Atf4 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Atf4
Entrez Gene ID	79255
Full Name	activating transcription factor 4
General protein information	Preferred Names activating transcription factor 4 Names cyclic AMP-dependent transcription factor ATF-4 activating transcription factor 4 (tax-responsive enhancer element B67) activating transcription factor ATF-4 cAMP-dependent transcription factor ATF-4 rATF-4
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 7 Map Location: 7q34
Summary	binds GABA(B) receptors; may play a role in regulation of activity dependent gene expression [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
NM_024403.2	NP_077379.1	cyclic AMP-dependent transcription factor ATF-4

Pathways from BioSystems

Homologies

Homo sapiens (human)	ATF4	NP_001666.2
Canis lupus familiaris (dog)	ATF4	XP_859677.1
Mus musculus (house mouse)	Atf4	NP_001274109.1
Danio rerio (zebrafish)	atf4b2	NP_001096662.1
Xenopus tropicalis (tropical clawed frog)	atf4	NP_001004785.1
Danio rerio (zebrafish)	atf4b1	NP_998398.1
Pan troglodytes (chimpanzee)	ATF4	NP_001239446.1
	ATF4	XP_002798402.1
Bos taurus (cattle)	ATF4	NP_001029514.1
Rattus norvegicus (Norway rat)	Atf4	NP_077379.1
Gallus gallus (chicken)	ATF4	NP_990211.1

Gene Ontology
Bibliography

Related articles in PubMed

ATF4-Induced Metabolic Reprograming Is a Synthetic Vulnerability of the p62-Deficient Tumor Stroma.
Linares JF, Cordes T, Duran A, Reina-Campos M, Valencia T, Ahn CS, Castilla EA, Moscat J, Metallo CM, Diaz-Meco MT
Cell metabolism26(6)817-829.e6(2017 Dec)

Glucagon and Insulin Cooperatively Stimulate Fibroblast Growth Factor 21 Gene Transcription by Increasing the Expression of Activating Transcription Factor 4.
Alonge KM, Meares GP, Hillgartner FB
The Journal of biological chemistry292(13)5239-5252(2017 03)

Activating transcription factor-4 promotes mineralization in vascular smooth muscle cells.
Masuda M, Miyazaki-Anzai S, Keenan AL, Shiozaki Y, Okamura K, Chick WS, Williams K, Zhao X, Rahman SM, Tintut Y, Adams CM, Miyazaki M
JCI insight1(18)e88646(2016 Nov)

Activating Transcription Factor 4 (ATF4) modulates Rho GTPase levels and function via regulation of RhoGDI?.
Pasini S, Liu J, Corona C, Peze-Heidsieck E, Shelanski M, Greene LA
Scientific reports636952(2016 Nov)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

glucagon plus insulin increases FGF21 transcription by stimulating ATF4 expression
Title: Glucagon and Insulin Cooperatively Stimulate Fibroblast Growth Factor 21 Gene Transcription by Increasing the Expression of Activating Transcription Factor 4.

Study showed that activating transcription factor 4 (ATF4) does not protect nigral DA neurons against an human alpha-synuclein -induced pathology. The recombinant adeno-associate virus -mediated overexpression of ATF4 resulted in severe nigra-striatal degeneration via activation of caspases 3/7.
Title: Up-regulation of activating transcription factor 4 induces severe loss of dopamine nigral neurons in a rat model of Parkinson's disease.

Zn(2+) stimulated the phosphorylation of eIF2a and promoted the nuclear accumulation of activating transcription factor 4 (ATF4). Treatment with salubrinal enhanced Zn(2+)-induced ATF4 accumulation and IL-23 p19 mRNA expression.
Title: Zinc regulates expression of IL-23 p19 mRNA via activation of eIF2?/ATF4 axis in HAPI cells.

Data suggest that down-regulation of PERK kinase-activating transcription factor 4 (ATF4)-tramscription factor CHOP pathway by Astragaloside IV (AS-IV) may be a strategy for the treatment of diabetic nephropathy (DN).
Title: Down-regulation of PERK-ATF4-CHOP pathway by Astragaloside IV is associated with the inhibition of endoplasmic reticulum stress-induced podocyte apoptosis in diabetic rats.

ATF-4 is involved in endoplasmic reticulum stress-mediated apoptosis contributing to vascular calcification.
Title: Activating transcription factor 4 is involved in endoplasmic reticulum stress-mediated apoptosis contributing to vascular calcification.

The following Atf4 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Atf4 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa12659	NM_024403.2 Latest version!	Rattus norvegicus activating transcription factor 4 (Atf4), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$188.30 $269.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Atf4 ( NM_024403.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_077379.1 Rattus norvegicus activating transcription factor 4 (Atf4), mRNA.

Price & Availability↑

CloneID	ORa12659
Clone ID Related Accession (Same CDS sequence)	NM_024403.2
Accession Version	NM_024403.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1044bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-15
Organism	Rattus norvegicus(Norway rat)
Product	cyclic AMP-dependent transcription factor ATF-4
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AABR07058511.1 and BC158588.1. On or before Dec 15, 2013 this sequence version replaced XM_006242127.1, NM_024403.1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: FQ232762.1, FQ219043.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMD00052296, SAMD00052297 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## regulatory uORF :: PMID: 15277680 ##RefSeq-Attributes-END##

ATGACCGAGA TGAGCTTCCT GAACAGCGAA GTGTTGGCGG GGGACTTAAT GTCCCCCTTC 
GACCAGTCGG GTTTGGGGGC TGAAGAAAGC CTAGGTCTCT TAGATGACTA TCTGGAGGTG 
GCCAAGCACT TCAAACCTCA TGGGTTCTCC AGCGACAAGG CGGGCTCCTC AGAATGGCTG 
GCTATGGATG GGTTGGTCAG TGCCTCAGAC ACCGGCAAGG AGGATGCCTT TTCCGGGACA 
GATTGGATGT TGGAGAAAAT GGACCTGAAA GAGTTTGACT TCGATGCTCT GTTTCGAATG 
GATGACCTGG AAACCATGCC AGATGAGCTT TTGGCCACGT TGGATGACAC ATGTGATCTT 
TTTGCCCCTC TAGTCCAAGA GACTAATAAG GAGCCCCCTC AGACAGTGAA CCCAATTGGC 
CATCTCCCAG AAAGTGTAAT AAAAGTCGAC CAGGCTGCCC CCTTTACATT CTTGCAGCCT 
CTTCCCTGTT CCCCAGGGTT TCTGTCTTCC ACTCCAGATC ATTCCTTTAG TTTAGAGCTG 
GGAAGTGAGG TTGATATCTC TGAAGGAGAT AGGAAGCCTG ACTCTGCTGC TTATATTACT 
CTAACCCCTC AGTGTGTAAA GGAGGAAGAC ACTCCCTCTG ATAGTGACAG TGGCATCTGT 
ATGAGCCCTG AGTCCTACCT GGGCTCTCCC CAACACAGCC CTTCCACCTC CAGGGCCCCA 
CCAGACAGTC TGCCTTCTCC AGGTGTTCCT CGTGGTTCTC GACCCAAACC TTATGACCCA 
CCTGGAGTTA GTGTGACAGC TAAAGTGAAG ACTGAAAAGT TGGATAAGAA GCTGAAAAAG 
ATGGAGCAAA ACAAGACAGC AGCTACTAGG TACCGCCAGA AGAAGAGGGC TGAGCAGGAA 
GCCCTCACTG GCGAGTGTAA AGAGCTAGAA AAGAAGAACG AGGCTCTGAA AGAGAAGGCA 
GATTCTCTCG CCAAAGAGAT TCAGTATCTA AAAGACCTGA TAGAAGAGGT CCGTAAGGCA 
AGGGGGAAGA AGAGAGTTCC TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_077379.1
CDS	277..1320
Translation	MTEMSFLNSEVLAGDLMSPFDQSGLGAEESLGLLDDYLEVAKHFKPHGFSSDKAGSSEWLAMDGLVSASDTGKEDAFSGTDWMLEKMDLKEFDFDALFRMDDLETMPDELLATLDDTCDLFAPLVQETNKEPPQTVNPIGHLPESVIKVDQAAPFTFLQPLPCSPGFLSSTPDHSFSLELGSEVDISEGDRKPDSAAYITLTPQCVKEEDTPSDSDSGICMSPESYLGSPQHSPSTSRAPPDSLPSPGVPRGSRPKPYDPPGVSVTAKVKTEKLDKKLKKMEQNKTAATRYRQKKRAEQEALTGECKELEKKNEALKEKADSLAKEIQYLKDLIEEVRKARGKKRVP

Target ORF information:

RefSeq Version	NM_024403.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus activating transcription factor 4 (Atf4), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_024403.2

ORF Insert Sequence:

ATGACCGAGA TGAGCTTCCT GAACAGCGAA GTGTTGGCGG GGGACTTAAT GTCCCCCTTC 
GACCAGTCGG GTTTGGGGGC TGAAGAAAGC CTAGGTCTCT TAGATGACTA TCTGGAGGTG 
GCCAAGCACT TCAAACCTCA TGGGTTCTCC AGCGACAAGG CGGGCTCCTC AGAATGGCTG 
GCTATGGATG GGTTGGTCAG TGCCTCAGAC ACCGGCAAGG AGGATGCCTT TTCCGGGACA 
GATTGGATGT TGGAGAAAAT GGACCTGAAA GAGTTTGACT TCGATGCTCT GTTTCGAATG 
GATGACCTGG AAACCATGCC AGATGAGCTT TTGGCCACGT TGGATGACAC ATGTGATCTT 
TTTGCCCCTC TAGTCCAAGA GACTAATAAG GAGCCCCCTC AGACAGTGAA CCCAATTGGC 
CATCTCCCAG AAAGTGTAAT AAAAGTCGAC CAGGCTGCCC CCTTTACATT CTTGCAGCCT 
CTTCCCTGTT CCCCAGGGTT TCTGTCTTCC ACTCCAGATC ATTCCTTTAG TTTAGAGCTG 
GGAAGTGAGG TTGATATCTC TGAAGGAGAT AGGAAGCCTG ACTCTGCTGC TTATATTACT 
CTAACCCCTC AGTGTGTAAA GGAGGAAGAC ACTCCCTCTG ATAGTGACAG TGGCATCTGT 
ATGAGCCCTG AGTCCTACCT GGGCTCTCCC CAACACAGCC CTTCCACCTC CAGGGCCCCA 
CCAGACAGTC TGCCTTCTCC AGGTGTTCCT CGTGGTTCTC GACCCAAACC TTATGACCCA 
CCTGGAGTTA GTGTGACAGC TAAAGTGAAG ACTGAAAAGT TGGATAAGAA GCTGAAAAAG 
ATGGAGCAAA ACAAGACAGC AGCTACTAGG TACCGCCAGA AGAAGAGGGC TGAGCAGGAA 
GCCCTCACTG GCGAGTGTAA AGAGCTAGAA AAGAAGAACG AGGCTCTGAA AGAGAAGGCA 
GATTCTCTCG CCAAAGAGAT TCAGTATCTA AAAGACCTGA TAGAAGAGGT CCGTAAGGCA 
AGGGGGAAGA AGAGAGTTCC TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

ATF4-Induced Metabolic Reprograming Is a Synthetic Vulnerability of the p62-Deficient Tumor Stroma.
Cell metabolism26(6)817-829.e6(2017 Dec)
Linares JF,Cordes T,Duran A,Reina-Campos M,Valencia T,Ahn CS,Castilla EA,Moscat J,Metallo CM,Diaz-Meco MT

Glucagon and Insulin Cooperatively Stimulate Fibroblast Growth Factor 21 Gene Transcription by Increasing the Expression of Activating Transcription Factor 4.
The Journal of biological chemistry292(13)5239-5252(2017 03)
Alonge KM,Meares GP,Hillgartner FB

Activating transcription factor-4 promotes mineralization in vascular smooth muscle cells.
JCI insight1(18)e88646(2016 Nov)
Masuda M,Miyazaki-Anzai S,Keenan AL,Shiozaki Y,Okamura K,Chick WS,Williams K,Zhao X,Rahman SM,Tintut Y,Adams CM,Miyazaki M

Activating Transcription Factor 4 (ATF4) modulates Rho GTPase levels and function via regulation of RhoGDI?.
Scientific reports636952(2016 Nov)
Pasini S,Liu J,Corona C,Peze-Heidsieck E,Shelanski M,Greene LA

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Atf4 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

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gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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