SPEN cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	SPEN
Entrez Gene ID	23013
Full Name	spen family transcriptional repressor
Synonyms	HIAA0929,MINT,RBM15C,SHARP
General protein information	Preferred Names spen family transcriptional repressor Names msx2-interacting protein Msx2 interacting nuclear target (MINT) homolog SMART/HDAC1-associated repressor protein nuclear receptor transcription cofactor spen homolog, transcriptional regulator
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 1 Map Location: 1p36.21-p36.13
Summary	This gene encodes a hormone inducible transcriptional repressor. Repression of transcription by this gene product can occur through interactions with other repressors, by the recruitment of proteins involved in histone deacetylation, or through sequestration of transcriptional activators. The product of this gene contains a carboxy-terminal domain that permits binding to other corepressor proteins. This domain also permits interaction with members of the NuRD complex, a nucleosome remodeling protein complex that contains deacetylase activity. In addition, this repressor contains several RNA recognition motifs that confer binding to a steroid receptor RNA coactivator; this binding can modulate the activity of both liganded and nonliganded steroid receptors. [provided by RefSeq, Jul 2008].
Disorder MIM:	613484

mRNA and Protein(s)

mRNA	Protein	Name
NM_015001.2	NP_055816.2	msx2-interacting protein
NM_015001.3	NP_055816.2	msx2-interacting protein

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	SPEN	XP_003949352.1
	LOC696205	XP_002808320.1
Canis lupus familiaris (dog)	SPEN	XP_005618070.1
Bos taurus (cattle)	SPEN	XP_002694185.1
Rattus norvegicus (Norway rat)	LOC690911	NP_001258424.1
Mus musculus (house mouse)	Spen	NP_062737.2
Homo sapiens (human)	SPEN	NP_055816.2

Gene Ontology
Bibliography

Related articles in PubMed

SPEN, a new player in primary cilia formation and cell migration in breast cancer.
L?gar? S, Chabot C, Basik M
Breast cancer research : BCR19(1)104(2017 Sep)

The Estrogen Receptor Cofactor SPEN Functions as a Tumor Suppressor and Candidate Biomarker of Drug Responsiveness in Hormone-Dependent Breast Cancers.
L?gar? S, Cavallone L, Mamo A, Chabot C, Sirois I, Magliocco A, Klimowicz A, Tonin PN, Buchanan M, Keilty D, Hassan S, Laperri?re D, Mader S, Aleynikova O, Basik M
Cancer research75(20)4351-63(2015 Oct)

The NOTCH pathway is recurrently mutated in diffuse large B-cell lymphoma associated with hepatitis C virus infection.
Arcaini L, Rossi D, Lucioni M, Nicola M, Bruscaggin A, Fiaccadori V, Riboni R, Ramponi A, Ferretti VV, Cresta S, Casaluci GM, Bonfichi M, Gotti M, Merli M, Maffi A, Arra M, Varettoni M, Rattotti S, Morello L, Guerrera ML, Sciarra R, Gaidano G, Cazzola M, Paulli M
Haematologica100(2)246-52(2015 Feb)

Proteomic analyses reveal distinct chromatin-associated and soluble transcription factor complexes.
Li X, Wang W, Wang J, Malovannaya A, Xi Y, Li W, Guerra R, Hawke DH, Qin J, Chen J
Molecular systems biology11(1)775(2015 Jan)

The crystal structure of the Split End protein SHARP adds a new layer of complexity to proteins containing RNA recognition motifs.
Arieti F, Gabus C, Tambalo M, Huet T, Round A, Thore S
Nucleic acids research42(10)6742-52(2014 Jun)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

SPEN is a novel tumor-suppressor gene that may be clinically useful as a predictive biomarker of tamoxifen response in ERalpha-positive breast cancers.
Title: The Estrogen Receptor Cofactor SPEN Functions as a Tumor Suppressor and Candidate Biomarker of Drug Responsiveness in Hormone-Dependent Breast Cancers.

SPEN mutations were associated with diffuse large B-cell lymphoma with hepatitis C virus infection.
Title: The NOTCH pathway is recurrently mutated in diffuse large B-cell lymphoma associated with hepatitis C virus infection.

A structural study of the RNA binding properties of the RNA recognition motifs of SHARP.
Title: The crystal structure of the Split End protein SHARP adds a new layer of complexity to proteins containing RNA recognition motifs.

Phosphorylation of the CK2 site on SMRT significantly increased affinity for SHARP.
Title: Structural insights into the recruitment of SMRT by the corepressor SHARP under phosphorylative regulation.

These results demonstrate a role for the inactivation of SHARP transcription in DM1 biology.
Title: RNA steady-state defects in myotonic dystrophy are linked to nuclear exclusion of SHARP.

The following SPEN gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the SPEN cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu13146	NM_015001.3 Latest version!	Homo sapiens spen family transcriptional repressor (SPEN), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
View Old Accession Versions >>
OHu13146	NM_015001.2	Homo sapiens spen family transcriptional repressor (SPEN), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

SPEN ( NM_015001.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_055816.2 Homo sapiens spen family transcriptional repressor (SPEN), mRNA.

Price & Availability↑

CloneID	OHu13146
Clone ID Related Accession (Same CDS sequence)	NM_015001.2 , NM_015001.3
Accession Version	NM_015001.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 10995bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-30
Organism	Homo sapiens(human)
Product	msx2-interacting protein
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AF356524.1. This sequence is a reference standard in the RefSeqGene project. On Jul 17, 2001 this sequence version replaced NM_015001.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF356524.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: full length.

ATGGTCCGGG AAACCAGGCA TCTCTGGGTG GGCAACTTAC CCGAGAACGT GCGGGAAGAG 
AAGATCATCG AGCATTTCAA ACGATATGGC CGCGTGGAAA GTGTCAAAAT TCTTCCCAAG 
AGGGGATCTG AAGGAGGAGT GGCTGCCTTT GTGGATTTTG TGGACATCAA AAGTGCACAG 
AAAGCTCACA ACTCGGTCAA CAAAATGGGT GACAGAGACC TACGCACGGA TTATAATGAA 
CCAGGCACCA TCCCGAGTGC TGCTCGGGGA TTGGATGATA CAGTTTCCAT AGCATCTCGT 
AGTAGAGAGG TTTCTGGGTT CAGAGGAGGT GGTGGAGGGC CTGCTTATGG TCCCCCACCG 
TCACTTCATG CACGAGAAGG ACGTTATGAG CGGAGACTTG ATGGGGCTTC AGATAACAGG 
GAGCGTGCTT ATGAACATAG TGCCTATGGA CACCATGAAC GGGGGACGGG AGGATTTGAT 
CGGACAAGAC ATTACGATCA GGATTACTAT AGAGATCCTC GAGAGCGGAC TTTACAACAT 
GGGCTCTATT ACGCTTCTCG GAGTCGAAGT CCAAATCGCT TTGATGCTCA TGACCCCCGA 
TATGAACCTA GGGCTCGCGA GCAGTTTACA CTGCCCAGTG TGGTACACAG GGATATCTAC 
AGGGATGATA TTACCCGGGA GGTACGAGGC AGAAGGCCAG AGCGGAATTA CCAGCACAGC 
AGGAGTCGGT CACCACATTC ATCCCAGTCT AGAAATCAGT CTCCTCAGAG ACTGGCTAGC 
CAAGCATCTA GACCCACAAG GTCCCCTAGC GGCAGCGGCT CTAGAAGTAG ATCCTCCAGT 
AGTGATTCAA TCAGCAGCAG CAGTAGTACC AGCAGTGACA GCAGTGATTC CAGCAGTAGT 
TCAAGTGATG ATTCTCCAGC TCGATCAGTT CAGTCTGCAG CAGTCCCTGC ACCCACTTCC 
CAGTTGCTTT CATCTCTGGA AAAAGATGAG CCCCGTAAAA GTTTTGGCAT CAAGGTCCAG 
AATCTTCCAG TACGCTCTAC AGATACAAGC CTTAAAGATG GCCTTTTCCA TGAATTTAAG 
AAATTTGGAA AAGTAACTTC AGTGCAGATA CATGGAACTT CAGAAGAGAG GTATGGTCTG 
GTATTCTTTC GGCAGCAAGA GGACCAAGAA AAAGCCTTGA CTGCATCAAA AGGAAAACTT 
TTCTTTGGCA TGCAGATTGA AGTAACAGCA TGGATAGGTC CAGAAACAGA AAGTGAAAAT 
GAATTTCGCC CCTTGGATGA AAGGATAGAT GAATTTCACC CCAAAGCAAC AAGAACTCTC 
TTTATTGGCA ACCTTGAAAA AACCACTACT TACCATGACC TTCGCAACAT CTTCCAGCGC 
TTTGGAGAAA TTGTGGATAT TGACATTAAG AAAGTAAATG GAGTTCCTCA GTATGCGTTT 
CTGCAATACT GTGATATTGC TAGCGTTTGT AAAGCTATTA AGAAGATGGA TGGGGAATAT 
CTTGGAAATA ATCGCCTCAA GCTGGGTTTT GGAAAGAGCA TGCCTACAAA CTGCGTGTGG 
CTAGATGGGC TTTCTTCGAA TGTGTCAGAT CAGTATTTAA CACGACATTT CTGCCGATAT 
GGGCCTGTGG TAAAGGTGGT GTTTGACCGC TTAAAAGGCA TGGCCCTGGT TCTCTACAAT 
GAAATTGAAT ATGCACAAGC AGCTGTAAAA GAGACCAAAG GGAGGAAAAT CGGTGGGAAT 
AAAATTAAGG TGGATTTTGC AAATCGGGAA AGTCAGCTGG CTTTTTATCA CTGCATGGAG 
AAATCTGGTC AAGACATCAG AGACTTTTAT GAAATGTTAG CCGAAAGAAG AGAGGAACGA 
AGGGCATCCT ACGACTATAA CCAAGATCGT ACATATTATG AGAGTGTTCG AACTCCAGGC 
ACTTATCCTG AGGATTCCAG GCGGGACTAT CCAGCTCGAG GGAGAGAGTT TTATTCAGAA 
TGGGAAACTT ACCAAGGAGA CTACTATGAA TCACGATACT ACGATGATCC TCGGGAATAC 
AGGGATTACA GGAATGATCC TTATGAACAA GATATTAGGG AATATAGTTA CAGGCAAAGG 
GAACGAGAAA GAGAACGTGA AAGATTTGAG TCTGACCGGG ACAGAGACCA TGAGAGGAGG 
CCGATTGAAC GAAGTCAAAG TCCTGTTCAC TTGCGACGTC CACAGAGTCC TGGAGCGTCT 
CCCTCTCAGG CAGAGAGGTT GCCGAGTGAT TCTGAGAGGA GGCTTTACAG CCGATCCTCA 
GACCGGAGTG GAAGCTGTAG CTCACTCTCC CCTCCAAGAT ATGAGAAACT GGACAAGTCT 
CGTTTGGAGC GCTATACAAA AAATGAAAAG ACAGATAAAG AACGAACTTT TGATCCGGAG 
AGAGTGGAGA GAGAGAGACG CTTAATACGG AAGGAAAAAG TGGAAAAGGA CAAAACTGAC 
AAGCAGAAAC GCAAAGGAAA GGTTCACTCC CCTAGTTCTC AGTCTTCAGA AACGGACCAA 
GAAAATGAGC GAGAGCAAAG CCCTGAAAAG CCCAGGAGTT GTAATAAACT GAGCAGAGAG 
AAAGCTGACA AAGAGGGAAT AGCGAAAAAC CGCCTGGAAC TCATGCCTTG CGTGGTTTTG 
ACTCGAGTGA AAGAGAAAGA GGGAAAGGTC ATTGACCACA CTCCTGTGGA AAAGTTGAAA 
GCCAAGCTTG ATAATGACAC TGTCAAATCT TCTGCCCTGG ACCAGAAACT TCAGGTCTCT 
CAGACGGAGC CTGCAAAATC TGACTTGTCT AAACTGGAAT CAGTTAGAAT GAAAGTACCA 
AAGGAAAAGG GGCTTTCAAG CCATGTTGAA GTGGTGGAGA AGGAAGGCAG GCTTAAAGCC 
AGGAAGCACC TCAAGCCTGA GCAGCCTGCA GATGGGGTAA GTGCTGTGGA TCTGGAGAAG 
CTGGAAGCCA GGAAAAGGCG CTTTGCAGAT TCCAATTTAA AAGCAGAAAA GCAAAAACCA 
GAGGTCAAGA AAAGCAGTCC AGAGATGGAG GATGCTCGCG TGCTTTCAAA AAAGCAGCCT 
GACGTGTCCT CTAGAGAGGT CATTCTGCTG AGGGAAGGAG AGGCTGAAAG AAAGCCTGTG 
AGGAAAGAAA TTCTTAAAAG AGAATCTAAA AAAATCAAAC TGGACAGACT TAATACTGTT 
GCCAGCCCCA AAGACTGTCA GGAGCTTGCC AGTATTTCTG TTGGGTCTGG CTCAAGGCCC 
AGCTCAGACC TACAAGCAAG ACTGGGAGAA CTAGCAGGTG AATCTGTGGA AAATCAAGAA 
GTCCAATCAA AAAAGCCCAT TCCCTCAAAA CCACAGCTCA AACAGCTGCA GGTATTAGAT 
GATCAAGGAC CAGAGAGAGA AGACGTTAGG AAAAACTATT GCAGTCTTCG TGATGAAACA 
CCTGAACGTA AATCAGGCCA AGAGAAATCA CATTCAGTAA ATACTGAAGA AAAAATTGGC 
ATTGACATCG ATCACACGCA GAGTTACCGA AAACAAATGG AACAGAGTCG TAGGAAACAG 
CAGATGGAAA TGGAAATAGC CAAGTCTGAG AAGTTTGGCA GTCCTAAAAA AGATGTAGAT 
GAATATGAAA GACGTAGCCT CGTTCACGAG GTAGGCAAAC CCCCTCAAGA TGTCACTGAT 
GACTCTCCTC CTAGCAAAAA GAAAAGGATG GATCATGTCG ATTTTGATAT CTGCACCAAG 
CGAGAACGGA ATTACAGAAG TTCACGCCAA ATCAGCGAAG ATTCTGAAAG GACTGGTGGT 
TCTCCCAGTG TCCGACATGG TTCCTTCCAT GAAGATGAGG ATCCCATAGG CTCCCCTAGG 
CTACTGTCAG TAAAAGGGTC TCCTAAAGTA GATGAAAAAG TCCTCCCCTA TTCTAACATA 
ACAGTCAGGG AAGAGTCTTT AAAATTTAAT CCTTATGATT CTAGCAGGAG AGAACAGATG 
GCAGATATGG CCAAAATAAA ACTATCTGTC TTGAATTCTG AAGATGAACT AAATCGTTGG 
GACTCTCAGA TGAAACAGGA TGCTGGCAGA TTTGATGTGA GTTTCCCAAA CAGCATAATT 
AAGAGAGATA GCCTTCGAAA AAGGTCTGTA CGAGATCTGG AACCTGGTGA GGTGCCTTCT 
GATTCTGACG AAGATGGTGA ACACAAATCC CACTCACCCA GAGCCTCTGC ATTATATGAA 
AGTTCTCGAT TGTCTTTTTT ATTGAGGGAC AGAGAAGACA AGCTACGTGA GCGAGATGAA 
AGACTCTCTA GTTCTTTAGA AAGGAACAAA TTTTACTCTT TTGCATTGGA TAAGACAATC 
ACACCAGACA CTAAAGCTTT GCTTGAAAGA GCTAAATCCC TCTCTTCATC TCGTGAAGAA 
AATTGGTCTT TTCTTGATTG GGACTCCCGA TTTGCAAATT TTCGAAACAA CAAAGATAAA 
GAAAAGGTTG ACTCTGCTCC AAGACCTATT CCATCCTGGT ACATGAAAAA GAAGAAAATT 
AGGACTGATT CAGAAGGGAA AATGGATGAT AAGAAAGAGG ACCATAAAGA AGAAGAGCAA 
GAGAGGCAGG AATTGTTTGC TTCTCGTTTT TTACACAGCT CAATCTTTGA ACAAGATTCC 
AAGCGATTGC AGCATCTAGA GAGAAAAGAG GAAGATTCTG ACTTCATTTC TGGTAGGATC 
TATGGGAAGC AGACATCTGA GGGAGCAAAC AGCACAACTG ATTCCATTCA AGAACCAGTA 
GTTCTGTTCC ATAGCAGATT TATGGAGCTC ACACGGATGC AACAGAAAGA AAAAGAAAAA 
GACCAGAAAC CCAAAGAGGT TGAGAAACAG GAAGATACAG AGAATCATCC CAAGACCCCA 
GAATCTGCTC CTGAGAATAA AGATTCAGAA CTGAAAACTC CACCTTCCGT TGGGCCTCCA 
AGTGTCACAG TCGTAACTCT AGAATCAGCC CCATCAGCAC TAGAGAAGAC CACTGGTGAC 
AAAACGGTAG AGGCGCCTTT GGTAACAGAA GAGAAGACTG TGGAGCCAGC TACCGTCTCA 
GAAGAAGCAA AGCCTGCATC TGAACCTGCT CCTGCCCCTG TGGAACAGCT GGAACAAGTA 
GACCTGCCCC CAGGAGCAGA CCCCGATAAA GAAGCTGCCA TGATGCCTGC GGGTGTTGAG 
GAAGGTTCAT CAGGTGACCA GCCGCCTTAT CTGGATGCCA AGCCTCCAAC TCCCGGGGCC 
TCGTTTTCCC AGGCAGAGAG CAACGTAGAT CCAGAGCCTG ACAGTACCCA GCCACTTTCA 
AAACCAGCTC AGAAGTCTGA GGAAGCCAAT GAGCCAAAGG CCGAAAAGCC AGACGCCACT 
GCAGATGCTG AGCCTGATGC AAACCAGAAA GCCGAAGCTG CTCCTGAGTC TCAGCCCCCA 
GCTTCTGAAG ATTTAGAGGT TGATCCTCCA GTTGCTGCAA AGGATAAAAA GCCAAACAAA 
AGCAAGCGTT CAAAGACCCC TGTTCAGGCA GCTGCAGTGA GTATCGTGGA GAAGCCCGTC 
ACAAGGAAGA GTGAGAGGAT AGACCGGGAA AAACTCAAGC GGTCCAATTC TCCTCGGGGA 
GAAGCACAGA AGCTTTTGGA ATTGAAGATG GAGGCAGAGA AGATTACAAG GACTGCTTCT 
AAAAACTCTG CTGCAGACCT TGAACATCCC GAACCAAGTT TGCCTCTCAG CCGAACAAGG 
CGCCGGAATG TAAGGAGCGT CTATGCAACC ATGGGTGACC ATGAAAACCG CTCTCCTGTC 
AAAGAGCCCG TTGAGCAACC AAGAGTGACC AGAAAGAGAT TGGAGCGAGA GCTTCAGGAG 
GCTGCAGCGG TTCCCACCAC CCCTCGGAGG GGAAGGCCTC CAAAGACACG CCGGCGAGCC 
GATGAAGAGG AGGAGAACGA GGCCAAGGAA CCTGCAGAAA CACTCAAGCC ACCTGAGGGA 
TGGCGGTCGC CAAGGTCCCA GAAAACTGCA GCTGGTGGTG GACCCCAAGG GAAAAAGGGA 
AAAAATGAAC CGAAGGTGGA TGCTACACGT CCTGAGGCCA CCACTGAGGT GGGCCCCCAA 
ATAGGCGTGA AAGAGAGCTC CATGGAACCC AAGGCTGCTG AGGAGGAGGC AGGGAGTGAA 
CAGAAACGTG ACAGAAAAGA TGCTGGCACA GACAAAAACC CCCCTGAAAC CGCCCCTGTT 
GAAGTTGTAG AGAAAAAACC GGCCCCTGAA AAAAACTCCA AATCAAAGAG AGGAAGATCT 
CGAAACTCCA GGTTAGCAGT GGACAAATCT GCAAGTCTGA AAAATGTGGA TGCTGCTGTC 
AGTCCCAGGG GGGCTGCAGC ACAGGCAGGG GAGAGGGAAT CTGGGGTGGT GGCAGTCTCC 
CCTGAGAAAA GTGAGAGTCC CCAAAAGGAG GATGGTTTAT CATCCCAGTT GAAAAGTGAT 
CCAGTTGATC CAGACAAGGA ACCAGAGAAA GAAGACGTGT CTGCCTCTGG GCCGTCCCCA 
GAAGCCACCC AGTTAGCCAA GCAGATGGAG CTGGAGCAGG CCGTGGAACA CATCGCAAAG 
CTCGCTGAGG CCTCTGCCTC TGCTGCCTAT AAGGCAGATG CACCAGAGGG CCTTGCCCCA 
GAGGACAGGG ACAAGCCTGC ACACCAAGCA AGTGAAACAG AGCTGGCTGC GGCCATCGGC 
TCCATCATCA ATGACATTTC TGGGGAGCCA GAAAACTTCC CAGCACCTCC ACCTTATCCT 
GGAGAATCCC AGACAGATCT GCAACCCCCC GCAGGTGCAC AGGCGCTGCA GCCTTCTGAG 
GAAGGAATGG AGACAGATGA GGCTGTATCT GGCATCCTGG AAACTGAGGC TGCTACAGAA 
TCTTCTAGGC CTCCAGTCAA TGCTCCTGAC CCCTCAGCCG GCCCAACAGA TACCAAGGAA 
GCCAGAGGAA ATAGCAGTGA AACCTCACAC TCAGTGCCAG AAGCCAAAGG GTCTAAAGAA 
GTGGAAGTCA CTCTTGTTCG GAAAGACAAA GGGCGCCAGA AAACAACCCG ATCACGCCGC 
AAGCGAAACA CAAACAAGAA AGTGGTGGCT CCTGTAGAGA GCCATGTCCC TGAATCCAAC 
CAAGCTCAAG GTGAGAGTCC TGCTGCAAAT GAGGGGACAA CAGTACAGCA CCCCGAAGCC 
CCACAGGAAG AAAAGCAGAG TGAGAAACCC CATTCCACTC CTCCTCAGTC ATGTACTTCT 
GACCTAAGCA AGATTCCCTC CACAGAGAAT TCGTCCCAAG AAATCAGTGT TGAGGAAAGG 
ACTCCAACCA AAGCATCTGT GCCCCCAGAC CTTCCCCCAC CTCCCCAGCC AGCACCGGTG 
GATGAGGAGC CTCAAGCCAG GTTCAGGGTG CATTCCATCA TTGAAAGTGA CCCGGTGACC 
CCACCCAGCG ATCCAAGCAT CCCCATACCC ACACTGCCTT CTGTAACTGC AGCAAAGCTC 
TCACCTCCTG TCGCCTCTGG GGGGATCCCA CACCAGAGCC CCCCTACTAA GGTGACAGAG 
TGGATCACAA GGCAGGAGGA GCCACGGGCT CAGTCTACTC CATCTCCAGC TCTTCCCCCA 
GACACAAAGG CCTCTGATGT TGACACCAGC TCCAGCACCC TGAGGAAGAT TCTCATGGAC 
CCCAAGTATG TGTCTGCCAC AAGTGTCACT TCCACAAGTG TCACCACAGC CATTGCAGAG 
CCTGTCAGTG CTGCCCCTTG CCTACATGAG GCCCCGCCCC CGCCAGTTGA CTCTAAAAAG 
CCTTTAGAAG AAAAAACAGC ACCTCCAGTG ACAAACAACT CTGAGATACA AGCCTCGGAG 
GTGCTGGTAG CTGCTGACAA GGAAAAGGTG GCTCCAGTCA TTGCTCCCAA AATTACCTCT 
GTTATTAGCC GGATGCCTGT CAGCATTGAC CTGGAAAATT CACAGAAGAT AACCTTGGCA 
AAACCAGCTC CTCAAACCCT CACTGGTCTG GTGAGCGCAC TCACTGGCCT GGTGAACGTC 
TCCCTGGTCC CGGTGAATGC CCTGAAAGGC CCCGTGAAGG GCTCAGTGAC CACACTGAAA 
AGTTTGGTGA GCACCCCTGC TGGGCCCGTG AACGTCCTGA AAGGGCCTGT GAATGTTCTT 
ACGGGGCCAG TGAATGTTCT CACCACTCCA GTGAACGCCA CGGTGGGCAC AGTGAATGCC 
GCCCCAGGCA CAGTCAATGC CGCTGCGAGT GCAGTGAATG CCACAGCAAG TGCAGTGACC 
GTCACAGCGG GTGCGGTTAC TGCTGCATCT GGTGGTGTAA CGGCCACAAC AGGCACGGTG 
ACAATGGCAG GGGCAGTGAT TGCGCCGTCA ACAAAGTGCA AACAGAGAGC GAGTGCTAAT 
GAAAACAGTC GGTTCCACCC AGGGTCCATG CCTGTGATCG ACGATCGTCC GGCAGACGCG 
GGCTCAGGGG CGGGGCTGCG TGTGAACACT TCTGAAGGGG TTGTGCTCCT GAGTTACTCA 
GGGCAGAAGA CCGAAGGCCC ACAGCGGATC AGCGCCAAGA TCAGCCAGAT CCCCCCGGCC 
AGTGCAATGG ACATTGAATT TCAGCAGTCA GTGTCCAAGT CCCAGGTCAA ACCTGATTCT 
GTCACAGCAT CGCAGCCTCC ATCCAAAGGC CCTCAAGCTC CTGCAGGCTA TGCGAACGTG 
GCCACCCATT CCACGTTGGT ACTGACCGCC CAGACATATA ATGCCTCTCC TGTGATTTCG 
TCTGTGAAGG CCGATAGGCC ATCCTTGGAG AAGCCCGAGC CCATTCACCT CTCGGTGTCC 
ACGCCTGTCA CCCAGGGAGG CACAGTGAAG GTTCTCACCC AGGGGATCAA CACACCCCCT 
GTGCTGGTTC ACAACCAGCT GGTCCTCACC CCAAGCATTG TCACCACAAA CAAGAAGCTT 
GCTGACCCCG TCACCCTTAA AATCGAGACC AAGGTCCTTC AGCCGGCCAA CCTGGGGTCC 
ACGCTCACGC CCCACCACCC TCCTGCTCTG CCCAGCAAAC TGCCTACAGA AGTCAACCAT 
GTCCCCTCGG GGCCCAGCAT CCCAGCAGAT CGAACTGTCT CCCATTTGGC AGCTGCAAAG 
CTAGATGCTC ATTCTCCTCG ACCAAGTGGA CCCGGGCCAT CCTCATTCCC AAGGGCAAGC 
CACCCCAGCA GTACTGCATC TACGGCGCTC TCCACCAACG CCACAGTCAT GCTGGCTGCA 
GGCATCCCAG TGCCCCAGTT CATCTCCAGC ATCCACCCAG AGCAGTCTGT CATCATGCCA 
CCCCACAGCA TCACCCAGAC TGTGTCCCTG AGCCACCTCT CCCAGGGCGA GGTGAGAATG 
AACACTCCCA CGCTGCCCAG TATCACCTAC AGCATCCGGC CAGAAGCGCT TCACTCTCCT 
CGGGCTCCGC TGCAGCCCCA GCAAATAGAG GTCAGGGCCC CACAGCGTGC CAGCACCCCG 
CAGCCAGCCC CAGCTGGTGT GCCTGCACTG GCCTCCCAGC ACCCTCCCGA GGAGGAAGTG 
CATTATCACC TTCCTGTCGC TCGAGCCACA GCCCCTGTGC AGTCAGAGGT ACTAGTCATG 
CAGTCTGAGT ACCGACTGCA CCCCTATACT GTGCCACGGG ATGTGAGGAT CATGGTGCAT 
CCACATGTGA CGGCAGTCAG CGAGCAGCCC AGGGCCGCGG ATGGGGTGGT GAAGGTGCCA 
CCAGCCAGCA AGGCCCCTCA GCAGCCAGGG AAGGAAGCTG CCAAGACACC AGATGCCAAA 
GCTGCCCCCA CCCCCACCCC TGCCCCCGTC CCTGTCCCTG TCCCCCTTCC TGCCCCTGCT 
CCTGCCCCTC ATGGTGAGGC CCGTATCCTC ACAGTTACCC CCAGTAACCA ACTCCAGGGG 
CTGCCTCTGA CCCCTCCTGT GGTGGTGACC CATGGGGTGC AGATTGTGCA CTCCAGCGGG 
GAGCTGTTTC AAGAGTACCG GTACGGCGAC ATCCGCACCT ACCACCCCCC GGCCCAGCTC 
ACACACACTC AGTTTCCCGC CGCTTCCTCT GTTGGCCTGC CTTCCCGGAC CAAGACAGCT 
GCTCAGGGCC CTCCTCCTGA AGGTGAGCCC CTGCAGCCTC CTCAGCCTGT GCAGTCCACA 
CAGCCTGCCC AGCCTGCACC ACCCTGCCCG CCCTCCCAGC TCGGTCAGCC CGGCCAGCCA 
CCAAGCAGCA AGATGCCTCA AGTGTCCCAG GAGGCAAAGG GGACCCAGAC GGGAGTAGAG 
CAGCCTCGCC TCCCAGCTGG ACCTGCAAAC AGGCCACCTG AGCCTCACAC CCAGGTTCAG 
AGGGCACAAG CAGAAACAGG CCCGACTTCC TTCCCCTCCC CTGTGTCTGT CTCCATGAAG 
CCTGACCTTC CAGTCTCTCT TCCCACTCAG ACTGCCCCAA AACAGCCGTT GTTTGTCCCA 
ACAACCTCTG GCCCCAGCAC CCCACCAGGA CTGGTTCTGC CACACACTGA ATTCCAGCCA 
GCCCCCAAAC AAGATTCCTC TCCACACCTG ACTTCCCAGA GACCCGTGGA TATGGTTCAA 
CTTCTGAAGA AGTACCCCAT CGTGTGGCAG GGCCTGCTGG CCCTCAAGAA TGACACAGCT 
GCTGTGCAGC TCCACTTCGT CTCTGGCAAC AACGTCCTGG CCCATCGGTC CCTGCCCCTT 
TCTGAAGGAG GGCCCCCACT AAGGATCGCC CAGAGGATGC GGCTGGAGGC AACGCAGCTG 
GAAGGGGTTG CCCGAAGGAT GACGGTGGAG ACAGATTACT GTCTGCTGCT GGCTCTGCCC 
TGTGGCCGTG ACCAAGAGGA TGTTGTGAGC CAGACCGAGT CCCTCAAGGC TGCCTTCATC 
ACTTACCTGC AGGCCAAGCA GGCGGCAGGG ATCATCAACG TTCCCAACCC TGGCTCCAAT 
CAGCCTGCCT ACGTGCTGCA GATCTTCCCG CCCTGTGAGT TCTCTGAGAG TCACCTGTCC 
CGCCTGGCCC CTGACCTCCT TGCCAGCATC TCCAACATCT CTCCCCACCT CATGATTGTC 
ATTGCCTCCG TGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_055816.2
CDS	205..11199
Translation	MVRETRHLWVGNLPENVREEKIIEHFKRYGRVESVKILPKRGSEGGVAAFVDFVDIKSAQKAHNSVNKMGDRDLRTDYNEPGTIPSAARGLDDTVSIASRSREVSGFRGGGGGPAYGPPPSLHAREGRYERRLDGASDNRERAYEHSAYGHHERGTGGFDRTRHYDQDYYRDPRERTLQHGLYYASRSRSPNRFDAHDPRYEPRAREQFTLPSVVHRDIYRDDITREVRGRRPERNYQHSRSRSPHSSQSRNQSPQRLASQASRPTRSPSGSGSRSRSSSSDSISSSSSTSSDSSDSSSSSSDDSPARSVQSAAVPAPTSQLLSSLEKDEPRKSFGIKVQNLPVRSTDTSLKDGLFHEFKKFGKVTSVQIHGTSEERYGLVFFRQQEDQEKALTASKGKLFFGMQIEVTAWIGPETESENEFRPLDERIDEFHPKATRTLFIGNLEKTTTYHDLRNIFQRFGEIVDIDIKKVNGVPQYAFLQYCDIASVCKAIKKMDGEYLGNNRLKLGFGKSMPTNCVWLDGLSSNVSDQYLTRHFCRYGPVVKVVFDRLKGMALVLYNEIEYAQAAVKETKGRKIGGNKIKVDFANRESQLAFYHCMEKSGQDIRDFYEMLAERREERRASYDYNQDRTYYESVRTPGTYPEDSRRDYPARGREFYSEWETYQGDYYESRYYDDPREYRDYRNDPYEQDIREYSYRQRERERERERFESDRDRDHERRPIERSQSPVHLRRPQSPGASPSQAERLPSDSERRLYSRSSDRSGSCSSLSPPRYEKLDKSRLERYTKNEKTDKERTFDPERVERERRLIRKEKVEKDKTDKQKRKGKVHSPSSQSSETDQENEREQSPEKPRSCNKLSREKADKEGIAKNRLELMPCVVLTRVKEKEGKVIDHTPVEKLKAKLDNDTVKSSALDQKLQVSQTEPAKSDLSKLESVRMKVPKEKGLSSHVEVVEKEGRLKARKHLKPEQPADGVSAVDLEKLEARKRRFADSNLKAEKQKPEVKKSSPEMEDARVLSKKQPDVSSREVILLREGEAERKPVRKEILKRESKKIKLDRLNTVASPKDCQELASISVGSGSRPSSDLQARLGELAGESVENQEVQSKKPIPSKPQLKQLQVLDDQGPEREDVRKNYCSLRDETPERKSGQEKSHSVNTEEKIGIDIDHTQSYRKQMEQSRRKQQMEMEIAKSEKFGSPKKDVDEYERRSLVHEVGKPPQDVTDDSPPSKKKRMDHVDFDICTKRERNYRSSRQISEDSERTGGSPSVRHGSFHEDEDPIGSPRLLSVKGSPKVDEKVLPYSNITVREESLKFNPYDSSRREQMADMAKIKLSVLNSEDELNRWDSQMKQDAGRFDVSFPNSIIKRDSLRKRSVRDLEPGEVPSDSDEDGEHKSHSPRASALYESSRLSFLLRDREDKLRERDERLSSSLERNKFYSFALDKTITPDTKALLERAKSLSSSREENWSFLDWDSRFANFRNNKDKEKVDSAPRPIPSWYMKKKKIRTDSEGKMDDKKEDHKEEEQERQELFASRFLHSSIFEQDSKRLQHLERKEEDSDFISGRIYGKQTSEGANSTTDSIQEPVVLFHSRFMELTRMQQKEKEKDQKPKEVEKQEDTENHPKTPESAPENKDSELKTPPSVGPPSVTVVTLESAPSALEKTTGDKTVEAPLVTEEKTVEPATVSEEAKPASEPAPAPVEQLEQVDLPPGADPDKEAAMMPAGVEEGSSGDQPPYLDAKPPTPGASFSQAESNVDPEPDSTQPLSKPAQKSEEANEPKAEKPDATADAEPDANQKAEAAPESQPPASEDLEVDPPVAAKDKKPNKSKRSKTPVQAAAVSIVEKPVTRKSERIDREKLKRSNSPRGEAQKLLELKMEAEKITRTASKNSAADLEHPEPSLPLSRTRRRNVRSVYATMGDHENRSPVKEPVEQPRVTRKRLERELQEAAAVPTTPRRGRPPKTRRRADEEEENEAKEPAETLKPPEGWRSPRSQKTAAGGGPQGKKGKNEPKVDATRPEATTEVGPQIGVKESSMEPKAAEEEAGSEQKRDRKDAGTDKNPPETAPVEVVEKKPAPEKNSKSKRGRSRNSRLAVDKSASLKNVDAAVSPRGAAAQAGERESGVVAVSPEKSESPQKEDGLSSQLKSDPVDPDKEPEKEDVSASGPSPEATQLAKQMELEQAVEHIAKLAEASASAAYKADAPEGLAPEDRDKPAHQASETELAAAIGSIINDISGEPENFPAPPPYPGESQTDLQPPAGAQALQPSEEGMETDEAVSGILETEAATESSRPPVNAPDPSAGPTDTKEARGNSSETSHSVPEAKGSKEVEVTLVRKDKGRQKTTRSRRKRNTNKKVVAPVESHVPESNQAQGESPAANEGTTVQHPEAPQEEKQSEKPHSTPPQSCTSDLSKIPSTENSSQEISVEERTPTKASVPPDLPPPPQPAPVDEEPQARFRVHSIIESDPVTPPSDPSIPIPTLPSVTAAKLSPPVASGGIPHQSPPTKVTEWITRQEEPRAQSTPSPALPPDTKASDVDTSSSTLRKILMDPKYVSATSVTSTSVTTAIAEPVSAAPCLHEAPPPPVDSKKPLEEKTAPPVTNNSEIQASEVLVAADKEKVAPVIAPKITSVISRMPVSIDLENSQKITLAKPAPQTLTGLVSALTGLVNVSLVPVNALKGPVKGSVTTLKSLVSTPAGPVNVLKGPVNVLTGPVNVLTTPVNATVGTVNAAPGTVNAAASAVNATASAVTVTAGAVTAASGGVTATTGTVTMAGAVIAPSTKCKQRASANENSRFHPGSMPVIDDRPADAGSGAGLRVNTSEGVVLLSYSGQKTEGPQRISAKISQIPPASAMDIEFQQSVSKSQVKPDSVTASQPPSKGPQAPAGYANVATHSTLVLTAQTYNASPVISSVKADRPSLEKPEPIHLSVSTPVTQGGTVKVLTQGINTPPVLVHNQLVLTPSIVTTNKKLADPVTLKIETKVLQPANLGSTLTPHHPPALPSKLPTEVNHVPSGPSIPADRTVSHLAAAKLDAHSPRPSGPGPSSFPRASHPSSTASTALSTNATVMLAAGIPVPQFISSIHPEQSVIMPPHSITQTVSLSHLSQGEVRMNTPTLPSITYSIRPEALHSPRAPLQPQQIEVRAPQRASTPQPAPAGVPALASQHPPEEEVHYHLPVARATAPVQSEVLVMQSEYRLHPYTVPRDVRIMVHPHVTAVSEQPRAADGVVKVPPASKAPQQPGKEAAKTPDAKAAPTPTPAPVPVPVPLPAPAPAPHGEARILTVTPSNQLQGLPLTPPVVVTHGVQIVHSSGELFQEYRYGDIRTYHPPAQLTHTQFPAASSVGLPSRTKTAAQGPPPEGEPLQPPQPVQSTQPAQPAPPCPPSQLGQPGQPPSSKMPQVSQEAKGTQTGVEQPRLPAGPANRPPEPHTQVQRAQAETGPTSFPSPVSVSMKPDLPVSLPTQTAPKQPLFVPTTSGPSTPPGLVLPHTEFQPAPKQDSSPHLTSQRPVDMVQLLKKYPIVWQGLLALKNDTAAVQLHFVSGNNVLAHRSLPLSEGGPPLRIAQRMRLEATQLEGVARRMTVETDYCLLLALPCGRDQEDVVSQTESLKAAFITYLQAKQAAGIINVPNPGSNQPAYVLQIFPPCEFSESHLSRLAPDLLASISNISPHLMIVIASV

Target ORF information:

RefSeq Version	NM_015001.2
Organism	Homo sapiens(human)
Definition	Homo sapiens spen family transcriptional repressor (SPEN), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_015001.2

ORF Insert Sequence:

ATGGTCCGGG AAACCAGGCA TCTCTGGGTG GGCAACTTAC CCGAGAACGT GCGGGAAGAG 
AAGATCATCG AGCATTTCAA ACGATATGGC CGCGTGGAAA GTGTCAAAAT TCTTCCCAAG 
AGGGGATCTG AAGGAGGAGT GGCTGCCTTT GTGGATTTTG TGGACATCAA AAGTGCACAG 
AAAGCTCACA ACTCGGTCAA CAAAATGGGT GACAGAGACC TACGCACGGA TTATAATGAA 
CCAGGCACCA TCCCGAGTGC TGCTCGGGGA TTGGATGATA CAGTTTCCAT AGCATCTCGT 
AGTAGAGAGG TTTCTGGGTT CAGAGGAGGT GGTGGAGGGC CTGCTTATGG TCCCCCACCG 
TCACTTCATG CACGAGAAGG ACGTTATGAG CGGAGACTTG ATGGGGCTTC AGATAACAGG 
GAGCGTGCTT ATGAACATAG TGCCTATGGA CACCATGAAC GGGGGACGGG AGGATTTGAT 
CGGACAAGAC ATTACGATCA GGATTACTAT AGAGATCCTC GAGAGCGGAC TTTACAACAT 
GGGCTCTATT ACGCTTCTCG GAGTCGAAGT CCAAATCGCT TTGATGCTCA TGACCCCCGA 
TATGAACCTA GGGCTCGCGA GCAGTTTACA CTGCCCAGTG TGGTACACAG GGATATCTAC 
AGGGATGATA TTACCCGGGA GGTACGAGGC AGAAGGCCAG AGCGGAATTA CCAGCACAGC 
AGGAGTCGGT CACCACATTC ATCCCAGTCT AGAAATCAGT CTCCTCAGAG ACTGGCTAGC 
CAAGCATCTA GACCCACAAG GTCCCCTAGC GGCAGCGGCT CTAGAAGTAG ATCCTCCAGT 
AGTGATTCAA TCAGCAGCAG CAGTAGTACC AGCAGTGACA GCAGTGATTC CAGCAGTAGT 
TCAAGTGATG ATTCTCCAGC TCGATCAGTT CAGTCTGCAG CAGTCCCTGC ACCCACTTCC 
CAGTTGCTTT CATCTCTGGA AAAAGATGAG CCCCGTAAAA GTTTTGGCAT CAAGGTCCAG 
AATCTTCCAG TACGCTCTAC AGATACAAGC CTTAAAGATG GCCTTTTCCA TGAATTTAAG 
AAATTTGGAA AAGTAACTTC AGTGCAGATA CATGGAACTT CAGAAGAGAG GTATGGTCTG 
GTATTCTTTC GGCAGCAAGA GGACCAAGAA AAAGCCTTGA CTGCATCAAA AGGAAAACTT 
TTCTTTGGCA TGCAGATTGA AGTAACAGCA TGGATAGGTC CAGAAACAGA AAGTGAAAAT 
GAATTTCGCC CCTTGGATGA AAGGATAGAT GAATTTCACC CCAAAGCAAC AAGAACTCTC 
TTTATTGGCA ACCTTGAAAA AACCACTACT TACCATGACC TTCGCAACAT CTTCCAGCGC 
TTTGGAGAAA TTGTGGATAT TGACATTAAG AAAGTAAATG GAGTTCCTCA GTATGCGTTT 
CTGCAATACT GTGATATTGC TAGCGTTTGT AAAGCTATTA AGAAGATGGA TGGGGAATAT 
CTTGGAAATA ATCGCCTCAA GCTGGGTTTT GGAAAGAGCA TGCCTACAAA CTGCGTGTGG 
CTAGATGGGC TTTCTTCGAA TGTGTCAGAT CAGTATTTAA CACGACATTT CTGCCGATAT 
GGGCCTGTGG TAAAGGTGGT GTTTGACCGC TTAAAAGGCA TGGCCCTGGT TCTCTACAAT 
GAAATTGAAT ATGCACAAGC AGCTGTAAAA GAGACCAAAG GGAGGAAAAT CGGTGGGAAT 
AAAATTAAGG TGGATTTTGC AAATCGGGAA AGTCAGCTGG CTTTTTATCA CTGCATGGAG 
AAATCTGGTC AAGACATCAG AGACTTTTAT GAAATGTTAG CCGAAAGAAG AGAGGAACGA 
AGGGCATCCT ACGACTATAA CCAAGATCGT ACATATTATG AGAGTGTTCG AACTCCAGGC 
ACTTATCCTG AGGATTCCAG GCGGGACTAT CCAGCTCGAG GGAGAGAGTT TTATTCAGAA 
TGGGAAACTT ACCAAGGAGA CTACTATGAA TCACGATACT ACGATGATCC TCGGGAATAC 
AGGGATTACA GGAATGATCC TTATGAACAA GATATTAGGG AATATAGTTA CAGGCAAAGG 
GAACGAGAAA GAGAACGTGA AAGATTTGAG TCTGACCGGG ACAGAGACCA TGAGAGGAGG 
CCGATTGAAC GAAGTCAAAG TCCTGTTCAC TTGCGACGTC CACAGAGTCC TGGAGCGTCT 
CCCTCTCAGG CAGAGAGGTT GCCGAGTGAT TCTGAGAGGA GGCTTTACAG CCGATCCTCA 
GACCGGAGTG GAAGCTGTAG CTCACTCTCC CCTCCAAGAT ATGAGAAACT GGACAAGTCT 
CGTTTGGAGC GCTATACAAA AAATGAAAAG ACAGATAAAG AACGAACTTT TGATCCGGAG 
AGAGTGGAGA GAGAGAGACG CTTAATACGG AAGGAAAAAG TGGAAAAGGA CAAAACTGAC 
AAGCAGAAAC GCAAAGGAAA GGTTCACTCC CCTAGTTCTC AGTCTTCAGA AACGGACCAA 
GAAAATGAGC GAGAGCAAAG CCCTGAAAAG CCCAGGAGTT GTAATAAACT GAGCAGAGAG 
AAAGCTGACA AAGAGGGAAT AGCGAAAAAC CGCCTGGAAC TCATGCCTTG CGTGGTTTTG 
ACTCGAGTGA AAGAGAAAGA GGGAAAGGTC ATTGACCACA CTCCTGTGGA AAAGTTGAAA 
GCCAAGCTTG ATAATGACAC TGTCAAATCT TCTGCCCTGG ACCAGAAACT TCAGGTCTCT 
CAGACGGAGC CTGCAAAATC TGACTTGTCT AAACTGGAAT CAGTTAGAAT GAAAGTACCA 
AAGGAAAAGG GGCTTTCAAG CCATGTTGAA GTGGTGGAGA AGGAAGGCAG GCTTAAAGCC 
AGGAAGCACC TCAAGCCTGA GCAGCCTGCA GATGGGGTAA GTGCTGTGGA TCTGGAGAAG 
CTGGAAGCCA GGAAAAGGCG CTTTGCAGAT TCCAATTTAA AAGCAGAAAA GCAAAAACCA 
GAGGTCAAGA AAAGCAGTCC AGAGATGGAG GATGCTCGCG TGCTTTCAAA AAAGCAGCCT 
GACGTGTCCT CTAGAGAGGT CATTCTGCTG AGGGAAGGAG AGGCTGAAAG AAAGCCTGTG 
AGGAAAGAAA TTCTTAAAAG AGAATCTAAA AAAATCAAAC TGGACAGACT TAATACTGTT 
GCCAGCCCCA AAGACTGTCA GGAGCTTGCC AGTATTTCTG TTGGGTCTGG CTCAAGGCCC 
AGCTCAGACC TACAAGCAAG ACTGGGAGAA CTAGCAGGTG AATCTGTGGA AAATCAAGAA 
GTCCAATCAA AAAAGCCCAT TCCCTCAAAA CCACAGCTCA AACAGCTGCA GGTATTAGAT 
GATCAAGGAC CAGAGAGAGA AGACGTTAGG AAAAACTATT GCAGTCTTCG TGATGAAACA 
CCTGAACGTA AATCAGGCCA AGAGAAATCA CATTCAGTAA ATACTGAAGA AAAAATTGGC 
ATTGACATCG ATCACACGCA GAGTTACCGA AAACAAATGG AACAGAGTCG TAGGAAACAG 
CAGATGGAAA TGGAAATAGC CAAGTCTGAG AAGTTTGGCA GTCCTAAAAA AGATGTAGAT 
GAATATGAAA GACGTAGCCT CGTTCACGAG GTAGGCAAAC CCCCTCAAGA TGTCACTGAT 
GACTCTCCTC CTAGCAAAAA GAAAAGGATG GATCATGTCG ATTTTGATAT CTGCACCAAG 
CGAGAACGGA ATTACAGAAG TTCACGCCAA ATCAGCGAAG ATTCTGAAAG GACTGGTGGT 
TCTCCCAGTG TCCGACATGG TTCCTTCCAT GAAGATGAGG ATCCCATAGG CTCCCCTAGG 
CTACTGTCAG TAAAAGGGTC TCCTAAAGTA GATGAAAAAG TCCTCCCCTA TTCTAACATA 
ACAGTCAGGG AAGAGTCTTT AAAATTTAAT CCTTATGATT CTAGCAGGAG AGAACAGATG 
GCAGATATGG CCAAAATAAA ACTATCTGTC TTGAATTCTG AAGATGAACT AAATCGTTGG 
GACTCTCAGA TGAAACAGGA TGCTGGCAGA TTTGATGTGA GTTTCCCAAA CAGCATAATT 
AAGAGAGATA GCCTTCGAAA AAGGTCTGTA CGAGATCTGG AACCTGGTGA GGTGCCTTCT 
GATTCTGACG AAGATGGTGA ACACAAATCC CACTCACCCA GAGCCTCTGC ATTATATGAA 
AGTTCTCGAT TGTCTTTTTT ATTGAGGGAC AGAGAAGACA AGCTACGTGA GCGAGATGAA 
AGACTCTCTA GTTCTTTAGA AAGGAACAAA TTTTACTCTT TTGCATTGGA TAAGACAATC 
ACACCAGACA CTAAAGCTTT GCTTGAAAGA GCTAAATCCC TCTCTTCATC TCGTGAAGAA 
AATTGGTCTT TTCTTGATTG GGACTCCCGA TTTGCAAATT TTCGAAACAA CAAAGATAAA 
GAAAAGGTTG ACTCTGCTCC AAGACCTATT CCATCCTGGT ACATGAAAAA GAAGAAAATT 
AGGACTGATT CAGAAGGGAA AATGGATGAT AAGAAAGAGG ACCATAAAGA AGAAGAGCAA 
GAGAGGCAGG AATTGTTTGC TTCTCGTTTT TTACACAGCT CAATCTTTGA ACAAGATTCC 
AAGCGATTGC AGCATCTAGA GAGAAAAGAG GAAGATTCTG ACTTCATTTC TGGTAGGATC 
TATGGGAAGC AGACATCTGA GGGAGCAAAC AGCACAACTG ATTCCATTCA AGAACCAGTA 
GTTCTGTTCC ATAGCAGATT TATGGAGCTC ACACGGATGC AACAGAAAGA AAAAGAAAAA 
GACCAGAAAC CCAAAGAGGT TGAGAAACAG GAAGATACAG AGAATCATCC CAAGACCCCA 
GAATCTGCTC CTGAGAATAA AGATTCAGAA CTGAAAACTC CACCTTCCGT TGGGCCTCCA 
AGTGTCACAG TCGTAACTCT AGAATCAGCC CCATCAGCAC TAGAGAAGAC CACTGGTGAC 
AAAACGGTAG AGGCGCCTTT GGTAACAGAA GAGAAGACTG TGGAGCCAGC TACCGTCTCA 
GAAGAAGCAA AGCCTGCATC TGAACCTGCT CCTGCCCCTG TGGAACAGCT GGAACAAGTA 
GACCTGCCCC CAGGAGCAGA CCCCGATAAA GAAGCTGCCA TGATGCCTGC GGGTGTTGAG 
GAAGGTTCAT CAGGTGACCA GCCGCCTTAT CTGGATGCCA AGCCTCCAAC TCCCGGGGCC 
TCGTTTTCCC AGGCAGAGAG CAACGTAGAT CCAGAGCCTG ACAGTACCCA GCCACTTTCA 
AAACCAGCTC AGAAGTCTGA GGAAGCCAAT GAGCCAAAGG CCGAAAAGCC AGACGCCACT 
GCAGATGCTG AGCCTGATGC AAACCAGAAA GCCGAAGCTG CTCCTGAGTC TCAGCCCCCA 
GCTTCTGAAG ATTTAGAGGT TGATCCTCCA GTTGCTGCAA AGGATAAAAA GCCAAACAAA 
AGCAAGCGTT CAAAGACCCC TGTTCAGGCA GCTGCAGTGA GTATCGTGGA GAAGCCCGTC 
ACAAGGAAGA GTGAGAGGAT AGACCGGGAA AAACTCAAGC GGTCCAATTC TCCTCGGGGA 
GAAGCACAGA AGCTTTTGGA ATTGAAGATG GAGGCAGAGA AGATTACAAG GACTGCTTCT 
AAAAACTCTG CTGCAGACCT TGAACATCCC GAACCAAGTT TGCCTCTCAG CCGAACAAGG 
CGCCGGAATG TAAGGAGCGT CTATGCAACC ATGGGTGACC ATGAAAACCG CTCTCCTGTC 
AAAGAGCCCG TTGAGCAACC AAGAGTGACC AGAAAGAGAT TGGAGCGAGA GCTTCAGGAG 
GCTGCAGCGG TTCCCACCAC CCCTCGGAGG GGAAGGCCTC CAAAGACACG CCGGCGAGCC 
GATGAAGAGG AGGAGAACGA GGCCAAGGAA CCTGCAGAAA CACTCAAGCC ACCTGAGGGA 
TGGCGGTCGC CAAGGTCCCA GAAAACTGCA GCTGGTGGTG GACCCCAAGG GAAAAAGGGA 
AAAAATGAAC CGAAGGTGGA TGCTACACGT CCTGAGGCCA CCACTGAGGT GGGCCCCCAA 
ATAGGCGTGA AAGAGAGCTC CATGGAACCC AAGGCTGCTG AGGAGGAGGC AGGGAGTGAA 
CAGAAACGTG ACAGAAAAGA TGCTGGCACA GACAAAAACC CCCCTGAAAC CGCCCCTGTT 
GAAGTTGTAG AGAAAAAACC GGCCCCTGAA AAAAACTCCA AATCAAAGAG AGGAAGATCT 
CGAAACTCCA GGTTAGCAGT GGACAAATCT GCAAGTCTGA AAAATGTGGA TGCTGCTGTC 
AGTCCCAGGG GGGCTGCAGC ACAGGCAGGG GAGAGGGAAT CTGGGGTGGT GGCAGTCTCC 
CCTGAGAAAA GTGAGAGTCC CCAAAAGGAG GATGGTTTAT CATCCCAGTT GAAAAGTGAT 
CCAGTTGATC CAGACAAGGA ACCAGAGAAA GAAGACGTGT CTGCCTCTGG GCCGTCCCCA 
GAAGCCACCC AGTTAGCCAA GCAGATGGAG CTGGAGCAGG CCGTGGAACA CATCGCAAAG 
CTCGCTGAGG CCTCTGCCTC TGCTGCCTAT AAGGCAGATG CACCAGAGGG CCTTGCCCCA 
GAGGACAGGG ACAAGCCTGC ACACCAAGCA AGTGAAACAG AGCTGGCTGC GGCCATCGGC 
TCCATCATCA ATGACATTTC TGGGGAGCCA GAAAACTTCC CAGCACCTCC ACCTTATCCT 
GGAGAATCCC AGACAGATCT GCAACCCCCC GCAGGTGCAC AGGCGCTGCA GCCTTCTGAG 
GAAGGAATGG AGACAGATGA GGCTGTATCT GGCATCCTGG AAACTGAGGC TGCTACAGAA 
TCTTCTAGGC CTCCAGTCAA TGCTCCTGAC CCCTCAGCCG GCCCAACAGA TACCAAGGAA 
GCCAGAGGAA ATAGCAGTGA AACCTCACAC TCAGTGCCAG AAGCCAAAGG GTCTAAAGAA 
GTGGAAGTCA CTCTTGTTCG GAAAGACAAA GGGCGCCAGA AAACAACCCG ATCACGCCGC 
AAGCGAAACA CAAACAAGAA AGTGGTGGCT CCTGTAGAGA GCCATGTCCC TGAATCCAAC 
CAAGCTCAAG GTGAGAGTCC TGCTGCAAAT GAGGGGACAA CAGTACAGCA CCCCGAAGCC 
CCACAGGAAG AAAAGCAGAG TGAGAAACCC CATTCCACTC CTCCTCAGTC ATGTACTTCT 
GACCTAAGCA AGATTCCCTC CACAGAGAAT TCGTCCCAAG AAATCAGTGT TGAGGAAAGG 
ACTCCAACCA AAGCATCTGT GCCCCCAGAC CTTCCCCCAC CTCCCCAGCC AGCACCGGTG 
GATGAGGAGC CTCAAGCCAG GTTCAGGGTG CATTCCATCA TTGAAAGTGA CCCGGTGACC 
CCACCCAGCG ATCCAAGCAT CCCCATACCC ACACTGCCTT CTGTAACTGC AGCAAAGCTC 
TCACCTCCTG TCGCCTCTGG GGGGATCCCA CACCAGAGCC CCCCTACTAA GGTGACAGAG 
TGGATCACAA GGCAGGAGGA GCCACGGGCT CAGTCTACTC CATCTCCAGC TCTTCCCCCA 
GACACAAAGG CCTCTGATGT TGACACCAGC TCCAGCACCC TGAGGAAGAT TCTCATGGAC 
CCCAAGTATG TGTCTGCCAC AAGTGTCACT TCCACAAGTG TCACCACAGC CATTGCAGAG 
CCTGTCAGTG CTGCCCCTTG CCTACATGAG GCCCCGCCCC CGCCAGTTGA CTCTAAAAAG 
CCTTTAGAAG AAAAAACAGC ACCTCCAGTG ACAAACAACT CTGAGATACA AGCCTCGGAG 
GTGCTGGTAG CTGCTGACAA GGAAAAGGTG GCTCCAGTCA TTGCTCCCAA AATTACCTCT 
GTTATTAGCC GGATGCCTGT CAGCATTGAC CTGGAAAATT CACAGAAGAT AACCTTGGCA 
AAACCAGCTC CTCAAACCCT CACTGGTCTG GTGAGCGCAC TCACTGGCCT GGTGAACGTC 
TCCCTGGTCC CGGTGAATGC CCTGAAAGGC CCCGTGAAGG GCTCAGTGAC CACACTGAAA 
AGTTTGGTGA GCACCCCTGC TGGGCCCGTG AACGTCCTGA AAGGGCCTGT GAATGTTCTT 
ACGGGGCCAG TGAATGTTCT CACCACTCCA GTGAACGCCA CGGTGGGCAC AGTGAATGCC 
GCCCCAGGCA CAGTCAATGC CGCTGCGAGT GCAGTGAATG CCACAGCAAG TGCAGTGACC 
GTCACAGCGG GTGCGGTTAC TGCTGCATCT GGTGGTGTAA CGGCCACAAC AGGCACGGTG 
ACAATGGCAG GGGCAGTGAT TGCGCCGTCA ACAAAGTGCA AACAGAGAGC GAGTGCTAAT 
GAAAACAGTC GGTTCCACCC AGGGTCCATG CCTGTGATCG ACGATCGTCC GGCAGACGCG 
GGCTCAGGGG CGGGGCTGCG TGTGAACACT TCTGAAGGGG TTGTGCTCCT GAGTTACTCA 
GGGCAGAAGA CCGAAGGCCC ACAGCGGATC AGCGCCAAGA TCAGCCAGAT CCCCCCGGCC 
AGTGCAATGG ACATTGAATT TCAGCAGTCA GTGTCCAAGT CCCAGGTCAA ACCTGATTCT 
GTCACAGCAT CGCAGCCTCC ATCCAAAGGC CCTCAAGCTC CTGCAGGCTA TGCGAACGTG 
GCCACCCATT CCACGTTGGT ACTGACCGCC CAGACATATA ATGCCTCTCC TGTGATTTCG 
TCTGTGAAGG CCGATAGGCC ATCCTTGGAG AAGCCCGAGC CCATTCACCT CTCGGTGTCC 
ACGCCTGTCA CCCAGGGAGG CACAGTGAAG GTTCTCACCC AGGGGATCAA CACACCCCCT 
GTGCTGGTTC ACAACCAGCT GGTCCTCACC CCAAGCATTG TCACCACAAA CAAGAAGCTT 
GCTGACCCCG TCACCCTTAA AATCGAGACC AAGGTCCTTC AGCCGGCCAA CCTGGGGTCC 
ACGCTCACGC CCCACCACCC TCCTGCTCTG CCCAGCAAAC TGCCTACAGA AGTCAACCAT 
GTCCCCTCGG GGCCCAGCAT CCCAGCAGAT CGAACTGTCT CCCATTTGGC AGCTGCAAAG 
CTAGATGCTC ATTCTCCTCG ACCAAGTGGA CCCGGGCCAT CCTCATTCCC AAGGGCAAGC 
CACCCCAGCA GTACTGCATC TACGGCGCTC TCCACCAACG CCACAGTCAT GCTGGCTGCA 
GGCATCCCAG TGCCCCAGTT CATCTCCAGC ATCCACCCAG AGCAGTCTGT CATCATGCCA 
CCCCACAGCA TCACCCAGAC TGTGTCCCTG AGCCACCTCT CCCAGGGCGA GGTGAGAATG 
AACACTCCCA CGCTGCCCAG TATCACCTAC AGCATCCGGC CAGAAGCGCT TCACTCTCCT 
CGGGCTCCGC TGCAGCCCCA GCAAATAGAG GTCAGGGCCC CACAGCGTGC CAGCACCCCG 
CAGCCAGCCC CAGCTGGTGT GCCTGCACTG GCCTCCCAGC ACCCTCCCGA GGAGGAAGTG 
CATTATCACC TTCCTGTCGC TCGAGCCACA GCCCCTGTGC AGTCAGAGGT ACTAGTCATG 
CAGTCTGAGT ACCGACTGCA CCCCTATACT GTGCCACGGG ATGTGAGGAT CATGGTGCAT 
CCACATGTGA CGGCAGTCAG CGAGCAGCCC AGGGCCGCGG ATGGGGTGGT GAAGGTGCCA 
CCAGCCAGCA AGGCCCCTCA GCAGCCAGGG AAGGAAGCTG CCAAGACACC AGATGCCAAA 
GCTGCCCCCA CCCCCACCCC TGCCCCCGTC CCTGTCCCTG TCCCCCTTCC TGCCCCTGCT 
CCTGCCCCTC ATGGTGAGGC CCGTATCCTC ACAGTTACCC CCAGTAACCA ACTCCAGGGG 
CTGCCTCTGA CCCCTCCTGT GGTGGTGACC CATGGGGTGC AGATTGTGCA CTCCAGCGGG 
GAGCTGTTTC AAGAGTACCG GTACGGCGAC ATCCGCACCT ACCACCCCCC GGCCCAGCTC 
ACACACACTC AGTTTCCCGC CGCTTCCTCT GTTGGCCTGC CTTCCCGGAC CAAGACAGCT 
GCTCAGGGCC CTCCTCCTGA AGGTGAGCCC CTGCAGCCTC CTCAGCCTGT GCAGTCCACA 
CAGCCTGCCC AGCCTGCACC ACCCTGCCCG CCCTCCCAGC TCGGTCAGCC CGGCCAGCCA 
CCAAGCAGCA AGATGCCTCA AGTGTCCCAG GAGGCAAAGG GGACCCAGAC GGGAGTAGAG 
CAGCCTCGCC TCCCAGCTGG ACCTGCAAAC AGGCCACCTG AGCCTCACAC CCAGGTTCAG 
AGGGCACAAG CAGAAACAGG CCCGACTTCC TTCCCCTCCC CTGTGTCTGT CTCCATGAAG 
CCTGACCTTC CAGTCTCTCT TCCCACTCAG ACTGCCCCAA AACAGCCGTT GTTTGTCCCA 
ACAACCTCTG GCCCCAGCAC CCCACCAGGA CTGGTTCTGC CACACACTGA ATTCCAGCCA 
GCCCCCAAAC AAGATTCCTC TCCACACCTG ACTTCCCAGA GACCCGTGGA TATGGTTCAA 
CTTCTGAAGA AGTACCCCAT CGTGTGGCAG GGCCTGCTGG CCCTCAAGAA TGACACAGCT 
GCTGTGCAGC TCCACTTCGT CTCTGGCAAC AACGTCCTGG CCCATCGGTC CCTGCCCCTT 
TCTGAAGGAG GGCCCCCACT AAGGATCGCC CAGAGGATGC GGCTGGAGGC AACGCAGCTG 
GAAGGGGTTG CCCGAAGGAT GACGGTGGAG ACAGATTACT GTCTGCTGCT GGCTCTGCCC 
TGTGGCCGTG ACCAAGAGGA TGTTGTGAGC CAGACCGAGT CCCTCAAGGC TGCCTTCATC 
ACTTACCTGC AGGCCAAGCA GGCGGCAGGG ATCATCAACG TTCCCAACCC TGGCTCCAAT 
CAGCCTGCCT ACGTGCTGCA GATCTTCCCG CCCTGTGAGT TCTCTGAGAG TCACCTGTCC 
CGCCTGGCCC CTGACCTCCT TGCCAGCATC TCCAACATCT CTCCCCACCT CATGATTGTC 
ATTGCCTCCG TGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SPEN ( NM_015001.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_055816.2 Homo sapiens spen family transcriptional repressor (SPEN), mRNA.

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CloneID	OHu13146
Clone ID Related Accession (Same CDS sequence)	NM_015001.2 , NM_015001.3
Accession Version	NM_015001.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 10995bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-21
Organism	Homo sapiens(human)
Product	msx2-interacting protein
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL450998.19, AF356524.1 and AL034555.2. This sequence is a reference standard in the RefSeqGene project. On Nov 22, 2018 this sequence version replaced NM_015001.2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF356524.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000375759.8/ ENSP00000364912.3 RefSeq Select criteria :: based on single protein-coding transcript ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGGTCCGGG AAACCAGGCA TCTCTGGGTG GGCAACTTAC CCGAGAACGT GCGGGAAGAG 
AAGATCATCG AGCATTTCAA ACGATATGGC CGCGTGGAAA GTGTCAAAAT TCTTCCCAAG 
AGGGGATCTG AAGGAGGAGT GGCTGCCTTT GTGGATTTTG TGGACATCAA AAGTGCACAG 
AAAGCTCACA ACTCGGTCAA CAAAATGGGT GACAGAGACC TACGCACGGA TTATAATGAA 
CCAGGCACCA TCCCGAGTGC TGCTCGGGGA TTGGATGATA CAGTTTCCAT AGCATCTCGT 
AGTAGAGAGG TTTCTGGGTT CAGAGGAGGT GGTGGAGGGC CTGCTTATGG TCCCCCACCG 
TCACTTCATG CACGAGAAGG ACGTTATGAG CGGAGACTTG ATGGGGCTTC AGATAACAGG 
GAGCGTGCTT ATGAACATAG TGCCTATGGA CACCATGAAC GGGGGACGGG AGGATTTGAT 
CGGACAAGAC ATTACGATCA GGATTACTAT AGAGATCCTC GAGAGCGGAC TTTACAACAT 
GGGCTCTATT ACGCTTCTCG GAGTCGAAGT CCAAATCGCT TTGATGCTCA TGACCCCCGA 
TATGAACCTA GGGCTCGCGA GCAGTTTACA CTGCCCAGTG TGGTACACAG GGATATCTAC 
AGGGATGATA TTACCCGGGA GGTACGAGGC AGAAGGCCAG AGCGGAATTA CCAGCACAGC 
AGGAGTCGGT CACCACATTC ATCCCAGTCT AGAAATCAGT CTCCTCAGAG ACTGGCTAGC 
CAAGCATCTA GACCCACAAG GTCCCCTAGC GGCAGCGGCT CTAGAAGTAG ATCCTCCAGT 
AGTGATTCAA TCAGCAGCAG CAGTAGTACC AGCAGTGACA GCAGTGATTC CAGCAGTAGT 
TCAAGTGATG ATTCTCCAGC TCGATCAGTT CAGTCTGCAG CAGTCCCTGC ACCCACTTCC 
CAGTTGCTTT CATCTCTGGA AAAAGATGAG CCCCGTAAAA GTTTTGGCAT CAAGGTCCAG 
AATCTTCCAG TACGCTCTAC AGATACAAGC CTTAAAGATG GCCTTTTCCA TGAATTTAAG 
AAATTTGGAA AAGTAACTTC AGTGCAGATA CATGGAACTT CAGAAGAGAG GTATGGTCTG 
GTATTCTTTC GGCAGCAAGA GGACCAAGAA AAAGCCTTGA CTGCATCAAA AGGAAAACTT 
TTCTTTGGCA TGCAGATTGA AGTAACAGCA TGGATAGGTC CAGAAACAGA AAGTGAAAAT 
GAATTTCGCC CCTTGGATGA AAGGATAGAT GAATTTCACC CCAAAGCAAC AAGAACTCTC 
TTTATTGGCA ACCTTGAAAA AACCACTACT TACCATGACC TTCGCAACAT CTTCCAGCGC 
TTTGGAGAAA TTGTGGATAT TGACATTAAG AAAGTAAATG GAGTTCCTCA GTATGCGTTT 
CTGCAATACT GTGATATTGC TAGCGTTTGT AAAGCTATTA AGAAGATGGA TGGGGAATAT 
CTTGGAAATA ATCGCCTCAA GCTGGGTTTT GGAAAGAGCA TGCCTACAAA CTGCGTGTGG 
CTAGATGGGC TTTCTTCGAA TGTGTCAGAT CAGTATTTAA CACGACATTT CTGCCGATAT 
GGGCCTGTGG TAAAGGTGGT GTTTGACCGC TTAAAAGGCA TGGCCCTGGT TCTCTACAAT 
GAAATTGAAT ATGCACAAGC AGCTGTAAAA GAGACCAAAG GGAGGAAAAT CGGTGGGAAT 
AAAATTAAGG TGGATTTTGC AAATCGGGAA AGTCAGCTGG CTTTTTATCA CTGCATGGAG 
AAATCTGGTC AAGACATCAG AGACTTTTAT GAAATGTTAG CCGAAAGAAG AGAGGAACGA 
AGGGCATCCT ACGACTATAA CCAAGATCGT ACATATTATG AGAGTGTTCG AACTCCAGGC 
ACTTATCCTG AGGATTCCAG GCGGGACTAT CCAGCTCGAG GGAGAGAGTT TTATTCAGAA 
TGGGAAACTT ACCAAGGAGA CTACTATGAA TCACGATACT ACGATGATCC TCGGGAATAC 
AGGGATTACA GGAATGATCC TTATGAACAA GATATTAGGG AATATAGTTA CAGGCAAAGG 
GAACGAGAAA GAGAACGTGA AAGATTTGAG TCTGACCGGG ACAGAGACCA TGAGAGGAGG 
CCGATTGAAC GAAGTCAAAG TCCTGTTCAC TTGCGACGTC CACAGAGTCC TGGAGCGTCT 
CCCTCTCAGG CAGAGAGGTT GCCGAGTGAT TCTGAGAGGA GGCTTTACAG CCGATCCTCA 
GACCGGAGTG GAAGCTGTAG CTCACTCTCC CCTCCAAGAT ATGAGAAACT GGACAAGTCT 
CGTTTGGAGC GCTATACAAA AAATGAAAAG ACAGATAAAG AACGAACTTT TGATCCGGAG 
AGAGTGGAGA GAGAGAGACG CTTAATACGG AAGGAAAAAG TGGAAAAGGA CAAAACTGAC 
AAGCAGAAAC GCAAAGGAAA GGTTCACTCC CCTAGTTCTC AGTCTTCAGA AACGGACCAA 
GAAAATGAGC GAGAGCAAAG CCCTGAAAAG CCCAGGAGTT GTAATAAACT GAGCAGAGAG 
AAAGCTGACA AAGAGGGAAT AGCGAAAAAC CGCCTGGAAC TCATGCCTTG CGTGGTTTTG 
ACTCGAGTGA AAGAGAAAGA GGGAAAGGTC ATTGACCACA CTCCTGTGGA AAAGTTGAAA 
GCCAAGCTTG ATAATGACAC TGTCAAATCT TCTGCCCTGG ACCAGAAACT TCAGGTCTCT 
CAGACGGAGC CTGCAAAATC TGACTTGTCT AAACTGGAAT CAGTTAGAAT GAAAGTACCA 
AAGGAAAAGG GGCTTTCAAG CCATGTTGAA GTGGTGGAGA AGGAAGGCAG GCTTAAAGCC 
AGGAAGCACC TCAAGCCTGA GCAGCCTGCA GATGGGGTAA GTGCTGTGGA TCTGGAGAAG 
CTGGAAGCCA GGAAAAGGCG CTTTGCAGAT TCCAATTTAA AAGCAGAAAA GCAAAAACCA 
GAGGTCAAGA AAAGCAGTCC AGAGATGGAG GATGCTCGCG TGCTTTCAAA AAAGCAGCCT 
GACGTGTCCT CTAGAGAGGT CATTCTGCTG AGGGAAGGAG AGGCTGAAAG AAAGCCTGTG 
AGGAAAGAAA TTCTTAAAAG AGAATCTAAA AAAATCAAAC TGGACAGACT TAATACTGTT 
GCCAGCCCCA AAGACTGTCA GGAGCTTGCC AGTATTTCTG TTGGGTCTGG CTCAAGGCCC 
AGCTCAGACC TACAAGCAAG ACTGGGAGAA CTAGCAGGTG AATCTGTGGA AAATCAAGAA 
GTCCAATCAA AAAAGCCCAT TCCCTCAAAA CCACAGCTCA AACAGCTGCA GGTATTAGAT 
GATCAAGGAC CAGAGAGAGA AGACGTTAGG AAAAACTATT GCAGTCTTCG TGATGAAACA 
CCTGAACGTA AATCAGGCCA AGAGAAATCA CATTCAGTAA ATACTGAAGA AAAAATTGGC 
ATTGACATCG ATCACACGCA GAGTTACCGA AAACAAATGG AACAGAGTCG TAGGAAACAG 
CAGATGGAAA TGGAAATAGC CAAGTCTGAG AAGTTTGGCA GTCCTAAAAA AGATGTAGAT 
GAATATGAAA GACGTAGCCT CGTTCACGAG GTAGGCAAAC CCCCTCAAGA TGTCACTGAT 
GACTCTCCTC CTAGCAAAAA GAAAAGGATG GATCATGTCG ATTTTGATAT CTGCACCAAG 
CGAGAACGGA ATTACAGAAG TTCACGCCAA ATCAGCGAAG ATTCTGAAAG GACTGGTGGT 
TCTCCCAGTG TCCGACATGG TTCCTTCCAT GAAGATGAGG ATCCCATAGG CTCCCCTAGG 
CTACTGTCAG TAAAAGGGTC TCCTAAAGTA GATGAAAAAG TCCTCCCCTA TTCTAACATA 
ACAGTCAGGG AAGAGTCTTT AAAATTTAAT CCTTATGATT CTAGCAGGAG AGAACAGATG 
GCAGATATGG CCAAAATAAA ACTATCTGTC TTGAATTCTG AAGATGAACT AAATCGTTGG 
GACTCTCAGA TGAAACAGGA TGCTGGCAGA TTTGATGTGA GTTTCCCAAA CAGCATAATT 
AAGAGAGATA GCCTTCGAAA AAGGTCTGTA CGAGATCTGG AACCTGGTGA GGTGCCTTCT 
GATTCTGACG AAGATGGTGA ACACAAATCC CACTCACCCA GAGCCTCTGC ATTATATGAA 
AGTTCTCGAT TGTCTTTTTT ATTGAGGGAC AGAGAAGACA AGCTACGTGA GCGAGATGAA 
AGACTCTCTA GTTCTTTAGA AAGGAACAAA TTTTACTCTT TTGCATTGGA TAAGACAATC 
ACACCAGACA CTAAAGCTTT GCTTGAAAGA GCTAAATCCC TCTCTTCATC TCGTGAAGAA 
AATTGGTCTT TTCTTGATTG GGACTCCCGA TTTGCAAATT TTCGAAACAA CAAAGATAAA 
GAAAAGGTTG ACTCTGCTCC AAGACCTATT CCATCCTGGT ACATGAAAAA GAAGAAAATT 
AGGACTGATT CAGAAGGGAA AATGGATGAT AAGAAAGAGG ACCATAAAGA AGAAGAGCAA 
GAGAGGCAGG AATTGTTTGC TTCTCGTTTT TTACACAGCT CAATCTTTGA ACAAGATTCC 
AAGCGATTGC AGCATCTAGA GAGAAAAGAG GAAGATTCTG ACTTCATTTC TGGTAGGATC 
TATGGGAAGC AGACATCTGA GGGAGCAAAC AGCACAACTG ATTCCATTCA AGAACCAGTA 
GTTCTGTTCC ATAGCAGATT TATGGAGCTC ACACGGATGC AACAGAAAGA AAAAGAAAAA 
GACCAGAAAC CCAAAGAGGT TGAGAAACAG GAAGATACAG AGAATCATCC CAAGACCCCA 
GAATCTGCTC CTGAGAATAA AGATTCAGAA CTGAAAACTC CACCTTCCGT TGGGCCTCCA 
AGTGTCACAG TCGTAACTCT AGAATCAGCC CCATCAGCAC TAGAGAAGAC CACTGGTGAC 
AAAACGGTAG AGGCGCCTTT GGTAACAGAA GAGAAGACTG TGGAGCCAGC TACCGTCTCA 
GAAGAAGCAA AGCCTGCATC TGAACCTGCT CCTGCCCCTG TGGAACAGCT GGAACAAGTA 
GACCTGCCCC CAGGAGCAGA CCCCGATAAA GAAGCTGCCA TGATGCCTGC GGGTGTTGAG 
GAAGGTTCAT CAGGTGACCA GCCGCCTTAT CTGGATGCCA AGCCTCCAAC TCCCGGGGCC 
TCGTTTTCCC AGGCAGAGAG CAACGTAGAT CCAGAGCCTG ACAGTACCCA GCCACTTTCA 
AAACCAGCTC AGAAGTCTGA GGAAGCCAAT GAGCCAAAGG CCGAAAAGCC AGACGCCACT 
GCAGATGCTG AGCCTGATGC AAACCAGAAA GCCGAAGCTG CTCCTGAGTC TCAGCCCCCA 
GCTTCTGAAG ATTTAGAGGT TGATCCTCCA GTTGCTGCAA AGGATAAAAA GCCAAACAAA 
AGCAAGCGTT CAAAGACCCC TGTTCAGGCA GCTGCAGTGA GTATCGTGGA GAAGCCCGTC 
ACAAGGAAGA GTGAGAGGAT AGACCGGGAA AAACTCAAGC GGTCCAATTC TCCTCGGGGA 
GAAGCACAGA AGCTTTTGGA ATTGAAGATG GAGGCAGAGA AGATTACAAG GACTGCTTCT 
AAAAACTCTG CTGCAGACCT TGAACATCCC GAACCAAGTT TGCCTCTCAG CCGAACAAGG 
CGCCGGAATG TAAGGAGCGT CTATGCAACC ATGGGTGACC ATGAAAACCG CTCTCCTGTC 
AAAGAGCCCG TTGAGCAACC AAGAGTGACC AGAAAGAGAT TGGAGCGAGA GCTTCAGGAG 
GCTGCAGCGG TTCCCACCAC CCCTCGGAGG GGAAGGCCTC CAAAGACACG CCGGCGAGCC 
GATGAAGAGG AGGAGAACGA GGCCAAGGAA CCTGCAGAAA CACTCAAGCC ACCTGAGGGA 
TGGCGGTCGC CAAGGTCCCA GAAAACTGCA GCTGGTGGTG GACCCCAAGG GAAAAAGGGA 
AAAAATGAAC CGAAGGTGGA TGCTACACGT CCTGAGGCCA CCACTGAGGT GGGCCCCCAA 
ATAGGCGTGA AAGAGAGCTC CATGGAACCC AAGGCTGCTG AGGAGGAGGC AGGGAGTGAA 
CAGAAACGTG ACAGAAAAGA TGCTGGCACA GACAAAAACC CCCCTGAAAC CGCCCCTGTT 
GAAGTTGTAG AGAAAAAACC GGCCCCTGAA AAAAACTCCA AATCAAAGAG AGGAAGATCT 
CGAAACTCCA GGTTAGCAGT GGACAAATCT GCAAGTCTGA AAAATGTGGA TGCTGCTGTC 
AGTCCCAGGG GGGCTGCAGC ACAGGCAGGG GAGAGGGAAT CTGGGGTGGT GGCAGTCTCC 
CCTGAGAAAA GTGAGAGTCC CCAAAAGGAG GATGGTTTAT CATCCCAGTT GAAAAGTGAT 
CCAGTTGATC CAGACAAGGA ACCAGAGAAA GAAGACGTGT CTGCCTCTGG GCCGTCCCCA 
GAAGCCACCC AGTTAGCCAA GCAGATGGAG CTGGAGCAGG CCGTGGAACA CATCGCAAAG 
CTCGCTGAGG CCTCTGCCTC TGCTGCCTAT AAGGCAGATG CACCAGAGGG CCTTGCCCCA 
GAGGACAGGG ACAAGCCTGC ACACCAAGCA AGTGAAACAG AGCTGGCTGC GGCCATCGGC 
TCCATCATCA ATGACATTTC TGGGGAGCCA GAAAACTTCC CAGCACCTCC ACCTTATCCT 
GGAGAATCCC AGACAGATCT GCAACCCCCC GCAGGTGCAC AGGCGCTGCA GCCTTCTGAG 
GAAGGAATGG AGACAGATGA GGCTGTATCT GGCATCCTGG AAACTGAGGC TGCTACAGAA 
TCTTCTAGGC CTCCAGTCAA TGCTCCTGAC CCCTCAGCCG GCCCAACAGA TACCAAGGAA 
GCCAGAGGAA ATAGCAGTGA AACCTCACAC TCAGTGCCAG AAGCCAAAGG GTCTAAAGAA 
GTGGAAGTCA CTCTTGTTCG GAAAGACAAA GGGCGCCAGA AAACAACCCG ATCACGCCGC 
AAGCGAAACA CAAACAAGAA AGTGGTGGCT CCTGTAGAGA GCCATGTCCC TGAATCCAAC 
CAAGCTCAAG GTGAGAGTCC TGCTGCAAAT GAGGGGACAA CAGTACAGCA CCCCGAAGCC 
CCACAGGAAG AAAAGCAGAG TGAGAAACCC CATTCCACTC CTCCTCAGTC ATGTACTTCT 
GACCTAAGCA AGATTCCCTC CACAGAGAAT TCGTCCCAAG AAATCAGTGT TGAGGAAAGG 
ACTCCAACCA AAGCATCTGT GCCCCCAGAC CTTCCCCCAC CTCCCCAGCC AGCACCGGTG 
GATGAGGAGC CTCAAGCCAG GTTCAGGGTG CATTCCATCA TTGAAAGTGA CCCGGTGACC 
CCACCCAGCG ATCCAAGCAT CCCCATACCC ACACTGCCTT CTGTAACTGC AGCAAAGCTC 
TCACCTCCTG TCGCCTCTGG GGGGATCCCA CACCAGAGCC CCCCTACTAA GGTGACAGAG 
TGGATCACAA GGCAGGAGGA GCCACGGGCT CAGTCTACTC CATCTCCAGC TCTTCCCCCA 
GACACAAAGG CCTCTGATGT TGACACCAGC TCCAGCACCC TGAGGAAGAT TCTCATGGAC 
CCCAAGTATG TGTCTGCCAC AAGTGTCACT TCCACAAGTG TCACCACAGC CATTGCAGAG 
CCTGTCAGTG CTGCCCCTTG CCTACATGAG GCCCCGCCCC CGCCAGTTGA CTCTAAAAAG 
CCTTTAGAAG AAAAAACAGC ACCTCCAGTG ACAAACAACT CTGAGATACA AGCCTCGGAG 
GTGCTGGTAG CTGCTGACAA GGAAAAGGTG GCTCCAGTCA TTGCTCCCAA AATTACCTCT 
GTTATTAGCC GGATGCCTGT CAGCATTGAC CTGGAAAATT CACAGAAGAT AACCTTGGCA 
AAACCAGCTC CTCAAACCCT CACTGGTCTG GTGAGCGCAC TCACTGGCCT GGTGAACGTC 
TCCCTGGTCC CGGTGAATGC CCTGAAAGGC CCCGTGAAGG GCTCAGTGAC CACACTGAAA 
AGTTTGGTGA GCACCCCTGC TGGGCCCGTG AACGTCCTGA AAGGGCCTGT GAATGTTCTT 
ACGGGGCCAG TGAATGTTCT CACCACTCCA GTGAACGCCA CGGTGGGCAC AGTGAATGCC 
GCCCCAGGCA CAGTCAATGC CGCTGCGAGT GCAGTGAATG CCACAGCAAG TGCAGTGACC 
GTCACAGCGG GTGCGGTTAC TGCTGCATCT GGTGGTGTAA CGGCCACAAC AGGCACGGTG 
ACAATGGCAG GGGCAGTGAT TGCGCCGTCA ACAAAGTGCA AACAGAGAGC GAGTGCTAAT 
GAAAACAGTC GGTTCCACCC AGGGTCCATG CCTGTGATCG ACGATCGTCC GGCAGACGCG 
GGCTCAGGGG CGGGGCTGCG TGTGAACACT TCTGAAGGGG TTGTGCTCCT GAGTTACTCA 
GGGCAGAAGA CCGAAGGCCC ACAGCGGATC AGCGCCAAGA TCAGCCAGAT CCCCCCGGCC 
AGTGCAATGG ACATTGAATT TCAGCAGTCA GTGTCCAAGT CCCAGGTCAA ACCTGATTCT 
GTCACAGCAT CGCAGCCTCC ATCCAAAGGC CCTCAAGCTC CTGCAGGCTA TGCGAACGTG 
GCCACCCATT CCACGTTGGT ACTGACCGCC CAGACATATA ATGCCTCTCC TGTGATTTCG 
TCTGTGAAGG CCGATAGGCC ATCCTTGGAG AAGCCCGAGC CCATTCACCT CTCGGTGTCC 
ACGCCTGTCA CCCAGGGAGG CACAGTGAAG GTTCTCACCC AGGGGATCAA CACACCCCCT 
GTGCTGGTTC ACAACCAGCT GGTCCTCACC CCAAGCATTG TCACCACAAA CAAGAAGCTT 
GCTGACCCCG TCACCCTTAA AATCGAGACC AAGGTCCTTC AGCCGGCCAA CCTGGGGTCC 
ACGCTCACGC CCCACCACCC TCCTGCTCTG CCCAGCAAAC TGCCTACAGA AGTCAACCAT 
GTCCCCTCGG GGCCCAGCAT CCCAGCAGAT CGAACTGTCT CCCATTTGGC AGCTGCAAAG 
CTAGATGCTC ATTCTCCTCG ACCAAGTGGA CCCGGGCCAT CCTCATTCCC AAGGGCAAGC 
CACCCCAGCA GTACTGCATC TACGGCGCTC TCCACCAACG CCACAGTCAT GCTGGCTGCA 
GGCATCCCAG TGCCCCAGTT CATCTCCAGC ATCCACCCAG AGCAGTCTGT CATCATGCCA 
CCCCACAGCA TCACCCAGAC TGTGTCCCTG AGCCACCTCT CCCAGGGCGA GGTGAGAATG 
AACACTCCCA CGCTGCCCAG TATCACCTAC AGCATCCGGC CAGAAGCGCT TCACTCTCCT 
CGGGCTCCGC TGCAGCCCCA GCAAATAGAG GTCAGGGCCC CACAGCGTGC CAGCACCCCG 
CAGCCAGCCC CAGCTGGTGT GCCTGCACTG GCCTCCCAGC ACCCTCCCGA GGAGGAAGTG 
CATTATCACC TTCCTGTCGC TCGAGCCACA GCCCCTGTGC AGTCAGAGGT ACTAGTCATG 
CAGTCTGAGT ACCGACTGCA CCCCTATACT GTGCCACGGG ATGTGAGGAT CATGGTGCAT 
CCACATGTGA CGGCAGTCAG CGAGCAGCCC AGGGCCGCGG ATGGGGTGGT GAAGGTGCCA 
CCAGCCAGCA AGGCCCCTCA GCAGCCAGGG AAGGAAGCTG CCAAGACACC AGATGCCAAA 
GCTGCCCCCA CCCCCACCCC TGCCCCCGTC CCTGTCCCTG TCCCCCTTCC TGCCCCTGCT 
CCTGCCCCTC ATGGTGAGGC CCGTATCCTC ACAGTTACCC CCAGTAACCA ACTCCAGGGG 
CTGCCTCTGA CCCCTCCTGT GGTGGTGACC CATGGGGTGC AGATTGTGCA CTCCAGCGGG 
GAGCTGTTTC AAGAGTACCG GTACGGCGAC ATCCGCACCT ACCACCCCCC GGCCCAGCTC 
ACACACACTC AGTTTCCCGC CGCTTCCTCT GTTGGCCTGC CTTCCCGGAC CAAGACAGCT 
GCTCAGGGCC CTCCTCCTGA AGGTGAGCCC CTGCAGCCTC CTCAGCCTGT GCAGTCCACA 
CAGCCTGCCC AGCCTGCACC ACCCTGCCCG CCCTCCCAGC TCGGTCAGCC CGGCCAGCCA 
CCAAGCAGCA AGATGCCTCA AGTGTCCCAG GAGGCAAAGG GGACCCAGAC GGGAGTAGAG 
CAGCCTCGCC TCCCAGCTGG ACCTGCAAAC AGGCCACCTG AGCCTCACAC CCAGGTTCAG 
AGGGCACAAG CAGAAACAGG CCCGACTTCC TTCCCCTCCC CTGTGTCTGT CTCCATGAAG 
CCTGACCTTC CAGTCTCTCT TCCCACTCAG ACTGCCCCAA AACAGCCGTT GTTTGTCCCA 
ACAACCTCTG GCCCCAGCAC CCCACCAGGA CTGGTTCTGC CACACACTGA ATTCCAGCCA 
GCCCCCAAAC AAGATTCCTC TCCACACCTG ACTTCCCAGA GACCCGTGGA TATGGTTCAA 
CTTCTGAAGA AGTACCCCAT CGTGTGGCAG GGCCTGCTGG CCCTCAAGAA TGACACAGCT 
GCTGTGCAGC TCCACTTCGT CTCTGGCAAC AACGTCCTGG CCCATCGGTC CCTGCCCCTT 
TCTGAAGGAG GGCCCCCACT AAGGATCGCC CAGAGGATGC GGCTGGAGGC AACGCAGCTG 
GAAGGGGTTG CCCGAAGGAT GACGGTGGAG ACAGATTACT GTCTGCTGCT GGCTCTGCCC 
TGTGGCCGTG ACCAAGAGGA TGTTGTGAGC CAGACCGAGT CCCTCAAGGC TGCCTTCATC 
ACTTACCTGC AGGCCAAGCA GGCGGCAGGG ATCATCAACG TTCCCAACCC TGGCTCCAAT 
CAGCCTGCCT ACGTGCTGCA GATCTTCCCG CCCTGTGAGT TCTCTGAGAG TCACCTGTCC 
CGCCTGGCCC CTGACCTCCT TGCCAGCATC TCCAACATCT CTCCCCACCT CATGATTGTC 
ATTGCCTCCG TGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_055816.2
CDS	362..11356
Translation	MVRETRHLWVGNLPENVREEKIIEHFKRYGRVESVKILPKRGSEGGVAAFVDFVDIKSAQKAHNSVNKMGDRDLRTDYNEPGTIPSAARGLDDTVSIASRSREVSGFRGGGGGPAYGPPPSLHAREGRYERRLDGASDNRERAYEHSAYGHHERGTGGFDRTRHYDQDYYRDPRERTLQHGLYYASRSRSPNRFDAHDPRYEPRAREQFTLPSVVHRDIYRDDITREVRGRRPERNYQHSRSRSPHSSQSRNQSPQRLASQASRPTRSPSGSGSRSRSSSSDSISSSSSTSSDSSDSSSSSSDDSPARSVQSAAVPAPTSQLLSSLEKDEPRKSFGIKVQNLPVRSTDTSLKDGLFHEFKKFGKVTSVQIHGTSEERYGLVFFRQQEDQEKALTASKGKLFFGMQIEVTAWIGPETESENEFRPLDERIDEFHPKATRTLFIGNLEKTTTYHDLRNIFQRFGEIVDIDIKKVNGVPQYAFLQYCDIASVCKAIKKMDGEYLGNNRLKLGFGKSMPTNCVWLDGLSSNVSDQYLTRHFCRYGPVVKVVFDRLKGMALVLYNEIEYAQAAVKETKGRKIGGNKIKVDFANRESQLAFYHCMEKSGQDIRDFYEMLAERREERRASYDYNQDRTYYESVRTPGTYPEDSRRDYPARGREFYSEWETYQGDYYESRYYDDPREYRDYRNDPYEQDIREYSYRQRERERERERFESDRDRDHERRPIERSQSPVHLRRPQSPGASPSQAERLPSDSERRLYSRSSDRSGSCSSLSPPRYEKLDKSRLERYTKNEKTDKERTFDPERVERERRLIRKEKVEKDKTDKQKRKGKVHSPSSQSSETDQENEREQSPEKPRSCNKLSREKADKEGIAKNRLELMPCVVLTRVKEKEGKVIDHTPVEKLKAKLDNDTVKSSALDQKLQVSQTEPAKSDLSKLESVRMKVPKEKGLSSHVEVVEKEGRLKARKHLKPEQPADGVSAVDLEKLEARKRRFADSNLKAEKQKPEVKKSSPEMEDARVLSKKQPDVSSREVILLREGEAERKPVRKEILKRESKKIKLDRLNTVASPKDCQELASISVGSGSRPSSDLQARLGELAGESVENQEVQSKKPIPSKPQLKQLQVLDDQGPEREDVRKNYCSLRDETPERKSGQEKSHSVNTEEKIGIDIDHTQSYRKQMEQSRRKQQMEMEIAKSEKFGSPKKDVDEYERRSLVHEVGKPPQDVTDDSPPSKKKRMDHVDFDICTKRERNYRSSRQISEDSERTGGSPSVRHGSFHEDEDPIGSPRLLSVKGSPKVDEKVLPYSNITVREESLKFNPYDSSRREQMADMAKIKLSVLNSEDELNRWDSQMKQDAGRFDVSFPNSIIKRDSLRKRSVRDLEPGEVPSDSDEDGEHKSHSPRASALYESSRLSFLLRDREDKLRERDERLSSSLERNKFYSFALDKTITPDTKALLERAKSLSSSREENWSFLDWDSRFANFRNNKDKEKVDSAPRPIPSWYMKKKKIRTDSEGKMDDKKEDHKEEEQERQELFASRFLHSSIFEQDSKRLQHLERKEEDSDFISGRIYGKQTSEGANSTTDSIQEPVVLFHSRFMELTRMQQKEKEKDQKPKEVEKQEDTENHPKTPESAPENKDSELKTPPSVGPPSVTVVTLESAPSALEKTTGDKTVEAPLVTEEKTVEPATVSEEAKPASEPAPAPVEQLEQVDLPPGADPDKEAAMMPAGVEEGSSGDQPPYLDAKPPTPGASFSQAESNVDPEPDSTQPLSKPAQKSEEANEPKAEKPDATADAEPDANQKAEAAPESQPPASEDLEVDPPVAAKDKKPNKSKRSKTPVQAAAVSIVEKPVTRKSERIDREKLKRSNSPRGEAQKLLELKMEAEKITRTASKNSAADLEHPEPSLPLSRTRRRNVRSVYATMGDHENRSPVKEPVEQPRVTRKRLERELQEAAAVPTTPRRGRPPKTRRRADEEEENEAKEPAETLKPPEGWRSPRSQKTAAGGGPQGKKGKNEPKVDATRPEATTEVGPQIGVKESSMEPKAAEEEAGSEQKRDRKDAGTDKNPPETAPVEVVEKKPAPEKNSKSKRGRSRNSRLAVDKSASLKNVDAAVSPRGAAAQAGERESGVVAVSPEKSESPQKEDGLSSQLKSDPVDPDKEPEKEDVSASGPSPEATQLAKQMELEQAVEHIAKLAEASASAAYKADAPEGLAPEDRDKPAHQASETELAAAIGSIINDISGEPENFPAPPPYPGESQTDLQPPAGAQALQPSEEGMETDEAVSGILETEAATESSRPPVNAPDPSAGPTDTKEARGNSSETSHSVPEAKGSKEVEVTLVRKDKGRQKTTRSRRKRNTNKKVVAPVESHVPESNQAQGESPAANEGTTVQHPEAPQEEKQSEKPHSTPPQSCTSDLSKIPSTENSSQEISVEERTPTKASVPPDLPPPPQPAPVDEEPQARFRVHSIIESDPVTPPSDPSIPIPTLPSVTAAKLSPPVASGGIPHQSPPTKVTEWITRQEEPRAQSTPSPALPPDTKASDVDTSSSTLRKILMDPKYVSATSVTSTSVTTAIAEPVSAAPCLHEAPPPPVDSKKPLEEKTAPPVTNNSEIQASEVLVAADKEKVAPVIAPKITSVISRMPVSIDLENSQKITLAKPAPQTLTGLVSALTGLVNVSLVPVNALKGPVKGSVTTLKSLVSTPAGPVNVLKGPVNVLTGPVNVLTTPVNATVGTVNAAPGTVNAAASAVNATASAVTVTAGAVTAASGGVTATTGTVTMAGAVIAPSTKCKQRASANENSRFHPGSMPVIDDRPADAGSGAGLRVNTSEGVVLLSYSGQKTEGPQRISAKISQIPPASAMDIEFQQSVSKSQVKPDSVTASQPPSKGPQAPAGYANVATHSTLVLTAQTYNASPVISSVKADRPSLEKPEPIHLSVSTPVTQGGTVKVLTQGINTPPVLVHNQLVLTPSIVTTNKKLADPVTLKIETKVLQPANLGSTLTPHHPPALPSKLPTEVNHVPSGPSIPADRTVSHLAAAKLDAHSPRPSGPGPSSFPRASHPSSTASTALSTNATVMLAAGIPVPQFISSIHPEQSVIMPPHSITQTVSLSHLSQGEVRMNTPTLPSITYSIRPEALHSPRAPLQPQQIEVRAPQRASTPQPAPAGVPALASQHPPEEEVHYHLPVARATAPVQSEVLVMQSEYRLHPYTVPRDVRIMVHPHVTAVSEQPRAADGVVKVPPASKAPQQPGKEAAKTPDAKAAPTPTPAPVPVPVPLPAPAPAPHGEARILTVTPSNQLQGLPLTPPVVVTHGVQIVHSSGELFQEYRYGDIRTYHPPAQLTHTQFPAASSVGLPSRTKTAAQGPPPEGEPLQPPQPVQSTQPAQPAPPCPPSQLGQPGQPPSSKMPQVSQEAKGTQTGVEQPRLPAGPANRPPEPHTQVQRAQAETGPTSFPSPVSVSMKPDLPVSLPTQTAPKQPLFVPTTSGPSTPPGLVLPHTEFQPAPKQDSSPHLTSQRPVDMVQLLKKYPIVWQGLLALKNDTAAVQLHFVSGNNVLAHRSLPLSEGGPPLRIAQRMRLEATQLEGVARRMTVETDYCLLLALPCGRDQEDVVSQTESLKAAFITYLQAKQAAGIINVPNPGSNQPAYVLQIFPPCEFSESHLSRLAPDLLASISNISPHLMIVIASV

Target ORF information:

RefSeq Version	NM_015001.3
Organism	Homo sapiens(human)
Definition	Homo sapiens spen family transcriptional repressor (SPEN), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_015001.3

ORF Insert Sequence:

ATGGTCCGGG AAACCAGGCA TCTCTGGGTG GGCAACTTAC CCGAGAACGT GCGGGAAGAG 
AAGATCATCG AGCATTTCAA ACGATATGGC CGCGTGGAAA GTGTCAAAAT TCTTCCCAAG 
AGGGGATCTG AAGGAGGAGT GGCTGCCTTT GTGGATTTTG TGGACATCAA AAGTGCACAG 
AAAGCTCACA ACTCGGTCAA CAAAATGGGT GACAGAGACC TACGCACGGA TTATAATGAA 
CCAGGCACCA TCCCGAGTGC TGCTCGGGGA TTGGATGATA CAGTTTCCAT AGCATCTCGT 
AGTAGAGAGG TTTCTGGGTT CAGAGGAGGT GGTGGAGGGC CTGCTTATGG TCCCCCACCG 
TCACTTCATG CACGAGAAGG ACGTTATGAG CGGAGACTTG ATGGGGCTTC AGATAACAGG 
GAGCGTGCTT ATGAACATAG TGCCTATGGA CACCATGAAC GGGGGACGGG AGGATTTGAT 
CGGACAAGAC ATTACGATCA GGATTACTAT AGAGATCCTC GAGAGCGGAC TTTACAACAT 
GGGCTCTATT ACGCTTCTCG GAGTCGAAGT CCAAATCGCT TTGATGCTCA TGACCCCCGA 
TATGAACCTA GGGCTCGCGA GCAGTTTACA CTGCCCAGTG TGGTACACAG GGATATCTAC 
AGGGATGATA TTACCCGGGA GGTACGAGGC AGAAGGCCAG AGCGGAATTA CCAGCACAGC 
AGGAGTCGGT CACCACATTC ATCCCAGTCT AGAAATCAGT CTCCTCAGAG ACTGGCTAGC 
CAAGCATCTA GACCCACAAG GTCCCCTAGC GGCAGCGGCT CTAGAAGTAG ATCCTCCAGT 
AGTGATTCAA TCAGCAGCAG CAGTAGTACC AGCAGTGACA GCAGTGATTC CAGCAGTAGT 
TCAAGTGATG ATTCTCCAGC TCGATCAGTT CAGTCTGCAG CAGTCCCTGC ACCCACTTCC 
CAGTTGCTTT CATCTCTGGA AAAAGATGAG CCCCGTAAAA GTTTTGGCAT CAAGGTCCAG 
AATCTTCCAG TACGCTCTAC AGATACAAGC CTTAAAGATG GCCTTTTCCA TGAATTTAAG 
AAATTTGGAA AAGTAACTTC AGTGCAGATA CATGGAACTT CAGAAGAGAG GTATGGTCTG 
GTATTCTTTC GGCAGCAAGA GGACCAAGAA AAAGCCTTGA CTGCATCAAA AGGAAAACTT 
TTCTTTGGCA TGCAGATTGA AGTAACAGCA TGGATAGGTC CAGAAACAGA AAGTGAAAAT 
GAATTTCGCC CCTTGGATGA AAGGATAGAT GAATTTCACC CCAAAGCAAC AAGAACTCTC 
TTTATTGGCA ACCTTGAAAA AACCACTACT TACCATGACC TTCGCAACAT CTTCCAGCGC 
TTTGGAGAAA TTGTGGATAT TGACATTAAG AAAGTAAATG GAGTTCCTCA GTATGCGTTT 
CTGCAATACT GTGATATTGC TAGCGTTTGT AAAGCTATTA AGAAGATGGA TGGGGAATAT 
CTTGGAAATA ATCGCCTCAA GCTGGGTTTT GGAAAGAGCA TGCCTACAAA CTGCGTGTGG 
CTAGATGGGC TTTCTTCGAA TGTGTCAGAT CAGTATTTAA CACGACATTT CTGCCGATAT 
GGGCCTGTGG TAAAGGTGGT GTTTGACCGC TTAAAAGGCA TGGCCCTGGT TCTCTACAAT 
GAAATTGAAT ATGCACAAGC AGCTGTAAAA GAGACCAAAG GGAGGAAAAT CGGTGGGAAT 
AAAATTAAGG TGGATTTTGC AAATCGGGAA AGTCAGCTGG CTTTTTATCA CTGCATGGAG 
AAATCTGGTC AAGACATCAG AGACTTTTAT GAAATGTTAG CCGAAAGAAG AGAGGAACGA 
AGGGCATCCT ACGACTATAA CCAAGATCGT ACATATTATG AGAGTGTTCG AACTCCAGGC 
ACTTATCCTG AGGATTCCAG GCGGGACTAT CCAGCTCGAG GGAGAGAGTT TTATTCAGAA 
TGGGAAACTT ACCAAGGAGA CTACTATGAA TCACGATACT ACGATGATCC TCGGGAATAC 
AGGGATTACA GGAATGATCC TTATGAACAA GATATTAGGG AATATAGTTA CAGGCAAAGG 
GAACGAGAAA GAGAACGTGA AAGATTTGAG TCTGACCGGG ACAGAGACCA TGAGAGGAGG 
CCGATTGAAC GAAGTCAAAG TCCTGTTCAC TTGCGACGTC CACAGAGTCC TGGAGCGTCT 
CCCTCTCAGG CAGAGAGGTT GCCGAGTGAT TCTGAGAGGA GGCTTTACAG CCGATCCTCA 
GACCGGAGTG GAAGCTGTAG CTCACTCTCC CCTCCAAGAT ATGAGAAACT GGACAAGTCT 
CGTTTGGAGC GCTATACAAA AAATGAAAAG ACAGATAAAG AACGAACTTT TGATCCGGAG 
AGAGTGGAGA GAGAGAGACG CTTAATACGG AAGGAAAAAG TGGAAAAGGA CAAAACTGAC 
AAGCAGAAAC GCAAAGGAAA GGTTCACTCC CCTAGTTCTC AGTCTTCAGA AACGGACCAA 
GAAAATGAGC GAGAGCAAAG CCCTGAAAAG CCCAGGAGTT GTAATAAACT GAGCAGAGAG 
AAAGCTGACA AAGAGGGAAT AGCGAAAAAC CGCCTGGAAC TCATGCCTTG CGTGGTTTTG 
ACTCGAGTGA AAGAGAAAGA GGGAAAGGTC ATTGACCACA CTCCTGTGGA AAAGTTGAAA 
GCCAAGCTTG ATAATGACAC TGTCAAATCT TCTGCCCTGG ACCAGAAACT TCAGGTCTCT 
CAGACGGAGC CTGCAAAATC TGACTTGTCT AAACTGGAAT CAGTTAGAAT GAAAGTACCA 
AAGGAAAAGG GGCTTTCAAG CCATGTTGAA GTGGTGGAGA AGGAAGGCAG GCTTAAAGCC 
AGGAAGCACC TCAAGCCTGA GCAGCCTGCA GATGGGGTAA GTGCTGTGGA TCTGGAGAAG 
CTGGAAGCCA GGAAAAGGCG CTTTGCAGAT TCCAATTTAA AAGCAGAAAA GCAAAAACCA 
GAGGTCAAGA AAAGCAGTCC AGAGATGGAG GATGCTCGCG TGCTTTCAAA AAAGCAGCCT 
GACGTGTCCT CTAGAGAGGT CATTCTGCTG AGGGAAGGAG AGGCTGAAAG AAAGCCTGTG 
AGGAAAGAAA TTCTTAAAAG AGAATCTAAA AAAATCAAAC TGGACAGACT TAATACTGTT 
GCCAGCCCCA AAGACTGTCA GGAGCTTGCC AGTATTTCTG TTGGGTCTGG CTCAAGGCCC 
AGCTCAGACC TACAAGCAAG ACTGGGAGAA CTAGCAGGTG AATCTGTGGA AAATCAAGAA 
GTCCAATCAA AAAAGCCCAT TCCCTCAAAA CCACAGCTCA AACAGCTGCA GGTATTAGAT 
GATCAAGGAC CAGAGAGAGA AGACGTTAGG AAAAACTATT GCAGTCTTCG TGATGAAACA 
CCTGAACGTA AATCAGGCCA AGAGAAATCA CATTCAGTAA ATACTGAAGA AAAAATTGGC 
ATTGACATCG ATCACACGCA GAGTTACCGA AAACAAATGG AACAGAGTCG TAGGAAACAG 
CAGATGGAAA TGGAAATAGC CAAGTCTGAG AAGTTTGGCA GTCCTAAAAA AGATGTAGAT 
GAATATGAAA GACGTAGCCT CGTTCACGAG GTAGGCAAAC CCCCTCAAGA TGTCACTGAT 
GACTCTCCTC CTAGCAAAAA GAAAAGGATG GATCATGTCG ATTTTGATAT CTGCACCAAG 
CGAGAACGGA ATTACAGAAG TTCACGCCAA ATCAGCGAAG ATTCTGAAAG GACTGGTGGT 
TCTCCCAGTG TCCGACATGG TTCCTTCCAT GAAGATGAGG ATCCCATAGG CTCCCCTAGG 
CTACTGTCAG TAAAAGGGTC TCCTAAAGTA GATGAAAAAG TCCTCCCCTA TTCTAACATA 
ACAGTCAGGG AAGAGTCTTT AAAATTTAAT CCTTATGATT CTAGCAGGAG AGAACAGATG 
GCAGATATGG CCAAAATAAA ACTATCTGTC TTGAATTCTG AAGATGAACT AAATCGTTGG 
GACTCTCAGA TGAAACAGGA TGCTGGCAGA TTTGATGTGA GTTTCCCAAA CAGCATAATT 
AAGAGAGATA GCCTTCGAAA AAGGTCTGTA CGAGATCTGG AACCTGGTGA GGTGCCTTCT 
GATTCTGACG AAGATGGTGA ACACAAATCC CACTCACCCA GAGCCTCTGC ATTATATGAA 
AGTTCTCGAT TGTCTTTTTT ATTGAGGGAC AGAGAAGACA AGCTACGTGA GCGAGATGAA 
AGACTCTCTA GTTCTTTAGA AAGGAACAAA TTTTACTCTT TTGCATTGGA TAAGACAATC 
ACACCAGACA CTAAAGCTTT GCTTGAAAGA GCTAAATCCC TCTCTTCATC TCGTGAAGAA 
AATTGGTCTT TTCTTGATTG GGACTCCCGA TTTGCAAATT TTCGAAACAA CAAAGATAAA 
GAAAAGGTTG ACTCTGCTCC AAGACCTATT CCATCCTGGT ACATGAAAAA GAAGAAAATT 
AGGACTGATT CAGAAGGGAA AATGGATGAT AAGAAAGAGG ACCATAAAGA AGAAGAGCAA 
GAGAGGCAGG AATTGTTTGC TTCTCGTTTT TTACACAGCT CAATCTTTGA ACAAGATTCC 
AAGCGATTGC AGCATCTAGA GAGAAAAGAG GAAGATTCTG ACTTCATTTC TGGTAGGATC 
TATGGGAAGC AGACATCTGA GGGAGCAAAC AGCACAACTG ATTCCATTCA AGAACCAGTA 
GTTCTGTTCC ATAGCAGATT TATGGAGCTC ACACGGATGC AACAGAAAGA AAAAGAAAAA 
GACCAGAAAC CCAAAGAGGT TGAGAAACAG GAAGATACAG AGAATCATCC CAAGACCCCA 
GAATCTGCTC CTGAGAATAA AGATTCAGAA CTGAAAACTC CACCTTCCGT TGGGCCTCCA 
AGTGTCACAG TCGTAACTCT AGAATCAGCC CCATCAGCAC TAGAGAAGAC CACTGGTGAC 
AAAACGGTAG AGGCGCCTTT GGTAACAGAA GAGAAGACTG TGGAGCCAGC TACCGTCTCA 
GAAGAAGCAA AGCCTGCATC TGAACCTGCT CCTGCCCCTG TGGAACAGCT GGAACAAGTA 
GACCTGCCCC CAGGAGCAGA CCCCGATAAA GAAGCTGCCA TGATGCCTGC GGGTGTTGAG 
GAAGGTTCAT CAGGTGACCA GCCGCCTTAT CTGGATGCCA AGCCTCCAAC TCCCGGGGCC 
TCGTTTTCCC AGGCAGAGAG CAACGTAGAT CCAGAGCCTG ACAGTACCCA GCCACTTTCA 
AAACCAGCTC AGAAGTCTGA GGAAGCCAAT GAGCCAAAGG CCGAAAAGCC AGACGCCACT 
GCAGATGCTG AGCCTGATGC AAACCAGAAA GCCGAAGCTG CTCCTGAGTC TCAGCCCCCA 
GCTTCTGAAG ATTTAGAGGT TGATCCTCCA GTTGCTGCAA AGGATAAAAA GCCAAACAAA 
AGCAAGCGTT CAAAGACCCC TGTTCAGGCA GCTGCAGTGA GTATCGTGGA GAAGCCCGTC 
ACAAGGAAGA GTGAGAGGAT AGACCGGGAA AAACTCAAGC GGTCCAATTC TCCTCGGGGA 
GAAGCACAGA AGCTTTTGGA ATTGAAGATG GAGGCAGAGA AGATTACAAG GACTGCTTCT 
AAAAACTCTG CTGCAGACCT TGAACATCCC GAACCAAGTT TGCCTCTCAG CCGAACAAGG 
CGCCGGAATG TAAGGAGCGT CTATGCAACC ATGGGTGACC ATGAAAACCG CTCTCCTGTC 
AAAGAGCCCG TTGAGCAACC AAGAGTGACC AGAAAGAGAT TGGAGCGAGA GCTTCAGGAG 
GCTGCAGCGG TTCCCACCAC CCCTCGGAGG GGAAGGCCTC CAAAGACACG CCGGCGAGCC 
GATGAAGAGG AGGAGAACGA GGCCAAGGAA CCTGCAGAAA CACTCAAGCC ACCTGAGGGA 
TGGCGGTCGC CAAGGTCCCA GAAAACTGCA GCTGGTGGTG GACCCCAAGG GAAAAAGGGA 
AAAAATGAAC CGAAGGTGGA TGCTACACGT CCTGAGGCCA CCACTGAGGT GGGCCCCCAA 
ATAGGCGTGA AAGAGAGCTC CATGGAACCC AAGGCTGCTG AGGAGGAGGC AGGGAGTGAA 
CAGAAACGTG ACAGAAAAGA TGCTGGCACA GACAAAAACC CCCCTGAAAC CGCCCCTGTT 
GAAGTTGTAG AGAAAAAACC GGCCCCTGAA AAAAACTCCA AATCAAAGAG AGGAAGATCT 
CGAAACTCCA GGTTAGCAGT GGACAAATCT GCAAGTCTGA AAAATGTGGA TGCTGCTGTC 
AGTCCCAGGG GGGCTGCAGC ACAGGCAGGG GAGAGGGAAT CTGGGGTGGT GGCAGTCTCC 
CCTGAGAAAA GTGAGAGTCC CCAAAAGGAG GATGGTTTAT CATCCCAGTT GAAAAGTGAT 
CCAGTTGATC CAGACAAGGA ACCAGAGAAA GAAGACGTGT CTGCCTCTGG GCCGTCCCCA 
GAAGCCACCC AGTTAGCCAA GCAGATGGAG CTGGAGCAGG CCGTGGAACA CATCGCAAAG 
CTCGCTGAGG CCTCTGCCTC TGCTGCCTAT AAGGCAGATG CACCAGAGGG CCTTGCCCCA 
GAGGACAGGG ACAAGCCTGC ACACCAAGCA AGTGAAACAG AGCTGGCTGC GGCCATCGGC 
TCCATCATCA ATGACATTTC TGGGGAGCCA GAAAACTTCC CAGCACCTCC ACCTTATCCT 
GGAGAATCCC AGACAGATCT GCAACCCCCC GCAGGTGCAC AGGCGCTGCA GCCTTCTGAG 
GAAGGAATGG AGACAGATGA GGCTGTATCT GGCATCCTGG AAACTGAGGC TGCTACAGAA 
TCTTCTAGGC CTCCAGTCAA TGCTCCTGAC CCCTCAGCCG GCCCAACAGA TACCAAGGAA 
GCCAGAGGAA ATAGCAGTGA AACCTCACAC TCAGTGCCAG AAGCCAAAGG GTCTAAAGAA 
GTGGAAGTCA CTCTTGTTCG GAAAGACAAA GGGCGCCAGA AAACAACCCG ATCACGCCGC 
AAGCGAAACA CAAACAAGAA AGTGGTGGCT CCTGTAGAGA GCCATGTCCC TGAATCCAAC 
CAAGCTCAAG GTGAGAGTCC TGCTGCAAAT GAGGGGACAA CAGTACAGCA CCCCGAAGCC 
CCACAGGAAG AAAAGCAGAG TGAGAAACCC CATTCCACTC CTCCTCAGTC ATGTACTTCT 
GACCTAAGCA AGATTCCCTC CACAGAGAAT TCGTCCCAAG AAATCAGTGT TGAGGAAAGG 
ACTCCAACCA AAGCATCTGT GCCCCCAGAC CTTCCCCCAC CTCCCCAGCC AGCACCGGTG 
GATGAGGAGC CTCAAGCCAG GTTCAGGGTG CATTCCATCA TTGAAAGTGA CCCGGTGACC 
CCACCCAGCG ATCCAAGCAT CCCCATACCC ACACTGCCTT CTGTAACTGC AGCAAAGCTC 
TCACCTCCTG TCGCCTCTGG GGGGATCCCA CACCAGAGCC CCCCTACTAA GGTGACAGAG 
TGGATCACAA GGCAGGAGGA GCCACGGGCT CAGTCTACTC CATCTCCAGC TCTTCCCCCA 
GACACAAAGG CCTCTGATGT TGACACCAGC TCCAGCACCC TGAGGAAGAT TCTCATGGAC 
CCCAAGTATG TGTCTGCCAC AAGTGTCACT TCCACAAGTG TCACCACAGC CATTGCAGAG 
CCTGTCAGTG CTGCCCCTTG CCTACATGAG GCCCCGCCCC CGCCAGTTGA CTCTAAAAAG 
CCTTTAGAAG AAAAAACAGC ACCTCCAGTG ACAAACAACT CTGAGATACA AGCCTCGGAG 
GTGCTGGTAG CTGCTGACAA GGAAAAGGTG GCTCCAGTCA TTGCTCCCAA AATTACCTCT 
GTTATTAGCC GGATGCCTGT CAGCATTGAC CTGGAAAATT CACAGAAGAT AACCTTGGCA 
AAACCAGCTC CTCAAACCCT CACTGGTCTG GTGAGCGCAC TCACTGGCCT GGTGAACGTC 
TCCCTGGTCC CGGTGAATGC CCTGAAAGGC CCCGTGAAGG GCTCAGTGAC CACACTGAAA 
AGTTTGGTGA GCACCCCTGC TGGGCCCGTG AACGTCCTGA AAGGGCCTGT GAATGTTCTT 
ACGGGGCCAG TGAATGTTCT CACCACTCCA GTGAACGCCA CGGTGGGCAC AGTGAATGCC 
GCCCCAGGCA CAGTCAATGC CGCTGCGAGT GCAGTGAATG CCACAGCAAG TGCAGTGACC 
GTCACAGCGG GTGCGGTTAC TGCTGCATCT GGTGGTGTAA CGGCCACAAC AGGCACGGTG 
ACAATGGCAG GGGCAGTGAT TGCGCCGTCA ACAAAGTGCA AACAGAGAGC GAGTGCTAAT 
GAAAACAGTC GGTTCCACCC AGGGTCCATG CCTGTGATCG ACGATCGTCC GGCAGACGCG 
GGCTCAGGGG CGGGGCTGCG TGTGAACACT TCTGAAGGGG TTGTGCTCCT GAGTTACTCA 
GGGCAGAAGA CCGAAGGCCC ACAGCGGATC AGCGCCAAGA TCAGCCAGAT CCCCCCGGCC 
AGTGCAATGG ACATTGAATT TCAGCAGTCA GTGTCCAAGT CCCAGGTCAA ACCTGATTCT 
GTCACAGCAT CGCAGCCTCC ATCCAAAGGC CCTCAAGCTC CTGCAGGCTA TGCGAACGTG 
GCCACCCATT CCACGTTGGT ACTGACCGCC CAGACATATA ATGCCTCTCC TGTGATTTCG 
TCTGTGAAGG CCGATAGGCC ATCCTTGGAG AAGCCCGAGC CCATTCACCT CTCGGTGTCC 
ACGCCTGTCA CCCAGGGAGG CACAGTGAAG GTTCTCACCC AGGGGATCAA CACACCCCCT 
GTGCTGGTTC ACAACCAGCT GGTCCTCACC CCAAGCATTG TCACCACAAA CAAGAAGCTT 
GCTGACCCCG TCACCCTTAA AATCGAGACC AAGGTCCTTC AGCCGGCCAA CCTGGGGTCC 
ACGCTCACGC CCCACCACCC TCCTGCTCTG CCCAGCAAAC TGCCTACAGA AGTCAACCAT 
GTCCCCTCGG GGCCCAGCAT CCCAGCAGAT CGAACTGTCT CCCATTTGGC AGCTGCAAAG 
CTAGATGCTC ATTCTCCTCG ACCAAGTGGA CCCGGGCCAT CCTCATTCCC AAGGGCAAGC 
CACCCCAGCA GTACTGCATC TACGGCGCTC TCCACCAACG CCACAGTCAT GCTGGCTGCA 
GGCATCCCAG TGCCCCAGTT CATCTCCAGC ATCCACCCAG AGCAGTCTGT CATCATGCCA 
CCCCACAGCA TCACCCAGAC TGTGTCCCTG AGCCACCTCT CCCAGGGCGA GGTGAGAATG 
AACACTCCCA CGCTGCCCAG TATCACCTAC AGCATCCGGC CAGAAGCGCT TCACTCTCCT 
CGGGCTCCGC TGCAGCCCCA GCAAATAGAG GTCAGGGCCC CACAGCGTGC CAGCACCCCG 
CAGCCAGCCC CAGCTGGTGT GCCTGCACTG GCCTCCCAGC ACCCTCCCGA GGAGGAAGTG 
CATTATCACC TTCCTGTCGC TCGAGCCACA GCCCCTGTGC AGTCAGAGGT ACTAGTCATG 
CAGTCTGAGT ACCGACTGCA CCCCTATACT GTGCCACGGG ATGTGAGGAT CATGGTGCAT 
CCACATGTGA CGGCAGTCAG CGAGCAGCCC AGGGCCGCGG ATGGGGTGGT GAAGGTGCCA 
CCAGCCAGCA AGGCCCCTCA GCAGCCAGGG AAGGAAGCTG CCAAGACACC AGATGCCAAA 
GCTGCCCCCA CCCCCACCCC TGCCCCCGTC CCTGTCCCTG TCCCCCTTCC TGCCCCTGCT 
CCTGCCCCTC ATGGTGAGGC CCGTATCCTC ACAGTTACCC CCAGTAACCA ACTCCAGGGG 
CTGCCTCTGA CCCCTCCTGT GGTGGTGACC CATGGGGTGC AGATTGTGCA CTCCAGCGGG 
GAGCTGTTTC AAGAGTACCG GTACGGCGAC ATCCGCACCT ACCACCCCCC GGCCCAGCTC 
ACACACACTC AGTTTCCCGC CGCTTCCTCT GTTGGCCTGC CTTCCCGGAC CAAGACAGCT 
GCTCAGGGCC CTCCTCCTGA AGGTGAGCCC CTGCAGCCTC CTCAGCCTGT GCAGTCCACA 
CAGCCTGCCC AGCCTGCACC ACCCTGCCCG CCCTCCCAGC TCGGTCAGCC CGGCCAGCCA 
CCAAGCAGCA AGATGCCTCA AGTGTCCCAG GAGGCAAAGG GGACCCAGAC GGGAGTAGAG 
CAGCCTCGCC TCCCAGCTGG ACCTGCAAAC AGGCCACCTG AGCCTCACAC CCAGGTTCAG 
AGGGCACAAG CAGAAACAGG CCCGACTTCC TTCCCCTCCC CTGTGTCTGT CTCCATGAAG 
CCTGACCTTC CAGTCTCTCT TCCCACTCAG ACTGCCCCAA AACAGCCGTT GTTTGTCCCA 
ACAACCTCTG GCCCCAGCAC CCCACCAGGA CTGGTTCTGC CACACACTGA ATTCCAGCCA 
GCCCCCAAAC AAGATTCCTC TCCACACCTG ACTTCCCAGA GACCCGTGGA TATGGTTCAA 
CTTCTGAAGA AGTACCCCAT CGTGTGGCAG GGCCTGCTGG CCCTCAAGAA TGACACAGCT 
GCTGTGCAGC TCCACTTCGT CTCTGGCAAC AACGTCCTGG CCCATCGGTC CCTGCCCCTT 
TCTGAAGGAG GGCCCCCACT AAGGATCGCC CAGAGGATGC GGCTGGAGGC AACGCAGCTG 
GAAGGGGTTG CCCGAAGGAT GACGGTGGAG ACAGATTACT GTCTGCTGCT GGCTCTGCCC 
TGTGGCCGTG ACCAAGAGGA TGTTGTGAGC CAGACCGAGT CCCTCAAGGC TGCCTTCATC 
ACTTACCTGC AGGCCAAGCA GGCGGCAGGG ATCATCAACG TTCCCAACCC TGGCTCCAAT 
CAGCCTGCCT ACGTGCTGCA GATCTTCCCG CCCTGTGAGT TCTCTGAGAG TCACCTGTCC 
CGCCTGGCCC CTGACCTCCT TGCCAGCATC TCCAACATCT CTCCCCACCT CATGATTGTC 
ATTGCCTCCG TGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

SPEN, a new player in primary cilia formation and cell migration in breast cancer.
Breast cancer research : BCR19(1)104(2017 Sep)
L?gar? S,Chabot C,Basik M

The Estrogen Receptor Cofactor SPEN Functions as a Tumor Suppressor and Candidate Biomarker of Drug Responsiveness in Hormone-Dependent Breast Cancers.
Cancer research75(20)4351-63(2015 Oct)
L?gar? S,Cavallone L,Mamo A,Chabot C,Sirois I,Magliocco A,Klimowicz A,Tonin PN,Buchanan M,Keilty D,Hassan S,Laperri?re D,Mader S,Aleynikova O,Basik M

The NOTCH pathway is recurrently mutated in diffuse large B-cell lymphoma associated with hepatitis C virus infection.
Haematologica100(2)246-52(2015 Feb)
Arcaini L,Rossi D,Lucioni M,Nicola M,Bruscaggin A,Fiaccadori V,Riboni R,Ramponi A,Ferretti VV,Cresta S,Casaluci GM,Bonfichi M,Gotti M,Merli M,Maffi A,Arra M,Varettoni M,Rattotti S,Morello L,Guerrera ML,Sciarra R,Gaidano G,Cazzola M,Paulli M

Proteomic analyses reveal distinct chromatin-associated and soluble transcription factor complexes.
Molecular systems biology11(1)775(2015 Jan)
Li X,Wang W,Wang J,Malovannaya A,Xi Y,Li W,Guerra R,Hawke DH,Qin J,Chen J

The crystal structure of the Split End protein SHARP adds a new layer of complexity to proteins containing RNA recognition motifs.
Nucleic acids research42(10)6742-52(2014 Jun)
Arieti F,Gabus C,Tambalo M,Huet T,Round A,Thore S

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SPEN cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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