Spen cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Spen
Entrez Gene ID	690911
Full Name	spen family transcriptional repressor
Synonyms	RGD1564662
General protein information	Preferred Names spen family transcriptional repressor Names msx2-interacting protein SPEN homolog, transcriptional regulator rCG30673-like
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 5 Map Location: 5q36

mRNA and Protein(s)

mRNA	Protein	Name
NM_001271495.1	NP_001258424.1	msx2-interacting protein

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	SPEN	XP_003949352.1
	LOC696205	XP_002808320.1
Canis lupus familiaris (dog)	SPEN	XP_005618070.1
Bos taurus (cattle)	SPEN	XP_002694185.1
Rattus norvegicus (Norway rat)	LOC690911	NP_001258424.1
Mus musculus (house mouse)	Spen	NP_062737.2
Homo sapiens (human)	SPEN	NP_055816.2

Gene Ontology
Bibliography

Related articles in PubMed

Insights into RNA biology from an atlas of mammalian mRNA-binding proteins.
Castello A, Fischer B, Eichelbaum K, Horos R, Beckmann BM, Strein C, Davey NE, Humphreys DT, Preiss T, Steinmetz LM, Krijgsveld J, Hentze MW
Cell149(6)1393-406(2012 Jun)

The mRNA-bound proteome and its global occupancy profile on protein-coding transcripts.
Baltz AG, Munschauer M, Schwanh?usser B, Vasile A, Murakawa Y, Schueler M, Youngs N, Penfold-Brown D, Drew K, Milek M, Wyler E, Bonneau R, Selbach M, Dieterich C, Landthaler M
Molecular cell46(5)674-90(2012 Jun)

Large-scale proteomics and phosphoproteomics of urinary exosomes.
Gonzales PA, Pisitkun T, Hoffert JD, Tchapyjnikov D, Star RA, Kleta R, Wang NS, Knepper MA
Journal of the American Society of Nephrology : JASN20(2)363-79(2009 Feb)

RBP-Jkappa/SHARP recruits CtIP/CtBP corepressors to silence Notch target genes.
Oswald F, Winkler M, Cao Y, Astrahantseff K, Bourteele S, Kn?chel W, Borggrefe T
Molecular and cellular biology25(23)10379-90(2005 Dec)

Interaction of the Epstein-Barr virus mRNA export factor EB2 with human Spen proteins SHARP, OTT1, and a novel member of the family, OTT3, links Spen proteins with splicing regulation and mRNA export.
Hiriart E, Gruffat H, Buisson M, Mikaelian I, Keppler S, Meresse P, Mercher T, Bernard OA, Sergeant A, Manet E
The Journal of biological chemistry280(44)36935-45(2005 Nov)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Spen gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Spen cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa11272	NM_001271495.1 Latest version!	Rattus norvegicus spen family transcriptional repressor (Spen), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Spen ( NM_001271495.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_001258424.1 Rattus norvegicus spen family transcriptional repressor (Spen), mRNA.

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CloneID	ORa11272
Clone ID Related Accession (Same CDS sequence)	NM_001271495.1
Accession Version	NM_001271495.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 10836bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-30
Organism	Rattus norvegicus(Norway rat)
Product	msx2-interacting protein
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AAHX01040610.1, AAHX01040609.1 and AAHX01040608.1. On Sep 29, 2012 this sequence version replaced XM_003754112.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMD00052296, SAMD00052297 [ECO:0000350] ##Evidence-Data-END##

ATGGTCCGGG AAACCAGGCA CCTCTGGGTG GGAAACTTAC CGGAGAACGT GCGAGAAGAG 
AAGATCATCG AGCATTTCAA ACGATATGGC CGCGTGGAAA GTGTCAAAAT TCTCCCCAAG 
CGGGGGTCTG AAGGAGGAGT GGCTGCCTTT GTGGATTTTG TGGACATCAA AAGTGCACAG 
AAAGCTCACA ACTCCGTCAA CAAAATGGGA GACAGAGACC TTCGCACGGA TTATAATGAA 
CCAGGCACTA TTCCGAGTGC TGCTCGGGGA CTGGATGATA CAGTTTCCAT AGCATCTCGT 
AGTAGAGAGG TTTCTGGGTT CAGAGGAAGT GGTGGAGGGC CTGCTTATGG CCCCCCACCA 
TCACTTCATG CACGGGAAGG ACGCTATGAG CGGAGACTCG ATGGGGCTTC AGATAACAGG 
GAGCGTGCTT ATGAACATAG TGCCTATGGA CACCATGAAC GGGGGACAGG AGCATTTGAT 
CGGACAAGAC ATTACGATCA GGATTACTAT AGAGACCCTC GAGAGCGGAC TTTACAACAC 
GGGCTCTATT ACACTTCTCG GAGTCGAAGT CCAAACCGCT TTGATGCTCA TGACCCCCGA 
TACGAACCTA GGGCCCGGGA GCAGTTTACA CTGCCCAGTG TGGTACACAG GGATATCTAC 
AGGGACGACC TTACCCGGGA GGTACGAGGC AGAAGGCCAG AGCGCAACTA CCAGCACAGC 
AGGAGTCGCT CACCACATTC ATCCCAGTCT AGAAACCAGT CCCCGCAGAG GCTGGCCAGC 
CAAGCGTCCA GACCCACCAG ATCCCCCAGC GGCAGTGGCT CCAGGAGCAG ATCCTCCAGC 
AGCGACTCCA TCAGCAGCAG CAGCAGCAGC AGCACAGACA GTAGTGATTC CAGCAGCACG 
TCCAGTGACG ACTCCCCAGC CCGCTCCGTC CAGTCAGCGG CAGTTCCCGC ACCCACTTCC 
CAGTTGCTTT CGTCTCTTGA AAAAGATGAG CCCCGGAAAA GTTTTGGGAT CAAAGTTCAG 
AATCTTCCAG TACGCTCTAC AGATACGAGC CTTAAAGATG GCCTTTTCCA TGAATTTAAG 
AAGTTTGGCA AGGTGACTTC AGTGCAGATC CATGGGGCCT CCGAGGAGAG GTACGGCCTG 
GTGTTCTTCC GGCAACAAGA GGACCAAGAA AAAGCACTGA CTGCATCAAA GGGAAAACTC 
TTCTTTGGCA TGCAGATTGA GGTGACTGCA TGGGTAGGAC CAGAAACAGA GAGTGAAAAC 
GAGTTTCGAC CCTTGGACGA GAGGATAGAT GAATTCCACC CCAAAGCAAC GAGAACGCTT 
TTTATTGGGA ACCTTGAAAA AACGACTACT TACCACGACC TTCGAAACAT TTTTCAGCGC 
TTTGGAGAAA TTGTGGACAT AGACATTAAG AAGGTGAACG GAGTCCCTCA GTATGCGTTC 
CTGCAGTACT GTGACATTGC CAGTGTGTGC AAGGCTATCA AGAAGATGGA CGGAGAGTAC 
CTTGGGAATA ACCGGCTCAA GCTGGGTTTT GGAAAGAGCA TGCCTACCAA CTGTGTGTGG 
TTAGATGGGC TTTCTTCAAA CGTGTCTGAT CAGTATTTAA CACGGCATTT CTGCCGATAT 
GGGCCTGTGG TAAAGGTGGT GTTTGACCGC TTGAAAGGCC TGGCTCTGGT TCTCTACAGC 
GAGATTGAGT ACGCGCAAGC CGCTGTCAGG GAGACCAAGG GCAGGAAGAT TGGTGGGAAT 
AAGATTAAGG TGGACTTTGC AAACCGGGAA AGCCAACTGG CATTTTATCA CTGCATGGAG 
AAGTCTGGTC AAGACATGAG AGACTTCTAT GAAATGTTAA CAGAAAGAAG AGAGGAGCGA 
AGAGGATCCT ATGACTACAG TCAAGACCGG ACATACTATG AAAATGTCCG TACTCCGGGC 
GCCTACCCTG AGGACTCCAG GCGGGACTAC CCAGCTCGAG GGAGAGAGCT TTACTCGGAG 
TGGGAGACGT ACCAAGGAGA GTATTACGAG TCCCGATACT ATGATGACCC TCGAGAGTAC 
AGAGAATACA GGAGCGACCC TTATGAACAA GACATTCGGG AATACAGCTA TAGGCAGAGA 
GAGCGGGAGC GAGAACGGGA GAGATTTGAA TCTGACCGAG ACCATGAGCG GAGGCCCATT 
GAGCGAAGTC AGAGTCCCGT GCACTTGCGT CGCCCACAGA GTCCCGGGGT GTCTCCTGCA 
CATTCGGAGA GGTTGCCGAG TGACTCTGAG AGGAGACTGT ACCGCCGGTC CTCAGAGAGA 
AGTGGAAGCT GCAGCTCGGT GTCTCCCCCA CGGTACGATA AGCTGGAGAA GGCACGGTTG 
GAGCGCTATA CAAAAAATGA AAAGACAGAT AAAGAACGAA TTTTTGACGC TGAGCGGGTG 
GAGAGGGAGA GGCGAGTAAT CCGCAAGGAG AAAGTAGAGA AGGACAAACC CGAGAGGCAG 
AAACGGAAAG GAAAGGTGCA CTCCCCTAGC TCTCAGTCTT CAGAAACAGA GCAGGAGAAC 
GACCGAGATC AGAGCCCTGA GAAACCACGG GCTTCCACCA AACTGAGCAG AGACAGGACT 
GACAAGGAGG GGCCAGCCAA GAACCGCCTG GAGCTGGTGC CTTGTGTGGT TTTGACCCGA 
GTAAAGGAAA AGGAGGGTAA GGTCATCGAC CACCCGCCCC CAGAGAAGCT CAAAGCCAGG 
CTTGCTCACG ACACTGCCAA GTCCTCAGTG CTGGACCAGA AGTCCCAGGC TTCTCAGGTG 
GAGCCTCCTA AGGCAGACTC GTCTAAGGTG GACGCTGCCA GGGGGAAAGC GCTGAGGGAA 
AAGGCTTTTT CAAGCCACGC AGAGGTTGTG GATAAGGAGG GCAGGCCAAA GCTCAGGAAG 
CACCTCAAGC CCGAACAGGC GCCTGAGCTG AGTGCCCTGG ACCTGGAGAA GCTTGAAGCA 
AGGAAAAGGC GCTTTGCAGA TTCCAGCCTG AAAGTAGAAA AGCCAAGACC AGAGGTCAGG 
AAAACCAGCC CTGAGGCAGA GGACACTCGA GCGCTGTTAA AGAAGCAGCC TGACACACCG 
AGGGATGGTA TTTTGCTGAG GGAAGGAGAG TCTGAAAGGA AGCCTGTGAG AAAAGAAATC 
CTTAAAAGGG AGTCAAAAAA AACCAAGCTG GAGAGACTTA ATTCAACCCT CAGCCCCAAA 
GACTGCCAGG AACCTGCTGC TGTCTCTGCG GGGTCTGGCT CTCGGCCTAG CTCAGATGTC 
CACGCAGCCC TGGGAGAACC AGTACATGGA TCTGTGGAGA CCCAGGAAGC CCAGCCCAAA 
AAGGTCATGC CCTCAAAACC TCGGCTCAAA CAGCTACAGC TCCTGGATAG CCAAGGGCCC 
GAGAAGGAGG AAGTGAGGAA AAACTACTGC CGGCTTCGCG AGGATCCAGT GGAGCACAAA 
GCAGGCCAGG AGAAGCCTCA TGGAGTAAAT GCTGAGGAGA AACTTGGCAT TGACATCGAT 
CACACGCAGA GCTACCGAAA GCAGATGGAG CAGAGTCGCC GGAAGCAGCA GATGGAGATG 
GAGATAGCAA AGTCTGAGAA GTTCGGCAGC CCCAAGAAAG ATGTGGATGA GTATGAAAGG 
CGCAGCCTTG TGCACGAGGT GGGCAAGCCC CCTCAGGATG TCACCGACGA CTCCCCGCCC 
AGCAAAAAGC GAAGGACAGA TCACGTGGAT TTCGATATCT GTACCAAAAG AGAGAGAAAT 
TACCGAAGTT CACGGCAGAT CAGCGAGGAC TCGGAAAGAA CCAGCTGTTC CCCCGGCGTC 
CACCACGGCT CCTTTCATGA AGATGATGAT CCCATAGGCT CGCCTAGGTT AATGTCAGTG 
AAAGGGTCCC CTAGAGTGGA TGACAAAGGT CTCCCCTATT CTAATGTAGC AGTGAGAGAA 
GACCCGTTCA GATGTACTCC TTATGATTCT GGCAAGAGAG AACAGGCAGC AGACATGGCC 
AAAATAAAGC TGTCTGTCTT GAGTTCTGAA GGTGAGCCAA ACCGTTGGGA CCCTCAGATG 
AAACAGGACC CCAGCCGCTT TGATGTCAGT TTCCCAAACA GCATAATTAA AAGAGACAGC 
CTGCGAAAGA GGTGTGTGCG CGATCTGGAG CCTGGCGAGG TGCCCTCTGA TTCTGACGAG 
GACGCCGAGC ACAGAGCCCA GTCACCCAGA GCTTCCAGCT TCTACGACAG CCCTCGGCTG 
TCTTTTTTAT TGAGGGACAG AGACCATAAA CTACGTGACC GAGATGACAG ACTCTCTAGT 
TCTTTAGAAA GAAATAAATT TTATTCTTTT GCACTGGATA AGACAATCAC ACCAGACACT 
AAGGCTTTGC TTGAAAGAGC CAAATCCCTC TCGTCCTCCC GTGAAGAAAA CTGGTCTTTC 
CTCGACTGGG GCTCCCGCTT TGCTAATTTT CGAGACAACA AAGATAAAGA GAAGGTTGAC 
TCCGCCCCAA GACCGATTCC ATCCTGGTAC ATGAAGAAGA AGAAAATCAG GACGGACTCA 
GAGGGGAAAC TGGACGACAA GAAGGACGAG CACAGGGAAG AGGAGCAGGA GCGGCAGGAG 
TTATTTGCCT CTCGGTTCTT ACACAGCTCC ATCTTTGAAC AAGATTCCAG GCGGCTGCAG 
CACCTTGAGA GAAAGAGCGA GGAGTCTGAC TTCCCTCCCG GGCGGCTGTA CGGCAGGCAG 
GCATCTGAGG GTGCGAACAG CACAAGTGAC TCAGTCCAGG AGCCAGTGGT TCTGTTCCAT 
AGCAGATTCA TGGAGCTCAC TCGGATGCAG CAGAAGGAGA AGGAGAAGGA CCAGAAGCCC 
AAAGAGACTG AGAAACAGGA GGGTCCAGAG ACTCCCAAGA CTCCAGAACC CACCACTGAG 
ACTAAAGAGC CGGAGCCCAA AGCCCCTGTT TCCGTTGCAC TTCCTGCCAT CACCGCTGTG 
ACGCCAGAAC CACCGCCACC AGCAGCAGCA GCAGAGAAGG CTGAGGAAAC ATCAGAGGCT 
CCTTCACTGG CAGGAGAGAG GCCCGAGGAG CCCACTCCTG TCTTGGAAGA AACAAAGCTT 
GTGTCTGAGC CGCCTTCTGA ATCCGTGGAA CAGCGCAAGC AGGTGGACTT ATCCCCTGGA 
AAGGACAGCC ATGACAGCAC TGCTGTGGTA GCCTCCACTC CTCCAGAAAG CACAACTACT 
GACACTGTCC CTTGTGTGGA CGCCGAGCCT CTGACTCCCG GAACCACAGT TTCCCAGGTA 
GAGAGCAGTG CAGACCCCAA ACCCAGCAGT CCCCAGCCCC CTTCAAAACT GACTCAGAGG 
TGTGAAGAAG CTGAAGAGCC TAAAGTGGAG AGGCCAGAAA CAACTGCAAG CATTGAACCT 
GACGCCACTG AGAAAGCCGA AGTCGTGCCT GAAGTTCAGC CCCCAGCTTC TGAGGATGTG 
GAAGCCAGTC CTCCCGTCAC AGCAAAAGAC CGGAAGACGA CCAAAAGCAA GCGTTCCAAG 
ACATCAGTCC CGGCAGCTGC CGCAAGCATC GTGGAGAAGC CTGTCACCAG GAAGAGCGAG 
AGGATAGACC GGGAGAAGAT GAAGCGGTCC AGCTCTCCTC GTGGAGAAGC ACAGAAGCTG 
TTAGAACTCA AGATGGAGGC AGACAAGGTC ACGAGGACTG CGTCTAAAAG CTCTGGGGGC 
GACACAGAGC ACCCTGAGCC TAGCTTGCCT CTCAGCAGAA CCAGGCGCCG AAATGTAAGG 
AGTGTTTACG CCACCATGGG TGACCACGAA AGCCGGTCTC CAGCCAAAGA GCCTACTGAG 
CAACCCAGAG TGACCAGGAA GAGATTGGAG CGGGAGCTGC AGGAGGCTGT GGTAGTTCCC 
GCCACCCCTC GGAGGGGAAG GCCTCCTAAA ACGCGCCGGC GTGTCGAAGA GGGCGGTGAG 
CAGGAAAGAA AAGAACCAGC AGACACACCC AGGCCTGCTG AGGGGCGGAG GTCTCCCAGG 
TCTCAAAAAT TGGCAGCTAC AGGTGGGCCC CAAGGGAAAA GGGGGCGGAA TGAGCACAGG 
GCCGAGGCCG CTGAGGCCAC TGCCCAGGTG AGCGCGAGAG AAGGGAACGC TAAGGCCAGG 
GGCGATAGAG AAGCAGCAAG TGAGACAAGG CGCGACAGGA AAGACCCCAG CACAGACAAA 
GACGGGCCTG ACATCTTCCC AGTTGAGGTA GAGAGAAAAC CTCCAGAGAA AACCTACAAA 
TCAAAGAGAG GCCGTGCTCG TAGCACCAGG TCAGGGATGG ACAGAGCTGC ACATCTGAAA 
AGTCTGGAGA TGGCCAGGCA GGCAGCAGAC AAGGAGGCAG AGCCTGTGGC CGCCTCCCCT 
GAGAAAAACG AGAGCCCCCT GGAGGGAAGT GGCGTCTCTC CTCAGCTGGC AGACAACCCA 
GCCGACCCTG ACAAGGAAGC AGAGAAGGAA AGTGTGTCTG CCTCCACGGT GTCCCCAGAA 
GCCAGGCAGC TAACCAAGCA GATGGAGCTG CAGCAGGCTG TGGAGAACAT TGCAAAGCTG 
GCCGAGCCCT CAGCTGCTGC AGCTGACAAG GGCACAGCCA CAGCCACGTC TGAAGAGCCC 
GCCGCTGAGG ATGGCCACAA GCCGGCACAC CAGGCAAGCG AAACTGAATT GGCTGCGGCC 
ATTGGCTCCA TCATCAGTGA CAGCTCTGGA GAGCCAGAAA ACTTCTCTGC TTCTCCCTCT 
GTCCCCCCAG GATCTCAGAC ACATCTCCAG GAAGGAATGG AGCCAGGGCC CCACGAGGCC 
ACACCAGGAA TCTTGGAGAC AGAGGCAGCA ACAGAGTCTT CTGCCCAACA AGCCAGTGCC 
TCGGACCCAC CAGCAGGCTC TGCGGACACC AAGGAAGCCA GAGGAAATAG CAGTGACTCT 
GTGCAGCAAG CCAAGGAGGC TAAAGTGGCA GTCACTCCTC CCCGGAAAGA CAAAGGGCGC 
CAAAAGACGA CACGATCACG CCGGAAAAGG AACGTTAACA AGAAAGTGGC CGCTGCCACA 
GAGACTCGTG CCTCTGAGGC TGAGCAGCCA CAAAGTGAGA GCCCTGCTGC TGTGGTGGTG 
GCAGCAGCAC CCCCAGAAGA GCCCCAGGAA GAAAAGCAGA GTGAGAAGCC TCACTCCCCT 
CTCAGGGAGT GCACTTCTGA CCCCAGCAAG ACCCCACCTG CTGAGAGTCT GTCCCAGGAA 
AACAGTGTTG AAGAAAAGAC TCCATGCAAA ACCCCTGCCC CCCCTGACCT GCCCCCCCTT 
TCACAGCCAG CACTGGTGGA TGATGAACCC CAAGCCAGGT TCAAGGTGCA TTCAATCATC 
GAAAGTGATC CAGTGACCCC ACCCAGTGAC TCCAGCATAC CTCCACCCAC AATTCCTTTA 
GTGACCATAG CAAAGCTCCC ACCTCCTGTC ATCCCTGGGG GAGTCCCACA TCAGAGTCCC 
CCTCCTAAGG TGACAGAGTG GATCACAAGA CAGGAAGAGC CTCGGGCTCA GTCTGCCCCC 
TCACCGGCTC TTCCCCCAGA CACAAAGGCT TCTGATGTAG ACACCAGCTC CAGTACACTG 
AGGAAGATCC TCATGGACCC CAAATATGTG TCTGCCACAG GTGTTACTTC CACAAGTGTC 
ACTGCGGCCA TTGCAGAGCC TGTGAGTGCC CCTCGTCTGC AGGAGGCGCC GGCCCCTCCC 
TGTGAACCTA AGCGCCCTTC CTCAGAAGAG AAGGTAGCAG CCGCAGTACC ACATGCCGAC 
ACACAGGCCT CAGAGGTTCC AGTCGCTGCT GACAAAGAGA AGGTGGCCCC TGTCATCGCC 
CCTAAAATCA CATCAGTTAT TAGCCGGATG CCTGTTAGCA TCGACCTGGA GAACTCACAG 
AAGATAACGT TGGCAAAGCC GGCTCCGCAG ACCCTGACCG GCCTGGTGAG CGCCCTAACT 
GGCCTGGTGA ATGTCTCTCT GGTCCCAGTG AATGCCTTAA AGGGGCCTGT GAAGGGCTCT 
GTAGCCACCC TGAAAGGTTT GGTGAGCACC CCTGCTGGGC CTGTGAATCT CCTAAAGGGG 
CCCGTGAATG TCCTCACCGG TCCTGTCAAC GTCCTGACCA CTCCAGTGAG TGCCACGGTG 
GGCACAGTGA GCGCTGCCCC TGGTACCGTG ACTGCTGCCT GTGGTGTGAC TGCCACTACA 
GGCACAGCAG CCATGTCGGG TGCAGTGCCT GCCCCAGCAG CAAAGGGCAA GCAGAGGTCA 
AGCAGCAACG AGAACAGCCG CTTCCACCCT GGGTCAATGT CCGTCATCGA TGACCGCCCA 
GCAGACACAG GCTCTGGCGC AGGGCTGCGT GTGAATACTT CAGAAGGGGT CGTGCTCCTG 
AGCTACTCAG GACAGAAGAC GGAAGGCCCT CAGCGCATCA GTGCTAAGAT CAGCCAGATC 
CCTCCGGCCA GTGCAATGGA CATCGAGTTC CAGCAGTCAG TATCGAAGTC CCAGGTGAAA 
GCCGACTCCG TCACCCCGAC CCAGGCTGCA CCCAAAGGCC CTCAGACCCC TTCTGCCTTT 
GCAAACGTGG CTGCCCACTC TACCCTGGTC CTGACTGCCC AGACATACAA TGCCTCTCCC 
GTGATCTCCT CTGTGAAGAC CGACCGTCCA TCCTTGGAGA AGCCCGAGCC CATTCACCTC 
TCCGTGTCCA CCCCTGTCAC CCAGGGGGGC ACAGTGAAGG TTCTCACCCA GGGCATCAAC 
ACACCCCCAG TGCTGGTTCA CAACCAGCTT GTCCTCACCC CAAGCATCGT CACCACTAAC 
AAGAAACTTG CTGACCCTGT CACTCTGAAA ATAGAGACCA AGGTCCTTCA GCCGGCTAAC 
CTGGGGCCCA CCCTCACTCC CCACCACCCC CCTGCCCTTC CCAGCAAACT GCCGGCCGAA 
GTGAACCATG TTCCTTCCGG ACCCAGCAAC CCAGCAGATC GAACCATTGC CCACTTGGCA 
ACCACAAAAC CAGATACTCA TTCTCCTCGA CCCACTGGGC CTACACCAGG CCCATTCCCA 
AGGGCCTGCC ATCCCAGCAG CACGACATCC ACAGCGCTTT CCACCAATGC AACTGTCATG 
CTGGCTGCAG GCATTCCAGT GCCACAGTTC ATCTCCAGCA TACACCCAGA GCAGTCTGTC 
ATCATGCCCC CCCACAGCAT CACTCAGACC GTGTCTCTCG GTCACCTGTC CCAAGGCGAG 
GTGAGAATGA GCACACCCAC CTTGCCCAGC ATCACCTACA GCATCCGGCC AGAGACCCTG 
CACTCTCCTC GGGCCCCTCT GCAGCCCCAA CAGATAGAAG CAAGGGCCCC ACAGCGTGTG 
AGCACGCCCC AGCCAGCCAC AACTGCTGTG CCCGCGCTGG CCACACAGCA CCCTCCCGAG 
GAAGAAGTTC ACTACCACCT CCCTGTGGCT CGAGCTGCAG CCCCTGTGCA GTCAGAAGTG 
CTGGTCATGC AGTCCGAGTA CCGGTTACAC CCATACACCG TGCCCCGAGA CGTCCGGATC 
ATGGTGCATC CACATGTGAC TGCTGTCAGT GAACAGCCCA GGGCCACAGA GGGCGTGGTG 
AAGGTGCCAC CAGCCAGCAA GGCCCCCCAG CAACTGGTGA AAGAAGTGGT TAAGACTTCA 
GATGCCAAAG CAGTCCCAGC TCCAGCCCCC GTCCCGGTCC CTGTCCCTGT CCCGACCCCT 
GCCCCACCCC CTCATGGTGA GGCCCGAATT CTCACAGTCA CCCCCAGCAG CCAGCTTCAG 
GGGCTGCCTC TGACCCCGCC TGTGGTGGTA ACCCACGGGG TGCAGATTGT GCACTCCAGC 
GGGGAGCTGT TCCAAGAGTA TAGGTATGGC GACATCCGTA CCTACCACGC CCCTGCTCAG 
CTCACACATA CTCAGTTTCC TGTCGCCTCT TCCATCAACC TACCCTCCAG GACCAAGACC 
TCGGCCCAGG GCCCCCCCGA AAGTGAGCCC TTGCAGTCTA CGCAGCCTGC ACAGCCTGCC 
CCATCTACGC AGTCCACACA GCCTGTACCA CCTGCCCCAC CCTGCCAGCC CTCCCAGCTC 
AGCCAGCCTG CCCAGCCACC AAGTGGCAAG ATTCCTCAGG TCTCCCAAGA AGCCAAGGGG 
ACACAGACAG GAGGGATAGA GCAGACTCGC CTCCCAGCCA TGCCCACGAA CAGGCCGTCT 
GAGCCACATG CTCAGGCTCA GAGGGCACCG GGGGAAACCC CCCAACCAGC CCATCCCTCC 
CCCGTGTCAG TCGCCATGAA GCCGGACCTC CCAACCCCTC TTGCCTCTCA GGCTGCCCCA 
AAGCAGCCAT TGTTTGTCCC CGTAAACTCA GGCACGAGCA CCCCACCCGG ACTGGCTCTG 
CCTCATGCTG AAGCCCAGCC AGCCCCAAAG CAAGACTCTG CTCCACACGG GACACCACAA 
AGGCCCGTGG ATATGGTCCA GCTTCTGAAG AAGTACCCCA TCGTGTGGCA GGGCCTGCTG 
GCCCTCAAGA ACGACACAGC TGCTGTGCAG CTCCACTTTG TCTCTGGCAA CAACGTCCTG 
GCTCACCGCT CCCTGCCCCT CTCTGAAGGC GGCCCCCCGC TGCGGATCGC CCAAAGGATG 
CGACTGGAGG CCTCGCAACT GGAAGGAGTT GCCAGGAGGA TGACAGTGGA AACAGACTAC 
TGCCTGTTGC TGGCTCTGCC CTGTGGCCGT GACCAGGAGG ATGTCGTAAG CCAGACCGAG 
TCCCTCAAGG CTGCCTTTAT CACTTACCTG CAGGCCAAGC AAGCAGCAGG GATTATCAAT 
GTCCCCAATC CTGGCTCCAA TCAGCCCGCC TATGTGCTGC AGATCTTCCC ACCCTGTGAG 
TTCTCCGAGA GCCACCTCTC CCGGCTGGCC CCTGACCTCC TTGCCAGCAT CTCCAACATC 
TCTCCCCACC TCATGATTGT CATTGCCTCA GTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001258424.1
CDS	1..10836
Translation	MVRETRHLWVGNLPENVREEKIIEHFKRYGRVESVKILPKRGSEGGVAAFVDFVDIKSAQKAHNSVNKMGDRDLRTDYNEPGTIPSAARGLDDTVSIASRSREVSGFRGSGGGPAYGPPPSLHAREGRYERRLDGASDNRERAYEHSAYGHHERGTGAFDRTRHYDQDYYRDPRERTLQHGLYYTSRSRSPNRFDAHDPRYEPRAREQFTLPSVVHRDIYRDDLTREVRGRRPERNYQHSRSRSPHSSQSRNQSPQRLASQASRPTRSPSGSGSRSRSSSSDSISSSSSSSTDSSDSSSTSSDDSPARSVQSAAVPAPTSQLLSSLEKDEPRKSFGIKVQNLPVRSTDTSLKDGLFHEFKKFGKVTSVQIHGASEERYGLVFFRQQEDQEKALTASKGKLFFGMQIEVTAWVGPETESENEFRPLDERIDEFHPKATRTLFIGNLEKTTTYHDLRNIFQRFGEIVDIDIKKVNGVPQYAFLQYCDIASVCKAIKKMDGEYLGNNRLKLGFGKSMPTNCVWLDGLSSNVSDQYLTRHFCRYGPVVKVVFDRLKGLALVLYSEIEYAQAAVRETKGRKIGGNKIKVDFANRESQLAFYHCMEKSGQDMRDFYEMLTERREERRGSYDYSQDRTYYENVRTPGAYPEDSRRDYPARGRELYSEWETYQGEYYESRYYDDPREYREYRSDPYEQDIREYSYRQRERERERERFESDRDHERRPIERSQSPVHLRRPQSPGVSPAHSERLPSDSERRLYRRSSERSGSCSSVSPPRYDKLEKARLERYTKNEKTDKERIFDAERVERERRVIRKEKVEKDKPERQKRKGKVHSPSSQSSETEQENDRDQSPEKPRASTKLSRDRTDKEGPAKNRLELVPCVVLTRVKEKEGKVIDHPPPEKLKARLAHDTAKSSVLDQKSQASQVEPPKADSSKVDAARGKALREKAFSSHAEVVDKEGRPKLRKHLKPEQAPELSALDLEKLEARKRRFADSSLKVEKPRPEVRKTSPEAEDTRALLKKQPDTPRDGILLREGESERKPVRKEILKRESKKTKLERLNSTLSPKDCQEPAAVSAGSGSRPSSDVHAALGEPVHGSVETQEAQPKKVMPSKPRLKQLQLLDSQGPEKEEVRKNYCRLREDPVEHKAGQEKPHGVNAEEKLGIDIDHTQSYRKQMEQSRRKQQMEMEIAKSEKFGSPKKDVDEYERRSLVHEVGKPPQDVTDDSPPSKKRRTDHVDFDICTKRERNYRSSRQISEDSERTSCSPGVHHGSFHEDDDPIGSPRLMSVKGSPRVDDKGLPYSNVAVREDPFRCTPYDSGKREQAADMAKIKLSVLSSEGEPNRWDPQMKQDPSRFDVSFPNSIIKRDSLRKRCVRDLEPGEVPSDSDEDAEHRAQSPRASSFYDSPRLSFLLRDRDHKLRDRDDRLSSSLERNKFYSFALDKTITPDTKALLERAKSLSSSREENWSFLDWGSRFANFRDNKDKEKVDSAPRPIPSWYMKKKKIRTDSEGKLDDKKDEHREEEQERQELFASRFLHSSIFEQDSRRLQHLERKSEESDFPPGRLYGRQASEGANSTSDSVQEPVVLFHSRFMELTRMQQKEKEKDQKPKETEKQEGPETPKTPEPTTETKEPEPKAPVSVALPAITAVTPEPPPPAAAAEKAEETSEAPSLAGERPEEPTPVLEETKLVSEPPSESVEQRKQVDLSPGKDSHDSTAVVASTPPESTTTDTVPCVDAEPLTPGTTVSQVESSADPKPSSPQPPSKLTQRCEEAEEPKVERPETTASIEPDATEKAEVVPEVQPPASEDVEASPPVTAKDRKTTKSKRSKTSVPAAAASIVEKPVTRKSERIDREKMKRSSSPRGEAQKLLELKMEADKVTRTASKSSGGDTEHPEPSLPLSRTRRRNVRSVYATMGDHESRSPAKEPTEQPRVTRKRLERELQEAVVVPATPRRGRPPKTRRRVEEGGEQERKEPADTPRPAEGRRSPRSQKLAATGGPQGKRGRNEHRAEAAEATAQVSAREGNAKARGDREAASETRRDRKDPSTDKDGPDIFPVEVERKPPEKTYKSKRGRARSTRSGMDRAAHLKSLEMARQAADKEAEPVAASPEKNESPLEGSGVSPQLADNPADPDKEAEKESVSASTVSPEARQLTKQMELQQAVENIAKLAEPSAAAADKGTATATSEEPAAEDGHKPAHQASETELAAAIGSIISDSSGEPENFSASPSVPPGSQTHLQEGMEPGPHEATPGILETEAATESSAQQASASDPPAGSADTKEARGNSSDSVQQAKEAKVAVTPPRKDKGRQKTTRSRRKRNVNKKVAAATETRASEAEQPQSESPAAVVVAAAPPEEPQEEKQSEKPHSPLRECTSDPSKTPPAESLSQENSVEEKTPCKTPAPPDLPPLSQPALVDDEPQARFKVHSIIESDPVTPPSDSSIPPPTIPLVTIAKLPPPVIPGGVPHQSPPPKVTEWITRQEEPRAQSAPSPALPPDTKASDVDTSSSTLRKILMDPKYVSATGVTSTSVTAAIAEPVSAPRLQEAPAPPCEPKRPSSEEKVAAAVPHADTQASEVPVAADKEKVAPVIAPKITSVISRMPVSIDLENSQKITLAKPAPQTLTGLVSALTGLVNVSLVPVNALKGPVKGSVATLKGLVSTPAGPVNLLKGPVNVLTGPVNVLTTPVSATVGTVSAAPGTVTAACGVTATTGTAAMSGAVPAPAAKGKQRSSSNENSRFHPGSMSVIDDRPADTGSGAGLRVNTSEGVVLLSYSGQKTEGPQRISAKISQIPPASAMDIEFQQSVSKSQVKADSVTPTQAAPKGPQTPSAFANVAAHSTLVLTAQTYNASPVISSVKTDRPSLEKPEPIHLSVSTPVTQGGTVKVLTQGINTPPVLVHNQLVLTPSIVTTNKKLADPVTLKIETKVLQPANLGPTLTPHHPPALPSKLPAEVNHVPSGPSNPADRTIAHLATTKPDTHSPRPTGPTPGPFPRACHPSSTTSTALSTNATVMLAAGIPVPQFISSIHPEQSVIMPPHSITQTVSLGHLSQGEVRMSTPTLPSITYSIRPETLHSPRAPLQPQQIEARAPQRVSTPQPATTAVPALATQHPPEEEVHYHLPVARAAAPVQSEVLVMQSEYRLHPYTVPRDVRIMVHPHVTAVSEQPRATEGVVKVPPASKAPQQLVKEVVKTSDAKAVPAPAPVPVPVPVPTPAPPPHGEARILTVTPSSQLQGLPLTPPVVVTHGVQIVHSSGELFQEYRYGDIRTYHAPAQLTHTQFPVASSINLPSRTKTSAQGPPESEPLQSTQPAQPAPSTQSTQPVPPAPPCQPSQLSQPAQPPSGKIPQVSQEAKGTQTGGIEQTRLPAMPTNRPSEPHAQAQRAPGETPQPAHPSPVSVAMKPDLPTPLASQAAPKQPLFVPVNSGTSTPPGLALPHAEAQPAPKQDSAPHGTPQRPVDMVQLLKKYPIVWQGLLALKNDTAAVQLHFVSGNNVLAHRSLPLSEGGPPLRIAQRMRLEASQLEGVARRMTVETDYCLLLALPCGRDQEDVVSQTESLKAAFITYLQAKQAAGIINVPNPGSNQPAYVLQIFPPCEFSESHLSRLAPDLLASISNISPHLMIVIASV

Target ORF information:

RefSeq Version	NM_001271495.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus spen family transcriptional repressor (Spen), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001271495.1

ORF Insert Sequence:

ATGGTCCGGG AAACCAGGCA CCTCTGGGTG GGAAACTTAC CGGAGAACGT GCGAGAAGAG 
AAGATCATCG AGCATTTCAA ACGATATGGC CGCGTGGAAA GTGTCAAAAT TCTCCCCAAG 
CGGGGGTCTG AAGGAGGAGT GGCTGCCTTT GTGGATTTTG TGGACATCAA AAGTGCACAG 
AAAGCTCACA ACTCCGTCAA CAAAATGGGA GACAGAGACC TTCGCACGGA TTATAATGAA 
CCAGGCACTA TTCCGAGTGC TGCTCGGGGA CTGGATGATA CAGTTTCCAT AGCATCTCGT 
AGTAGAGAGG TTTCTGGGTT CAGAGGAAGT GGTGGAGGGC CTGCTTATGG CCCCCCACCA 
TCACTTCATG CACGGGAAGG ACGCTATGAG CGGAGACTCG ATGGGGCTTC AGATAACAGG 
GAGCGTGCTT ATGAACATAG TGCCTATGGA CACCATGAAC GGGGGACAGG AGCATTTGAT 
CGGACAAGAC ATTACGATCA GGATTACTAT AGAGACCCTC GAGAGCGGAC TTTACAACAC 
GGGCTCTATT ACACTTCTCG GAGTCGAAGT CCAAACCGCT TTGATGCTCA TGACCCCCGA 
TACGAACCTA GGGCCCGGGA GCAGTTTACA CTGCCCAGTG TGGTACACAG GGATATCTAC 
AGGGACGACC TTACCCGGGA GGTACGAGGC AGAAGGCCAG AGCGCAACTA CCAGCACAGC 
AGGAGTCGCT CACCACATTC ATCCCAGTCT AGAAACCAGT CCCCGCAGAG GCTGGCCAGC 
CAAGCGTCCA GACCCACCAG ATCCCCCAGC GGCAGTGGCT CCAGGAGCAG ATCCTCCAGC 
AGCGACTCCA TCAGCAGCAG CAGCAGCAGC AGCACAGACA GTAGTGATTC CAGCAGCACG 
TCCAGTGACG ACTCCCCAGC CCGCTCCGTC CAGTCAGCGG CAGTTCCCGC ACCCACTTCC 
CAGTTGCTTT CGTCTCTTGA AAAAGATGAG CCCCGGAAAA GTTTTGGGAT CAAAGTTCAG 
AATCTTCCAG TACGCTCTAC AGATACGAGC CTTAAAGATG GCCTTTTCCA TGAATTTAAG 
AAGTTTGGCA AGGTGACTTC AGTGCAGATC CATGGGGCCT CCGAGGAGAG GTACGGCCTG 
GTGTTCTTCC GGCAACAAGA GGACCAAGAA AAAGCACTGA CTGCATCAAA GGGAAAACTC 
TTCTTTGGCA TGCAGATTGA GGTGACTGCA TGGGTAGGAC CAGAAACAGA GAGTGAAAAC 
GAGTTTCGAC CCTTGGACGA GAGGATAGAT GAATTCCACC CCAAAGCAAC GAGAACGCTT 
TTTATTGGGA ACCTTGAAAA AACGACTACT TACCACGACC TTCGAAACAT TTTTCAGCGC 
TTTGGAGAAA TTGTGGACAT AGACATTAAG AAGGTGAACG GAGTCCCTCA GTATGCGTTC 
CTGCAGTACT GTGACATTGC CAGTGTGTGC AAGGCTATCA AGAAGATGGA CGGAGAGTAC 
CTTGGGAATA ACCGGCTCAA GCTGGGTTTT GGAAAGAGCA TGCCTACCAA CTGTGTGTGG 
TTAGATGGGC TTTCTTCAAA CGTGTCTGAT CAGTATTTAA CACGGCATTT CTGCCGATAT 
GGGCCTGTGG TAAAGGTGGT GTTTGACCGC TTGAAAGGCC TGGCTCTGGT TCTCTACAGC 
GAGATTGAGT ACGCGCAAGC CGCTGTCAGG GAGACCAAGG GCAGGAAGAT TGGTGGGAAT 
AAGATTAAGG TGGACTTTGC AAACCGGGAA AGCCAACTGG CATTTTATCA CTGCATGGAG 
AAGTCTGGTC AAGACATGAG AGACTTCTAT GAAATGTTAA CAGAAAGAAG AGAGGAGCGA 
AGAGGATCCT ATGACTACAG TCAAGACCGG ACATACTATG AAAATGTCCG TACTCCGGGC 
GCCTACCCTG AGGACTCCAG GCGGGACTAC CCAGCTCGAG GGAGAGAGCT TTACTCGGAG 
TGGGAGACGT ACCAAGGAGA GTATTACGAG TCCCGATACT ATGATGACCC TCGAGAGTAC 
AGAGAATACA GGAGCGACCC TTATGAACAA GACATTCGGG AATACAGCTA TAGGCAGAGA 
GAGCGGGAGC GAGAACGGGA GAGATTTGAA TCTGACCGAG ACCATGAGCG GAGGCCCATT 
GAGCGAAGTC AGAGTCCCGT GCACTTGCGT CGCCCACAGA GTCCCGGGGT GTCTCCTGCA 
CATTCGGAGA GGTTGCCGAG TGACTCTGAG AGGAGACTGT ACCGCCGGTC CTCAGAGAGA 
AGTGGAAGCT GCAGCTCGGT GTCTCCCCCA CGGTACGATA AGCTGGAGAA GGCACGGTTG 
GAGCGCTATA CAAAAAATGA AAAGACAGAT AAAGAACGAA TTTTTGACGC TGAGCGGGTG 
GAGAGGGAGA GGCGAGTAAT CCGCAAGGAG AAAGTAGAGA AGGACAAACC CGAGAGGCAG 
AAACGGAAAG GAAAGGTGCA CTCCCCTAGC TCTCAGTCTT CAGAAACAGA GCAGGAGAAC 
GACCGAGATC AGAGCCCTGA GAAACCACGG GCTTCCACCA AACTGAGCAG AGACAGGACT 
GACAAGGAGG GGCCAGCCAA GAACCGCCTG GAGCTGGTGC CTTGTGTGGT TTTGACCCGA 
GTAAAGGAAA AGGAGGGTAA GGTCATCGAC CACCCGCCCC CAGAGAAGCT CAAAGCCAGG 
CTTGCTCACG ACACTGCCAA GTCCTCAGTG CTGGACCAGA AGTCCCAGGC TTCTCAGGTG 
GAGCCTCCTA AGGCAGACTC GTCTAAGGTG GACGCTGCCA GGGGGAAAGC GCTGAGGGAA 
AAGGCTTTTT CAAGCCACGC AGAGGTTGTG GATAAGGAGG GCAGGCCAAA GCTCAGGAAG 
CACCTCAAGC CCGAACAGGC GCCTGAGCTG AGTGCCCTGG ACCTGGAGAA GCTTGAAGCA 
AGGAAAAGGC GCTTTGCAGA TTCCAGCCTG AAAGTAGAAA AGCCAAGACC AGAGGTCAGG 
AAAACCAGCC CTGAGGCAGA GGACACTCGA GCGCTGTTAA AGAAGCAGCC TGACACACCG 
AGGGATGGTA TTTTGCTGAG GGAAGGAGAG TCTGAAAGGA AGCCTGTGAG AAAAGAAATC 
CTTAAAAGGG AGTCAAAAAA AACCAAGCTG GAGAGACTTA ATTCAACCCT CAGCCCCAAA 
GACTGCCAGG AACCTGCTGC TGTCTCTGCG GGGTCTGGCT CTCGGCCTAG CTCAGATGTC 
CACGCAGCCC TGGGAGAACC AGTACATGGA TCTGTGGAGA CCCAGGAAGC CCAGCCCAAA 
AAGGTCATGC CCTCAAAACC TCGGCTCAAA CAGCTACAGC TCCTGGATAG CCAAGGGCCC 
GAGAAGGAGG AAGTGAGGAA AAACTACTGC CGGCTTCGCG AGGATCCAGT GGAGCACAAA 
GCAGGCCAGG AGAAGCCTCA TGGAGTAAAT GCTGAGGAGA AACTTGGCAT TGACATCGAT 
CACACGCAGA GCTACCGAAA GCAGATGGAG CAGAGTCGCC GGAAGCAGCA GATGGAGATG 
GAGATAGCAA AGTCTGAGAA GTTCGGCAGC CCCAAGAAAG ATGTGGATGA GTATGAAAGG 
CGCAGCCTTG TGCACGAGGT GGGCAAGCCC CCTCAGGATG TCACCGACGA CTCCCCGCCC 
AGCAAAAAGC GAAGGACAGA TCACGTGGAT TTCGATATCT GTACCAAAAG AGAGAGAAAT 
TACCGAAGTT CACGGCAGAT CAGCGAGGAC TCGGAAAGAA CCAGCTGTTC CCCCGGCGTC 
CACCACGGCT CCTTTCATGA AGATGATGAT CCCATAGGCT CGCCTAGGTT AATGTCAGTG 
AAAGGGTCCC CTAGAGTGGA TGACAAAGGT CTCCCCTATT CTAATGTAGC AGTGAGAGAA 
GACCCGTTCA GATGTACTCC TTATGATTCT GGCAAGAGAG AACAGGCAGC AGACATGGCC 
AAAATAAAGC TGTCTGTCTT GAGTTCTGAA GGTGAGCCAA ACCGTTGGGA CCCTCAGATG 
AAACAGGACC CCAGCCGCTT TGATGTCAGT TTCCCAAACA GCATAATTAA AAGAGACAGC 
CTGCGAAAGA GGTGTGTGCG CGATCTGGAG CCTGGCGAGG TGCCCTCTGA TTCTGACGAG 
GACGCCGAGC ACAGAGCCCA GTCACCCAGA GCTTCCAGCT TCTACGACAG CCCTCGGCTG 
TCTTTTTTAT TGAGGGACAG AGACCATAAA CTACGTGACC GAGATGACAG ACTCTCTAGT 
TCTTTAGAAA GAAATAAATT TTATTCTTTT GCACTGGATA AGACAATCAC ACCAGACACT 
AAGGCTTTGC TTGAAAGAGC CAAATCCCTC TCGTCCTCCC GTGAAGAAAA CTGGTCTTTC 
CTCGACTGGG GCTCCCGCTT TGCTAATTTT CGAGACAACA AAGATAAAGA GAAGGTTGAC 
TCCGCCCCAA GACCGATTCC ATCCTGGTAC ATGAAGAAGA AGAAAATCAG GACGGACTCA 
GAGGGGAAAC TGGACGACAA GAAGGACGAG CACAGGGAAG AGGAGCAGGA GCGGCAGGAG 
TTATTTGCCT CTCGGTTCTT ACACAGCTCC ATCTTTGAAC AAGATTCCAG GCGGCTGCAG 
CACCTTGAGA GAAAGAGCGA GGAGTCTGAC TTCCCTCCCG GGCGGCTGTA CGGCAGGCAG 
GCATCTGAGG GTGCGAACAG CACAAGTGAC TCAGTCCAGG AGCCAGTGGT TCTGTTCCAT 
AGCAGATTCA TGGAGCTCAC TCGGATGCAG CAGAAGGAGA AGGAGAAGGA CCAGAAGCCC 
AAAGAGACTG AGAAACAGGA GGGTCCAGAG ACTCCCAAGA CTCCAGAACC CACCACTGAG 
ACTAAAGAGC CGGAGCCCAA AGCCCCTGTT TCCGTTGCAC TTCCTGCCAT CACCGCTGTG 
ACGCCAGAAC CACCGCCACC AGCAGCAGCA GCAGAGAAGG CTGAGGAAAC ATCAGAGGCT 
CCTTCACTGG CAGGAGAGAG GCCCGAGGAG CCCACTCCTG TCTTGGAAGA AACAAAGCTT 
GTGTCTGAGC CGCCTTCTGA ATCCGTGGAA CAGCGCAAGC AGGTGGACTT ATCCCCTGGA 
AAGGACAGCC ATGACAGCAC TGCTGTGGTA GCCTCCACTC CTCCAGAAAG CACAACTACT 
GACACTGTCC CTTGTGTGGA CGCCGAGCCT CTGACTCCCG GAACCACAGT TTCCCAGGTA 
GAGAGCAGTG CAGACCCCAA ACCCAGCAGT CCCCAGCCCC CTTCAAAACT GACTCAGAGG 
TGTGAAGAAG CTGAAGAGCC TAAAGTGGAG AGGCCAGAAA CAACTGCAAG CATTGAACCT 
GACGCCACTG AGAAAGCCGA AGTCGTGCCT GAAGTTCAGC CCCCAGCTTC TGAGGATGTG 
GAAGCCAGTC CTCCCGTCAC AGCAAAAGAC CGGAAGACGA CCAAAAGCAA GCGTTCCAAG 
ACATCAGTCC CGGCAGCTGC CGCAAGCATC GTGGAGAAGC CTGTCACCAG GAAGAGCGAG 
AGGATAGACC GGGAGAAGAT GAAGCGGTCC AGCTCTCCTC GTGGAGAAGC ACAGAAGCTG 
TTAGAACTCA AGATGGAGGC AGACAAGGTC ACGAGGACTG CGTCTAAAAG CTCTGGGGGC 
GACACAGAGC ACCCTGAGCC TAGCTTGCCT CTCAGCAGAA CCAGGCGCCG AAATGTAAGG 
AGTGTTTACG CCACCATGGG TGACCACGAA AGCCGGTCTC CAGCCAAAGA GCCTACTGAG 
CAACCCAGAG TGACCAGGAA GAGATTGGAG CGGGAGCTGC AGGAGGCTGT GGTAGTTCCC 
GCCACCCCTC GGAGGGGAAG GCCTCCTAAA ACGCGCCGGC GTGTCGAAGA GGGCGGTGAG 
CAGGAAAGAA AAGAACCAGC AGACACACCC AGGCCTGCTG AGGGGCGGAG GTCTCCCAGG 
TCTCAAAAAT TGGCAGCTAC AGGTGGGCCC CAAGGGAAAA GGGGGCGGAA TGAGCACAGG 
GCCGAGGCCG CTGAGGCCAC TGCCCAGGTG AGCGCGAGAG AAGGGAACGC TAAGGCCAGG 
GGCGATAGAG AAGCAGCAAG TGAGACAAGG CGCGACAGGA AAGACCCCAG CACAGACAAA 
GACGGGCCTG ACATCTTCCC AGTTGAGGTA GAGAGAAAAC CTCCAGAGAA AACCTACAAA 
TCAAAGAGAG GCCGTGCTCG TAGCACCAGG TCAGGGATGG ACAGAGCTGC ACATCTGAAA 
AGTCTGGAGA TGGCCAGGCA GGCAGCAGAC AAGGAGGCAG AGCCTGTGGC CGCCTCCCCT 
GAGAAAAACG AGAGCCCCCT GGAGGGAAGT GGCGTCTCTC CTCAGCTGGC AGACAACCCA 
GCCGACCCTG ACAAGGAAGC AGAGAAGGAA AGTGTGTCTG CCTCCACGGT GTCCCCAGAA 
GCCAGGCAGC TAACCAAGCA GATGGAGCTG CAGCAGGCTG TGGAGAACAT TGCAAAGCTG 
GCCGAGCCCT CAGCTGCTGC AGCTGACAAG GGCACAGCCA CAGCCACGTC TGAAGAGCCC 
GCCGCTGAGG ATGGCCACAA GCCGGCACAC CAGGCAAGCG AAACTGAATT GGCTGCGGCC 
ATTGGCTCCA TCATCAGTGA CAGCTCTGGA GAGCCAGAAA ACTTCTCTGC TTCTCCCTCT 
GTCCCCCCAG GATCTCAGAC ACATCTCCAG GAAGGAATGG AGCCAGGGCC CCACGAGGCC 
ACACCAGGAA TCTTGGAGAC AGAGGCAGCA ACAGAGTCTT CTGCCCAACA AGCCAGTGCC 
TCGGACCCAC CAGCAGGCTC TGCGGACACC AAGGAAGCCA GAGGAAATAG CAGTGACTCT 
GTGCAGCAAG CCAAGGAGGC TAAAGTGGCA GTCACTCCTC CCCGGAAAGA CAAAGGGCGC 
CAAAAGACGA CACGATCACG CCGGAAAAGG AACGTTAACA AGAAAGTGGC CGCTGCCACA 
GAGACTCGTG CCTCTGAGGC TGAGCAGCCA CAAAGTGAGA GCCCTGCTGC TGTGGTGGTG 
GCAGCAGCAC CCCCAGAAGA GCCCCAGGAA GAAAAGCAGA GTGAGAAGCC TCACTCCCCT 
CTCAGGGAGT GCACTTCTGA CCCCAGCAAG ACCCCACCTG CTGAGAGTCT GTCCCAGGAA 
AACAGTGTTG AAGAAAAGAC TCCATGCAAA ACCCCTGCCC CCCCTGACCT GCCCCCCCTT 
TCACAGCCAG CACTGGTGGA TGATGAACCC CAAGCCAGGT TCAAGGTGCA TTCAATCATC 
GAAAGTGATC CAGTGACCCC ACCCAGTGAC TCCAGCATAC CTCCACCCAC AATTCCTTTA 
GTGACCATAG CAAAGCTCCC ACCTCCTGTC ATCCCTGGGG GAGTCCCACA TCAGAGTCCC 
CCTCCTAAGG TGACAGAGTG GATCACAAGA CAGGAAGAGC CTCGGGCTCA GTCTGCCCCC 
TCACCGGCTC TTCCCCCAGA CACAAAGGCT TCTGATGTAG ACACCAGCTC CAGTACACTG 
AGGAAGATCC TCATGGACCC CAAATATGTG TCTGCCACAG GTGTTACTTC CACAAGTGTC 
ACTGCGGCCA TTGCAGAGCC TGTGAGTGCC CCTCGTCTGC AGGAGGCGCC GGCCCCTCCC 
TGTGAACCTA AGCGCCCTTC CTCAGAAGAG AAGGTAGCAG CCGCAGTACC ACATGCCGAC 
ACACAGGCCT CAGAGGTTCC AGTCGCTGCT GACAAAGAGA AGGTGGCCCC TGTCATCGCC 
CCTAAAATCA CATCAGTTAT TAGCCGGATG CCTGTTAGCA TCGACCTGGA GAACTCACAG 
AAGATAACGT TGGCAAAGCC GGCTCCGCAG ACCCTGACCG GCCTGGTGAG CGCCCTAACT 
GGCCTGGTGA ATGTCTCTCT GGTCCCAGTG AATGCCTTAA AGGGGCCTGT GAAGGGCTCT 
GTAGCCACCC TGAAAGGTTT GGTGAGCACC CCTGCTGGGC CTGTGAATCT CCTAAAGGGG 
CCCGTGAATG TCCTCACCGG TCCTGTCAAC GTCCTGACCA CTCCAGTGAG TGCCACGGTG 
GGCACAGTGA GCGCTGCCCC TGGTACCGTG ACTGCTGCCT GTGGTGTGAC TGCCACTACA 
GGCACAGCAG CCATGTCGGG TGCAGTGCCT GCCCCAGCAG CAAAGGGCAA GCAGAGGTCA 
AGCAGCAACG AGAACAGCCG CTTCCACCCT GGGTCAATGT CCGTCATCGA TGACCGCCCA 
GCAGACACAG GCTCTGGCGC AGGGCTGCGT GTGAATACTT CAGAAGGGGT CGTGCTCCTG 
AGCTACTCAG GACAGAAGAC GGAAGGCCCT CAGCGCATCA GTGCTAAGAT CAGCCAGATC 
CCTCCGGCCA GTGCAATGGA CATCGAGTTC CAGCAGTCAG TATCGAAGTC CCAGGTGAAA 
GCCGACTCCG TCACCCCGAC CCAGGCTGCA CCCAAAGGCC CTCAGACCCC TTCTGCCTTT 
GCAAACGTGG CTGCCCACTC TACCCTGGTC CTGACTGCCC AGACATACAA TGCCTCTCCC 
GTGATCTCCT CTGTGAAGAC CGACCGTCCA TCCTTGGAGA AGCCCGAGCC CATTCACCTC 
TCCGTGTCCA CCCCTGTCAC CCAGGGGGGC ACAGTGAAGG TTCTCACCCA GGGCATCAAC 
ACACCCCCAG TGCTGGTTCA CAACCAGCTT GTCCTCACCC CAAGCATCGT CACCACTAAC 
AAGAAACTTG CTGACCCTGT CACTCTGAAA ATAGAGACCA AGGTCCTTCA GCCGGCTAAC 
CTGGGGCCCA CCCTCACTCC CCACCACCCC CCTGCCCTTC CCAGCAAACT GCCGGCCGAA 
GTGAACCATG TTCCTTCCGG ACCCAGCAAC CCAGCAGATC GAACCATTGC CCACTTGGCA 
ACCACAAAAC CAGATACTCA TTCTCCTCGA CCCACTGGGC CTACACCAGG CCCATTCCCA 
AGGGCCTGCC ATCCCAGCAG CACGACATCC ACAGCGCTTT CCACCAATGC AACTGTCATG 
CTGGCTGCAG GCATTCCAGT GCCACAGTTC ATCTCCAGCA TACACCCAGA GCAGTCTGTC 
ATCATGCCCC CCCACAGCAT CACTCAGACC GTGTCTCTCG GTCACCTGTC CCAAGGCGAG 
GTGAGAATGA GCACACCCAC CTTGCCCAGC ATCACCTACA GCATCCGGCC AGAGACCCTG 
CACTCTCCTC GGGCCCCTCT GCAGCCCCAA CAGATAGAAG CAAGGGCCCC ACAGCGTGTG 
AGCACGCCCC AGCCAGCCAC AACTGCTGTG CCCGCGCTGG CCACACAGCA CCCTCCCGAG 
GAAGAAGTTC ACTACCACCT CCCTGTGGCT CGAGCTGCAG CCCCTGTGCA GTCAGAAGTG 
CTGGTCATGC AGTCCGAGTA CCGGTTACAC CCATACACCG TGCCCCGAGA CGTCCGGATC 
ATGGTGCATC CACATGTGAC TGCTGTCAGT GAACAGCCCA GGGCCACAGA GGGCGTGGTG 
AAGGTGCCAC CAGCCAGCAA GGCCCCCCAG CAACTGGTGA AAGAAGTGGT TAAGACTTCA 
GATGCCAAAG CAGTCCCAGC TCCAGCCCCC GTCCCGGTCC CTGTCCCTGT CCCGACCCCT 
GCCCCACCCC CTCATGGTGA GGCCCGAATT CTCACAGTCA CCCCCAGCAG CCAGCTTCAG 
GGGCTGCCTC TGACCCCGCC TGTGGTGGTA ACCCACGGGG TGCAGATTGT GCACTCCAGC 
GGGGAGCTGT TCCAAGAGTA TAGGTATGGC GACATCCGTA CCTACCACGC CCCTGCTCAG 
CTCACACATA CTCAGTTTCC TGTCGCCTCT TCCATCAACC TACCCTCCAG GACCAAGACC 
TCGGCCCAGG GCCCCCCCGA AAGTGAGCCC TTGCAGTCTA CGCAGCCTGC ACAGCCTGCC 
CCATCTACGC AGTCCACACA GCCTGTACCA CCTGCCCCAC CCTGCCAGCC CTCCCAGCTC 
AGCCAGCCTG CCCAGCCACC AAGTGGCAAG ATTCCTCAGG TCTCCCAAGA AGCCAAGGGG 
ACACAGACAG GAGGGATAGA GCAGACTCGC CTCCCAGCCA TGCCCACGAA CAGGCCGTCT 
GAGCCACATG CTCAGGCTCA GAGGGCACCG GGGGAAACCC CCCAACCAGC CCATCCCTCC 
CCCGTGTCAG TCGCCATGAA GCCGGACCTC CCAACCCCTC TTGCCTCTCA GGCTGCCCCA 
AAGCAGCCAT TGTTTGTCCC CGTAAACTCA GGCACGAGCA CCCCACCCGG ACTGGCTCTG 
CCTCATGCTG AAGCCCAGCC AGCCCCAAAG CAAGACTCTG CTCCACACGG GACACCACAA 
AGGCCCGTGG ATATGGTCCA GCTTCTGAAG AAGTACCCCA TCGTGTGGCA GGGCCTGCTG 
GCCCTCAAGA ACGACACAGC TGCTGTGCAG CTCCACTTTG TCTCTGGCAA CAACGTCCTG 
GCTCACCGCT CCCTGCCCCT CTCTGAAGGC GGCCCCCCGC TGCGGATCGC CCAAAGGATG 
CGACTGGAGG CCTCGCAACT GGAAGGAGTT GCCAGGAGGA TGACAGTGGA AACAGACTAC 
TGCCTGTTGC TGGCTCTGCC CTGTGGCCGT GACCAGGAGG ATGTCGTAAG CCAGACCGAG 
TCCCTCAAGG CTGCCTTTAT CACTTACCTG CAGGCCAAGC AAGCAGCAGG GATTATCAAT 
GTCCCCAATC CTGGCTCCAA TCAGCCCGCC TATGTGCTGC AGATCTTCCC ACCCTGTGAG 
TTCTCCGAGA GCCACCTCTC CCGGCTGGCC CCTGACCTCC TTGCCAGCAT CTCCAACATC 
TCTCCCCACC TCATGATTGT CATTGCCTCA GTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Insights into RNA biology from an atlas of mammalian mRNA-binding proteins.
Cell149(6)1393-406(2012 Jun)
Castello A,Fischer B,Eichelbaum K,Horos R,Beckmann BM,Strein C,Davey NE,Humphreys DT,Preiss T,Steinmetz LM,Krijgsveld J,Hentze MW

The mRNA-bound proteome and its global occupancy profile on protein-coding transcripts.
Molecular cell46(5)674-90(2012 Jun)
Baltz AG,Munschauer M,Schwanh?usser B,Vasile A,Murakawa Y,Schueler M,Youngs N,Penfold-Brown D,Drew K,Milek M,Wyler E,Bonneau R,Selbach M,Dieterich C,Landthaler M

Large-scale proteomics and phosphoproteomics of urinary exosomes.
Journal of the American Society of Nephrology : JASN20(2)363-79(2009 Feb)
Gonzales PA,Pisitkun T,Hoffert JD,Tchapyjnikov D,Star RA,Kleta R,Wang NS,Knepper MA

RBP-Jkappa/SHARP recruits CtIP/CtBP corepressors to silence Notch target genes.
Molecular and cellular biology25(23)10379-90(2005 Dec)
Oswald F,Winkler M,Cao Y,Astrahantseff K,Bourteele S,Kn?chel W,Borggrefe T

Interaction of the Epstein-Barr virus mRNA export factor EB2 with human Spen proteins SHARP, OTT1, and a novel member of the family, OTT3, links Spen proteins with splicing regulation and mRNA export.
The Journal of biological chemistry280(44)36935-45(2005 Nov)
Hiriart E,Gruffat H,Buisson M,Mikaelian I,Keppler S,Meresse P,Mercher T,Bernard OA,Sergeant A,Manet E

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Spen cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

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gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

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