Gemin5 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Gemin5
Entrez Gene ID	691231
Full Name	gem (nuclear organelle) associated protein 5
General protein information	Preferred Names gem (nuclear organelle) associated protein 5 Names gem-associated protein 5 rCG34955-like
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 10 Map Location: 10q22

mRNA and Protein(s)

mRNA	Protein	Name
NM_001172089.1	NP_001165560.1	gem-associated protein 5
XM_006246400.2	XP_006246462.1	gem-associated protein 5 isoform X5
XM_006246401.3	XP_006246463.1	gem-associated protein 5 isoform X6
XM_006246398.2	XP_006246460.1	gem-associated protein 5 isoform X3
XM_006246399.2	XP_006246461.1	gem-associated protein 5 isoform X4
XM_006246396.2	XP_006246458.1	gem-associated protein 5 isoform X1
XM_006246397.2	XP_006246459.1	gem-associated protein 5 isoform X2

Pathways from BioSystems

Homologies

	GEMIN5	XP_001112869.2
Danio rerio (zebrafish)	gemin5	XP_001339880.4
Homo sapiens (human)	GEMIN5	NP_056280.2
Xenopus tropicalis (tropical clawed frog)	gemin5	XP_004912844.1
Bos taurus (cattle)	GEMIN5	XP_003586382.1
Canis lupus familiaris (dog)	GEMIN5	XP_005619319.1
Mus musculus (house mouse)	Gemin5	NP_001160141.1
Gallus gallus (chicken)	GEMIN5	XP_414574.3
Pan troglodytes (chimpanzee)	GEMIN5	XP_527093.2
Rattus norvegicus (Norway rat)	Gemin5	NP_001165560.1

Gene Ontology
Bibliography

Related articles in PubMed

Structural basis for snRNA recognition by the double-WD40 repeat domain of Gemin5.
Jin W, Wang Y, Liu CP, Yang N, Jin M, Cong Y, Wang M, Xu RM
Genes & development30(21)2391-2403(2016 Nov)

Structural insights into Gemin5-guided selection of pre-snRNAs for snRNP assembly.
Xu C, Ishikawa H, Izumikawa K, Li L, He H, Nobe Y, Yamauchi Y, Shahjee HM, Wu XH, Yu YT, Isobe T, Takahashi N, Min J
Genes & development30(21)2376-2390(2016 Nov)

The RNA-binding protein Gemin5 binds directly to the ribosome and regulates global translation.
Francisco-Velilla R, Fernandez-Chamorro J, Ramajo J, Martinez-Salas E
Nucleic acids research44(17)8335-51(2016 Sep)

Gemin5 Binds to the Survival Motor Neuron mRNA to Regulate SMN Expression.
Workman E, Kalda C, Patel A, Battle DJ
The Journal of biological chemistry290(25)15662-9(2015 Jun)

Insights into RNA biology from an atlas of mammalian mRNA-binding proteins.
Castello A, Fischer B, Eichelbaum K, Horos R, Beckmann BM, Strein C, Davey NE, Humphreys DT, Preiss T, Steinmetz LM, Krijgsveld J, Hentze MW
Cell149(6)1393-406(2012 Jun)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Gemin5 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Gemin5 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ORa33122	XM_006246401.3 Latest version!	Rattus norvegicus gem (nuclear organelle) associated protein 5 (Gemin5), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$643.30 $919.00
ORa06432	NM_001172089.1 Latest version!	Rattus norvegicus gem (nuclear organelle) associated protein 5 (Gemin5), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$727.30 $1039.00
ORa33117	XM_006246396.2 Latest version!	Rattus norvegicus gem (nuclear organelle) associated protein 5 (Gemin5), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$727.30 $1039.00
ORa33118	XM_006246397.2 Latest version!	Rattus norvegicus gem (nuclear organelle) associated protein 5 (Gemin5), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$727.30 $1039.00
ORa33119	XM_006246398.2 Latest version!	Rattus norvegicus gem (nuclear organelle) associated protein 5 (Gemin5), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$727.30 $1039.00
ORa33120	XM_006246399.2 Latest version!	Rattus norvegicus gem (nuclear organelle) associated protein 5 (Gemin5), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$727.30 $1039.00
ORa33121	XM_006246400.2 Latest version!	Rattus norvegicus gem (nuclear organelle) associated protein 5 (Gemin5), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$727.30 $1039.00

ORF Online Only Promotion

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Gemin5 ( XM_006246401.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006246463.1 Rattus norvegicus gem (nuclear organelle) associated protein 5 (Gemin5), transcript variant X6, mRNA.

Price & Availability↑

CloneID	ORa33122
Clone ID Related Accession (Same CDS sequence)	XM_006246401.3
Accession Version	XM_006246401.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3711bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	gem-associated protein 5 isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005109.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006246401.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTTTTGA AATTGCCCTT TCTGAAGAGA AGAAGTGGGG GTGTGGACCC AACAGTTAAA 
GAACGTCTTT GGTTGACACT TCATTGGCCC AAGAACCAAC CAACACAGCT GGTATCCAGT 
TGTTTTGGAG GTGAGCTGCT GCTGTGGGAC CTCACCCAGT CATGGAGACG GAAGTACACG 
CTCTTCAGCA CCTCAGCGGA AGGGCACAAT CACTCCAGAA TTGTCTTCAA CTTGTGTCCT 
TTGAAGACTG AGGATGGCAA ACAGCTGCTA TTGTCCACGT CCATGGACAG AGATGTAAAA 
TGTTGGGACA TGGCCACCCT GGAGTGTTGC TGGACATTAC CTTCTCTTGG AGGTTTTGCC 
TACAGCCTGG CCTTTTCACC TGTGGACGTG GGCTCCCTGG CCATAGGTGT TGGGGACGGC 
ATGATCCGGG TATGGAATAC ACTCTCCATA AAGAACAACT ACGATGTGAA AAACTTCTGG 
CAAGGAGTCA AGTCCAAGGT CACGGCGCTA TGCTGGCATC CAAACAAGGA GGGCTGCTTA 
GCCTTTGGGA CCGATGACGG AAAAGTGGGA TTGTATGACA CCTGCTCCAA CAAACCTCCA 
CAGATTTCAA GCACATATCA TAAGAAGACC GTATATAGGT TGGCTTGGGG GCCACCAGTG 
CCCCCCATGT CACTTGGAGG GGAAGGAGAC AGGCCTTCCC TCACCTTGTA CAGCTGTGGA 
GGGGAAGGCA TTGTGCTGCA GCATAATCCC TGGAAGCTCA GTGGAGAGGC CTTTGACATC 
AACAAACTCA TGAGAGACAC CAACTCCATT AGATACAAGC TGCCTGTGCA CACAGAGATC 
AGCTGGAAAG GAGATGGCAA AGTCATGGCT CTTGGAAATG AAGATGGATC AATAGAAATA 
TTCCAAGTCC CAAACTTGAA ACTGCTCTGC ACCATCCAGC AGCACCACAA GCTTGTGAAC 
GCCATAGTCT GGCACCATGA GCATGGCAGC CGTCCAGAGC TGAGCTGCCT CCTTGCTTCT 
GGCTCCAACA ATGCAGTTAT CTACGTGCAC AACCTGAAAG CTGTCCTAGA GAGCAATCCT 
GAGTCTCCAA TAACCATCAC AGAGCCCTAC CGGACCCTCT CAGGGCACAC AGCCAAGGTT 
ACCAGTCTGG CATGGAGCCC ACATCATGAT GGAAGGCTGG TATCTGCTTG CTACGATGGC 
ACAGCTCAGG TGTGGGATGC CCTCCGAGAA GAACCTTTGT TCAATTTCCG AGGACATCGA 
GGCCGGCTGT TATGTGTTGC GTGGTCCCCA GTGGATCCAG AATGCATCTA CTCAGGTGCA 
GATGACTTCT GTGTCTACAG GTGGCTGACC TCCATGCAGG ACCATTCCCG GCCTCCTCAA 
GGGAAAAAAT GTATTGAATT AGAGAAAAAA CGGCTCTCTC AATTTAAGCC AAAGCTAAAA 
AAGAAGAAAA AACCTACCTT GCGACTGCCC ATAAAGCAAG ATTCATCCAT TGGCAATGAA 
GATGAGAGTG TGAGGGAGAA CTTGGCACCT GTTGAGAATG GCTTGTCCGA TCAGGATGGT 
GAGGAGGAAG CCCAGGAGCC GGAGCTGCCT CCCTCTCCCG TGGTTTGTGG AGAACCTGTT 
TCCTGCACTG CTGTCTCCTT AGGCTTGGAA AAGTCAAAAG TCACTGTTAC TAGTAAAGTC 
GCCTCACTGA AAAAGGAGCC ACCTAAAGAG AAGCCAGAAG CCTTGCTCAA GAAGAGGAAG 
GCTCGCTCCA TGCTCCCCCT GAGCACCAGC TTAGACCACA GGTCCAAAGA GGAGCTCCAT 
CGAGACTGTT TGGTTCTAGC AACGGCAACA CACGCCAGAG CAGAACTGCA TGAAGATGTG 
TCTGCCGACC TTGAGGAACG ATTTCACTTG GGCCTTTTCA CAGACAGGGC TGCTCTGTAC 
AGGATGATGG AGACAGAGGG GAAAGGTCAC TTGGAAAGTG GCCACCCCGA GCTTTTTCAC 
CAGCTCATGC TCTGGAAGGG AGATCTGAAA GGCGTTCTCC AGGCTGCAGC AGAGAGAGGC 
GAGCTCACAG ACAGTCTCGT GGCCGTGGCA CCAGTAGCTG GCTACAGTGT GTGGCTGTGG 
GCTGTGGAAG CCTTTGCCAA GCAGCTGTGT TTCCAGGATC AGTATGTCAA GGCTGCCTCC 
TACCTGCTAT CCATCCACAG AGTGTACGAA GCTGTGGAGC TTCTCAGGTC AAACCATCTC 
TACAGAGAAG CTATTGCAGT TGCCAAGGCT CGGCTGCGCC CTGAGGACCC TGTTCTGAAG 
GACCTGTACC TCAGCTGGGG GTCCATCCTG GAGAGAGATG GCCACTATGC AATTGCAGCC 
AAGTGCTACT TAGGAGCCAC TTCCGCCTAT GATGCAGCCA AAGTCCTAGC CAGAAAGGGA 
GATGCAGCCT CTCTTAGAAC AGCTGCAGAG CTGGCTGCCA TAGCAGGAGA GAGTGAGCTG 
GCTGCTTCCC TGGCTCTTAG GTGCGCCCAA GAGCTGCTTC TGCTGAAGAA CTGGGTGGGT 
GCACAGGAAG CCTTGGGACT GCATGAAAGC CTGCAGGGCC AGAGACTGGT GTTCTGCCTC 
CTTGAGCTTC TATGCCGGCA CCTGGAGGAA AAACAGCCTC CAGAGGTCAG AGGCCCCTCT 
TCCATTTACC ACCAGTGGGC CACTGGTTCT GAGGGAACCT TGGTGCAGAG AGTAACTGGT 
GTGTGGCGCA GCGCCTTCAG TGTGGACACC CCGGAGCAGT GTCGGGCCGC CTTGCAGAAG 
CTACAGGATG TCAAGTACCC ATCAGCAACA AGTAACACTC CCTTCAGACA GCTGCTGCTT 
CATGTCTGCC ATGACCTAAC CTTAGCAATG CTGAGCCAGC AGGCTGCTTC CTGGGAGGAG 
GCTGTCCCTG CACTGCTGCA GGCTGTGGTT CGGAGCTACA CCTCAGGGAA TTTCACCCTC 
ATGCAAGAAA TCTACTCAGC CTTTCTCCCA GGCGGCTGTG ACCACCTACG AGACAAACTA 
GGTGACCTAT CTCCTACCAT GGCGGCTTTC AAAAGCTTAG AGGCCTTTTT CATTTATGGG 
CAACTGTATG AAGTCTGGTG GTCACTCTGT GGGCCTGGCT CAGAATCAAG CATCTGGGTG 
TGGTCGGCTG AAAGCACAGT CTCTGATAAA CAGAGCAAGC CAGGGGACAG TGCCAGTGCT 
GAAGACATGG AGCAGCCTCT AGGCCCAGGC CTGAGGCTCA GTGCGGAGAG TGAACGTTTG 
CTGAGTGCCT GCAAGGAGCT CTTCTCAGAG AGGCATGCCA GCCTACAGAC CTCCCAGAGG 
ACTGTTGCTG AAGTCCAAGA GACGCTGGCA GAGATGATCC GCCAGCACCA AAAGAGTCAG 
CTCTGCAAGG CCACAGCCAA CGGCCCCAAC AGAGATGAAC CCAGCCAGCA AGCAGAGCGG 
GCCCCTTCCC AACCTCCGAG CCCAACCAGA GAAGAAAGAA ATGCACCGGT GTCTCTCCCT 
GAATTAACCA GAAGGCTCAC AGAGGCAAAC GAGAGAATAG CTGAGTTTCC AGAGAGTGTA 
AAGGCCTGGC CCTTTCCAGA TGTGCTGGAG TGCTGTCTCG TCTTACTTCA CATCGGGTCC 
CAGTGTCCTG GTGCTGTGAA CCCTGAGATG CAGCAACAGG CCCAGAAGCT CCTTCAGAAG 
TATGGCCACA CTAGAGCTTA CAGAAGACAC TGCCAGATTC GACACACATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006246463.1
CDS	860..4570
Translation	MVLKLPFLKRRSGGVDPTVKERLWLTLHWPKNQPTQLVSSCFGGELLLWDLTQSWRRKYTLFSTSAEGHNHSRIVFNLCPLKTEDGKQLLLSTSMDRDVKCWDMATLECCWTLPSLGGFAYSLAFSPVDVGSLAIGVGDGMIRVWNTLSIKNNYDVKNFWQGVKSKVTALCWHPNKEGCLAFGTDDGKVGLYDTCSNKPPQISSTYHKKTVYRLAWGPPVPPMSLGGEGDRPSLTLYSCGGEGIVLQHNPWKLSGEAFDINKLMRDTNSIRYKLPVHTEISWKGDGKVMALGNEDGSIEIFQVPNLKLLCTIQQHHKLVNAIVWHHEHGSRPELSCLLASGSNNAVIYVHNLKAVLESNPESPITITEPYRTLSGHTAKVTSLAWSPHHDGRLVSACYDGTAQVWDALREEPLFNFRGHRGRLLCVAWSPVDPECIYSGADDFCVYRWLTSMQDHSRPPQGKKCIELEKKRLSQFKPKLKKKKKPTLRLPIKQDSSIGNEDESVRENLAPVENGLSDQDGEEEAQEPELPPSPVVCGEPVSCTAVSLGLEKSKVTVTSKVASLKKEPPKEKPEALLKKRKARSMLPLSTSLDHRSKEELHRDCLVLATATHARAELHEDVSADLEERFHLGLFTDRAALYRMMETEGKGHLESGHPELFHQLMLWKGDLKGVLQAAAERGELTDSLVAVAPVAGYSVWLWAVEAFAKQLCFQDQYVKAASYLLSIHRVYEAVELLRSNHLYREAIAVAKARLRPEDPVLKDLYLSWGSILERDGHYAIAAKCYLGATSAYDAAKVLARKGDAASLRTAAELAAIAGESELAASLALRCAQELLLLKNWVGAQEALGLHESLQGQRLVFCLLELLCRHLEEKQPPEVRGPSSIYHQWATGSEGTLVQRVTGVWRSAFSVDTPEQCRAALQKLQDVKYPSATSNTPFRQLLLHVCHDLTLAMLSQQAASWEEAVPALLQAVVRSYTSGNFTLMQEIYSAFLPGGCDHLRDKLGDLSPTMAAFKSLEAFFIYGQLYEVWWSLCGPGSESSIWVWSAESTVSDKQSKPGDSASAEDMEQPLGPGLRLSAESERLLSACKELFSERHASLQTSQRTVAEVQETLAEMIRQHQKSQLCKATANGPNRDEPSQQAERAPSQPPSPTREERNAPVSLPELTRRLTEANERIAEFPESVKAWPFPDVLECCLVLLHIGSQCPGAVNPEMQQQAQKLLQKYGHTRAYRRHCQIRHT

Target ORF information:

RefSeq Version	XM_006246401.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus gem (nuclear organelle) associated protein 5 (Gemin5), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006246401.3

ORF Insert Sequence:

ATGGTTTTGA AATTGCCCTT TCTGAAGAGA AGAAGTGGGG GTGTGGACCC AACAGTTAAA 
GAACGTCTTT GGTTGACACT TCATTGGCCC AAGAACCAAC CAACACAGCT GGTATCCAGT 
TGTTTTGGAG GTGAGCTGCT GCTGTGGGAC CTCACCCAGT CATGGAGACG GAAGTACACG 
CTCTTCAGCA CCTCAGCGGA AGGGCACAAT CACTCCAGAA TTGTCTTCAA CTTGTGTCCT 
TTGAAGACTG AGGATGGCAA ACAGCTGCTA TTGTCCACGT CCATGGACAG AGATGTAAAA 
TGTTGGGACA TGGCCACCCT GGAGTGTTGC TGGACATTAC CTTCTCTTGG AGGTTTTGCC 
TACAGCCTGG CCTTTTCACC TGTGGACGTG GGCTCCCTGG CCATAGGTGT TGGGGACGGC 
ATGATCCGGG TATGGAATAC ACTCTCCATA AAGAACAACT ACGATGTGAA AAACTTCTGG 
CAAGGAGTCA AGTCCAAGGT CACGGCGCTA TGCTGGCATC CAAACAAGGA GGGCTGCTTA 
GCCTTTGGGA CCGATGACGG AAAAGTGGGA TTGTATGACA CCTGCTCCAA CAAACCTCCA 
CAGATTTCAA GCACATATCA TAAGAAGACC GTATATAGGT TGGCTTGGGG GCCACCAGTG 
CCCCCCATGT CACTTGGAGG GGAAGGAGAC AGGCCTTCCC TCACCTTGTA CAGCTGTGGA 
GGGGAAGGCA TTGTGCTGCA GCATAATCCC TGGAAGCTCA GTGGAGAGGC CTTTGACATC 
AACAAACTCA TGAGAGACAC CAACTCCATT AGATACAAGC TGCCTGTGCA CACAGAGATC 
AGCTGGAAAG GAGATGGCAA AGTCATGGCT CTTGGAAATG AAGATGGATC AATAGAAATA 
TTCCAAGTCC CAAACTTGAA ACTGCTCTGC ACCATCCAGC AGCACCACAA GCTTGTGAAC 
GCCATAGTCT GGCACCATGA GCATGGCAGC CGTCCAGAGC TGAGCTGCCT CCTTGCTTCT 
GGCTCCAACA ATGCAGTTAT CTACGTGCAC AACCTGAAAG CTGTCCTAGA GAGCAATCCT 
GAGTCTCCAA TAACCATCAC AGAGCCCTAC CGGACCCTCT CAGGGCACAC AGCCAAGGTT 
ACCAGTCTGG CATGGAGCCC ACATCATGAT GGAAGGCTGG TATCTGCTTG CTACGATGGC 
ACAGCTCAGG TGTGGGATGC CCTCCGAGAA GAACCTTTGT TCAATTTCCG AGGACATCGA 
GGCCGGCTGT TATGTGTTGC GTGGTCCCCA GTGGATCCAG AATGCATCTA CTCAGGTGCA 
GATGACTTCT GTGTCTACAG GTGGCTGACC TCCATGCAGG ACCATTCCCG GCCTCCTCAA 
GGGAAAAAAT GTATTGAATT AGAGAAAAAA CGGCTCTCTC AATTTAAGCC AAAGCTAAAA 
AAGAAGAAAA AACCTACCTT GCGACTGCCC ATAAAGCAAG ATTCATCCAT TGGCAATGAA 
GATGAGAGTG TGAGGGAGAA CTTGGCACCT GTTGAGAATG GCTTGTCCGA TCAGGATGGT 
GAGGAGGAAG CCCAGGAGCC GGAGCTGCCT CCCTCTCCCG TGGTTTGTGG AGAACCTGTT 
TCCTGCACTG CTGTCTCCTT AGGCTTGGAA AAGTCAAAAG TCACTGTTAC TAGTAAAGTC 
GCCTCACTGA AAAAGGAGCC ACCTAAAGAG AAGCCAGAAG CCTTGCTCAA GAAGAGGAAG 
GCTCGCTCCA TGCTCCCCCT GAGCACCAGC TTAGACCACA GGTCCAAAGA GGAGCTCCAT 
CGAGACTGTT TGGTTCTAGC AACGGCAACA CACGCCAGAG CAGAACTGCA TGAAGATGTG 
TCTGCCGACC TTGAGGAACG ATTTCACTTG GGCCTTTTCA CAGACAGGGC TGCTCTGTAC 
AGGATGATGG AGACAGAGGG GAAAGGTCAC TTGGAAAGTG GCCACCCCGA GCTTTTTCAC 
CAGCTCATGC TCTGGAAGGG AGATCTGAAA GGCGTTCTCC AGGCTGCAGC AGAGAGAGGC 
GAGCTCACAG ACAGTCTCGT GGCCGTGGCA CCAGTAGCTG GCTACAGTGT GTGGCTGTGG 
GCTGTGGAAG CCTTTGCCAA GCAGCTGTGT TTCCAGGATC AGTATGTCAA GGCTGCCTCC 
TACCTGCTAT CCATCCACAG AGTGTACGAA GCTGTGGAGC TTCTCAGGTC AAACCATCTC 
TACAGAGAAG CTATTGCAGT TGCCAAGGCT CGGCTGCGCC CTGAGGACCC TGTTCTGAAG 
GACCTGTACC TCAGCTGGGG GTCCATCCTG GAGAGAGATG GCCACTATGC AATTGCAGCC 
AAGTGCTACT TAGGAGCCAC TTCCGCCTAT GATGCAGCCA AAGTCCTAGC CAGAAAGGGA 
GATGCAGCCT CTCTTAGAAC AGCTGCAGAG CTGGCTGCCA TAGCAGGAGA GAGTGAGCTG 
GCTGCTTCCC TGGCTCTTAG GTGCGCCCAA GAGCTGCTTC TGCTGAAGAA CTGGGTGGGT 
GCACAGGAAG CCTTGGGACT GCATGAAAGC CTGCAGGGCC AGAGACTGGT GTTCTGCCTC 
CTTGAGCTTC TATGCCGGCA CCTGGAGGAA AAACAGCCTC CAGAGGTCAG AGGCCCCTCT 
TCCATTTACC ACCAGTGGGC CACTGGTTCT GAGGGAACCT TGGTGCAGAG AGTAACTGGT 
GTGTGGCGCA GCGCCTTCAG TGTGGACACC CCGGAGCAGT GTCGGGCCGC CTTGCAGAAG 
CTACAGGATG TCAAGTACCC ATCAGCAACA AGTAACACTC CCTTCAGACA GCTGCTGCTT 
CATGTCTGCC ATGACCTAAC CTTAGCAATG CTGAGCCAGC AGGCTGCTTC CTGGGAGGAG 
GCTGTCCCTG CACTGCTGCA GGCTGTGGTT CGGAGCTACA CCTCAGGGAA TTTCACCCTC 
ATGCAAGAAA TCTACTCAGC CTTTCTCCCA GGCGGCTGTG ACCACCTACG AGACAAACTA 
GGTGACCTAT CTCCTACCAT GGCGGCTTTC AAAAGCTTAG AGGCCTTTTT CATTTATGGG 
CAACTGTATG AAGTCTGGTG GTCACTCTGT GGGCCTGGCT CAGAATCAAG CATCTGGGTG 
TGGTCGGCTG AAAGCACAGT CTCTGATAAA CAGAGCAAGC CAGGGGACAG TGCCAGTGCT 
GAAGACATGG AGCAGCCTCT AGGCCCAGGC CTGAGGCTCA GTGCGGAGAG TGAACGTTTG 
CTGAGTGCCT GCAAGGAGCT CTTCTCAGAG AGGCATGCCA GCCTACAGAC CTCCCAGAGG 
ACTGTTGCTG AAGTCCAAGA GACGCTGGCA GAGATGATCC GCCAGCACCA AAAGAGTCAG 
CTCTGCAAGG CCACAGCCAA CGGCCCCAAC AGAGATGAAC CCAGCCAGCA AGCAGAGCGG 
GCCCCTTCCC AACCTCCGAG CCCAACCAGA GAAGAAAGAA ATGCACCGGT GTCTCTCCCT 
GAATTAACCA GAAGGCTCAC AGAGGCAAAC GAGAGAATAG CTGAGTTTCC AGAGAGTGTA 
AAGGCCTGGC CCTTTCCAGA TGTGCTGGAG TGCTGTCTCG TCTTACTTCA CATCGGGTCC 
CAGTGTCCTG GTGCTGTGAA CCCTGAGATG CAGCAACAGG CCCAGAAGCT CCTTCAGAAG 
TATGGCCACA CTAGAGCTTA CAGAAGACAC TGCCAGATTC GACACACATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gemin5 ( NM_001172089.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_001165560.1 Rattus norvegicus gem (nuclear organelle) associated protein 5 (Gemin5), mRNA.

Price & Availability↑

CloneID	ORa06432
Clone ID Related Accession (Same CDS sequence)	NM_001172089.1
Accession Version	NM_001172089.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4494bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-27
Organism	Rattus norvegicus(Norway rat)
Product	gem-associated protein 5
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AABR07029664.1. On Feb 2, 2010 this sequence version replaced XM_001074875.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMD00052296, SAMD00052297 [ECO:0000350] ##Evidence-Data-END##

ATGAAACCAG AGCCGCGGAC GCTTCCGCCG TCCCCCAACT GGTACTGCGC GCGCTGCAGC 
GACGCGGCGC CCGGAGGCAT CTTTGGCTTC GCTGCGCGGA CCTCCGTCTT CCTCGTCCGC 
GTGGGCCCGG GCGCCGGCGC GAGCCCAGGG GCGCCCCCGT TTCGAGTTGT AGGAGAGTTG 
GTGGGACACA CTGAAAGAGT ATCTGGTTTC ACTTTTTCTC ATCACCCTGG ACAATACAAC 
CTCTGTGCCA CCAGCTCAGA TGATGGGACT GTGAAGATTT GGGATGTAGA GACCAAGGCT 
GTAGTGACAG AACACGCGCT CCATCAGCAT ACAATATCAG CACTGCATTG GTCTCCGACA 
GTTAAGGACT TAATAGTCTC TGGGGATGAA AAAGGAGTTG TTTTCTGTTA CTGGCTTAAC 
AGAAGTGACA GCCAGCACCT CTTCACAGAG CCCAGGGCCA TATTCTGTCT GACCTGTTCA 
CCTCATCACG AAAATCTAGT AGCCATTGGC TACAAAGACG GCATAGTGAT TATCATTGAC 
ATCAGTAAAA AGGGAGAAGT TGTTCACAGG CTTCGAGGGC ACGACGATGA AATCCACTCC 
ATAGCCTGGT GTCCTTTGTC TGGTGAAGAC TGCTTATCTA TAAACCAAGA GGAAAACTCA 
GAAGAACCTG ACATTCCCAA TGGGAAGCTT ATAGCAGAAA CCCCCATCAC AAAAGGCTGC 
TACTTAGCTA CAGGAAGCAA GGATCAGACC ATTCGAATCT GGAGCTGTTC CCGAGGCCGG 
GGTGTAATGG TTTTGAAATT GCCCTTTCTG AAGAGAAGAA GTGGGGGTGT GGACCCAACA 
GTTAAAGAAC GTCTTTGGTT GACACTTCAT TGGCCCAAGA ACCAACCAAC ACAGCTGGTA 
TCCAGTTGTT TTGGAGGTGA GCTGCTGCTG TGGGACCTCA CCCAGTCATG GAGACGGAAG 
TACACGCTCT TCAGCACCTC AGCGGAAGGG CACAATCACT CCAGAATTGT CTTCAACTTG 
TGTCCTTTGA AGACTGAGGA TGGCAAACAG CTGCTATTGT CCACGTCCAT GGACAGAGAT 
GTAAAATGTT GGGACATGGC CACCCTGGAG TGTTGCTGGA CATTACCTTC TCTTGGAGGT 
TTTGCCTACA GCCTGGCCTT TTCACCTGTG GACGTGGGCT CCCTGGCCAT AGGTGTTGGG 
GACGGCATGA TCCGGGTATG GAATACACTC TCCATAAAGA ACAACTACGA TGTGAAAAAC 
TTCTGGCAAG GAGTCAAGTC CAAGGTCACG GCGCTATGCT GGCATCCAAA CAAGGAGGGC 
TGCTTAGCCT TTGGGACCGA TGACGGAAAA GTGGGATTGT ATGACACCTG CTCCAACAAA 
CCTCCACAGA TTTCAAGCAC ATATCATAAG AAGACCGTAT ATAGGTTGGC TTGGGGGCCA 
CCAGTGCCCC CCATGTCACT TGGAGGGGAA GGAGACAGGC CTTCCCTCAC CTTGTACAGC 
TGTGGAGGGG AAGGCATTGT GCTGCAGCAT AATCCCTGGA AGCTCAGTGG AGAGGCCTTT 
GACATCAACA AACTCATGAG AGACACCAAC TCCATTAGAT ACAAGCTGCC TGTGCACACA 
GAGATCAGCT GGAAAGGAGA TGGCAAAGTC ATGGCTCTTG GAAATGAAGA TGGATCAATA 
GAAATATTCC AAGTCCCAAA CTTGAAACTG CTCTGCACCA TCCAGCAGCA CCACAAGCTT 
GTGAACGCCA TAGTCTGGCA CCATGAGCAT GGCAGCCGTC CAGAGCTGAG CTGCCTCCTT 
GCTTCTGGCT CCAACAATGC AGTTATCTAC GTGCACAACC TGAAAGCTGT CCTAGAGAGC 
AATCCTGAGT CTCCAATAAC CATCACAGAG CCCTACCGGA CCCTCTCAGG GCACACAGCC 
AAGGTTACCA GTCTGGCATG GAGCCCACAT CATGATGGAA GGCTGGTATC TGCTTGCTAC 
GATGGCACAG CTCAGGTGTG GGATGCCCTC CGAGAAGAAC CTTTGTTCAA TTTCCGAGGA 
CATCGAGGCC GGCTGTTATG TGTTGCGTGG TCCCCAGTGG ATCCAGAATG CATCTACTCA 
GGTGCAGATG ACTTCTGTGT CTACAGGTGG CTGACCTCCA TGCAGGACCA TTCCCGGCCT 
CCTCAAGGGA AAAAATGTAT TGAATTAGAG AAAAAACGGC TCTCTCAATT TAAGCCAAAG 
CTAAAAAAGA AGAAAAAACC TACCTTGCGA CTGCCCATAA AGCAAGATTC ATCCATTGGC 
AATGAAGATG AGAGTGTGAG GGAGAACTTG GCACCTGTTG AGAATGGCTT GTCCGATCAG 
GATGGTGAGG AGGAAGCCCA GGAGCCGGAG CTGCCTCCCT CTCCCGTGGT TTGTGGAGAA 
CCTGTTTCCT GCACTGCTGT CTCCTTAGGC TTGGAAAAGT CAAAAGTCAC TGTTACTAGT 
AAAGTCGCCT CACTGAAAAA GGAGCCACCT AAAGAGAAGC CAGAAGCCTT GCTCAAGAAG 
AGGAAGGCTC GCTCCATGCT CCCCCTGAGC ACCAGCTTAG ACCACAGGTC CAAAGAGGAG 
CTCCATCGAG ACTGTTTGGT TCTAGCAACG GCAACACACG CCAGAGCAGA ACTGCATGAA 
GATGTGTCTG CCGACCTTGA GGAACGATTT CACTTGGGCC TTTTCACAGA CAGGGCTGCT 
CTGTACAGGA TGATGGAGAC AGAGGGGAAA GGTCACTTGG AAAGTGGCCA CCCCGAGCTT 
TTTCACCAGC TCATGCTCTG GAAGGGAGAT CTGAAAGGCG TTCTCCAGGC TGCAGCAGAG 
AGAGGCGAGC TCACAGACAG TCTCGTGGCC GTGGCACCAG TAGCTGGCTA CAGTGTGTGG 
CTGTGGGCTG TGGAAGCCTT TGCCAAGCAG CTGTGTTTCC AGGATCAGTA TGTCAAGGCT 
GCCTCCTACC TGCTATCCAT CCACAGAGTG TACGAAGCTG TGGAGCTTCT CAGGTCAAAC 
CATCTCTACA GAGAAGCTAT TGCAGTTGCC AAGGCTCGGC TGCGCCCTGA GGACCCTGTT 
CTGAAGGACC TGTACCTCAG CTGGGGGTCC ATCCTGGAGA GAGATGGCCA CTATGCAATT 
GCAGCCAAGT GCTACTTAGG AGCCACTTCC GCCTATGATG CAGCCAAAGT CCTAGCCAGA 
AAGGGAGATG CAGCCTCTCT TAGAACAGCT GCAGAGCTGG CTGCCATAGC AGGAGAGAGT 
GAGCTGGCTG CTTCCCTGGC TCTTAGGTGC GCCCAAGAGC TGCTTCTGCT GAAGAACTGG 
GTGGGTGCAC AGGAAGCCTT GGGACTGCAT GAAAGCCTGC AGGGCCAGAG ACTGGTGTTC 
TGCCTCCTTG AGCTTCTATG CCGGCACCTG GAGGAAAAAC AGCCTCCAGA GGTCAGAGGC 
CCCTCTTCCA TTTACCACCA GTGGGCCACT GGTTCTGAGG GAACCTTGGT GCAGAGAGTA 
ACTGGTGTGT GGCGCAGCGC CTTCAGTGTG GACACCCCGG AGCAGTGTCG GGCCGCCTTG 
CAGAAGCTAC AGGATGTCAA GTACCCATCA GCAACAAGTA ACACTCCCTT CAGACAGCTG 
CTGCTTCATG TCTGCCATGA CCTAACCTTA GCAATGCTGA GCCAGCAGGC TGCTTCCTGG 
GAGGAGGCTG TCCCTGCACT GCTGCAGGCT GTGGTTCGGA GCTACACCTC AGGGAATTTC 
ACCCTCATGC AAGAAATCTA CTCAGCCTTT CTCCCAGGCG GCTGTGACCA CCTACGAGAC 
AAACTAGGTG ACCTATCTCC TACCATGGCG GCTTTCAAAA GCTTAGAGGC CTTTTTCATT 
TATGGGCAAC TGTATGAAGT CTGGTGGTCA CTCTGTGGGC CTGGCTCAGA ATCAAGCATC 
TGGGTGTGGT CGGCTGAAAG CACAGTCTCT GATAAACAGA GCAAGCCAGG GGACAGTGCC 
AGTGCTGAAG ACATGGAGCA GCCTCTAGGC CCAGGCCTGA GGCTCAGTGC GGAGAGTGAA 
CGTTTGCTGA GTGCCTGCAA GGAGCTCTTC TCAGAGAGGC ATGCCAGCCT ACAGACCTCC 
CAGAGGACTG TTGCTGAAGT CCAAGAGACG CTGGCAGAGA TGATCCGCCA GCACCAAAAG 
AGTCAGCTCT GCAAGGCCAC AGCCAACGGC CCCAACAGAG ATGAACCCAG CCAGCAAGCA 
GAGCGGGCCC CTTCCCAACC TCCGAGCCCA ACAGAAGAAA GAAATGCACC GGTGTCTCTC 
CCTGAATTAA CCAGAAGGCT CACAGAGGCA AACGAGAGAA TAGCTGAGTT TCCAGAGAGT 
GTAAAGGCCT GGCCCTTTCC AGATGTGCTG GAGTGCTGTC TCGTCTTACT TCACATCGGG 
TCCCAGTGTC CTGGTGCTGT GAACCCTGAG ATGCAGCAAC AGGCCCAGAA GCTCCTTCAG 
AAGTATGGCC ACACTAGAGC TTACAGAAGA CACTGCCAGA TTCGACACAC ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001165560.1
CDS	84..4577
Translation	MKPEPRTLPPSPNWYCARCSDAAPGGIFGFAARTSVFLVRVGPGAGASPGAPPFRVVGELVGHTERVSGFTFSHHPGQYNLCATSSDDGTVKIWDVETKAVVTEHALHQHTISALHWSPTVKDLIVSGDEKGVVFCYWLNRSDSQHLFTEPRAIFCLTCSPHHENLVAIGYKDGIVIIIDISKKGEVVHRLRGHDDEIHSIAWCPLSGEDCLSINQEENSEEPDIPNGKLIAETPITKGCYLATGSKDQTIRIWSCSRGRGVMVLKLPFLKRRSGGVDPTVKERLWLTLHWPKNQPTQLVSSCFGGELLLWDLTQSWRRKYTLFSTSAEGHNHSRIVFNLCPLKTEDGKQLLLSTSMDRDVKCWDMATLECCWTLPSLGGFAYSLAFSPVDVGSLAIGVGDGMIRVWNTLSIKNNYDVKNFWQGVKSKVTALCWHPNKEGCLAFGTDDGKVGLYDTCSNKPPQISSTYHKKTVYRLAWGPPVPPMSLGGEGDRPSLTLYSCGGEGIVLQHNPWKLSGEAFDINKLMRDTNSIRYKLPVHTEISWKGDGKVMALGNEDGSIEIFQVPNLKLLCTIQQHHKLVNAIVWHHEHGSRPELSCLLASGSNNAVIYVHNLKAVLESNPESPITITEPYRTLSGHTAKVTSLAWSPHHDGRLVSACYDGTAQVWDALREEPLFNFRGHRGRLLCVAWSPVDPECIYSGADDFCVYRWLTSMQDHSRPPQGKKCIELEKKRLSQFKPKLKKKKKPTLRLPIKQDSSIGNEDESVRENLAPVENGLSDQDGEEEAQEPELPPSPVVCGEPVSCTAVSLGLEKSKVTVTSKVASLKKEPPKEKPEALLKKRKARSMLPLSTSLDHRSKEELHRDCLVLATATHARAELHEDVSADLEERFHLGLFTDRAALYRMMETEGKGHLESGHPELFHQLMLWKGDLKGVLQAAAERGELTDSLVAVAPVAGYSVWLWAVEAFAKQLCFQDQYVKAASYLLSIHRVYEAVELLRSNHLYREAIAVAKARLRPEDPVLKDLYLSWGSILERDGHYAIAAKCYLGATSAYDAAKVLARKGDAASLRTAAELAAIAGESELAASLALRCAQELLLLKNWVGAQEALGLHESLQGQRLVFCLLELLCRHLEEKQPPEVRGPSSIYHQWATGSEGTLVQRVTGVWRSAFSVDTPEQCRAALQKLQDVKYPSATSNTPFRQLLLHVCHDLTLAMLSQQAASWEEAVPALLQAVVRSYTSGNFTLMQEIYSAFLPGGCDHLRDKLGDLSPTMAAFKSLEAFFIYGQLYEVWWSLCGPGSESSIWVWSAESTVSDKQSKPGDSASAEDMEQPLGPGLRLSAESERLLSACKELFSERHASLQTSQRTVAEVQETLAEMIRQHQKSQLCKATANGPNRDEPSQQAERAPSQPPSPTEERNAPVSLPELTRRLTEANERIAEFPESVKAWPFPDVLECCLVLLHIGSQCPGAVNPEMQQQAQKLLQKYGHTRAYRRHCQIRHT

Target ORF information:

RefSeq Version	NM_001172089.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus gem (nuclear organelle) associated protein 5 (Gemin5), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001172089.1

ORF Insert Sequence:

ATGAAACCAG AGCCGCGGAC GCTTCCGCCG TCCCCCAACT GGTACTGCGC GCGCTGCAGC 
GACGCGGCGC CCGGAGGCAT CTTTGGCTTC GCTGCGCGGA CCTCCGTCTT CCTCGTCCGC 
GTGGGCCCGG GCGCCGGCGC GAGCCCAGGG GCGCCCCCGT TTCGAGTTGT AGGAGAGTTG 
GTGGGACACA CTGAAAGAGT ATCTGGTTTC ACTTTTTCTC ATCACCCTGG ACAATACAAC 
CTCTGTGCCA CCAGCTCAGA TGATGGGACT GTGAAGATTT GGGATGTAGA GACCAAGGCT 
GTAGTGACAG AACACGCGCT CCATCAGCAT ACAATATCAG CACTGCATTG GTCTCCGACA 
GTTAAGGACT TAATAGTCTC TGGGGATGAA AAAGGAGTTG TTTTCTGTTA CTGGCTTAAC 
AGAAGTGACA GCCAGCACCT CTTCACAGAG CCCAGGGCCA TATTCTGTCT GACCTGTTCA 
CCTCATCACG AAAATCTAGT AGCCATTGGC TACAAAGACG GCATAGTGAT TATCATTGAC 
ATCAGTAAAA AGGGAGAAGT TGTTCACAGG CTTCGAGGGC ACGACGATGA AATCCACTCC 
ATAGCCTGGT GTCCTTTGTC TGGTGAAGAC TGCTTATCTA TAAACCAAGA GGAAAACTCA 
GAAGAACCTG ACATTCCCAA TGGGAAGCTT ATAGCAGAAA CCCCCATCAC AAAAGGCTGC 
TACTTAGCTA CAGGAAGCAA GGATCAGACC ATTCGAATCT GGAGCTGTTC CCGAGGCCGG 
GGTGTAATGG TTTTGAAATT GCCCTTTCTG AAGAGAAGAA GTGGGGGTGT GGACCCAACA 
GTTAAAGAAC GTCTTTGGTT GACACTTCAT TGGCCCAAGA ACCAACCAAC ACAGCTGGTA 
TCCAGTTGTT TTGGAGGTGA GCTGCTGCTG TGGGACCTCA CCCAGTCATG GAGACGGAAG 
TACACGCTCT TCAGCACCTC AGCGGAAGGG CACAATCACT CCAGAATTGT CTTCAACTTG 
TGTCCTTTGA AGACTGAGGA TGGCAAACAG CTGCTATTGT CCACGTCCAT GGACAGAGAT 
GTAAAATGTT GGGACATGGC CACCCTGGAG TGTTGCTGGA CATTACCTTC TCTTGGAGGT 
TTTGCCTACA GCCTGGCCTT TTCACCTGTG GACGTGGGCT CCCTGGCCAT AGGTGTTGGG 
GACGGCATGA TCCGGGTATG GAATACACTC TCCATAAAGA ACAACTACGA TGTGAAAAAC 
TTCTGGCAAG GAGTCAAGTC CAAGGTCACG GCGCTATGCT GGCATCCAAA CAAGGAGGGC 
TGCTTAGCCT TTGGGACCGA TGACGGAAAA GTGGGATTGT ATGACACCTG CTCCAACAAA 
CCTCCACAGA TTTCAAGCAC ATATCATAAG AAGACCGTAT ATAGGTTGGC TTGGGGGCCA 
CCAGTGCCCC CCATGTCACT TGGAGGGGAA GGAGACAGGC CTTCCCTCAC CTTGTACAGC 
TGTGGAGGGG AAGGCATTGT GCTGCAGCAT AATCCCTGGA AGCTCAGTGG AGAGGCCTTT 
GACATCAACA AACTCATGAG AGACACCAAC TCCATTAGAT ACAAGCTGCC TGTGCACACA 
GAGATCAGCT GGAAAGGAGA TGGCAAAGTC ATGGCTCTTG GAAATGAAGA TGGATCAATA 
GAAATATTCC AAGTCCCAAA CTTGAAACTG CTCTGCACCA TCCAGCAGCA CCACAAGCTT 
GTGAACGCCA TAGTCTGGCA CCATGAGCAT GGCAGCCGTC CAGAGCTGAG CTGCCTCCTT 
GCTTCTGGCT CCAACAATGC AGTTATCTAC GTGCACAACC TGAAAGCTGT CCTAGAGAGC 
AATCCTGAGT CTCCAATAAC CATCACAGAG CCCTACCGGA CCCTCTCAGG GCACACAGCC 
AAGGTTACCA GTCTGGCATG GAGCCCACAT CATGATGGAA GGCTGGTATC TGCTTGCTAC 
GATGGCACAG CTCAGGTGTG GGATGCCCTC CGAGAAGAAC CTTTGTTCAA TTTCCGAGGA 
CATCGAGGCC GGCTGTTATG TGTTGCGTGG TCCCCAGTGG ATCCAGAATG CATCTACTCA 
GGTGCAGATG ACTTCTGTGT CTACAGGTGG CTGACCTCCA TGCAGGACCA TTCCCGGCCT 
CCTCAAGGGA AAAAATGTAT TGAATTAGAG AAAAAACGGC TCTCTCAATT TAAGCCAAAG 
CTAAAAAAGA AGAAAAAACC TACCTTGCGA CTGCCCATAA AGCAAGATTC ATCCATTGGC 
AATGAAGATG AGAGTGTGAG GGAGAACTTG GCACCTGTTG AGAATGGCTT GTCCGATCAG 
GATGGTGAGG AGGAAGCCCA GGAGCCGGAG CTGCCTCCCT CTCCCGTGGT TTGTGGAGAA 
CCTGTTTCCT GCACTGCTGT CTCCTTAGGC TTGGAAAAGT CAAAAGTCAC TGTTACTAGT 
AAAGTCGCCT CACTGAAAAA GGAGCCACCT AAAGAGAAGC CAGAAGCCTT GCTCAAGAAG 
AGGAAGGCTC GCTCCATGCT CCCCCTGAGC ACCAGCTTAG ACCACAGGTC CAAAGAGGAG 
CTCCATCGAG ACTGTTTGGT TCTAGCAACG GCAACACACG CCAGAGCAGA ACTGCATGAA 
GATGTGTCTG CCGACCTTGA GGAACGATTT CACTTGGGCC TTTTCACAGA CAGGGCTGCT 
CTGTACAGGA TGATGGAGAC AGAGGGGAAA GGTCACTTGG AAAGTGGCCA CCCCGAGCTT 
TTTCACCAGC TCATGCTCTG GAAGGGAGAT CTGAAAGGCG TTCTCCAGGC TGCAGCAGAG 
AGAGGCGAGC TCACAGACAG TCTCGTGGCC GTGGCACCAG TAGCTGGCTA CAGTGTGTGG 
CTGTGGGCTG TGGAAGCCTT TGCCAAGCAG CTGTGTTTCC AGGATCAGTA TGTCAAGGCT 
GCCTCCTACC TGCTATCCAT CCACAGAGTG TACGAAGCTG TGGAGCTTCT CAGGTCAAAC 
CATCTCTACA GAGAAGCTAT TGCAGTTGCC AAGGCTCGGC TGCGCCCTGA GGACCCTGTT 
CTGAAGGACC TGTACCTCAG CTGGGGGTCC ATCCTGGAGA GAGATGGCCA CTATGCAATT 
GCAGCCAAGT GCTACTTAGG AGCCACTTCC GCCTATGATG CAGCCAAAGT CCTAGCCAGA 
AAGGGAGATG CAGCCTCTCT TAGAACAGCT GCAGAGCTGG CTGCCATAGC AGGAGAGAGT 
GAGCTGGCTG CTTCCCTGGC TCTTAGGTGC GCCCAAGAGC TGCTTCTGCT GAAGAACTGG 
GTGGGTGCAC AGGAAGCCTT GGGACTGCAT GAAAGCCTGC AGGGCCAGAG ACTGGTGTTC 
TGCCTCCTTG AGCTTCTATG CCGGCACCTG GAGGAAAAAC AGCCTCCAGA GGTCAGAGGC 
CCCTCTTCCA TTTACCACCA GTGGGCCACT GGTTCTGAGG GAACCTTGGT GCAGAGAGTA 
ACTGGTGTGT GGCGCAGCGC CTTCAGTGTG GACACCCCGG AGCAGTGTCG GGCCGCCTTG 
CAGAAGCTAC AGGATGTCAA GTACCCATCA GCAACAAGTA ACACTCCCTT CAGACAGCTG 
CTGCTTCATG TCTGCCATGA CCTAACCTTA GCAATGCTGA GCCAGCAGGC TGCTTCCTGG 
GAGGAGGCTG TCCCTGCACT GCTGCAGGCT GTGGTTCGGA GCTACACCTC AGGGAATTTC 
ACCCTCATGC AAGAAATCTA CTCAGCCTTT CTCCCAGGCG GCTGTGACCA CCTACGAGAC 
AAACTAGGTG ACCTATCTCC TACCATGGCG GCTTTCAAAA GCTTAGAGGC CTTTTTCATT 
TATGGGCAAC TGTATGAAGT CTGGTGGTCA CTCTGTGGGC CTGGCTCAGA ATCAAGCATC 
TGGGTGTGGT CGGCTGAAAG CACAGTCTCT GATAAACAGA GCAAGCCAGG GGACAGTGCC 
AGTGCTGAAG ACATGGAGCA GCCTCTAGGC CCAGGCCTGA GGCTCAGTGC GGAGAGTGAA 
CGTTTGCTGA GTGCCTGCAA GGAGCTCTTC TCAGAGAGGC ATGCCAGCCT ACAGACCTCC 
CAGAGGACTG TTGCTGAAGT CCAAGAGACG CTGGCAGAGA TGATCCGCCA GCACCAAAAG 
AGTCAGCTCT GCAAGGCCAC AGCCAACGGC CCCAACAGAG ATGAACCCAG CCAGCAAGCA 
GAGCGGGCCC CTTCCCAACC TCCGAGCCCA ACAGAAGAAA GAAATGCACC GGTGTCTCTC 
CCTGAATTAA CCAGAAGGCT CACAGAGGCA AACGAGAGAA TAGCTGAGTT TCCAGAGAGT 
GTAAAGGCCT GGCCCTTTCC AGATGTGCTG GAGTGCTGTC TCGTCTTACT TCACATCGGG 
TCCCAGTGTC CTGGTGCTGT GAACCCTGAG ATGCAGCAAC AGGCCCAGAA GCTCCTTCAG 
AAGTATGGCC ACACTAGAGC TTACAGAAGA CACTGCCAGA TTCGACACAC ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gemin5 ( XM_006246396.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006246458.1 Rattus norvegicus gem (nuclear organelle) associated protein 5 (Gemin5), transcript variant X1, mRNA.

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CloneID	ORa33117
Clone ID Related Accession (Same CDS sequence)	XM_006246396.2
Accession Version	XM_006246396.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4497bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	gem-associated protein 5 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005109.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 7, 2014 this sequence version replaced XM_006246396.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAACCAG AGCCGCGGAC GCTTCCGCCG TCCCCCAACT GGTACTGCGC GCGCTGCAGC 
GACGCGGCGC CCGGAGGCAT CTTTGGCTTC GCTGCGCGGA CCTCCGTCTT CCTCGTCCGC 
GTGGGCCCGG GCGCCGGCGC GAGCCCAGGG GCGCCCCCGT TTCGAGTTGT AGGAGAGTTG 
GTGGGACACA CTGAAAGAGT ATCTGGTTTC ACTTTTTCTC ATCACCCTGG ACAATACAAC 
CTCTGTGCCA CCAGCTCAGA TGATGGGACT GTGAAGATTT GGGATGTAGA GACCAAGGCT 
GTAGTGACAG AACACGCGCT CCATCAGCAT ACAATATCAG CACTGCATTG GTCTCCGACA 
GTTAAGGACT TAATAGTCTC TGGGGATGAA AAAGGAGTTG TTTTCTGTTA CTGGCTTAAC 
AGAAGTGACA GCCAGCACCT CTTCACAGAG CCCAGGGCCA TATTCTGTCT GACCTGTTCA 
CCTCATCACG AAAATCTAGT AGCCATTGGC TACAAAGACG GCATAGTGAT TATCATTGAC 
ATCAGTAAAA AGGGAGAAGT TGTTCACAGG CTTCGAGGGC ACGACGATGA AATCCACTCC 
ATAGCCTGGT GTCCTTTGTC TGGTGAAGAC TGCTTATCTA TAAACCAAGA GGAAAACTCA 
GAAGAACCTG ACATTCCCAA TGGGAAGCTT ATAGCAGAAA CCCCCATCAC AAAAGGCTGC 
TACTTAGCTA CAGGAAGCAA GGATCAGACC ATTCGAATCT GGAGCTGTTC CCGAGGCCGG 
GGTGTAATGG TTTTGAAATT GCCCTTTCTG AAGAGAAGAA GTGGGGGTGT GGACCCAACA 
GTTAAAGAAC GTCTTTGGTT GACACTTCAT TGGCCCAAGA ACCAACCAAC ACAGCTGGTA 
TCCAGTTGTT TTGGAGGTGA GCTGCTGCTG TGGGACCTCA CCCAGTCATG GAGACGGAAG 
TACACGCTCT TCAGCACCTC AGCGGAAGGG CACAATCACT CCAGAATTGT CTTCAACTTG 
TGTCCTTTGA AGACTGAGGA TGGCAAACAG CTGCTATTGT CCACGTCCAT GGACAGAGAT 
GTAAAATGTT GGGACATGGC CACCCTGGAG TGTTGCTGGA CATTACCTTC TCTTGGAGGT 
TTTGCCTACA GCCTGGCCTT TTCACCTGTG GACGTGGGCT CCCTGGCCAT AGGTGTTGGG 
GACGGCATGA TCCGGGTATG GAATACACTC TCCATAAAGA ACAACTACGA TGTGAAAAAC 
TTCTGGCAAG GAGTCAAGTC CAAGGTCACG GCGCTATGCT GGCATCCAAA CAAGGAGGGC 
TGCTTAGCCT TTGGGACCGA TGACGGAAAA GTGGGATTGT ATGACACCTG CTCCAACAAA 
CCTCCACAGA TTTCAAGCAC ATATCATAAG AAGACCGTAT ATAGGTTGGC TTGGGGGCCA 
CCAGTGCCCC CCATGTCACT TGGAGGGGAA GGAGACAGGC CTTCCCTCAC CTTGTACAGC 
TGTGGAGGGG AAGGCATTGT GCTGCAGCAT AATCCCTGGA AGCTCAGTGG AGAGGCCTTT 
GACATCAACA AACTCATGAG AGACACCAAC TCCATTAGAT ACAAGCTGCC TGTGCACACA 
GAGATCAGCT GGAAAGGAGA TGGCAAAGTC ATGGCTCTTG GAAATGAAGA TGGATCAATA 
GAAATATTCC AAGTCCCAAA CTTGAAACTG CTCTGCACCA TCCAGCAGCA CCACAAGCTT 
GTGAACGCCA TAGTCTGGCA CCATGAGCAT GGCAGCCGTC CAGAGCTGAG CTGCCTCCTT 
GCTTCTGGCT CCAACAATGC AGTTATCTAC GTGCACAACC TGAAAGCTGT CCTAGAGAGC 
AATCCTGAGT CTCCAATAAC CATCACAGAG CCCTACCGGA CCCTCTCAGG GCACACAGCC 
AAGGTTACCA GTCTGGCATG GAGCCCACAT CATGATGGAA GGCTGGTATC TGCTTGCTAC 
GATGGCACAG CTCAGGTGTG GGATGCCCTC CGAGAAGAAC CTTTGTTCAA TTTCCGAGGA 
CATCGAGGCC GGCTGTTATG TGTTGCGTGG TCCCCAGTGG ATCCAGAATG CATCTACTCA 
GGTGCAGATG ACTTCTGTGT CTACAGGTGG CTGACCTCCA TGCAGGACCA TTCCCGGCCT 
CCTCAAGGGA AAAAATGTAT TGAATTAGAG AAAAAACGGC TCTCTCAATT TAAGCCAAAG 
CTAAAAAAGA AGAAAAAACC TACCTTGCGA CTGCCCATAA AGCAAGATTC ATCCATTGGC 
AATGAAGATG AGAGTGTGAG GGAGAACTTG GCACCTGTTG AGAATGGCTT GTCCGATCAG 
GATGGTGAGG AGGAAGCCCA GGAGCCGGAG CTGCCTCCCT CTCCCGTGGT TTGTGGAGAA 
CCTGTTTCCT GCACTGCTGT CTCCTTAGGC TTGGAAAAGT CAAAAGTCAC TGTTACTAGT 
AAAGTCGCCT CACTGAAAAA GGAGCCACCT AAAGAGAAGC CAGAAGCCTT GCTCAAGAAG 
AGGAAGGCTC GCTCCATGCT CCCCCTGAGC ACCAGCTTAG ACCACAGGTC CAAAGAGGAG 
CTCCATCGAG ACTGTTTGGT TCTAGCAACG GCAACACACG CCAGAGCAGA ACTGCATGAA 
GATGTGTCTG CCGACCTTGA GGAACGATTT CACTTGGGCC TTTTCACAGA CAGGGCTGCT 
CTGTACAGGA TGATGGAGAC AGAGGGGAAA GGTCACTTGG AAAGTGGCCA CCCCGAGCTT 
TTTCACCAGC TCATGCTCTG GAAGGGAGAT CTGAAAGGCG TTCTCCAGGC TGCAGCAGAG 
AGAGGCGAGC TCACAGACAG TCTCGTGGCC GTGGCACCAG TAGCTGGCTA CAGTGTGTGG 
CTGTGGGCTG TGGAAGCCTT TGCCAAGCAG CTGTGTTTCC AGGATCAGTA TGTCAAGGCT 
GCCTCCTACC TGCTATCCAT CCACAGAGTG TACGAAGCTG TGGAGCTTCT CAGGTCAAAC 
CATCTCTACA GAGAAGCTAT TGCAGTTGCC AAGGCTCGGC TGCGCCCTGA GGACCCTGTT 
CTGAAGGACC TGTACCTCAG CTGGGGGTCC ATCCTGGAGA GAGATGGCCA CTATGCAATT 
GCAGCCAAGT GCTACTTAGG AGCCACTTCC GCCTATGATG CAGCCAAAGT CCTAGCCAGA 
AAGGGAGATG CAGCCTCTCT TAGAACAGCT GCAGAGCTGG CTGCCATAGC AGGAGAGAGT 
GAGCTGGCTG CTTCCCTGGC TCTTAGGTGC GCCCAAGAGC TGCTTCTGCT GAAGAACTGG 
GTGGGTGCAC AGGAAGCCTT GGGACTGCAT GAAAGCCTGC AGGGCCAGAG ACTGGTGTTC 
TGCCTCCTTG AGCTTCTATG CCGGCACCTG GAGGAAAAAC AGCCTCCAGA GGTCAGAGGC 
CCCTCTTCCA TTTACCACCA GTGGGCCACT GGTTCTGAGG GAACCTTGGT GCAGAGAGTA 
ACTGGTGTGT GGCGCAGCGC CTTCAGTGTG GACACCCCGG AGCAGTGTCG GGCCGCCTTG 
CAGAAGCTAC AGGATGTCAA GTACCCATCA GCAACAAGTA ACACTCCCTT CAGACAGCTG 
CTGCTTCATG TCTGCCATGA CCTAACCTTA GCAATGCTGA GCCAGCAGGC TGCTTCCTGG 
GAGGAGGCTG TCCCTGCACT GCTGCAGGCT GTGGTTCGGA GCTACACCTC AGGGAATTTC 
ACCCTCATGC AAGAAATCTA CTCAGCCTTT CTCCCAGGCG GCTGTGACCA CCTACGAGAC 
AAACTAGGTG ACCTATCTCC TACCATGGCG GCTTTCAAAA GCTTAGAGGC CTTTTTCATT 
TATGGGCAAC TGTATGAAGT CTGGTGGTCA CTCTGTGGGC CTGGCTCAGA ATCAAGCATC 
TGGGTGTGGT CGGCTGAAAG CACAGTCTCT GATAAACAGA GCAAGCCAGG GGACAGTGCC 
AGTGCTGAAG ACATGGAGCA GCCTCTAGGC CCAGGCCTGA GGCTCAGTGC GGAGAGTGAA 
CGTTTGCTGA GTGCCTGCAA GGAGCTCTTC TCAGAGAGGC ATGCCAGCCT ACAGACCTCC 
CAGAGGACTG TTGCTGAAGT CCAAGAGACG CTGGCAGAGA TGATCCGCCA GCACCAAAAG 
AGTCAGCTCT GCAAGGCCAC AGCCAACGGC CCCAACAGAG ATGAACCCAG CCAGCAAGCA 
GAGCGGGCCC CTTCCCAACC TCCGAGCCCA ACCAGAGAAG AAAGAAATGC ACCGGTGTCT 
CTCCCTGAAT TAACCAGAAG GCTCACAGAG GCAAACGAGA GAATAGCTGA GTTTCCAGAG 
AGTGTAAAGG CCTGGCCCTT TCCAGATGTG CTGGAGTGCT GTCTCGTCTT ACTTCACATC 
GGGTCCCAGT GTCCTGGTGC TGTGAACCCT GAGATGCAGC AACAGGCCCA GAAGCTCCTT 
CAGAAGTATG GCCACACTAG AGCTTACAGA AGACACTGCC AGATTCGACA CACATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006246458.1
CDS	93..4589
Translation	MKPEPRTLPPSPNWYCARCSDAAPGGIFGFAARTSVFLVRVGPGAGASPGAPPFRVVGELVGHTERVSGFTFSHHPGQYNLCATSSDDGTVKIWDVETKAVVTEHALHQHTISALHWSPTVKDLIVSGDEKGVVFCYWLNRSDSQHLFTEPRAIFCLTCSPHHENLVAIGYKDGIVIIIDISKKGEVVHRLRGHDDEIHSIAWCPLSGEDCLSINQEENSEEPDIPNGKLIAETPITKGCYLATGSKDQTIRIWSCSRGRGVMVLKLPFLKRRSGGVDPTVKERLWLTLHWPKNQPTQLVSSCFGGELLLWDLTQSWRRKYTLFSTSAEGHNHSRIVFNLCPLKTEDGKQLLLSTSMDRDVKCWDMATLECCWTLPSLGGFAYSLAFSPVDVGSLAIGVGDGMIRVWNTLSIKNNYDVKNFWQGVKSKVTALCWHPNKEGCLAFGTDDGKVGLYDTCSNKPPQISSTYHKKTVYRLAWGPPVPPMSLGGEGDRPSLTLYSCGGEGIVLQHNPWKLSGEAFDINKLMRDTNSIRYKLPVHTEISWKGDGKVMALGNEDGSIEIFQVPNLKLLCTIQQHHKLVNAIVWHHEHGSRPELSCLLASGSNNAVIYVHNLKAVLESNPESPITITEPYRTLSGHTAKVTSLAWSPHHDGRLVSACYDGTAQVWDALREEPLFNFRGHRGRLLCVAWSPVDPECIYSGADDFCVYRWLTSMQDHSRPPQGKKCIELEKKRLSQFKPKLKKKKKPTLRLPIKQDSSIGNEDESVRENLAPVENGLSDQDGEEEAQEPELPPSPVVCGEPVSCTAVSLGLEKSKVTVTSKVASLKKEPPKEKPEALLKKRKARSMLPLSTSLDHRSKEELHRDCLVLATATHARAELHEDVSADLEERFHLGLFTDRAALYRMMETEGKGHLESGHPELFHQLMLWKGDLKGVLQAAAERGELTDSLVAVAPVAGYSVWLWAVEAFAKQLCFQDQYVKAASYLLSIHRVYEAVELLRSNHLYREAIAVAKARLRPEDPVLKDLYLSWGSILERDGHYAIAAKCYLGATSAYDAAKVLARKGDAASLRTAAELAAIAGESELAASLALRCAQELLLLKNWVGAQEALGLHESLQGQRLVFCLLELLCRHLEEKQPPEVRGPSSIYHQWATGSEGTLVQRVTGVWRSAFSVDTPEQCRAALQKLQDVKYPSATSNTPFRQLLLHVCHDLTLAMLSQQAASWEEAVPALLQAVVRSYTSGNFTLMQEIYSAFLPGGCDHLRDKLGDLSPTMAAFKSLEAFFIYGQLYEVWWSLCGPGSESSIWVWSAESTVSDKQSKPGDSASAEDMEQPLGPGLRLSAESERLLSACKELFSERHASLQTSQRTVAEVQETLAEMIRQHQKSQLCKATANGPNRDEPSQQAERAPSQPPSPTREERNAPVSLPELTRRLTEANERIAEFPESVKAWPFPDVLECCLVLLHIGSQCPGAVNPEMQQQAQKLLQKYGHTRAYRRHCQIRHT

Target ORF information:

RefSeq Version	XM_006246396.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus gem (nuclear organelle) associated protein 5 (Gemin5), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006246396.2

ORF Insert Sequence:

ATGAAACCAG AGCCGCGGAC GCTTCCGCCG TCCCCCAACT GGTACTGCGC GCGCTGCAGC 
GACGCGGCGC CCGGAGGCAT CTTTGGCTTC GCTGCGCGGA CCTCCGTCTT CCTCGTCCGC 
GTGGGCCCGG GCGCCGGCGC GAGCCCAGGG GCGCCCCCGT TTCGAGTTGT AGGAGAGTTG 
GTGGGACACA CTGAAAGAGT ATCTGGTTTC ACTTTTTCTC ATCACCCTGG ACAATACAAC 
CTCTGTGCCA CCAGCTCAGA TGATGGGACT GTGAAGATTT GGGATGTAGA GACCAAGGCT 
GTAGTGACAG AACACGCGCT CCATCAGCAT ACAATATCAG CACTGCATTG GTCTCCGACA 
GTTAAGGACT TAATAGTCTC TGGGGATGAA AAAGGAGTTG TTTTCTGTTA CTGGCTTAAC 
AGAAGTGACA GCCAGCACCT CTTCACAGAG CCCAGGGCCA TATTCTGTCT GACCTGTTCA 
CCTCATCACG AAAATCTAGT AGCCATTGGC TACAAAGACG GCATAGTGAT TATCATTGAC 
ATCAGTAAAA AGGGAGAAGT TGTTCACAGG CTTCGAGGGC ACGACGATGA AATCCACTCC 
ATAGCCTGGT GTCCTTTGTC TGGTGAAGAC TGCTTATCTA TAAACCAAGA GGAAAACTCA 
GAAGAACCTG ACATTCCCAA TGGGAAGCTT ATAGCAGAAA CCCCCATCAC AAAAGGCTGC 
TACTTAGCTA CAGGAAGCAA GGATCAGACC ATTCGAATCT GGAGCTGTTC CCGAGGCCGG 
GGTGTAATGG TTTTGAAATT GCCCTTTCTG AAGAGAAGAA GTGGGGGTGT GGACCCAACA 
GTTAAAGAAC GTCTTTGGTT GACACTTCAT TGGCCCAAGA ACCAACCAAC ACAGCTGGTA 
TCCAGTTGTT TTGGAGGTGA GCTGCTGCTG TGGGACCTCA CCCAGTCATG GAGACGGAAG 
TACACGCTCT TCAGCACCTC AGCGGAAGGG CACAATCACT CCAGAATTGT CTTCAACTTG 
TGTCCTTTGA AGACTGAGGA TGGCAAACAG CTGCTATTGT CCACGTCCAT GGACAGAGAT 
GTAAAATGTT GGGACATGGC CACCCTGGAG TGTTGCTGGA CATTACCTTC TCTTGGAGGT 
TTTGCCTACA GCCTGGCCTT TTCACCTGTG GACGTGGGCT CCCTGGCCAT AGGTGTTGGG 
GACGGCATGA TCCGGGTATG GAATACACTC TCCATAAAGA ACAACTACGA TGTGAAAAAC 
TTCTGGCAAG GAGTCAAGTC CAAGGTCACG GCGCTATGCT GGCATCCAAA CAAGGAGGGC 
TGCTTAGCCT TTGGGACCGA TGACGGAAAA GTGGGATTGT ATGACACCTG CTCCAACAAA 
CCTCCACAGA TTTCAAGCAC ATATCATAAG AAGACCGTAT ATAGGTTGGC TTGGGGGCCA 
CCAGTGCCCC CCATGTCACT TGGAGGGGAA GGAGACAGGC CTTCCCTCAC CTTGTACAGC 
TGTGGAGGGG AAGGCATTGT GCTGCAGCAT AATCCCTGGA AGCTCAGTGG AGAGGCCTTT 
GACATCAACA AACTCATGAG AGACACCAAC TCCATTAGAT ACAAGCTGCC TGTGCACACA 
GAGATCAGCT GGAAAGGAGA TGGCAAAGTC ATGGCTCTTG GAAATGAAGA TGGATCAATA 
GAAATATTCC AAGTCCCAAA CTTGAAACTG CTCTGCACCA TCCAGCAGCA CCACAAGCTT 
GTGAACGCCA TAGTCTGGCA CCATGAGCAT GGCAGCCGTC CAGAGCTGAG CTGCCTCCTT 
GCTTCTGGCT CCAACAATGC AGTTATCTAC GTGCACAACC TGAAAGCTGT CCTAGAGAGC 
AATCCTGAGT CTCCAATAAC CATCACAGAG CCCTACCGGA CCCTCTCAGG GCACACAGCC 
AAGGTTACCA GTCTGGCATG GAGCCCACAT CATGATGGAA GGCTGGTATC TGCTTGCTAC 
GATGGCACAG CTCAGGTGTG GGATGCCCTC CGAGAAGAAC CTTTGTTCAA TTTCCGAGGA 
CATCGAGGCC GGCTGTTATG TGTTGCGTGG TCCCCAGTGG ATCCAGAATG CATCTACTCA 
GGTGCAGATG ACTTCTGTGT CTACAGGTGG CTGACCTCCA TGCAGGACCA TTCCCGGCCT 
CCTCAAGGGA AAAAATGTAT TGAATTAGAG AAAAAACGGC TCTCTCAATT TAAGCCAAAG 
CTAAAAAAGA AGAAAAAACC TACCTTGCGA CTGCCCATAA AGCAAGATTC ATCCATTGGC 
AATGAAGATG AGAGTGTGAG GGAGAACTTG GCACCTGTTG AGAATGGCTT GTCCGATCAG 
GATGGTGAGG AGGAAGCCCA GGAGCCGGAG CTGCCTCCCT CTCCCGTGGT TTGTGGAGAA 
CCTGTTTCCT GCACTGCTGT CTCCTTAGGC TTGGAAAAGT CAAAAGTCAC TGTTACTAGT 
AAAGTCGCCT CACTGAAAAA GGAGCCACCT AAAGAGAAGC CAGAAGCCTT GCTCAAGAAG 
AGGAAGGCTC GCTCCATGCT CCCCCTGAGC ACCAGCTTAG ACCACAGGTC CAAAGAGGAG 
CTCCATCGAG ACTGTTTGGT TCTAGCAACG GCAACACACG CCAGAGCAGA ACTGCATGAA 
GATGTGTCTG CCGACCTTGA GGAACGATTT CACTTGGGCC TTTTCACAGA CAGGGCTGCT 
CTGTACAGGA TGATGGAGAC AGAGGGGAAA GGTCACTTGG AAAGTGGCCA CCCCGAGCTT 
TTTCACCAGC TCATGCTCTG GAAGGGAGAT CTGAAAGGCG TTCTCCAGGC TGCAGCAGAG 
AGAGGCGAGC TCACAGACAG TCTCGTGGCC GTGGCACCAG TAGCTGGCTA CAGTGTGTGG 
CTGTGGGCTG TGGAAGCCTT TGCCAAGCAG CTGTGTTTCC AGGATCAGTA TGTCAAGGCT 
GCCTCCTACC TGCTATCCAT CCACAGAGTG TACGAAGCTG TGGAGCTTCT CAGGTCAAAC 
CATCTCTACA GAGAAGCTAT TGCAGTTGCC AAGGCTCGGC TGCGCCCTGA GGACCCTGTT 
CTGAAGGACC TGTACCTCAG CTGGGGGTCC ATCCTGGAGA GAGATGGCCA CTATGCAATT 
GCAGCCAAGT GCTACTTAGG AGCCACTTCC GCCTATGATG CAGCCAAAGT CCTAGCCAGA 
AAGGGAGATG CAGCCTCTCT TAGAACAGCT GCAGAGCTGG CTGCCATAGC AGGAGAGAGT 
GAGCTGGCTG CTTCCCTGGC TCTTAGGTGC GCCCAAGAGC TGCTTCTGCT GAAGAACTGG 
GTGGGTGCAC AGGAAGCCTT GGGACTGCAT GAAAGCCTGC AGGGCCAGAG ACTGGTGTTC 
TGCCTCCTTG AGCTTCTATG CCGGCACCTG GAGGAAAAAC AGCCTCCAGA GGTCAGAGGC 
CCCTCTTCCA TTTACCACCA GTGGGCCACT GGTTCTGAGG GAACCTTGGT GCAGAGAGTA 
ACTGGTGTGT GGCGCAGCGC CTTCAGTGTG GACACCCCGG AGCAGTGTCG GGCCGCCTTG 
CAGAAGCTAC AGGATGTCAA GTACCCATCA GCAACAAGTA ACACTCCCTT CAGACAGCTG 
CTGCTTCATG TCTGCCATGA CCTAACCTTA GCAATGCTGA GCCAGCAGGC TGCTTCCTGG 
GAGGAGGCTG TCCCTGCACT GCTGCAGGCT GTGGTTCGGA GCTACACCTC AGGGAATTTC 
ACCCTCATGC AAGAAATCTA CTCAGCCTTT CTCCCAGGCG GCTGTGACCA CCTACGAGAC 
AAACTAGGTG ACCTATCTCC TACCATGGCG GCTTTCAAAA GCTTAGAGGC CTTTTTCATT 
TATGGGCAAC TGTATGAAGT CTGGTGGTCA CTCTGTGGGC CTGGCTCAGA ATCAAGCATC 
TGGGTGTGGT CGGCTGAAAG CACAGTCTCT GATAAACAGA GCAAGCCAGG GGACAGTGCC 
AGTGCTGAAG ACATGGAGCA GCCTCTAGGC CCAGGCCTGA GGCTCAGTGC GGAGAGTGAA 
CGTTTGCTGA GTGCCTGCAA GGAGCTCTTC TCAGAGAGGC ATGCCAGCCT ACAGACCTCC 
CAGAGGACTG TTGCTGAAGT CCAAGAGACG CTGGCAGAGA TGATCCGCCA GCACCAAAAG 
AGTCAGCTCT GCAAGGCCAC AGCCAACGGC CCCAACAGAG ATGAACCCAG CCAGCAAGCA 
GAGCGGGCCC CTTCCCAACC TCCGAGCCCA ACCAGAGAAG AAAGAAATGC ACCGGTGTCT 
CTCCCTGAAT TAACCAGAAG GCTCACAGAG GCAAACGAGA GAATAGCTGA GTTTCCAGAG 
AGTGTAAAGG CCTGGCCCTT TCCAGATGTG CTGGAGTGCT GTCTCGTCTT ACTTCACATC 
GGGTCCCAGT GTCCTGGTGC TGTGAACCCT GAGATGCAGC AACAGGCCCA GAAGCTCCTT 
CAGAAGTATG GCCACACTAG AGCTTACAGA AGACACTGCC AGATTCGACA CACATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gemin5 ( XM_006246397.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006246459.1 Rattus norvegicus gem (nuclear organelle) associated protein 5 (Gemin5), transcript variant X2, mRNA.

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CloneID	ORa33118
Clone ID Related Accession (Same CDS sequence)	XM_006246397.2
Accession Version	XM_006246397.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4494bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	gem-associated protein 5 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005109.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 7, 2014 this sequence version replaced XM_006246397.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAACCAG AGCCGCGGAC GCTTCCGCCG TCCCCCAACT GGTACTGCGC GCGCTGCAGC 
GACGCGGCGC CCGGAGGCAT CTTTGGCTTC GCTGCGCGGA CCTCCGTCTT CCTCGTCCGC 
GTGGGCCCGG GCGCCGGCGC GAGCCCAGGG GCGCCCCCGT TTCGAGTTGT AGGAGAGTTG 
GTGGGACACA CTGAAAGAGT ATCTGGTTTC ACTTTTTCTC ATCACCCTGG ACAATACAAC 
CTCTGTGCCA CCAGCTCAGA TGATGGGACT GTGAAGATTT GGGATGTAGA GACCAAGGCT 
GTAGTGACAG AACACGCGCT CCATCAGCAT ACAATATCAG CACTGCATTG GTCTCCGACA 
GTTAAGGACT TAATAGTCTC TGGGGATGAA AAAGGAGTTG TTTTCTGTTA CTGGCTTAAC 
AGAAGTGACA GCCAGCACCT CTTCACAGAG CCCAGGGCCA TATTCTGTCT GACCTGTTCA 
CCTCATCACG AAAATCTAGT AGCCATTGGC TACAAAGACG GCATAGTGAT TATCATTGAC 
ATCAGTAAAA AGGGAGAAGT TGTTCACAGG CTTCGAGGGC ACGACGATGA AATCCACTCC 
ATAGCCTGGT GTCCTTTGTC TGGTGAAGAC TGCTTATCTA TAAACCAAGA GGAAAACTCA 
GAAGAACCTG ACATTCCCAA TGGGAAGCTT ATAGCAGAAA CCCCCATCAC AAAAGGCTGC 
TACTTAGCTA CAGGAAGCAA GGATCAGACC ATTCGAATCT GGAGCTGTTC CCGAGGCCGG 
GGTGTAATGG TTTTGAAATT GCCCTTTCTG AAGAGAAGAA GTGGGGGTGT GGACCCAACA 
GTTAAAGAAC GTCTTTGGTT GACACTTCAT TGGCCCAAGA ACCAACCAAC ACAGCTGGTA 
TCCAGTTGTT TTGGAGGTGA GCTGCTGCTG TGGGACCTCA CCCAGTCATG GAGACGGAAG 
TACACGCTCT TCAGCACCTC AGCGGAAGGG CACAATCACT CCAGAATTGT CTTCAACTTG 
TGTCCTTTGA AGACTGAGGA TGGCAAACAG CTGCTATTGT CCACGTCCAT GGACAGAGAT 
GTAAAATGTT GGGACATGGC CACCCTGGAG TGTTGCTGGA CATTACCTTC TCTTGGAGGT 
TTTGCCTACA GCCTGGCCTT TTCACCTGTG GACGTGGGCT CCCTGGCCAT AGGTGTTGGG 
GACGGCATGA TCCGGGTATG GAATACACTC TCCATAAAGA ACAACTACGA TGTGAAAAAC 
TTCTGGCAAG GAGTCAAGTC CAAGGTCACG GCGCTATGCT GGCATCCAAA CAAGGAGGGC 
TGCTTAGCCT TTGGGACCGA TGACGGAAAA GTGGGATTGT ATGACACCTG CTCCAACAAA 
CCTCCACAGA TTTCAAGCAC ATATCATAAG AAGACCGTAT ATAGGTTGGC TTGGGGGCCA 
CCAGTGCCCC CCATGTCACT TGGAGGGGAA GGAGACAGGC CTTCCCTCAC CTTGTACAGC 
TGTGGAGGGG AAGGCATTGT GCTGCAGCAT AATCCCTGGA AGCTCAGTGG AGAGGCCTTT 
GACATCAACA AACTCATGAG AGACACCAAC TCCATTAGAT ACAAGCTGCC TGTGCACACA 
GAGATCAGCT GGAAAGGAGA TGGCAAAGTC ATGGCTCTTG GAAATGAAGA TGGATCAATA 
GAAATATTCC AAGTCCCAAA CTTGAAACTG CTCTGCACCA TCCAGCAGCA CCACAAGCTT 
GTGAACGCCA TAGTCTGGCA CCATGAGCAT GGCAGCCGTC CAGAGCTGAG CTGCCTCCTT 
GCTTCTGGCT CCAACAATGC AGTTATCTAC GTGCACAACC TGAAAGCTGT CCTAGAGAGC 
AATCCTGAGT CTCCAATAAC CATCACAGAG CCCTACCGGA CCCTCTCAGG GCACACAGCC 
AAGGTTACCA GTCTGGCATG GAGCCCACAT CATGATGGAA GGCTGGTATC TGCTTGCTAC 
GATGGCACAG CTCAGGTGTG GGATGCCCTC CGAGAAGAAC CTTTGTTCAA TTTCCGAGGA 
CATCGAGGCC GGCTGTTATG TGTTGCGTGG TCCCCAGTGG ATCCAGAATG CATCTACTCA 
GGTGCAGATG ACTTCTGTGT CTACAGGTGG CTGACCTCCA TGCAGGACCA TTCCCGGCCT 
CCTCAAGGGA AAAAATGTAT TGAATTAGAG AAAAAACGGC TCTCTCAATT TAAGCCAAAG 
CTAAAAAAGA AGAAAAAACC TACCTTGCGA CTGCCCATAA AGCAAGATTC ATCCATTGGC 
AATGAAGATG AGAGTGTGAG GGAGAACTTG GCACCTGTTG AGAATGGCTT GTCCGATCAG 
GATGGTGAGG AGGAAGCCCA GGAGCCGGAG CTGCCTCCCT CTCCCGTGGT TTGTGGAGAA 
CCTGTTTCCT GCACTGCTGT CTCCTTAGGC TTGGAAAAGT CAAAAGTCAC TGTTACTAGT 
AAAGTCGCCT CACTGAAAAA GGAGCCACCT AAAGAGAAGC CAGAAGCCTT GCTCAAGAAG 
AGGAAGGCTC GCTCCATGCT CCCCCTGAGC ACCAGCTTAG ACCACAGGTC CAAAGAGGAG 
CTCCATCGAG ACTGTTTGGT TCTAGCAACG GCAACACACG CCAGAGAACT GCATGAAGAT 
GTGTCTGCCG ACCTTGAGGA ACGATTTCAC TTGGGCCTTT TCACAGACAG GGCTGCTCTG 
TACAGGATGA TGGAGACAGA GGGGAAAGGT CACTTGGAAA GTGGCCACCC CGAGCTTTTT 
CACCAGCTCA TGCTCTGGAA GGGAGATCTG AAAGGCGTTC TCCAGGCTGC AGCAGAGAGA 
GGCGAGCTCA CAGACAGTCT CGTGGCCGTG GCACCAGTAG CTGGCTACAG TGTGTGGCTG 
TGGGCTGTGG AAGCCTTTGC CAAGCAGCTG TGTTTCCAGG ATCAGTATGT CAAGGCTGCC 
TCCTACCTGC TATCCATCCA CAGAGTGTAC GAAGCTGTGG AGCTTCTCAG GTCAAACCAT 
CTCTACAGAG AAGCTATTGC AGTTGCCAAG GCTCGGCTGC GCCCTGAGGA CCCTGTTCTG 
AAGGACCTGT ACCTCAGCTG GGGGTCCATC CTGGAGAGAG ATGGCCACTA TGCAATTGCA 
GCCAAGTGCT ACTTAGGAGC CACTTCCGCC TATGATGCAG CCAAAGTCCT AGCCAGAAAG 
GGAGATGCAG CCTCTCTTAG AACAGCTGCA GAGCTGGCTG CCATAGCAGG AGAGAGTGAG 
CTGGCTGCTT CCCTGGCTCT TAGGTGCGCC CAAGAGCTGC TTCTGCTGAA GAACTGGGTG 
GGTGCACAGG AAGCCTTGGG ACTGCATGAA AGCCTGCAGG GCCAGAGACT GGTGTTCTGC 
CTCCTTGAGC TTCTATGCCG GCACCTGGAG GAAAAACAGC CTCCAGAGGT CAGAGGCCCC 
TCTTCCATTT ACCACCAGTG GGCCACTGGT TCTGAGGGAA CCTTGGTGCA GAGAGTAACT 
GGTGTGTGGC GCAGCGCCTT CAGTGTGGAC ACCCCGGAGC AGTGTCGGGC CGCCTTGCAG 
AAGCTACAGG ATGTCAAGTA CCCATCAGCA ACAAGTAACA CTCCCTTCAG ACAGCTGCTG 
CTTCATGTCT GCCATGACCT AACCTTAGCA ATGCTGAGCC AGCAGGCTGC TTCCTGGGAG 
GAGGCTGTCC CTGCACTGCT GCAGGCTGTG GTTCGGAGCT ACACCTCAGG GAATTTCACC 
CTCATGCAAG AAATCTACTC AGCCTTTCTC CCAGGCGGCT GTGACCACCT ACGAGACAAA 
CTAGGTGACC TATCTCCTAC CATGGCGGCT TTCAAAAGCT TAGAGGCCTT TTTCATTTAT 
GGGCAACTGT ATGAAGTCTG GTGGTCACTC TGTGGGCCTG GCTCAGAATC AAGCATCTGG 
GTGTGGTCGG CTGAAAGCAC AGTCTCTGAT AAACAGAGCA AGCCAGGGGA CAGTGCCAGT 
GCTGAAGACA TGGAGCAGCC TCTAGGCCCA GGCCTGAGGC TCAGTGCGGA GAGTGAACGT 
TTGCTGAGTG CCTGCAAGGA GCTCTTCTCA GAGAGGCATG CCAGCCTACA GACCTCCCAG 
AGGACTGTTG CTGAAGTCCA AGAGACGCTG GCAGAGATGA TCCGCCAGCA CCAAAAGAGT 
CAGCTCTGCA AGGCCACAGC CAACGGCCCC AACAGAGATG AACCCAGCCA GCAAGCAGAG 
CGGGCCCCTT CCCAACCTCC GAGCCCAACC AGAGAAGAAA GAAATGCACC GGTGTCTCTC 
CCTGAATTAA CCAGAAGGCT CACAGAGGCA AACGAGAGAA TAGCTGAGTT TCCAGAGAGT 
GTAAAGGCCT GGCCCTTTCC AGATGTGCTG GAGTGCTGTC TCGTCTTACT TCACATCGGG 
TCCCAGTGTC CTGGTGCTGT GAACCCTGAG ATGCAGCAAC AGGCCCAGAA GCTCCTTCAG 
AAGTATGGCC ACACTAGAGC TTACAGAAGA CACTGCCAGA TTCGACACAC ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006246459.1
CDS	93..4586
Translation	MKPEPRTLPPSPNWYCARCSDAAPGGIFGFAARTSVFLVRVGPGAGASPGAPPFRVVGELVGHTERVSGFTFSHHPGQYNLCATSSDDGTVKIWDVETKAVVTEHALHQHTISALHWSPTVKDLIVSGDEKGVVFCYWLNRSDSQHLFTEPRAIFCLTCSPHHENLVAIGYKDGIVIIIDISKKGEVVHRLRGHDDEIHSIAWCPLSGEDCLSINQEENSEEPDIPNGKLIAETPITKGCYLATGSKDQTIRIWSCSRGRGVMVLKLPFLKRRSGGVDPTVKERLWLTLHWPKNQPTQLVSSCFGGELLLWDLTQSWRRKYTLFSTSAEGHNHSRIVFNLCPLKTEDGKQLLLSTSMDRDVKCWDMATLECCWTLPSLGGFAYSLAFSPVDVGSLAIGVGDGMIRVWNTLSIKNNYDVKNFWQGVKSKVTALCWHPNKEGCLAFGTDDGKVGLYDTCSNKPPQISSTYHKKTVYRLAWGPPVPPMSLGGEGDRPSLTLYSCGGEGIVLQHNPWKLSGEAFDINKLMRDTNSIRYKLPVHTEISWKGDGKVMALGNEDGSIEIFQVPNLKLLCTIQQHHKLVNAIVWHHEHGSRPELSCLLASGSNNAVIYVHNLKAVLESNPESPITITEPYRTLSGHTAKVTSLAWSPHHDGRLVSACYDGTAQVWDALREEPLFNFRGHRGRLLCVAWSPVDPECIYSGADDFCVYRWLTSMQDHSRPPQGKKCIELEKKRLSQFKPKLKKKKKPTLRLPIKQDSSIGNEDESVRENLAPVENGLSDQDGEEEAQEPELPPSPVVCGEPVSCTAVSLGLEKSKVTVTSKVASLKKEPPKEKPEALLKKRKARSMLPLSTSLDHRSKEELHRDCLVLATATHARELHEDVSADLEERFHLGLFTDRAALYRMMETEGKGHLESGHPELFHQLMLWKGDLKGVLQAAAERGELTDSLVAVAPVAGYSVWLWAVEAFAKQLCFQDQYVKAASYLLSIHRVYEAVELLRSNHLYREAIAVAKARLRPEDPVLKDLYLSWGSILERDGHYAIAAKCYLGATSAYDAAKVLARKGDAASLRTAAELAAIAGESELAASLALRCAQELLLLKNWVGAQEALGLHESLQGQRLVFCLLELLCRHLEEKQPPEVRGPSSIYHQWATGSEGTLVQRVTGVWRSAFSVDTPEQCRAALQKLQDVKYPSATSNTPFRQLLLHVCHDLTLAMLSQQAASWEEAVPALLQAVVRSYTSGNFTLMQEIYSAFLPGGCDHLRDKLGDLSPTMAAFKSLEAFFIYGQLYEVWWSLCGPGSESSIWVWSAESTVSDKQSKPGDSASAEDMEQPLGPGLRLSAESERLLSACKELFSERHASLQTSQRTVAEVQETLAEMIRQHQKSQLCKATANGPNRDEPSQQAERAPSQPPSPTREERNAPVSLPELTRRLTEANERIAEFPESVKAWPFPDVLECCLVLLHIGSQCPGAVNPEMQQQAQKLLQKYGHTRAYRRHCQIRHT

Target ORF information:

RefSeq Version	XM_006246397.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus gem (nuclear organelle) associated protein 5 (Gemin5), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006246397.2

ORF Insert Sequence:

ATGAAACCAG AGCCGCGGAC GCTTCCGCCG TCCCCCAACT GGTACTGCGC GCGCTGCAGC 
GACGCGGCGC CCGGAGGCAT CTTTGGCTTC GCTGCGCGGA CCTCCGTCTT CCTCGTCCGC 
GTGGGCCCGG GCGCCGGCGC GAGCCCAGGG GCGCCCCCGT TTCGAGTTGT AGGAGAGTTG 
GTGGGACACA CTGAAAGAGT ATCTGGTTTC ACTTTTTCTC ATCACCCTGG ACAATACAAC 
CTCTGTGCCA CCAGCTCAGA TGATGGGACT GTGAAGATTT GGGATGTAGA GACCAAGGCT 
GTAGTGACAG AACACGCGCT CCATCAGCAT ACAATATCAG CACTGCATTG GTCTCCGACA 
GTTAAGGACT TAATAGTCTC TGGGGATGAA AAAGGAGTTG TTTTCTGTTA CTGGCTTAAC 
AGAAGTGACA GCCAGCACCT CTTCACAGAG CCCAGGGCCA TATTCTGTCT GACCTGTTCA 
CCTCATCACG AAAATCTAGT AGCCATTGGC TACAAAGACG GCATAGTGAT TATCATTGAC 
ATCAGTAAAA AGGGAGAAGT TGTTCACAGG CTTCGAGGGC ACGACGATGA AATCCACTCC 
ATAGCCTGGT GTCCTTTGTC TGGTGAAGAC TGCTTATCTA TAAACCAAGA GGAAAACTCA 
GAAGAACCTG ACATTCCCAA TGGGAAGCTT ATAGCAGAAA CCCCCATCAC AAAAGGCTGC 
TACTTAGCTA CAGGAAGCAA GGATCAGACC ATTCGAATCT GGAGCTGTTC CCGAGGCCGG 
GGTGTAATGG TTTTGAAATT GCCCTTTCTG AAGAGAAGAA GTGGGGGTGT GGACCCAACA 
GTTAAAGAAC GTCTTTGGTT GACACTTCAT TGGCCCAAGA ACCAACCAAC ACAGCTGGTA 
TCCAGTTGTT TTGGAGGTGA GCTGCTGCTG TGGGACCTCA CCCAGTCATG GAGACGGAAG 
TACACGCTCT TCAGCACCTC AGCGGAAGGG CACAATCACT CCAGAATTGT CTTCAACTTG 
TGTCCTTTGA AGACTGAGGA TGGCAAACAG CTGCTATTGT CCACGTCCAT GGACAGAGAT 
GTAAAATGTT GGGACATGGC CACCCTGGAG TGTTGCTGGA CATTACCTTC TCTTGGAGGT 
TTTGCCTACA GCCTGGCCTT TTCACCTGTG GACGTGGGCT CCCTGGCCAT AGGTGTTGGG 
GACGGCATGA TCCGGGTATG GAATACACTC TCCATAAAGA ACAACTACGA TGTGAAAAAC 
TTCTGGCAAG GAGTCAAGTC CAAGGTCACG GCGCTATGCT GGCATCCAAA CAAGGAGGGC 
TGCTTAGCCT TTGGGACCGA TGACGGAAAA GTGGGATTGT ATGACACCTG CTCCAACAAA 
CCTCCACAGA TTTCAAGCAC ATATCATAAG AAGACCGTAT ATAGGTTGGC TTGGGGGCCA 
CCAGTGCCCC CCATGTCACT TGGAGGGGAA GGAGACAGGC CTTCCCTCAC CTTGTACAGC 
TGTGGAGGGG AAGGCATTGT GCTGCAGCAT AATCCCTGGA AGCTCAGTGG AGAGGCCTTT 
GACATCAACA AACTCATGAG AGACACCAAC TCCATTAGAT ACAAGCTGCC TGTGCACACA 
GAGATCAGCT GGAAAGGAGA TGGCAAAGTC ATGGCTCTTG GAAATGAAGA TGGATCAATA 
GAAATATTCC AAGTCCCAAA CTTGAAACTG CTCTGCACCA TCCAGCAGCA CCACAAGCTT 
GTGAACGCCA TAGTCTGGCA CCATGAGCAT GGCAGCCGTC CAGAGCTGAG CTGCCTCCTT 
GCTTCTGGCT CCAACAATGC AGTTATCTAC GTGCACAACC TGAAAGCTGT CCTAGAGAGC 
AATCCTGAGT CTCCAATAAC CATCACAGAG CCCTACCGGA CCCTCTCAGG GCACACAGCC 
AAGGTTACCA GTCTGGCATG GAGCCCACAT CATGATGGAA GGCTGGTATC TGCTTGCTAC 
GATGGCACAG CTCAGGTGTG GGATGCCCTC CGAGAAGAAC CTTTGTTCAA TTTCCGAGGA 
CATCGAGGCC GGCTGTTATG TGTTGCGTGG TCCCCAGTGG ATCCAGAATG CATCTACTCA 
GGTGCAGATG ACTTCTGTGT CTACAGGTGG CTGACCTCCA TGCAGGACCA TTCCCGGCCT 
CCTCAAGGGA AAAAATGTAT TGAATTAGAG AAAAAACGGC TCTCTCAATT TAAGCCAAAG 
CTAAAAAAGA AGAAAAAACC TACCTTGCGA CTGCCCATAA AGCAAGATTC ATCCATTGGC 
AATGAAGATG AGAGTGTGAG GGAGAACTTG GCACCTGTTG AGAATGGCTT GTCCGATCAG 
GATGGTGAGG AGGAAGCCCA GGAGCCGGAG CTGCCTCCCT CTCCCGTGGT TTGTGGAGAA 
CCTGTTTCCT GCACTGCTGT CTCCTTAGGC TTGGAAAAGT CAAAAGTCAC TGTTACTAGT 
AAAGTCGCCT CACTGAAAAA GGAGCCACCT AAAGAGAAGC CAGAAGCCTT GCTCAAGAAG 
AGGAAGGCTC GCTCCATGCT CCCCCTGAGC ACCAGCTTAG ACCACAGGTC CAAAGAGGAG 
CTCCATCGAG ACTGTTTGGT TCTAGCAACG GCAACACACG CCAGAGAACT GCATGAAGAT 
GTGTCTGCCG ACCTTGAGGA ACGATTTCAC TTGGGCCTTT TCACAGACAG GGCTGCTCTG 
TACAGGATGA TGGAGACAGA GGGGAAAGGT CACTTGGAAA GTGGCCACCC CGAGCTTTTT 
CACCAGCTCA TGCTCTGGAA GGGAGATCTG AAAGGCGTTC TCCAGGCTGC AGCAGAGAGA 
GGCGAGCTCA CAGACAGTCT CGTGGCCGTG GCACCAGTAG CTGGCTACAG TGTGTGGCTG 
TGGGCTGTGG AAGCCTTTGC CAAGCAGCTG TGTTTCCAGG ATCAGTATGT CAAGGCTGCC 
TCCTACCTGC TATCCATCCA CAGAGTGTAC GAAGCTGTGG AGCTTCTCAG GTCAAACCAT 
CTCTACAGAG AAGCTATTGC AGTTGCCAAG GCTCGGCTGC GCCCTGAGGA CCCTGTTCTG 
AAGGACCTGT ACCTCAGCTG GGGGTCCATC CTGGAGAGAG ATGGCCACTA TGCAATTGCA 
GCCAAGTGCT ACTTAGGAGC CACTTCCGCC TATGATGCAG CCAAAGTCCT AGCCAGAAAG 
GGAGATGCAG CCTCTCTTAG AACAGCTGCA GAGCTGGCTG CCATAGCAGG AGAGAGTGAG 
CTGGCTGCTT CCCTGGCTCT TAGGTGCGCC CAAGAGCTGC TTCTGCTGAA GAACTGGGTG 
GGTGCACAGG AAGCCTTGGG ACTGCATGAA AGCCTGCAGG GCCAGAGACT GGTGTTCTGC 
CTCCTTGAGC TTCTATGCCG GCACCTGGAG GAAAAACAGC CTCCAGAGGT CAGAGGCCCC 
TCTTCCATTT ACCACCAGTG GGCCACTGGT TCTGAGGGAA CCTTGGTGCA GAGAGTAACT 
GGTGTGTGGC GCAGCGCCTT CAGTGTGGAC ACCCCGGAGC AGTGTCGGGC CGCCTTGCAG 
AAGCTACAGG ATGTCAAGTA CCCATCAGCA ACAAGTAACA CTCCCTTCAG ACAGCTGCTG 
CTTCATGTCT GCCATGACCT AACCTTAGCA ATGCTGAGCC AGCAGGCTGC TTCCTGGGAG 
GAGGCTGTCC CTGCACTGCT GCAGGCTGTG GTTCGGAGCT ACACCTCAGG GAATTTCACC 
CTCATGCAAG AAATCTACTC AGCCTTTCTC CCAGGCGGCT GTGACCACCT ACGAGACAAA 
CTAGGTGACC TATCTCCTAC CATGGCGGCT TTCAAAAGCT TAGAGGCCTT TTTCATTTAT 
GGGCAACTGT ATGAAGTCTG GTGGTCACTC TGTGGGCCTG GCTCAGAATC AAGCATCTGG 
GTGTGGTCGG CTGAAAGCAC AGTCTCTGAT AAACAGAGCA AGCCAGGGGA CAGTGCCAGT 
GCTGAAGACA TGGAGCAGCC TCTAGGCCCA GGCCTGAGGC TCAGTGCGGA GAGTGAACGT 
TTGCTGAGTG CCTGCAAGGA GCTCTTCTCA GAGAGGCATG CCAGCCTACA GACCTCCCAG 
AGGACTGTTG CTGAAGTCCA AGAGACGCTG GCAGAGATGA TCCGCCAGCA CCAAAAGAGT 
CAGCTCTGCA AGGCCACAGC CAACGGCCCC AACAGAGATG AACCCAGCCA GCAAGCAGAG 
CGGGCCCCTT CCCAACCTCC GAGCCCAACC AGAGAAGAAA GAAATGCACC GGTGTCTCTC 
CCTGAATTAA CCAGAAGGCT CACAGAGGCA AACGAGAGAA TAGCTGAGTT TCCAGAGAGT 
GTAAAGGCCT GGCCCTTTCC AGATGTGCTG GAGTGCTGTC TCGTCTTACT TCACATCGGG 
TCCCAGTGTC CTGGTGCTGT GAACCCTGAG ATGCAGCAAC AGGCCCAGAA GCTCCTTCAG 
AAGTATGGCC ACACTAGAGC TTACAGAAGA CACTGCCAGA TTCGACACAC ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gemin5 ( XM_006246398.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006246460.1 Rattus norvegicus gem (nuclear organelle) associated protein 5 (Gemin5), transcript variant X3, mRNA.

Price & Availability↑

CloneID	ORa33119
Clone ID Related Accession (Same CDS sequence)	XM_006246398.2
Accession Version	XM_006246398.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4494bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	gem-associated protein 5 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005109.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 7, 2014 this sequence version replaced XM_006246398.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAACCAG AGCCGCGGAC GCTTCCGCCG TCCCCCAACT GGTACTGCGC GCGCTGCAGC 
GACGCGGCGC CCGGAGGCAT CTTTGGCTTC GCTGCGCGGA CCTCCGTCTT CCTCGTCCGC 
GTGGGCCCGG GCGCCGGCGC GAGCCCAGGG GCGCCCCCGT TTCGAGTTGT AGGAGAGTTG 
GTGGGACACA CTGAAAGAGT ATCTGGTTTC ACTTTTTCTC ATCACCCTGG ACAATACAAC 
CTCTGTGCCA CCAGCTCAGA TGATGGGACT GTGAAGATTT GGGATGTAGA GACCAAGGCT 
GTAGTGACAG AACACGCGCT CCATCAGCAT ACAATATCAG CACTGCATTG GTCTCCGACA 
GTTAAGGACT TAATAGTCTC TGGGGATGAA AAAGGAGTTG TTTTCTGTTA CTGGCTTAAC 
AGAAGTGACA GCCAGCACCT CTTCACAGAG CCCAGGGCCA TATTCTGTCT GACCTGTTCA 
CCTCATCACG AAAATCTAGT AGCCATTGGC TACAAAGACG GCATAGTGAT TATCATTGAC 
ATCAGTAAAA AGGGAGAAGT TGTTCACAGG CTTCGAGGGC ACGACGATGA AATCCACTCC 
ATAGCCTGGT GTCCTTTGTC TGGTGAAGAC TGCTTATCTA TAAACCAAGA GGAAAACTCA 
GAACCTGACA TTCCCAATGG GAAGCTTATA GCAGAAACCC CCATCACAAA AGGCTGCTAC 
TTAGCTACAG GAAGCAAGGA TCAGACCATT CGAATCTGGA GCTGTTCCCG AGGCCGGGGT 
GTAATGGTTT TGAAATTGCC CTTTCTGAAG AGAAGAAGTG GGGGTGTGGA CCCAACAGTT 
AAAGAACGTC TTTGGTTGAC ACTTCATTGG CCCAAGAACC AACCAACACA GCTGGTATCC 
AGTTGTTTTG GAGGTGAGCT GCTGCTGTGG GACCTCACCC AGTCATGGAG ACGGAAGTAC 
ACGCTCTTCA GCACCTCAGC GGAAGGGCAC AATCACTCCA GAATTGTCTT CAACTTGTGT 
CCTTTGAAGA CTGAGGATGG CAAACAGCTG CTATTGTCCA CGTCCATGGA CAGAGATGTA 
AAATGTTGGG ACATGGCCAC CCTGGAGTGT TGCTGGACAT TACCTTCTCT TGGAGGTTTT 
GCCTACAGCC TGGCCTTTTC ACCTGTGGAC GTGGGCTCCC TGGCCATAGG TGTTGGGGAC 
GGCATGATCC GGGTATGGAA TACACTCTCC ATAAAGAACA ACTACGATGT GAAAAACTTC 
TGGCAAGGAG TCAAGTCCAA GGTCACGGCG CTATGCTGGC ATCCAAACAA GGAGGGCTGC 
TTAGCCTTTG GGACCGATGA CGGAAAAGTG GGATTGTATG ACACCTGCTC CAACAAACCT 
CCACAGATTT CAAGCACATA TCATAAGAAG ACCGTATATA GGTTGGCTTG GGGGCCACCA 
GTGCCCCCCA TGTCACTTGG AGGGGAAGGA GACAGGCCTT CCCTCACCTT GTACAGCTGT 
GGAGGGGAAG GCATTGTGCT GCAGCATAAT CCCTGGAAGC TCAGTGGAGA GGCCTTTGAC 
ATCAACAAAC TCATGAGAGA CACCAACTCC ATTAGATACA AGCTGCCTGT GCACACAGAG 
ATCAGCTGGA AAGGAGATGG CAAAGTCATG GCTCTTGGAA ATGAAGATGG ATCAATAGAA 
ATATTCCAAG TCCCAAACTT GAAACTGCTC TGCACCATCC AGCAGCACCA CAAGCTTGTG 
AACGCCATAG TCTGGCACCA TGAGCATGGC AGCCGTCCAG AGCTGAGCTG CCTCCTTGCT 
TCTGGCTCCA ACAATGCAGT TATCTACGTG CACAACCTGA AAGCTGTCCT AGAGAGCAAT 
CCTGAGTCTC CAATAACCAT CACAGAGCCC TACCGGACCC TCTCAGGGCA CACAGCCAAG 
GTTACCAGTC TGGCATGGAG CCCACATCAT GATGGAAGGC TGGTATCTGC TTGCTACGAT 
GGCACAGCTC AGGTGTGGGA TGCCCTCCGA GAAGAACCTT TGTTCAATTT CCGAGGACAT 
CGAGGCCGGC TGTTATGTGT TGCGTGGTCC CCAGTGGATC CAGAATGCAT CTACTCAGGT 
GCAGATGACT TCTGTGTCTA CAGGTGGCTG ACCTCCATGC AGGACCATTC CCGGCCTCCT 
CAAGGGAAAA AATGTATTGA ATTAGAGAAA AAACGGCTCT CTCAATTTAA GCCAAAGCTA 
AAAAAGAAGA AAAAACCTAC CTTGCGACTG CCCATAAAGC AAGATTCATC CATTGGCAAT 
GAAGATGAGA GTGTGAGGGA GAACTTGGCA CCTGTTGAGA ATGGCTTGTC CGATCAGGAT 
GGTGAGGAGG AAGCCCAGGA GCCGGAGCTG CCTCCCTCTC CCGTGGTTTG TGGAGAACCT 
GTTTCCTGCA CTGCTGTCTC CTTAGGCTTG GAAAAGTCAA AAGTCACTGT TACTAGTAAA 
GTCGCCTCAC TGAAAAAGGA GCCACCTAAA GAGAAGCCAG AAGCCTTGCT CAAGAAGAGG 
AAGGCTCGCT CCATGCTCCC CCTGAGCACC AGCTTAGACC ACAGGTCCAA AGAGGAGCTC 
CATCGAGACT GTTTGGTTCT AGCAACGGCA ACACACGCCA GAGCAGAACT GCATGAAGAT 
GTGTCTGCCG ACCTTGAGGA ACGATTTCAC TTGGGCCTTT TCACAGACAG GGCTGCTCTG 
TACAGGATGA TGGAGACAGA GGGGAAAGGT CACTTGGAAA GTGGCCACCC CGAGCTTTTT 
CACCAGCTCA TGCTCTGGAA GGGAGATCTG AAAGGCGTTC TCCAGGCTGC AGCAGAGAGA 
GGCGAGCTCA CAGACAGTCT CGTGGCCGTG GCACCAGTAG CTGGCTACAG TGTGTGGCTG 
TGGGCTGTGG AAGCCTTTGC CAAGCAGCTG TGTTTCCAGG ATCAGTATGT CAAGGCTGCC 
TCCTACCTGC TATCCATCCA CAGAGTGTAC GAAGCTGTGG AGCTTCTCAG GTCAAACCAT 
CTCTACAGAG AAGCTATTGC AGTTGCCAAG GCTCGGCTGC GCCCTGAGGA CCCTGTTCTG 
AAGGACCTGT ACCTCAGCTG GGGGTCCATC CTGGAGAGAG ATGGCCACTA TGCAATTGCA 
GCCAAGTGCT ACTTAGGAGC CACTTCCGCC TATGATGCAG CCAAAGTCCT AGCCAGAAAG 
GGAGATGCAG CCTCTCTTAG AACAGCTGCA GAGCTGGCTG CCATAGCAGG AGAGAGTGAG 
CTGGCTGCTT CCCTGGCTCT TAGGTGCGCC CAAGAGCTGC TTCTGCTGAA GAACTGGGTG 
GGTGCACAGG AAGCCTTGGG ACTGCATGAA AGCCTGCAGG GCCAGAGACT GGTGTTCTGC 
CTCCTTGAGC TTCTATGCCG GCACCTGGAG GAAAAACAGC CTCCAGAGGT CAGAGGCCCC 
TCTTCCATTT ACCACCAGTG GGCCACTGGT TCTGAGGGAA CCTTGGTGCA GAGAGTAACT 
GGTGTGTGGC GCAGCGCCTT CAGTGTGGAC ACCCCGGAGC AGTGTCGGGC CGCCTTGCAG 
AAGCTACAGG ATGTCAAGTA CCCATCAGCA ACAAGTAACA CTCCCTTCAG ACAGCTGCTG 
CTTCATGTCT GCCATGACCT AACCTTAGCA ATGCTGAGCC AGCAGGCTGC TTCCTGGGAG 
GAGGCTGTCC CTGCACTGCT GCAGGCTGTG GTTCGGAGCT ACACCTCAGG GAATTTCACC 
CTCATGCAAG AAATCTACTC AGCCTTTCTC CCAGGCGGCT GTGACCACCT ACGAGACAAA 
CTAGGTGACC TATCTCCTAC CATGGCGGCT TTCAAAAGCT TAGAGGCCTT TTTCATTTAT 
GGGCAACTGT ATGAAGTCTG GTGGTCACTC TGTGGGCCTG GCTCAGAATC AAGCATCTGG 
GTGTGGTCGG CTGAAAGCAC AGTCTCTGAT AAACAGAGCA AGCCAGGGGA CAGTGCCAGT 
GCTGAAGACA TGGAGCAGCC TCTAGGCCCA GGCCTGAGGC TCAGTGCGGA GAGTGAACGT 
TTGCTGAGTG CCTGCAAGGA GCTCTTCTCA GAGAGGCATG CCAGCCTACA GACCTCCCAG 
AGGACTGTTG CTGAAGTCCA AGAGACGCTG GCAGAGATGA TCCGCCAGCA CCAAAAGAGT 
CAGCTCTGCA AGGCCACAGC CAACGGCCCC AACAGAGATG AACCCAGCCA GCAAGCAGAG 
CGGGCCCCTT CCCAACCTCC GAGCCCAACC AGAGAAGAAA GAAATGCACC GGTGTCTCTC 
CCTGAATTAA CCAGAAGGCT CACAGAGGCA AACGAGAGAA TAGCTGAGTT TCCAGAGAGT 
GTAAAGGCCT GGCCCTTTCC AGATGTGCTG GAGTGCTGTC TCGTCTTACT TCACATCGGG 
TCCCAGTGTC CTGGTGCTGT GAACCCTGAG ATGCAGCAAC AGGCCCAGAA GCTCCTTCAG 
AAGTATGGCC ACACTAGAGC TTACAGAAGA CACTGCCAGA TTCGACACAC ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006246460.1
CDS	93..4586
Translation	MKPEPRTLPPSPNWYCARCSDAAPGGIFGFAARTSVFLVRVGPGAGASPGAPPFRVVGELVGHTERVSGFTFSHHPGQYNLCATSSDDGTVKIWDVETKAVVTEHALHQHTISALHWSPTVKDLIVSGDEKGVVFCYWLNRSDSQHLFTEPRAIFCLTCSPHHENLVAIGYKDGIVIIIDISKKGEVVHRLRGHDDEIHSIAWCPLSGEDCLSINQEENSEPDIPNGKLIAETPITKGCYLATGSKDQTIRIWSCSRGRGVMVLKLPFLKRRSGGVDPTVKERLWLTLHWPKNQPTQLVSSCFGGELLLWDLTQSWRRKYTLFSTSAEGHNHSRIVFNLCPLKTEDGKQLLLSTSMDRDVKCWDMATLECCWTLPSLGGFAYSLAFSPVDVGSLAIGVGDGMIRVWNTLSIKNNYDVKNFWQGVKSKVTALCWHPNKEGCLAFGTDDGKVGLYDTCSNKPPQISSTYHKKTVYRLAWGPPVPPMSLGGEGDRPSLTLYSCGGEGIVLQHNPWKLSGEAFDINKLMRDTNSIRYKLPVHTEISWKGDGKVMALGNEDGSIEIFQVPNLKLLCTIQQHHKLVNAIVWHHEHGSRPELSCLLASGSNNAVIYVHNLKAVLESNPESPITITEPYRTLSGHTAKVTSLAWSPHHDGRLVSACYDGTAQVWDALREEPLFNFRGHRGRLLCVAWSPVDPECIYSGADDFCVYRWLTSMQDHSRPPQGKKCIELEKKRLSQFKPKLKKKKKPTLRLPIKQDSSIGNEDESVRENLAPVENGLSDQDGEEEAQEPELPPSPVVCGEPVSCTAVSLGLEKSKVTVTSKVASLKKEPPKEKPEALLKKRKARSMLPLSTSLDHRSKEELHRDCLVLATATHARAELHEDVSADLEERFHLGLFTDRAALYRMMETEGKGHLESGHPELFHQLMLWKGDLKGVLQAAAERGELTDSLVAVAPVAGYSVWLWAVEAFAKQLCFQDQYVKAASYLLSIHRVYEAVELLRSNHLYREAIAVAKARLRPEDPVLKDLYLSWGSILERDGHYAIAAKCYLGATSAYDAAKVLARKGDAASLRTAAELAAIAGESELAASLALRCAQELLLLKNWVGAQEALGLHESLQGQRLVFCLLELLCRHLEEKQPPEVRGPSSIYHQWATGSEGTLVQRVTGVWRSAFSVDTPEQCRAALQKLQDVKYPSATSNTPFRQLLLHVCHDLTLAMLSQQAASWEEAVPALLQAVVRSYTSGNFTLMQEIYSAFLPGGCDHLRDKLGDLSPTMAAFKSLEAFFIYGQLYEVWWSLCGPGSESSIWVWSAESTVSDKQSKPGDSASAEDMEQPLGPGLRLSAESERLLSACKELFSERHASLQTSQRTVAEVQETLAEMIRQHQKSQLCKATANGPNRDEPSQQAERAPSQPPSPTREERNAPVSLPELTRRLTEANERIAEFPESVKAWPFPDVLECCLVLLHIGSQCPGAVNPEMQQQAQKLLQKYGHTRAYRRHCQIRHT

Target ORF information:

RefSeq Version	XM_006246398.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus gem (nuclear organelle) associated protein 5 (Gemin5), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006246398.2

ORF Insert Sequence:

ATGAAACCAG AGCCGCGGAC GCTTCCGCCG TCCCCCAACT GGTACTGCGC GCGCTGCAGC 
GACGCGGCGC CCGGAGGCAT CTTTGGCTTC GCTGCGCGGA CCTCCGTCTT CCTCGTCCGC 
GTGGGCCCGG GCGCCGGCGC GAGCCCAGGG GCGCCCCCGT TTCGAGTTGT AGGAGAGTTG 
GTGGGACACA CTGAAAGAGT ATCTGGTTTC ACTTTTTCTC ATCACCCTGG ACAATACAAC 
CTCTGTGCCA CCAGCTCAGA TGATGGGACT GTGAAGATTT GGGATGTAGA GACCAAGGCT 
GTAGTGACAG AACACGCGCT CCATCAGCAT ACAATATCAG CACTGCATTG GTCTCCGACA 
GTTAAGGACT TAATAGTCTC TGGGGATGAA AAAGGAGTTG TTTTCTGTTA CTGGCTTAAC 
AGAAGTGACA GCCAGCACCT CTTCACAGAG CCCAGGGCCA TATTCTGTCT GACCTGTTCA 
CCTCATCACG AAAATCTAGT AGCCATTGGC TACAAAGACG GCATAGTGAT TATCATTGAC 
ATCAGTAAAA AGGGAGAAGT TGTTCACAGG CTTCGAGGGC ACGACGATGA AATCCACTCC 
ATAGCCTGGT GTCCTTTGTC TGGTGAAGAC TGCTTATCTA TAAACCAAGA GGAAAACTCA 
GAACCTGACA TTCCCAATGG GAAGCTTATA GCAGAAACCC CCATCACAAA AGGCTGCTAC 
TTAGCTACAG GAAGCAAGGA TCAGACCATT CGAATCTGGA GCTGTTCCCG AGGCCGGGGT 
GTAATGGTTT TGAAATTGCC CTTTCTGAAG AGAAGAAGTG GGGGTGTGGA CCCAACAGTT 
AAAGAACGTC TTTGGTTGAC ACTTCATTGG CCCAAGAACC AACCAACACA GCTGGTATCC 
AGTTGTTTTG GAGGTGAGCT GCTGCTGTGG GACCTCACCC AGTCATGGAG ACGGAAGTAC 
ACGCTCTTCA GCACCTCAGC GGAAGGGCAC AATCACTCCA GAATTGTCTT CAACTTGTGT 
CCTTTGAAGA CTGAGGATGG CAAACAGCTG CTATTGTCCA CGTCCATGGA CAGAGATGTA 
AAATGTTGGG ACATGGCCAC CCTGGAGTGT TGCTGGACAT TACCTTCTCT TGGAGGTTTT 
GCCTACAGCC TGGCCTTTTC ACCTGTGGAC GTGGGCTCCC TGGCCATAGG TGTTGGGGAC 
GGCATGATCC GGGTATGGAA TACACTCTCC ATAAAGAACA ACTACGATGT GAAAAACTTC 
TGGCAAGGAG TCAAGTCCAA GGTCACGGCG CTATGCTGGC ATCCAAACAA GGAGGGCTGC 
TTAGCCTTTG GGACCGATGA CGGAAAAGTG GGATTGTATG ACACCTGCTC CAACAAACCT 
CCACAGATTT CAAGCACATA TCATAAGAAG ACCGTATATA GGTTGGCTTG GGGGCCACCA 
GTGCCCCCCA TGTCACTTGG AGGGGAAGGA GACAGGCCTT CCCTCACCTT GTACAGCTGT 
GGAGGGGAAG GCATTGTGCT GCAGCATAAT CCCTGGAAGC TCAGTGGAGA GGCCTTTGAC 
ATCAACAAAC TCATGAGAGA CACCAACTCC ATTAGATACA AGCTGCCTGT GCACACAGAG 
ATCAGCTGGA AAGGAGATGG CAAAGTCATG GCTCTTGGAA ATGAAGATGG ATCAATAGAA 
ATATTCCAAG TCCCAAACTT GAAACTGCTC TGCACCATCC AGCAGCACCA CAAGCTTGTG 
AACGCCATAG TCTGGCACCA TGAGCATGGC AGCCGTCCAG AGCTGAGCTG CCTCCTTGCT 
TCTGGCTCCA ACAATGCAGT TATCTACGTG CACAACCTGA AAGCTGTCCT AGAGAGCAAT 
CCTGAGTCTC CAATAACCAT CACAGAGCCC TACCGGACCC TCTCAGGGCA CACAGCCAAG 
GTTACCAGTC TGGCATGGAG CCCACATCAT GATGGAAGGC TGGTATCTGC TTGCTACGAT 
GGCACAGCTC AGGTGTGGGA TGCCCTCCGA GAAGAACCTT TGTTCAATTT CCGAGGACAT 
CGAGGCCGGC TGTTATGTGT TGCGTGGTCC CCAGTGGATC CAGAATGCAT CTACTCAGGT 
GCAGATGACT TCTGTGTCTA CAGGTGGCTG ACCTCCATGC AGGACCATTC CCGGCCTCCT 
CAAGGGAAAA AATGTATTGA ATTAGAGAAA AAACGGCTCT CTCAATTTAA GCCAAAGCTA 
AAAAAGAAGA AAAAACCTAC CTTGCGACTG CCCATAAAGC AAGATTCATC CATTGGCAAT 
GAAGATGAGA GTGTGAGGGA GAACTTGGCA CCTGTTGAGA ATGGCTTGTC CGATCAGGAT 
GGTGAGGAGG AAGCCCAGGA GCCGGAGCTG CCTCCCTCTC CCGTGGTTTG TGGAGAACCT 
GTTTCCTGCA CTGCTGTCTC CTTAGGCTTG GAAAAGTCAA AAGTCACTGT TACTAGTAAA 
GTCGCCTCAC TGAAAAAGGA GCCACCTAAA GAGAAGCCAG AAGCCTTGCT CAAGAAGAGG 
AAGGCTCGCT CCATGCTCCC CCTGAGCACC AGCTTAGACC ACAGGTCCAA AGAGGAGCTC 
CATCGAGACT GTTTGGTTCT AGCAACGGCA ACACACGCCA GAGCAGAACT GCATGAAGAT 
GTGTCTGCCG ACCTTGAGGA ACGATTTCAC TTGGGCCTTT TCACAGACAG GGCTGCTCTG 
TACAGGATGA TGGAGACAGA GGGGAAAGGT CACTTGGAAA GTGGCCACCC CGAGCTTTTT 
CACCAGCTCA TGCTCTGGAA GGGAGATCTG AAAGGCGTTC TCCAGGCTGC AGCAGAGAGA 
GGCGAGCTCA CAGACAGTCT CGTGGCCGTG GCACCAGTAG CTGGCTACAG TGTGTGGCTG 
TGGGCTGTGG AAGCCTTTGC CAAGCAGCTG TGTTTCCAGG ATCAGTATGT CAAGGCTGCC 
TCCTACCTGC TATCCATCCA CAGAGTGTAC GAAGCTGTGG AGCTTCTCAG GTCAAACCAT 
CTCTACAGAG AAGCTATTGC AGTTGCCAAG GCTCGGCTGC GCCCTGAGGA CCCTGTTCTG 
AAGGACCTGT ACCTCAGCTG GGGGTCCATC CTGGAGAGAG ATGGCCACTA TGCAATTGCA 
GCCAAGTGCT ACTTAGGAGC CACTTCCGCC TATGATGCAG CCAAAGTCCT AGCCAGAAAG 
GGAGATGCAG CCTCTCTTAG AACAGCTGCA GAGCTGGCTG CCATAGCAGG AGAGAGTGAG 
CTGGCTGCTT CCCTGGCTCT TAGGTGCGCC CAAGAGCTGC TTCTGCTGAA GAACTGGGTG 
GGTGCACAGG AAGCCTTGGG ACTGCATGAA AGCCTGCAGG GCCAGAGACT GGTGTTCTGC 
CTCCTTGAGC TTCTATGCCG GCACCTGGAG GAAAAACAGC CTCCAGAGGT CAGAGGCCCC 
TCTTCCATTT ACCACCAGTG GGCCACTGGT TCTGAGGGAA CCTTGGTGCA GAGAGTAACT 
GGTGTGTGGC GCAGCGCCTT CAGTGTGGAC ACCCCGGAGC AGTGTCGGGC CGCCTTGCAG 
AAGCTACAGG ATGTCAAGTA CCCATCAGCA ACAAGTAACA CTCCCTTCAG ACAGCTGCTG 
CTTCATGTCT GCCATGACCT AACCTTAGCA ATGCTGAGCC AGCAGGCTGC TTCCTGGGAG 
GAGGCTGTCC CTGCACTGCT GCAGGCTGTG GTTCGGAGCT ACACCTCAGG GAATTTCACC 
CTCATGCAAG AAATCTACTC AGCCTTTCTC CCAGGCGGCT GTGACCACCT ACGAGACAAA 
CTAGGTGACC TATCTCCTAC CATGGCGGCT TTCAAAAGCT TAGAGGCCTT TTTCATTTAT 
GGGCAACTGT ATGAAGTCTG GTGGTCACTC TGTGGGCCTG GCTCAGAATC AAGCATCTGG 
GTGTGGTCGG CTGAAAGCAC AGTCTCTGAT AAACAGAGCA AGCCAGGGGA CAGTGCCAGT 
GCTGAAGACA TGGAGCAGCC TCTAGGCCCA GGCCTGAGGC TCAGTGCGGA GAGTGAACGT 
TTGCTGAGTG CCTGCAAGGA GCTCTTCTCA GAGAGGCATG CCAGCCTACA GACCTCCCAG 
AGGACTGTTG CTGAAGTCCA AGAGACGCTG GCAGAGATGA TCCGCCAGCA CCAAAAGAGT 
CAGCTCTGCA AGGCCACAGC CAACGGCCCC AACAGAGATG AACCCAGCCA GCAAGCAGAG 
CGGGCCCCTT CCCAACCTCC GAGCCCAACC AGAGAAGAAA GAAATGCACC GGTGTCTCTC 
CCTGAATTAA CCAGAAGGCT CACAGAGGCA AACGAGAGAA TAGCTGAGTT TCCAGAGAGT 
GTAAAGGCCT GGCCCTTTCC AGATGTGCTG GAGTGCTGTC TCGTCTTACT TCACATCGGG 
TCCCAGTGTC CTGGTGCTGT GAACCCTGAG ATGCAGCAAC AGGCCCAGAA GCTCCTTCAG 
AAGTATGGCC ACACTAGAGC TTACAGAAGA CACTGCCAGA TTCGACACAC ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gemin5 ( XM_006246399.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006246461.1 Rattus norvegicus gem (nuclear organelle) associated protein 5 (Gemin5), transcript variant X4, mRNA.

Price & Availability↑

CloneID	ORa33120
Clone ID Related Accession (Same CDS sequence)	XM_006246399.2
Accession Version	XM_006246399.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4491bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	gem-associated protein 5 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005109.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 7, 2014 this sequence version replaced XM_006246399.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAACCAG AGCCGCGGAC GCTTCCGCCG TCCCCCAACT GGTACTGCGC GCGCTGCAGC 
GACGCGGCGC CCGGAGGCAT CTTTGGCTTC GCTGCGCGGA CCTCCGTCTT CCTCGTCCGC 
GTGGGCCCGG GCGCCGGCGC GAGCCCAGGG GCGCCCCCGT TTCGAGTTGT AGGAGAGTTG 
GTGGGACACA CTGAAAGAGT ATCTGGTTTC ACTTTTTCTC ATCACCCTGG ACAATACAAC 
CTCTGTGCCA CCAGCTCAGA TGATGGGACT GTGAAGATTT GGGATGTAGA GACCAAGGCT 
GTAGTGACAG AACACGCGCT CCATCAGCAT ACAATATCAG CACTGCATTG GTCTCCGACA 
GTTAAGGACT TAATAGTCTC TGGGGATGAA AAAGGAGTTG TTTTCTGTTA CTGGCTTAAC 
AGAAGTGACA GCCAGCACCT CTTCACAGAG CCCAGGGCCA TATTCTGTCT GACCTGTTCA 
CCTCATCACG AAAATCTAGT AGCCATTGGC TACAAAGACG GCATAGTGAT TATCATTGAC 
ATCAGTAAAA AGGGAGAAGT TGTTCACAGG CTTCGAGGGC ACGACGATGA AATCCACTCC 
ATAGCCTGGT GTCCTTTGTC TGGTGAAGAC TGCTTATCTA TAAACCAAGA GGAAAACTCA 
GAACCTGACA TTCCCAATGG GAAGCTTATA GCAGAAACCC CCATCACAAA AGGCTGCTAC 
TTAGCTACAG GAAGCAAGGA TCAGACCATT CGAATCTGGA GCTGTTCCCG AGGCCGGGGT 
GTAATGGTTT TGAAATTGCC CTTTCTGAAG AGAAGAAGTG GGGGTGTGGA CCCAACAGTT 
AAAGAACGTC TTTGGTTGAC ACTTCATTGG CCCAAGAACC AACCAACACA GCTGGTATCC 
AGTTGTTTTG GAGGTGAGCT GCTGCTGTGG GACCTCACCC AGTCATGGAG ACGGAAGTAC 
ACGCTCTTCA GCACCTCAGC GGAAGGGCAC AATCACTCCA GAATTGTCTT CAACTTGTGT 
CCTTTGAAGA CTGAGGATGG CAAACAGCTG CTATTGTCCA CGTCCATGGA CAGAGATGTA 
AAATGTTGGG ACATGGCCAC CCTGGAGTGT TGCTGGACAT TACCTTCTCT TGGAGGTTTT 
GCCTACAGCC TGGCCTTTTC ACCTGTGGAC GTGGGCTCCC TGGCCATAGG TGTTGGGGAC 
GGCATGATCC GGGTATGGAA TACACTCTCC ATAAAGAACA ACTACGATGT GAAAAACTTC 
TGGCAAGGAG TCAAGTCCAA GGTCACGGCG CTATGCTGGC ATCCAAACAA GGAGGGCTGC 
TTAGCCTTTG GGACCGATGA CGGAAAAGTG GGATTGTATG ACACCTGCTC CAACAAACCT 
CCACAGATTT CAAGCACATA TCATAAGAAG ACCGTATATA GGTTGGCTTG GGGGCCACCA 
GTGCCCCCCA TGTCACTTGG AGGGGAAGGA GACAGGCCTT CCCTCACCTT GTACAGCTGT 
GGAGGGGAAG GCATTGTGCT GCAGCATAAT CCCTGGAAGC TCAGTGGAGA GGCCTTTGAC 
ATCAACAAAC TCATGAGAGA CACCAACTCC ATTAGATACA AGCTGCCTGT GCACACAGAG 
ATCAGCTGGA AAGGAGATGG CAAAGTCATG GCTCTTGGAA ATGAAGATGG ATCAATAGAA 
ATATTCCAAG TCCCAAACTT GAAACTGCTC TGCACCATCC AGCAGCACCA CAAGCTTGTG 
AACGCCATAG TCTGGCACCA TGAGCATGGC AGCCGTCCAG AGCTGAGCTG CCTCCTTGCT 
TCTGGCTCCA ACAATGCAGT TATCTACGTG CACAACCTGA AAGCTGTCCT AGAGAGCAAT 
CCTGAGTCTC CAATAACCAT CACAGAGCCC TACCGGACCC TCTCAGGGCA CACAGCCAAG 
GTTACCAGTC TGGCATGGAG CCCACATCAT GATGGAAGGC TGGTATCTGC TTGCTACGAT 
GGCACAGCTC AGGTGTGGGA TGCCCTCCGA GAAGAACCTT TGTTCAATTT CCGAGGACAT 
CGAGGCCGGC TGTTATGTGT TGCGTGGTCC CCAGTGGATC CAGAATGCAT CTACTCAGGT 
GCAGATGACT TCTGTGTCTA CAGGTGGCTG ACCTCCATGC AGGACCATTC CCGGCCTCCT 
CAAGGGAAAA AATGTATTGA ATTAGAGAAA AAACGGCTCT CTCAATTTAA GCCAAAGCTA 
AAAAAGAAGA AAAAACCTAC CTTGCGACTG CCCATAAAGC AAGATTCATC CATTGGCAAT 
GAAGATGAGA GTGTGAGGGA GAACTTGGCA CCTGTTGAGA ATGGCTTGTC CGATCAGGAT 
GGTGAGGAGG AAGCCCAGGA GCCGGAGCTG CCTCCCTCTC CCGTGGTTTG TGGAGAACCT 
GTTTCCTGCA CTGCTGTCTC CTTAGGCTTG GAAAAGTCAA AAGTCACTGT TACTAGTAAA 
GTCGCCTCAC TGAAAAAGGA GCCACCTAAA GAGAAGCCAG AAGCCTTGCT CAAGAAGAGG 
AAGGCTCGCT CCATGCTCCC CCTGAGCACC AGCTTAGACC ACAGGTCCAA AGAGGAGCTC 
CATCGAGACT GTTTGGTTCT AGCAACGGCA ACACACGCCA GAGCAGAACT GCATGAAGAT 
GTGTCTGCCG ACCTTGAGGA ACGATTTCAC TTGGGCCTTT TCACAGACAG GGCTGCTCTG 
TACAGGATGA TGGAGACAGA GGGGAAAGGT CACTTGGAAA GTGGCCACCC CGAGCTTTTT 
CACCAGCTCA TGCTCTGGAA GGGAGATCTG AAAGGCGTTC TCCAGGCTGC AGCAGAGAGA 
GGCGAGCTCA CAGACAGTCT CGTGGCCGTG GCACCAGTAG CTGGCTACAG TGTGTGGCTG 
TGGGCTGTGG AAGCCTTTGC CAAGCAGCTG TGTTTCCAGG ATCAGTATGT CAAGGCTGCC 
TCCTACCTGC TATCCATCCA CAGAGTGTAC GAAGCTGTGG AGCTTCTCAG GTCAAACCAT 
CTCTACAGAG AAGCTATTGC AGTTGCCAAG GCTCGGCTGC GCCCTGAGGA CCCTGTTCTG 
AAGGACCTGT ACCTCAGCTG GGGGTCCATC CTGGAGAGAG ATGGCCACTA TGCAATTGCA 
GCCAAGTGCT ACTTAGGAGC CACTTCCGCC TATGATGCAG CCAAAGTCCT AGCCAGAAAG 
GGAGATGCAG CCTCTCTTAG AACAGCTGCA GAGCTGGCTG CCATAGCAGG AGAGAGTGAG 
CTGGCTGCTT CCCTGGCTCT TAGGTGCGCC CAAGAGCTGC TTCTGCTGAA GAACTGGGTG 
GGTGCACAGG AAGCCTTGGG ACTGCATGAA AGCCTGCAGG GCCAGAGACT GGTGTTCTGC 
CTCCTTGAGC TTCTATGCCG GCACCTGGAG GAAAAACAGC CTCCAGAGGT CAGAGGCCCC 
TCTTCCATTT ACCACCAGTG GGCCACTGGT TCTGAGGGAA CCTTGGTGCA GAGAGTAACT 
GGTGTGTGGC GCAGCGCCTT CAGTGTGGAC ACCCCGGAGC AGTGTCGGGC CGCCTTGCAG 
AAGCTACAGG ATGTCAAGTA CCCATCAGCA ACAAGTAACA CTCCCTTCAG ACAGCTGCTG 
CTTCATGTCT GCCATGACCT AACCTTAGCA ATGCTGAGCC AGCAGGCTGC TTCCTGGGAG 
GAGGCTGTCC CTGCACTGCT GCAGGCTGTG GTTCGGAGCT ACACCTCAGG GAATTTCACC 
CTCATGCAAG AAATCTACTC AGCCTTTCTC CCAGGCGGCT GTGACCACCT ACGAGACAAA 
CTAGGTGACC TATCTCCTAC CATGGCGGCT TTCAAAAGCT TAGAGGCCTT TTTCATTTAT 
GGGCAACTGT ATGAAGTCTG GTGGTCACTC TGTGGGCCTG GCTCAGAATC AAGCATCTGG 
GTGTGGTCGG CTGAAAGCAC AGTCTCTGAT AAACAGAGCA AGCCAGGGGA CAGTGCCAGT 
GCTGAAGACA TGGAGCAGCC TCTAGGCCCA GGCCTGAGGC TCAGTGCGGA GAGTGAACGT 
TTGCTGAGTG CCTGCAAGGA GCTCTTCTCA GAGAGGCATG CCAGCCTACA GACCTCCCAG 
AGGACTGTTG CTGAAGTCCA AGAGACGCTG GCAGAGATGA TCCGCCAGCA CCAAAAGAGT 
CAGCTCTGCA AGGCCACAGC CAACGGCCCC AACAGAGATG AACCCAGCCA GCAAGCAGAG 
CGGGCCCCTT CCCAACCTCC GAGCCCAACA GAAGAAAGAA ATGCACCGGT GTCTCTCCCT 
GAATTAACCA GAAGGCTCAC AGAGGCAAAC GAGAGAATAG CTGAGTTTCC AGAGAGTGTA 
AAGGCCTGGC CCTTTCCAGA TGTGCTGGAG TGCTGTCTCG TCTTACTTCA CATCGGGTCC 
CAGTGTCCTG GTGCTGTGAA CCCTGAGATG CAGCAACAGG CCCAGAAGCT CCTTCAGAAG 
TATGGCCACA CTAGAGCTTA CAGAAGACAC TGCCAGATTC GACACACATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006246461.1
CDS	93..4583
Translation	MKPEPRTLPPSPNWYCARCSDAAPGGIFGFAARTSVFLVRVGPGAGASPGAPPFRVVGELVGHTERVSGFTFSHHPGQYNLCATSSDDGTVKIWDVETKAVVTEHALHQHTISALHWSPTVKDLIVSGDEKGVVFCYWLNRSDSQHLFTEPRAIFCLTCSPHHENLVAIGYKDGIVIIIDISKKGEVVHRLRGHDDEIHSIAWCPLSGEDCLSINQEENSEPDIPNGKLIAETPITKGCYLATGSKDQTIRIWSCSRGRGVMVLKLPFLKRRSGGVDPTVKERLWLTLHWPKNQPTQLVSSCFGGELLLWDLTQSWRRKYTLFSTSAEGHNHSRIVFNLCPLKTEDGKQLLLSTSMDRDVKCWDMATLECCWTLPSLGGFAYSLAFSPVDVGSLAIGVGDGMIRVWNTLSIKNNYDVKNFWQGVKSKVTALCWHPNKEGCLAFGTDDGKVGLYDTCSNKPPQISSTYHKKTVYRLAWGPPVPPMSLGGEGDRPSLTLYSCGGEGIVLQHNPWKLSGEAFDINKLMRDTNSIRYKLPVHTEISWKGDGKVMALGNEDGSIEIFQVPNLKLLCTIQQHHKLVNAIVWHHEHGSRPELSCLLASGSNNAVIYVHNLKAVLESNPESPITITEPYRTLSGHTAKVTSLAWSPHHDGRLVSACYDGTAQVWDALREEPLFNFRGHRGRLLCVAWSPVDPECIYSGADDFCVYRWLTSMQDHSRPPQGKKCIELEKKRLSQFKPKLKKKKKPTLRLPIKQDSSIGNEDESVRENLAPVENGLSDQDGEEEAQEPELPPSPVVCGEPVSCTAVSLGLEKSKVTVTSKVASLKKEPPKEKPEALLKKRKARSMLPLSTSLDHRSKEELHRDCLVLATATHARAELHEDVSADLEERFHLGLFTDRAALYRMMETEGKGHLESGHPELFHQLMLWKGDLKGVLQAAAERGELTDSLVAVAPVAGYSVWLWAVEAFAKQLCFQDQYVKAASYLLSIHRVYEAVELLRSNHLYREAIAVAKARLRPEDPVLKDLYLSWGSILERDGHYAIAAKCYLGATSAYDAAKVLARKGDAASLRTAAELAAIAGESELAASLALRCAQELLLLKNWVGAQEALGLHESLQGQRLVFCLLELLCRHLEEKQPPEVRGPSSIYHQWATGSEGTLVQRVTGVWRSAFSVDTPEQCRAALQKLQDVKYPSATSNTPFRQLLLHVCHDLTLAMLSQQAASWEEAVPALLQAVVRSYTSGNFTLMQEIYSAFLPGGCDHLRDKLGDLSPTMAAFKSLEAFFIYGQLYEVWWSLCGPGSESSIWVWSAESTVSDKQSKPGDSASAEDMEQPLGPGLRLSAESERLLSACKELFSERHASLQTSQRTVAEVQETLAEMIRQHQKSQLCKATANGPNRDEPSQQAERAPSQPPSPTEERNAPVSLPELTRRLTEANERIAEFPESVKAWPFPDVLECCLVLLHIGSQCPGAVNPEMQQQAQKLLQKYGHTRAYRRHCQIRHT

Target ORF information:

RefSeq Version	XM_006246399.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus gem (nuclear organelle) associated protein 5 (Gemin5), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006246399.2

ORF Insert Sequence:

ATGAAACCAG AGCCGCGGAC GCTTCCGCCG TCCCCCAACT GGTACTGCGC GCGCTGCAGC 
GACGCGGCGC CCGGAGGCAT CTTTGGCTTC GCTGCGCGGA CCTCCGTCTT CCTCGTCCGC 
GTGGGCCCGG GCGCCGGCGC GAGCCCAGGG GCGCCCCCGT TTCGAGTTGT AGGAGAGTTG 
GTGGGACACA CTGAAAGAGT ATCTGGTTTC ACTTTTTCTC ATCACCCTGG ACAATACAAC 
CTCTGTGCCA CCAGCTCAGA TGATGGGACT GTGAAGATTT GGGATGTAGA GACCAAGGCT 
GTAGTGACAG AACACGCGCT CCATCAGCAT ACAATATCAG CACTGCATTG GTCTCCGACA 
GTTAAGGACT TAATAGTCTC TGGGGATGAA AAAGGAGTTG TTTTCTGTTA CTGGCTTAAC 
AGAAGTGACA GCCAGCACCT CTTCACAGAG CCCAGGGCCA TATTCTGTCT GACCTGTTCA 
CCTCATCACG AAAATCTAGT AGCCATTGGC TACAAAGACG GCATAGTGAT TATCATTGAC 
ATCAGTAAAA AGGGAGAAGT TGTTCACAGG CTTCGAGGGC ACGACGATGA AATCCACTCC 
ATAGCCTGGT GTCCTTTGTC TGGTGAAGAC TGCTTATCTA TAAACCAAGA GGAAAACTCA 
GAACCTGACA TTCCCAATGG GAAGCTTATA GCAGAAACCC CCATCACAAA AGGCTGCTAC 
TTAGCTACAG GAAGCAAGGA TCAGACCATT CGAATCTGGA GCTGTTCCCG AGGCCGGGGT 
GTAATGGTTT TGAAATTGCC CTTTCTGAAG AGAAGAAGTG GGGGTGTGGA CCCAACAGTT 
AAAGAACGTC TTTGGTTGAC ACTTCATTGG CCCAAGAACC AACCAACACA GCTGGTATCC 
AGTTGTTTTG GAGGTGAGCT GCTGCTGTGG GACCTCACCC AGTCATGGAG ACGGAAGTAC 
ACGCTCTTCA GCACCTCAGC GGAAGGGCAC AATCACTCCA GAATTGTCTT CAACTTGTGT 
CCTTTGAAGA CTGAGGATGG CAAACAGCTG CTATTGTCCA CGTCCATGGA CAGAGATGTA 
AAATGTTGGG ACATGGCCAC CCTGGAGTGT TGCTGGACAT TACCTTCTCT TGGAGGTTTT 
GCCTACAGCC TGGCCTTTTC ACCTGTGGAC GTGGGCTCCC TGGCCATAGG TGTTGGGGAC 
GGCATGATCC GGGTATGGAA TACACTCTCC ATAAAGAACA ACTACGATGT GAAAAACTTC 
TGGCAAGGAG TCAAGTCCAA GGTCACGGCG CTATGCTGGC ATCCAAACAA GGAGGGCTGC 
TTAGCCTTTG GGACCGATGA CGGAAAAGTG GGATTGTATG ACACCTGCTC CAACAAACCT 
CCACAGATTT CAAGCACATA TCATAAGAAG ACCGTATATA GGTTGGCTTG GGGGCCACCA 
GTGCCCCCCA TGTCACTTGG AGGGGAAGGA GACAGGCCTT CCCTCACCTT GTACAGCTGT 
GGAGGGGAAG GCATTGTGCT GCAGCATAAT CCCTGGAAGC TCAGTGGAGA GGCCTTTGAC 
ATCAACAAAC TCATGAGAGA CACCAACTCC ATTAGATACA AGCTGCCTGT GCACACAGAG 
ATCAGCTGGA AAGGAGATGG CAAAGTCATG GCTCTTGGAA ATGAAGATGG ATCAATAGAA 
ATATTCCAAG TCCCAAACTT GAAACTGCTC TGCACCATCC AGCAGCACCA CAAGCTTGTG 
AACGCCATAG TCTGGCACCA TGAGCATGGC AGCCGTCCAG AGCTGAGCTG CCTCCTTGCT 
TCTGGCTCCA ACAATGCAGT TATCTACGTG CACAACCTGA AAGCTGTCCT AGAGAGCAAT 
CCTGAGTCTC CAATAACCAT CACAGAGCCC TACCGGACCC TCTCAGGGCA CACAGCCAAG 
GTTACCAGTC TGGCATGGAG CCCACATCAT GATGGAAGGC TGGTATCTGC TTGCTACGAT 
GGCACAGCTC AGGTGTGGGA TGCCCTCCGA GAAGAACCTT TGTTCAATTT CCGAGGACAT 
CGAGGCCGGC TGTTATGTGT TGCGTGGTCC CCAGTGGATC CAGAATGCAT CTACTCAGGT 
GCAGATGACT TCTGTGTCTA CAGGTGGCTG ACCTCCATGC AGGACCATTC CCGGCCTCCT 
CAAGGGAAAA AATGTATTGA ATTAGAGAAA AAACGGCTCT CTCAATTTAA GCCAAAGCTA 
AAAAAGAAGA AAAAACCTAC CTTGCGACTG CCCATAAAGC AAGATTCATC CATTGGCAAT 
GAAGATGAGA GTGTGAGGGA GAACTTGGCA CCTGTTGAGA ATGGCTTGTC CGATCAGGAT 
GGTGAGGAGG AAGCCCAGGA GCCGGAGCTG CCTCCCTCTC CCGTGGTTTG TGGAGAACCT 
GTTTCCTGCA CTGCTGTCTC CTTAGGCTTG GAAAAGTCAA AAGTCACTGT TACTAGTAAA 
GTCGCCTCAC TGAAAAAGGA GCCACCTAAA GAGAAGCCAG AAGCCTTGCT CAAGAAGAGG 
AAGGCTCGCT CCATGCTCCC CCTGAGCACC AGCTTAGACC ACAGGTCCAA AGAGGAGCTC 
CATCGAGACT GTTTGGTTCT AGCAACGGCA ACACACGCCA GAGCAGAACT GCATGAAGAT 
GTGTCTGCCG ACCTTGAGGA ACGATTTCAC TTGGGCCTTT TCACAGACAG GGCTGCTCTG 
TACAGGATGA TGGAGACAGA GGGGAAAGGT CACTTGGAAA GTGGCCACCC CGAGCTTTTT 
CACCAGCTCA TGCTCTGGAA GGGAGATCTG AAAGGCGTTC TCCAGGCTGC AGCAGAGAGA 
GGCGAGCTCA CAGACAGTCT CGTGGCCGTG GCACCAGTAG CTGGCTACAG TGTGTGGCTG 
TGGGCTGTGG AAGCCTTTGC CAAGCAGCTG TGTTTCCAGG ATCAGTATGT CAAGGCTGCC 
TCCTACCTGC TATCCATCCA CAGAGTGTAC GAAGCTGTGG AGCTTCTCAG GTCAAACCAT 
CTCTACAGAG AAGCTATTGC AGTTGCCAAG GCTCGGCTGC GCCCTGAGGA CCCTGTTCTG 
AAGGACCTGT ACCTCAGCTG GGGGTCCATC CTGGAGAGAG ATGGCCACTA TGCAATTGCA 
GCCAAGTGCT ACTTAGGAGC CACTTCCGCC TATGATGCAG CCAAAGTCCT AGCCAGAAAG 
GGAGATGCAG CCTCTCTTAG AACAGCTGCA GAGCTGGCTG CCATAGCAGG AGAGAGTGAG 
CTGGCTGCTT CCCTGGCTCT TAGGTGCGCC CAAGAGCTGC TTCTGCTGAA GAACTGGGTG 
GGTGCACAGG AAGCCTTGGG ACTGCATGAA AGCCTGCAGG GCCAGAGACT GGTGTTCTGC 
CTCCTTGAGC TTCTATGCCG GCACCTGGAG GAAAAACAGC CTCCAGAGGT CAGAGGCCCC 
TCTTCCATTT ACCACCAGTG GGCCACTGGT TCTGAGGGAA CCTTGGTGCA GAGAGTAACT 
GGTGTGTGGC GCAGCGCCTT CAGTGTGGAC ACCCCGGAGC AGTGTCGGGC CGCCTTGCAG 
AAGCTACAGG ATGTCAAGTA CCCATCAGCA ACAAGTAACA CTCCCTTCAG ACAGCTGCTG 
CTTCATGTCT GCCATGACCT AACCTTAGCA ATGCTGAGCC AGCAGGCTGC TTCCTGGGAG 
GAGGCTGTCC CTGCACTGCT GCAGGCTGTG GTTCGGAGCT ACACCTCAGG GAATTTCACC 
CTCATGCAAG AAATCTACTC AGCCTTTCTC CCAGGCGGCT GTGACCACCT ACGAGACAAA 
CTAGGTGACC TATCTCCTAC CATGGCGGCT TTCAAAAGCT TAGAGGCCTT TTTCATTTAT 
GGGCAACTGT ATGAAGTCTG GTGGTCACTC TGTGGGCCTG GCTCAGAATC AAGCATCTGG 
GTGTGGTCGG CTGAAAGCAC AGTCTCTGAT AAACAGAGCA AGCCAGGGGA CAGTGCCAGT 
GCTGAAGACA TGGAGCAGCC TCTAGGCCCA GGCCTGAGGC TCAGTGCGGA GAGTGAACGT 
TTGCTGAGTG CCTGCAAGGA GCTCTTCTCA GAGAGGCATG CCAGCCTACA GACCTCCCAG 
AGGACTGTTG CTGAAGTCCA AGAGACGCTG GCAGAGATGA TCCGCCAGCA CCAAAAGAGT 
CAGCTCTGCA AGGCCACAGC CAACGGCCCC AACAGAGATG AACCCAGCCA GCAAGCAGAG 
CGGGCCCCTT CCCAACCTCC GAGCCCAACA GAAGAAAGAA ATGCACCGGT GTCTCTCCCT 
GAATTAACCA GAAGGCTCAC AGAGGCAAAC GAGAGAATAG CTGAGTTTCC AGAGAGTGTA 
AAGGCCTGGC CCTTTCCAGA TGTGCTGGAG TGCTGTCTCG TCTTACTTCA CATCGGGTCC 
CAGTGTCCTG GTGCTGTGAA CCCTGAGATG CAGCAACAGG CCCAGAAGCT CCTTCAGAAG 
TATGGCCACA CTAGAGCTTA CAGAAGACAC TGCCAGATTC GACACACATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gemin5 ( XM_006246400.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006246462.1 Rattus norvegicus gem (nuclear organelle) associated protein 5 (Gemin5), transcript variant X5, mRNA.

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CloneID	ORa33121
Clone ID Related Accession (Same CDS sequence)	XM_006246400.2
Accession Version	XM_006246400.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4491bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	gem-associated protein 5 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005109.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 7, 2014 this sequence version replaced XM_006246400.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAACCAG AGCCGCGGAC GCTTCCGCCG TCCCCCAACT GGTACTGCGC GCGCTGCAGC 
GACGCGGCGC CCGGAGGCAT CTTTGGCTTC GCTGCGCGGA CCTCCGTCTT CCTCGTCCGC 
GTGGGCCCGG GCGCCGGCGC GAGCCCAGGG GCGCCCCCGT TTCGAGTTGT AGGAGAGTTG 
GTGGGACACA CTGAAAGAGT ATCTGGTTTC ACTTTTTCTC ATCACCCTGG ACAATACAAC 
CTCTGTGCCA CCAGCTCAGA TGATGGGACT GTGAAGATTT GGGATGTAGA GACCAAGGCT 
GTAGTGACAG AACACGCGCT CCATCAGCAT ACAATATCAG CACTGCATTG GTCTCCGACA 
GTTAAGGACT TAATAGTCTC TGGGGATGAA AAAGGAGTTG TTTTCTGTTA CTGGCTTAAC 
AGAAGTGACA GCCAGCACCT CTTCACAGAG CCCAGGGCCA TATTCTGTCT GACCTGTTCA 
CCTCATCACG AAAATCTAGT AGCCATTGGC TACAAAGACG GCATAGTGAT TATCATTGAC 
ATCAGTAAAA AGGGAGAAGT TGTTCACAGG CTTCGAGGGC ACGACGATGA AATCCACTCC 
ATAGCCTGGT GTCCTTTGTC TGGTGAAGAC TGCTTATCTA TAAACCAAGA GGAAAACTCA 
GAACCTGACA TTCCCAATGG GAAGCTTATA GCAGAAACCC CCATCACAAA AGGCTGCTAC 
TTAGCTACAG GAAGCAAGGA TCAGACCATT CGAATCTGGA GCTGTTCCCG AGGCCGGGGT 
GTAATGGTTT TGAAATTGCC CTTTCTGAAG AGAAGAAGTG GGGGTGTGGA CCCAACAGTT 
AAAGAACGTC TTTGGTTGAC ACTTCATTGG CCCAAGAACC AACCAACACA GCTGGTATCC 
AGTTGTTTTG GAGGTGAGCT GCTGCTGTGG GACCTCACCC AGTCATGGAG ACGGAAGTAC 
ACGCTCTTCA GCACCTCAGC GGAAGGGCAC AATCACTCCA GAATTGTCTT CAACTTGTGT 
CCTTTGAAGA CTGAGGATGG CAAACAGCTG CTATTGTCCA CGTCCATGGA CAGAGATGTA 
AAATGTTGGG ACATGGCCAC CCTGGAGTGT TGCTGGACAT TACCTTCTCT TGGAGGTTTT 
GCCTACAGCC TGGCCTTTTC ACCTGTGGAC GTGGGCTCCC TGGCCATAGG TGTTGGGGAC 
GGCATGATCC GGGTATGGAA TACACTCTCC ATAAAGAACA ACTACGATGT GAAAAACTTC 
TGGCAAGGAG TCAAGTCCAA GGTCACGGCG CTATGCTGGC ATCCAAACAA GGAGGGCTGC 
TTAGCCTTTG GGACCGATGA CGGAAAAGTG GGATTGTATG ACACCTGCTC CAACAAACCT 
CCACAGATTT CAAGCACATA TCATAAGAAG ACCGTATATA GGTTGGCTTG GGGGCCACCA 
GTGCCCCCCA TGTCACTTGG AGGGGAAGGA GACAGGCCTT CCCTCACCTT GTACAGCTGT 
GGAGGGGAAG GCATTGTGCT GCAGCATAAT CCCTGGAAGC TCAGTGGAGA GGCCTTTGAC 
ATCAACAAAC TCATGAGAGA CACCAACTCC ATTAGATACA AGCTGCCTGT GCACACAGAG 
ATCAGCTGGA AAGGAGATGG CAAAGTCATG GCTCTTGGAA ATGAAGATGG ATCAATAGAA 
ATATTCCAAG TCCCAAACTT GAAACTGCTC TGCACCATCC AGCAGCACCA CAAGCTTGTG 
AACGCCATAG TCTGGCACCA TGAGCATGGC AGCCGTCCAG AGCTGAGCTG CCTCCTTGCT 
TCTGGCTCCA ACAATGCAGT TATCTACGTG CACAACCTGA AAGCTGTCCT AGAGAGCAAT 
CCTGAGTCTC CAATAACCAT CACAGAGCCC TACCGGACCC TCTCAGGGCA CACAGCCAAG 
GTTACCAGTC TGGCATGGAG CCCACATCAT GATGGAAGGC TGGTATCTGC TTGCTACGAT 
GGCACAGCTC AGGTGTGGGA TGCCCTCCGA GAAGAACCTT TGTTCAATTT CCGAGGACAT 
CGAGGCCGGC TGTTATGTGT TGCGTGGTCC CCAGTGGATC CAGAATGCAT CTACTCAGGT 
GCAGATGACT TCTGTGTCTA CAGGTGGCTG ACCTCCATGC AGGACCATTC CCGGCCTCCT 
CAAGGGAAAA AATGTATTGA ATTAGAGAAA AAACGGCTCT CTCAATTTAA GCCAAAGCTA 
AAAAAGAAGA AAAAACCTAC CTTGCGACTG CCCATAAAGC AAGATTCATC CATTGGCAAT 
GAAGATGAGA GTGTGAGGGA GAACTTGGCA CCTGTTGAGA ATGGCTTGTC CGATCAGGAT 
GGTGAGGAGG AAGCCCAGGA GCCGGAGCTG CCTCCCTCTC CCGTGGTTTG TGGAGAACCT 
GTTTCCTGCA CTGCTGTCTC CTTAGGCTTG GAAAAGTCAA AAGTCACTGT TACTAGTAAA 
GTCGCCTCAC TGAAAAAGGA GCCACCTAAA GAGAAGCCAG AAGCCTTGCT CAAGAAGAGG 
AAGGCTCGCT CCATGCTCCC CCTGAGCACC AGCTTAGACC ACAGGTCCAA AGAGGAGCTC 
CATCGAGACT GTTTGGTTCT AGCAACGGCA ACACACGCCA GAGAACTGCA TGAAGATGTG 
TCTGCCGACC TTGAGGAACG ATTTCACTTG GGCCTTTTCA CAGACAGGGC TGCTCTGTAC 
AGGATGATGG AGACAGAGGG GAAAGGTCAC TTGGAAAGTG GCCACCCCGA GCTTTTTCAC 
CAGCTCATGC TCTGGAAGGG AGATCTGAAA GGCGTTCTCC AGGCTGCAGC AGAGAGAGGC 
GAGCTCACAG ACAGTCTCGT GGCCGTGGCA CCAGTAGCTG GCTACAGTGT GTGGCTGTGG 
GCTGTGGAAG CCTTTGCCAA GCAGCTGTGT TTCCAGGATC AGTATGTCAA GGCTGCCTCC 
TACCTGCTAT CCATCCACAG AGTGTACGAA GCTGTGGAGC TTCTCAGGTC AAACCATCTC 
TACAGAGAAG CTATTGCAGT TGCCAAGGCT CGGCTGCGCC CTGAGGACCC TGTTCTGAAG 
GACCTGTACC TCAGCTGGGG GTCCATCCTG GAGAGAGATG GCCACTATGC AATTGCAGCC 
AAGTGCTACT TAGGAGCCAC TTCCGCCTAT GATGCAGCCA AAGTCCTAGC CAGAAAGGGA 
GATGCAGCCT CTCTTAGAAC AGCTGCAGAG CTGGCTGCCA TAGCAGGAGA GAGTGAGCTG 
GCTGCTTCCC TGGCTCTTAG GTGCGCCCAA GAGCTGCTTC TGCTGAAGAA CTGGGTGGGT 
GCACAGGAAG CCTTGGGACT GCATGAAAGC CTGCAGGGCC AGAGACTGGT GTTCTGCCTC 
CTTGAGCTTC TATGCCGGCA CCTGGAGGAA AAACAGCCTC CAGAGGTCAG AGGCCCCTCT 
TCCATTTACC ACCAGTGGGC CACTGGTTCT GAGGGAACCT TGGTGCAGAG AGTAACTGGT 
GTGTGGCGCA GCGCCTTCAG TGTGGACACC CCGGAGCAGT GTCGGGCCGC CTTGCAGAAG 
CTACAGGATG TCAAGTACCC ATCAGCAACA AGTAACACTC CCTTCAGACA GCTGCTGCTT 
CATGTCTGCC ATGACCTAAC CTTAGCAATG CTGAGCCAGC AGGCTGCTTC CTGGGAGGAG 
GCTGTCCCTG CACTGCTGCA GGCTGTGGTT CGGAGCTACA CCTCAGGGAA TTTCACCCTC 
ATGCAAGAAA TCTACTCAGC CTTTCTCCCA GGCGGCTGTG ACCACCTACG AGACAAACTA 
GGTGACCTAT CTCCTACCAT GGCGGCTTTC AAAAGCTTAG AGGCCTTTTT CATTTATGGG 
CAACTGTATG AAGTCTGGTG GTCACTCTGT GGGCCTGGCT CAGAATCAAG CATCTGGGTG 
TGGTCGGCTG AAAGCACAGT CTCTGATAAA CAGAGCAAGC CAGGGGACAG TGCCAGTGCT 
GAAGACATGG AGCAGCCTCT AGGCCCAGGC CTGAGGCTCA GTGCGGAGAG TGAACGTTTG 
CTGAGTGCCT GCAAGGAGCT CTTCTCAGAG AGGCATGCCA GCCTACAGAC CTCCCAGAGG 
ACTGTTGCTG AAGTCCAAGA GACGCTGGCA GAGATGATCC GCCAGCACCA AAAGAGTCAG 
CTCTGCAAGG CCACAGCCAA CGGCCCCAAC AGAGATGAAC CCAGCCAGCA AGCAGAGCGG 
GCCCCTTCCC AACCTCCGAG CCCAACCAGA GAAGAAAGAA ATGCACCGGT GTCTCTCCCT 
GAATTAACCA GAAGGCTCAC AGAGGCAAAC GAGAGAATAG CTGAGTTTCC AGAGAGTGTA 
AAGGCCTGGC CCTTTCCAGA TGTGCTGGAG TGCTGTCTCG TCTTACTTCA CATCGGGTCC 
CAGTGTCCTG GTGCTGTGAA CCCTGAGATG CAGCAACAGG CCCAGAAGCT CCTTCAGAAG 
TATGGCCACA CTAGAGCTTA CAGAAGACAC TGCCAGATTC GACACACATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006246462.1
CDS	93..4583
Translation	MKPEPRTLPPSPNWYCARCSDAAPGGIFGFAARTSVFLVRVGPGAGASPGAPPFRVVGELVGHTERVSGFTFSHHPGQYNLCATSSDDGTVKIWDVETKAVVTEHALHQHTISALHWSPTVKDLIVSGDEKGVVFCYWLNRSDSQHLFTEPRAIFCLTCSPHHENLVAIGYKDGIVIIIDISKKGEVVHRLRGHDDEIHSIAWCPLSGEDCLSINQEENSEPDIPNGKLIAETPITKGCYLATGSKDQTIRIWSCSRGRGVMVLKLPFLKRRSGGVDPTVKERLWLTLHWPKNQPTQLVSSCFGGELLLWDLTQSWRRKYTLFSTSAEGHNHSRIVFNLCPLKTEDGKQLLLSTSMDRDVKCWDMATLECCWTLPSLGGFAYSLAFSPVDVGSLAIGVGDGMIRVWNTLSIKNNYDVKNFWQGVKSKVTALCWHPNKEGCLAFGTDDGKVGLYDTCSNKPPQISSTYHKKTVYRLAWGPPVPPMSLGGEGDRPSLTLYSCGGEGIVLQHNPWKLSGEAFDINKLMRDTNSIRYKLPVHTEISWKGDGKVMALGNEDGSIEIFQVPNLKLLCTIQQHHKLVNAIVWHHEHGSRPELSCLLASGSNNAVIYVHNLKAVLESNPESPITITEPYRTLSGHTAKVTSLAWSPHHDGRLVSACYDGTAQVWDALREEPLFNFRGHRGRLLCVAWSPVDPECIYSGADDFCVYRWLTSMQDHSRPPQGKKCIELEKKRLSQFKPKLKKKKKPTLRLPIKQDSSIGNEDESVRENLAPVENGLSDQDGEEEAQEPELPPSPVVCGEPVSCTAVSLGLEKSKVTVTSKVASLKKEPPKEKPEALLKKRKARSMLPLSTSLDHRSKEELHRDCLVLATATHARELHEDVSADLEERFHLGLFTDRAALYRMMETEGKGHLESGHPELFHQLMLWKGDLKGVLQAAAERGELTDSLVAVAPVAGYSVWLWAVEAFAKQLCFQDQYVKAASYLLSIHRVYEAVELLRSNHLYREAIAVAKARLRPEDPVLKDLYLSWGSILERDGHYAIAAKCYLGATSAYDAAKVLARKGDAASLRTAAELAAIAGESELAASLALRCAQELLLLKNWVGAQEALGLHESLQGQRLVFCLLELLCRHLEEKQPPEVRGPSSIYHQWATGSEGTLVQRVTGVWRSAFSVDTPEQCRAALQKLQDVKYPSATSNTPFRQLLLHVCHDLTLAMLSQQAASWEEAVPALLQAVVRSYTSGNFTLMQEIYSAFLPGGCDHLRDKLGDLSPTMAAFKSLEAFFIYGQLYEVWWSLCGPGSESSIWVWSAESTVSDKQSKPGDSASAEDMEQPLGPGLRLSAESERLLSACKELFSERHASLQTSQRTVAEVQETLAEMIRQHQKSQLCKATANGPNRDEPSQQAERAPSQPPSPTREERNAPVSLPELTRRLTEANERIAEFPESVKAWPFPDVLECCLVLLHIGSQCPGAVNPEMQQQAQKLLQKYGHTRAYRRHCQIRHT

Target ORF information:

RefSeq Version	XM_006246400.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus gem (nuclear organelle) associated protein 5 (Gemin5), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006246400.2

ORF Insert Sequence:

ATGAAACCAG AGCCGCGGAC GCTTCCGCCG TCCCCCAACT GGTACTGCGC GCGCTGCAGC 
GACGCGGCGC CCGGAGGCAT CTTTGGCTTC GCTGCGCGGA CCTCCGTCTT CCTCGTCCGC 
GTGGGCCCGG GCGCCGGCGC GAGCCCAGGG GCGCCCCCGT TTCGAGTTGT AGGAGAGTTG 
GTGGGACACA CTGAAAGAGT ATCTGGTTTC ACTTTTTCTC ATCACCCTGG ACAATACAAC 
CTCTGTGCCA CCAGCTCAGA TGATGGGACT GTGAAGATTT GGGATGTAGA GACCAAGGCT 
GTAGTGACAG AACACGCGCT CCATCAGCAT ACAATATCAG CACTGCATTG GTCTCCGACA 
GTTAAGGACT TAATAGTCTC TGGGGATGAA AAAGGAGTTG TTTTCTGTTA CTGGCTTAAC 
AGAAGTGACA GCCAGCACCT CTTCACAGAG CCCAGGGCCA TATTCTGTCT GACCTGTTCA 
CCTCATCACG AAAATCTAGT AGCCATTGGC TACAAAGACG GCATAGTGAT TATCATTGAC 
ATCAGTAAAA AGGGAGAAGT TGTTCACAGG CTTCGAGGGC ACGACGATGA AATCCACTCC 
ATAGCCTGGT GTCCTTTGTC TGGTGAAGAC TGCTTATCTA TAAACCAAGA GGAAAACTCA 
GAACCTGACA TTCCCAATGG GAAGCTTATA GCAGAAACCC CCATCACAAA AGGCTGCTAC 
TTAGCTACAG GAAGCAAGGA TCAGACCATT CGAATCTGGA GCTGTTCCCG AGGCCGGGGT 
GTAATGGTTT TGAAATTGCC CTTTCTGAAG AGAAGAAGTG GGGGTGTGGA CCCAACAGTT 
AAAGAACGTC TTTGGTTGAC ACTTCATTGG CCCAAGAACC AACCAACACA GCTGGTATCC 
AGTTGTTTTG GAGGTGAGCT GCTGCTGTGG GACCTCACCC AGTCATGGAG ACGGAAGTAC 
ACGCTCTTCA GCACCTCAGC GGAAGGGCAC AATCACTCCA GAATTGTCTT CAACTTGTGT 
CCTTTGAAGA CTGAGGATGG CAAACAGCTG CTATTGTCCA CGTCCATGGA CAGAGATGTA 
AAATGTTGGG ACATGGCCAC CCTGGAGTGT TGCTGGACAT TACCTTCTCT TGGAGGTTTT 
GCCTACAGCC TGGCCTTTTC ACCTGTGGAC GTGGGCTCCC TGGCCATAGG TGTTGGGGAC 
GGCATGATCC GGGTATGGAA TACACTCTCC ATAAAGAACA ACTACGATGT GAAAAACTTC 
TGGCAAGGAG TCAAGTCCAA GGTCACGGCG CTATGCTGGC ATCCAAACAA GGAGGGCTGC 
TTAGCCTTTG GGACCGATGA CGGAAAAGTG GGATTGTATG ACACCTGCTC CAACAAACCT 
CCACAGATTT CAAGCACATA TCATAAGAAG ACCGTATATA GGTTGGCTTG GGGGCCACCA 
GTGCCCCCCA TGTCACTTGG AGGGGAAGGA GACAGGCCTT CCCTCACCTT GTACAGCTGT 
GGAGGGGAAG GCATTGTGCT GCAGCATAAT CCCTGGAAGC TCAGTGGAGA GGCCTTTGAC 
ATCAACAAAC TCATGAGAGA CACCAACTCC ATTAGATACA AGCTGCCTGT GCACACAGAG 
ATCAGCTGGA AAGGAGATGG CAAAGTCATG GCTCTTGGAA ATGAAGATGG ATCAATAGAA 
ATATTCCAAG TCCCAAACTT GAAACTGCTC TGCACCATCC AGCAGCACCA CAAGCTTGTG 
AACGCCATAG TCTGGCACCA TGAGCATGGC AGCCGTCCAG AGCTGAGCTG CCTCCTTGCT 
TCTGGCTCCA ACAATGCAGT TATCTACGTG CACAACCTGA AAGCTGTCCT AGAGAGCAAT 
CCTGAGTCTC CAATAACCAT CACAGAGCCC TACCGGACCC TCTCAGGGCA CACAGCCAAG 
GTTACCAGTC TGGCATGGAG CCCACATCAT GATGGAAGGC TGGTATCTGC TTGCTACGAT 
GGCACAGCTC AGGTGTGGGA TGCCCTCCGA GAAGAACCTT TGTTCAATTT CCGAGGACAT 
CGAGGCCGGC TGTTATGTGT TGCGTGGTCC CCAGTGGATC CAGAATGCAT CTACTCAGGT 
GCAGATGACT TCTGTGTCTA CAGGTGGCTG ACCTCCATGC AGGACCATTC CCGGCCTCCT 
CAAGGGAAAA AATGTATTGA ATTAGAGAAA AAACGGCTCT CTCAATTTAA GCCAAAGCTA 
AAAAAGAAGA AAAAACCTAC CTTGCGACTG CCCATAAAGC AAGATTCATC CATTGGCAAT 
GAAGATGAGA GTGTGAGGGA GAACTTGGCA CCTGTTGAGA ATGGCTTGTC CGATCAGGAT 
GGTGAGGAGG AAGCCCAGGA GCCGGAGCTG CCTCCCTCTC CCGTGGTTTG TGGAGAACCT 
GTTTCCTGCA CTGCTGTCTC CTTAGGCTTG GAAAAGTCAA AAGTCACTGT TACTAGTAAA 
GTCGCCTCAC TGAAAAAGGA GCCACCTAAA GAGAAGCCAG AAGCCTTGCT CAAGAAGAGG 
AAGGCTCGCT CCATGCTCCC CCTGAGCACC AGCTTAGACC ACAGGTCCAA AGAGGAGCTC 
CATCGAGACT GTTTGGTTCT AGCAACGGCA ACACACGCCA GAGAACTGCA TGAAGATGTG 
TCTGCCGACC TTGAGGAACG ATTTCACTTG GGCCTTTTCA CAGACAGGGC TGCTCTGTAC 
AGGATGATGG AGACAGAGGG GAAAGGTCAC TTGGAAAGTG GCCACCCCGA GCTTTTTCAC 
CAGCTCATGC TCTGGAAGGG AGATCTGAAA GGCGTTCTCC AGGCTGCAGC AGAGAGAGGC 
GAGCTCACAG ACAGTCTCGT GGCCGTGGCA CCAGTAGCTG GCTACAGTGT GTGGCTGTGG 
GCTGTGGAAG CCTTTGCCAA GCAGCTGTGT TTCCAGGATC AGTATGTCAA GGCTGCCTCC 
TACCTGCTAT CCATCCACAG AGTGTACGAA GCTGTGGAGC TTCTCAGGTC AAACCATCTC 
TACAGAGAAG CTATTGCAGT TGCCAAGGCT CGGCTGCGCC CTGAGGACCC TGTTCTGAAG 
GACCTGTACC TCAGCTGGGG GTCCATCCTG GAGAGAGATG GCCACTATGC AATTGCAGCC 
AAGTGCTACT TAGGAGCCAC TTCCGCCTAT GATGCAGCCA AAGTCCTAGC CAGAAAGGGA 
GATGCAGCCT CTCTTAGAAC AGCTGCAGAG CTGGCTGCCA TAGCAGGAGA GAGTGAGCTG 
GCTGCTTCCC TGGCTCTTAG GTGCGCCCAA GAGCTGCTTC TGCTGAAGAA CTGGGTGGGT 
GCACAGGAAG CCTTGGGACT GCATGAAAGC CTGCAGGGCC AGAGACTGGT GTTCTGCCTC 
CTTGAGCTTC TATGCCGGCA CCTGGAGGAA AAACAGCCTC CAGAGGTCAG AGGCCCCTCT 
TCCATTTACC ACCAGTGGGC CACTGGTTCT GAGGGAACCT TGGTGCAGAG AGTAACTGGT 
GTGTGGCGCA GCGCCTTCAG TGTGGACACC CCGGAGCAGT GTCGGGCCGC CTTGCAGAAG 
CTACAGGATG TCAAGTACCC ATCAGCAACA AGTAACACTC CCTTCAGACA GCTGCTGCTT 
CATGTCTGCC ATGACCTAAC CTTAGCAATG CTGAGCCAGC AGGCTGCTTC CTGGGAGGAG 
GCTGTCCCTG CACTGCTGCA GGCTGTGGTT CGGAGCTACA CCTCAGGGAA TTTCACCCTC 
ATGCAAGAAA TCTACTCAGC CTTTCTCCCA GGCGGCTGTG ACCACCTACG AGACAAACTA 
GGTGACCTAT CTCCTACCAT GGCGGCTTTC AAAAGCTTAG AGGCCTTTTT CATTTATGGG 
CAACTGTATG AAGTCTGGTG GTCACTCTGT GGGCCTGGCT CAGAATCAAG CATCTGGGTG 
TGGTCGGCTG AAAGCACAGT CTCTGATAAA CAGAGCAAGC CAGGGGACAG TGCCAGTGCT 
GAAGACATGG AGCAGCCTCT AGGCCCAGGC CTGAGGCTCA GTGCGGAGAG TGAACGTTTG 
CTGAGTGCCT GCAAGGAGCT CTTCTCAGAG AGGCATGCCA GCCTACAGAC CTCCCAGAGG 
ACTGTTGCTG AAGTCCAAGA GACGCTGGCA GAGATGATCC GCCAGCACCA AAAGAGTCAG 
CTCTGCAAGG CCACAGCCAA CGGCCCCAAC AGAGATGAAC CCAGCCAGCA AGCAGAGCGG 
GCCCCTTCCC AACCTCCGAG CCCAACCAGA GAAGAAAGAA ATGCACCGGT GTCTCTCCCT 
GAATTAACCA GAAGGCTCAC AGAGGCAAAC GAGAGAATAG CTGAGTTTCC AGAGAGTGTA 
AAGGCCTGGC CCTTTCCAGA TGTGCTGGAG TGCTGTCTCG TCTTACTTCA CATCGGGTCC 
CAGTGTCCTG GTGCTGTGAA CCCTGAGATG CAGCAACAGG CCCAGAAGCT CCTTCAGAAG 
TATGGCCACA CTAGAGCTTA CAGAAGACAC TGCCAGATTC GACACACATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Structural basis for snRNA recognition by the double-WD40 repeat domain of Gemin5.
Genes & development30(21)2391-2403(2016 Nov)
Jin W,Wang Y,Liu CP,Yang N,Jin M,Cong Y,Wang M,Xu RM

Structural insights into Gemin5-guided selection of pre-snRNAs for snRNP assembly.
Genes & development30(21)2376-2390(2016 Nov)
Xu C,Ishikawa H,Izumikawa K,Li L,He H,Nobe Y,Yamauchi Y,Shahjee HM,Wu XH,Yu YT,Isobe T,Takahashi N,Min J

The RNA-binding protein Gemin5 binds directly to the ribosome and regulates global translation.
Nucleic acids research44(17)8335-51(2016 Sep)
Francisco-Velilla R,Fernandez-Chamorro J,Ramajo J,Martinez-Salas E

Gemin5 Binds to the Survival Motor Neuron mRNA to Regulate SMN Expression.
The Journal of biological chemistry290(25)15662-9(2015 Jun)
Workman E,Kalda C,Patel A,Battle DJ

Insights into RNA biology from an atlas of mammalian mRNA-binding proteins.
Cell149(6)1393-406(2012 Jun)
Castello A,Fischer B,Eichelbaum K,Horos R,Beckmann BM,Strein C,Davey NE,Humphreys DT,Preiss T,Steinmetz LM,Krijgsveld J,Hentze MW

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Gemin5 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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