCCAGAGTGT GGCGGCATCC TGACTGATAA TTACGGTTCT 
ATTACGTCTC CGGGGTACCC TGGAAACTAC CCCCCAGGAA GAGATTGTGT CTGGCAGGTT 
TTAGTCAATC CTAACTCCTT GATAACATTT ACCTTTGGAA CCTTGAGCCT GGAGAGCCAC 
AATGACTGCA GCAAAGACTA TTTGGAGATT CGAGACGGTC CTTTCCACCA AGACCCTGTT 
CTTGGGAAAT TCTGCACTTC CTTGTCTACC CCACCCCTCA AGACTACCGG TCCTGCAGCA 
AGAATTCATT TCCATTCTGA CTCTGAGACC AGTGACAAAG GCTTCCACAT CACCTATCTA 
ACCACACAGT CGGATCTGGA CTGTGGTGGG AACTACACAG ACACGGATGG CGAGCTCCTC 
CTTCCTCCTT TGTCTGGTCC TTTCAGTCAC AGCAGACAGT GTGTCTATCT CATCACCCAA 
GCCCAGGGAG AACAAATAGT TATCAACTTC ACCCATGTGG AGCTGGAGAG CCAGATGGGC 
TGTTCTCACA CTTACATCGA GGTTGGAGAC CATGACAGCT TACTTCGAAA GATCTGTGGC 
AATGAAACCT TGTTCCCCAT TAGATCAGTT TCTAATAAAG TCTGGATCAG ATTGAGAATA 
GACGCTTTAG TCCAGAAGGC TAGTTTCAGA GCTGACTACC AAGTTGCTTG TGGGGGTATG 
TTAAGAGGAG AAGGATTCTT TCGCTCACCT TTCTATCCTA ACGCATATCC TGGACGAAGA 
ACCTGTAGGT GGACCATCTC CCAACCCCAA AGACAAGTTG TCCTTCTTAA CTTCACTGAC 
TTTCAGATCG GAAGTTCTGC CTCCTGTGAT ACAGATTATA TTGAGATTGG TCCCAGCTCT 
GTCTTGGGAT CTCCTGGAAA TGAGAAGTTT TGTAGCTCAA ACATACCGTC ATTTATAACA 
TCTGTATACA ATATTCTTTA TGTTACATTT GTGAAAAGTT CTTCCATGGA AAATCGTGGC 
TTCACGGCTA AGTTCAGCAG TGACAAACTA GAGTGTGGAG AAGTTCTCAC GGCATCTACA 
GGAATTATCG AAAGTCCAGG TCATCCAAAC GTCTACCCAA GAGGTGTAAA TTGTACTTGG 
CATGTAGTAG TCCAACGCGG CCAACTGATC CGTTTGGAGT TCAGTTCCTT TTACCTGGAG 
TTTCATTACA ACTGCACAAA CGACTACCTG GAAATTTATG ACACTGCCGC TCAGACTTTT 
CTTGGGAGAT ACTGTGGAAA ATCCATCCCG CCTTCTCTTA CCAGCAACTC TAATTCAATA 
AAGCTGATAT TTGTGTCTGA CTCCGCCCTT GCCCACGAAG GCTTTTCCAT AAATTATGAG 
GCAATCGATG CATCATCAGT ATGTTTATAT GACTATACAG ATAATTTTGG GATGCTCTCC 
TCCCCGAACT TCCCCAATAA TTACCCCAGT AACTGGGAGT GCATCTACAG AATCACTGTG 
GGACTCAACC AACAGATTGC ATTGCATTTC ACAGACTTCA CCTTGGAGGA CTATTTTGGG 
TCACAGTGTG TAGATTTTGT AGAAATCAGA GACGGAGGCT ACGAAACGTC GCCGCTTGTT 
GGGATTTACT GTGGCTCAGT TTTGCCTCCT ACAATCATCT CCCACAGTAA CAAGCTCTGG 
CTAAAGTTTA AGAGTGACGC CGCACTCACG GCAAAGGGGT TCTCAGCGTA CTGGGACGGA 
TCATCAACAG GCTGTGGAGG TAATCTCACC ACCCCCACAG GGGTGCTCAC ATCGCCCAAC 
TACCCGATGC CCTACTACCA CAGCTCCGAA TGCTACTGGC GGCTGGAAGC CAGTCATGGC 
AGCCCTTTCG AGCTGGAATT CCAAGACTTC CACCTGGAAC ACCACCCCAG CTGCTCTCTG 
GATTACTTGG CCGTGTTTGA TGGCCCGACT ACCAACTCCC GACTGATAGA TAAACTGTGT 
GGGGATACGA CACCTGCTCC CATCCGTTCC AATAAAGACG TCGTATTGTT AAAACTGAGG 
ACTGATGCAG GTCAGCAAGG CCGTGGCTTT GAGATCAACT TCCGGCAGAG ATGTGACAAT 
GTGGTGATAG TGAACAAAAC CTCTGGCATC CTGGAGAGCA TAAATTATCC AAATCCCTAT 
GATAAGAACC AACGTTGTAA CTGGACCATC CAAGCAACCA CCGGCAACAC CGTGAACTAC 
ACGTTTCTGG GATTTGATGT GGAAAGTTAC ATGAACTGCT CCACAGATTA TGTAGAGCTC 
TATGATGGAC CACAATGGAT GGGACGCTAC TGTGGAAATA ACATGCCCCC ACCAGGGGCT 
ACAACAGGCT CCCAACTCCA CGTACTGTTC CATACAGATG GGATCAATTC TGGGGAAAAA 
GGATTTAAGA TGCAGTGGTT CACTCATGGC TGTGGTGGAG AGATGTCTGG AACCGCAGGC 
TCCTTCAGCA GCCCTGGGTA CCCCAACAGC TATCCTCACA ACAAAGAGTG TATCTGGAAC 
ATTCGCGTGG CCCCAGGGAG TAGCATTCAG CTCACCATCC ATGACTTTGA TGTGGAATAT 
CATACAAGCT GCAACTATGA CTCCCTGGAG ATCTATGCAG GTCTTGATTT TAACTCTCCA 
AGAATAGCCC AACTGTGTTC CCAATCACCG TCAGCGAACC CCATGCAGGT CTCCAGCACT 
GGCAATGAAC TAGCAATCCG ATTTAAGACG GATAGCACTT TAAATGGAAG AGGTTTCAAT 
GCCTCGTGGC GAGCAGTCCC TGGAGGTTGT GGTGGAATTA TCCAGCTTTC CAGAGGAGAG 
ATTCATTCTC CAAATTACCC CAACAACTAC AGAGCTAACA CAGAGTGCTC CTGGATCATT 
CAAGTTGAGC GACATCACCG TGTTCTCTTG AATATCACTG ACTTTGACCT TGAAGCTCCA 
GATTCTTGCT TACGACTTAT GGATGGCTCA AGTTCCACAA ACGCCCGTGT CGCCAGTGTG 
TGTGGAAGAC AGCAGCCCCC TAACTCTATC ATCGCTTCAG GAAACAGCCT CTTTGTGAGA 
TTCCGGTCTG GATCTTCCAG CCAGAACAGG GGCTTCCGGG CTGAATTCAG GGAAGAGTGC 
GGAGGCCGCA TCATGACCGA CTCTTCCGAT ACTATCTTCT CTCCACTGTA CCCTCACAAC 
TATCTACACA ACCAGAACTG TTCCTGGATA ATTGAAGCTC AGCCTCCATT CAATCACATT 
ACTCTCTCCT TTACTCACTT TCAACTTCAA AACAGCACAG ACTGTACACG GGACTTTGTA 
GAAATTTTGG ATGGCAACGA CTATGACGCA CCTGTCCAAG GCCGTTACTG TGGTTTCTCC 
CTGCCCCACC CCATCATATC ATTTGGCAAT GCCCTAACCG TGAGGTTTGT CACTGATTCC 
ACACGCAGTT TTGAGGGTTT CCGTGCCATC TATTCTGCAT CGACATCATC TTGTGGTGGA 
AGCTTCTACA CACTTGATGG CATCTTCAAT AGCCCCGACT ACCCAGCAGA CTACCATCCA 
AATGCAGAAT GTGTCTGGAA CATTGCCAGC TCCCCTGGCA ACCGCCTGCA ACTGTCCTTC 
CTATCCTTCA ATTTGGAGAA TTCTCTAAAC TGTAACAAGG ATTTTGTGGA AATCCGAGAA 
GGAAATGCCA CGGGCCACTT GATTGGACGA TACTGTGGAA ACTCCCTCCC TGGGAATTAT 
TCGTCAGCTG AGGGACATAG TCTATGGGTC CGATTTGTCT CTGATGGCTC AGGCACTGGC 
ATGGGCTTCC AGGCCAGGTT CAAAAATATA TTTGGCAATA ATAATATTGT GGGAACTCAT 
GGGAAAATCG CATCTCCCTT CTGGCCTGGA AAATACCCCT ACAACTCCAA TTACAAATGG 
GTGGTAAATG TGGACGCATA TCATATTATC CACGGTAGAA TCTTAGAGAT GGACATAGAA 
CCCACAACGA ACTGCTTTTA TGACAGTTTA AAGATTTATG ATGGATTTGA CACTCATTCC 
CGTCTCATTG GCACTTACTG TGGTACCCAG ACAGAATCCT TTAGCTCCAG TAGAAACTCT 
CTGACATTCC AGTTTTCTTC CGACTCTTCT GTGTCAGGAA GGGGATTCCT TCTGGAGTGG 
TTTGCAGTAG ATGTTTCTGA TAGCACCCCT CCCACCATCG CTCCAGGAGC TTGTGGAGGG 
TTTATGGTGA CGGGAGACAC TCCTGTCCAT ATTTTCTCCC CGGGTTGGCC TAGAGAGTAT 
GCTAATGGTG CTGACTGTAT CTGGATCATC TATGCTCCTG ACTCTACTGT GGAACTCAAC 
ATTCTCTCCT TGGACATCGA ACCGCAGCAG TCATGCAATT ATGACAAGCT GATCGTAAAA 
GACGGAGACA GTGACTTATC CCCAGAGCTG GCTGTTCTGT GTGGCGTAAG CCCTCCTGGG 
CCCATCCGGT CAACTGGAGA ATACATGTAC ATCCGCTTCA CTTCAGACAC CAGCGTCGCG 
GGGACAGGCT TCAACGCCTC CTTTCACAAG AGCTGTGGTG GATATTTGCA TGCAGATCGA 
GGAGTTATCA CATCCCCCAA GTATCCAGAC ACCTACCTTC CCAACCTCAA CTGCTCCTGG 
CATGTTCTGG TCCAGACTGG TCTGACCATC GCCGTCCATT TTGAGCAGCC TTTCCAGATT 
CAAAACAGAG ACTCTTTTTG CAGTCAGGGG GATTACTTGG TGCTAAGAAA CGGACCAGAT 
AACCATTCTC CACCACTGGG ACCTTCTGGA AGAAATGGTC GTTTCTGCGG AATGTACGCA 
CCGTCCACTC TGTTCACCTC AGGCAATGAA ATGTTTGTTC AGTTCATCTC GGACAGTAGT 
AATGGTGGAC AAGGGTTTAA GATCAGATAT GAGGCAAAGA GTTTAGCCTG CGGGGGCACT 
GTCTACATCC ATGATGCTGA CTCTGACGGA TACCTGACCT CCCCCAACTA CCCTGCTAAT 
TATCCCCAAC ATGCCGAATG CATTTGGATC TTAGAGGCGC CTCCAGGGAG AAGCATACAG 
CTCCAATTTG AAGATCAATT CAATATTGAA GACACACCCA ACTGTTCTGT GAGCTATCTT 
GAATTGCGTG ACGGAGCCAA CTCGAATGCA CGTCTGGTTT CCAAGTTGTG TGGCCACACT 
CTGCCTCATA GCTGGGTATC CTCGAGAGAA CGAATATACT TGAAGTTTCA CACTGACGGT 
GGTTCCAGCT ACATGGGATT CAAGGCCAAG TACTCTATAG CTTCCTGTGG AGGAACAGTC 
TCAGGGGACA GTGGAGTCAT CGAGAGCATT GGCTACCCGA CCCTTCCGTA TGCAAACAAT 
GTGTTTTGTC AGTGGTTTAT CCGAGGCCTC CCAGGACACT ACCTCACTCT CAGTTTTGAA 
GATTTTAACC TTCAGAGCTC TCCTGGTTGT ACAAAAGACT TTGTGGAGAT CTGGGAAAAC 
CATACCTCTG GAAGAGTTCT GGGGAGATAT TGTGGAAACT CCACTCCTAG CAGTGTTGAC 
ACTTCCAGCA ATGTTGCTTC CGTCAAGTTT GTCACAGATG GCTCTGTCAC TGCCTCAGGA 
TTTAGGCTGC AGTTTAAGTC CAGCAGACAA GTGTGTGGTG GGGATTTACA TGGCCCTACT 
GGCACATTTA CTTCTCCCAA CTACCCAAAC CCAAATCCTC ATGCCCGGAT CTGTGAGTGG 
ACGATCACTG TACAAGAAGG AAGGCGGATC GTCCTGACGT TTACCAACTT GAGGCTGAGT 
ACCCAGCCAT CTTGTAACAG TGAGCACCTC ATCGTATTCA ATGGCATTAG AAGCAACTCG 
CCCCTACTAC AGAAACTGTG CAGCCGTGTG AATGTGACCA ATGAATTCAA ATCTTCAGGA 
AACACCATGA AAGTGGTATT TTTCACTGAT GGCTCCCGGC CGTATGGAGG CTTCACTGCT 
TCCTACACCT CTACTGAAGA TGCAGTGTGT GGTGGATTTC TTCCAAGTGT CTCCGGTGGA 
AACTTTTCTT CTCCTGGCTA TAATGGAATC CGTGATTATG CCAGAAACCT AGACTGTGAA 
TGGACTCTCA GTAATCCAAA TCGGGAAAAT TCATCCATAA GTATCTATTT TCTAGAACTT 
TCCATTGAAA GTCATCAAGA CTGTACATTT GATGTCCTTG AGTTTCGAGT AGGGGATGCT 
GATGGGCCCC TGATAGAGAA GTTCTGTAGC CTGTCAGCAC CAACAGCGCC CTTGGTCATC 
CCCTACCCTC AGGTGTGGAT ACACTTCGTC AGCAATGAGC GTGTAGAATA TACTGGATTC 
TATATAGAGT ACTCCTTTAC AGATTGTGGT GGAATACGGA CAGGTGACAA TGGAGTGATC 
TCAAGTCCTA ACTATCCAAA CTTGTACAGT GCATGGACCC ACTGTTCATG GCTGCTGAAA 
GCCCCAGAAG GGCACACCAT CACTCTCACA TTCAGTGACT TTCTTCTCGA GGCTCATCCA 
ACTTGCACTT CAGACTCCGT CACTGTCAGG AATGGTGACT CCCCAGGATC GCCCGTCATA 
GGACGATACT GTGGACAGTC AGTCCCAAGG CCGATACAGT CTGGTTCCAA CCAACTTATA 
GTGACTTTTA ACACAAACAA TCAAGGGCAA ACTCGTGGAT TTTATGCAAC ATGGACCACA 
AACGCTTTAG GTTGTGGGGG AACATTCCAC TCAGCTAATG GTACAATCAA ATCTCCTCAC 
TGGCCTCAGA CATTCCCAGA AAACAGCAGA TGCTCCTGGA CAGTGATCAC TCACGAAAGT 
AAACACTGGG AGATTAGCTT TGACAGCAAT TTCCGAATCC CCAGCAGTGA CAGCCAGTGT 
CAGAACAGCT TCGTGAAGGT TTGGGAAGGA AGGTTGATGA TCAATAAGAC CCTGTTAGCC 
ACGAGCTGTG GAGATGTGGC TCCAAGTCCC ATTGTCACAT CAGGGAACAT TTTCACTGCT 
GTCTTCCAAT CTGAGGAGAT GGCAGCCCAG GGCTTCTCTG CATCCTTCAT TAGCCGGTGC 
GGACGCACAT TCAATACCTC CCCTGGTGAC ATCATCTCTC CAAACTTCCC GAAGCAATAC 
GACAACAACA TGAACTGCAC CTACCTCATA GACGCTGACC CTCAGTCTCT GGTCATCCTG 
ACTTTTGTGT CCTTTCATTT GGAAGATCGC TCAGCTATCA CCGGAACCTG TGATCATGAT 
GGCTTGCACA TCATCAAAGG TCGTAACCTC TCTTCCACTC CTCTCGTGAC CATATGTGGT 
TCTGAAACTT TGCGTCCCCT CACTGTGGAC GGCCCAGTGT TGCTCAACTT CTATTCTGAT 
GCATACACCA CAGACTTTGG CTTCAAGATT TCCTACAGAG CCATCACCTG TGGTGGGATC 
TACAATGAAT CCTCTGGAAT CCTTAGGAGC CCTTCCTACT CATACAGCAA CTACCCCAAC 
AACCTCTACT GTGTCTACAG CCTCCATGTT AGAAGCAGCA GAGTGATAAT AATTAGGTTC 
AATGATTTCG ATGTGGCTCC TTCCAACCTT TGTGCACATG ACTTCCTGGA GGTGTTTGAT 
GGTCCCAGCA TTGGAAATCG ATCTCTTGGA AAGTTCTGTG GTTCCACGCG TCCACAAACT 
GTTAAGAGCA CCAATAGCAG CCTGACCCTG CTGTTCAAGA CAGATTCTTC TCAAACAGCA 
AGAGGTTGGA AAATATTTTT CCGGGAGACA ATAGGGCCAC AGCAGGGATG TGGTGGATAC 
CTGACCGAGG ACAACCAGAG CTTTGTGTCT CCTGATTCTG ATTCGAATGG ACGCTATGAC 
AAGGGTCTCA GCTGCATATG GTACATAGTT GCACCTGAAA ACAAACTGGT TAAGCTCACC 
TTCAATGTGT TCACTCTGGA GGGACCATCG TCAGCTGGGA GCTGCGTCTA TGATTATGTG 
CAGATAGCAG ATGGCGCAAG CATAAACTCA TATTTAGGTG GAAAATTCTG TGGCTCCCGT 
ATGCCTGCCC CATTTATCTC TTCCGGCAAC TTCCTTACGT TTCAGTTTGT CTCTGACGTA 
ACTGTTGAAA TGAGGGGATT TAATGCAACA TATACCTTTG TGGACATGCC TTGCGGGGGA 
ACATATAACG CAACCTCGAC ACCTCAAAAT GCGTCATCAC CTCATTTATC CAACATCGGA 
CGGCCATACT CCACCTGTAC TTGGGTCATC GCAGCTCCCC CACAGCAGCA GGTTCAGATA 
ACTGTGTGGG ACTTACAGCT GCCCTCACAA GACTGCTCAC AAAGCTACTT AGAACTTCAG 
GATTCAGTAC AGACTGGTGG AAACCGGGTG ACTCAGTTCT GCGGTGCGAA CTATACAACC 
TTGCCAGTGT TCTACTCCTC AATGAGCACT GCGGTTGTCG TTTTCAAGTC TGGAGTTTTA 
AACAGAAACT CGCAAGTGCA ATTCTCCTAT CAGATTGCAG ATTGCAACAG AGAATACAAC 
CAAACGTTTG GCAATCTGAA GAGTCCTGGG TGGCCTCAGA ACTATGACAA TAACCTGGAC 
TGCACCATCA TTCTCAGAGC CCCGCAGAAC CACAGCATTT CCCTCTTTTT CTATTGGTTT 
CAGCTGGAAG ATTCAAGACA ATGCATGAAT GATTTCTTGG AGGTAAGAAA CGGCGGCAGC 
AGCACCTCAC CACTGCTTGA CAAGTACTGT AGCAACCTGC TGCCCAACCC GGTCTTCTCT 
CAGAGCAACG AACTGTATCT GCACTTTCAC AGCGACCACT CAGTCACCAA CAATGGCTAT 
GAAATTATTT GGACCTCCTC TGCTGCTGGC TGTGGAGGGA CTCTTTTGGG CGACGAAGGG 
ATATTCACCA ACCCTGGCTT TCCTGACAGT TACCCGAACA ACACTCATTG TGAATGGACC 
ATTGTTGCTC CTTCTGGAAG GCCTGTCTCT GTCGGGTTTC CCTTTCTCAG TATCGACTCT 
TCTGGTGGCT GTGACCAGAA CTACCTCATA GTCTTTAATG GTCCAGACGC CAACTCCCCA 
CCCTTTGGAC CGTTGTGTGG CATCAACACT GGGATAGCGC CCTTCTATGC TTCATCAAAT 
CGGGTCTTTA TAAGGTTTCA TGCTGAGTAT ACGACACGTC TTTCAGGGTT TGAAATAATG 
TGGAGCAGCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Diet induced obesity in rats reduces NHE3 and Na(+) /K(+) -ATPase expression in the kidney.
Clinical and experimental pharmacology & physiology42(10)1118-26(2015 Oct)
Briffa JF,Grinfeld E,Jenkin KA,Mathai ML,Poronnik P,McAinch AJ,Hryciw DH

Megalin/Cubulin-Lysosome-mediated Albumin Reabsorption Is Involved in the Tubular Cell Activation of NLRP3 Inflammasome and Tubulointerstitial Inflammation.
The Journal of biological chemistry290(29)18018-28(2015 Jul)
Liu D,Wen Y,Tang TT,Lv LL,Tang RN,Liu H,Ma KL,Crowley SD,Liu BC

Dab2, megalin, cubilin and amnionless receptor complex might mediate intestinal endocytosis in the suckling rat.
Journal of cellular biochemistry115(3)510-22(2014 Mar)
V?zquez-Carretero MD,Palomo M,Garc?a-Miranda P,S?nchez-Aguayo I,Peral MJ,Calonge ML,Ilundain AA

Impact of lactobacilli on orally acquired listeriosis.
Proceedings of the National Academy of Sciences of the United States of America109(41)16684-9(2012 Oct)
Archambaud C,Nahori MA,Soubigou G,B?cavin C,Laval L,Lechat P,Smokvina T,Langella P,Lecuit M,Cossart P

Cubilin is expressed in rat and human glomerular podocytes.
Nephrology, dialysis, transplantation : official publication of the European Dialysis and Transplant Association - European Renal Association27(8)3156-9(2012 Aug)
Prabakaran T,Christensen EI,Nielsen R,Verroust PJ

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Cubn cDNA ORF clone, Rattus norvegicus(Norway rat)

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

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