Slirp cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Slirp
Entrez Gene ID	688717
Full Name	SRA stem-loop interacting RNA binding protein
General protein information	Preferred Names SRA stem-loop interacting RNA binding protein Names SRA stem-loop-interacting RNA-binding protein, mitochondrial
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 6 Map Location: 6q31

mRNA and Protein(s)

mRNA	Protein	Name
NM_001109507.1	NP_001102977.1	SRA stem-loop-interacting RNA-binding protein, mitochondrial

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	SLIRP	XP_003952595.1
Homo sapiens (human)	SLIRP	NP_112487.1
Canis lupus familiaris (dog)	SLIRP	XP_547932.2
Rattus norvegicus (Norway rat)	Slirp	NP_001102977.1
Danio rerio (zebrafish)	si:ch73-276n11.1	XP_002665211.1
Xenopus tropicalis (tropical clawed frog)	slirp	XP_002938905.2
Bos taurus (cattle)	SLIRP	NP_001032562.1
Mus musculus (house mouse)	Slirp	NP_081234.2
Gallus gallus (chicken)	SLIRP	XP_001235249.1

Gene Ontology
Bibliography

Related articles in PubMed

Loss of the nuclear receptor corepressor SLIRP compromises male fertility.
Colley SM, Wintle L, Searles R, Russell V, Firman RC, Smith S, Deboer K, Merriner DJ, Genevieve B, Bentel JM, Stuckey BG, Phillips MR, Simmons LW, de Kretser DM, O'Bryan MK, Leedman PJ
PloS one8(8)e70700(2013)

Insights into RNA biology from an atlas of mammalian mRNA-binding proteins.
Castello A, Fischer B, Eichelbaum K, Horos R, Beckmann BM, Strein C, Davey NE, Humphreys DT, Preiss T, Steinmetz LM, Krijgsveld J, Hentze MW
Cell149(6)1393-406(2012 Jun)

The mRNA-bound proteome and its global occupancy profile on protein-coding transcripts.
Baltz AG, Munschauer M, Schwanh?usser B, Vasile A, Murakawa Y, Schueler M, Youngs N, Penfold-Brown D, Drew K, Milek M, Wyler E, Bonneau R, Selbach M, Dieterich C, Landthaler M
Molecular cell46(5)674-90(2012 Jun)

LRPPRC is necessary for polyadenylation and coordination of translation of mitochondrial mRNAs.
Ruzzenente B, Metodiev MD, Wredenberg A, Bratic A, Park CB, C?mara Y, Milenkovic D, Zickermann V, Wibom R, Hultenby K, Erdjument-Bromage H, Tempst P, Brandt U, Stewart JB, Gustafsson CM, Larsson NG
The EMBO journal31(2)443-56(2012 Jan)

Phosphoproteome analysis of functional mitochondria isolated from resting human muscle reveals extensive phosphorylation of inner membrane protein complexes and enzymes.
Zhao X, Le?n IR, Bak S, Mogensen M, Wrzesinski K, H?jlund K, Jensen ON
Molecular & cellular proteomics : MCP10(1)M110.000299(2011 Jan)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Slirp gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Slirp cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa10134	NM_001109507.1 Latest version!	Rattus norvegicus SRA stem-loop interacting RNA binding protein (Slirp), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$69.30 $99.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Slirp ( NM_001109507.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_001102977.1 Rattus norvegicus SRA stem-loop interacting RNA binding protein (Slirp), mRNA.

Price & Availability↑

CloneID	ORa10134
Clone ID Related Accession (Same CDS sequence)	NM_001109507.1
Accession Version	NM_001109507.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 336bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-27
Organism	Rattus norvegicus(Norway rat)
Product	SRA stem-loop-interacting RNA-binding protein, mitochondrial
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from CH473982.1. On Oct 4, 2007 this sequence version replaced XM_001068036.1. ##Evidence-Data-START## Transcript exon combination :: FQ237039.1, FQ211825.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMD00052296, SAMD00052297 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: inferred from homology ##RefSeq-Attributes-END##

ATGGCAGCCT CCGCGGTAAA AGGACTAACT GCGTTGCGTT CCCGTACTGG TCGGCCTATA 
GCGTTTATTA GAAAAATTCC TTGGACCGCG GCATCGAGTG AGCTAAGAGA ACATTTTGCT 
CAGTTTGGCC ATGTAAGAAG GTGCACTGTA CCTTTTGACA AAGAGACTGG CTTTCACAGA 
GGCATGGGTT GGGTTCAGTT TTCTTCACAG GAAGAACTTC AGAATGCACT ACAAGAAGAA 
CATCATATTA TTGATGGAGT AGAAATCCAT GTTCAACCTC AAAAACCAAA GGTTTTGCAC 
GGGGCTCAAA CATCTGATGA AGAGAAAGAT TTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001102977.1
CDS	99..434
Translation	MAASAVKGLTALRSRTGRPIAFIRKIPWTAASSELREHFAQFGHVRRCTVPFDKETGFHRGMGWVQFSSQEELQNALQEEHHIIDGVEIHVQPQKPKVLHGAQTSDEEKDF

Target ORF information:

RefSeq Version	NM_001109507.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus SRA stem-loop interacting RNA binding protein (Slirp), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001109507.1

ORF Insert Sequence:

ATGGCAGCCT CCGCGGTAAA AGGACTAACT GCGTTGCGTT CCCGTACTGG TCGGCCTATA 
GCGTTTATTA GAAAAATTCC TTGGACCGCG GCATCGAGTG AGCTAAGAGA ACATTTTGCT 
CAGTTTGGCC ATGTAAGAAG GTGCACTGTA CCTTTTGACA AAGAGACTGG CTTTCACAGA 
GGCATGGGTT GGGTTCAGTT TTCTTCACAG GAAGAACTTC AGAATGCACT ACAAGAAGAA 
CATCATATTA TTGATGGAGT AGAAATCCAT GTTCAACCTC AAAAACCAAA GGTTTTGCAC 
GGGGCTCAAA CATCTGATGA AGAGAAAGAT TTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Loss of the nuclear receptor corepressor SLIRP compromises male fertility.
PloS one8(8)e70700(2013)
Colley SM,Wintle L,Searles R,Russell V,Firman RC,Smith S,Deboer K,Merriner DJ,Genevieve B,Bentel JM,Stuckey BG,Phillips MR,Simmons LW,de Kretser DM,O'Bryan MK,Leedman PJ

Insights into RNA biology from an atlas of mammalian mRNA-binding proteins.
Cell149(6)1393-406(2012 Jun)
Castello A,Fischer B,Eichelbaum K,Horos R,Beckmann BM,Strein C,Davey NE,Humphreys DT,Preiss T,Steinmetz LM,Krijgsveld J,Hentze MW

The mRNA-bound proteome and its global occupancy profile on protein-coding transcripts.
Molecular cell46(5)674-90(2012 Jun)
Baltz AG,Munschauer M,Schwanh?usser B,Vasile A,Murakawa Y,Schueler M,Youngs N,Penfold-Brown D,Drew K,Milek M,Wyler E,Bonneau R,Selbach M,Dieterich C,Landthaler M

LRPPRC is necessary for polyadenylation and coordination of translation of mitochondrial mRNAs.
The EMBO journal31(2)443-56(2012 Jan)
Ruzzenente B,Metodiev MD,Wredenberg A,Bratic A,Park CB,C?mara Y,Milenkovic D,Zickermann V,Wibom R,Hultenby K,Erdjument-Bromage H,Tempst P,Brandt U,Stewart JB,Gustafsson CM,Larsson NG

Phosphoproteome analysis of functional mitochondria isolated from resting human muscle reveals extensive phosphorylation of inner membrane protein complexes and enzymes.
Molecular & cellular proteomics : MCP10(1)M110.000299(2011 Jan)
Zhao X,Le?n IR,Bak S,Mogensen M,Wrzesinski K,H?jlund K,Jensen ON

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Slirp cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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